Cell filtering and collecting bag

Abstract

The utility model discloses a cell filtering and collecting bag. A cell filtering and collecting bag comprises a collecting bag body, a feeding pipe is installed at the top of the collecting bag body, a discharging pipe is installed at the bottom end of the collecting bag body, a filtering net is installed in the collecting bag body, an air pipe is installed on the collecting bag body, a microfiltration filter element is installed on the air pipe, and a microfiltration filter element is installed on the microfiltration filter element. A first control valve and a second control valve are respectively mounted on the feeding pipe and the discharging pipe. According to the cell filtering and collecting bag provided by the utility model, the collecting bag body, the feed pipe, the filter screen, the discharge pipe, the first control valve, the second control valve, the air pipe and the microfiltration filter element are arranged, so that continuous and closed operation of a cell suspension can be realized, and the microbial pollution risk of the cell suspension is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell collection, in particular to a cell filtering and collecting bag. Background Art

[0002] Bioreactor is a very important equipment in the field of bioengineering. It can use biochemical reactions in organisms to produce chemicals, drugs, enzymes and other biological products.

[0003] When collecting the cell suspension obtained from the bioreactor, if it is not filtered, larger cell clumps will enter the downstream process, such as continuous flow centrifuge equipment. Filtration generally requires filtering the cell suspension into the centrifuge bottle through a cell filter installed at the mouth of the centrifuge bottle under open conditions in a biosafety cabinet. It is difficult to achieve continuous and closed operation, and the risk of microbial contamination is high.

[0004] Therefore, it is necessary to provide a cell filtration collection bag to solve the above technical problems. Utility Model Content

[0005] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a cell filtration collection bag capable of reducing the risk of microbial contamination of the cell suspension.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] The cell filtration collection bag comprises: a collection bag body, a feed pipe installed on the top of the collection bag body, and a discharge pipe installed on the bottom. The feed pipe and the discharge pipe are thermoplastic tubes. A filter screen is installed near the bottom of the collection bag body. An air pipe is also installed on the collection bag body. A microfiltration filter element for sterilization is installed on the air pipe. A first control valve and a second control valve are installed on the feed pipe and the discharge pipe respectively.

[0008] Preferably, the pore size of the filter is 40-100 μm.

[0009] Preferably, the filter screen is a flat plate type.

[0010] Preferably, the filter screen is in a cone barrel shape.

[0011] Preferably, a first mounting frame is installed at the bottom of the filter screen, the first mounting frame is connected to the collecting bag body, a support rod is installed on the first mounting frame, and the support rod is connected to the top of the filter screen.

[0012] Preferably, a guide cone is installed at the end of the filter screen.

[0013] Preferably, a second mounting frame is installed at the end of the filter screen, and the second mounting frame is connected to the support rod.

[0014] Compared with the prior art, the utility model has the following beneficial effects:

[0015] (1) The utility model can realize continuous and closed operation of cell suspension by arranging a collection bag body, a feed pipe, a filter screen, a discharge pipe, a first control valve, a second control valve, an air pipe and a microfiltration filter element, thereby reducing the risk of microbial contamination of the cell suspension;

[0016] (2) The utility model can increase the service life of the filter screen by providing a cone-shaped filter screen, thereby extending the service life of the device;

[0017] (3) The utility model can prevent cell agglomerates from accumulating on the top of the filter by installing a guide cone at the end of the filter;

[0018] (4) The utility model can improve the stability of the filter when in use by installing a second mounting frame at the end of the filter. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the structure of the cell filtration collection bag provided by the utility model;

[0020] Figure 2 for Figure 1 A schematic diagram of the structure of an embodiment of a filter screen in a cell filtration collection bag is shown;

[0021] Figure 3 for Figure 1 A schematic diagram of the structure of an embodiment of a filter screen in a cell filtration collection bag is shown;

[0022] Figure 4 for Figure 1 Schematic diagram of installation of filter screen in cell filtration collection bag shown;

[0023] Figure 5 for Figure 1 Schematic diagram of the installation of the filter in the cell filtration collection bag is shown.

[0024] Among them, the names corresponding to the figure marks are: 1-collecting bag body, 2-feed pipe, 3-filter screen, 4-discharge pipe, 5-first control valve, 6-second control valve, 7-air pipe, 8-microfiltration filter element, 9-first mounting bracket, 10-support rod, 11-guide cone, 12-second mounting bracket. DETAILED DESCRIPTION

[0025] The present invention is further described below in conjunction with the accompanying drawings and embodiments. The present invention includes but is not limited to the following embodiments.

[0026] Embodiment 1:

[0027] like Figure 1-5 As shown, the cell filtration collection bag provided by the utility model comprises: a collection bag body 1, which can be in various shapes such as a rounded cube, a cylinder, a sphere, a bag-shaped body, etc. to adapt to different working scenes, and its specifications range from 1L to 50L to adapt to different scales of cell collection needs, a feed pipe 2 is installed on the top of the collection bag body 1, and a discharge pipe 4 is installed on the bottom, the feed pipe 2 and the discharge pipe 4 are thermoplastic pipes, and a filter screen 3 is installed near the bottom of the collection bag body 1, and the pore size of the filter screen 3 is 40-10 0μm, and its diameter can be designed to be various specifications ranging from 30mm to 150mm according to the capacity of the collection bag body 1. An air pipe 7 is also installed on the collection bag body 1, and a 0.22μm microfiltration filter element 8 for sterilization is installed on the air pipe 7. The 0.22μm microfiltration filter element 8 is mainly used in the sterilization filter. The first control valve 5 and the second control valve 6 are respectively installed on the feed pipe 2 and the discharge pipe 4. When in use, the feed pipe 2 is connected to the bioreactor, and the discharge pipe 4 is connected to the centrifugal device. The first control valve 5 and the second control valve 6 is opened, and the cell suspension in the bioreactor is discharged from the feed pipe 2 into the collecting bag body 1 for temporary storage, and the excess air in the collecting bag body 1 is discharged out of the bag through the microfiltration filter element 8, and then the larger cell agglomerates in the cell suspension are intercepted by the filter mesh 3, and the rest will pass through the filter mesh 3 and be discharged into the centrifugal device through the discharge pipe 4 for the next process, thereby realizing the continuous aseptic operation of biological cell preparation and reducing the risk of microbial contamination. The above is the first use method. Another use method is as follows: the feed pipe 2 is connected to the bioreactor, the first control valve 5 is opened, and the second control valve 6 is closed, the cell suspension in the bioreactor can be discharged into the collecting bag body 1 for storage, and then the first control valve 5 is closed to realize the collection of the cell suspension, and then the feed pipe 2 is removed from the bioreactor, the device is moved above the centrifugal bottle mouth, the discharge pipe 4 is aligned with the bottle mouth, and the second control valve 6 is opened, and the cell suspension in the bag is filtered through the filter mesh 3 and discharged into the centrifugal bottle, and the external air is sterilized and filtered through the microfiltration filter element 8 and then enters the collecting bag body 1.

[0028] By arranging the collecting bag body 1, the feed pipe 2, the filter screen 3, the discharge pipe 4, the first control valve 5, the second control valve 6, the air pipe 7 and the microfiltration filter element 8, continuous and closed operation of the cell suspension can be achieved, thereby reducing the risk of microbial contamination of the cell suspension.

[0029] Embodiment 2:

[0030] like Figure 1 As shown, the filter screen 3 is a flat plate type, and the four sides of the filter screen 3 are sealed and connected to the inner wall of the collection bag body 1. When in use, the cell suspension in the collection bag body 1 is filtered through the flat plate filter screen 3. When all the meshes of the filter screen 3 are blocked, filtering cannot continue and needs to be replaced.

[0031] By providing a flat filter screen 3, filtering of the cell suspension can be achieved.

[0032] Embodiment 3:

[0033] like Figure 2 and Figure 4-5 As shown, the filter screen 3 is in a cone barrel shape, and a first mounting frame 9 is installed at the bottom of the filter screen 3. The first mounting frame 9 is fixedly connected to the inner wall of the collection bag body 1, and a support rod 10 is installed at the center of the first mounting frame 9. The support rod 10 is connected and fixed to the top center of the filter screen 3 to support the filter screen 3 to prevent it from being damaged due to excessive deformation. When in use, Figure 4-5 As shown, the filter 3 is installed with the tip facing upward or downward, and the cell suspension in the collection bag body 1 is filtered by the filter 3. Since the shape of the filter 3 has a larger filtering area, the service life of the filter 3 can be increased, and when the larger cell agglomerates in the cell suspension encounter the filter 3, they will slide down along the inclined surface of the filter 3, so that the cell agglomerates are automatically concentrated, avoiding the cell agglomerates from being spread on the surface of the filter 3, thereby effectively extending the service life of the filter 3.

[0034] By providing the cone-barrel-shaped filter screen 3, the service life of the filter screen 3 can be increased, thereby extending the service life of the device.

[0035] Embodiment 4:

[0036] like Figure 2 As shown, a guide cone 11 is installed at the end of the filter screen 3. When in use, the filter screen 3 is installed with the tip facing upward, and the cell suspension smoothly transitions to the filter screen 3 along the guide cone 11, thereby preventing larger cell agglomerates in the cell suspension from accumulating on the top of the filter screen 3.

[0037] By installing the guide cone 11 at the end of the filter mesh 3 , it is possible to prevent cell agglomerates from being accumulated on the top of the filter mesh 3 .

[0038] Embodiment 5:

[0039] like Figure 3-5 As shown, a second mounting frame 12 is installed at the end of the filter screen 3, and the second mounting frame 12 is fixedly connected to the support rod 10, so that the first mounting frame 9, the support rod 10 and the second mounting frame 12 are connected as a whole, and the second mounting frame 12 is also fixedly connected to the inner wall of the collection bag body 1, thereby improving the stability of the filter screen 3 during use.

[0040] By installing the second mounting frame 12 at the end of the filter screen 3, the stability of the filter screen 3 during use can be improved.

[0041] Working principle: When in use, the first use method is as follows: connect the feed pipe 2 to the bioreactor, connect the discharge pipe 4 to the centrifugal device, open the first control valve 5 and the second control valve 6, and discharge the cell suspension in the bioreactor from the feed pipe 2 into the collection bag body 1 for temporary storage, and the excess air in the collection bag body 1 is discharged out of the bag through the microfiltration filter element 8, and then the larger cell agglomerates in the cell suspension are intercepted by the filter net 3, and the rest will pass through the filter net 3 and be discharged into the centrifugal device through the discharge pipe 4 to carry out the next process, thereby realizing the continuous aseptic operation of biological cell preparation and reducing the risk of microbial contamination;

[0042] The second method of use is as follows: connect the feed pipe 2 to the bioreactor, open the first control valve 5, and close the second control valve 6, so that the cell suspension in the bioreactor can be discharged into the collection bag body 1 for storage, and then close the first control valve 5 to collect the cell suspension, then remove the feed pipe 2 from the bioreactor, move the device above the mouth of the centrifuge bottle, align the discharge pipe 4 with the bottle mouth, open the second control valve 6, and the cell suspension in the bag is filtered through the filter net 3 and discharged into the centrifuge bottle, and the external air is sterilized and filtered through the microfiltration filter element 8 and then enters the collection bag body 1, thereby realizing the collection, transfer and filtration of the cell suspension.

Claims

1. A cell filtration collection bag, characterized in that: include: A collecting bag body (1), wherein a feed pipe (2) is installed at the top of the collecting bag body (1), a discharge pipe (4) is installed at the bottom of the collecting bag body (1), a filter screen (3) is installed in the collecting bag body (1), an air pipe (7) is installed on the collecting bag body (1), a microfiltration filter element (8) is installed on the air pipe (7), and a first control valve (5) and a second control valve (6) are installed on the feed pipe (2) and the discharge pipe (4), respectively.

2. A cell filtration collection bag according to claim 1, characterized in that: The pore size of the filter screen (3) is 40-100 μm.

3. A cell filtration collection bag according to claim 1, characterized in that: The filter screen (3) is a flat plate.

4. A cell filtration collection bag according to claim 1, characterized in that: The filter screen (3) is in the shape of a cone barrel.

5. A cell filtration collection bag according to claim 4, characterized in that: A first mounting frame (9) is installed at the bottom of the filter screen (3), the first mounting frame (9) is connected to the collection bag body (1), a support rod (10) is installed on the first mounting frame (9), and the support rod (10) is connected to the top of the filter screen (3).

6. A cell filtration collection bag according to claim 5, characterized in that: A guide cone (11) is installed at the end of the filter screen (3).

7. A cell filtration collection bag according to claim 5, characterized in that: A second mounting frame (12) is mounted on the end of the filter screen (3), and the second mounting frame (12) is connected to the support rod (10).

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