Efficient sealed cell cryopreservation tube
By introducing an inflatable sealing device and locking structure into the cell frozen storage tube, the problem of insufficient sealing of the frozen storage tube in the prior art is solved, and more efficient sealing effect and convenient operation are achieved.
Patent Information
- Application Number
- CN202421005511.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-10
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2034-05-10
AI Technical Summary
The cell frozen storing tubes in the prior art have insufficient sealing properties, resulting in liquid nitrogen or water that may flow into the frozen storing tube and contaminate the sample.
An efficiently sealed cell freezing tube is designed, and an inflatable sealing device is adopted, including a sealing plug, a built-in shell, a sealing airbag and an inflatable assembly. The expansion and stable locking of the sealing airbag are achieved through the piston rod and locking structure, further enhancing the sealing ability.
It effectively improves the sealing of the frozen storage tube, reduces air leakage, ensures the cleanliness of the sample, and facilitates the disassembly and assembly of the frozen storage tube.
Smart Images
Figure CN222425187U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field related to cell cryopreservation tubes, in particular to a highly efficient sealed cell cryopreservation tube. Background Art
[0002] Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in liquid nitrogen at -196℃ can temporarily take the cells out of the growth state and preserve their cell characteristics. In this way, the cells can be revived for experiments when needed. In addition, moderately preserving a certain amount of cells can prevent cell loss due to contamination of the cells being cultured or other unexpected events, thus playing a role in cell preservation.
[0003] Cryotubes are a type of cryopreservation container commonly used in cell culture. Due to the large temperature difference during the freezing and thawing process, higher requirements are placed on cryotubes.
[0004] However, it is difficult to ensure the sealing between the tube cover and the tube body of the cell cryopreservation tube in the prior art. Sometimes, liquid nitrogen or water may flow into the cell cryopreservation tube from between the cover and the tube body, which may easily contaminate the sample in the cell cryopreservation tube. Utility Model Content
[0005] Therefore, in order to solve the above-mentioned shortcomings, the utility model provides a highly efficient sealed cell cryopreservation tube.
[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a highly efficient and sealed cell cryopreservation tube, comprising a cryopreservation tube body, a base being integrally connected to the bottom of the cryopreservation tube body, a connecting thread being provided on the outer side of the upper end of the cryopreservation tube body, and the cryopreservation tube body being threadedly connected to a sealing cover through the connecting thread provided on the outer side of the upper end, and also comprising an inflation sealing device arranged inside the sealing cover, the inflation sealing device comprising a sealing plug arranged inside the sealing cover, an internal shell internally arranged inside the sealing plug, a sealing airbag installed on the outer side of the sealing plug and abutting against the inner wall of the cryopreservation tube body, and an inflation component installed inside the internal shell and connected to the sealing airbag.
[0007] Preferably, the inflation assembly includes an inflation cylinder arranged inside the built-in shell, an air pipe installed on the outside of the inflation cylinder, a piston built inside the inflation cylinder, a piston rod inserted into the middle of the piston and vertically arranged upward, a connecting pipe installed at the upper end of the inflation cylinder, a locking structure built inside the piston rod, and an unlocking structure arranged relatively to the upper end of the locking structure.
[0008] Preferably, the locking structure includes a pin fastened to the inside of the piston rod, a spring sheet clamped to the outside of the pin, a lock shaft arranged at the lower end of the spring sheet and plugged into the side of the piston rod, and a lock groove opened in the connecting tube and connected to the outer end of the lock shaft.
[0009] Preferably, the unlocking structure includes a push shaft inserted into the inside of the piston rod, a pressure rod arranged at the bottom of the push shaft, a guide rod inserted into the bottom of the push shaft, a compression spring installed on the outside of the guide rod, a support block fastened to the lower end of the guide rod and fixedly connected to the inside of the piston rod, and a limiting ring arranged inside the piston rod.
[0010] Preferably, the air pipes are installed correspondingly along the four corners of the outer side of the inflation cylinder, and the air pipes on the four sides are connected to the sealing airbags.
[0011] Preferably, the interior of the connecting tube is vertically plugged into and assembled with the piston rod, and the upper end of the connecting tube is tightly connected to the built-in shell.
[0012] Preferably, the spring sheet is arranged in an inverted V shape as a whole, and locking shafts are fastened with bolts on both sides of the bottom of the spring sheet.
[0013] Preferably, the pressure rods are symmetrically arranged along the bottom of the push shaft, and the lower ends of the pressure rods on both sides are inclined and bent.
[0014] Beneficial effects of the utility model:
[0015] Advantage 1: The utility model provides an inflatable sealing device, which can enhance the sealing of the cryopreservation tube with the initial cooperation of the sealing plug. The sealing airbag and the inflatable component can be used to expand and seal the cryopreservation tube, thereby further enhancing the sealing of the cryopreservation tube.
[0016] Advantage 2: The utility model is provided with a locking structure, that is, when the piston rod is squeezed and inflated, the locking shafts provided on both sides of the bottom of the spring sheet can be moved to the positions of the locking grooves provided on both sides, so that the locking shafts are quickly plugged and locked with the locking grooves under the elastic force, thereby ensuring the stability of the sealing state of the sealing airbag expansion and reducing air leakage.
[0017] Advantage 3: The utility model is provided with an unlocking structure, that is, the spring sheet can be compressed under the pressure of the pressure rod provided at the bottom of the push shaft, and the lock shaft and the lock groove can be quickly unlocked, thereby releasing the expansion state of the sealing airbag, so that the cryotube body and the sealing cover can be conveniently disassembled and assembled. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the structure of the utility model;
[0019] Figure 2 It is a schematic diagram of the split structure of the utility model;
[0020] Figure 3 This is a schematic diagram of the internal structure of the utility model;
[0021] Figure 4 This is a schematic diagram of the front view structure of the inflatable sealing device of the utility model;
[0022] Figure 5 This is a schematic diagram of the internal structure of the inflatable component of the utility model from the front view;
[0023] Figure 6 This is a front view structural diagram of the locking structure of the utility model;
[0024] Figure 7 It is a front view structural schematic diagram of the unlocking structure of the utility model.
[0025] Among them: the cryogenic tube body -1, the base -2, the connecting thread -3, the sealing cover -4, the inflation sealing device -5, the sealing plug -51, the built-in shell -52, the sealing airbag -53, the inflation component -54, the inflation cylinder -541, the air pipe -542, the piston -543, the piston rod -544, the connecting tube -545, the locking structure -5a, the pin shaft -5a1, the spring sheet -5a2, the locking shaft -5a3, the locking groove -5a4, the unlocking structure -5b, the push shaft -5b1, the pressure rod -5b2, the guide rod -5b3, the compression spring -5b4, the support block -5b5, and the limiting ring -5b6. DETAILED DESCRIPTION
[0026] In order to further explain the technical solution of the present utility model, it is described in detail through specific embodiments below.
[0027] See also Figure 1-3 The utility model provides a highly efficient sealed cell cryopreservation tube, including a cryopreservation tube body 1, a base 2 is integrally connected to the bottom of the cryopreservation tube body 1, a connecting thread 3 is provided on the outer side of the upper end of the cryopreservation tube body 1, and the cryopreservation tube body 1 is threadedly connected with a sealing cover 4 through the connecting thread 3 provided on the outer side of the upper end, and also includes an inflation sealing device 5 provided inside the sealing cover 4.
[0028] See also Figure 4 The inflation sealing device 5 in this embodiment includes a sealing plug 51 made of silicone material and built into the sealing cover 4, a built-in shell 52 corresponding to the sealing plug 51 to realize the assembly and installation of other parts, and a sealing airbag 53 corresponding to the outer side of the sealing plug 51 and abutting against the inner wall of the upper end of the freezing tube body 1. The overall material of the sealing airbag 53 is consistent with that of the sealing plug 51, both of which are made of silicone material, and an inflation component 54 correspondingly installed in the built-in shell 52 and connected to the sealing airbag 53, so that efficient inflation and sealing can be achieved.
[0029] See also Figure 5-7The inflation component 54 in this embodiment includes an inflation cylinder 541 which is fastened to the lower end of the inner part of the built-in shell 52, an air pipe 542 which is correspondingly plugged and connected to the outer side of the inflation cylinder 541 to realize air transmission or suction cooperation, a piston 543 which is correspondingly built into the inflation cylinder 541 to realize extrusion inflation or pulling and pumping, a piston rod 544 which is vertically plugged into the middle of the piston 543 and vertically arranged upward, and the top of the piston rod 544 extends to the middle of the upper end of the sealing cover 4, a connecting pipe 545 which is vertically fastened to the upper end of the inflation cylinder 541 and connected to the outer side of the piston rod 544, a locking structure 5a which is correspondingly built into the piston rod 544 to realize inflation locking cooperation, and an unlocking structure 5b which is correspondingly arranged at the upper end of the locking structure 5a to realize convenient unlocking and pumping cooperation.
[0030] Among them, the locking structure 5a includes a pin shaft 5a1 which is longitudinally fastened to the inside of the piston rod 544 for connection, a spring sheet 5a2 which is correspondingly clamped on the outside of the pin shaft 5a1 for elastic transmission, and a lock shaft 5a3 which is correspondingly installed on the lower ends of both sides of the spring sheet 5a2 and plugged into the left and right sides of the piston rod 544. The outer part of the lock shaft 5a3 is polished and smooth without barbs, and the outer plug-in end of the lock shaft 5a3 is round-headed, and lock grooves 5a4 are symmetrically opened on the left and right sides of the connecting tube 545 and plugged into and locked with the lock shafts 5a3 on both sides. The lock grooves 5a4 on both sides are opened in the shape of triangular grooves, which ensure the stability of plug-in locking and the rapidity of subsequent unlocking efficiency.
[0031] Among them, the unlocking structure 5b includes a push shaft 5b1 vertically inserted into the piston rod 544 and extending to the outside of the upper end of the piston rod 544, a pressure rod 5b2 symmetrically installed at the bottom of the push shaft 5b1 to realize the push and compression fit of the spring sheet 5a2, a guide rod 5b3 correspondingly vertically inserted into the bottom of the push shaft 5b1, a compression spring 5b4 installed on the outside of the guide rod 5b3 and in contact with the bottom of the push shaft 5b1, so that the rebound force of the compression spring 5b4 can be cooperated to realize automatic reset fit, a support block 5b5 correspondingly fastened to the lower end of the guide rod 5b3 and fixedly connected to the inside of the piston rod 544, and a limit ring 5b6 correspondingly installed in the piston rod 544 and in contact with the bottom of the push shaft 5b1, so that the reset limit fit of the push shaft 5b1 can be realized to avoid the occurrence of the phenomenon of excessive moving distance.
[0032] Among them, the air pipe 542 is installed correspondingly along the four corners of the outer side of the inflation cylinder 541, and the air pipes 542 on the four sides are connected to the sealing airbag 53, ensuring that the four sides of the sealing airbag 53 can be inflated or evacuated synchronously, ensuring the rapidity of the inflation and sealing activities; the interior of the connecting tube 545 is vertically plugged and combined with the piston rod 544, and the upper end of the connecting tube 545 is tightly connected to the built-in shell 52, ensuring that the connecting tube 545 can assist in realizing the guiding cooperation of the piston rod 544 moving up and down; the spring sheet 5a2 is arranged in an inverted V shape as a whole, and the locking shaft 5a3 is bolted on both sides of the bottom of the spring sheet 5a2, so that the locking shaft 5a3 installed on both sides of the lower end of the spring sheet 5a2 can realize efficient plug-in locking cooperation; the pressure rod 5b2 is symmetrically arranged along the bottom of the push shaft 5b1, and the lower ends of the pressure rods 5b2 on both sides are inclined and bent, ensuring that the pressure rods 5b2 on both sides can squeeze the spring sheet 5a2 correspondingly to realize convenient unlocking cooperation.
[0033] Here’s how it works:
[0034] When the cell cryopreservation tube is to be used, the sealing cap 4 threadedly connected to the upper end of the cryopreservation tube body 1 can be rotated to open the upper end opening of the cryopreservation tube body 1. In this way, the cells to be stored can be reinforced and placed inside the cryopreservation tube body 1, and the cryopreservation tube body 1 and the sealing cap 4 can be threadedly connected again to achieve auxiliary sealing cooperation.
[0035] In order to help improve the overall sealing of the cryopreservation tube body 1 and prevent water vapor from entering the cryopreservation tube body 1 and affecting the stored cells, an inflatable sealing device 5 is provided inside the sealing cover 4. First, a sealing plug 51 built into the sealing cover 4 can be plugged into the upper end of the cryopreservation tube body 1 when the sealing cover 4 is connected to the cryopreservation tube body 1, so as to achieve the sealing and matching of the upper end opening of the cryopreservation tube body 1 and improve the overall sealing of the cryopreservation tube body 1.
[0036] Secondly, in order to further improve the sealing effect of the cryopreservation tube body 1, the piston rod 544 provided inside the inflation component 54 can be squeezed to enable the piston rod 544 to push down the piston 543 connected to the bottom. In this way, the piston 543 can move downward along the inflation cylinder 541 to push out the air inside the inflation cylinder 541, and cooperate with the air pipe 542 provided at the four corners of the outer end of the inflation cylinder 541 to inflate the sealing air bag 53 provided outside the sealing plug 51, so that the sealing air bag 53 expands as a whole and fits tightly against the inner wall of the upper end of the cryopreservation tube body 1, thereby blocking the gap between the cryopreservation tube body 1 and the sealing plug 51, and realizing the enhancement of the overall sealing performance. During the extrusion process of the piston rod 544, the locking structure 5a arranged inside the piston rod 544 will move downward synchronously, that is, the locking shafts 5a3 installed on both sides of the lower end of the spring sheet 5a2 will move to the relative position with the locking grooves 5a4 opened on both sides of the connecting tube 545. The spring sheet 5a2 in the compressed state will rebound to push out the locking shafts 5a3 on both sides, so that the locking shafts 5a3 on both sides are inserted into the corresponding locking grooves 5a4, thereby realizing the auxiliary locking cooperation, so that the sealing airbag 53 in the inflated and expanded sealing state can maintain a stable state, reduce the occurrence of air leakage, and achieve the advantages of auxiliary inflation sealing and flexible locking cooperation;
[0037] If the expansion sealing state of the sealing airbag 53 needs to be released later, the push shaft 5b1 plugged into the upper end of the piston rod 544 can be squeezed to make the push shaft 5b1 squeeze the pressure rods 5b2 provided on both sides of the bottom, so that the pressure rods 5b2 on both sides cooperate with the inclined bent ends of the bottom to contact the left and right sides of the spring sheet 5a2, so that the spring sheet 5a2 is squeezed by the pressure rods 5b2 on both sides to achieve compression activity. In this way, the lock shafts 5a3 provided on the left and right sides of the lower end of the spring sheet 5a2 will smoothly move into the piston rod 544 to release the airbag 53. The locking grooves 5a4 on both sides of the connecting tube 545 are used to realize convenient locking activities, thereby the piston rod 544 can be pulled up as a whole, so that the piston 543 cooperates with the inflator 541 to realize the exhaust cooperation, so that the air inside the sealing airbag 53 will be collected into the inflator 541 along the air pipe 542, and the sealing airbag 53 will be deflated, releasing the expansion-resisting sealing effect, so that the rear sealing cover 4 can be quickly and conveniently disassembled and assembled with the cryopreservation tube body 1, thereby achieving the advantage of conveniently releasing the expansion sealing state of the sealing airbag 53;
[0038] Among them, during the extrusion process of the push shaft 5b1, the guide rod 5b3 inserted in the middle of the lower end will cooperate to realize the stable downward extrusion activity, and during the extrusion, the compression spring 5b4 arranged on the outside of the guide rod 5b3 will be squeezed, so that when the unlocking activity is completed later, the compressed compression spring 5b4 can cooperate with the rebound force to realize the rotation and upward reset of the push shaft 5b1, and cooperate with the limit ring 5b6 to realize the reset limit cooperation, so as to avoid the occurrence of reset distance offset.
[0039] The utility model provides a highly sealed cell cryopreservation tube, which is provided with an inflatable sealing device 5, that is, under the preliminary cooperation of the sealing plug 51, the sealing performance of the cryopreservation tube can be enhanced, and the sealing airbag 53 and the inflatable component 54 can be cooperated to make the sealing airbag 53 expand and seal, thereby further enhancing the sealing performance of the cryopreservation tube, and a locking structure 5a is provided, that is, when the piston rod 544 is squeezed and inflated, the locking shafts 5a3 provided on both sides of the bottom of the spring sheet 5a2 can be moved to the positions of the locking grooves 5a4 provided on both sides, so that the locking shafts 5a3 are subjected to elastic force and are quickly plugged and locked with the locking grooves 5a4, thereby ensuring the stability of the expansion and sealing state of the sealing airbag 53 and reducing air leakage, and an unlocking structure 5b is provided, that is, under the squeezing of the pressure rod 5b2 provided at the bottom of the push shaft 5b1, the spring sheet 5a2 can be compressed, and the locking shaft 5a3 and the locking groove 5a4 can be quickly unlocked, thereby releasing the expansion state of the sealing airbag 53, so that the cryopreservation tube body 1 and the sealing cover 4 can be conveniently disassembled and assembled.
[0040] The above description is only a preferred example of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A highly efficient sealed cell cryopreservation tube, comprising a cryopreservation tube body (1), wherein the bottom of the cryopreservation tube body (1) is integrally connected with a base (2), a connecting thread (3) is provided on the outer side of the upper end of the cryopreservation tube body (1), and the cryopreservation tube body (1) is threadedly connected to a sealing cover (4) through the connecting thread (3) provided on the outer side of the upper end; Features: The invention also comprises an inflation sealing device (5) arranged inside the sealing cover (4), wherein the inflation sealing device (5) comprises a sealing plug (51) arranged inside the sealing cover (4), a built-in shell (52) built inside the sealing plug (51), a sealing air bag (53) installed outside the sealing plug (51) and abutting against the inner wall of the cryopreservation tube body (1), and an inflation component (54) installed inside the built-in shell (52) and connected to the sealing air bag (53).
2. A highly efficient sealed cell cryopreservation tube according to claim 1, characterized in that: The inflation assembly (54) comprises an inflation cylinder (541) disposed inside the internal shell (52), an air pipe (542) installed outside the inflation cylinder (541), a piston (543) embedded inside the inflation cylinder (541), a piston rod (544) inserted into the middle of the piston (543) and vertically disposed upward, a connecting pipe (545) installed at the upper end of the inflation cylinder (541), a locking structure (5a) embedded inside the piston rod (544), and an unlocking structure (5b) disposed relatively to the upper end of the locking structure (5a).
3. A highly efficient sealed cell cryopreservation tube according to claim 2, characterized in that: The locking structure (5a) comprises a pin shaft (5a1) fastened to the inside of the piston rod (544), a spring sheet (5a2) clamped to the outside of the pin shaft (5a1), a lock shaft (5a3) arranged at the lower end of the spring sheet (5a2) and plugged into the side of the piston rod (544), and a lock groove (5a4) opened in the connecting tube (545) and connected to the outer end of the lock shaft (5a3).
4. A highly efficient sealed cell cryopreservation tube according to claim 2, characterized in that: The unlocking structure (5b) comprises a push shaft (5b1) inserted into the piston rod (544), a pressure rod (5b2) arranged at the bottom of the push shaft (5b1), a guide rod (5b3) inserted into the bottom of the push shaft (5b1), a compression spring (5b4) installed on the outside of the guide rod (5b3), a support block (5b5) fastened to the lower end of the guide rod (5b3) and fixedly connected to the inside of the piston rod (544), and a limit ring (5b6) arranged inside the piston rod (544).
5. The highly efficient sealed cell cryopreservation tube according to claim 2, characterized in that: The air pipes (542) are installed correspondingly along the four corners of the outer side of the inflation tube (541), and the air pipes (542) on the four sides are all connected to the sealing airbags (53).
6. The highly efficient sealed cell cryopreservation tube according to claim 2, characterized in that: The interior of the connecting tube (545) is vertically plugged and assembled with the piston rod (544), and the upper end of the connecting tube (545) is tightly connected to the built-in shell (52).
7. The highly efficient sealed cell cryopreservation tube according to claim 3, characterized in that: The spring sheet (5a2) is arranged in an inverted V shape as a whole, and locking shafts (5a3) are fastened with bolts on both sides of the bottom of the spring sheet (5a2).
8. The highly efficient sealed cell cryopreservation tube according to claim 4, characterized in that: The pressure rods (5b2) are symmetrically arranged along the bottom of the push shaft (5b1) on the left and right, and the lower ends of the pressure rods (5b2) on both sides are inclined and bent.