PCR (Polymerase Chain Reaction) plate sealing film convenient to position

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) plate sealing membrane convenient to position, which comprises a main body, the main body comprises an adaptive frame, a membrane sealing assembly is arranged above the adaptive frame, the membrane sealing assembly comprises a support frame, the lower part of the support frame is of a hollow structure, the inner side wall of the bottom edge of the support frame is provided with a convex plate, the right side of the convex plate is provided with a clamping groove, and the right side of the clamping groove is provided with a clamping groove. A plate sealing film is installed in the clamping groove, and film uncovering strips are fixedly connected to the surface of the periphery of the plate sealing film. Through the structure, the protective layer, the antibacterial layer, the anti-evaporation layer and the adaptive frame are arranged, so that the evaporation loss of a reaction liquid and the mutual pollution between the hole pipes can be effectively prevented, the accuracy of an experiment result is improved, and the device can be repeatedly used after being cleaned by arranging the adaptive frame, so that the experiment cost is saved.

Description

Technical Field

[0001] The utility model relates to the technical field of PCR plate sealing films, in particular to a PCR plate sealing film which is easy to position. Background Art

[0002] PCR (Polymerase Chain Reaction) plates are used for in vitro gene amplification. During the reaction, the samples loaded on the PCR plates need to be heated and cooled continuously. They are widely used in genetics, biochemistry, immunology, medicine and other fields. They are not only used in basic research such as gene separation, cloning and nucleic acid sequence analysis, but also in disease diagnosis or anywhere with DNA and RNA. They are disposable laboratory consumables. In order to avoid evaporation loss of the reaction solution and external impurities falling into the PCR plate, the PCR plate needs to be sealed with a film.

[0003] Currently, most commercially available PCR plates are not designed with film application in mind, which requires operators to be very careful when applying the film, increasing their burden. Utility Model Content

[0004] The utility model provides a PCR plate sealing film which is easy to position. By arranging a protective layer, an antibacterial layer, an anti-evaporation layer and an adapter frame, the evaporation loss of the reaction solution and the mutual contamination between the well tubes can be effectively prevented, the accuracy of the experimental result can be improved, and the adapter frame can be arranged so that the film can be reused after cleaning, thereby saving the experimental cost.

[0005] In order to achieve the above-mentioned purpose, a PCR plate sealing film that is easy to position is provided, including a main body, the main body including an adapter frame, a sealing film assembly is arranged above the adapter frame, the sealing film assembly includes a support frame, the support frame is a hollow structure below and a convex plate is arranged on the inner wall of the bottom edge, a card slot is opened on the right side of the convex plate, a plate sealing film is installed inside the card slot, and a film peeling strip is fixedly connected to the surrounding surface of the plate sealing film. The adapter frame is provided to provide a supporting effect, the support frame is provided to provide a covering effect, the motor is provided to provide a power effect, the convex plate is provided to provide a supporting effect, the card slot is provided to provide a locking effect, the plate sealing film is provided to provide a sealing effect, and the film peeling strip is provided to provide a convenient peeling effect.

[0006] According to the PCR plate sealing film that is easy to position, the plate sealing film is composed of multiple layers of protective materials, and the plate sealing film has a protective layer, a barrier layer, an antibacterial layer, a substrate layer and an anti-evaporation layer built in from top to bottom. The protective layer is provided to provide dustproof effect, the barrier layer is provided to provide blocking effect, the antibacterial layer is provided to provide anti-invasion effect of harmful substances, the substrate layer is provided to provide connection and support effect, and the anti-evaporation layer is provided to provide liquid volatilization prevention effect.

[0007] According to the PCR plate sealing film that is easy to position, a motor is fixedly connected to the top of the support frame, and the output end of the motor passes through the top of the support frame and is fixedly connected to a pressing plate. The pressing plate is provided to provide a pressing effect.

[0008] According to the PCR plate sealing film that is easy to position, the surface of the adapter frame is provided with a plurality of circular holes arranged in a linear array, and a sampling plate is installed inside the adapter frame through the circular holes. The surface of the adapter frame is provided with a plurality of circular holes arranged in a linear array in order to adapt to a liquid loading container.

[0009] According to the PCR plate sealing film that is easy to position, the width of the support frame is slightly larger than the width of the adapter frame, and the length of the support frame is slightly longer than the length of the adapter frame. By setting the width of the support frame to be slightly larger than the width of the adapter frame, and the length of the support frame to be slightly longer than the length of the adapter frame, the pressure plate can fully contact the surface of the adapter frame when pressing downward.

[0010] According to the PCR plate sealing film that is easy to position, the film peeling strips are all located outside the upper surface of the adapter frame, ensuring that the plate sealing film is in full contact with the upper surface of the adapter frame.

[0011] According to the PCR plate sealing film that is easy to position, the bottom of the pressing plate is smooth and has a large area, which can fully contact the upper surface of the adapter frame to press the plate sealing film. The bottom of the pressing plate is set to be smooth to prevent the rough surface of the plate sealing film from being easily damaged during pressure.

[0012] The beneficial effects of the utility model include: by providing a protective layer, an antibacterial layer, an anti-evaporation layer, and an adapter frame, the evaporation loss of the reaction liquid and the mutual contamination between the orifice tubes can be effectively prevented, the accuracy of the experimental results can be improved, and by providing an adapter frame, the device can be reused after cleaning, thus saving the experimental cost.

[0013] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0015] Figure 1 This is a schematic diagram of the overall structure of a PCR plate sealing membrane that is easy to position according to the utility model;

[0016] Figure 2 It is a side view of the film sealing assembly of the utility model;

[0017] Figure 3 It is a schematic diagram of the cross-sectional structure of the sealing film of the utility model;

[0018] Figure 4 It is a schematic diagram of the top view of the film sealing component of the utility model.

[0019] Legend:

[0020] 1. Main body; 11. Adapter frame; 12. Sampling plate;

[0021] 2. Sealing film assembly; 21. Support frame; 22. Motor; 23. Pressing plate; 24. Convex plate; 25. Card slot; 26. Sealing film; 261. Protective layer; 262. Barrier layer; 263. Antibacterial layer; 264. Base material layer; 265. Anti-evaporation layer; 27. Film peeling strip. DETAILED DESCRIPTION

[0022] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0023] Reference Figures 1 to 4 The utility model embodiment provides a PCR plate sealing film that is easy to position, which includes a main body 1, the main body 1 includes an adapter frame 11, a sealing film assembly 2 is arranged above the adapter frame 11, and the sealing film assembly 2 includes a support frame 21, a motor 22 is fixedly connected to the top of the support frame 21, and the output end of the motor 22 passes through the top of the support frame 21 and is fixedly connected to a pressing plate 23. The bottom of the pressing plate 23 is smooth and has a large area, which can fully contact the upper surface of the adapter frame 11 to apply pressure to the sealing film 26. The pressing plate 23 adopts a molded plate, and during the manufacturing process, a layer of polyvinyl chloride (PVC) film will be adsorbed on the MDF through surface treatment technology. This treatment method not only gives the molded plate waterproof and environmentally friendly characteristics, but also makes it have a variety of colors and textures, as well as excellent impact resistance and dimensional stability.

[0024] The support frame 21 has a hollow structure below and a convex plate 24 is provided on the inner side wall of the bottom edge. A card slot 25 is provided on the right side of the convex plate 24. A sealing film 26 is installed inside the card slot 25. Film strips 27 are fixedly connected to the surrounding surfaces of the sealing film 26. The film strips 27 are distributed on the four side surfaces of the sealing film 26. Two groups are provided on the width surface and one group of longer sealing strips is provided on the length surface. When the machine is pressed downward, it is ensured that the sealing film 26 can be closely adhered to the solution port on the sampling plate 12. The film strips 27 are all located outside the upper surface of the adapter frame 11 to ensure that the sealing film 26 is in full contact with the upper surface of the adapter frame 11.

[0025] The sealing film 26 is composed of a multi-layer material protection, and the sealing film 26 is built with a protective layer 261, a barrier layer 262, an antibacterial layer 263, a substrate layer 264 and an anti-evaporation layer 265 from top to bottom. The protective layer 261 usually uses polyethylene, polypropylene and other materials as the sealing layer material, which has the properties of high temperature resistance, corrosion resistance, crack resistance, solvent resistance and the like. The antibacterial layer 263 is prepared by making the antibacterial agent into an antibacterial masterbatch, and is prepared by extruding or drooling the antibacterial agent or antibacterial masterbatch and a resin monolayer, or by extruding it together as a surface layer and other layers to form an antibacterial composite film. The anti-evaporation layer 265 is also a film-like material made of polyethylene, thereby slowing down the evaporation rate of water, playing a role in retaining water and reducing the amount of water. It can prevent oxygen from entering the interior of the object and reduce the contact with oxygen, thereby playing a role in preventing oxidation and degradation.

[0026] The surface of the adapter frame 11 is provided with a plurality of circular holes arranged in a linear array, and a sampling plate 12 is installed inside the adapter frame 11 through the circular holes. The width of the support frame 21 is slightly larger than the width of the adapter frame 11, and the length of the support frame 21 is slightly longer than the length of the adapter frame 11. The adapter frame 11 is made of organic glass material, which is a transparent plastic material with good weather resistance and impact resistance.

[0027] Working principle: When in use, place the sealing film 26 inside the card slot 25, align the support frame 21 with the surface of the adapter frame 11 and place it on top, start the motor 22, drive the pressure plate 23 to squeeze downward, and due to the effect of force, the sealing film 26 is in close contact with the solution port part of the sampling plate 12 until it is tightly adhered, remove the support frame 21, and peel it open with the peeling strip 27, so that the sampling solution is well preserved.

[0028] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A PCR plate sealing film that is easy to position, characterized in that: The invention comprises a main body (1), wherein the main body (1) comprises an adapting frame (11), a sealing film assembly (2) is arranged above the adapting frame (11), the sealing film assembly (2) comprises a supporting frame (21), the supporting frame (21) has a hollow structure below and a convex plate (24) is arranged on the inner wall of the bottom edge, a card slot (25) is arranged on the right side of the convex plate (24), a sealing film (26) is installed inside the card slot (25), and the sealing film (26) is fixedly connected to the surrounding surface with a film peeling strip (27).

2. A PCR plate sealing film that is easy to position according to claim 1, characterized in that: The plate sealing film (26) is composed of a plurality of layers of protective materials, and the plate sealing film (26) has a protective layer (261), a barrier layer (262), an antibacterial layer (263), a substrate layer (264) and an anti-evaporation layer (265) built therein from top to bottom.

3. A PCR plate sealing film that is easy to position according to claim 1, characterized in that: A motor (22) is fixedly connected to the top of the support frame (21), and an output end of the motor (22) passes through the top of the support frame (21) and is fixedly connected to a pressing plate (23).

4. A PCR plate sealing film that is easy to position according to claim 1, characterized in that: The surface of the adapter frame (11) is provided with a plurality of circular holes arranged in a linear array, and a sampling plate (12) is installed inside the adapter frame (11) through the circular holes.

5. The PCR plate sealing film that is easy to position according to claim 1, characterized in that: The width of the support frame (21) is greater than the width of the adapter frame (11), and the length of the support frame (21) is greater than the length of the adapter frame (11).

6. The PCR plate sealing film that is easy to position according to claim 1, characterized in that: The film peeling strips (27) are all located outside the upper surface of the adapter frame (11), ensuring that the sealing film (26) is in full contact with the upper surface of the adapter frame (11).

7. A PCR plate sealing film that is easy to position according to claim 3, characterized in that: The bottom of the pressing plate (23) is smooth and has an area sufficient to contact the upper surface of the adapter frame (11) to apply pressure to the sealing film (26).

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