High-efficiency peripheral blood mononuclear cell recycling and filtering centrifugal tube

Abstract

The utility model discloses a high-efficiency peripheral blood mononuclear cell recovering and filtering centrifuge tube which comprises a centrifuge tube, a sliding mechanism fixedly sleeved on the centrifuge tube, a sealing sleeve and a fixing component arranged on the sliding mechanism, a filtering tube arranged in the centrifuge tube in a sliding manner, a connecting ring arranged on the filtering tube, and the connecting ring connected with the sliding mechanism, when the sliding mechanism slides, the filter tube is pushed to slide out of the centrifugal tube, and the sliding mechanism is fixed by the fixing assembly after the gap between the opening of the centrifugal tube and the filter tube is sealed by the sealing sleeve. The filter tube is inserted into the centrifugal tube, the filter screen is mounted on the filter tube through threads, a solution can be filtered through the filter screen, the sliding mechanism and the sealing sleeve are mounted outside the centrifugal tube, and the sealing sleeve is pushed by the sliding mechanism to seal a gap between the centrifugal tube and the filter tube; and negative pressure is formed in the sealed centrifugal tube by drawing out the filter tube, so that the filtration of a solution in the filter tube is accelerated, and the filtration speed is increased.

Description

Technical Field

[0001] The utility model belongs to the technical field of filtration separation, and in particular relates to a high-efficiency peripheral blood mononuclear cell recovery filtration centrifuge tube. Background Art

[0002] Peripheral blood mononuclear cells (PBMC) are a mixed cell population of cells with a single nucleus in peripheral blood (i.e., blood other than bone marrow), including natural killer cells (NK), T lymphocytes (70%-90%), and B lymphocytes. They can be further separated and purified and are the main source of immune cells. Therefore, it is necessary to collect peripheral blood mononuclear cells when diagnosing some diseases.

[0003] At present, some complex technologies, such as mass spectrometry technology, are difficult to detect in time or require centralized testing of specimens. To meet this demand, it is necessary to extract peripheral blood mononuclear cells. Currently, the market mostly uses Percoll density gradient centrifugation technology for white blood cell separation, but the cell recovery rate is low.

[0004] Therefore, we need to design a high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube to solve these problems. Utility Model Content

[0005] The problem to be solved by the utility model is to provide a high-efficiency peripheral blood mononuclear cell recovery and filtering centrifuge tube. This scheme intends to design a high-efficiency cell recovery centrifuge tube, which collects white blood cells on the filter through filtering and then elutes them, thereby improving the disadvantage of loss of peripheral blood white blood cell separation in density gradient centrifugation.

[0006] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0007] A high-efficiency peripheral blood mononuclear cell recovery and filtering centrifuge tube comprises a centrifuge tube, a sliding mechanism is fixedly mounted on the centrifuge tube, a sealing sleeve and a fixing assembly are arranged on the sliding mechanism, a filter tube is slidingly arranged in the centrifuge tube, a connecting ring is arranged on the filter tube, and the connecting ring is connected to the sliding mechanism. When the sliding mechanism slides, the filter tube is pushed to slide out of the centrifuge tube, and after the sealing sleeve seals the gap between the centrifuge tube opening and the filter tube, the sliding mechanism will be fixed by the fixing assembly.

[0008] Preferably, the sliding mechanism includes an inner sleeve assembly and an outer sleeve assembly, the inner sleeve assembly includes an inner sleeve body, a push ring and a limit ring which are sleeved on the centrifuge tube, a plurality of slide grooves are axially arranged on the outer wall of the inner sleeve body, a support groove is arranged in the slide groove, a limit hole is arranged at the end of the inner sleeve body opposite to the limit ring, a limit rod is also fixedly arranged on the limit ring, the free end of the limit rod extends into the limit hole, and a limit block is also fixedly arranged on the free end of the limit rod, the push ring is slidably arranged on the limit ring and the limit rod on the inner sleeve body, the sealing sleeve is arranged between the push ring and the limit ring, a support rod is also fixedly arranged on the push ring, the support rod is located in the slide groove, and a hook is fixedly arranged on the free end of the support rod.

[0009] Furthermore, the outer sleeve assembly includes an outer sleeve body slidably mounted on the inner sleeve body, a plurality of guide grooves are arranged on the inner wall of the outer sleeve body, the guide grooves are opened along the axial direction of the outer sleeve body, a clamping groove is arranged in the guide groove, the clamping groove matches the clamping hook, and a plurality of connecting blocks are also fixedly arranged on the outer sleeve.

[0010] With such arrangement, the inner sleeve body and the outer sleeve body can seal the gap between the centrifuge tube and the filter tube while the centrifuge tube and the filter tube slide relative to each other, so that negative pressure is formed in the centrifuge tube, and the filtering speed is accelerated by the air pressure difference.

[0011] Preferably, the fixing assembly includes a fixing block arranged on the inner sleeve body, and also includes a fixing groove opened on the outer sleeve, and a reset block is arranged on the fixing groove.

[0012] With such arrangement, the outer sleeve body can be fixed after the outer sleeve body slides to the limit position, thereby fixing the filter tube and maintaining a negative pressure state in the centrifuge tube.

[0013] Preferably, the connecting ring is fixedly sleeved on the outer wall of one end of the filter tube, and a plurality of connecting grooves are opened on the connecting ring, and the connecting grooves correspond to the positions of the plurality of connecting blocks one by one and match each other. A filter screen is arranged at the other end of the filter tube, and the filter screen is connected to the filter tube by a threaded seal.

[0014] With such arrangement, the connection ring and the outer sleeve body can be connected through the connection groove on the connection ring and the connection block at the end of the outer sleeve body, so that the filter tube can be installed in the centrifuge tube.

[0015] Preferably, the gap between the centrifuge tube and the filter tube does not exceed 0.5 mm, the length of the filter tube does not exceed the length of the centrifuge tube, and is not less than the sliding length of the sliding mechanism.

[0016] This arrangement avoids the gap between the centrifuge tube and the filter tube being too large, which makes it difficult to complete the sealing, and also avoids the filter tube having a diameter much smaller than the centrifuge tube, which results in a poor negative pressure effect after the sealing is completed.

[0017] Preferably, a silicone sleeve is fitted on the inner side of the inner sleeve body and the inner side of the connecting ring, and the inner diameter of the silicone sleeve on the inner wall of the inner sleeve body is smaller than the outer diameter of the centrifuge tube, the inner diameter of the silicone sleeve on the inner side of the connecting ring is smaller than the outer diameter of the filter tube, and the inner side of the limiting ring is trumpet-shaped.

[0018] Such arrangement enables the inner sleeve body to be interference fit on the centrifuge tube through the silicone sleeve, the connecting ring to be interference fit on the filter tube, and the trumpet-shaped limiting ring on the inner side to improve the sealing effect of the sealing sleeve on the joint between the centrifuge tube opening and the filter tube.

[0019] The advantages and positive effects of the utility model are:

[0020] The utility model inserts a filter tube into a centrifuge tube, and a filter screen is installed on the filter tube through threads, so that the solution can be filtered through the filter screen. At the same time, a sliding mechanism and a sealing sleeve are installed outside the centrifuge tube, and the sliding mechanism is used to push the sealing sleeve to seal the gap between the centrifuge tube and the filter tube. The filter tube is drawn outward to form a negative pressure in the sealed centrifuge tube, thereby accelerating the filtration of the solution in the filter tube, and can effectively improve the filtration speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0022] Figure 1 This is an axonometric schematic diagram of the centrifuge tube and filter tube assembly structure of the utility model;

[0023] Figure 2 It is a schematic diagram of the installation structure of the inner sleeve, the outer sleeve and the centrifugal tube of the utility model;

[0024] Figure 3 It is a schematic diagram of the connection slot position structure of the utility model;

[0025] Figure 4 It is a schematic diagram of the structure of the inner sleeve assembly of the utility model;

[0026] Figure 5It is a schematic diagram of the cross-sectional structure of the inner sleeve assembly of the utility model;

[0027] Figure 6 This is a schematic diagram of the cross-sectional structure of the outer sleeve of the utility model;

[0028] Figure 7 This is a cross-sectional schematic diagram of the use state of the utility model

[0029] Figure 8 yes Figure 7 A magnified view of the structure in Figure 2.

[0030] The following are the descriptions of the reference numerals:

[0031] 1. Centrifuge tube; 2. Filter tube; 3. Filter screen; 4. Outer sleeve body; 5. Guide groove; 6. Clamping groove; 7. Inner sleeve body; 8. Push ring; 9. Support rod; 10. Hook; 11. Limiting ring; 12. Sealing sleeve; 13. Support groove; 14. Limiting rod; 15. Limiting hole; 16. Limiting block; 17. Connecting ring; 18. Connecting block; 19. Connecting groove; 20. Fixed block; 21. Fixed groove; 22. Reset block; 23. Slide groove. DETAILED DESCRIPTION

[0032] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0033] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0034] The utility model is further described below in conjunction with the accompanying drawings:

[0035] Example 1: Figure 1-Figure 8 As shown, a high-efficiency peripheral blood mononuclear cell recovery and filtering centrifuge tube comprises a centrifuge tube 1, a sliding mechanism is fixedly mounted on the centrifuge tube 1, a sealing sleeve 12 and a fixing component are arranged on the sliding mechanism, a filter tube 2 is slidingly arranged in the centrifuge tube 1, a connecting ring 17 is arranged on the filter tube 2, and the connecting ring 17 is connected to the sliding mechanism. When the sliding mechanism slides, it will push the filter tube 2 to slide out of the centrifuge tube 1, and after the sealing sleeve 12 seals the gap between the opening of the centrifuge tube 1 and the filter tube 2, the sliding mechanism will be fixed by the fixing component.

[0036] like Figure 2 , Figure 4 and Figure 5 As shown, the sliding mechanism includes an inner sleeve assembly and an outer sleeve assembly, the inner sleeve assembly includes an inner sleeve body 7, a push ring 8 and a limit ring 11 which are sleeved on the centrifuge tube 1, a plurality of slide grooves 23 are axially arranged on the outer wall of the inner sleeve body 7, a support groove 13 is arranged in the slide groove 23, a limit hole 15 is arranged at the end of the inner sleeve body 7 opposite to the limit ring 11, a limit rod 14 is also fixedly arranged on the limit ring 11, the free end of the limit rod 14 extends into the limit hole 15, and a limit block 16 is also fixedly arranged on the free end of the limit rod 14, the push ring 8 is slidably arranged on the limit ring 11 and the limit rod 14 on the inner sleeve body 7, the sealing sleeve 12 is arranged between the push ring 8 and the limit ring 11, and a support rod 9 is also fixedly arranged on the push ring 8, the support rod 9 is located in the slide groove 23, and a hook 10 is fixedly arranged on the free end of the support rod 9.

[0037] like Figure 2 and Figure 6 As shown, the outer sleeve assembly includes an outer sleeve body 4 which is slidably mounted on the inner sleeve body 7, and a plurality of guide grooves 5 are arranged on the inner wall of the outer sleeve body 4, the guide grooves 5 are opened along the axial direction of the outer sleeve body 4, and a clamping groove 6 is arranged in the guide groove 5, and the clamping groove 6 matches the clamping hook 10. A plurality of connecting blocks 18 are also fixedly arranged on the outer sleeve, so that the inner sleeve body 7 and the outer sleeve body 4 can seal the gap between the centrifuge tube 1 and the filter tube 2 while the centrifuge tube 1 and the filter tube 2 slide relative to each other, so that a negative pressure is formed in the centrifuge tube 1, and the filtering speed is accelerated by the air pressure difference.

[0038] like Figure 1 , Figure 4 , Figure 5 and Figure 6As shown, the fixing assembly includes a fixing block 20 arranged on the inner sleeve body 7, and also includes a fixing groove 21 opened on the outer sleeve, and a reset block 22 is arranged on the fixing groove 21. Such a setting can fix the outer sleeve body 4 after the outer sleeve body 4 slides to the extreme position, thereby achieving the fixation of the filter tube 2, so that the centrifuge tube 1 can maintain a negative pressure state.

[0039] like Figure 3 and Figure 7 As shown, the connecting ring 17 is fixedly sleeved on the outer wall of one end of the filter tube 2, and a plurality of connecting grooves 19 are opened on the connecting ring 17. The positions of the connecting grooves 19 and the plurality of connecting blocks 18 correspond to each other and match each other. A filter screen 3 is arranged at the other end of the filter tube 2, and the filter screen 3 is connected to the filter tube 2 through a threaded seal. In this way, the connection between the connecting ring 17 and the outer sleeve body 4 can be realized through the connecting groove 19 on the connecting ring 17 and the connecting block 18 at the end of the outer sleeve body 4, so that the filter tube 2 can be installed in the centrifuge tube 1.

[0040] like Figure 7 As shown, the gap between the centrifuge tube 1 and the filter tube 2 does not exceed 0.5 mm, the length of the filter tube 2 does not exceed the length of the centrifuge tube 1, and is not less than the sliding length of the sliding mechanism. This arrangement avoids the gap between the centrifuge tube 1 and the filter tube 2 being too large, making it difficult to complete the sealing, and at the same time avoids the filter tube 2 having a diameter much smaller than the centrifuge tube 1, resulting in a poor negative pressure effect after the sealing is completed.

[0041] Specifically, the inner side of the inner sleeve body 7 and the inner side of the connecting ring 17 are both fitted with a silicone sleeve, and the inner diameter of the silicone sleeve on the inner wall of the inner sleeve body 7 is smaller than the outer diameter of the centrifuge tube 1, and the inner diameter of the silicone sleeve inside the connecting ring 17 is smaller than the outer diameter of the filter tube 2. The inner side of the limiting ring 11 is trumpet-shaped. This arrangement enables the inner sleeve body 7 to be fitted on the centrifuge tube 1 through the silicone sleeve, and the connecting ring 17 can be fitted on the filter tube 2. The trumpet-shaped inner side of the limiting ring 11 can improve the sealing effect of the sealing sleeve 12 on the joint between the opening of the centrifuge tube 1 and the filter tube 2. .

[0042] The working process of this embodiment is as follows: before use, first install the filter screen 3 capable of filtering leukocytes on the filter tube 2, then insert the filter tube 2 into the centrifuge tube 1, then connect the connecting ring 17 with the outer sleeve body 4 through the connecting groove 19 on the connecting ring 17 and the connecting block 18 on the outer sleeve body 4, then pull the filter tube 2 upward through the connecting ring 17, because at this time the hook 10 on the support rod 9 is stuck in the groove 6 in the guide groove 5, so when the connecting ring 17 drives the filter tube 2 to move upward, it will push the push ring 8 upward through the support rod 9, and when the push ring 8 moves upward, it will push the limit ring 11 upward through the sealing sleeve 12, and when the limit ring 11 moves upward, The limiting rod 14 will be drawn out from the limiting hole 15, and the limiting ring 11 will stop moving when the limiting block 16 on the limiting rod 14 contacts the inner wall of the limiting hole 15. At this time, the outer sleeve body 4 will drive the push ring 8 to move upward through the cooperation of the card slot 6 and the hook 10. The push ring 8 will squeeze the sealing sleeve 12 into the limiting ring 11 during the process of continuing to move upward. Since the inner wall of the limiting ring 11 is trumpet-shaped, the sealing sleeve 12 will be squeezed to the joint between the opening of the centrifuge tube 1 and the filter tube 2 to seal the centrifuge tube 1. Then, as the outer sleeve body 4 continues to move upward, the push ring 8 will be blocked by the sealing sleeve 12, so the hook 10 will be stuck in the card slot 6. The filter tube 2 is pushed out, and at the same time, the support rod 9 will undergo elastic deformation, so that the end of the support rod 9 enters the support groove 13, and at the same time, the outer sleeve body 4 will continue to drive the filter tube 2 to move upward until the fixing block 20 is stuck in the fixing groove 21. At this time, the outer sleeve body 4 will be fixed, so that the filter tube 2 will not be affected by the negative pressure in the centrifuge tube 1 and slide toward the centrifuge tube 1. At the same time, since the centrifuge tube 1 is in a negative pressure state at this time, the solution in the filter tube 2 will penetrate through the filter screen 3 into the centrifuge tube 1 under the action of the external atmospheric pressure. After the filtration is completed, the fixing block 20 can be pushed out of the fixing groove 21 by pressing the reset block 22. At this time, due to the filter The reagent in tube 2 has been filtered, so the air pressure in centrifuge tube 1 is the same as the air pressure outside centrifuge tube 1. Therefore, the outer sleeve body 4 is pushed downward in the opposite direction. When the hook 10 slides to the corresponding slot 6, the hook 10 will bounce into the slot 6. Therefore, when the outer sleeve body 4 moves downward, the push ring 8 will be driven downward through the support rod 9 and the limit ring 11 will be driven downward through the sealing sleeve 12 until it is reset. Then the connecting ring 17 is removed from the outer sleeve body 4, the filter tube 2 is pulled out of the centrifuge tube 1, and the filter screen 3 is removed from the filter tube 2 and the filter screen is cleaned, completing the separation of white blood cells in the filtrate in the centrifuge tube 1.

[0043] In this embodiment, since the substance filtered through the filter tube 2 is white blood cells or other biological samples, and the volume of the biological samples is relatively small, the mesh size of the filter 3 will be correspondingly larger, and the water permeability of the filter 3 with a larger mesh size will become larger. At the same time, since the viscosity of the solution is also greater than that of water, the traditional filtration method using gravity has a slow filtration speed. Therefore, in this embodiment, the filtration speed is accelerated by changing the air pressure, thereby improving the filtration efficiency.

[0044] The above is a detailed description of an embodiment of the utility model, but the content is only a preferred embodiment of the utility model and cannot be considered to limit the scope of implementation of the utility model. All equivalent changes and improvements made within the scope of application of the utility model should still fall within the scope of the patent coverage of the utility model.

Claims

1. A high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube, comprising a centrifuge tube (1), characterized in that: A sliding mechanism is fixedly mounted on the centrifuge tube (1), a sealing sleeve (12) and a fixing assembly are arranged on the sliding mechanism, a filter tube (2) is slidingly mounted in the centrifuge tube (1), a connecting ring (17) is arranged on the filter tube (2), and the connecting ring (17) is connected to the sliding mechanism. When the sliding mechanism slides, it pushes the filter tube (2) to slide out of the centrifuge tube (1), and after the sealing sleeve (12) seals the gap between the opening of the centrifuge tube (1) and the filter tube (2), the sliding mechanism is fixed by the fixing assembly.

2. The high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube according to claim 1, characterized in that: The sliding mechanism comprises an inner sleeve assembly and an outer sleeve assembly, wherein the inner sleeve assembly comprises an inner sleeve body (7), a push ring (8) and a limit ring (11) which are sleeved on the centrifuge tube (1), a plurality of slide grooves (23) are axially arranged on the outer wall of the inner sleeve body (7), a support groove (13) is arranged in the slide groove (23), a limit hole (15) is arranged at the end of the inner sleeve body (7) opposite to the limit ring (11), and a limit rod (14) is fixedly arranged on the limit ring (11), and the limit rod (14) is The free end extends into the limiting hole (15), and a limiting block (16) is fixedly arranged on the free end of the limiting rod (14), the push ring (8) is slidably arranged on the limiting ring (11) and the limiting rod (14) on the inner sleeve body (7), the sealing sleeve (12) is arranged between the push ring (8) and the limiting ring (11), and a support rod (9) is fixedly arranged on the push ring (8), the support rod (9) is located in the slide groove (23), and a hook (10) is fixedly arranged on the free end of the support rod (9).

3. The high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube according to claim 2, characterized in that: The outer sleeve assembly comprises an outer sleeve body (4) which is slidably mounted on the inner sleeve body (7). A plurality of guide grooves (5) are arranged on the inner wall of the outer sleeve body (4). The guide grooves (5) are opened along the axial direction of the outer sleeve body (4). A clamping groove (6) is arranged in the guide groove (5). The clamping groove (6) matches the clamping hook (10). A plurality of connecting blocks (18) are also fixedly arranged on the outer sleeve.

4. The high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube according to claim 3, characterized in that: The fixing assembly comprises a fixing block (20) arranged on the inner sleeve body (7), and also comprises a fixing groove (21) opened on the outer sleeve, wherein a reset block (22) is arranged on the fixing groove (21).

5. The high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube according to claim 3, characterized in that: The connecting ring (17) is fixedly sleeved on the outer wall of one end of the filter tube (2), and a plurality of connecting grooves (19) are provided on the connecting ring (17). The connecting grooves (19) correspond to the positions of the plurality of connecting blocks (18) one by one and match each other. A filter screen (3) is provided at the other end of the filter tube (2), and the filter screen (3) is connected to the filter tube (2) in a threaded sealing manner.

6. The high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube according to claim 1, characterized in that: The gap between the centrifuge tube (1) and the filter tube (2) does not exceed 0.5 mm, the length of the filter tube (2) does not exceed the length of the centrifuge tube (1), and is not less than the sliding length of the sliding mechanism.

7. The high-efficiency peripheral blood mononuclear cell recovery and filtration centrifuge tube according to claim 2, characterized in that: The inner side of the inner sleeve body (7) and the inner side of the connecting ring (17) are both fitted with a silicone sleeve, and the inner diameter of the silicone sleeve on the inner wall of the inner sleeve body (7) is smaller than the outer diameter of the centrifuge tube (1), the inner diameter of the silicone sleeve on the inner side of the connecting ring (17) is smaller than the outer diameter of the filter tube (2), and the inner side of the limiting ring (11) is trumpet-shaped.

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