Sample adding plate for purification

Abstract

The sample adding plate for purification comprises at least one group of sample adding areas, and each group of sample adding areas comprises three sample adding holes, namely a magnetic bead adsorption hole, at least one washing hole and at least one elution hole; magnetic beads are accommodated in the magnetic bead adsorption holes and are used for adsorbing biological molecules; the length of the magnetic bead adsorption hole is equal to the length of at least two standard holes; cleaning liquid is contained in the washing holes and is used for cleaning magnetic beads and biomolecules; the length of the washing hole is equal to the length of at least one standard hole; eluent is accommodated in the elution holes and is used for separating the magnetic beads and the biological molecules; and the length of the elution hole is at least the length of one standard hole. The magnetic sleeve rod is suitable for occasions requiring high-capacity purification, and is suitable for improving the mixing effect and the purification efficiency of the conventional magnetic sleeve rod.

Description

Technical Field

[0001] The utility model belongs to the technical field of molecular biological experimental equipment, and in particular relates to a sample adding plate for purification. Background Art

[0002] Existing magnetic bar extractors based on magnetic bead transfer generally use a kit made based on a standard 96-well deep-well plate to purify biological molecules such as nucleic acids / proteins. Since the capacity of each well of this kit is limited, the sample liquid that can be extracted and purified each time is limited.

[0003] Moreover, since the cross-sectional area of ​​a single well of a 96-well plate is very small, it is only suitable for the up and down movement of the magnetic rod sleeve, and the mixing effect on the reagents is limited.

[0004] Therefore, the prior art needs to be further improved and enhanced. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the purpose of the utility model is to provide a sample adding plate for a magnetic bead plasmid extractor, which can process more volumes of sample liquid to be adsorbed and purified at one time and is suitable for the horizontal movement of the existing magnetic rod sleeve.

[0006] The utility model discloses a sample adding plate for purification, comprising at least one group of sample adding areas, each group of sample adding areas comprising three sample adding holes, namely: a magnetic bead adsorption hole, at least one washing hole, and at least one elution hole;

[0007] The magnetic bead adsorption hole contains magnetic beads for adsorbing biomolecules; the length of the magnetic bead adsorption hole is at least the length of two standard holes;

[0008] The washing hole contains a washing liquid for washing the magnetic beads and biomolecules; the length of the washing hole is the length of at least one standard hole;

[0009] The elution hole contains an elution liquid for separating magnetic beads and biomolecules; the length of the elution hole is at least the length of a standard hole.

[0010] Preferably, the sample addition wells are deep wells arranged in sequence, and the cross-section of the deep wells is square.

[0011] Preferably, a smooth arc-shaped depression is provided in the center of the bottom of the sample addition well.

[0012] Preferably, the bottom of the sample addition hole is in a downwardly arched shape.

[0013] Preferably, more than one row of sample application areas is included, and each row includes at least two groups of sample application areas.

[0014] Preferably, the sample adding plate comprises 8 rows of sample adding areas, and each row comprises two groups of sample adding areas.

[0015] Preferably, it comprises a sealing film portion, and the sealing film portion is used to seal the sample adding area.

[0016] Preferably, the length of the washing hole is the length of two standard holes, and / or the length of the elution hole is the length of two standard holes.

[0017] Preferably, the length of the magnetic bead adsorption hole is twice the length of the washing hole and / or twice the length of the elution hole.

[0018] Preferably, the sample addition area further comprises an extraction hole, wherein the extraction hole is used to extract the biomolecules in the sample liquid, and at least a lysis solution is provided in the extraction hole, which is used to lyse and release the biomolecules in the sample liquid.

[0019] Preferably, the extraction hole includes at least one horizontally arranged partition membrane, which divides the extraction hole into at least two layers in sequence, namely a lysis layer and a neutralization layer, the lysis layer contains a lysis liquid, and the neutralization layer contains a neutralization liquid. The partition membrane is made of an inert material that is easy to break. Preferably, the thickness of the partition membrane is suitable for being adjusted to be pierced through at one time; and / or the partition membrane is cast and solidified by heating a material whose solidification temperature is higher than the ambient temperature to a molten state. Preferably, the material of the partition membrane is selected from paraffin.

[0020] The sample adding plate for purification of the utility model is provided with a magnetic bead adsorption hole, a cleaning hole and an elution hole. Before purification, a reagent liquid to be adsorbed and purified is added to the magnetic bead adsorption hole, and a magnetic rod sleeve provided with a magnetic rod is moved horizontally and vertically, and after mixing, it is moved downward to the magnetic bead adsorption hole. The magnetic rod adsorbs magnetic beads by magnetism, and the magnetic beads adsorb nucleic acid / protein from the solution. After being lifted up with the magnetic rod, the magnetic rod is inserted into the cleaning hole to clean the nucleic acid / protein. After cleaning, the magnetic rod is inserted into the elution hole, and the magnetic beads and the adsorbed biological molecules, such as nucleic acid or protein, are separated by an elution liquid. Since the volume of the magnetic bead adsorption hole is increased, the utility model is suitable for occasions requiring large-capacity purification, and is suitable for the existing magnetic rod sleeve to move horizontally, thereby increasing the mixing degree, so that the reagent liquid can be purified more fully, and the purification efficiency is improved.

[0021] The concept, specific structure and technical effects of the present invention will be further described below in conjunction with the accompanying drawings to fully understand the purpose, characteristics and effects of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural diagram of a 96-well deep-well plate in the prior art;

[0023] Figure 2 It is a structural diagram of the sample plate of the utility model;

[0024] Figure 3 Another perspective structural diagram of the sample plate of the utility model;

[0025] In the figure,

[0026] 100, 96-well deep well plate, 110, standard well;

[0027] 200, sample adding plate, 210, magnetic bead adsorption well, 220, washing well, 230, elution well; DETAILED DESCRIPTION

[0028] The utility model provides a sample adding plate for a magnetic bead method plasmid extraction instrument. In order to make the purpose, technical solution and effect of the utility model clearer and more specific, the utility model is further described in detail with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0029] like Figure 1 As shown, it is a schematic diagram of the structure of a 96-well deep-well plate 100 commonly used in the art. In the present utility model, for the convenience of description, we define a single hole in the 96-well deep-well plate 100 as a deep hole, and also define a deep hole as a standard hole 110. Biomolecules can be nucleic acids, proteins, plasmids, etc., including but not limited to the above examples. All biomolecules that can be purified by magnetic beads are within the protection scope of the present application.

[0030] The utility model provides a sample adding plate 200 for purification, comprising at least one group of sample adding areas, each group of sample adding areas comprising three sample adding holes, namely: a magnetic bead adsorption hole 210, at least one washing hole 220, and at least one elution hole 230;

[0031] The magnetic bead adsorption hole 210 contains magnetic beads for adsorbing biomolecules; the length of the magnetic bead adsorption hole 210 is at least the length of two standard holes 110, for example, it can be the length of two, three, four or five standard holes. By extending the length of the hole to at least two existing standard holes, it is suitable for the existing magnetic rod sleeve (including the case of inserting or not inserting the magnetic rod) to move horizontally in the hole, thereby increasing the mixing intensity and facilitating the improvement of purification efficiency;

[0032] The washing hole 220 contains a washing liquid for washing the magnetic beads and biomolecules; the length of the washing hole 220 is the length of at least one standard hole 110;

[0033] The elution hole 230 contains an elution liquid for separating magnetic beads and biomolecules; the length of the elution hole 230 is at least the length of a standard hole 110 .

[0034] As another embodiment of the sample loading plate for purification, the length of the washing hole 220 is the length of two standard holes 110 , and / or the length of the elution hole 220 is the length of two standard holes 110 .

[0035] Specifically, Figure 2 and Figure 3 As shown, the sample adding plate 200 includes more than one row of the sample adding areas, each row includes at least two groups of sample adding areas, and the sample adding plate includes 8 rows of sample adding areas, each row includes two groups of sample adding areas. Taking the length of the magnetic bead adsorption hole 210 as the length of two standard holes 110 as an example, it can also be understood that Figure 1 The adjacent two standard holes 110 in the 96-well deep well plate 100 are opened; the length of the cleaning hole 220 and the elution hole 230 is the length of the two standard holes 110. Similarly, it can be understood that Figure 1 Two adjacent standard wells 110 in the 96-well deep well plate 100 are opened.

[0036] As a third embodiment of the sample loading plate for purification, the length of the magnetic bead adsorption hole 210 is twice the length of the washing hole 220 and / or twice the length of the elution hole 230 .

[0037] Specifically, Figure 2 As shown, the sample addition wells are deep holes arranged in sequence, and the cross-section of the deep holes is square. The regular shape facilitates the maximum utilization of the space of the sample addition plate, and the cross-sectional area of ​​the deep holes is not limited to this. This application provides an embodiment. In actual use, it does not affect the extraction of biological molecules such as nucleic acids / proteins, and the shape design that maximizes the utilization of space is within the scope of protection of this application.

[0038] Furthermore, a smooth arc-shaped downward depression is provided in the center of the bottom of the sample loading hole, which is not shown in the figure, to facilitate the precipitation of the sample liquid, which gathers at the bottom of the sample loading hole and is beneficial to the adsorption and purification of the magnetic beads.

[0039] Furthermore, the bottom of the sample loading hole is in a downwardly arched shape, which is not shown in the figure. When the magnetic rod and the magnetic rod sleeve are inserted into the sample loading hole, the end of the magnetic rod sleeve is accommodated to play a positioning role.

[0040] In order to facilitate the movement and transportation of the sample loading plate 200 and standardized extraction, the sample loading plate 200 further includes a sealing film layer, which is not shown in the figure. The sealing film layer is used to seal the sample loading area to prevent the reagent liquid from leaking out.

[0041] Furthermore, the sample addition area further comprises an extraction hole, and the extraction hole is used to extract the biological molecules in the sample liquid. At least a lysis solution is arranged in the extraction hole, and is used to lyse and release the biological molecules in the sample liquid.

[0042] Furthermore, the extraction hole includes at least one horizontally arranged partition membrane, and the partition membrane sequentially divides the extraction hole into at least two layers, namely a lysis layer and a neutralization layer, wherein the lysis layer contains a lysis solution, and the neutralization layer contains a neutralization solution. The "horizontally arranged" herein refers to the partition membrane being placed horizontally or vertically, that is, the partition membrane is placed as non-inclined as possible. The "sequentially" herein refers to being arranged in the described order, so as to meet the order required for process completion.

[0043] Furthermore, the thickness of the separation membrane is suitable for being adjusted to be pierced once, and the separation membrane is made of a soft inert material or film that is easy to break.

[0044] Furthermore, the separation membrane is formed by casting and curing after heating a material having a curing temperature higher than the ambient temperature to a molten state, and the material of the separation membrane is paraffin.

[0045] In the present application, the "membrane" in the separator membrane has no special meaning, that is, it is not limited to the thickness or material of the commonly described membrane, but any membrane that can be easily broken during use and can perform horizontal separation.

[0046] The sample adding plate for purification of the utility model is provided with a magnetic bead adsorption hole, a cleaning hole and an elution hole. Before purification, a reagent liquid to be adsorbed and purified is added to the magnetic bead adsorption hole, and a magnetic rod sleeve with a magnetic rod is moved horizontally and up and down, and after mixing, it is moved downward to the magnetic bead adsorption hole. The magnetic rod adsorbs magnetic beads by magnetism, and the magnetic beads adsorb nucleic acid / protein from the solution. After being lifted up with the magnetic rod, it is inserted into the cleaning hole to clean the nucleic acid / protein. After cleaning, it is inserted into the elution hole, and the magnetic beads and the adsorbed nucleic acid / protein are separated by the elution liquid. Since the volume of the magnetic bead adsorption hole is increased, it is suitable for occasions requiring large-capacity purification, and is suitable for the existing magnetic rod sleeve with a magnetic rod to move horizontally, thereby improving the mixing effect, so that the reagent liquid can be purified more fully, and the purification efficiency is improved.

[0047] The preferred specific embodiments of the utility model are described in detail above. It should be understood that ordinary technicians in this field can make many modifications and changes based on the concept of the utility model without creative work. Therefore, all technical solutions that can be obtained by technicians in this technical field based on the concept of the utility model through logical analysis, reasoning or limited experiments on the basis of the existing technology should be within the scope of protection determined by the claims.

Claims

1. A sample adding plate for purification, characterized in that: It comprises at least one group of sample loading areas, each group of sample loading areas comprises three sample loading holes, namely: a magnetic bead adsorption hole (210), at least one washing hole (220), and at least one elution hole (230); The magnetic bead adsorption hole (210) contains magnetic beads for adsorbing biomolecules; the length of the magnetic bead adsorption hole (210) is at least the length of two standard holes (110); The washing hole (220) contains a washing liquid for washing the magnetic beads and biomolecules; the length of the washing hole (220) is the length of at least one standard hole (110); The elution hole (230) contains an elution liquid for separating magnetic beads and biomolecules; the length of the elution hole (230) is at least the length of a standard hole (110).

2. A sample adding plate for purification according to claim 1, characterized in that: The sample addition holes are deep holes arranged in sequence, and the cross section of the deep holes is square.

3. A sample adding plate for purification according to claim 1, characterized in that: A smooth arc-shaped depression is arranged in the center of the bottom of the sample adding hole.

4. A sample adding plate for purification according to claim 1, characterized in that: The bottom of the sample adding hole is in a downward arch shape.

5. A sample adding plate for purification according to claim 1, characterized in that: The sample application area comprises more than one row, and each row comprises at least two groups of sample application areas.

6. A sample adding plate for purification according to claim 5, characterized in that: The sample adding plate comprises 8 rows of sample adding areas, and each row comprises two groups of sample adding areas.

7. A sample adding plate for purification according to claim 1, characterized in that: It comprises a sealing film portion, and the sealing film portion is used to seal the sample adding area.

8. The purification sample adding plate according to any one of claims 1 to 7, characterized in that: The length of the washing hole (220) is the length of two standard holes (110), and / or the length of the elution hole (230) is the length of two standard holes (110).

9. The purification sample adding plate according to any one of claims 1 to 7, characterized in that: The length of the magnetic bead adsorption hole (210) is twice the length of the washing hole (220) and / or twice the length of the elution hole (230).

10. The sample adding plate for purification according to any one of claims 1 to 9, characterized in that: The sample adding area further comprises an extraction hole, wherein the extraction hole is used to extract the biomolecules in the sample liquid. Lysis liquid is arranged in the extraction hole, and is used to lyse and release the biomolecules in the sample liquid.

11. The purification sample adding plate according to claim 10, characterized in that: The extraction hole includes at least one horizontally arranged separation membrane, which divides the extraction hole into at least two layers in sequence, namely a lysis layer and a neutralization layer. The lysis layer contains a lysis solution, and the neutralization layer contains a neutralization solution. The separation membrane is made of an inert material that is easy to break.

12. The sample adding plate for purification according to claim 11, characterized in that: The thickness of the separation film is suitable for being adjusted to be penetrated once; and / or The separation membrane is formed by casting and curing a material whose curing temperature is higher than the ambient temperature after heating it to a molten state.

13. The sample adding plate for purification according to claim 12, characterized in that: The material of the separation membrane is selected from paraffin.

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