Circulating heat exchange system for bacterial thermal cracking

Abstract

The utility model provides a circulating heat exchange system for bacterial thermal cracking. The circulating heat exchange system comprises a buffer solution stirring tank, a heat transfer copper coil pipe, a cooling copper coil pipe and a bacterial cracking solution collecting tank which are sequentially communicated through fluid, the heat transfer copper coil pipe is contained in the hot water tank and immersed in hot water, and the hot water tank is connected with a circulating hot water constant temperature system. The cooling copper coil pipe is contained in the cold water tank and immersed in cold water, and the cold water tank is connected with a circulating cold water constant temperature system. Through circulating heat exchange of the hot water tank and the cold water tank, the temperature variation of the hot water tank and the cold water tank can be conveniently reduced, and the cracking effect of resuspended bacterial liquid can be conveniently improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biopharmaceuticals, in particular to a circulating heat exchange system for bacterial thermal cracking. Background Art

[0002] Plasmid DNA is the most common delivery vehicle in genetic engineering, cell engineering, and protein engineering, and has been gradually applied in clinical trials and industrialization. Delivery vectors based on plasmid DNA are less effective in cell transfection, as only one out of every 1,000 plasmid molecules presented to cells can reach the cell nucleus and be expressed, while clinical applications require a large amount of plasmid DNA.

[0003] Currently, plasmid DNA is isolated from recombinant E. coli strains. The manufacturing process of plasmid DNA includes the selection and construction of a suitable expression vector, the growth of bacterial cells, the lysis of cells, the extraction of plasmids and the purification of plasmids.

[0004] Extraction of plasmid DNA from bacterial cells usually starts with chemical lysis. Common lysis methods for plasmid DNA are alkaline lysis and boiling lysis. Alkaline lysis technology is relatively mature and can process samples from 1ml to 500ml. It has a wide range of applications and can be used for small-scale laboratory research and industrial-scale large-scale production of clinical-grade plasmid DNA to a certain extent. It is applicable to all strains of Escherichia coli. However, the operation steps of the alkaline lysis method are difficult to control, and the alkaline lysis method has strict requirements on pH and is difficult to control; boiling lysis destroys the plasmid DNA at 100℃ and releases the plasmid DNA from the bacteria. Compared with traditional alkaline lysis technology, it can produce more plasmids, but the yield and purity of the plasmids are inconsistent.

[0005] Based on the boiling lysis scheme, the article "A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis" (Zhu, Kaichun, et al. "A continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis." Nature protocols 1.6 (2006): 3088-3093.) introduces a pipeline process for extracting plasmids by continuous thermal lysis, which includes the following steps: the resuspended bacterial solution in the fermentation tank is pumped into the heat exchange coil by a peristaltic pump, the cells are first continuously lysed in a 70°C water bath, then passed through an ice bath and collected, and the collected lysate is subjected to electrophoresis detection, and the detection results are as follows: Figure 5As shown, M1, DNA molecular weight marker B500352; 1, supernatant product after traditional thermal lysis of bacteria; 2, low-concentration supernatant product standard obtained after purification; 3, high-concentration supernatant product standard obtained after purification; M2, DNA molecular weight marker B500351. The above operation is convenient for observing the performance of the supernatant, and is convenient for simplifying the plasmid extraction process of Escherichia coli batch fermentation culture, and through the dual cooperation of the peristaltic pump and the heat transfer coil, it provides a streamlined operation process for bacterial cell lysis and plasmid extraction.

[0006] When the volume of the resuspended bacterial solution to be processed is large, as the resuspended bacterial solution continuously exchanges heat with the water in the water bath through the heat transfer coil, the cooling coil, the water temperature in the water bath deviates greatly from the initial value set initially. Therefore, as the lysis process continues, the lysis effect of the resuspended bacterial solution will decrease. Utility Model Content

[0007] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a circulating heat exchange system for bacterial thermal lysis.

[0008] To achieve the above-mentioned purpose and other related purposes, the utility model provides a circulating heat exchange system for bacterial thermal lysis, wherein the circulating heat exchange system comprises a buffer stirring tank, a heat transfer copper coil, a cooling copper coil and a bacterial lysis liquid collection tank which are fluidically connected in sequence; the heat transfer copper coil is accommodated in a hot water tank and immersed in hot water, and the hot water tank is connected to a circulating hot water constant temperature system; the cooling copper coil is accommodated in a cold water tank and immersed in cold water, and the cold water tank is connected to a circulating cold water constant temperature system.

[0009] In some preferred embodiments, the circulating hot water constant temperature system includes a constant temperature heat storage tank, a circulating hot water pipeline and a semiconductor heater, the hot water tank is provided with a circulating hot water inlet and a circulating hot water outlet, the circulating hot water outlet is connected to the inlet of the semiconductor heater via the circulating hot water pipeline, the outlet of the semiconductor heater is connected to the inlet of the constant temperature heat storage tank via the circulating hot water pipeline, and the outlet of the constant temperature heat storage tank is connected to the circulating hot water inlet of the hot water tank via the circulating hot water pipeline.

[0010] In some specific embodiments, a second peristaltic pump is provided on the circulating hot water pipeline between the outlet of the constant temperature hot water storage tank and the circulating hot water inlet of the hot water tank;

[0011] And / or, the constant temperature hot water storage tank is a multi-layer structure, which comprises an inner tank, a heat preservation plate and an outer shell from inside to outside.

[0012] In some specific embodiments, the semiconductor heater includes a semiconductor heat exchange component, a temperature sensor and a controller, the temperature sensor is communicatively connected to the controller, and the semiconductor heat exchange component is electrically connected to the controller.

[0013] In some preferred embodiments, the circulating cold water constant temperature system includes a constant temperature cold water storage tank, a circulating cold water pipeline and a semiconductor condenser. The cold water tank is provided with a circulating cold water inlet and a circulating cold water outlet. The circulating cold water outlet is connected to the inlet of the semiconductor condenser via a circulating cold water pipeline, the outlet of the semiconductor condenser is connected to the inlet of the constant temperature cold water storage tank via a circulating cold water pipeline, and the outlet of the constant temperature cold water storage tank is connected to the circulating cold water inlet of the cold water tank via a circulating cold water pipeline.

[0014] In some specific embodiments, a peristaltic pump 3 is provided on the circulating cold water pipeline between the outlet of the constant temperature cold water storage tank and the circulating cold water inlet of the cold water tank;

[0015] And / or, the constant temperature cold water storage tank is a multi-layer structure, which comprises an inner tank, a heat preservation plate and an outer shell from inside to outside.

[0016] In some specific embodiments, the buffer stirring tank is equipped with an electric stirring paddle;

[0017] And / or, the heat transfer copper coil is a hollow copper tube, and the heat transfer copper coil is arranged in multiple circles, and the diameter of each circle is 7-10 cm;

[0018] And / or, the cooling copper coil is a hollow copper tube, and the cooling copper coil is arranged in multiple circles, and the diameter of each circle is 7-10 cm.

[0019] In some preferred embodiments, the inner diameter of the heat transfer copper coil is 2-3 mm, and the outer diameter of the heat transfer copper coil is 4-5 mm;

[0020] And / or, the inner diameter of the cooling copper coil is 2-3 mm, and the outer diameter of the cooling copper coil is 4-5 mm.

[0021] In some preferred embodiments, a peristaltic pump is provided on the pipeline connecting the liquid outlet of the buffer stirring tank and the liquid inlet of the heat transfer copper coil.

[0022] As described above, the utility model of a circulating heat exchange system for bacterial thermal cracking has the following beneficial effects:

[0023] (1) The utility model realizes circulating heat exchange of the heat transfer copper coil and the cooling copper coil, which facilitates accurate temperature control of the heat transfer copper coil and the cooling copper coil, thereby making the bacterial liquid lysis effect after re-suspending better;

[0024] (2) The products obtained by the utility model are more stable and uniform between batches, and the quality is relatively better. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Shown is a schematic diagram of the structure of a circulating heat exchange system for bacterial thermal lysis according to the utility model.

[0026] Figure 2 Shown is a schematic diagram of the internal structure principle of the semiconductor heater of the present utility model.

[0027] Figure 3 Shown is a schematic diagram of the internal structure principle of the semiconductor condenser of the present utility model.

[0028] Figure 4 Shown is an agarose gel electrophoresis diagram of the supernatant product after lysis of the system of the utility model.

[0029] Figure 5 Shown is the agarose gel electrophoresis of the supernatant product after traditional thermal lysis of bacteria.

[0030] Figure 1-Figure 3 The accompanying drawings are denoted as follows:

[0031] 1 Buffer mixing tank

[0032] 2 Heat transfer copper coil

[0033] 3 Cooling copper coils

[0034] 4 Bacterial lysate collection tank

[0035] 5. Hot water tank

[0036] 6 Circulating hot water constant temperature system

[0037] 61 semiconductor heater

[0038] 62 Circulating hot water pipeline

[0039] 63 Constant temperature hot water storage tank

[0040] 64 Peristaltic Pump II

[0041] 7 Cold water tank

[0042] 8 Circulating cold water constant temperature system

[0043] 81 Constant temperature cold water storage tank

[0044] 82 Circulating cold water pipeline

[0045] 83 Semiconductor condenser

[0046] 84 Peristaltic Pump Three

[0047] 9 Peristaltic pump DETAILED DESCRIPTION

[0048] The following is an explanation of the implementation of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementations, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0049] See also Figures 1 to 3 It should be noted that the illustrations provided in this embodiment are only used to schematically illustrate the basic concept of the present invention. Therefore, the illustrations only show components related to the present invention rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0050] See also Figure 1-3 The utility model provides a circulating heat exchange system for bacterial thermal cracking.

[0051] See also Figure 1 A circulating heat exchange system for bacterial thermal lysis comprises a buffer stirring tank 1, a heat transfer copper coil 2, a cooling copper coil 3 and a bacterial lysis solution collection tank 4 which are sequentially fluid-connected; the heat transfer copper coil 2 is accommodated in a hot water tank 5 and immersed in hot water, and the hot water tank 5 is connected to a circulating hot water constant temperature system 6, which is convenient for realizing accurate control of the hot water temperature of the hot water tank 5; the cooling copper coil 3 is accommodated in a cold water tank 7 and immersed in cold water, and the cold water tank 7 is connected to a circulating cold water constant temperature system 8, which is convenient for realizing accurate control of the cold water temperature of the cold water tank 7 Precise control: Through the circulating heat exchange system, the resuspended bacterial liquid is stirred and mixed in the buffer stirring tank 1, and transported to the heat transfer copper coil 2 for thermal cracking. The heat transfer copper coil 2 is placed in the hot water tank 5. The circulating hot water constant temperature system 6 accurately controls the water temperature in the hot water tank 5. Then it is transported to the cooling copper coil 3 for cooling. The cooling copper coil 3 is placed in the cold water tank 7. The circulating cold water constant temperature system 8 accurately controls the water temperature in the cold water tank 7. After cooling, it is transported to the bacterial lysis liquid collection tank 4 for collection, which is convenient for realizing assembly line operation.

[0052] In some embodiments of the present invention, Figure 1 As shown, a peristaltic pump 9 is provided on the pipeline connecting the liquid outlet of the buffer stirring tank 1 and the liquid inlet of the heat transfer copper coil 2. The resuspended bacterial solution in the buffer stirring tank 1 is pumped to the heat transfer copper coil 2 by the peristaltic pump 9.

[0053] In some embodiments of the present invention, Figure 1As shown, the circulating hot water constant temperature system 6 includes a constant temperature heat storage tank 63, a circulating hot water pipeline 62 and a semiconductor heater 61. The hot water tank 5 is provided with a circulating hot water inlet and a circulating hot water outlet. The circulating hot water outlet is connected to the inlet of the semiconductor heater 61 through the circulating hot water pipeline 62. The outlet of the semiconductor heater 61 is connected to the inlet of the constant temperature heat storage tank 63 through the circulating hot water pipeline 62. The outlet of the constant temperature heat storage tank 63 is connected to the circulating hot water inlet of the hot water tank 5 through the circulating hot water pipeline 62. When the temperature of the hot water in the hot water tank 5 decreases, the hot water enters the semiconductor heater 61 from the circulating hot water outlet through the circulating hot water pipeline 62 for heating. The heated hot water is transmitted to the constant temperature heat storage tank 63 through the outlet of the semiconductor heater 61. The hot water in the constant temperature heat storage tank 63 enters the hot water tank 5 to realize the circulating heating process. The circulating hot water pipeline 62 is a rubber tube.

[0054] In some embodiments of the present invention, the constant temperature hot water storage tank 63 is a multi-layer structure, which includes an inner tank, an insulation board, and an outer shell from inside to outside; the inner tank is made of stainless steel; the outer shell is made of stainless steel, and the insulation board is a polystyrene board.

[0055] In some embodiments of the present invention, Figure 1 As shown, a peristaltic pump 64 is provided on the circulating hot water pipeline 62 between the outlet of the constant temperature hot water storage tank 63 and the circulating hot water inlet of the hot water tank 5 to facilitate control of the flow rate.

[0056] In some embodiments of the present invention, Figure 1 and Figure 2 As shown, the semiconductor heater 61 includes a semiconductor heat exchange component, a temperature sensor and a controller. The temperature sensor is connected to the controller in communication, and the semiconductor heat exchange component is electrically connected to the controller. The temperature sensor is used to monitor the temperature of the water in the hot water tank 5 in real time and feed it back to the controller in time. The controller inputs a heating instruction to the semiconductor heat exchange component. The semiconductor heat exchange component generates heat through the forward voltage power supply of the controller, thereby quickly performing the heating function. The controller is powered by low-voltage DC power.

[0057] In some embodiments of the present invention, Figure 1As shown, the circulating cold water constant temperature system 8 includes a constant temperature cold water storage tank 81, a circulating cold water pipeline 82 and a semiconductor condenser 83. The cold water tank 7 is provided with a circulating cold water inlet and a circulating cold water outlet. The circulating cold water outlet is connected to the inlet of the semiconductor condenser 83 through the circulating cold water pipeline 82. The outlet of the semiconductor condenser 83 is connected to the inlet of the constant temperature cold water storage tank 81 through the circulating cold water pipeline 82. The outlet of the constant temperature cold water storage tank 81 is connected to the circulating cold water inlet of the cold water tank 7 through the circulating cold water pipeline 82. When the temperature of the cold water in the cold water tank 7 increases, the cold water enters the semiconductor condenser 83 from the circulating cold water outlet through the circulating cold water pipeline 82 to cool down. The cooled water is transmitted to the constant temperature cold water storage tank 81 through the outlet of the semiconductor condenser 83. The cold water in the constant temperature cold water storage tank 81 enters the cold water tank 7 to realize the circulating cooling process. The circulating cold water pipeline 82 is a rubber tube.

[0058] In some embodiments of the present invention, the constant temperature cold water storage tank 81 is a multi-layer structure, which includes an inner tank, an insulation board, and an outer shell from the inside to the outside; the inner tank is made of stainless steel; the outer shell is made of stainless steel, and the insulation board is a polystyrene board.

[0059] In some embodiments of the present invention, Figure 1 As shown, a peristaltic pump 84 is provided on the circulating cold water pipeline 82 between the outlet of the constant temperature cold water storage tank 81 and the circulating cold water inlet of the cold water tank 7 to facilitate control of the flow rate. For example, the flow rate can be stabilized at 120 mL / min.

[0060] In some embodiments of the present invention, Figure 1 and Figure 3 As shown, the semiconductor condenser 83 includes a semiconductor heat exchange component, a temperature sensor and a controller. The temperature sensor is connected to the controller in communication, and the semiconductor heat exchange component is electrically connected to the controller. The temperature sensor is used to monitor the temperature of the water in the cold water tank 7 in real time and feed it back to the controller in time. The controller inputs a cooling instruction to the semiconductor heat exchange component. The semiconductor heat exchange component generates cold energy through the reverse voltage power supply of the controller, thereby quickly performing the cooling function. The controller is powered by low-voltage DC power.

[0061] In some embodiments of the present invention, Figure 1 As shown, the buffer stirring tank 1 is made of stainless steel, with a diameter of 20 cm, a height of 25 cm, an effective carrying capacity of 5 L, and a built-in electric stirring paddle, with a speed range of 0-600 rpm, specifically 300 rpm; the bacterial lysate collection tank 4 is made of 316L stainless steel, a hollow cylinder with an open top, a diameter of 25 cm, a height of 20 cm, and an effective carrying capacity of 5 L. The hot water tank 5 is 12 cm long, 12 cm wide, and 15 cm high. The cold water tank 7 is 12 cm long, 12 cm wide, and 15 cm high.

[0062] In some embodiments of the present invention, Figure 1 As shown, the heat transfer copper coil 2 is a hollow copper tube with an inner diameter of 2-3mm, specifically 2.5mm, an outer diameter of 4-5mm, specifically 4.5mm, and is arranged to have multiple turns, which are wound into a spiral cylinder. In this embodiment, 8 turns are arranged, and the diameter of each turn is 7-10cm, specifically 7cm; the cooling copper coil 3 is a hollow copper tube with an inner diameter of 2-3mm, specifically 2.5mm, an outer diameter of 4-5mm, specifically 4.5mm, and is arranged to have multiple turns, which are wound into a spiral cylinder. In this embodiment, 8 turns are arranged, and the diameter of each turn is 7-10cm, specifically 7cm.

[0063] Working principle: The resuspended bacteria liquid in the buffer stirring tank 1 is stirred by the built-in electric stirring paddle and then pumped to the heat transfer copper coil 2 by the peristaltic pump 1 9. The resuspended bacteria liquid in the heat transfer copper coil 2 exchanges heat with the hot water in the hot water tank 5. The temperature of the resuspended bacteria liquid increases and lysis occurs. At this time, the temperature of the circulating hot water entering the semiconductor heater 61 decreases. When the temperature sensor of the semiconductor heater 61 detects the temperature drop, it transmits a signal to the controller. The controller forwardly supplies power to generate heat to heat the circulating water. The heated water enters the constant temperature hot water storage tank 63. The hot water in the constant temperature hot water storage tank 63 is replenished to the hot water tank 5 via the peristaltic pump 2 64, thereby completing the circulation heat exchange process. The resuspended bacterial solution after thermal lysis is transported from the heat transfer copper coil 2 to the cooling copper coil 3 for cooling through a peristaltic pump 9. The bacterial lysate in the cooling copper coil 3 exchanges heat with the cold water in the cold water tank 7, and the temperature of the bacterial lysate decreases. At this time, the temperature of the circulating cold water entering the semiconductor condenser 83 increases. When the temperature sensor of the semiconductor condenser 83 detects the temperature increase, it transmits a signal to the controller. The controller reversely supplies power to generate cold energy to cool the circulating water. The cooled water enters the constant temperature cold water storage tank 81. The cooled water in the constant temperature cold water storage tank 81 is transported to the cold water tank 7 for replenishment through a peristaltic pump 3 84, thereby completing the circulating cooling process. The cooled bacterial lysate is pumped to the bacterial lysate collection tank 4 for collection.

[0064] Application Examples

[0065] In this application example, the buffer for resuspending the bacterial solution is STET buffer, which includes the following raw materials: 50 mM Tris, 100 mM ethylenediaminetetraacetic acid, 2% v / v Triton X-100, 8% w / v sucrose, and the pH of STET buffer is 8.2. Among them, Tris is trishydroxymethylaminomethane; Triton X-100 is polyethylene glycol octylphenyl ether.

[0066] The preparation method of the resuspended bacterial solution comprises the following steps: adding 2.8 mL of STET buffer per gram of wet bacteria to 50 g of bacterial cells, stirring and shaking sufficiently to make the bacterial cells resuspended evenly, adding human recombinant lysozyme with a final concentration of 200 U / mL, stirring and incubating at a constant temperature of 37°C at a speed of 20 rpm for 1 hour, and then immediately pouring into the buffer stirring tank 1.

[0067] Before the resuspended bacterial solution was lysed, the entire pipeline was first flushed with injection water, and then the entire pipeline was balanced with STET buffer. The volume of injection water and STET buffer was 3 times the volume of the resuspended bacterial solution, and the peristaltic pump flow rate was controlled at 120 mL / min.

[0068] The resuspended bacterial liquid in the buffer stirring tank 1 is pumped to the heat transfer copper coil 2 by a peristaltic pump 9, and heat exchange occurs with the hot water in the hot water tank 5 to cause lysis, and then it is transported to the cooling copper coil 3 for cooling. The cooled bacterial lysate is pumped to the bacterial lysate collection tank 4 for collection to obtain a lysate.

[0069] The lysate in the bacterial lysate collection tank 4 was centrifuged to obtain a supernatant. The centrifugation was performed using a high-speed refrigerated centrifuge. The parameters of the high-speed refrigerated centrifuge were set as follows: a centrifugal temperature of 4°C, a centrifugal speed of 12500 rpm, and a centrifugal time of 30 min. The supernatant was subjected to electrophoresis detection. The detection results were as follows: Figure 4 As shown, wherein, M1, DNA molecular weight marker B500352; 1, the first group of supernatant products after bacteria are lysed by the system of the utility model; 2, the second group of supernatant products after bacteria are lysed by the system of the utility model; 3, the third group of supernatant products after bacteria are lysed by the system of the utility model; 4, the standard product of the supernatant product obtained after purification; M2, DNA molecular weight marker B500352; and Figure 5 Compared with the electrophoresis diagram of , the yield of plasmid DNA after lysis of the utility model is higher, and the state is mainly supercoiled configuration, while open-circular and linear DNA account for a smaller proportion.

[0070] In summary, the utility model provides a circulating constant temperature system on the hot water tank 5 for accommodating the heat transfer copper coil 2 and the cold water tank 7 for accommodating the cooling copper coil 3, so as to shorten the temperature variation of the hot water tank 5 and the cold water tank 7 during the lysis process, thereby improving the lysis effect of the resuspended bacterial solution. Therefore, the utility model effectively overcomes various shortcomings in the prior art and has a high industrial utilization value.

[0071] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed in the present invention shall still be covered by the claims of the present invention.

Claims

1. A circulating heat exchange system for bacterial pyrolysis, characterized in that: The invention comprises a buffer stirring tank (1), a heat transfer copper coil (2), a cooling copper coil (3) and a bacterial lysate collection tank (4) which are fluidically connected in sequence; the heat transfer copper coil (2) is accommodated in a hot water tank (5) and immersed in hot water, and the hot water tank (5) is connected to a circulating hot water constant temperature system (6); the cooling copper coil (3) is accommodated in a cold water tank (7) and immersed in cold water, and the cold water tank (7) is connected to a circulating cold water constant temperature system (8).

2. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 1, characterized in that: The circulating hot water constant temperature system (6) comprises a constant temperature heat storage tank (63), a circulating hot water pipeline (62) and a semiconductor heater (61); the hot water tank (5) is provided with a circulating hot water inlet and a circulating hot water outlet; the circulating hot water outlet is connected to the inlet of the semiconductor heater (61) via the circulating hot water pipeline (62); the outlet of the semiconductor heater (61) is connected to the inlet of the constant temperature heat storage tank (63) via the circulating hot water pipeline (62); the outlet of the constant temperature heat storage tank (63) is connected to the circulating hot water inlet of the hot water tank (5) via the circulating hot water pipeline (62).

3. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 2, characterized in that: A second peristaltic pump (64) is provided on the circulating hot water pipeline (62) between the outlet of the constant temperature hot water storage tank (63) and the circulating hot water inlet of the hot water tank (5); And / or, the constant temperature hot water storage tank (63) is a multi-layer structure, which comprises, from inside to outside, an inner tank, a heat preservation plate, and an outer shell.

4. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 3, characterized in that: The semiconductor heater (61) comprises a semiconductor heat exchange component, a temperature sensor and a controller, wherein the temperature sensor is communicatively connected to the controller, and the semiconductor heat exchange component is electrically connected to the controller.

5. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 1, characterized in that: The circulating cold water constant temperature system (8) comprises a constant temperature cold water storage tank (81), a circulating cold water pipeline (82) and a semiconductor condenser (83); the cold water tank (7) is provided with a circulating cold water inlet and a circulating cold water outlet; the circulating cold water outlet is connected to the inlet of the semiconductor condenser (83) via the circulating cold water pipeline (82); the outlet of the semiconductor condenser (83) is connected to the inlet of the constant temperature cold water storage tank (81) via the circulating cold water pipeline (82); the outlet of the constant temperature cold water storage tank (81) is connected to the circulating cold water inlet of the cold water tank (7) via the circulating cold water pipeline (82).

6. A circulating heat exchange system for bacterial thermal lysis according to claim 5, characterized in that: A peristaltic pump three (84) is provided on the circulating cold water pipeline (82) between the outlet of the constant temperature cold water storage tank (81) and the circulating cold water inlet of the cold water tank (7); And / or, the constant temperature cold water storage tank (81) is a multi-layer structure, which comprises, from inside to outside, an inner tank, a heat preservation plate, and an outer shell.

7. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 5, characterized in that: The semiconductor condenser (83) comprises a semiconductor heat exchange component, a temperature sensor and a controller, wherein the temperature sensor is communicatively connected to the controller, and the semiconductor heat exchange component is electrically connected to the controller.

8. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 1, characterized in that: The buffer stirring tank (1) is equipped with an electric stirring paddle; And / or, the heat transfer copper coil (2) is a hollow copper tube, and the heat transfer copper coil (2) is arranged in multiple turns, and the diameter of each turn is 7-10 cm; And / or, the cooling copper coil (3) is a hollow copper tube, and the cooling copper coil (3) is arranged in multiple turns, and the diameter of each turn is 7-10 cm.

9. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 1, characterized in that: The inner diameter of the heat transfer copper coil (2) is 2-3 mm, and the outer diameter of the heat transfer copper coil (2) is 4-5 mm; And / or, the inner diameter of the cooling copper coil (3) is 2-3 mm, and the outer diameter of the cooling copper coil (3) is 4-5 mm.

10. A circulating heat exchange system for bacterial thermal pyrolysis according to claim 1, characterized in that: A peristaltic pump (9) is provided on a pipeline connecting the liquid outlet of the buffer solution stirring tank (1) and the liquid inlet of the heat transfer copper coil (2).

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