Device convenient for microscopic observation of human dental pulp mesenchymal stem cell lysis process
By designing a microscope observation device including a slide limit assembly, the sample contamination problem caused by inadequate extrusion of the slide is solved, and a more accurate microscope observation result is achieved.
Patent Information
- Application Number
- CN202423264421.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2034-12-30
AI Technical Summary
During the lysis of human pulp mesenchymal stem cells by microscope, if the slides on the upper and lower sides of the sample are not squeezed in place, it may lead to air entering between the slides, resulting in contamination of the sample, which will affect the test effect.
A device is designed including a base, an operating cavity, a slide limit assembly, a vertical height adjustment assembly, an operating assembly and an observation assembly. The slide limiting assembly achieves a tight fit between the slide through the combination of slide rod, spring and extrusion plate to prevent air from entering.
Through this device, air inflow between the slides is effectively avoided, sample contamination is prevented, and the accuracy of microscope observation results is improved.
Smart Images
Figure CN222439760U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of optical instruments, and in particular to a device that facilitates observation of a human dental pulp mesenchymal stem cell lysis process under a microscope. Background Art
[0002] Human dental pulp mesenchymal stem cells, also known as dental pulp stem cells, are adult stem cells that exist in the pulp cavity of human teeth. They are a type of pluripotent stem cells that have the ability to self-renew and differentiate into a variety of cell types, including nerve cells, bone cells, fat cells, chondrocytes, cardiomyocytes, etc.
[0003] Microscopes are commonly used on the market to observe cells. However, during the preparation of cell samples, if the upper and lower glass slides outside the sample are not squeezed into place, air may enter between the glass slides, which may cause the sample to be contaminated, which may lead to erroneous observation results and affect the experimental effect. Utility Model Content
[0004] The utility model provides a device that is convenient for observing the lysis process of human dental pulp mesenchymal stem cells under a microscope, which solves the problem in the related art that if the upper and lower glass slides outside the sample are not squeezed into place, air may enter between the glass slides, which may cause the sample to be contaminated, and further may cause errors in the observation results and affect the test effect.
[0005] The technical solution of the utility model is as follows: a device for observing the lysis process of human dental pulp mesenchymal stem cells under a microscope, comprising: a base, an operating cavity is arranged inside the base, a glass slide limiting assembly is arranged at the top of the inner part of the operating cavity, which is used to limit the sample glass slide, a vertical height adjustment assembly is arranged at the lower end of the glass slide limiting assembly, which is used to adjust the vertical height of the glass slide limiting assembly, an operating assembly is arranged on one side of the vertical height adjustment assembly, which is used to horizontally adjust the glass slide limiting assembly, and an observation assembly is arranged at the top of the base for observing the sample.
[0006] The slide limiting assembly includes a limiting frame arranged inside the operating cavity, the four corners of the inner bottom end of the limiting frame are fixedly connected with sliding sleeves, the inner wall of each sliding sleeve is slidably connected with a sliding rod, the outside of each sliding sleeve is sleeved with a spring, one end of each spring is fixedly connected to the inner bottom end of the limiting frame, the top ends of the four sliding rods are fixedly connected with an extrusion plate, and the top end of each spring is fixedly connected to the extrusion plate.
[0007] Preferably, the slide limiting assembly includes an observation port extending through the top of the limiting frame, and a placement port extending through one side of the limiting frame.
[0008] Preferably, the vertical height adjustment assembly includes hydraulic rods fixedly connected to four corners of the bottom end of the limiting frame, and the bottom ends of the four hydraulic rods are fixedly connected to a bottom plate.
[0009] Preferably, the vertical height adjustment assembly further comprises sliding blocks fixedly connected to both sides of the bottom end of the base plate, and two sliding grooves are provided on the inner wall of the bottom end of the base, and each of the sliding grooves is slidably connected to the sliding blocks.
[0010] Preferably, the operating assembly comprises a motor fixedly connected to the inner wall of one end of the limiting frame, the output end of the motor is fixedly connected to a screw rod, and the other end of the screw rod is rotatably connected to the inner wall of the limiting frame.
[0011] Preferably, the operating assembly further comprises a threaded sleeve which is connected to the outer wall of the screw rod, one end of the outer wall of the threaded sleeve is fixedly connected to an L-shaped connecting rod, and the other end of the L-shaped connecting rod is fixedly connected to the outer wall of the base plate.
[0012] Preferably, the observation assembly includes two slide rails opened on the outer wall of the top end of the base, the inner wall of each of the slide rails is slidably connected to a T-shaped slide seat, and the top ends of the two T-shaped slide seats are fixedly connected to a microscope.
[0013] Preferably, an opening is formed through the outer wall of the top end of the base, and the opening is communicated with the interior of the operating cavity.
[0014] The working principle and beneficial effects of the utility model are:
[0015] In the utility model, the squeezing plate is moved downward, the spring is squeezed by the squeezing plate, and then the glass slide with the sample is placed on the upper end of the squeezing plate, and then the spring rebounds, thereby driving the slide bar to slide upward, and the squeezing plate is driven to move upward by the slide bar, and the glass slide is driven to move upward by the squeezing plate, so that the glass slide is limited at the inner top end of the limit frame, and the sample inside the glass slide is observed through the observation port of the limit frame, so that it is convenient to squeeze the glass slides on both sides of the sample, so that the glass slides on both sides of the sample are more closely attached together, thereby preventing air from entering the inner side of the glass slide, preventing the sample from being contaminated, and further improving the accuracy of the results of subsequent observations. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] The utility model is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.
[0017] Figure 1 This is a front view structural schematic diagram of the utility model;
[0018] Figure 2 This is a schematic diagram of the front cross-sectional structure of the utility model;
[0019] Figure 3This is a schematic diagram of the cross-sectional structure of the glass slide limiting assembly proposed in the utility model;
[0020] Figure 4 This is a structural schematic diagram of the glass slide limiting assembly proposed in the utility model.
[0021] In the figure: 1. base; 2. operating chamber; 3. slide limit assembly; 301. limit frame; 302. slide rod; 303. sleeve; 304. spring; 305. extrusion plate; 306. observation port; 307. placement port; 4. vertical height adjustment assembly; 401. hydraulic rod; 402. bottom plate; 403. slider; 404. slide groove; 5. operating assembly; 501. motor; 502. lead screw; 503. threaded sleeve; 504. L-shaped connecting rod; 6. observation assembly; 601. microscope; 602. T-type slide; 603. slide rail; 7. opening. DETAILED DESCRIPTION
[0022] The following will be combined with the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0023] The present application embodiment discloses a device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells. Figure 1-Figure 4, a device for observing the lysis process of human dental pulp mesenchymal stem cells under a microscope, comprising: a base 1, an operating chamber 2 is arranged inside the base 1, a glass slide limiting assembly 3 is arranged at the top of the inner part of the operating chamber 2, which is used to limit the sample glass slide, a vertical height adjustment assembly 4 is arranged at the lower end of the glass slide limiting assembly 3, which is used to adjust the vertical height of the glass slide limiting assembly 3, an operating assembly 5 is arranged on one side of the vertical height adjustment assembly 4, which is used to horizontally adjust the glass slide limiting assembly 3, an observation assembly 6 is arranged at the top of the base 1 for observing the sample, the glass slide limiting assembly 3 comprises a limiting frame 301 arranged inside the operating chamber 2, the four corners of the inner bottom end of the limiting frame 301 are fixedly connected with sliding sleeves 303, the inner wall of each sliding sleeve 303 is slidably connected with a sliding rod 302, and the combination of the sliding sleeve 303 and the sliding rod 302 is spring 30 4 is used for limiting to avoid deviation during the squeezing process, the outside of each sliding sleeve 303 is sleeved with a spring 304, one end of each spring 304 is fixedly connected to the inner bottom end of the limiting frame 301, the top ends of the four sliding rods 302 are fixedly connected with a squeezing plate 305, the top ends of each spring 304 are fixedly connected to the squeezing plate 305, the outer side of the squeezing plate 305 is fixedly connected with a handle, which is convenient for the user to move the squeezing plate 305, the glass slide limiting assembly 3 includes an observation port 306 that is opened through the top end of the limiting frame 301, the diameter of the observation port 306 is smaller than the squeezing plate 305, which is convenient for limiting the squeezing plate 305 to the inner side of the limiting frame 301, thereby facilitating limiting the glass slide to the inner top end of the limiting frame 301, and then facilitating observation of the sample through the observation port 306, and a placement port 307 is opened through one side of the limiting frame 301.
[0024] See also Figure 2 and Figure 3The vertical height adjustment component 4 includes hydraulic rods 401 fixedly connected to the four corners of the bottom end of the limit frame 301, and the bottom ends of the four hydraulic rods 401 are fixedly connected to the bottom plate 402. By adjusting the height of the hydraulic rods 401, the height of the limit frame 301 is adjusted, so that the limit frame 301 can be moved upward out of the opening 7, so as to facilitate the sample to approach the lens of the microscope 601. The vertical height adjustment component 4 also includes sliders 403 fixedly connected to both sides of the bottom end of the bottom plate 402. Two slide grooves 404 are provided on the inner wall of the bottom end of the base 1, and each slide groove 404 is slidably connected to the slider 403. The operating component 5 includes a motor 501 fixedly connected to the inner wall of one end of the limit frame 301, and the output end of the motor 501 is fixedly connected to the screw rod 502. The screw rod 5 The other end of 02 is rotatably connected to the inner wall of the limit frame 301. When the screw rod 502 rotates, the screw rod 502 drives the threaded sleeve 503 to transmit, and the threaded sleeve 503 drives the L-shaped connecting rod 504 to transmit, and the L-shaped connecting rod 504 drives the bottom plate 402 to transmit, and the bottom plate 402 drives the slider 403 to slide on the slide groove 404, thereby driving the bottom plate 402 to move, thereby driving the limit frame 301 to move, and then driving the sample to move. The operating component 5 also includes a threaded sleeve 503 connected to the outer wall of the screw rod 502, and the outer wall of one end of the threaded sleeve 503 is fixedly connected to the L-shaped connecting rod 504, and the other end of the L-shaped connecting rod 504 is fixedly connected to the outer wall of the bottom plate 402.
[0025] See also Figure 1 and Figure 2 The observation component 6 includes two slide rails 603 opened on the outer wall of the top of the base 1, and the inner wall of each slide rail 603 is slidably connected with a T-shaped slide seat 602. The top of the two T-shaped slide seats 602 is fixedly connected with a microscope 601. The microscope 601 is the existing technology. Handles are fixedly connected on both sides of the bottom of the microscope 601 to facilitate the user to move the microscope 601. An opening 7 is opened through the outer wall of the top of the base 1. The inner wall of the opening 7 is slidably connected to the outer wall of the limit frame 301 to facilitate moving the limit frame 301 out of the opening 7. The opening 7 is connected to the inside of the operating cavity 2.
[0026] Working principle and use process: Press the squeezing plate 305 downward, and the squeezing plate 305 drives the slide bar 302 to move downward on the inner side of the sliding sleeve 303, so that the spring 304 is compressed, and then the glass slide with the sample is placed on the squeezing plate 305 from the placement port 307, and then the pressure on the squeezing plate 305 is removed, so that the spring 304 begins to restore the elastic force, thereby driving the slide bar 302 to move upward, and the squeezing plate 305 is driven to move upward by the slide bar 302, so that the sample is driven upward by the squeezing plate 305, so that the sample is limited to the inner side of the top of the limit frame 301, and then the motor 501 is started, and the screw rod 502 is driven to rotate by the output end of the motor 501. The threaded sleeve 503 is driven by the screw rod 502 for transmission, the L-shaped connecting rod 504 is driven by the threaded sleeve 503 for transmission, the bottom plate 402 is driven by the L-shaped connecting rod 504 for transmission, the slider 403 is driven by the bottom plate 402 to move on the slide groove 404, thereby driving the limit frame 301 to move to the bottom of the opening 7, and the microscope 601 is moved. The T-shaped slide 602 is driven by the microscope 601 to move toward the opening 7 on the slide rail 603, so that the lens of the microscope 601 is located above the opening 7, and then the hydraulic rod 401 is started, and the limit frame 301 is driven to move upward through the output end of the hydraulic rod 401, thereby driving the sample close to the lens of the microscope 601.
[0027] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A device for microscopically observing the lysis process of human dental pulp mesenchymal stem cells, comprising: The base (1) is characterized in that an operating cavity (2) is provided inside the base (1), a glass slide limiting assembly (3) is provided at the top of the operating cavity (2) for limiting the position of the sample glass slide, a vertical height adjustment assembly (4) is provided at the lower end of the glass slide limiting assembly (3) for adjusting the vertical height of the glass slide limiting assembly (3), an operating assembly (5) is provided on one side of the vertical height adjustment assembly (4) for adjusting the horizontal position of the glass slide limiting assembly (3), and an observation assembly (6) is provided at the top of the base (1) for observing the sample; The slide limiting assembly (3) comprises a limiting frame (301) arranged inside the operating chamber (2); the four corners of the inner bottom end of the limiting frame (301) are fixedly connected to sliding sleeves (303); the inner wall of each sliding sleeve (303) is slidably connected to a sliding rod (302); the outside of each sliding sleeve (303) is sleeved with a spring (304); one end of each spring (304) is fixedly connected to the inner bottom end of the limiting frame (301); the top ends of the four sliding rods (302) are fixedly connected to a squeezing plate (305); and the top end of each spring (304) is fixedly connected to the squeezing plate (305).
2. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The slide limiting assembly (3) comprises an observation port (306) extending through the top of the limiting frame (301), and a placement port (307) extending through one side of the limiting frame (301).
3. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The vertical height adjustment assembly (4) comprises hydraulic rods (401) fixedly connected to the four corners of the bottom end of the limiting frame (301), and the bottom ends of the four hydraulic rods (401) are fixedly connected to a bottom plate (402).
4. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 3, characterized in that: The vertical height adjustment component (4) further comprises sliding blocks (403) fixedly connected to both sides of the bottom end of the base plate (402); the inner wall of the bottom end of the base (1) is provided with two sliding grooves (404); each of the sliding grooves (404) is slidably connected to the sliding blocks (403).
5. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 4, characterized in that: The operating assembly (5) comprises a motor (501) fixedly connected to the inner wall of one end of the limiting frame (301), the output end of the motor (501) being fixedly connected to a screw rod (502), and the other end of the screw rod (502) being rotatably connected to the inner wall of the limiting frame (301).
6. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 5, characterized in that: The operating assembly (5) further comprises a threaded sleeve (503) which is connected to the outer wall of the screw rod (502); an L-shaped connecting rod (504) is fixedly connected to the outer wall of one end of the threaded sleeve (503); and the other end of the L-shaped connecting rod (504) is fixedly connected to the outer wall of the bottom plate (402).
7. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The observation assembly (6) comprises two slide rails (603) provided on the outer wall at the top end of the base (1), the inner wall of each slide rail (603) being slidably connected to a T-shaped slide seat (602), and the top ends of the two T-shaped slide seats (602) being fixedly connected to a microscope (601).
8. The device for facilitating microscopic observation of the lysis process of human dental pulp mesenchymal stem cells according to claim 1, characterized in that: An opening (7) is formed through the outer wall of the top end of the base (1), and the opening (7) is communicated with the interior of the operating cavity (2).