Rotary disc device for protein crystal growth
By introducing temperature regulation and limiting components into the protein crystal growth device, the problem that the rotating disc device is difficult to maintain a constant temperature is solved, precise control and optimization of protein crystal growth is achieved, and the growth quality and research of protein crystals are improved. Controlled.
Patent Information
- Application Number
- CN202421150891.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-24
- Publication Date
- 2025-01-10
- Estimated Expiration
- 2034-05-24
AI Technical Summary
Existing rotary disc devices for protein crystal growth are difficult to maintain a constant crystal growth temperature, affecting the quality and growth conditions of protein crystals.
A device including a rotating disc body, a protein crystal sample pool limiting assembly and a temperature regulation assembly is designed to achieve precise temperature regulation through medium circulation pipes, heaters, refrigerators and motor-driven driving driving wheels. Rapid exchange with protein solutions ensures that protein crystals grow under optimal conditions.
It realizes precise control of the growth temperature of protein crystals, prevents displacement, optimizes the arrangement of protein molecules and crystal nuclei formation, and improves the growth quality and research controllability of protein crystals.
Smart Images
Figure CN222324288U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of protein crystal growth, in particular to a rotating disc device used for protein crystal growth. Background Art
[0002] In the fields of biochemistry and structural biology, the growth and structure elucidation of protein crystals are crucial to understanding the functions and mechanisms of proteins.
[0003] Since the growth of protein crystals is very sensitive to temperature, during the crystallization process, changes in temperature will cause changes in the diffusion rate of protein molecules in the solution, thereby affecting the growth rate of the crystals. Generally speaking, the growth rate of crystals increases with increasing temperature. However, if the temperature changes too much, it may cause the protein molecules to aggregate too quickly, resulting in a decrease in crystal quality.
[0004] In actual use, most of the existing rotating disk devices for protein crystal growth are not convenient for maintaining a constant crystal growth temperature and ensuring that protein crystals grow under optimal conditions. Therefore, how to design a rotating disk device for protein crystal growth has become a problem that we currently need to solve. Utility Model Content
[0005] 1. Technical issues to be resolved
[0006] In view of the deficiencies of the prior art, the utility model provides a rotating disc device for growing protein crystals, which solves the problems mentioned in the above background technology.
[0007] (II) Technical solution
[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a rotating disk device for growing protein crystals, comprising a rotating disk body, and a protein crystal sample pool limiting component and a temperature regulating component located inside the rotating disk body.
[0009] The temperature regulating component includes a medium circulation pipeline, which is fixedly installed inside the bottom of the rotating disc body, a circulation pump is fixedly installed at the end of the medium circulation pipeline, a heater is installed on one side of the circulation pump, a refrigerator is fixedly installed on one side of the heater, and a temperature conduction plate is fixedly installed on the top of the rotating disc body.
[0010] Preferably, the protein crystal sample pool limiting assembly includes a first motor, which is fixedly mounted inside the top of the rotating disc body, and a bidirectional screw is fixedly mounted on the output end of the first motor, and two groups of guide assembly seats are threadedly connected on the surface of the bidirectional screw, and limiting blocks are fixedly mounted above the two groups of guide assembly seats.
[0011] Preferably, a guide groove is provided on the surface of the rotating disc body.
[0012] Preferably, a disc base is rotatably mounted on the bottom of the rotating disc body.
[0013] Preferably, a second motor is fixedly installed inside the disc base.
[0014] Preferably, the output end of the second motor is fixedly connected to a driving wheel, and a driven wheel is meshed on one side of the driving wheel.
[0015] Preferably, the driven wheel is fixedly connected to the rotating disc body.
[0016] Preferably, a protein crystal sample pool body is placed above the rotating disc body, a sample pool cover is engaged with the top of the protein crystal sample pool body, a liquid injection port is provided on one side of the sample pool cover, a buffer storage compartment is elastically installed inside the sample pool cover through a first spring, a push button is fixedly installed on the top of the buffer storage compartment, a liquid outlet is provided at the bottom center of the buffer storage compartment, a second spring is connected below the liquid outlet, a rubber plug is fixedly connected to the other end of the second spring, and a plug is installed below the rubber plug.
[0017] (III) Beneficial effects
[0018] The utility model provides a rotating disc device for growing protein crystals, which has the following
[0019] Beneficial effects:
[0020] First, by setting up a protein crystal sample pool limit assembly, the first motor is started to drive the bidirectional screw to rotate, thereby driving the guide assembly seat and the limit block to move inward in the horizontal direction of the guide groove to complete the clamping and fixation of the protein crystal sample pool. This setting facilitates the precise positioning of the protein crystal sample pool on the rotating disk body, which can conveniently observe and study the growth process of protein crystals, and at the same time prevents the protein crystal sample pool from being displaced when the rotating disk body rotates.
[0021] Secondly, by setting up a temperature regulating component, when the temperature around the rotating disk body exceeds or is lower than the constant crystal growth temperature during use, the refrigerator or heater can be started by controlling the cooling or heating of the medium inside the medium circulation pipeline, and circulated to the medium circulation pipeline through the circulation pump, so that the temperature around the rotating disk body is lowered or increased. This setting improves the problem that most of the existing rotating disks used for protein crystal growth are not convenient for maintaining a constant crystal growth temperature and ensuring that protein crystals grow under optimal conditions.
[0022] Third: by starting the second motor to drive the driving wheel to rotate, and then drive the driven wheel and the rotating disk body to rotate, this setting can make the protein solution in the protein crystal sample pool quickly exchanged, which is conducive to the orderly arrangement of protein molecules and the formation of crystal nuclei. By adjusting the speed of the second motor, it can help study the growth of protein crystals under centrifugal fields of different sizes, and then optimize the growth conditions of protein crystals. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 This is a schematic diagram of the main structure of the utility model;
[0024] Figure 2 This is a schematic diagram of the internal structure of the disc base of the utility model;
[0025] Figure 3 This is a schematic diagram of the structure of the limit assembly of the utility model;
[0026] Figure 4 This is a schematic diagram of the structure of the temperature regulating component of the utility model;
[0027] Figure 5 It is a schematic diagram of the internal structure of the sample pool cover of the utility model.
[0028] In the figure: 1. rotating disc body; 2. disc base; 3. driving wheel; 4. protein crystal sample pool limit assembly; 401. guide groove; 402. limit block; 403. bidirectional screw rod; 404. guide assembly seat; 405. first motor; 5. temperature adjustment assembly; 501. temperature conduction plate; 502. refrigerator; 503. heater; 504. circulation pump; 505. medium circulation pipeline; 6. second motor; 7. driven wheel; 8. pressing key; 9. sample pool cover; 10. protein crystal sample pool body; 11. first spring; 12. rubber plug; 13. second spring; 14. plug rod; 15. liquid injection port; 16. buffer storage compartment. DETAILED DESCRIPTION
[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0030] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0031] In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, "multiple" means two or more, unless otherwise clearly and specifically defined.
[0032] like Figure 1-Figure 5 As shown, the utility model provides a technical solution: a rotating disc device for growing protein crystals, comprising a rotating disc body 1, and a protein crystal sample pool limiting component 4 and a temperature regulating component 5 located inside the rotating disc body 1, a disc base 2 is rotatably mounted on the bottom of the rotating disc body 1, a second motor 6 is fixedly mounted inside the disc base 2, a driving wheel 3 is fixedly connected to the output end of the second motor 6, a driven wheel 7 is meshed on one side of the driving wheel 3, the driven wheel 7 is fixedly connected to the rotating disc body 1, and the upper portion of the rotating disc body 1 is fixedly mounted on the rotating disc body 1. A protein crystal sample pool body 10 is placed on the side, a sample pool cover 9 is engaged with the top of the protein crystal sample pool body 10, a liquid injection port 15 is provided on one side of the sample pool cover 9, a buffer storage compartment 16 is elastically installed inside the sample pool cover 9 through a first spring 11, a push button 8 is fixedly installed on the top of the buffer storage compartment 16, a liquid outlet is provided at the bottom center of the buffer storage compartment 16, a second spring 13 is connected below the liquid outlet, a rubber plug 12 is fixedly connected to the other end of the second spring 13, and a plug 14 is installed below the rubber plug 12.
[0033] By starting the second motor 6 to drive the driving wheel 3 to rotate, and then drive the driven wheel 7 and the rotating disc body 1 to rotate, this setting can make the protein solution in the protein crystal sample pool quickly exchanged, which is conducive to the orderly arrangement of protein molecules and the formation of crystal nuclei. By adjusting the rotation speed of the second motor 6, it can help study the growth of protein crystals under centrifugal fields of different sizes, and then optimize the growth conditions of protein crystals; through the sample pool cover 9, when it is necessary to add buffer to the protein crystal sample pool body 10, there is no need to open the sample pool cover 9, only need to press the push button 8 to drive the buffer storage compartment 16 to move downward, When the rubber stopper 12 under the buffer storage chamber 16 hits the insertion rod 14, it will retract backwards, so that the buffer solution flows into the protein crystal sample pool body 10 through the liquid outlet. This arrangement solves the problem that some existing protein crystal sample pool bodies 10 usually need to inject buffer solution into the protein crystal sample pool body 10 when in use to ensure that the protein crystallizes within the optimal pH range, and the sample pool cover 9 needs to be opened when pouring the buffer solution, which easily causes the protein crystal sample pool body 10 to be exposed to the air for a long time, resulting in the protein in the protein crystal sample pool body 10 being easily contaminated by exogenous microorganisms, resulting in the crystallization being affected.
[0034] Specifically, the temperature adjustment component 5 includes a medium circulation pipeline 505, which is fixedly installed inside the bottom of the rotating disc body 1, a circulation pump 504 is fixedly installed at the end of the medium circulation pipeline 505, a heater 503 is installed on one side of the circulation pump 504, a refrigerator 502 is fixedly installed on one side of the heater 503, and a temperature conduction plate 501 is fixedly installed on the top of the rotating disc body 1. When the ambient temperature of the rotating disc body 1 exceeds or is lower than the constant crystal growth temperature, the refrigerator 502 or the heater 503 can be controlled to start, the medium inside the medium circulation pipeline 505 can be cooled or heated, and circulated to the medium circulation pipeline 505 through the circulation pump 504, so that the ambient temperature of the rotating disc body 1 decreases or increases.
[0035] Specifically, the protein crystal sample pool limiting assembly 4 includes a first motor 405, which is fixedly mounted inside the top of the rotating disc body 1, and a bidirectional screw 403 is fixedly mounted on the output end of the first motor 405, and two groups of guide assembly seats 404 are threadedly connected on the surface of the bidirectional screw 403, and a limiting block 402 is fixedly mounted above the two groups of guide assembly seats 404, and a guide groove 401 is provided on the surface of the rotating disc body 1. The first motor 405 is started to drive the bidirectional screw 403 to rotate, and then drive the guide assembly seat 404 and the limiting block 402 to move inward in the horizontal direction of the guide groove 401, so as to complete the clamping and fixing of the protein crystal sample pool. This arrangement facilitates the accurate positioning of the protein crystal sample pool on the rotating disc body 1, and can conveniently observe and study the growth process of the protein crystal, while preventing the protein crystal sample pool from being displaced when the rotating disc body 1 rotates.
[0036] The working process of the utility model is as follows: when the rotating disc device for growing protein crystals is used, the protein crystal sample pool can be placed on the surface of the rotating disc body 1, and the first motor 405 is started to drive the bidirectional screw 403 to rotate through the protein crystal sample pool limiting component 4, thereby driving the guide assembly seat 404 and the limiting block 402 to move inward in the horizontal direction of the guide groove 401 to complete the clamping and fixing of the protein crystal sample pool. This arrangement facilitates the accurate positioning of the protein crystal sample pool on the rotating disc body 1, and can conveniently observe and study the growth process of the protein crystal, while preventing the protein crystal sample pool from being displaced when the rotating disc body 1 rotates; after the positioning is completed, the second motor 6 is started to drive the driving wheel 3 to rotate, thereby driving the driven wheel 7 and the rotating disc body 1 to rotate. This arrangement can make the protein crystal sample pool The protein solution in the rotating disk body 1 is quickly exchanged, which is conducive to the orderly arrangement of protein molecules and the formation of crystal nuclei. By adjusting the rotation speed of the second motor 6, it can help study the growth of protein crystals under centrifugal fields of different sizes, and then optimize the growth conditions of protein crystals. Through the set temperature regulating component 5, when the temperature around the rotating disk body 1 exceeds or falls below the constant crystal growth temperature during use, the refrigerator 502 or the heater 503 can be controlled to start, and the medium inside the medium circulation pipeline 505 can be cooled or heated, and circulated to the medium circulation pipeline 505 through the circulation pump 504, so that the temperature around the rotating disk body 1 decreases or increases. This setting improves the problem that most of the existing rotating disks used for protein crystal growth are not convenient for maintaining a constant crystal growth temperature and ensuring that protein crystals grow under optimal conditions.
[0037] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A rotating disk device for growing protein crystals, characterized in that: It comprises a rotating disc body (1), and a protein crystal sample pool limiting component (4) and a temperature regulating component (5) located inside the rotating disc body (1); The temperature regulating component (5) comprises a medium circulation pipeline (505), wherein the medium circulation pipeline (505) is fixedly installed inside the bottom of the rotating disc body (1), a circulation pump (504) is fixedly installed at the end of the medium circulation pipeline (505), a heater (503) is fixedly installed on one side of the circulation pump (504), a refrigerator (502) is fixedly installed on one side of the heater (503), and a temperature conduction plate (501) is fixedly installed on the top of the rotating disc body (1).
2. A rotating disk device for growing protein crystals according to claim 1, characterized in that: The protein crystal sample pool limiting assembly (4) comprises a first motor (405), the first motor (405) is fixedly mounted inside the top of the rotating disc body (1), a bidirectional screw rod (403) is fixedly mounted on the output end of the first motor (405), two groups of guide assembly seats (404) are threadedly connected on the surface of the bidirectional screw rod (403), and limiting blocks (402) are fixedly mounted above the two groups of guide assembly seats (404).
3. A rotating disk device for growing protein crystals according to claim 1, characterized in that: A guide groove (401) is provided on the surface of the rotating disc body (1).
4. A rotating disk device for growing protein crystals according to claim 1, characterized in that: A disc base (2) is rotatably mounted on the bottom of the rotating disc body (1).
5. A rotating disk device for growing protein crystals according to claim 4, characterized in that: A second motor (6) is fixedly mounted inside the disc base (2).
6. A rotating disk device for growing protein crystals according to claim 5, characterized in that: The output end of the second motor (6) is fixedly connected to a driving wheel (3), and a driven wheel (7) is meshed on one side of the driving wheel (3).
7. A rotating disk device for growing protein crystals according to claim 6, characterized in that: The driven wheel (7) is fixedly connected to the rotating disc body (1).
8. A rotating disk device for growing protein crystals according to claim 1, characterized in that: A protein crystal sample pool body (10) is placed above the rotating disc body (1), a sample pool cover (9) is engaged with the top of the protein crystal sample pool body (10), a liquid injection port (15) is provided on one side of the sample pool cover (9), a buffer storage compartment (16) is elastically mounted inside the sample pool cover (9) via a first spring (11), a push button (8) is fixedly mounted on the top of the buffer storage compartment (16), a liquid outlet is provided at the bottom center of the buffer storage compartment (16), a second spring (13) is connected below the liquid outlet, a rubber plug (12) is fixedly connected to the other end of the second spring (13), and a plug rod (14) is mounted below the rubber plug (12).