Petri dish punching device

Abstract

The utility model relates to a culture dish punching device which comprises a mounting part and a punching part, and the mounting part is provided with a mounting groove; the punching part is detachably connected with the mounting part, the punching part comprises a fixing part and a punching part which are connected with each other, the fixing part comprises a first end and a second end which are oppositely arranged, the first end is contained in the mounting groove, and the second end is connected with the punching part; and the punching pieces with different sizes can be replaced relative to the mounting piece. By replacing punching pieces with different sizes on the mounting piece, micropores with different sizes can be prepared in the culture dish, so that the problem that micropores with different sizes need to be prepared for culturing embryos of different species in different scientific research experiments is effectively solved, namely, the punching device can meet the requirement of adapting to culture of different species; in addition, through the detachable arrangement of the mounting part and the punching part, when the micropores with different sizes are prepared, only the punching part needs to be replaced, the mounting part and the punching part do not need to be wholly replaced, and the production cost is effectively reduced.

Description

Technical Field

[0001] The present application relates to the field of embryo culture technology, and in particular to a culture dish punching device. Background Art

[0002] In the culture of animal embryos, the method of culturing embryos with micro-holes (hereinafter referred to as micro-holes) has become the choice of many scientific researchers. By placing a single embryo in a micro-hole for culture, the good development of a single embryo can be achieved, which can improve the blastocyst development rate and production efficiency of the embryo. In the related art, the production of micro-holes is achieved by punching holes at the bottom of a cell culture dish with a punching device. The function of the punching device is to form a small depression (micro-hole) with smooth sides and no gaps at the bottom of the tissue culture dish by pressing, so that the embryo and cells can gather in the micro-hole.

[0003] Based on different scientific research experiments and taking into account the differences in embryo size of different species, different embryos have different requirements for microwell preparation when cultured in microwells. For example, the diameter of a mouse embryo is about 80 μm, and the diameter of a monkey embryo is about 120 μm. However, the microwell manufacturing specifications in the existing technology are single, which cannot meet the needs of embryo culture of different species and has low adaptability.

[0004] Therefore, how to meet the needs of embryo culture of different species and improve the compatibility of embryos of different species with microwells is one of the issues that technicians in this field need to consider. Utility Model Content

[0005] Based on this, it is necessary to provide a culture dish punching device to address the problem that the micropores have a single specification, cannot meet the needs of embryo culture of different species, and have low adaptability.

[0006] The first aspect of the present application provides a culture dish punching device, which includes a mounting piece and a punching piece, the mounting piece is provided with a mounting groove; the punching piece is detachably connected to the mounting piece, the punching piece includes a fixing portion and a punching portion that are connected to each other, the fixing portion includes a first end and a second end that are relatively arranged, the first end is accommodated in the mounting groove, and the second end is connected to the punching portion, and the punching portion is used to form a hole on the culture dish; wherein punching pieces of different sizes can be replaced relative to the mounting piece.

[0007] In one embodiment, the perforated portion is spherical, and the diameter of the perforated portion is in the range of 0.3 mm to 1 mm.

[0008] In one of the embodiments, a first mark is provided on the outer peripheral surface of the fixing portion, and the first mark is used to indicate the punching depth of the punching portion.

[0009] In one embodiment, a second mark is provided on the outer peripheral surface of the first end, and the second mark is used to indicate the diameter size of the punching portion.

[0010] In one embodiment, the second end is provided with a receiving groove, and the receiving groove is used to install the punching part.

[0011] In one embodiment, the culture dish punching device further includes a protective member, which is detachably connected to the mounting member and is used to seal the punching member.

[0012] In one embodiment, the protective member includes an opening and a protective space that are interconnected, the opening is used for allowing at least part of the mounting member and the punching member to enter and exit the protective space, and the inner wall of the protective member at the opening can fit with the outer wall of the mounting member.

[0013] In one of the embodiments, the culture dish punching device further comprises an operating member, which is disposed at an end of the mounting member away from the punching member, and the operating member can drive the mounting member to move under the action of an external force.

[0014] In one embodiment, the operating member includes a pressing portion, a connecting portion and a supporting portion, the connecting portion is connected between the pressing portion and the supporting portion, the pressing portion is connected to the mounting member, the pressing portion is used to abut against a finger, and the supporting portion is used to abut against a palm.

[0015] In one embodiment, the operating member further includes an anti-slip portion, and the anti-slip portion is disposed on the pressing portion and / or the supporting portion.

[0016] In the above-mentioned culture dish punching device, by replacing punching pieces of different sizes on the mounting piece, microholes of different sizes can be prepared on the culture dish, which effectively solves the problem of preparing microholes of different sizes for culturing embryos of different species in different scientific research experiments, that is, the punching device can meet the needs of adapting to the cultivation of different species; in addition, through the detachable setting of the mounting piece and the punching piece, when preparing microholes of different sizes, it is only necessary to replace the punching piece without replacing the entire mounting piece and the punching piece, which effectively reduces the production cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] FIG. 1 is a schematic diagram of a culture dish punching device in one embodiment of the present application.

[0018] Among them, the reference numerals are: mounting member 1, punching member 2, fixing portion 21, punching portion 22, operating member 3, pressing portion 31, connecting portion 32, supporting portion 33, anti-slip portion 34, protective member 4, protective space L. DETAILED DESCRIPTION

[0019] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0020] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0021] It is easy to know that there are differences in the size of embryos of different species. For example, the diameter of mouse embryos is about 80 μm, the diameter of monkey embryos is about 120 μm, the diameter of human, dog and pig embryos is about 130 μm, the diameter of sheep embryos is about 150 μm, etc. Of course, the culture of embryos of different species in microwells has different requirements for the preparation of microwells. For example, embryos with small diameters require small-sized microwells for culture, and embryos with large diameters require large-sized microwells for culture. To this end, an embodiment of the present application provides a culture dish punching device, which can prepare microwells of different sizes, thereby adapting to the culture of embryos of different species.

[0022] In actual use, the culture dish punching device acts on the bottom of the culture dish. By pressing, small depressions (micropores) of different sizes with smooth sides and no gaps can be prepared on the bottom of the culture dish. Embryos and cells can gather in the micropores, thereby realizing embryo culture.

[0023] As shown in FIG1 , the culture dish punching device includes a mounting member 1, a punching member 2 and an operating member 3. The mounting member 1 is the main part of the entire culture dish punching device, and the mounting member 1 is used to mount the punching member 2 and the operating member 3; the punching member 2 is arranged at one end of the mounting member 1, and the punching member 2 is used to form / prepare holes on the culture dish; the operating member 3 is arranged at one end of the mounting member 1 away from the punching member 2, and the operating member 3 can drive the mounting member 1 to move under the action of an external force, thereby realizing the punching operation.

[0024] The mounting member 1 is a plate / block / table / column-shaped object with a certain length, height and width, so as to provide an installation position for the punching member 2 and the operating member 3. Furthermore, the mounting member 1 is provided with a mounting groove, and the mounting groove is used to install the punching member 2. Furthermore, the mounting member 1 is provided with a mounting surface at one end away from the mounting groove, and the mounting surface is used to install the operating member 3.

[0025] The punching member 2 can also be called a needle. After the end of the needle pierces the surface of the bottom of the culture dish, a small depression can be formed, thus preparing a micropore. In the embodiment disclosed in the present application, the end of the needle is U-shaped, so that the micropore formed by pressing is U-shaped. The U-shaped micropore is a non-completely curved surface, which is conducive to the culture of embryos.

[0026] The punching member 2 includes a fixing portion 21 and a punching portion 22 connected to each other. The fixing portion 21 is used to connect with the mounting member 1 to achieve the installation of the punching member 2. The punching portion 22 of the punching member 2 contacts the surface of the bottom of the culture dish and can form a hole on the culture dish under the action of manual pressure. Specifically, the fixing portion 21 includes a first end and a second end that are arranged opposite to each other. The first end is accommodated in the mounting groove, and the punching portion 22 is arranged at the second end of the fixing portion 21; it can be understood that the second end and the punching portion 22 are combined to form a U shape.

[0027] The punching piece 2 is provided with a variety of sizes, and the size of each punching piece 2 is different. The punching pieces 2 of different sizes can be replaced relative to the mounting piece 1. By replacing the punching pieces 2 of different sizes on the mounting piece 1, microholes of different sizes can be prepared on the culture dish, which effectively solves the problem of preparing microholes of different sizes for culturing embryos of different species in different scientific research experiments, that is, the culture dish punching device can meet the needs of adapting to the cultivation of different species.

[0028] The punching member 2 is detachably connected to the mounting member 1. Specifically, the first end of the fixing portion 21 can be inserted into the mounting slot of the mounting member 1 and can also be removed from the mounting slot. It can be understood that the first end of the fixing portion 21 remains fixed relative to the mounting member 1 after being inserted into the mounting slot, and the fixing portion 21 will not rotate or move; the fixing and removal from the mounting slot can be achieved manually or with the assistance of additional tools.

[0029] By making the punching member 2 and the mounting member 1 detachable, the punching member 2 of different sizes can be replaced / replaced relative to the mounting member 1. In actual use, the punching member 2 can be replaced according to actual needs. For example, when the punching member 2 needs to be replaced, the current punching member 2 is taken out from the mounting slot, and another punching member 2 of a suitable size is selected and inserted into the mounting slot, and the replacement of the punching member 2 can be completed, thereby preparing micropores of different sizes. When preparing micropores of different sizes, the culture dish punching device of the present application only needs to replace the punching member 2, without having to replace the entire mounting member 1 and the punching member 2, which effectively reduces the production cost.

[0030] When replacing the punching piece 2, it is necessary to insert the first end of the punching portion 22 into the mounting groove. In the embodiment disclosed in the present application, the first end of the fixing portion 21 of each punching piece 2 of different sizes is the same and can match the mounting groove, that is, each punching piece 2 of different sizes can be inserted into the mounting groove.

[0031] For punching pieces 2 of different sizes, the difference lies in the size of the punching portion 22. Specifically, the punching portion 22 is constructed in a spherical shape, and the diameter range of the punching portion 22 is 0.3mm-1mm. Punching pieces 2 of different diameters between 0.3mm-1mm are set. The staff can select the appropriate punching piece 2 for punching according to actual needs, and then prepare microholes of different diameters, which can meet the needs of embryo culture of different species.

[0032] The outer peripheral surface of the fixing portion 21 is provided with a first mark. Specifically, the distance between the first mark and the punching portion 22 is 150 μm-300 μm. The first mark may be a scale ring. The first mark is used to indicate the punching depth of the punching portion 22 to the staff.

[0033] The outer circumference of the first end of the fixing portion 21 is provided with a second mark, and the second mark is used to indicate the diameter of the punching portion 22. Specifically, the first end can be made of a transparent material, so that it is convenient for the staff to see the diameter of the punching portion 22, which is conducive to distinguishing punching members 2 of different sizes. In some embodiments, except for the first end made of a transparent material, the other parts of the punching member 2 can be made of stainless steel.

[0034] The second end of the fixing portion 21 is provided with a receiving groove for installing the punching portion 22. Specifically, the receiving groove matches the punching portion 22, a part of the punching portion 22 is located in the receiving groove, and another part of the punching portion 22 is located outside the receiving groove.

[0035] In other embodiments, the accommodating groove may not be provided, and the fixing portion 21 and the punching portion 22 may be integrally formed.

[0036] The operating member 3 is operated manually or by an external machine. The operating member 3 can drive the mounting member 1 and the punching member 2 to move toward the bottom of the culture dish to achieve the punching operation.

[0037] Taking manual operation as an example, the operating member 3 can be used as a handle, that is, the operating member 3 is manually held / gripped. After the position of the microhole is determined, the mounting member 1 and the punching member 2 are manually driven by the operating member 3 to move to the position where the hole is to be punched on the bottom surface of the culture dish and stop. Then, a pressing force is manually applied to the operating member 3 so that the punching member 2 can be recessed downward from the bottom surface of the culture dish, thereby forming a microhole.

[0038] Taking the operation of an external machine as an example, the external machine clamps the operating part 3, and after determining the position of the microhole, the external machine drives the mounting part 1 and the punching part 2 to move to the bottom surface of the culture dish where the hole is to be punched and stops through the operating part 3. Then the external machine applies pressing pressure to the operating part 3, so that the punching part 2 can be recessed downward from the bottom surface of the culture dish, thereby forming a microhole.

[0039] Preferably, the culture dish punching device of the present application is operated manually by hand.

[0040] The operating member 3 includes a pressing portion 31, a connecting portion 32 and a supporting portion 33. The connecting portion 32 is connected between the pressing portion 31 and the supporting portion 33, the pressing portion 31 is connected to an end of the mounting member 1 away from the punching member 2, the pressing portion 31 is used to abut against a finger, and the supporting portion 33 is used to abut against a palm.

[0041] In the embodiment disclosed in the present application, the pressing portion 31 is configured to be a flat plate, so as to facilitate the placement of fingers and facilitate the fingers to exert force; the supporting portion 33 is configured to be an arc shape, so as to fit the palm of the hand, which is conducive to providing a stable supporting force for the palm, thereby ensuring that the fingers apply pressing pressure to the pressing portion 31 in a stable state, thereby preparing micropores with smooth inner walls.

[0042] In other embodiments, the support portion 33 may not be used to support the palm, but may be used to support the fingers. For example, the thumb and middle finger are placed on the pressing portion 31, and the index finger is placed on the support portion 33. The three thumbs may simultaneously apply pressing force to the pressing portion 31 and the support portion 33 to prepare micropores. In actual use, a suitable operation mode may be selected according to actual conditions.

[0043] In other embodiments, the support portion 33 may also be configured to be spherical.

[0044] The operating member 3 also includes an anti-slip portion 34, which is disposed on the pressing portion 31 and / or the supporting portion 33. In the embodiment disclosed in the present application, the anti-slip portion 34 is a striped structure, and the anti-slip portion 34 can increase the friction between the staff's hand and the operating member 3. When preparing micropores, the anti-slip portion 34 can prevent the staff's hand and the operating member 3 from slipping.

[0045] In other embodiments, the anti-slip portion 34 may be a structure such as an anti-slip sleeve.

[0046] Regarding the operating member 3, in other embodiments, the culture dish punching device may only include the mounting member 1 and the punching member 2 without the operating member 3. In this embodiment, the mounting member 1 not only serves to mount the punching member 2, but also serves as a handle for manual hand-held use.

[0047] The culture dish punching device also includes a protective member 4, which is detachably connected to the mounting member 1 and is used to seal the punching member 2. Specifically, the protective member 4 can be detached from the mounting member 1 to prepare micropores on the culture dish. After the micropores are prepared, the protective member 4 can be reinstalled on the mounting member 1 to seal and store the punching member 2, thereby preventing external dust, debris, etc. from sticking to the punching member 2, thereby protecting the punching member 2.

[0048] The protective member 4 includes an opening and a protective space L that are interconnected. The end of the protective member 4 away from the opening is closed. The opening is used for allowing at least part of the mounting member 1 and the punching member 2 to enter and exit the protective space L. The inner wall of the protective member 4 at the opening can fit with the outer wall of the mounting member 1; when the protective member 4 is not connected to the mounting member 1, the protective space L is connected to the external space through the opening; when the mounting member 1 and the punching member 2 enter the protective space L through the outlet, the protective space is blocked, thereby sealing the punching member 2.

[0049] Furthermore, in the embodiment disclosed in the present application, the protective member 4 is provided with an internal thread on the inner wall circumference at the opening, and the mounting member 1 is provided with an external thread on the outer wall circumference, and the protective member 4 and the mounting member 1 are detachably connected through the internal thread and external thread structure.

[0050] In other embodiments, the protective component 4 and the mounting component 1 may be detachably connected by means of structures such as snaps and magnets.

[0051] Microwells were prepared in a cell culture dish by the culture dish punching device of the present application, and then two groups of mouse embryo culture experiments were carried out. The experimental process was to place the two groups of embryos in the two groups of microwells prepared by the culture dish punching device of the present application for embryo culture to form blastocysts. The specific experimental data were as follows:

[0052] Group 1:

[0053]

[0054] Group 2:

[0055]

[0056] After the KSOM culture test, the average blastocyst development rate of the experimental group in microwell culture was higher than that of the control group, and the abnormality rate was significantly lower than that of the control group, that is, the microwells prepared by the culture dish punching device of the present application are more conducive to embryo culture.

[0057] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0058] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0059] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0060] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0061] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0062] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. A culture dish punching device, characterized in that: include: A mounting member provided with a mounting groove; and a punching member, detachably connected to the mounting member, the punching member comprising a fixing portion and a punching portion connected to each other, the fixing portion comprising a first end and a second end arranged opposite to each other, the first end being accommodated in the mounting groove, the second end being connected to the punching portion, and the punching portion being used to form a hole on the culture dish; Wherein, punching pieces of different sizes can be replaced relative to the mounting piece.

2. The culture dish punching device according to claim 1, characterized in that: The punching portion is spherical in shape, and the diameter of the punching portion is in the range of 0.3 mm to 1 mm.

3. The culture dish punching device according to claim 1, characterized in that: A first mark is provided on the outer peripheral surface of the fixing portion, and the first mark is used to indicate the punching depth of the punching portion.

4. The culture dish punching device according to claim 1, characterized in that: A second mark is provided on the outer peripheral surface of the first end, and the second mark is used to indicate the diameter size of the punching portion.

5. The culture dish punching device according to claim 1, characterized in that: The second end is provided with a receiving groove, and the receiving groove is used for installing the punching part.

6. The culture dish punching device according to claim 1, characterized in that: The culture dish punching device also includes a protective member, which is detachably connected to the mounting member and is used to seal the punching member.

7. The culture dish punching device according to claim 6, characterized in that: The protective member comprises an opening and a protective space which are interconnected, wherein the opening is used for allowing at least part of the mounting member and the punching member to enter and exit the protective space, and the inner wall of the protective member at the opening can fit with the outer wall of the mounting member.

8. The culture dish punching device according to any one of claims 1 to 7, characterized in that: The culture dish punching device also includes an operating member, which is arranged at one end of the mounting member away from the punching member, and the operating member can drive the mounting member to move under the action of an external force.

9. The culture dish punching device according to claim 8, characterized in that: The operating member includes a pressing portion, a connecting portion and a supporting portion, wherein the connecting portion is connected between the pressing portion and the supporting portion, the pressing portion is connected to the mounting member, the pressing portion is used to abut against a finger, and the supporting portion is used to abut against a finger or a palm.

10. The culture dish punching device according to claim 9, characterized in that: The operating member further includes an anti-slip portion, and the anti-slip portion is arranged on the pressing portion and / or the supporting portion.

Images