Immunomagnetic bead enrichment device

Abstract

The immunomagnetic bead enrichment device comprises a reagent tube with an opening in the top, and the bottom of the reagent tube comprises a tube opening and a valve used for controlling the tube opening to be opened or closed; the bearing frame is used for bearing a reagent tube; the bearing frame comprises a top plate, a bottom plate located below the top plate and a supporting frame used for supporting the top plate and the bottom plate, and the reagent tube penetrates from the top of the top plate to the position below the bottom of the bottom plate; the magnetic attraction piece is located between the top plate and the bottom plate, is of a semi-surrounding structure and is vertically arranged, the magnetic attraction piece extends to the upper surface of the bottom plate from the lower surface of the top plate, and the magnetic attraction piece is matched with the outer side wall of the reagent tube. According to the device, the magnetic attraction part is attached to the outer side wall of the reagent tube, so that the attaching area of the magnetic attraction part and the outer wall of the reagent tube is increased, the enrichment efficiency is high, the structure is simple, waste liquid in the reagent tube and washing liquid after washing can be completely discharged through the tube opening valve below the reagent tube, immunomagnetic beads cannot be lost, and the enrichment efficiency of the immunomagnetic beads is improved.

Description

Technical Field

[0001] The present application relates to the technical field of immunomagnetic bead enrichment, and more specifically, to an immunomagnetic bead enrichment device. Background Art

[0002] Immunomagnetic bead technology is based on the specific binding of antigen and antibody and applies the principle of physical magnetic adsorption. It can quickly and effectively collect and concentrate small amounts of pathogenic microorganisms in large amounts of liquid samples.

[0003] At present, common immunomagnetic bead enrichment devices are magnetic racks or centrifugal devices. The magnetic rack is generally equipped with 1-3 small magnet blocks to enrich the immunomagnetic bead complex in the reagent tube. Due to the limited attraction of the small magnet blocks, the enrichment efficiency of the immunomagnetic beads is limited; when the centrifugal device enriches the immunomagnetic bead complex, when the centrifugal force is large, it will cause the antibodies in the immunomagnetic beads to separate from the immunomagnetic beads, thereby turning the immunomagnetic beads into naked magnetic beads, which is not conducive to the enrichment of the immunomagnetic beads. In addition, the above two devices require manual removal of waste liquid multiple times during the immunomagnetic bead enrichment process, which is time-consuming and labor-intensive. At the same time, a small amount of immunomagnetic beads may be mistakenly removed along with the waste liquid. Even if the waste liquid is removed multiple times, there will still be waste liquid residue at the bottom and wall of the tube, which will affect the enrichment effect of the immunomagnetic beads.

[0004] Therefore, in order to overcome the defects of the prior art, it is necessary to provide an immunomagnetic bead enrichment device. Utility Model Content

[0005] The purpose of the utility model is to provide an immunomagnetic bead enrichment device to solve at least one of the above technical problems.

[0006] In order to achieve at least one of the above purposes, the present application adopts the following technical solutions:

[0007] The present application provides an immunomagnetic bead enrichment device, comprising

[0008] A reagent tube with an open top, wherein the bottom of the reagent tube includes a tube opening and a valve for controlling the opening or closing of the tube opening;

[0009] A carrier for carrying the reagent tube; the carrier includes a top plate, a bottom plate located below the top plate, and a support frame for supporting the top plate and the bottom plate, the reagent tube passes through from the top of the top plate to the bottom of the bottom plate; the valve and the nozzle of the reagent tube are both located below the bottom plate;

[0010] A vertically arranged magnetic component in a semi-enclosed structure is located between the top plate and the bottom plate, and the magnetic component extends from the lower surface of the top plate to the upper surface of the bottom plate, and the magnetic component is adapted to the outer wall of the reagent tube.

[0011] Optionally, the top plate includes a receiving hole extending from its upper surface to its lower surface, and the reagent tube passes through the receiving hole from the top of the top plate to the bottom of the top plate.

[0012] Optionally, the top of the reagent tube extends outward to form an extension portion, and the extension portion is overlapped on the top plate.

[0013] Optionally, the support frame includes four support rods, which are respectively connected and fixed to four corners of the top plate and respectively penetrate the bottom plate to the bottom of the bottom plate.

[0014] Optionally, the reagent tube includes a body portion;

[0015] A material leakage portion is formed by extending downward from the main body portion, and the material leakage portion includes the pipe opening.

[0016] Optionally, the bottom plate further includes a through hole for the leakage portion of the reagent tube to pass through;

[0017] The pipe opening and the valve are located below the through hole.

[0018] Optionally, the through hole extends through and passes through a side wall of the bottom plate.

[0019] Optionally, the reagent tube is made of plastic.

[0020] The beneficial effects of this application are as follows:

[0021] In response to the problems existing in the current prior art, the present application provides an immunomagnetic bead enrichment device, which allows a liquid sample to fall into the inner cavity of a reagent tube through an opening at the top of the reagent tube, and under the action of the magnetic attraction of the magnetic suction component, the immunomagnetic bead complex in the liquid sample is enriched on the inner wall of the reagent tube on the side where the magnetic suction component is attached, and the valve at the bottom of the tube mouth of the reagent tube is opened to discharge the waste liquid; since the immunomagnetic bead complex enriched on the inner wall of the reagent tube is also doped with other impurities, in order to remove the impurities, the immunomagnetic bead complex needs to be washed after the waste liquid is discharged. Specifically, a washing solution is poured into the reagent tube, and the washing solution can be a phosphate buffered saline solution. After washing is completed, the valve is opened to discharge the washing solution. Generally, the washing needs to be repeated two to three times to finally obtain the desired immunomagnetic bead complex. The magnetic suction piece is arranged between the top plate and the bottom plate, and forms a whole with the top plate and the bottom plate. The vertical arrangement can better adapt to the outer wall of the reagent tube, that is, increase the corresponding area with the outer wall of the reagent tube, and improve the enrichment efficiency of the immunomagnetic bead complex in the liquid sample in the reagent tube; the support frame is used to support the top plate and the bottom plate, and the reagent tube can be set up to a certain height, so that the waste liquid in the reagent tube can be discharged through the nozzle. The immunomagnetic bead enrichment device provided by the present application increases the corresponding area of ​​the magnetic suction piece and the outer wall of the reagent tube by cooperating with the magnetic suction piece in a semi-enclosed structure with the outer wall of the reagent tube, so that the immunomagnetic bead complex in the liquid sample can be gathered together, and the enrichment efficiency is high, and the top plate, the bottom plate and the magnetic suction piece are a whole, which not only simplifies the structure of the whole device, but also the reagent tube can be replaced at will, and will not be affected by the magnetic suction piece; the setting of the nozzle valve below the reagent tube can discharge all the waste liquid in the reagent tube and the washing liquid after washing, and will not cause the immunomagnetic beads to be lost, nor will the immunomagnetic beads become bare magnetic beads due to problems such as centrifugation, which greatly improves the enrichment efficiency of the immunomagnetic beads. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings.

[0023] Figure 1 A schematic diagram showing the overall structure of an immunomagnetic bead enrichment device in one embodiment of the present application is shown. DETAILED DESCRIPTION

[0024] In the following description, for the purpose of explanation, in order to provide a thorough understanding of one or more embodiments, numerous specific details are set forth. However, it is apparent that these embodiments may also be implemented without these specific details.

[0025] In the description of the present application, it should be noted that the terms "upper", "lower", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. Unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0026] It should also be noted that, in the description of the present application, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or equipment. In the absence of further restrictions, the elements defined by the statement "comprise one..." do not exclude the presence of other identical elements in the process, method, article or equipment including the elements.

[0027] In order to solve the problems existing in the prior art, an embodiment of the present application provides an immunomagnetic bead enrichment device, such as Figure 1 As shown, it includes a reagent tube with an open top, the material of the reagent tube is plastic, and the specific material can be acrylic, which has high transparency and is convenient for staff to observe the enrichment of immunomagnetic beads; the bottom of the reagent tube includes a nozzle (not shown in the figure), and a valve 2 for controlling the opening or closing of the nozzle; a support frame for carrying the reagent tube; the support frame includes a top plate 3, a bottom plate 5 located below the top plate, and a support frame for supporting the top plate 3 and the bottom plate 5, and the reagent tube runs from the top of the top plate 3 to the bottom of the bottom plate 5; the nozzles of the valve 2 and the reagent tube are both located below the bottom plate 5; a vertically arranged magnetic suction member in a semi-enclosed structure between the top plate and the bottom plate, the magnetic suction member extends from the lower surface of the top plate to the upper surface of the bottom plate, and the magnetic suction member is adapted to the outer wall of the reagent tube. Specifically, the magnetic suction member 6 is connected to the top plate 3 and the bottom plate 5 to form a whole, and the cross section of the magnetic suction member 6 is arc-shaped, and preferably, its arc is not less than 180°.

[0028] In the above-mentioned embodiment of the present application, the liquid sample is dropped into the inner cavity of the reagent tube through the opening at the top of the reagent tube, and the immunomagnetic bead complex in the liquid sample is enriched on the inner wall of the reagent tube on the side where the magnetic suction component 6 is attached under the action of the magnetic suction force of the magnetic suction component 6. The valve 2 at the bottom of the tube mouth of the reagent tube is opened to discharge the waste liquid; since the immunomagnetic bead complex enriched on the inner wall of the reagent tube is also doped with other impurities, in order to remove the impurities, the immunomagnetic bead complex needs to be washed after the waste liquid is discharged. Specifically, a washing solution is poured into the reagent tube, and the washing solution can be a phosphate buffered saline solution. After washing, the valve 2 is opened to discharge the washing solution. Generally, the washing needs to be repeated two to three times to finally obtain the desired immunomagnetic bead complex. The magnetic attraction component 6 is arranged between the top plate 3 and the bottom plate 5, forming a whole with the top plate 3 and the bottom plate 5. The vertical arrangement can better adapt to the outer wall of the reagent tube, that is, increase the corresponding area with the outer wall of the reagent tube, and improve the enrichment efficiency of the immunomagnetic bead complex in the liquid sample in the reagent tube; the support frame is used to support the top plate 3 and the bottom plate 5, and can enable the reagent tube to be erected to a certain height, so as to facilitate the discharge of waste liquid in the reagent tube through the tube mouth. The immunomagnetic bead enrichment device provided in the present application increases the corresponding area between the magnetic element 6 and the outer wall of the reagent tube by matching the magnetic element 6 with a semi-enclosed structure with the outer wall of the reagent tube, so that the immunomagnetic bead complex in the liquid sample can be gathered together, and the enrichment efficiency is high. In addition, the top plate 3, the bottom plate 5 and the magnetic element 6 are a whole, which not only simplifies the structure of the entire device, but also allows the reagent tube to be replaced at will without being affected by the magnetic element 6. The setting of the nozzle valve 2 under the reagent tube can discharge all the waste liquid in the reagent tube and the washing liquid after washing, without causing the loss of the immunomagnetic beads, and the immunomagnetic beads will not become naked magnetic beads due to problems such as centrifugation, thereby greatly improving the enrichment efficiency of the immunomagnetic beads.

[0029] Specifically, when discharging waste liquid, a collection container for receiving waste liquid can be placed under the pipe opening, and after the valve 2 is opened, the waste liquid in the reagent tube is discharged into the collection container through the pipe opening.

[0030] In a specific embodiment, the top plate 3 includes a receiving hole (not shown in the figure) extending from its upper surface to its lower surface, and the reagent tube passes through the receiving hole from the top of the top plate 3 to the bottom of the top plate 3. Specifically, after the reagent tube passes through the receiving hole to the bottom of the top plate 3, it moves downward until the tube mouth passes through the bottom plate 5 to the bottom of the bottom plate 5. The design of the receiving hole allows the reagent tube to be mounted on the top plate 3, so that the reagent tube is in a vertical state, and the degree of fit with the magnetic suction component 6 is higher, and it is also convenient to drop the liquid sample into the reagent tube and discharge the waste liquid. In addition, the inner diameter of the receiving hole is slightly larger than the outer diameter of the side wall of the reagent tube. The reagent tube can slide relative to the side wall of the receiving hole, which is convenient for detaching from the top plate 3, that is, it is convenient to replace the reagent tube, so that the enrichment of immunomagnetic beads in different liquid samples can be achieved.

[0031] Specifically, the top of the reagent tube extends outward to form an extension portion 11, and the extension portion 11 overlaps the top plate 3. Specifically, the top opening of the reagent tube is a liquid inlet 14, and the extension portion 11 is formed by extending outward from the outer circumference of the liquid inlet 14; the extension portion 11 overlaps the outside of the accommodating hole, that is, overlaps the top plate 3, and the upper surface of the top plate 3 supports the extension portion 11, which further improves the stability of the reagent tube.

[0032] In a specific embodiment, the support frame includes four support rods 4, which are respectively connected and fixed to the four corners of the top plate 3 and respectively penetrate the bottom plate 5 to the bottom of the bottom plate 5. It is convenient to fix it on the ground or other test platforms. Here, the support rods 4 are also fixedly connected to the bottom plate 5, that is, the four support rods 4, the top plate 3 and the bottom plate 5 are a whole, which improves the stability of the whole device.

[0033] In a specific embodiment, the reagent tube includes a main body 12, a leakage portion 13 extending downward from the main body 12, and the leakage portion 13 includes the tube opening. The leakage portion 13 is funnel-shaped, and when the waste liquid is discharged from the reagent tube, no waste liquid will remain in the reagent tube.

[0034] Furthermore, the bottom plate 5 also includes a through hole 51 for the leakage part 13 of the reagent tube to pass through; the tube mouth is located below the through hole 51. Specifically, the reagent tube passes through the accommodating hole and the through hole 51 on the top plate 3 in sequence until the tube mouth is located below the bottom plate 5. In practical applications, the collection container can be placed below the tube mouth, that is, below the bottom plate 5, without any obstruction, so that the waste liquid in the reagent tube can be discharged into the collection container.

[0035] Furthermore, the through hole 51 extends and passes through a side wall of the bottom plate 5. This is equivalent to having an observation hole on the bottom plate 5. When the waste liquid in the reagent tube is discharged, the staff can see the discharge of the waste liquid into the collection container, and can also see the opening and closing of the valve 2 through the observation hole; in addition, when replacing the reagent tube, it is also convenient for the valve 2 to pass through, and it will not block the valve, nor affect the replacement of the reagent tube.

[0036] In a specific embodiment, the reagent tube may have multiple, such as Figure 1 As shown, there may be two of them, which are respectively arranged on the supporting frame, and multiple liquid samples can be enriched with immunomagnetic beads at the same time to improve the enrichment efficiency.

[0037] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made based on the above description. It is impossible to list all the implementation methods here. All obvious changes or modifications derived from the technical solution of the present invention are still within the scope of protection of the present invention.

Claims

1. An immunomagnetic bead enrichment device, characterized in that: include A reagent tube with an open top, wherein the bottom of the reagent tube includes a tube opening and a valve for controlling the opening or closing of the tube opening; A carrier for carrying the reagent tube; the carrier includes a top plate, a bottom plate located below the top plate, and a support frame for supporting the top plate and the bottom plate, the reagent tube passes through from the top of the top plate to the bottom of the bottom plate; the valve and the nozzle of the reagent tube are both located below the bottom plate; A vertically arranged magnetic component in a semi-enclosed structure is located between the top plate and the bottom plate, and the magnetic component extends from the lower surface of the top plate to the upper surface of the bottom plate, and the magnetic component is adapted to the outer wall of the reagent tube.

2. The immunomagnetic bead enrichment device according to claim 1, characterized in that: The top plate includes a receiving hole extending from its upper surface to its lower surface, and the reagent tube passes through the receiving hole from the top of the top plate to the bottom of the top plate.

3. The immunomagnetic bead enrichment device according to claim 1, characterized in that: The top of the reagent tube extends outward to form an extension portion, and the extension portion is overlapped on the top plate.

4. The immunomagnetic bead enrichment device according to claim 1, characterized in that: The support frame includes four support rods, which are respectively connected and fixed to four corners of the top plate and respectively penetrate the bottom plate to the bottom of the bottom plate.

5. The immunomagnetic bead enrichment device according to claim 1, characterized in that: The reagent tube includes a body portion; A material leakage portion is formed by extending downward from the main body portion, and the material leakage portion includes the pipe opening.

6. The immunomagnetic bead enrichment device according to claim 5, characterized in that: The bottom plate also includes a through hole for the leakage portion of the reagent tube to pass through; The pipe opening and the valve are located below the through hole.

7. The immunomagnetic bead enrichment device according to claim 6, characterized in that: The through hole extends and passes through a side wall of the bottom plate.

8. The immunomagnetic bead enrichment device according to claim 1, characterized in that: The reagent tube is made of plastic.

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