Irradiation structure based on ultrasonic transfection device

Abstract

The utility model discloses an irradiation structure based on an ultrasonic transfection device, which comprises a base, an ultrasonic transfection instrument main body and a box body are arranged above the base, an upper cover body is arranged above the box body, an air cylinder is arranged between the upper cover body and the box body, and a lower disc body for placing transfection test tubes is arranged in the box body. An upper disc body for limiting transfection test tubes is arranged below the upper cover body, a motor is arranged at the bottom end in the box body, and a transmission assembly is arranged between the motor and the lower disc body and the upper disc body. According to the ultrasonic transfection instrument, the upper cover body is descended to cover the box body, the upper disc body is also downwards pressed above the lower disc body and is used for limiting and fixing transfection test tubes above the lower disc body, the ultrasonic transfection instrument main body is used for carrying out ultrasonic irradiation on the interior of the box body, and meanwhile, the motor is started and drives the upper disc body and the lower disc body to rotate through the transmission assembly; the plurality of transfection test tubes continuously rotate to sequentially get close to and get away from the ultrasonic transfection instrument main body for ultrasonic irradiation.

Description

Technical Field

[0001] The utility model mainly relates to the technical field of ultrasonic transfection devices, in particular to an irradiation structure based on ultrasonic transfection devices. Background Art

[0002] Transfection is the process by which eukaryotic cells acquire new phenotypes by actively or passively introducing foreign DNA fragments. It is a commonly used method in gene therapy for diseases and gene research. Conventional transfection techniques can be divided into two categories: transient transfection and stable transfection (permanent transfection). Existing transfection methods mainly include electrofection, chemical transfection, and ultrasonic transfection. Electrofection and chemical transfection have high cytotoxicity. Ultrasonic targeted microbubble destruction technology in ultrasonic transfection can induce transient opening of the cell membrane, thereby increasing cell membrane permeability and delivering therapeutic substances to the disease site, becoming a safe, simple and effective delivery method.

[0003] Existing ultrasonic transfection devices usually place the test product inside a transfection test tube, and then place the transfection test tube inside a sterile box, and use an ultrasonic transfection instrument set on one side of the box to ultrasonically transfect the test product inside the test tube. Since the ultrasonic transfection instrument is set on one side of the box and the transfection test tube is set inside the box, the distance between them and the ultrasonic transfection instrument is different, and the test tube at the rear is also blocked by the test tube in the front, resulting in uneven ultrasonic irradiation of the internal test tube, which will affect the test results. Utility Model Content

[0004] The technical solution of the utility model aims at the technical problem that the existing technical solutions are too single, and provides a solution that is significantly different from the existing technology. It mainly provides an irradiation structure based on an ultrasonic transfection device to solve the technical problem that the ultrasonic transfection instrument proposed in the above background technology is set on one side of the box, because the distance between the test tubes inside the box and the ultrasonic transfection instrument is different, and there are test tubes in front of the test tubes at the back, which leads to uneven ultrasonic irradiation and affects the test results.

[0005] The technical solution adopted by the utility model to solve the above technical problems is:

[0006] An irradiation structure based on an ultrasonic transfection device comprises a base, an ultrasonic transfection instrument body and a box body are arranged above the base, an upper cover body is arranged above the box body, a cylinder is arranged between the upper cover body and the box body, a lower plate body for placing a transfection test tube is arranged inside the box body, an upper plate body for limiting the transfection test tube is arranged below the upper cover body, a motor is arranged at the bottom end of the box body, and a transmission assembly is arranged between the motor and the lower plate body and the upper plate body.

[0007] Preferably, the transmission assembly includes a rotating shaft, the rotating shaft is fixedly mounted on the output end of the motor, and the upper end of the rotating shaft is fixedly connected to the bottom of the lower disk.

[0008] Preferably, support blocks are fixedly installed on both sides of the box body, the support blocks are used to support the lower plate body, and the support blocks are slidably connected to the lower plate body.

[0009] Preferably, a circle of plug-in grooves is provided on the upper disk body, and the transfection test tubes are plugged into the plug-in grooves.

[0010] Preferably, a support rod is fixedly installed below the upper cover body, and the lower end of the support rod is rotatably connected to the upper disk body.

[0011] Preferably, a circle of fixing ring is provided on the upper disk body, the fixing ring is plugged into the plug-in groove, and the fixing ring fits the bottom plate of the transfection test tube.

[0012] Preferably, a plug-in rod is fixedly installed below the fixing ring, a plug-in hole is opened on the bottom plate of the transfection test tube, and the plug-in rod is plugged into the plug-in hole.

[0013] Compared with the prior art, the beneficial effects of the utility model are as follows: the upper cover body is lowered to cover the top of the box body, the upper disk body is also pressed down on the top of the lower disk body and restricts and fixes the transfection test tube above the lower disk body, and the inside of the box body is ultrasonically irradiated through the ultrasonic transfection instrument body, and the motor is started at the same time. The motor drives the upper disk body and the lower disk body to rotate through the transmission assembly, and multiple transfection test tubes are continuously rotated and approach and move away from the ultrasonic transfection instrument body in turn for ultrasonic irradiation, so that each test tube can be evenly irradiated with ultrasonic waves, reducing the error in the test results caused by uneven irradiation.

[0014] The present invention will be explained in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the front three-dimensional structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the front three-dimensional structure of the utility model in section;

[0017] Figure 3 This is a schematic diagram of the three-dimensional structure of the utility model in a cutaway view and viewed from above;

[0018] Figure 4 This is a schematic diagram of the internal structure of the upper cover of the utility model in the rising state;

[0019] Figure 5 This is a schematic diagram of the bottom structure of the upper cover body of the utility model;

[0020] Figure 6This is a schematic diagram of the three-dimensional structure of the transfection test tube of the utility model;

[0021] Description of reference numerals:

[0022] 1. Base; 2. Ultrasonic transfection instrument body; 3. Box body; 4. Upper cover body; 5. Lower plate body; 6. Motor; 7. Rotating shaft; 8. Support block; 9. Upper plate body; 10. Support rod; 11. Fixed ring; 12. Plug rod; 13. Transfection test tube; 14. Plug hole; 15. Cylinder. DETAILED DESCRIPTION

[0023] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings, but the present invention can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the content disclosed in the present invention more thorough and comprehensive.

[0024] It should be noted that when an element is referred to as being "fixed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly connected by technicians in the technical field of the present invention. The terminology used in the specification of the present invention is for the purpose of describing specific embodiments and is not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0026] Please refer to the attached Figure 1-Figure 6 An irradiation structure based on an ultrasonic transfection device includes a base 1, an ultrasonic transfection instrument body 2 and a box 3 are arranged above the base 1, an upper cover body 4 is arranged above the box 3, a cylinder 15 is arranged between the upper cover body 4 and the box 3, a lower plate body 5 for placing a transfection test tube 13 is arranged inside the box 3, an upper plate body 9 for limiting the transfection test tube 13 is arranged below the upper cover body 4, a motor 6 is arranged at the bottom end of the box 3, and a transmission assembly is arranged between the motor 6 and the lower plate body 5 and the upper plate body 9.

[0027] The specific operation process of the utility model is as follows: the transfection test tubes 13 are inserted into the plug-in slots provided on the lower disk body 5 one by one, and then the upper cover body 4 is lowered by the cylinder 15, and the upper cover body 4 is pressed down on the top of the box body 3, and the upper cover body 4 drives the upper disk body 9 to descend and cover the top of the lower disk body 5, and the upper disk body 9 restricts and fixes the multiple transfection test tubes 13 on the lower disk body 5, and then the ultrasonic head of the ultrasonic transfection instrument main body 2 is attached to one side of the box body 3, and the ultrasonic transfection instrument main body 2 is started, and the ultrasonic head is used to ultrasonically irradiate the transfection test tubes 13 inside the box body 3, and at the same time, the motor 6 is started, and the motor 6 drives the lower disk body 5 and the upper disk body 9 to rotate at the same time through the transmission component, so that the multiple transfection test tubes 13 are driven to rotate continuously, and each transfection test tube 13 is irradiated by ultrasonic wave in turn from near to far and from far to near, so that each transfection test tube 13 is evenly irradiated by ultrasonic wave, and the error of the test result is reduced.

[0028] Please refer to Figure 1-Figure 4 The transmission assembly includes a rotating shaft 7, which is fixedly installed at the output end of the motor 6. The upper end of the rotating shaft 7 is fixedly connected to the bottom of the lower disk body 5. Support blocks 8 are fixedly installed on both sides of the box body 3. The support blocks 8 are used to support the lower disk body 5, and the support blocks 8 are slidably connected to the lower disk body 5.

[0029] Start the motor 6, and the motor 6 drives the lower disk body 5 to rotate through the rotating shaft 7. The two sides of the lower disk body 5 are slidably connected with the support blocks 8. The support blocks 8 support and limit the lower disk body 5. A circle of sliding grooves is provided on the lower disk body 5. The support blocks 8 limit the lower disk body 5 by inserting the arc-shaped blocks into the sliding grooves. When the lower disk body 5 rotates, the arc-shaped blocks will slide along the sliding grooves.

[0030] Please refer to Figure 1-Figure 6 A circle of plug-in grooves is provided on the upper disk body 9, and the transfection test tube 13 is plugged into the plug-in grooves. A support rod 10 is fixedly installed under the upper cover body 4, and the lower end of the support rod 10 is rotatably connected to the upper disk body 9. A circle of fixing rings 11 is provided on the upper disk body 9, and the fixing ring 11 is plugged into the plug-in groove, and the fixing ring 11 fits the bottom plate of the transfection test tube 13. A plug-in rod 12 is fixedly installed under the fixing ring 11, and a plug-in hole 14 is provided on the bottom plate of the transfection test tube 13, and the plug-in rod 12 is plugged into the plug-in hole 14.

[0031] The upper cover body 4 descends, and the upper cover body 4 descends, driving the upper plate body 9 to descend and cover the lower plate body 5 through the support rod 10, and the fixing ring 11 is inserted into the plug-in slot. A circle of chassis is set at the bottom of the transfection test tube 13, and the fixing ring 11 is pressed down on the chassis. The fixing ring 11 is set as a circular ring with a hollow middle. The transfection test tube 13 will extend from the middle hole of the fixing ring 11, and the plug-in rods 12 on both sides of the fixing ring 11 will also be inserted into the plug-in hole 14 of the chassis. In this way, the transfection test tube 13 can be limited and fixed. When the lower plate body 5 rotates, the fixing ring 11 is pulled through the plug-in slot to drive the upper plate body 9 to rotate, and the rotation of the lower plate body 5 and the upper plate body 9 drives multiple transfection test tubes 13 to rotate.

[0032] The above description of the utility model in combination with the accompanying drawings is an exemplary description. Obviously, the specific implementation of the utility model is not limited to the above-mentioned method. As long as the non-substantial improvements are made by adopting the method concept and technical solution of the utility model, or the concept and technical solution of the utility model are directly applied to other occasions without improvement, they are all within the protection scope of the utility model.

Claims

1. An irradiation structure based on an ultrasonic transfection device, comprising a base (1), an ultrasonic transfection device body (2) and a box (3) are arranged above the base (1), an upper cover (4) is arranged above the box (3), and a cylinder (15) is arranged between the upper cover (4) and the box (3), characterized in that: A lower plate (5) for placing a transfection test tube (13) is arranged inside the box (3); an upper plate (9) for limiting the position of the transfection test tube (13) is arranged below the upper cover (4); a motor (6) is arranged at the bottom end of the box (3); and a transmission assembly is arranged between the motor (6), the lower plate (5) and the upper plate (9).

2. The irradiation structure based on the ultrasonic transfection device according to claim 1, characterized in that: The transmission assembly comprises a rotating shaft (7), the rotating shaft (7) being fixedly mounted on the output end of the motor (6), and the upper end of the rotating shaft (7) being fixedly connected to the bottom of the lower disk body (5).

3. The irradiation structure based on the ultrasonic transfection device according to claim 1, characterized in that: Support blocks (8) are fixedly installed on both sides of the box body (3), and the support blocks (8) are used to support the lower plate body (5), and the support blocks (8) are slidably connected to the lower plate body (5).

4. The irradiation structure based on the ultrasonic transfection device according to claim 1, characterized in that: The upper disk body (9) is provided with a circle of plug-in grooves, and the transfection test tube (13) is plugged into the plug-in grooves.

5. The irradiation structure based on the ultrasonic transfection device according to claim 4, characterized in that: A support rod (10) is fixedly installed below the upper cover body (4), and the lower end of the support rod (10) is rotatably connected to the upper disk body (9).

6. The irradiation structure based on the ultrasonic transfection device according to claim 5, characterized in that: The upper plate body (9) is provided with a circle of fixing rings (11), the fixing rings (11) are inserted into the insertion groove, and the fixing rings (11) are in close contact with the bottom plate of the transfection test tube (13).

7. The irradiation structure based on the ultrasonic transfection device according to claim 6, characterized in that: A plug-in rod (12) is fixedly mounted below the fixing ring (11), a plug-in hole (14) is provided on the bottom plate of the transfection test tube (13), and the plug-in rod (12) is plugged into the plug-in hole (14).

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