Culture water bath box capable of stably taking NK cell culture dishes

Abstract

The utility model discloses a culture water bath box capable of stably taking an NK cell culture dish, and belongs to the technical field of NK cell culture equipment. Comprising a constant-temperature water bath box, a lifting mechanism, an NK cell culture dish, a clamping mechanism and a bearing plate, the constant-temperature water bath box is used for carrying out water bath on NK cell culture, the lifting mechanism is arranged on one side and in the constant-temperature water bath box, and the lifting mechanism is used for lifting the bearing plate. The clamping mechanism is arranged, so that the NK cell culture dish can be directly clamped and fixed, the NK cell culture dish cannot shake violently when being taken, the NK cell culture dish can be taken without large force, internal devices are prevented from being damaged, and the effect of protecting the NK cells in the NK cell culture dish is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of NK cell culture equipment, and more specifically, to a culture water bath capable of stably taking NK cell culture dishes. Background Art

[0002] NK cells are natural killer cells (NK), which are the first line of defense of the human immune system. They are closely related to the body's anti-tumor and immune regulation functions. They can widely identify, quickly dissolve, kill, and destroy cancer cells. NK cells have a strong killing effect on tumor cells from different sources and have good application and development prospects in the immune cell therapy of tumors. Constant temperature water bath is an essential tool for NK cell culture.

[0003] The prior art document with publication number CN216614699U provides a constant temperature water bath device for cell culture, which can clamp and fix a culture dish by means of a spring, a connecting column, a clamping plate and an anti-slip layer.

[0004] In the related art, the culture dish can be clamped and fixed by providing a spring, a connecting column, a clamping plate and an anti-slip layer.

[0005] Although the above-mentioned prior art solution can achieve the effect of clamping the culture dish by setting up the spring, connecting column, splint and anti-slip layer, it still has the following defects: when removing the culture dish, it is necessary to take it off with force, which is easy to damage other devices inside the water bath, and the shaking caused by it may cause the cells in the culture dish to rupture.

[0006] In view of this, we propose a culture water bath that can stably pick up NK cell culture dishes. Utility Model Content

[0007] 1. Technical problems to be solved

[0008] The purpose of the present application is to provide a culture water bath that can stably pick up NK cell culture dishes, thereby solving the technical problem that the culture dish needs to be removed with force, which can easily damage other devices inside the water bath and cause shaking that may cause cells in the culture dish to rupture. The present application achieves the technical effect of not requiring the use of too much force, preventing the NK cell culture dish from shaking violently, protecting the NK cells inside it, and not causing damage to the internal devices.

[0009] 2. Technical solution

[0010] The embodiment of the present application provides a culture water bath that can stably take NK cell culture dishes, including a constant temperature water bath, a lifting mechanism, a NK cell culture dish, a clamping mechanism and a carrying plate;

[0011] A constant temperature water bath, wherein the constant temperature water bath is used for water bathing of NK cell culture;

[0012] A lifting mechanism, which is arranged on one side and inside of the constant temperature water bath and is used for lifting and lowering the carrying plate;

[0013] NK cell culture dish, the NK cell culture dish is arranged inside the constant temperature water bath, and a plurality of the NK cell culture dishes are arranged, and the NK cell culture dish is used to hold NK cells;

[0014] A clamping mechanism, wherein the clamping mechanism is symmetrically arranged at the top and bottom of the carrying plate, and the clamping mechanism is used to automatically clamp and fix the NK cell culture dish;

[0015] A carrying plate, wherein the carrying plate is arranged inside a constant temperature water bath, a plurality of the carrying plates are arranged, and the carrying plates are fixedly connected to each other, and the carrying plate is used for placing NK cell culture dishes.

[0016] By adopting the above technical solution and setting the lifting mechanism, the NK cell culture dish can be smoothly lowered into the water for a water bath, and can be driven to rise, so that it is easy to take it out and use it conveniently. The setting of the clamping mechanism can directly clamp and fix the NK cell culture dish, and the NK cell culture dish will not be shaken violently when it is taken out, and it can be taken out without using great force, thereby preventing damage to the internal device, thereby achieving a protective effect on the NK cells inside it.

[0017] As an optional solution of the technical solution of the present application document, the lifting mechanism also includes a motor, a driving pulley is fixedly provided at the driving end of the motor, and the driving pulley is respectively connected to a driven pulley A and a driven pulley B through a synchronous belt, one end of the driven pulley B is fixedly provided with a reverse gear, and the outer side of the reverse gear is meshed with a linkage gear, one end of the driven pulley A and the linkage gear are fixedly provided with a linkage rod, and the outer side of the linkage rod is fixedly provided with two lifting gears, and the outer side of the lifting gear is meshed with a lifting plate, and the lifting plates are fixedly provided on two sides of the load-bearing plate away from each other.

[0018] By adopting the above technical solution, the setting of the lifting mechanism can smoothly lower the NK cell culture dish into the water for water bathing, and can also drive the NK cell culture dish to rise, so that it is easy to take it out and use it conveniently.

[0019] As an optional solution to the technical solution of the present application document, the motor is fixedly arranged on one side of the constant temperature water bath, the driving pulley and the reverse gear are both rotatably arranged on one side of the constant temperature water bath, the linkage rod is rotatably arranged on one side of the interior of the constant temperature water bath, and the linkage rod passes through and is rotatably arranged on the side of the constant temperature water bath where the motor is arranged.

[0020] By adopting the above technical solution, the lifting mechanism can better achieve the lifting effect.

[0021] As an optional solution to the technical solution of the present application document, the clamping mechanism also includes a clamping jaw, a vertical block is rotatably arranged on one side of the clamping jaw, a lever is fixedly arranged on the other side of the clamping jaw, a spring and a telescopic rod are arranged at the bottom end of the clamping jaw, a convex sliding block is fixedly arranged at one end of the spring and the telescopic rod, and a fixed seat frame is fixedly arranged at the other end of the spring and the telescopic rod.

[0022] By adopting the above technical solution, the setting of the clamping mechanism can directly clamp and fix the NK cell culture dish, and the NK cell culture dish will not be shaken violently when it is taken, and it can be taken without using great force to prevent damage to the internal device, thereby achieving a protective effect on the NK cells inside.

[0023] As an optional solution of the technical solution of the present application document, the upright block is fixedly arranged at the top end of the supporting plate, the fixed seat frame is fixedly arranged at the bottom end of the supporting plate, and the clamp is arranged in a "C" shape.

[0024] By adopting the above technical solution, the clamping mechanism can better clamp and fix the NK cell culture dish.

[0025] As an optional solution of the technical solution of the present application document, a guide rail is fixedly provided on the outer side of the NK cell culture dish, a bottom column is fixedly provided on the bottom end of the NK cell culture dish, slots are symmetrically provided on the outer side of the bottom column, and a groove is provided on the bottom end of the bottom column.

[0026] By adopting the above technical solution, it is convenient to position, place, clamp and fix the NK cell culture dish.

[0027] As an optional solution of the technical solution of the present application document, a guide plate is fixedly provided on one side of the supporting plate, through grooves are symmetrically opened at the top of the supporting plate, a guide column is fixedly provided in the middle of the top of the supporting plate, and the guide plate is slidably provided on the outside of the guide rail.

[0028] By adopting the above technical solution, the setting of the carrier plate facilitates the positioning and placement of the NK cell culture dish.

[0029] 3. Beneficial effects

[0030] One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

[0031] 1. The present application can smoothly lower the NK cell culture dish into the water for water bathing by setting the lifting mechanism, and can also drive the NK cell culture dish to rise, so that it is easy to take it and use;

[0032] 2. The present application sets a clamping mechanism, which can directly clamp and fix the NK cell culture dish, and the NK cell culture dish will not be shaken violently when it is taken, and it can be taken without using great force to prevent damage to the internal device, thereby achieving a protective effect on the NK cells inside. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a schematic diagram of the overall structure of a culture water bath capable of stably picking up NK cell culture dishes disclosed in a preferred embodiment of the present application;

[0034] Figure 2 This is a schematic diagram of the overall structure of the interior of a constant temperature water bath in a water bath for NK cell culture disclosed in a preferred embodiment of the present application;

[0035] Figure 3 This is a schematic diagram of the combined structure of a lifting mechanism and a NK cell culture dish in a culture water bath that can stably take out a NK cell culture dish disclosed in a preferred embodiment of the present application;

[0036] Figure 4 This is a schematic diagram of the disassembly structure of the NK cell culture dish, the clamping mechanism and the carrying plate in the culture water bath that can stably pick up the NK cell culture dish disclosed in a preferred embodiment of the present application;

[0037] Explanation of the numbers in the figure: 1. Constant temperature water bath; 2. Lifting mechanism; 201. Motor; 202. Driving pulley; 203. Synchronous belt; 204. Driven pulley A; 205. Driven pulley B; 206. Reverse gear; 207. Linkage gear; 208. Linkage rod; 209. Lifting plate; 210. Lifting gear; 3. NK cell culture dish; 301. Guide rail; 302. Bottom column; 4. Clamping mechanism; 401. Clamping claw; 402. Vertical block; 403. Spring; 404. Telescopic rod; 405. Fixed seat; 406. Push rod; 5. Loading plate; 501. Guide plate; 502. Through groove; 503. Guide column. DETAILED DESCRIPTION

[0038] The present application is further described in detail below in conjunction with the accompanying drawings.

[0039] Reference Figure 1 and Figure 2, the embodiment of the present application discloses a culture water bath that can stably pick up NK cell culture dishes, including a constant temperature water bath 1, a lifting mechanism 2, a NK cell culture dish 3, a clamping mechanism 4 and a carrying plate 5;

[0040] Constant temperature water bath 1, constant temperature water bath 1 is used for water bathing of NK cell culture;

[0041] A lifting mechanism 2, which is disposed on one side and inside of the constant temperature water bath 1, and is used for lifting and lowering the carrying plate 5;

[0042] NK cell culture dish 3, the NK cell culture dish 3 is arranged inside the constant temperature water bath 1, a plurality of NK cell culture dishes 3 are provided, and the NK cell culture dishes 3 are used to hold NK cells;

[0043] A clamping mechanism 4, which is symmetrically arranged at the top and bottom of the carrying plate 5, and is used to automatically clamp and fix the NK cell culture dish 3;

[0044] The carrying plate 5 is arranged inside the constant temperature water bath 1 . A plurality of carrying plates 5 are provided. The carrying plates 5 are fixedly connected to each other. The carrying plates 5 are used to place the NK cell culture dish 3 .

[0045] By setting the lifting mechanism 2, the NK cell culture dish 3 can be smoothly lowered into the water for a water bath, and can be driven to rise, so that it is easy to take it out and use it conveniently. The setting of the clamping mechanism 4 can directly clamp and fix the NK cell culture dish 3, and the NK cell culture dish 3 will not be shaken violently when it is taken out, and it can be taken out without using great force, thereby preventing damage to the internal device, thereby achieving a protective effect on the NK cells inside it.

[0046] Reference Figure 2 and Figure 3The lifting mechanism 2 also includes a motor 201, a driving pulley 202 is fixedly provided at the driving end of the motor 201, and the driving pulley 202 is respectively connected to a driven pulley A204 and a driven pulley B205 through a synchronous belt 203, and a reverse gear 206 is fixedly provided at one end of the driven pulley B205, and a linkage gear 207 is meshed on the outer side of the reverse gear 206, and a linkage rod 208 is fixedly provided at one end of the driven pulley A204 and the linkage gear 207, and the outer side of the linkage rod 208 Two lifting gears 210 are fixedly set on both sides, and lifting plates 209 are meshed on the outer sides of the lifting gears 210. The lifting plates 209 are fixedly set on both sides of the carrying plate 5 away from each other. The motor 201 is fixedly set on one side of the constant temperature water bath 1, and the driving pulley 202 and the reverse gear 206 are both rotatably set on one side of the constant temperature water bath 1. The linkage rod 208 is rotatably set on one side of the interior of the constant temperature water bath 1, and the linkage rod 208 passes through and is rotatably set on the side of the constant temperature water bath 1 where the motor 201 is set.

[0047] By starting the motor 201 in the lifting mechanism 2, the active pulley 202 can drive the driven pulley A204 and the driven pulley B205 to rotate through the synchronous belt 203. The rotation of the driven pulley B205 drives the reverse gear 206 to rotate, and the reverse gear 206 drives the linkage gear 207 to rotate, so that the rotation direction of the linkage gear 207 is opposite to that of the driven pulley A204. The driven pulley A204 and the linkage gear 207 simultaneously drive the linkage rod 208 and the lifting gear 210 to rotate, so that the lifting plate 209 descends, and the NK cell culture dish 3 is lowered into the water for a water bath, which is convenient for the NK cells to be water bathed. The lifting is stable and simple and convenient to use.

[0048] Reference Figure 2 and Figure 4 The clamping mechanism 4 also includes a clamping jaw 401, a vertical block 402 is rotatably provided on one side of the clamping jaw 401, a lever 406 is fixedly provided on the other side of the clamping jaw 401, a spring 403 and a telescopic rod 404 are provided at the bottom end of the clamping jaw 401, a convex sliding block is fixedly provided at one end of the spring 403 and the telescopic rod 404, a fixed seat frame 405 is fixedly provided at the other end of the spring 403 and the telescopic rod 404, the vertical block 402 is fixedly provided at the top end of the supporting plate 5, the fixed seat frame 405 is fixedly provided at the bottom end of the supporting plate 5, and the clamping jaw 401 is provided in a "C" shape.

[0049] The clamping jaw 401 in the clamping mechanism 4 is in an inclined state when not in use, and the spring 403 and the telescopic rod 404 are at their original lengths. When the NK cell culture dish 3 is pressed, the guide column 503 will enter the inner side of the groove at the bottom end of the bottom column 302, and at the same time, the bottom column 302 will press the protruding corner of one side of the bottom end of the clamping jaw 401. The protruding part of the top end of the clamping jaw 401 is smaller than the length of the protruding part of the bottom end of the clamping jaw 401, so that the bottom column 302 can be pressed, and then the clamping jaw 401 will slowly stand upright, and the clamping During the erection process of the claw 401, the protruding part of its top is inserted into the groove provided on the outside of the bottom column 302, and the clamping claw 401 will slide on the outside of the convex sliding block and compress the spring 403 and the telescopic rod 404. Since the spring 403 provides an axial lifting force, the clamping claw 401 locks the bottom column 302 at this time to complete the fixation of the NK cell culture dish 3. When the NK cell culture dish 3 needs to be taken down, the two levers 406 are pushed by the fingers at the same time to tilt the clamping claw 401 to the outside.

[0050] Reference Figure 2 and Figure 4 A guide rail 301 is fixedly arranged on the outer side of the NK cell culture dish 3, a bottom column 302 is fixedly arranged on the bottom end of the NK cell culture dish 3, a card slot is symmetrically provided on the outer side of the bottom column 302, a groove is provided on the bottom end of the bottom column 302, a guide plate 501 is fixedly arranged on one side of the supporting plate 5, a through groove 502 is symmetrically provided on the top of the supporting plate 5, a guide column 503 is fixedly arranged in the middle of the top end of the supporting plate 5, and the guide plate 501 is slidably arranged on the outer side of the guide rail 301.

[0051] The guide rail 301 cooperates with the guide plate 501 to position the NK cell culture dish 3 , and the guide plate 501 cooperates with the groove at the bottom of the bottom column 302 to achieve a positioning effect.

[0052] Working principle: put the NK cell culture dish 3 on the top of the carrier plate 5, and the guide rail 301 faces one side of the guide plate 501 to achieve the positioning effect, and then gently press down the NK cell culture dish 3, while the clamping jaw 401 in the clamping mechanism 4 is in an inclined state when not in use, and the spring 403 and the telescopic rod 404 are at their original lengths. When the NK cell culture dish 3 is pressed, the guide column 503 will enter the inner side of the groove at the bottom end of the bottom column 302, and at the same time, the bottom column 302 will press the protruding corner of one side of the bottom end of the clamping jaw 401, and the protruding part of the top end of the clamping jaw 401 is smaller than the length of the protruding part of the bottom end of the clamping jaw 401, so that the bottom column 302 can press, and then the clamping jaw 401 will slowly stand upright, When the clamp 401 is erected, the protruding part of the top thereof is inserted into the slot provided on the outside of the bottom column 302, and the clamp 401 slides on the outside of the convex slider and compresses the spring 403 and the telescopic rod 404. Since the spring 403 provides an axial lifting force, the clamp 401 locks the bottom column 302 at this time to complete the fixation of the NK cell culture dish 3. When the NK cell culture dish 3 needs to be taken down, the two levers 406 are simultaneously moved by fingers to tilt the clamp 401 outward. In this way, when the NK cell culture dish 3 is taken down, it will not be shaken violently, and it can be taken down without using a large force, thereby preventing damage to the internal device, thereby achieving a protective effect on the NK cells inside.

[0053] Then the motor 201 in the lifting mechanism 2 can be started, and the active pulley 202 can drive the driven pulley A204 and the driven pulley B205 to rotate through the synchronous belt 203. The rotation of the driven pulley B205 drives the reverse gear 206 to rotate, and the reverse gear 206 drives the linkage gear 207 to rotate, so that the rotation direction of the linkage gear 207 is opposite to that of the driven pulley A204. The driven pulley A204 and the linkage gear 207 simultaneously drive the linkage rod 208 and the lifting gear 210 to rotate, so that the lifting plate 209 descends, and the NK cell culture dish 3 is lowered into the water for a water bath, which is convenient for the NK cells to be water bathed. The lifting is stable and simple and convenient to use.

Claims

1. A culture water bath capable of stably taking NK cell culture dishes, characterized in that: It comprises: a constant temperature water bath (1), a lifting mechanism (2), a NK cell culture dish (3), a clamping mechanism (4) and a carrying plate (5); A constant temperature water bath (1), wherein the constant temperature water bath (1) is used for water bathing NK cell culture; A lifting mechanism (2), the lifting mechanism (2) being arranged on one side and inside the constant temperature water bath (1), the lifting mechanism (2) being used for lifting and lowering the carrying plate (5); NK cell culture dish (3), the NK cell culture dish (3) is arranged inside the constant temperature water bath (1), a plurality of the NK cell culture dishes (3) are arranged, and the NK cell culture dishes (3) are used to hold NK cells; A clamping mechanism (4), wherein the clamping mechanism (4) is symmetrically arranged at the top and bottom of the carrier plate (5), and the clamping mechanism (4) is used to automatically clamp and fix the NK cell culture dish (3); A carrying plate (5), wherein the carrying plate (5) is arranged inside the constant temperature water bath (1), a plurality of the carrying plates (5) are provided, and the carrying plates (5) are all fixedly connected to each other. The carrying plates (5) are used to place the NK cell culture dish (3).

2. The culture water bath capable of stably taking NK cell culture dishes according to claim 1, characterized in that: The lifting mechanism (2) further comprises a motor (201), a driving pulley (202) being fixedly provided at the driving end of the motor (201), the driving pulley (202) being respectively connected to a driven pulley A (204) and a driven pulley B (205) through a synchronous belt (203), a reverse gear (206) being fixedly provided at one end of the driven pulley B (205), a linkage gear (207) being meshedly provided at the outer side of the reverse gear (206), a linkage rod (208) being fixedly provided at one end of each of the driven pulley A (204) and the linkage gear (207), two lifting gears (210) being fixedly provided at the outer side of each of the linkage rods (208), a lifting plate (209) being meshedly provided at the outer side of each of the lifting gears (210), and the lifting plate (209) being fixedly provided at two sides of the carrying plate (5) which are away from each other.

3. The culture water bath capable of stably taking NK cell culture dishes according to claim 2, characterized in that: The motor (201) is fixedly arranged on one side of the constant temperature water bath (1), the driving pulley (202) and the reverse gear (206) are both rotatably arranged on one side of the constant temperature water bath (1), the linkage rod (208) is rotatably arranged on one side of the interior of the constant temperature water bath (1), and the linkage rod (208) penetrates and is rotatably arranged on one side of the constant temperature water bath (1) where the motor (201) is arranged.

4. The culture water bath capable of stably taking NK cell culture dishes according to claim 1, characterized in that: The clamping mechanism (4) further comprises a clamping jaw (401), one side of which is rotatably provided with a vertical block (402), the other side of which is fixedly provided with a lever (406), the bottom end of which is provided with a spring (403) and a telescopic rod (404), one end of which is fixedly provided with a convex sliding block, and the other end of which is fixedly provided with a fixed seat frame (405).

5. The culture water bath capable of stably taking NK cell culture dishes according to claim 4, characterized in that: The upright block (402) is fixedly arranged at the top end of the carrying plate (5), the fixed seat frame (405) is fixedly arranged at the bottom end of the carrying plate (5), and the clamping claw (401) is arranged in a "C" shape.

6. The culture water bath capable of stably taking NK cell culture dishes according to claim 1, characterized in that: A guide rail (301) is fixedly arranged on the outer side of the NK cell culture dish (3), a bottom column (302) is fixedly arranged on the bottom end of the NK cell culture dish (3), a card slot is symmetrically provided on the outer side of the bottom column (302), and a groove is provided on the bottom end of the bottom column (302).

7. The culture water bath capable of stably taking NK cell culture dishes according to claim 1, characterized in that: A guide plate (501) is fixedly arranged on one side of the bearing plate (5), a through groove (502) is symmetrically provided at the top of the bearing plate (5), a guide column (503) is fixedly arranged in the middle of the top of the bearing plate (5), and the guide plate (501) is slidably arranged on the outer side of the guide rail (301).

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