Novel embryo culture dish

Abstract

The utility model discloses a novel embryo culture dish which comprises an outer dish, an inner dish is clamped in the outer dish, two sides of the outer part of the inner dish are connected with first stop blocks, a dish cover is arranged above the inner dish, a second stop block is arranged at the lower end of the dish cover, and a supporting plate is arranged at the bottom of the outer dish. The bottom of the outer vessel is divided into a first containing chamber and a second containing chamber by the supporting plate, the inner vessel comprises a cleaning cavity and a culture cavity, a hole is formed in the bottom of the cleaning cavity, a culture unit is arranged in the culture cavity and comprises a culture groove, a trapezoidal groove and a liquid outlet pipe, a waste liquid pipe is arranged on the side wall of the inner vessel, and the tail end of the waste liquid pipe is connected with a waste liquid box. A first air bag is arranged on one side of the waste liquid box, a liquid storage box is connected to the upper portion of the dish cover and connected with a liquid inlet pipe, and a second air bag is arranged at the top of the liquid inlet pipe. By adopting the technical scheme provided by the utility model, the requirements of people on conveniently replacing a culture solution and conveniently observing and selecting a single embryo can be met.

Description

Technical Field

[0001] The utility model relates to the technical field of culture dishes, in particular to a novel embryo culture. Background Art

[0002] Artificial assisted reproductive technology (abbreviated as "assisted reproductive technology") refers to the technology of using medical assistance to make infertile couples pregnant. The most widely used one is in vitro fertilization-embryo transfer technology and its derivative technologies, which is commonly known as test tube babies, that is, the fertilization and embryo cultivation stages will be carried out in glassware, and the embryo and fetal development stages will be carried out in the mother. In order to improve the success rate of pregnancy, multiple embryos are generally implanted into the mother, but this may cause multiple pregnancies, and complications of multiple pregnancies are more common than single pregnancies. Therefore, evaluating the embryos and selecting the embryo with the best developmental potential from multiple embryos for transplantation, and ensuring the success rate while implementing single embryo transplantation is the true meaning of assisted reproduction. A large number of literatures show that embryo collective culture is conducive to the formation of blastocysts. Embryo collective culture refers to culturing multiple embryos together in a single culture drop. The growth factors and other substances secreted by the embryos promote the growth and development of adjacent embryos. The blastocysts obtained by this embryo collective culture method are of better quality and have a higher survival rate.

[0003] The existing new embryo culture dishes have the following problems:

[0004] 1. Different stages of embryo cultivation require different nutrient solutions, and the process of changing the nutrient solution is too cumbersome;

[0005] 2. It is impossible to evaluate individual embryos during embryo collection culture, making selection inconvenient. Utility Model Content

[0006] The utility model aims to provide a novel embryo culture dish to solve the problems existing in the existing novel embryo culture dishes.

[0007] A novel embryo culture dish comprises an outer dish, wherein an inner dish is arranged inside the outer dish, first blocks are connected to both sides of the outer side of the inner dish, a dish cover is arranged above the inner dish, a second block is arranged at the lower end of the dish cover, a support plate is arranged at the bottom of the outer dish, the support plate divides the bottom of the outer dish into a first receiving chamber and a second receiving chamber, the inner dish comprises a cleaning chamber and a culture chamber, a hole is arranged at the bottom of the cleaning chamber, a culture unit is arranged in the culture chamber, the culture unit comprises a culture trough, a trapezoidal trough and a liquid outlet pipe, a waste liquid pipe is arranged on the side wall of the inner dish, a waste liquid tank is connected to the end of the waste liquid pipe, a first air bag is arranged on one side of the waste liquid tank, a liquid storage tank is connected above the dish cover, a liquid storage tank is connected to a liquid inlet pipe, and a second air bag is arranged on the top of the liquid inlet pipe.

[0008] Preferably, a liquid container is provided in the first containing chamber, a first liquid drain pipe is provided on the top of the liquid container, the first liquid drain pipe is connected to the hole, a second liquid drain pipe is provided at the left end of the liquid container, and the end of the second liquid drain pipe passes through the outer dish.

[0009] Preferably, the culture tank is enclosed by a perforated plate.

[0010] Preferably, the liquid outlet tube is connected to the culture chamber and the second containing chamber.

[0011] Preferably, the diameter of the liquid outlet tube is smaller than the diameter of the embryo.

[0012] Preferably, a valve is provided on the second liquid discharge pipe.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. Use the siphon effect to easily replace the culture medium;

[0015] 2. It can take advantage of the collective culture to culture embryos, and can use the structure of the culture unit to quickly take out individual embryos for easy selection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic diagram of the structure of a novel embryo culture dish provided in an embodiment of the utility model;

[0017] Figure 2 A top view of the inner container provided in an embodiment of the utility model;

[0018] Figure 3 A side view of a culture unit provided in an embodiment of the utility model. DETAILED DESCRIPTION

[0019] The present invention is further described in detail below in conjunction with the accompanying drawings and implementation examples:

[0020] The figure marks in the drawings of the specification include: 1. outer dish; 2. inner dish; 3. first stopper; 4. dish cover; 5. second stopper; 6. support plate; 7. first holding chamber; 8. second holding chamber; 9. cleaning chamber; 10. culture chamber; 11. hole; 12. culture tank; 13. trapezoidal groove; 14. liquid outlet pipe; 15. waste liquid pipe; 16. waste liquid tank; 17. first air bag; 18. liquid storage tank; 19. liquid inlet pipe; 20. second air bag; 21. liquid container; 22. first liquid discharge pipe; 23. second liquid discharge pipe; 24. perforated plate; 25. valve.

[0021] like Figure 1 , Figure 2As shown, a novel embryo culture dish comprises an outer dish 1, an inner dish 2 is arranged inside the outer dish 1, first blocks 3 are connected to the outer sides of the inner dish 2, a dish cover 4 is arranged above the inner dish 2, and a second block 5 is arranged at the lower end of the dish cover 4. A support plate 6 is arranged at the bottom of the outer dish 1, and the support plate 6 divides the bottom of the outer dish 1 into a first receiving chamber 7 and a second receiving chamber 8. A liquid container 21 is arranged in the first receiving chamber 7, and a first liquid discharge pipe 22 is arranged at the top of the liquid container 21. The first liquid discharge pipe 22 is connected to the hole 11. A second liquid discharge pipe 23 is arranged at the left end of the liquid container 21, and the end of the second liquid discharge pipe 23 passes through the outer dish 1. A valve 25 is arranged on the second liquid discharge pipe 23. The inner dish 2 comprises a cleaning chamber 9 and a culture chamber 10. A hole 11 is arranged at the bottom of the cleaning chamber 9, and a culture unit is arranged in the culture chamber 10. The culture unit comprises a culture tank 12, a trapezoidal tank 13 and a liquid outlet pipe 14. The liquid outlet pipe 14 is connected to the culture chamber 10 and the second receiving chamber 8. The diameter of the liquid outlet pipe 14 is smaller than the diameter of the embryo. The embryo will not enter the second receiving chamber 8 from the liquid outlet pipe 14, but will be fixed in the culture unit. A waste liquid pipe 15 is provided on the side wall of the inner dish 2. A waste liquid tank 16 is connected to the end of the waste liquid pipe 15. A first air bag 17 is provided on one side of the waste liquid tank 16. A liquid storage tank 18 is connected to the top of the dish cover 4. The liquid storage tank 18 is connected to a liquid inlet pipe 19. A second air bag 20 is provided on the top of the liquid inlet pipe 19.

[0022] like Figure 3 As shown, the culture tank 12 is surrounded by a plate with holes 11, and adjacent culture units can use the hollow plates to achieve the exchange of growth factors and other substances.

[0023] The specific implementation process is as follows:

[0024] When in use, the direction of the inner dish 2 is adjusted according to the position of the first liquid discharge pipe 22, and the inner dish 2 is clamped on the outer dish 1 by the first stopper 3, and the culture unit is placed in the inner dish 2 to complete the basic assembly. The embryo is placed in the culture unit to facilitate the observation of the growth and development of a single embryo. The culture unit including the embryo can also be directly taken out according to the actual situation to avoid picking them up one by one. The dish cover 4 is clamped on the top of the inner dish 2 by the second stopper 5 to complete the overall fixation.

[0025] The culture fluid required for culture is injected into the liquid storage tank 18, and the second air bag 20 is squeezed so that the culture fluid in the liquid storage tank 18 is injected into the culture chamber 10 through the liquid inlet pipe 19 under the action of the siphon effect, and the height of the culture fluid in the culture chamber 10 is observed. When it reaches a certain height, the injection of the culture fluid is stopped; when the fluid needs to be replaced, the first air bag 17 is squeezed, and the waste fluid to be discharged enters the waste liquid tank 16 through the waste liquid pipe 15 under the action of the siphon effect.

[0026] By adjusting the opening and closing of the valve on the second liquid discharge pipe 23 , the liquid in the cleaning chamber 9 can be replaced conveniently.

[0027] The above is only an embodiment of the utility model, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the utility model, several deformations and improvements can be made, which should also be regarded as the protection scope of the utility model, and these will not affect the effect of the implementation of the utility model and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A novel embryo culture dish, characterized in that: The invention comprises an outer dish (1), wherein an inner dish (2) is arranged inside the outer dish (1), first stoppers (3) are connected to both sides of the outer side of the inner dish (2), a dish cover (4) is arranged above the inner dish (2), a second stopper (5) is arranged at the lower end of the dish cover (4), a support plate (6) is arranged at the bottom of the outer dish (1), and the support plate (6) divides the bottom of the outer dish (1) into a first receiving chamber (7) and a second receiving chamber (8), and the inner dish (2) comprises a cleaning chamber (9) and a culture chamber (10), and the bottom of the cleaning chamber (9) is provided with a hole ( 11), a culture unit is arranged in the culture chamber (10), and the culture unit includes a culture trough (12), a trapezoidal trough (13) and a liquid outlet pipe (14); a waste liquid pipe (15) is arranged on the side wall of the inner dish (2); the end of the waste liquid pipe (15) is connected to a waste liquid tank (16); a first air bag (17) is arranged on one side of the waste liquid tank (16); a liquid storage tank (18) is connected to the top of the dish cover (4); the liquid storage tank (18) is connected to a liquid inlet pipe (19); a second air bag (20) is arranged on the top of the liquid inlet pipe (19).

2. The novel embryo culture dish according to claim 1, characterized in that: A liquid container (21) is arranged in the first containing chamber (7), a first liquid drain pipe (22) is arranged on the top of the liquid container (21), the first liquid drain pipe (22) is connected to the hole (11), a second liquid drain pipe (23) is arranged at the left end of the liquid container (21), and the end of the second liquid drain pipe (23) passes through the outer dish (1).

3. The novel embryo culture dish according to claim 1, characterized in that: The culture tank (12) is enclosed by a plate with holes (11).

4. The novel embryo culture dish according to claim 1, characterized in that: The liquid outlet tube (14) is connected to the culture chamber (10) and the second containing chamber (8).

5. The novel embryo culture dish according to claim 4, characterized in that: The diameter of the liquid outlet tube (14) is smaller than the diameter of the embryo.

6. The novel embryo culture dish according to claim 2, characterized in that: The second liquid discharge pipe (23) is provided with a valve (25).

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