Sample adding device

Abstract

The utility model discloses a sample adding device and relates to the technical field of embryo cultivation. Comprising a sample adding table, and the sample adding table is provided with two sets of semicircular limiting baffles, a lifting mechanical arm adjacent to the two sets of semicircular limiting baffles and a pushing and pulling-back mechanism; a clamping mechanism located right above the adjacent semicircular limiting baffles is arranged at the driving end of the lifting mechanical arm, and a culture solution sample adding pipe is clamped in the clamping mechanism; and a mineral oil injector is mounted on the pushing and pulling-back mechanism. According to the utility model, the situation that the subsequent embryo culture quality is influenced due to osmotic pressure rise caused by evaporation of liquid drops when a plurality of micro liquid drops are manually manufactured drop by drop and are exposed in the air for a long time can be avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of embryo cultivation, in particular to a sample adding device. Background Art

[0002] In the assisted reproductive embryo room, the droplet method is usually used to culture embryos, that is, each embryo is cultured in a small droplet (about 15μl of embryo culture solution) to facilitate observation and selection of embryos. In order to improve the efficiency of embryo operation and save space, multiple small droplets for embryo culture are usually made in a culture dish with a diameter of 35mm, so that multiple embryos can be cultured in one dish. At present, the droplets are prepared by manual method, that is, a Pasteur dropper is used to suck about 1ml of culture solution and spot it drop by drop on the bottom of the culture dish. After the droplet spotting is completed, it is necessary to quickly add about 3ml of mineral oil to cover it to prevent water evaporation.

[0003] The difficulty in preparing droplets using this manual method is that, in order to prevent the evaporation of water in the spotted droplets and thus affect the osmotic pressure of the culture medium, two people are required to work together: one to make the culture medium droplets and the other to add mineral oil. Since the droplets are manually applied drop by drop to the bottom of the culture dish, this is time-consuming and labor-intensive. When a small amount of droplets is exposed to the air for a long time, the evaporation of water in the droplets causes the osmotic pressure to increase, thus affecting the quality of subsequent embryo culture. Utility Model Content

[0004] In view of this, the utility model provides a sample adding device to solve the technical problems mentioned in the background technology.

[0005] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0006] A sample loading device comprises a sample loading platform, on which two groups of semicircular limit baffles are arranged, and a lifting mechanical arm and a push-pull-back mechanism are arranged adjacent to the two groups of semicircular limit baffles respectively;

[0007] The two groups of semicircular limit baffles on the sample loading platform are used to position the culture dish where the culture liquid droplets need to be made and the culture dish where the mineral oil is added.

[0008] The driving end of the lifting mechanical arm is provided with a clamping mechanism located just above the adjacent semicircular limit baffle;

[0009] The clamping mechanism is provided with a culture fluid adding tube, the top of the culture fluid adding tube is threadedly connected to the first cover body, the bottom of the culture fluid adding tube is provided with a plurality of conical liquid outlet gun heads, the inner cavity of the conical liquid outlet gun head is provided with a plug, and one group of the plugs protrudes from the liquid outlet of the conical liquid outlet gun head, a hollow push plate is provided inside the culture fluid adding tube, the lower end surface of the hollow push plate is provided with a plurality of connecting rods respectively connected to the plugs in the plurality of conical liquid outlet gun heads, the upper end surface of the hollow push plate is provided with a push rod, the top of the push rod is provided with a piston column, the top surface of the first cover body is provided with a piston sleeve that matches the gap of the piston column, a spring is provided between the top surface of the piston sleeve and the top of the piston column, and the spring makes the plug close to the liquid outlet of the conical liquid outlet gun head and blocks the liquid outlet of the conical liquid outlet gun head through its own elastic force, an inlet tube and an air pipe joint are provided on the side wall of the culture fluid adding tube, and the air pipe joint is connected to a high-pressure gas source equipment outside the device;

[0010] The push-pull mechanism is provided with a mineral oil syringe whose outlet is aligned with the center of the semicircular limit baffle adjacent to it, and the push-pull mechanism can pull up and push down the piston rod of the mineral oil syringe in the vertical direction.

[0011] Furthermore, infrared induction switches are embedded on the arc-shaped inner side walls of the two groups of semicircular limit baffles.

[0012] Furthermore, the clamping mechanism includes a sleeve with a notch on the side wall, the sleeve coincides with the axis of the semicircular limit baffle below it, a second cover body is threadedly connected to the top of the sleeve, the outer side of the sleeve is connected to the driving end of the lifting robot arm, the sleeve is covered on the outer periphery of the culture fluid sampling tube, the notch on the sleeve is used to expose the sampling tube and the air pipe joint of the culture fluid sampling tube, and an annular cover body is threadedly connected to the bottom end of the sleeve, as the annular cover body is screwed upward, the culture fluid sampling tube is supported and moved upward, so that the top surface of the first cover body on the culture fluid sampling tube abuts against the inner side surface of the second cover body at the top of the sleeve.

[0013] Furthermore, the lifting robot arm includes a square shell, which is vertically arranged on the sample loading table, and a vertical guide groove is opened on the main body of the shell. A first threaded screw is vertically mounted inside the shell through a bearing seat. A connecting block is threadedly connected to the first threaded screw. The connecting block is slidably connected to the vertical guide groove and protrudes horizontally from the outside of the square shell to form a driving end of the lifting robot arm. The square shell is also provided with a first driving motor that drives the first threaded screw inside the shell.

[0014] Furthermore, the push-pull-back mechanism includes a support column vertically arranged on the sample loading table, a fixing mechanism for locking the injection barrel of the mineral oil syringe is provided on the side of the support column body, and a vertical driving mechanism is provided at the top of the body, and the driving end of the vertical driving mechanism is detachably connected to the push cap at the top of the piston rod of the mineral oil syringe.

[0015] Furthermore, the fixing mechanism includes a positioning seat and a semicircular clamp. The positioning seat is installed on the side wall of the support column, and a V-shaped groove is opened on its main body. One end of the semicircular clamp is hinged to the positioning seat, and the other end is connected to the positioning seat by bolts to lock the mineral oil syringe barrel at the V-shaped groove on the positioning seat. The arc-shaped inner side of the semicircular clamp is provided with a rubber pad that contacts the outer wall of the mineral oil syringe barrel.

[0016] Furthermore, the vertical drive mechanism includes a bracket arranged at the top of the support column, and an axially vertical second threaded screw and a guide rod are arranged between the top of the bracket and the top surface of the support column. A connecting plate constituting a driving end of the vertical drive mechanism is threadedly connected to the body of the second threaded screw, and the guide rod vertically penetrates the connecting plate. A T-slot is provided on the connecting plate body into which a push cap at the top of the piston rod of a mineral oil syringe can be horizontally inserted. A second drive motor for driving the second threaded screw is also provided on the bracket.

[0017] The beneficial effects of the utility model are:

[0018] The lifting mechanical arm is used to drive the clamping mechanism to move downward, so that the culture solution adding tube moves downward. After it moves downward to a specified height, the plug at the bottom of the culture solution adding tube protruding from the outlet of the conical liquid outlet gun head contacts the inner bottom of the culture dish placed at the semicircular limit baffle just below it. Under the action of pressure, the plug overcomes the elastic force of the spring and pushes the hollow push plate upward through the connecting rod. At this time, other plugs also move upward in the conical liquid outlet gun head due to structural relationships. At this time, the outlet of the conical liquid outlet gun head at the bottom of the culture solution adding tube is all opened. At this time, the culture solution in the culture solution adding tube is at the high position connected to the trachea joint. Under the action of the high-pressure gas introduced by the compressed air source equipment, the liquid flows out from the liquid outlets of multiple conical liquid outlet gun tips, and quickly forms multiple small droplets for embryo culture at the bottom of the culture dish. After the droplets are made, the lifting robot arm drives the clamping mechanism to move up and return to its original position, and then quickly moves the culture dish to another set of semicircular limit baffles, so that the next step of mineral oil covering can be carried out, which can avoid the time-consuming manual production of multiple trace droplets drop by drop, and the long-term exposure to the air, which causes the evaporation of water in the droplets and causes the osmotic pressure to increase, thereby affecting the subsequent embryo culture quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The utility model is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0020] Figure 1 It is a structural schematic diagram of the utility model.

[0021] Figure 2 It is a half-section structural schematic diagram of a culture solution sample adding tube of the utility model being located in a sleeve.

[0022] Figure 3It is a structural schematic diagram of the lifting mechanical arm of the utility model.

[0023] 1. Sample loading platform, 2. Semicircular limit baffle, 3. Lifting mechanical arm, 300. Square shell, 301. Vertical guide groove, 302. First threaded screw, 303. Connecting block, 304. First driving motor, 4. Push-pull mechanism, 400. Support column, 401. Positioning seat, 402. Semicircular clamp, 403. Bracket, 404. Second threaded screw, 405. Guide rod, 406. Connecting plate, 407 , T-slot, 408, second drive motor, 5, culture solution sample tube, 6, first cover body, 7, conical liquid outlet gun head, 8, plug, 9, hollow push plate, 10, connecting rod, 11, push rod, 12, piston column, 13, piston sleeve, 14, spring, 15, sample injection tube, 16, air pipe joint, 17, mineral oil sample injection tube, 18, infrared sensor switch, 19, sleeve, 20, second cover body, 21, annular cover body. DETAILED DESCRIPTION

[0024] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] like Figure 1-3 As shown, the utility model provides a sample loading device, including a sample loading platform 1, on which are disposed two groups of semicircular limit baffles 2, and a lifting mechanical arm 3 and a push-pull-back mechanism 4 respectively adjacent to the two groups of semicircular limit baffles 2;

[0026] The two groups of semicircular limit baffles 2 on the sample loading platform 1 are used to position the culture dish for making culture liquid droplets and the culture dish for adding mineral oil, respectively. The culture dish is placed against the arc-shaped inner wall of the semicircular limit baffle 2, and the semicircular limit baffle 2 forms a position limit for the culture dish. Infrared sensor switches 18 are embedded on the arc-shaped inner walls of the two groups of semicircular limit baffles 2.

[0027] The driving end of the lifting mechanical arm 3 is provided with a clamping mechanism located just above the adjacent semicircular limit baffle 2;

[0028] A culture fluid sample tube 5 is clamped in the clamping mechanism, the top of the culture fluid sample tube 5 is threadedly connected to the first cover body 6, a plurality of conical liquid discharge gun tips 7 are arranged at the bottom of the culture fluid sample tube 5, a plug 8 is arranged in the inner cavity of the conical liquid discharge gun tip 7, and one group of plugs 8 protrudes from the liquid outlet of the conical liquid discharge gun tip 7, a hollow push plate 9 is arranged inside the culture fluid sample tube 5, a plurality of connecting rods 10 are arranged on the lower end surface of the hollow push plate 9, which are respectively connected to the plugs 8 in the plurality of conical liquid discharge gun tips 7, a push rod 11 is arranged on the upper end surface of the hollow push plate 9, a piston column 12 is arranged on the top of the push rod 11, and a piston column 12 is arranged inside the first cover body 6. The top surface is provided with a piston sleeve 13 which is in clearance with the piston column 12, and a spring 14 is provided between the inner top surface of the piston sleeve 13 and the top of the piston column 12. The spring 14 uses its own elastic force to make the plug 8 close to the liquid outlet of the conical liquid outlet gun head 7 and block the liquid outlet of the conical liquid outlet gun head 7. The side wall of the culture fluid sample addition tube 5 is provided with a sample injection tube 15 and an air pipe joint 16. The air pipe joint 16 is connected to a high-pressure gas source device outside the device. During a single use, if there is insufficient culture fluid, open the sealing cover of the tube mouth of the sample injection tube 15, and then add the sample through the sample injection tube 15;

[0029] A mineral oil injector 17 with its outlet aligned with the center of the adjacent semicircular limit baffle 2 is installed on the push-pull mechanism 4, and the push-pull mechanism 4 can pull up and push down the piston rod of the mineral oil injector 17 in the vertical direction.

[0030] When the infrared sensing switch 18 embedded in the inner side of the semicircular limit baffle 2 adjacent to the lifting robot arm 3 senses that a culture dish is placed there, the system host end carried by the sampling device can control the driving end of the lifting robot arm 3 to drive the culture fluid sampling tube 5 to rise or fall, and can also control the high-pressure gas source equipment outside the device connected to the air pipe joint 16 to open or close.

[0031] When the infrared sensor switch 18 embedded in the inner side of the semicircular limit baffle 2 adjacent to the push-pull mechanism 4 senses that a culture dish is placed there, the system host end of the sample adding device can control the push-pull mechanism 4 to pull up or push down the piston rod of the mineral oil syringe 17 in the vertical direction.

[0032] First, place the culture dish on the semicircular limit baffle 2 adjacent to the lifting mechanical arm 3. When the infrared sensor switch 18 embedded in the inner side of the group of semicircular limit baffles 2 senses that a culture dish is placed here, the system host end carried by the sampling device first controls the lifting mechanical arm 3, so that its driving end drives the culture solution sampling tube 5 to descend to the specified height. Then, the plug 8 at the bottom of the culture solution sampling tube 5 protruding from the outlet of the conical liquid outlet gun head 7 first contacts the inner bottom of the culture dish placed on the semicircular limit baffle 2 just below it, and the contact generates pressure. Under the action of pressure, the plug 8 overcomes the elastic force 14 of the spring and pushes up the hollow push plate 9 through the connecting rod 10. At this time, the other plugs 8 are also in the conical outlet due to structural relationships. The liquid gun head 7 is synchronously moved up, and the conical liquid outlet gun head 7 liquid outlets at the bottom of the culture fluid sample tube 5 are all opened at this time. The system host end carried by the sample adding device controls the high-pressure gas source equipment connected to the tracheal joint 16 to pass high-pressure gas into the culture fluid sample tube 5. Under the effect of the high-pressure gas passed, the culture fluid in the culture fluid sample tube 5 flows out rapidly from the liquid outlets of multiple conical liquid outlet gun heads 7 respectively, and can be quickly made into multiple small droplets for embryo culture at the bottom of the culture dish, so that the mineral oil covering work of the next step can be carried out rapidly, and the manual micro-droplets are avoided from being exposed to the air for a long time, so that the osmotic pressure is increased by evaporation of the droplet water, and then the situation that affects the quality of subsequent embryo culture occurs. After forming multiple small droplets for embryo culture in the culture dish, the system host end carried by the sample adding device controls the lifting mechanical arm 3, so that the driving end of the lifting mechanical arm 3 drives the culture fluid sample tube 5 to return to the origin, and at the same time, the system host end carried by the sample adding device also synchronously controls the high-pressure gas source equipment connected to the tracheal joint 16 to close, and stops the introduction of high-pressure gas.

[0033] When performing the mineral oil covering work, the culture dish with a plurality of small droplets for embryo culture formed on the bottom of the dish is moved to another set of semicircular limit baffles 2. When the infrared sensor switch 18 embedded in the inner side of the set of semicircular limit baffles 2 senses that a culture dish is placed there, the system host end carried by the sample adding device controls the push-pull mechanism 4 to push down the piston rod of the mineral oil syringe 17 in the vertical direction, so that the mineral oil in the mineral oil syringe 17 flows out from its outlet and falls into the culture dish below, so as to achieve the mineral oil covering work. When the outflow of mineral oil reaches the set value, the push-pull mechanism 4 stops pushing down the piston rod of the mineral oil syringe 17, and the outflow of mineral oil is controlled by controlling the downward movement distance of the plug rod component. When the mineral oil in the mineral oil syringe 17 is used up, the container filled with mineral oil can be placed at the outlet of the mineral oil syringe 17 so that the outlet of the mineral oil syringe 17 is located below the liquid surface, and then the system host end mounted on the sample adding device controls the push-pull mechanism 4 to pull up the piston rod of the mineral oil syringe 17 in the vertical direction, so that the mineral oil in the container is sucked into the mineral oil syringe 17, so as to achieve sample addition.

[0034] In this embodiment, the clamping mechanism includes a sleeve 19 with a notch on the side wall, the sleeve 19 coincides with the axis of the semicircular limit baffle 2 below it, the top of the sleeve 19 is threadedly connected to a second cover body 20, the outer side of the sleeve 19 is connected to the driving end of the lifting robot arm 3, the sleeve 19 is wrapped around the outer periphery of the culture fluid sampling tube 5, the sleeve 19 coincides with the axis of the semicircular limit baffle 2 below it, therefore, the culture fluid sampling tube 5 wrapped in the culture fluid sampling tube 5 is just above the semicircular limit baffle 2 adjacent to the lifting robot arm 3, the notch on the sleeve 19 is used to make the sample inlet 15 of the culture fluid sampling tube 5 and the gas injection tube 15 of the culture fluid sampling tube 5 The pipe joint 16 is exposed, and the bottom end of the sleeve 19 is threadedly connected to an annular cover body 21. As the annular cover body 21 is screwed upward, the culture fluid sampling tube 5 is supported and moved upward, so that the top surface of the first cover body 6 on the culture fluid sampling tube 5 abuts against the inner surface of the second cover body 20 at the top of the sleeve 19, so that the culture fluid sampling tube 5 remains stable in the sleeve 19 and does not shake. Because the service life of the culture fluid sampling tube 5 is limited and needs to be replaced with a new one regularly, there may be errors in the size of the consumable structure. Therefore, the structure of the annular cover body 21 is used for adjustment, which can adapt to structural errors and ensure that the culture fluid sampling tube 5 remains stable in the sleeve 19.

[0035] refer to Figure 3 In this embodiment, the lifting mechanical arm 3 includes a square housing 300, which is vertically arranged on the sample loading platform 1, and a vertical guide groove 301 is provided on the main body thereof, and a first threaded screw 302 is vertically arranged inside the housing through a bearing seat, and a connecting block 303 is threadedly connected to the first threaded screw 302, and the connecting block 303 is slidably connected to the vertical guide groove 301 and horizontally protrudes from the outside of the square housing 300 to form the driving end of the lifting mechanical arm, and a first driving motor 304 is also provided on the square housing 300 to drive and connect the first threaded screw 302 inside the housing. The system host end carried by the sample loading device controls the forward and reverse rotation of the first driving motor 304, which can make the connecting block 303 threadedly connected to the first threaded screw 302 move up or down, so that the culture solution sample loading tube 5 clamped by the clamping mechanism can move up or down.

[0036] In this embodiment, the push-pull mechanism 4 includes a support column 400 vertically arranged on the sample loading platform 1, and a fixing mechanism for locking the syringe barrel of the mineral oil syringe 17 is provided on the side of the support column 400 body. A vertical driving mechanism is provided at the top of the body. The driving end of the vertical driving mechanism is detachably connected to the push cap at the top of the piston rod of the mineral oil syringe 17. The vertical driving mechanism can pull up or push down the piston rod of the mineral oil syringe 17 in the vertical direction through its driving end.

[0037] The fixing mechanism includes a positioning seat 401 and a semicircular clamp 402. The positioning seat 401 is installed on the side wall of the support column 400, and a V-shaped groove is provided on the body thereof. One end of the semicircular clamp 402 is hinged to the positioning seat 401, and the other end is connected to the positioning seat 401 by bolts, so as to lock the injection barrel of the mineral oil syringe 17 at the V-shaped groove on the positioning seat 401. A rubber pad is provided on the arc inner side of the semicircular clamp 402 to contact the outer wall of the injection barrel of the mineral oil syringe 17.

[0038] The vertical drive mechanism includes a bracket 403 disposed at the top of the support column 400, and an axially vertical second threaded screw 404 and a guide rod 405 are arranged between the top of the bracket 403 and the top surface of the support column 400. A connecting plate 406 constituting the driving end of the vertical drive mechanism is threadedly connected to the body of the second threaded screw 404, and the guide rod 405 vertically penetrates the connecting plate 406. A T-slot 407 is provided on the body of the connecting plate 406, into which a push cap at the top of the piston rod of the mineral oil syringe 17 can be horizontally inserted. A second drive motor 408 driving the second threaded screw 404 is also provided on the bracket 403. The system host end carried by the sample loading device controls the second drive motor 408 to work forward and reversely, so that the connecting plate 406 threadedly connected to the second threaded screw 404 can move up or down, thereby realizing the vertical pulling up or pushing down of the piston rod of the mineral oil syringe 17.

[0039] By setting the fixing mechanism and the structural design of the T-slot 407 on the connecting plate 406 , the mineral oil injector 17 can be fixed and disassembled conveniently, thereby facilitating the regular replacement of the mineral oil injector 17 .

[0040] In this embodiment, the inner cavities of multiple conical liquid outlet gun tips 7 are all provided with plugs 8, and a group of plugs 8 protrude from the liquid outlet of the conical liquid outlet gun tip 7. The advantage of such a design is that when the plug 8 protruding from the liquid outlet of the conical liquid outlet gun tip 7 first contacts the inner bottom of the culture dish placed at the semicircular limiting baffle 2 directly below it, the other plugs 8 will also move up synchronously in the corresponding inner cavities of the liquid outlet gun tip 7, so that all the liquid outlets of the liquid outlet gun tip 7 are opened, and the liquid outlet of the liquid outlet gun tip 7 does not need to be very close to the inner bottom of the culture dish to be opened, and a certain distance is maintained to avoid contact, thereby avoiding the phenomenon that the culture fluid cannot flow out due to the contact between the liquid outlet of the liquid outlet gun tip 7 and the inner bottom of the culture dish.

[0041] The above-described implementation modes of the present invention do not constitute a limitation on the protection scope of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A sample loading device, comprising a sample loading platform (1), characterized in that: The sample loading platform (1) is provided with two groups of semicircular limit baffles (2) and a lifting mechanical arm (3) and a push-pull-back mechanism (4) respectively adjacent to the two groups of semicircular limit baffles (2); The driving end of the lifting mechanical arm (3) is provided with a clamping mechanism located directly above the adjacent semicircular limit baffle (2); A culture fluid injection tube (5) is clamped in the clamping mechanism, the top of the culture fluid injection tube (5) is threadedly connected to a first cover body (6), a plurality of conical liquid outlet gun tips (7) are arranged at the bottom of the culture fluid injection tube (5), a plug (8) is arranged in the inner cavity of the conical liquid outlet gun tip (7), and one group of the plugs (8) protrudes from the liquid outlet of the conical liquid outlet gun tip (7), a hollow push plate (9) is arranged inside the culture fluid injection tube (5), a plurality of connecting rods (10) are arranged on the lower end surface of the hollow push plate (9) respectively connected to the plugs (8) in the plurality of conical liquid outlet gun tips (7), and the hollow push plate ( 9) A push rod (11) is provided on the upper end surface, a piston column (12) is provided on the top of the push rod (11), a piston sleeve (13) which is in clearance with the piston column (12) is provided on the inner top surface of the first cover body (6), a spring (14) is provided between the inner top surface of the piston sleeve (13) and the top of the piston column (12), the spring (14) makes the plug (8) close to the liquid outlet of the conical liquid outlet gun head (7) and blocks the liquid outlet of the conical liquid outlet gun head (7) through its own elastic force, and a sample injection tube (15) and an air pipe joint (16) are provided on the side wall of the culture fluid sample injection tube (5); The push-pull mechanism (4) is provided with a mineral oil syringe (17) whose outlet is aligned with the center of the semicircular limit baffle (2) adjacent thereto, and the push-pull mechanism (4) can pull up and push down the piston rod of the mineral oil syringe (17) in the vertical direction.

2. The sample adding device according to claim 1, characterized in that Infrared induction switches (18) are embedded on the arc-shaped inner side walls of the two groups of semicircular limit baffles (2).

3. The sample adding device according to claim 1, characterized in that: The clamping mechanism comprises a sleeve (19) with a notch on the side wall, the sleeve (19) coincides with the axis of the semicircular limit baffle (2) below it, the top of the sleeve (19) is threadedly connected to a second cover body (20), the outer side of the sleeve (19) is connected to the driving end of the lifting mechanical arm (3), the sleeve (19) is covered on the outer periphery of the culture fluid injection tube (5), the notch on the sleeve (19) is used to expose the injection tube (15) and the air pipe joint (16) of the culture fluid injection tube (5), and the bottom end of the sleeve (19) is threadedly connected to an annular cover body (21), as the annular cover body (21) is screwed upward, the culture fluid injection tube (5) is supported and moved upward, so that the top surface of the first cover body (6) on the culture fluid injection tube (5) abuts against the inner side surface of the second cover body (20) at the top of the sleeve (19).

4. The sample adding device according to claim 1, characterized in that The lifting mechanical arm (3) comprises: A square housing (300) is vertically arranged on the sample loading platform (1), and a vertical guide groove (301) is provided on the main body of the housing; A first threaded screw (302) is vertically mounted inside the square housing (300) via a bearing seat, and a connecting block (303) is threadedly connected to the main body of the first threaded screw. The connecting block (303) is slidably connected to the vertical guide groove (301) and protrudes horizontally outside the square housing (300) to form a driving end of the lifting mechanical arm (3); A first driving motor (304) is disposed on the square housing (300) and drives a first threaded screw (302) connected to the interior of the square housing (300).

5. The sample adding device according to claim 1, characterized in that The push-pull mechanism (4) comprises: A support column (400) is vertically arranged on the sample loading platform (1), and a fixing mechanism for locking the injection cylinder of a mineral oil syringe (17) is provided on the side of the body of the support column (400); A vertical driving mechanism is arranged at the top end of the support column (400), and its driving end is detachably connected to a push cap at the top end of the piston rod of the mineral oil syringe (17).

6. The sample adding device according to claim 5, characterized in that The fixing mechanism comprises a positioning seat (401) and a semicircular clamp (402). The positioning seat (401) is installed on the side wall of the support column (400), and a V-shaped groove is provided on the main body thereof. One end of the semicircular clamp (402) is hinged to the positioning seat (401), and the other end is connected to the positioning seat (401) by bolts, so as to lock the injection barrel of the mineral oil syringe (17) at the V-shaped groove on the positioning seat (401). The arc-shaped inner side of the semicircular clamp (402) is provided with a rubber pad in contact with the outer wall of the injection barrel of the mineral oil syringe (17).

7. The sample adding device according to claim 6, characterized in that The vertical driving mechanism comprises: A bracket (403) is disposed at the top of the support column (400); A second axially vertical threaded screw (404) and a guide rod (405) are arranged between the top of the bracket (403) and the top surface of the support column (400); a connecting plate (406) constituting a driving end of a vertical driving mechanism is threadedly connected to the body of the second threaded screw (404); the guide rod (405) vertically penetrates the connecting plate (406); a T-shaped slot (407) is provided on the body of the connecting plate (406) into which a push cap at the top end of a piston rod of a mineral oil syringe (17) can be horizontally inserted; The second driving motor (408) is disposed on the bracket (403) and drives the second threaded screw (404).

8. The sample adding device according to claim 1, characterized in that The air pipe joint (16) is connected to a high-pressure air source device outside the device.

9. The sample adding device according to claim 1, characterized in that The two groups of semicircular limiting baffles (2) on the sample loading platform (1) are respectively used to position the culture dish where the culture liquid droplets need to be made and the culture dish where the mineral oil is added.

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