Cell culture bottle for culturing umbilical cord stem cells

Abstract

The utility model provides a cell culture bottle for culturing umbilical cord stem cells, which relates to the technical field of cell culture bottles, and comprises a bottle body, a liquid inlet hole and a liquid outlet hole, one end of the bottle opening assembly covers the liquid inlet hole and is connected with the bottle body; one end of the pressing lock assembly is connected with the bottle opening assembly; and the sealing buckle assembly is arranged on the pressing lock assembly. The bottle opening assembly can be sealed through mutual matching of the pressing lock assembly and the sealing buckle assembly, then the experiment success rate of a user can be improved, meanwhile, the pressing lock assembly and the sealing buckle assembly can be removed from the bottle opening assembly by pressing the pressing lock assembly, and the experiment efficiency is improved. Therefore, when the internal and external air pressure difference inside and outside the bottle is too large, the pressing lock assembly and the sealing buckle assembly cannot be separated from the bottle opening assembly, and a user can use the bottle conveniently.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture bottles, in particular to a cell culture bottle for culturing umbilical cord stem cells. Background Art

[0002] Cell culture flasks are important equipment for drug testing and implantable medical device testing. They are highly airtight and safe, and are therefore widely favored by researchers in the pharmaceutical and medical device industries. Cell culture flasks can be divided into glass cell culture flasks and plastic cell culture flasks based on their materials. The shape of the bottom of the cell culture flask can be round, square, rectangular, triangular, etc. The volume of the cell culture flask has a variety of specifications ranging from 500ml to 3L.

[0003] At present, the cell culture flask on the market includes a bottle body and a bottle cap detachably connected to the bottle body. The traditional cell culture flask is sealed by rotating the bottle cap, which is relatively troublesome to operate.

[0004] In view of this, this application is hereby filed. Utility Model Content

[0005] The utility model provides a cell culture bottle for culturing umbilical cord stem cells. The device can be well sealed and easily released through the cooperation between a pressing lock component and a sealing buckle component, and the operation is simple.

[0006] In order to solve the above technical problems, the technical solution of the utility model is as follows:

[0007] The embodiment of the utility model provides a cell culture bottle for culturing umbilical cord stem cells, comprising:

[0008] A bottle body, wherein a liquid inlet hole is formed on the bottle body;

[0009] A bottle mouth assembly, one end of which covers the liquid inlet hole and is connected to the bottle body;

[0010] Press the lock assembly to connect the bottle mouth assembly at one end;

[0011] The sealing buckle assembly is arranged on the push lock assembly.

[0012] Furthermore, the bottle mouth assembly includes:

[0013] A connecting tube, one end of which covers the liquid inlet hole;

[0014] A square pipe fitting, one end of which is fixedly connected to the other end of the connecting pipe.

[0015] Furthermore, the square tube includes:

[0016] A square tube, one end of which is fixedly connected to the other end of the connecting tube, and the other end of which is connected to the push lock assembly;

[0017] A sealing rubber ring is sleeved on one end of the square tube and fixedly connected to the outer wall of the square tube;

[0018] The first sealing base is sleeved on the other end of the square tube, and the inner wall is fixedly connected to the outer wall of the square tube;

[0019] The bottom of the sealing ring is fixedly connected to the top surface of the first sealing base and the other end of the square tube.

[0020] Furthermore, the push lock assembly includes:

[0021] A bottle cover plate, covering the other end of the square tube and abutting against the sealing ring;

[0022] A bottle cap ring, the top of which is fixedly connected to the bottom of the bottle cap plate, and the inner wall of the bottle cap ring is slidably connected to the first sealing base and the outer wall of the sealing ring;

[0023] A sealing rubber ring is located inside the bottle cap ring, and the top is fixedly connected to the bottom of the bottle cap plate, and the outer wall of the sealing rubber ring is slidably connected to the inner wall of the sealing ring;

[0024] A pressing component, one end of which is connected to the bottle cover plate;

[0025] The lock component has one end connected to the pressing component and the other end connected to the outer wall of the bottle cap ring.

[0026] Furthermore, the pressing component includes:

[0027] A connecting column, one end of which is fixedly connected to the top surface of the bottle cover plate;

[0028] A pressing plate is sleeved on the connecting column, and a receiving groove is provided on the top surface of the pressing plate, and a connecting hole is provided on the bottom surface of the receiving groove to penetrate the pressing plate;

[0029] A spring is sleeved on the connecting column, and one end of the spring is fixedly connected to the bottom surface of the pressing plate, and the other end of the spring is fixedly connected to the top surface of the bottle cover plate;

[0030] A limiting ring is sleeved on one end of the connecting column extending out of the pressing plate;

[0031] A pressing block plate, one end of which is fixedly connected to the bottom of the pressing plate;

[0032] The outer wall of the locking block is fixedly connected to the pressing plate, and the locking block is clamped with the locking assembly.

[0033] Furthermore, the lock assembly comprises:

[0034] A first rotating base, an outer wall of which is fixedly connected to an outer wall of the bottle cap ring, and the first rotating base is provided with a first rotating hole;

[0035] A first rotating shaft is inserted into the first rotating hole and is rotatably connected to the first rotating base;

[0036] The lock piece is sleeved on the first rotating shaft, and has a second rotating hole at one end and is clamped with the locking block at the other end.

[0037] Furthermore, the sealing buckle assembly includes:

[0038] A second rotating base, an outer wall of which is fixedly connected to an outer wall of the bottle cap ring, and a third rotating hole is formed in the second rotating base;

[0039] The second rotating shaft is inserted into the third rotating hole and is rotatably connected to the second rotating base;

[0040] The sealing buckle is sleeved on the second rotating shaft, and one end of the sealing buckle is provided with a fourth rotating hole, and the other end is clamped with the sealing rubber ring.

[0041] The above solution of the utility model includes at least the following beneficial effects:

[0042] The utility model provides a cell culture bottle for culturing umbilical cord stem cells. The bottle mouth component can be sealed by the cooperation between a pressing lock component and a sealing buckle component, thereby improving the experiment success rate of the user.

[0043] The push lock assembly and the sealing buckle assembly can be released from the bottle mouth assembly by pressing the push lock assembly, thereby preventing the push lock assembly and the sealing buckle assembly from being unable to be detached from the bottle mouth assembly when the internal and external air pressure difference between the bottle and the bottle is too large, thereby facilitating use by users. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is a three-dimensional schematic diagram of a cell culture bottle for culturing umbilical cord stem cells of the utility model;

[0045] Figure 2 It is an exploded schematic diagram of a cell culture bottle for culturing umbilical cord stem cells of the utility model;

[0046] Figure 3 This is a schematic diagram of an explosion of a bottle mouth assembly of a cell culture bottle for culturing umbilical cord stem cells according to the utility model;

[0047] Figure 4 It is an exploded schematic diagram of the sealing buckle assembly of the cell culture bottle for umbilical cord stem cell culture of the utility model;

[0048] Figure 5 This is a schematic diagram of an explosion of a push lock assembly of a cell culture bottle for umbilical cord stem cell culture of the utility model;

[0049] Figure 6The utility model is a schematic cross-sectional view of a push lock assembly of a cell culture bottle for culturing umbilical cord stem cells.

[0050] Description of reference numerals:

[0051] 1. Bottle body; 11. Liquid inlet hole; 21. Connecting tube; 220. Square tube; 221. Sealing rubber ring; 222. First sealing base; 223. Sealing ring; 31. Bottle cover plate; 32. Bottle cover ring; 33. Sealing rubber ring; 341. Connecting column; 342. Pressing plate; 3421. Accommodating groove; 3422. Connecting hole; 343. Spring; 344. Limiting ring; 345. Pressing block plate; 346. Locking block; 351. First rotating base; 3511. First rotating hole; 352. First rotating shaft; 353. Locking piece; 3531. Second rotating hole; 41. Second rotating base; 411. Third rotating hole; 42. Second rotating shaft; 43. Sealing buckle; 431. Fourth rotating hole. DETAILED DESCRIPTION

[0052] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0053] like Figures 1 to 6 As shown, an embodiment of the utility model provides a cell culture bottle for culturing umbilical cord stem cells, comprising: a bottle body 1, wherein a liquid inlet hole 11 is provided on the bottle body 1;

[0054] A bottle mouth assembly, one end of which covers the liquid inlet hole 11 and is connected to the bottle body 1;

[0055] Press the lock assembly to connect the bottle mouth assembly at one end;

[0056] The sealing buckle assembly is arranged on the push lock assembly.

[0057] In the embodiment of the utility model, the bottle body 1 is a square bottle body 1; the staff covers the push lock assembly on the bottle mouth assembly and then presses it manually, thereby locking the push lock assembly on the bottle mouth assembly and sealing the bottle mouth assembly, and applying pressure to the sealing buckle assembly through the push lock assembly to fix the push lock assembly and the sealing buckle assembly on the bottle mouth assembly, and seal the bottle mouth assembly.

[0058] like Figures 1 to 6 As shown, the bottle mouth assembly includes:

[0059] A connecting tube 21, one end of which covers the liquid inlet hole 11;

[0060] One end of the square pipe is fixedly connected to the other end of the connecting pipe 21.

[0061] In the embodiment of the utility model, the connecting tube 21 is a step structure, one end of which is a large opening covering the liquid inlet hole 11, and the other end is a small opening connected to the push lock assembly; the square tube 220 is sealed and fixed to the bottle body 1 through the connecting tube 21, which is convenient for the user to perform experimental operations in the bottle body 1 through the square tube 220 and the connecting tube 21.

[0062] like Figures 1 to 6 As shown, the square tube includes:

[0063] A square tube 220, one end of which is fixedly connected to the other end of the connecting tube 21, and the other end of which is connected to the push lock assembly;

[0064] The sealing rubber ring 221 is sleeved on one end of the square tube 220 and fixedly connected to the outer wall of the square tube 220;

[0065] The first sealing base 222 is sleeved on the other end of the square tube 220, and the inner wall is fixedly connected to the outer wall of the square tube 220;

[0066] The bottom of the sealing ring 223 is fixedly connected to the top surface of the first sealing base 222 and the other end of the square tube 220 .

[0067] In the embodiment of the utility model, the sealing rubber ring 221 and the first sealing base 222 are both sleeved on the square tube 220, and a snap groove is left between the sealing rubber ring 221 and the first sealing base 222, so that the sealing snap assembly can seal and clamp the square tube 220 conveniently; the bottom of the sealing ring 223 is welded and fixed to the top surface of the first sealing base 222 and the mouth of the square tube 220, so that the pressing lock assembly can seal the square tube 220 conveniently.

[0068] like Figures 1 to 6 As shown, the push lock assembly includes:

[0069] The bottle cover plate 31 covers the other end of the square tube 220 and abuts against the sealing ring 223;

[0070] The top of the bottle cap ring 32 is fixedly connected to the bottom of the bottle cap plate 31, and the inner wall of the bottle cap ring 32 is slidably connected to the first sealing base 222 and the outer wall of the sealing ring 223;

[0071] The sealing rubber ring 33 is located inside the bottle cap ring 32, and the top is fixedly connected to the bottom of the bottle cap plate 31, and the outer wall of the sealing rubber ring 33 is slidably connected to the inner wall of the sealing ring 223;

[0072] A pressing component, one end of which is connected to the bottle cover plate 31;

[0073] The lock assembly has one end connected to the pressing assembly and the other end connected to the outer wall of the bottle cap ring 32 .

[0074] In the embodiment of the utility model, the bottle cap ring 32 and the sealing rubber ring 33 are both fixedly connected to the bottom of the bottle cap plate 31, and the space between the bottle cap ring 32 and the sealing rubber ring 33 is convenient for sealing the square tube 220, wherein the space between the bottle cap ring 32 and the sealing rubber ring 33 can just be sleeved on the sealing ring 223, and then the bottle cap plate 31 and the square tube 220 are sealed and connected together through the bottle cap ring 32, the sealing rubber ring 33 and the sealing ring 223, which can prevent the external environment from contaminating the culture in the bottle, and at the same time, through the cooperation between the pressing component and the locking component, the square tube 220 can be completely sealed using the bottle cap plate 31.

[0075] like Figures 1 to 6 As shown, the pressing component includes:

[0076] A connecting column 341, one end of which is fixedly connected to the top surface of the bottle cover plate 31;

[0077] The pressing plate 342 is sleeved on the connecting column 341, and a receiving groove 3421 is formed on the top surface of the pressing plate 342, and a connecting hole 3422 is formed on the bottom surface of the receiving groove 3421 and penetrates the pressing plate 342;

[0078] The spring 343 is sleeved on the connecting column 341, and one end of the spring 343 is fixedly connected to the bottom surface of the pressing plate 342, and the other end of the spring 343 is fixedly connected to the top surface of the bottle cover plate 31;

[0079] A limiting ring 344 is sleeved on one end of the connecting column 341 extending out of the pressing plate 342;

[0080] A pressing block plate 345, one end of which is fixedly connected to the bottom of the pressing plate 342;

[0081] The outer wall of the locking block 346 is fixedly connected to the pressing plate 342, and the locking block 346 is engaged with the locking assembly.

[0082] In the embodiment of the utility model, the locking block 346 is a special-shaped structure, one end of which is a wavy structure, which is convenient for the lock assembly to be clamped; during installation, one end of the connecting column 341 is first fixed to the center position of the top surface of the bottle cover plate 31, and then the spring 343 is sleeved on the connecting column 341, and one end is fixedly connected to the top surface of the bottle cover plate 31, and then the pressing plate 342 is sleeved on the connecting column 341 through the connecting hole 3422, and at the same time, the other end of the spring 343 is fixedly connected to the bottom surface of the pressing plate 342, and then the pressing plate 342 is sleeved on the connecting column 341. The pressure block plate 345 is welded to the left and right sides of the pressing plate 342, and the limiting ring 344 is welded and fixed to one end of the connecting column 341 that passes through the pressing plate 342, so that the limiting ring 344 can be inserted into the accommodating groove 3421 under the action of the spring 343, and then the locking block 346 is welded to the front and back sides of the limiting ring 344, so that when the user presses the pressing plate 342, the locking block 346 is clamped with the lock assembly, so that the bottle cover plate 31 applies a certain pressure to the square tube 220 to facilitate sealing.

[0083] like Figures 1 to 6 As shown, the lock assembly includes:

[0084] A first rotating base 351, an outer wall of which is fixedly connected to the outer wall of the bottle cap ring 32, and a first rotating hole 3511 is formed in the first rotating base 351;

[0085] The first rotating shaft 352 is inserted into the first rotating hole 3511 and is rotatably connected to the first rotating base 351;

[0086] The locking member 353 is sleeved on the first rotating shaft 352 , and has a second rotating hole 3531 formed at one end and is engaged with the locking block 346 at the other end.

[0087] In the embodiment of the utility model, the locking piece 353 is an L-shaped structure, and the other end is wavy, which can just fit with the wavy structure of the locking block 346; when the pressing plate 342 is pressed down, it drives the locking block 346 to move downward, so that after the locking block 346 drops to a certain height, it can just fit with the locking piece 353. At this time, the locking piece 353 rotates with the first rotating shaft 352 as the rotating shaft, so that the locking piece 353 and the locking block 346 are just clamped together, so that the pressing plate 342 applies a constant pressure to the bottle cover plate 31 to ensure the sealing of the bottle cover plate 31 and the square tube 220.

[0088] like Figures 1 to 6 As shown, the sealing buckle assembly includes:

[0089] The second rotating base 41 has an outer wall fixedly connected to the outer wall of the bottle cap ring 32, and the second rotating base 41 is provided with a third rotating hole 411;

[0090] The second rotating shaft 42 is inserted into the third rotating hole 411 and is rotatably connected to the second rotating base 41;

[0091] The sealing fastener 43 is sleeved on the second rotating shaft 42 , and a fourth rotating hole 431 is formed at one end of the sealing fastener 43 , and the other end is engaged with the sealing rubber ring 221 .

[0092] In the embodiment of the utility model, the sealing clip 43 is an E-shaped structure, which is convenient for docking with the clip groove left between the sealing rubber ring 221 and the first sealing base 222, and for clipping the sealing rubber ring 221; in use, when the pressing plate 342 is pressed down, it drives the pressing block plate 345 to apply pressure to the sealing clip 43 through the inclined surface of the sealing clip 43, so that the sealing clip 43 is just docked with the clip groove left between the sealing rubber ring 221 and the first sealing base 222, and is sealed and clipped through the sealing rubber ring 221, which further facilitates the sealing of the square tube 220.

[0093] In a specific implementation manner: first, the staff seals the installed bottle cover plate 31 with the square tube 220 along the sealing ring 223, and then presses the pressing plate 342, so that the pressing plate 342 moves along the connecting column 341 toward the bottle cover plate 31. When the pressing plate 342 drives the locking block 346 to move downward, so that the locking block 346 drops to a height that can just fit with the locking member 353, the locking member 353 is clamped on the locking block 346, and then the bottle cover plate 31 is pressure-sealed against the square tube 220. At the same time, the pressing plate 342 moves and also drives the pressing member 353 to move downward. The pressing plate 345 moves downward, and when the locking piece 353 is engaged with the locking block 346, the pressing plate 345 just engages the sealing fastener 43 with the locking groove left between the sealing rubber ring 221 and the first sealing base 222, and seals and engages with the sealing rubber ring 221, further fixing the bottle cover plate 31 on the square tube 220, so that the sealing of the two is optimal, and at the same time, it can also prevent the locking piece 353 and the locking block 346 from being released by pressing the pressing plate 342 when the pressure difference between the inside and outside of the bottle is too large, thereby facilitating the user to open the bottle.

[0094] The above is a preferred embodiment of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A cell culture bottle for culturing umbilical cord stem cells, characterized in that: include: A bottle body (1), wherein a liquid inlet hole (11) is provided on the bottle body (1); A bottle mouth assembly, one end of which covers the liquid inlet hole (11) and is connected to the bottle body (1); Press the lock assembly to connect the bottle mouth assembly at one end; The sealing buckle assembly is arranged on the push lock assembly.

2. A cell culture bottle for culturing umbilical cord stem cells according to claim 1, characterized in that: The bottle mouth assembly comprises: A connecting tube (21), one end of which covers the liquid inlet hole (11); A square pipe fitting has one end fixedly connected to the other end of the connecting pipe (21).

3. A cell culture bottle for culturing umbilical cord stem cells according to claim 2, characterized in that: The square pipe fitting comprises: A square tube (220), one end of which is fixedly connected to the other end of the connecting tube (21), and the other end of which is connected to the push lock assembly; A sealing rubber ring (221) is sleeved on one end of the square tube (220) and fixedly connected to the outer wall of the square tube (220); A first sealing base (222) is sleeved on the other end of the square tube (220), and the inner wall is fixedly connected to the outer wall of the square tube (220); The bottom of the sealing ring (223) is fixedly connected to the top surface of the first sealing base (222) and the other end of the square tube (220).

4. A cell culture bottle for culturing umbilical cord stem cells according to claim 3, characterized in that: The push lock assembly comprises: A bottle cover plate (31) covers the other end of the square tube (220) and abuts against the sealing ring (223); A bottle cap ring (32), the top of which is fixedly connected to the bottom of the bottle cap plate (31), and the inner wall of the bottle cap ring (32) is slidably connected to the first sealing base (222) and the outer wall of the sealing ring (223); A sealing rubber ring (33) is located inside the bottle cover ring (32), and the top is fixedly connected to the bottom of the bottle cover plate (31), and the outer wall of the sealing rubber ring (33) is slidably connected to the inner wall of the sealing ring (223); A pressing assembly, one end of which is connected to the bottle cover plate (31); The lock assembly has one end connected to the pressing assembly and the other end connected to the outer wall of the bottle cap ring (32).

5. A cell culture bottle for culturing umbilical cord stem cells according to claim 4, characterized in that: The pressing component comprises: A connecting column (341), one end of which is fixedly connected to the top surface of the bottle cover plate (31); A pressing plate (342) is sleeved on the connecting column (341), and a receiving groove (3421) is provided on the top surface of the pressing plate (342), and a connecting hole (3422) is provided on the bottom surface of the receiving groove (3421) to penetrate the pressing plate (342); A spring (343) is sleeved on the connecting column (341), and one end of the spring is fixedly connected to the bottom surface of the pressing plate (342), and the other end of the spring is fixedly connected to the top surface of the bottle cover plate (31); A limiting ring (344) is sleeved on one end of the connecting column (341) extending out of the pressing plate (342); A pressing block plate (345), one end of which is fixedly connected to the bottom of the pressing plate (342); The outer wall of the locking block (346) is fixedly connected to the pressing plate (342), and the locking block (346) is clamped with the locking assembly.

6. A cell culture bottle for culturing umbilical cord stem cells according to claim 5, characterized in that: The lock assembly comprises: A first rotating base (351), the outer wall of which is fixedly connected to the outer wall of the bottle cap ring (32), and the first rotating base (351) is provided with a first rotating hole (3511); A first rotating shaft (352) is inserted into the first rotating hole (3511) and is rotatably connected to the first rotating base (351); The locking member (353) is sleeved on the first rotating shaft (352), and has a second rotating hole (3531) at one end and is engaged with the locking block (346) at the other end.

7. A cell culture bottle for culturing umbilical cord stem cells according to claim 6, characterized in that: The sealing buckle assembly comprises: A second rotating base (41), the outer wall of which is fixedly connected to the outer wall of the bottle cap ring (32), and the second rotating base (41) is provided with a third rotating hole (411); The second rotating shaft (42) is inserted into the third rotating hole (411) and is rotatably connected to the second rotating base (41); The sealing fastener (43) is sleeved on the second rotating shaft (42), and a fourth rotating hole (431) is opened at one end of the sealing fastener (43), and the other end is clamped with the sealing rubber ring (221).

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