Cell culture device with material supplementing structure

Abstract

The utility model relates to the technical field of cell culture, and discloses a cell culture device with a feeding structure, which is characterized in that the side end of a shell is fixedly provided with an oxygen interface for conveying oxygen, the side end of the oxygen interface is fixedly provided with a carbon dioxide interface for conveying carbon dioxide, and one end of the shell is provided with a cover plate through a hinge; a control screen used for detection is fixedly installed at the center of the surface of the cover plate, one end of the control screen extends into the cover plate, the fixing assembly achieves stable fixation of the connecting rod and all assemblies on the connecting rod through cooperation of a spring, a ball and an expansion ring, the stability of the device is ensured, meanwhile, the assemblies can be replaced when needed, and the cost is reduced. Through the control of the rotating assembly, the placing base can move to the position corresponding to the lower spray head, the accurate material supplementing operation is realized, the stable fixing structure is beneficial to preventing the device from shaking or shifting in the rotating or material supplementing process, and the continuity and reliability of the culture process are ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device with a feed supplement structure. Background Art

[0002] In the field of cell biology research and application, cell culture is a crucial technology. As the core equipment of cell culture, the performance and quality of cell culture devices directly affect the efficiency and quality of cell growth. At present, there are many types of cell culture devices on the market, but they still have some shortcomings in function and design.

[0003] For example, a cell culture device proposed in a Chinese patent, with patent announcement number CN220201949U, realizes the culture and observation of cells through a specific structural design. When in use, the device can provide a relatively stable growth environment for cells and allow operators to observe and record cells outside the device. However, the above patent only solves the basic needs of cell culture and does not fully consider the problem of feeding in the cell culture process. During the long-term cell culture process, the nutrients in the culture medium will gradually be consumed. If they cannot be replenished in time, it will seriously affect the growth and reproduction of cells.

[0004] Some existing cell culture devices also have deficiencies in their fixing and supporting structures. Some devices use simple fixing methods, such as screws or clips, which are not only cumbersome to operate, but also easy to damage the device when replacing or adjusting components. At the same time, the fixing structure of some devices is not stable enough and is prone to shaking or shifting during rotation or feeding, thereby affecting the continuity and reliability of the culture process.

[0005] Therefore, in view of the shortcomings of existing cell culture devices, a cell culture device with a feeding structure is proposed. Through its unique design and working principle, this device realizes an efficient, stable, safe and easy-to-operate cell culture process, while solving problems such as feeding problems, insufficient fixing and supporting structures, and low levels of intelligence and automation, providing strong support for cell biology research and applications. Utility Model Content

[0006] 1. Technical issues to be resolved

[0007] In view of the deficiencies in the prior art, the utility model provides a cell culture device with a feeding structure to solve the above-mentioned problems.

[0008] (II) Technical solution

[0009] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture device with a feeding structure, comprising a shell, an oxygen interface for oxygen delivery is fixedly installed at the side end of the shell, a carbon dioxide interface for carbon dioxide delivery is fixedly installed at the side end of the oxygen interface, a cover plate is installed at one end of the shell through a hinge, a control screen for detection is fixedly installed at the center of the surface of the cover plate, one end of the control screen extends to the inside of the cover plate, and also includes:

[0010] A rotating assembly is installed inside the housing and is used to drive the structure for placing the base to move;

[0011] The fixing components are arranged at both ends of the inner part of the shell and are used to fix the connecting rod.

[0012] Preferably, the rotating assembly includes a main shaft, extension leaves and a placement base. The main shaft is movably installed at both ends of the inner wall of the outer shell, and a plurality of extension leaves are fixedly installed on the inner end surface of the main shaft. A rotating shaft is fixedly installed at the center of two corresponding extension leaves, and the surface of the rotating shaft is sleeved on the placement base.

[0013] Preferably, a plurality of empty slots are provided on the upper surface of the placement base, and the culture dishes are installed inside the empty slots.

[0014] Preferably, the fixing assembly includes a spring, a ball and an expansion ring, square grooves are provided at both ends of the shell, the inside of the square groove is fixedly connected to the spring, the other end of the spring is fixedly connected to the ball, an expansion ring is provided at the upper ends of the two balls, and the expansion ring is sleeved on both ends of the connecting rod.

[0015] Preferably, L-grooves are provided at both ends of the shell, and the fixing assembly is provided in the inner upper part of the L-grooves.

[0016] Preferably, a fixing piece is fixedly installed at the center of the connecting rod, a plurality of lower nozzles are fixedly installed on the lower end surface of the fixing piece, an atomization port is fixedly installed on the lower end of the lower nozzle, and a feed pipe is fixedly installed at the center of the upper end of the fixing piece, and the upper end of the feed pipe passes through the surface of the outer shell.

[0017] Preferably, a plurality of through holes are evenly arranged on the surface of the atomization port, and the plurality of atomization ports correspond to the positions of the plurality of culture dishes.

[0018] (III) Beneficial effects

[0019] Compared with the prior art, the utility model provides a cell culture device with a feeding structure, which has the following beneficial effects:

[0020] 1. The fixing assembly achieves a firm fixation of the connecting rod and all the components thereon through the cooperation of springs, balls and expansion rings, ensuring the stability of the device. At the same time, the components can be replaced when necessary. The feeding tube enables the operator to easily add new culture medium or other nutrients to the device without opening the entire device, thereby reducing the operational risks and the possibility of contamination. Through the control of the rotating assembly, the placement base can be moved to the corresponding position of the lower nozzle to achieve precise feeding operation. The stable fixing structure helps prevent the device from shaking or shifting during rotation or feeding, thereby ensuring the continuity and reliability of the culture process.

[0021] 2. Through the precise control of the rotating component, the cells in the culture dish can be evenly and continuously nourished by the culture medium, thereby accelerating the growth and reproduction of the cells. The rotating culture method helps the cells to be evenly distributed in the culture medium, avoiding the problems of cell accumulation and uneven nutrition, and further improving the culture efficiency. The control screen can monitor the situation inside the culture device in real time and automatically adjust the inside of the culture device. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the utility model;

[0023] Figure 2 It is the internal schematic diagram of the utility model;

[0024] Figure 3 It is a schematic diagram of the fixing component of the utility model.

[0025] In the figure: 1. Shell; 2. Cover; 3. Oxygen interface; 4. Carbon dioxide interface; 5. Control panel; 6. Main shaft; 7. Extension leaf; 8. Placement base; 9. Culture dish; 10. L-slot; 11. Connecting rod; 12. Atomization port; 13. Lower nozzle; 14. Fixing part; 15. Feeding tube; 16. Spring; 17. Ball; 18. Extension ring. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] See also Figure 1-3A cell culture device with a feeding structure comprises a shell 1, an oxygen interface 3 for oxygen delivery is fixedly installed at the side end of the shell 1, a carbon dioxide interface 4 for carbon dioxide delivery is fixedly installed at the side end of the oxygen interface 3, a cover plate 2 is installed at one end of the shell 1 through a hinge, a control screen 5 for detection is fixedly installed at the center of the surface of the cover plate 2, and one end of the control screen 5 extends to the inside of the cover plate 2, and also includes: a rotating component installed inside the shell 1, a structure for driving a placement base 8 to move; a fixing component, which is arranged at both ends of the inside of the shell 1, a structure for fixing a connecting rod 11, the feeding structure is replaced and fixed by the fixing component, and the position of a culture dish 9 is moved by the rotating component. When the position of the culture dish 9 moves to the lower end of the atomization port 12, the culture solution enters the inside of the culture dish 9 through the atomization port 12, and the multiple through holes on the surface of the atomization port 12 ensure that the culture solution can be evenly and finely sprayed into the inside of the culture dish 9.

[0028] Furthermore, the rotating assembly includes a main shaft 6, extension leaves 7 and a placement base 8. The main shaft 6 is movably installed at both ends of the inner wall of the outer shell 1. A plurality of extension leaves 7 are fixedly installed on the inner end surface of the main shaft 6. A rotating shaft is fixedly installed at the center of two corresponding extension leaves 7. The surface of the rotating shaft is sleeved on the placement base 8. The operation of the rotating assembly is controlled by the control panel 5.

[0029] Furthermore, a plurality of empty grooves are provided on the upper surface of the placement base 8 , and the culture dishes 9 are installed inside the empty grooves. The culture dishes 9 are evenly installed on the surface of the placement base 8 , and the installation position of the culture dishes 9 corresponds to the position of the atomization port 12 .

[0030] Furthermore, the fixing assembly includes a spring 16, a ball 17 and an expansion ring 18. Square grooves are set at both ends of the outer shell 1. The inside of the square groove is fixedly connected to the spring 16. The other end of the spring 16 is fixedly connected to the ball 17. An expansion ring 18 is set at the upper end of the two balls 17. The expansion ring 18 is sleeved on both ends of the connecting rod 11, forming the structure of the fixing assembly.

[0031] Furthermore, L grooves 10 are set at both ends of the shell 1, and the fixing components are set in the upper part of the inner part of the L groove 10. The fixing components are set at both ends of the inner part of the shell 1. The fixing components fix the feeding structure from both ends, and the L groove 10 is the movable range of the connecting rod 11.

[0032] Furthermore, a fixing part 14 is fixedly installed at the center of the connecting rod 11, a plurality of lower nozzles 13 are fixedly installed on the lower end surface of the fixing part 14, an atomizing port 12 is fixedly installed on the lower end of the lower nozzle 13, and a feeding pipe 15 is fixedly installed at the center of the upper end of the fixing part 14, the upper end of the feeding pipe 15 passes through the surface of the outer shell 1, forming a feeding structure.

[0033] Furthermore, a plurality of through holes are evenly arranged on the surface of the atomization port 12 , and the positions of the plurality of atomization ports 12 and the plurality of culture dishes 9 correspond to each other. The plurality of through holes on the surface of the atomization port 12 ensure that the culture solution can be sprayed evenly and finely into the interior of the culture dish 9 .

[0034] Working principle: the culture dish 9 is placed on the placement base 8 of the rotating component, and the base is connected to the housing 1 through the support of the main shaft 6 and the extension leaf 7. The main shaft 6 can be controlled to rotate through the control screen 5. When the main shaft 6 rotates, it drives the placement base 8 to move through the extension leaf, thereby rotating and culturing the cells inside the culture dish. When the fixing part 14 is installed, the expansion rings 18 at both ends of the connecting rod 11 enter the L groove 10, and the connecting rod 11 moves along the internal track of the L groove. When the expansion rings 18 at both ends of the connecting rod 11 pass through the fixing component, the connecting rod 11 squeezes the balls 17 on both sides, and the spring 16 is compressed. When the expansion ring 18 moves to the fixing component When the surface is touched, the spring 16 rebounds, and the ball 17 holds up and fixes the lower end of the expansion ring 18. At the same time, the feeding tube 15 at the upper end of the fixing part 14 passes through the circular groove on the outer shell 1 to be connected to the outside. However, when it is necessary to feed the cells inside the culture dish, the placement base 8 is controlled by the rotating component to move to the position corresponding to the lower nozzle 13, and the culture fluid enters the culture dish 9 through the atomization port 12. The multiple through holes on the surface of the atomization port 12 ensure that the culture fluid can be evenly and finely sprayed into the culture dish 9. The information inside the outer shell 1 can be observed through the control screen 5, and the use of the oxygen interface 3 and the carbon dioxide interface 4 can be controlled according to the internal situation to automatically adjust the inside of the outer shell 1.

[0035] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture device with a feeding structure, comprising a housing (1), an oxygen interface (3) for oxygen delivery fixedly mounted on a side end of the housing (1), a carbon dioxide interface (4) for carbon dioxide delivery fixedly mounted on a side end of the oxygen interface (3), a cover plate (2) fixedly mounted on one end of the housing (1) via a hinge, a control screen (5) for detection fixedly mounted at the center of the surface of the cover plate (2), one end of the control screen (5) extending to the interior of the cover plate (2), characterized in that: Also includes: A rotating assembly, installed inside the housing (1), and used to drive the structure for placing the base (8) to move; The fixing components are arranged at both ends of the interior of the housing (1) and are used to fix the connecting rod (11).

2. A cell culture device with a feed supplement structure according to claim 1, characterized in that: The rotating assembly comprises a main shaft (6), extension leaves (7) and a placement base (8); the main shaft (6) is movably mounted at both ends of the inner wall of the outer shell (1); a plurality of extension leaves (7) are fixedly mounted on the inner end surface of the main shaft (6); a rotating shaft is fixedly mounted at the center of two corresponding extension leaves (7); and the surface of the rotating shaft is sleeved with the placement base (8).

3. The cell culture device with a feed supplement structure according to claim 1, characterized in that: The upper surface of the placement base (8) is provided with a plurality of empty slots, and the culture dishes (9) are installed inside the empty slots.

4. The cell culture device with a feed supplement structure according to claim 1, characterized in that: The fixing assembly comprises a spring (16), a ball (17) and an expansion ring (18); square grooves are arranged at both ends of the housing (1); the interior of the square groove is fixedly connected to the spring (16); the other end of the spring (16) is fixedly connected to the ball (17); the expansion ring (18) is arranged at the upper ends of the two balls (17); and the expansion ring (18) is sleeved on both ends of the connecting rod (11).

5. The cell culture device with a feed supplement structure according to claim 1, characterized in that: L-grooves (10) are arranged at both ends of the housing (1), and the fixing assembly is arranged at the inner upper part of the L-grooves (10).

6. The cell culture device with a feed supplement structure according to claim 1, characterized in that: A fixing member (14) is fixedly mounted at the center of the connecting rod (11), a plurality of lower nozzles (13) are fixedly mounted on the lower end surface of the fixing member (14), an atomization port (12) is fixedly mounted at the lower end of the lower nozzle (13), and a feed pipe (15) is fixedly mounted at the center of the upper end of the fixing member (14), the upper end of the feed pipe (15) passing through the surface of the outer shell (1).

7. The cell culture device with a feed supplement structure according to claim 6, characterized in that: A plurality of through holes are evenly arranged on the surface of the atomization port (12), and the positions of the plurality of atomization ports (12) correspond to the positions of the plurality of culture dishes (9).

Images