Cell culture device

Abstract

The utility model provides a cell culture device which comprises a placing box, a fixed heat preservation part is fixedly connected in the placing box, and a plurality of clamping grooves for placing test tubes are formed in the fixed heat preservation part; a plurality of openings are formed in the fixed thermal insulation part, and movable thermal insulation parts are slidably arranged at the openings and connected with driving mechanisms; a plurality of observation openings are formed in the side wall of the containing box and formed in one side of the opening. The device is convenient to operate, has a good preservation effect on the test tube, has a damping function, reduces damage of external factors to the test tube, has a heat preservation function, does not influence observation of bacterial culture in the test tube, can provide a dark environment for part of special bacteria, and is beneficial to bacterial culture work.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device. Background Art

[0002] Cells are divided into prokaryotic cells and eukaryotic cells. In cell culture, the most common is microbial culture and strain preservation. Microorganisms are a large group of organisms including bacteria, viruses, fungi, and some small protozoa. Common bacteria are a type of prokaryotic cell. In culture, it is often necessary to prepare test tube slant culture media for bacterial growth and strain preservation. Slant culture media is a form of solid culture media; when making it, it should be quantitatively dispensed into test tubes while hot, and solidified into a slant, which is called a slant culture media, which is used for strain expansion, transfer to tubes, and strain preservation.

[0003] Patent application number 201920492984.8 discloses a test tube rack for sterilizing test tube culture medium, including an annular support on the upper part of the test tube, a connecting rod and an annular tray on the bottom of the test tube. The annular support on the upper part of the test tube is composed of 7 circular rings connected to each other, and the connecting rod is composed of 7 steel bars. The upper part of the 7 steel bars is connected to the upper annular support of the test tube and the lower part is connected to the annular tray on the bottom of the test tube. The annular tray on the bottom of the test tube is composed of 7 hollow circular trays connected to each other. However, the device still has the following problems:

[0004] 1. The exposure of the test tube culture medium is not conducive to heat preservation. If the outer layer is wrapped with thermal insulation cotton, it is conducive to the heat preservation work, but it is not conducive to observing the internal state. At the same time, there is no way to provide a dark environment for special fungi, which is not conducive to bacterial culture.

[0005] 2. Since the culture medium in the test tube is unbalanced and easy to shake, it may be accidentally knocked over or even broken. It is easily disturbed by the external environment and can easily break. Utility Model Content

[0006] In order to solve the problems existing in the above-mentioned prior art, a cell culture device is provided. The device is easy to operate, can have a good preservation effect on the test tube, has a shock-absorbing function, reduces the damage to the test tube caused by external factors, has a heat preservation function, and does not affect the observation of the bacterial culture in the test tube. In addition, it can provide a dark environment for some special fungi, which is conducive to the bacterial culture work.

[0007] The technical solution adopted by the utility model to solve its technical problems is:

[0008] The utility model proposes a cell culture device, comprising a placement box, wherein a fixed thermal insulation is fixedly connected inside the placement box, and the fixed thermal insulation is provided with a plurality of clamping grooves for placing test tubes; the fixed thermal insulation is provided with a plurality of openings, and movable thermal insulation is slidably provided at the openings, and the movable thermal insulation is connected with a driving mechanism; and a plurality of observation ports are provided on a side wall of the placement box, and the observation ports are arranged on one side of the opening.

[0009] Preferably, the observation port includes an upper observation port and a lower observation port.

[0010] Preferably, the driving mechanism includes a plurality of moving rods, the number of the moving rods is the same as the number of the movable thermal insulation, each moving rod is hinged to the corresponding movable thermal insulation, and adjacent moving rods are connected by connecting rods.

[0011] Preferably, the placement box is slidably connected to a guide rod that moves in a vertical direction, the guide rod and the connecting rod are fixedly connected, and the moving rod and the connecting rod are hinged.

[0012] Preferably, a slide rail is fixedly connected inside the placement box, a slider is slidably connected inside the slide rail, and the slider is hinged to the moving rod.

[0013] Preferably, the slide rail includes a horizontal section and a vertical section, the horizontal section and the vertical section are connected to each other, and the vertical section is located above the corresponding observation port.

[0014] Preferably, the placement box is threadedly connected to an upper cover insulation, the upper cover insulation is located above the fixed insulation, and a cavity for accommodating a test tube is opened in the upper cover insulation.

[0015] Preferably, a movable groove is further provided below the fixed heat-insulating member, and the movable groove is communicated with the clamping groove.

[0016] Preferably, a movable seat that moves vertically is slidably connected in the movable groove, the lower end of the movable seat is connected to the placement box through a shock-absorbing spring, and the upper end of the movable seat is provided with a groove for placing the test tube; the width of the movable seat is smaller than the aperture of the clamping groove.

[0017] Preferably, one side of the placement box is rotatably connected to a nut, the nut is threadedly connected to a screw rod, and the screw rod is slidably connected to the placement box; the other side of the placement box is rotatably connected to a support rod.

[0018] Compared with the prior art, the beneficial effects of the utility model are:

[0019] 1. The utility model is provided with movable thermal insulation, which cooperates with fixed thermal insulation to realize thermal insulation of test tubes. When observation is needed, the guide rod can drive the movable rod to move, and under the limiting effect of the slide rail, the movable thermal insulation can be driven to move, so that the opening of the fixed thermal insulation is opened, and the operator can observe the test tube situation through the observation port more conveniently, while not affecting the normal progress of the thermal insulation work, reducing the impact on the thermal insulation effect, and facilitating the bacterial culture work in the test tube.

[0020] 2. The utility model is also provided with a movable seat. Under the action of the shock-absorbing spring, the movable seat can move in the movable groove. The movable seat is used for supporting the bottom of the test tube, which can have a good shock-absorbing effect on the test tube, effectively reduce the occurrence of test tube breakage due to external factors, and effectively improve the preservation effect of the test tube, so as not to affect the progress of bacterial culture.

[0021] 3. The utility model is also provided with a nut, by rotating the nut, the screw can be driven to extend, at this time the screw can make the placement box tilted at a suitable angle, and the support rod provided at the same time can use the placement box as a whole to support the work, so that the degree of inclination of the test tube can be automatically adjusted according to actual needs, thereby facilitating the bacterial culture work and effectively increasing the bacterial culture area. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0023] Figure 1 It is an overall stereogram of the utility model;

[0024] Figure 2 It is an overall top view of the utility model;

[0025] Figure 3 yes Figure 2 Schematic diagram of the structure of the AA part;

[0026] Figure 4 yes Figure 2 Schematic diagram of the AA part structure (in working state).

[0027] Description of reference numerals:

[0028] 1. Placement box; 2. Upper cover insulation; 3. Push-pull block; 4. Upper observation port; 5. Lower observation port; 6. Handle; 7. Moving rod; 8. Movable insulation; 9. Slide rail; 10. Guide rod; 11. Fixed insulation; 12. Test tube; 13. Connecting rod; 14. Clamping groove; 15. Movable groove; 16. Movable seat; 17. Shock-absorbing spring; 18. Nut; 19. Screw rod; 20. Support rod. DETAILED DESCRIPTION

[0029] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0030] Embodiment 1

[0031] like Figure 1-4 As shown, this embodiment proposes a cell culture device, including a placement box 1, in which a fixed insulation 11 is fixedly connected, and the fixed insulation 11 is provided with a plurality of clamping grooves 14 for placing a test tube 12. The clamping grooves 14 can be connected with insulation materials such as insulation cotton, which can improve the insulation effect on the one hand, and on the other hand, the flexible insulation material can protect the test tube to a certain extent, effectively reducing the occurrence of test tube breakage.

[0032] The side wall of the fixed insulation 11 is provided with a plurality of openings, at which the movable insulation 8 is slidably arranged, and the movable insulation 8 is connected with a driving mechanism; the side wall of the placement box 1 is provided with a plurality of observation ports, which are arranged at one side of the opening.

[0033] The observation port includes an upper observation port 4 and a lower observation port 5. The upper observation port 4 is located above the lower observation port 5. The upper observation port 4 and the lower observation port 5 are respectively used for observing the upper and lower parts of the test tube 12. The number of observation ports and the number of openings are adapted to each other, and one opening corresponds to one observation port.

[0034] The upper observation port 4 and the lower observation port 5 are both made of transparent glass, so as to facilitate observation of the reaction of the test tube 12. The rest of the placing box 1 can be made of opaque material, and can also provide a dark environment for some special fungi, which is conducive to the bacterial culture work. Only when the upper observation port 4 and the lower observation port 5 are opened, the situation in the test tube 12 can be seen.

[0035] The driving mechanism is used to drive the movable insulation 8 to move. In the initial state, the movable insulation 8 is located in the opening. The movable insulation 8 and the fixed insulation 11 cooperate with each other to achieve the insulation work of the test tube 12. A block can be set in the opening.

[0036] The stopper limits the movement of the movable thermal insulation 8 to prevent the movable thermal insulation 8 from moving too far into the opening. When the movable thermal insulation 8 is located in the opening, the inner wall of the movable thermal insulation 8 and the inner wall of the fixed thermal insulation 11 form a plane, thereby facilitating the clamping of the test tube 12.

[0037] The driving mechanism includes a plurality of moving rods 7 , the number of the moving rods 7 is the same as the number of the movable heat insulation 8 , each moving rod 7 is hinged to the corresponding movable heat insulation 8 , and adjacent moving rods 7 are connected via a connecting rod 13 .

[0038] The placement box 1 is slidably connected to a guide rod 10 that moves in the vertical direction. The guide rod 10 and the connecting rod 13 are fixedly connected. The movable rod 7 and the connecting rod 13 are hinged. The upper end of the guide rod 10 passes through the placement box 1, and the guide rod 10 can only slide in the vertical direction.

[0039] A slide rail 9 is fixedly connected to the placement box 1, and a slider is slidably connected to the slide rail 9. The slider and the moving rod 7 are hinged. The slide rail 9 limits and guides the movement of the moving rod 7. When the moving rod 7 is moved by pulling the guide rod 10, the slider can be driven to slide in the slide rail 9, and the slider and the moving rod 7 can rotate relative to each other.

[0040] The slide rail 9 includes a horizontal section and a vertical section, the horizontal section and the vertical section are connected to each other, the vertical section is located above the corresponding observation port, and the length of the vertical section is long enough. When the guide rod 10 moves to the uppermost end, the connecting rod 13 is in contact with the upper wall of the placement box 1, and the guide rod 10 cannot drive the connecting rod 13 to continue to move upward. At this time, the active insulation 8 is just moved to the top of the corresponding observation port, thereby preventing the active insulation 8 from affecting the line of sight.

[0041] The placing box 1 is threadedly connected with an upper cover insulation 2, which is located above the fixed insulation 11. A cavity that can be used to accommodate a test tube 12 is opened in the upper cover insulation 2. A handle 6 is fixedly connected to the upper end of the upper cover insulation 2. The handle 6 can be used to easily drive the upper cover insulation 2 to rotate, so as to open the upper cover insulation 2. The width of the cavity is suitable enough to facilitate the accommodation of the test tube 12.

[0042] A nut 18 is also rotatably connected to one side of the placement box 1, and a screw 19 is threadedly connected to the nut 18. The screw 19 is slidably connected to the placement box 1. A sliding hole is provided in the placement box 1, and one end of the screw 19 is located in the sliding hole. The screw 19 can be fixedly connected to a telescopic rod, and the other end of the telescopic rod is connected to the placement box 1. The relative sliding of the screw 19 and the placement box 1 can be achieved through the provision of the telescopic rod. When the nut 18 is rotated, the screw 19 can be driven to extend, thereby adjusting the inclination angle of the placement box 1.

[0043] The other side of the placement box 1 is also rotatably connected to a support rod 20 via a rotating shaft. The placement box 1 is rotatably connected to the rotating shaft, and the support rod 20 is fixedly connected to the rotating shaft. The rotating shaft can also be threadedly connected with a locking nut. By reasonably adjusting the angle of the placement box 1, the locking nut can be loosened. At this time, the rotating shaft can rotate around the placement box 1, so that the support rod 20 can contact the plane, and then the locking nut can be tightened. At this time, the angle of the support rod 20 can be fixed, which is convenient for the overall support work of the placement box 1.

[0044] Embodiment 2

[0045] Reference Figure 1-4, other structures are the same as those in the first embodiment, except that the shock absorption problem of the test tube is taken into consideration in this embodiment.

[0046] A movable groove 15 is further provided below the fixed heat-insulating member 11 . The movable groove 15 is connected to the clamping groove 14 . The width of the movable groove 15 is greater than the width of the clamping groove 14 .

[0047] A movable seat 16 that moves in the vertical direction is slidably connected in the movable groove 15, the lower end of the movable seat 16 is connected to the placement box 1 through a shock-absorbing spring 17, and the upper end of the movable seat 16 is provided with a groove for placing the test tube 12. The width of the movable seat 16 is smaller than the aperture of the clamping groove 14, so the movable seat 16 can only move in the movable groove 15, and will not move into the clamping groove 14.

[0048] The upper end of the shock-absorbing spring 17 is connected to the movable seat 16, and the lower end of the shock-absorbing spring 17 is connected to the placement box 1. The shock-absorbing spring 17 can be connected to a damper, which can provide resistance to movement and improve the protection effect on the test tube 12. In the initial state, the shock-absorbing spring 17 is in a compressed state, and the upper end of the movable seat 16 is in contact with the bottom end of the clamping groove 14.

[0049] The specific working process is as follows:

[0050] 1. Prepare the solution: add part of the required amount of water to the test tube 12, weigh various raw materials according to the formula of the culture medium, add them in sequence to dissolve them, and finally add the required water. For substances such as peptone and meat extract, heating is required to dissolve. The water evaporated during the heating process should be added with water after all the raw materials are dissolved.

[0051] 2. Adjust the pH value: Use pH test paper (or pH potentiometer, hydrogen ion concentration colorimeter) to test the pH value of test tube 12. If it does not meet the requirements, it can be adjusted with 10% HCl or 10% NaOH until it is adjusted to the pH value required by the formula.

[0052] 3. Filtration: Use filter paper, gauze or cotton to filter the prepared culture medium while it is still hot. When filtering with gauze, it is best to fold it into six layers. When filtering with filter paper, fold the filter paper into a tile shape and spread it on the funnel for filtration.

[0053] 4. Packaging: The filtered culture medium should be packaged. When making the slant culture medium, the culture medium should be packaged into the test tube 12. When packaging, loosen the spring clamp with one hand to let the culture medium flow out, and hold the test tube 12 with the other hand to collect the culture medium in turn. When packaging, be careful not to let the culture medium adhere to the tube mouth to avoid soaking the cotton plug and causing contamination by bacteria.

[0054] The amount of culture medium loaded into the test tube 12 depends on the size of the test tube and the need. Each 15x150 mm test tube can be used, and about 3 to 4 ml (1 / 4 to 1 / 3 of the test tube height) can be loaded. If a deep culture medium is prepared, each 20x220 mm test tube can be loaded with about 12 to 15 ml.

[0055] 5. Add cotton plug: After the dispensing is completed, the mouth of the test tube 12 needs to be blocked with cotton plug. The main purpose of blocking the cotton plug is to filter the air and avoid pollution. The cotton plug should be made of ordinary fresh and dry cotton. Do not use absorbent cotton, so as to avoid the cotton plug being unusable due to water absorption by the absorbent cotton.

[0056] When making a tampon, spread the cotton to an appropriate thickness according to the size of the tampon, grab a piece the size of the palm of your hand, and spread it in the round hole formed by the thumb and index finger of your left hand. Insert the index finger of your right hand into the middle of the cotton. At the same time, slightly tighten the index finger and thumb of your left hand to form a long stick-shaped tampon.

[0057] After the cotton plug is made, it should be quickly inserted into the test tube 12. The cotton plug should be close to the inner wall without leaving any gaps to prevent airborne microorganisms from invading along the wrinkles. The cotton plug should not be too tight or too loose. After plugging, the cotton plug should be carried by hand without the test tube 12 falling. 2 / 3 of the cotton plug should be in the test tube 12, with a little cotton exposed at the top for easy removal. The test tube with cotton plug should be covered with thick paper and tied with rope for sterilization.

[0058] Then, by rotating the nut 18, the screw rod 19 is moved out to a sufficient length, thereby adjusting the tilt angle of the placement box 1 at this time, and then by loosening the fastening nut, the support rod 20 is rotated, the support rod 20 contacts the plane again, and the fastening nut is tightened to achieve the relative fixation of the angle of the support rod 20, so that the test tube 12 becomes tilted, which is beneficial to the cultivation of bacteria.

[0059] The handle 6 is used to drive the upper insulation cover 2 to rotate, so that the fixed insulation 11 is opened, and the test tube 12 that needs insulation is placed in the clamping groove 14, and then the upper insulation cover 2 is tightened to achieve the placement of the test tube 12. At this time, the lower end of the test tube 12 is located in the groove of the movable seat 16.

[0060] When it is necessary to observe the bacteria in the test tube 12, the guide rod 10 is pulled up, and the guide rod 10 drives the connecting rod 13 to move upward, thereby driving the moving rod 7 to move, and the moving rod 7 drives the slider to move in the slide rail 9. The slide rail 9 guides the movement of the moving rod 7, and the moving rod 7 drives the movable insulation 8 to move.

[0061] When the guide rod 10 moves to the uppermost end, the connecting rod 13 is in contact with the upper wall of the placement box 1, and the guide rod 10 cannot drive the connecting rod 13 to continue to move upward. At this time, the active thermal insulation 8 is just moved to the top of the corresponding observation port, thereby preventing the active thermal insulation 8 from affecting the line of sight. At this time, the situation of the test tube 12 can be observed through the upper observation port 4 and the lower observation port 5. While having the insulation function, it does not affect the observation of the bacterial culture in the test tube 12. In addition, it can provide a dark environment for some special fungi, which is conducive to the bacterial culture work.

[0062] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A cell culture device, comprising a placement box (1), characterized in that: A fixed heat preservation device (11) is fixedly connected inside the placement box (1), and the fixed heat preservation device (11) is provided with a plurality of clamping grooves (14) for placing test tubes (12); the fixed heat preservation device (11) is provided with a plurality of openings, and movable heat preservation devices (8) are slidably provided at the openings, and the movable heat preservation device (8) is connected to a driving mechanism; a plurality of observation ports are provided on a side wall of the placement box (1), and the observation ports are provided on one side of the openings.

2. A cell culture device according to claim 1, characterized in that: The observation port comprises an upper observation port (4) and a lower observation port (5).

3. A cell culture device according to claim 1, characterized in that: The driving mechanism comprises a plurality of moving rods (7), the number of the moving rods (7) being the same as the number of the movable heat insulation (8), each moving rod (7) being hinged to a corresponding movable heat insulation (8), and adjacent moving rods (7) being connected via a connecting rod (13).

4. A cell culture device according to claim 3, characterized in that: The placement box (1) is slidably connected to a guide rod (10) that moves in a vertical direction; the guide rod (10) and the connecting rod (13) are fixedly connected; and the moving rod (7) and the connecting rod (13) are hinged.

5. A cell culture device according to claim 3, characterized in that: A slide rail (9) is fixedly connected inside the placement box (1), a slider is slidably connected inside the slide rail (9), and the slider is hinged to the moving rod (7).

6. A cell culture device according to claim 5, characterized in that: The slide rail (9) comprises a horizontal section and a vertical section, the horizontal section and the vertical section are connected to each other, and the vertical section is located above the corresponding observation port.

7. A cell culture device according to claim 1, characterized in that: The placement box (1) is threadedly connected to an upper insulation cover (2), the upper insulation cover (2) is located above the fixed insulation cover (11), and a cavity for accommodating a test tube (12) is provided in the upper insulation cover (2).

8. The cell culture device according to claim 1, characterized in that: A movable groove (15) is also provided below the fixed heat-insulating member (11), and the movable groove (15) is connected to the clamping groove (14).

9. A cell culture device according to claim 8, characterized in that: A movable seat (16) that moves in a vertical direction is slidably connected in the movable groove (15); the lower end of the movable seat (16) is connected to the placement box (1) via a shock-absorbing spring (17); and the upper end of the movable seat (16) is provided with a groove for placing the test tube (12); the width of the movable seat (16) is smaller than the aperture of the clamping groove (14).

10. The cell culture device according to claim 1, characterized in that: One side of the placement box (1) is also rotatably connected to a nut (18), the nut (18) is threadedly connected to a screw rod (19), and the screw rod (19) and the placement box (1) are slidably connected; the other side of the placement box (1) is also rotatably connected to a support rod (20).

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