Culture device suitable for cell migration experiment

Abstract

The utility model discloses a culture device suitable for a cell migration experiment, the bottom of the inner wall of a culture dish is divided into a scratch area and two inclined bottom areas, and the scratch area is of a strip-shaped plane structure, is used for observing the growth condition of cells and is arranged in the middle; the two inclined bottom areas are located on the left side and the right side of the scratch area and incline downwards relative to the scratch area, and two containing grooves for containing cell suspension are formed by the two inclined bottom areas. By the adoption of the scheme, cost is low, efficiency is high, operation is simple and easy, sample adding is not affected, damage to cells by external instruments is avoided, the culture dish can be used for culturing the cells, scratched areas can be easily manufactured, and instrument cost is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to a culture device suitable for cell migration experiments. Background Art

[0002] The basic principle of the cell scratch test is to artificially create a blank area on the fused monolayer of cells, called a "scratch / wound", and the cells at the edge will gradually enter the blank area to heal the "scratch / wound". Because it is similar to the in vitro wound healing process, it is also called the wound healing test and is widely used to observe the effects of exogenous factors such as drugs and genes on cell migration and repair.

[0003] To create a blank area on a confluent cell monolayer, there are two approaches:

[0004] One method is to use the gun tip to make scratches artificially. First, use a marker pen to draw horizontal lines on the back of the container (such as a 6-well plate) with a ruler, approximately one line every 0.5 to 1 cm, across the holes, and draw at least 3 lines in each hole. Then turn the container over, and after the cells have merged, use the gun tip to lean against the ruler on one side and scratch as perpendicularly as possible to the horizontal lines on the back to create a blank area.

[0005] The first method has the following defects: too many equipments are required (ruler, marker), the steps are cumbersome, and the line needs to be drawn on the back first, and then turned over to draw vertically on the front; in the cell scratch experiment, it is necessary to lay the adhesion matrix (such as Laminin coating) on ​​the bottom of the container in advance, wait for the cells to grow (fusion), and operate under the premise of containing a certain volume of culture medium inside. Due to the resistance of the adhesion matrix, cells and liquid coated on the bottom, only one side of the ruler is used as an aid. At this time, the position of the gun tip cannot be accurately fixed, and the strength cannot be controlled, which will cause the cells to deviate in a certain direction or fail to present a straight line, resulting in uneven scratch width, and it is difficult to ensure consistency and poor repeatability, resulting in unsatisfactory experimental results; this operation mainly produces a blank area by squeezing and pushing the outer wall of the gun tip. The inside of the gun tip is hollow. When moving, the same surface will be damaged twice, and due to uneven force control, the bottom adhesion matrix and the cells attached to it will be damaged. Since cells need to adhere to the bottom matrix, if the coating matrix on the surface is damaged, the result will be uncontrollable, which will lead to unsatisfactory scratch test results.

[0006] Another method is to use a cell plug-in. In a container with or without a coated bottom, before cell inoculation, a cell plug-in is placed, and then an appropriate amount of cell suspension is added. After the cells adhere to the wall and fill the plug-in, a blank area can be formed after the plug-in is removed.

[0007] The second method has the following defects: if the clamping instruments such as tweezers are not completely disinfected, cell contamination will occur; if the experimental cells are suspended cells, they need to be pre-coated at the bottom of the container, then sliding will occur when placing the plug-in, resulting in poor adhesion, thus affecting subsequent sample addition and resulting in unsatisfactory experimental results; the plug-in has a small caliber, which makes it difficult for technicians to operate, and the gun tip may accidentally touch the plug-in during the sample addition process, causing the plug-in to slip, thereby causing leakage of the cell suspension and resulting in unsatisfactory experimental results; during the removal of the plug-in, the bottom may become sticky, resulting in uneven or torn edges of the blank area; the price is expensive, and the product is not recommended for reuse, so the experimental cost is high. Utility Model Content

[0008] The utility model aims to provide a culture device suitable for cell migration experiments, which has low cost, high efficiency, is simple and easy to operate, does not affect sample addition, and avoids damage to cells by external instruments.

[0009] The purpose of the utility model is achieved as follows: a culture device suitable for cell migration experiments, comprising a transparent cell culture plate provided with a plurality of circular culture dishes, wherein the bottom of the inner wall of the culture dishes is divided into a scratch area and two oblique bottom areas;

[0010] The scratched area is set as a strip-shaped plane structure and is used to observe cell growth, and is arranged in the center, and the scratched areas of all culture dishes are parallel to each other;

[0011] The two oblique bottom areas are located at the left and right sides of the scratch area and are inclined downward relative to the scratch area. The two oblique bottom areas form two receiving grooves for receiving the cell suspension.

[0012] Furthermore, it also includes a bottom support that can be tilted up and down at a certain angle, and the cell culture plate is placed on the bottom support; when the cell suspension is injected into the two inclined bottom areas in sequence, the bottom support is adjusted to form two inclined angles of the cell culture plate so that the two inclined bottom areas are respectively in a horizontal state.

[0013] Furthermore, each culture dish is provided with a transparent protection chamber for protecting the scratched area, and the protection chamber is provided as a protection cover structure, and when in use, the protection chamber covers and seals the scratched area.

[0014] Furthermore, the scratch area has two long sides parallel to each other, and the two long sides of the scratch area are respectively connected to the two inclined bottom areas. The scratch area is provided with two protection bin positioning grooves respectively located at its two ends. The protection bin is a rectangular box-shaped structure as a whole and is parallel to the scratch area. The two short side plates of the protection bin are respectively inserted into the two protection bin positioning grooves to position the protection bin at the position of the scratch area.

[0015] Furthermore, the bottom of the bottom support is connected to two groups of movable legs distributed left and right so that it can be adjusted by rotating up and down, and each group of movable legs has at least one. When the cell culture plate is driven to tilt to a predetermined angle, one group of movable legs is in an open state and supports the placement table, so that the bottom support and the cell culture plate are in a tilted state.

[0016] Furthermore, each movable foot is provided with a foot receiving groove for receiving the movable foot, and the movable foot is connected to the foot receiving groove for being rotatable up and down. The foot receiving groove is arranged at the bottom of the bottom support. When the bottom support and the cell culture plate are laid flat, all movable feet are in the foot receiving groove.

[0017] Furthermore, both short side plates of the protection bin are connected with hanging ears, and a pair of notches for fixing the hanging ears are provided on the upper edge of the side wall of the culture dish, and the hanging ears are plugged into the notches.

[0018] Furthermore, the bottom surface of the cell culture plate is provided with a plurality of scratch scale areas for measurement, which are matched one by one with the scratch areas, each scratch scale area is provided with a plurality of scratch area scale lines, and each scratch scale area is directly opposite to the scratch area of ​​the corresponding culture dish.

[0019] The beneficial effects of the utility model are:

[0020] 1. Divide the bottom of the inner wall of the culture dish into a scratch area and two oblique bottom areas. Add the cell suspension only in the two oblique bottom areas. The scratch area is at the highest position and can be easily reserved as the scratch area required during the experiment.

[0021] 2. Low cost, high efficiency, simple and easy to operate, no impact on sample addition, avoid damage to cells by external instruments, the culture dish can be used to culture cells and also to easily create scratch areas, reducing equipment costs;

[0022] 3. Due to the setting of the protection chamber, the scratch area can be protected during the process of adding the cell suspension to prevent the cell suspension from flowing to the scratch area when adding the cell suspension;

[0023] 4. The bottom surface area of ​​the culture dish can be expanded, thereby expanding the culture area. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of a culture dish of the present utility model.

[0025] Figure 2 This is an overhead view of the cell culture plate.

[0026] Figure 3 is a schematic diagram of the bottom surface of a cell culture plate.

[0027] Figure 4 This is a schematic diagram of the use of the protection chamber.

[0028] Figure 5 This is a schematic diagram of the installation of the protection chamber.

[0029] Figure 6 This is a comparison diagram of the tilted state of the cell culture plate.

[0030] Figure 7 This is a schematic diagram of adding cell suspension to the culture dish.

[0031] Figure 8 This is a schematic diagram of the cover plate setting. DETAILED DESCRIPTION

[0032] The following will be combined with the attached embodiment of the utility model Figure 1-8 , clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] like Figure 1 , 2 As shown in , 8, based on the existing cell culture plate (such as the patent application document with publication number CN113278523A attached Figure 1 A cell culture plate and its text content), a culture device suitable for cell migration experiment is proposed, comprising:

[0034] A cell culture plate 2 having a plurality of culture dishes 201, the cell culture plate 2 being a professional device commonly used in the field of biological experiments, and the number of the culture dishes 201 is not limited;

[0035] The cover plate 1 is used to cover the upper side of the cell culture plate 2, such as Figure 8 As shown, all culture dishes 201 are covered to close the culture space so that the culture space is isolated from the external environment.

[0036] The bottom of the inner wall of the culture dish 201 is divided into a scratch area 201a and two slanted bottom areas 201b.

[0037] The scratched area 201a is configured as a strip-shaped plane structure and is used to observe cell growth. It is centrally arranged to facilitate observation and calculation of the position and speed of cell migration. The scratched areas 201a of all culture dishes 201 are parallel to each other.

[0038] The two inclined bottom areas 201b are located on the left and right sides of the scratch area 201a and are inclined downward relative to the scratch area 201a. The inclination angle can be 30° or other angles. The two inclined bottom areas 201b form two receiving grooves for receiving the cell suspension 6.

[0039] As a preferred embodiment, Figure 3 As shown, the bottom surface 204 of the cell culture plate 2 is provided with a plurality of scratch scale areas for measurement, which are matched one by one with the scratch areas 201a. Each scratch scale area is provided with a plurality of scratch area scale lines 203. Since the cell culture plate 2 is transparent (this is common knowledge), it is convenient for the experimenter to observe the cell growth progress of the scratch area 201a. Each scratch scale area is opposite to the scratch area 201a of the corresponding culture dish 201.

[0040] As a preferred embodiment, Figure 6 , 7 As shown, the device also includes a bottom support 3 that can be tilted up and down at a certain angle, and the cell culture plate 2 is placed on the bottom support 3; when the cell suspension 6 is injected into the two inclined bottom areas 201b in turn, the bottom support 3 is adjusted to form two inclined angles of the cell culture plate 2, so that the two inclined bottom areas 201b are respectively in a horizontal state, which facilitates the cells to evenly settle on the surface of the inclined bottom area 201b during the addition of the cell suspension to avoid uneven distribution.

[0041] As a preferred embodiment, Figure 4 , 5 As shown, each culture dish 201 is provided with a transparent protective chamber 5 for protecting the scratched area 201a. The protective chamber 5 is configured as a protective cover structure. When in use, the protective chamber 5 covers and seals the scratched area 201a. The protective chamber 5 protects the scratched area 201a when adding samples to prevent the cell suspension from touching the scratched area 201a and affecting the experimental effect. The protective chamber 5 can be slightly larger so as to cover the scratched area 201a.

[0042] As a preferred embodiment, Figure 1 , 4 As shown in Figure 5, the scratch area 201a has two long sides parallel to each other, and the two long sides of the scratch area 201a are respectively connected to the two inclined bottom areas 201b. The scratch area 201a is provided with two protection bin positioning grooves 201c respectively located at the two ends thereof. The protection bin 5 is a rectangular box-shaped structure as a whole and is parallel to the scratch area 201a. The two short side plates of the protection bin 5 are respectively inserted into the two protection bin positioning grooves 201c to position the protection bin 5 at the position of the scratch area 201a.

[0043] As a preferred embodiment, Figure 6 , 7As shown, the bottom of the bottom support 3 is connected to two groups of movable legs 4 distributed left and right so as to be adjustable and rotatable up and down, and each group of movable legs 4 has at least one. When the cell culture plate 2 is driven to tilt to a predetermined angle (for example, 30°), one group of movable legs 4 is in an open state and supports the placement table, so that the bottom support 3 and the cell culture plate 2 are in a tilted state (for example, 30°).

[0044] according to Figure 2 It can be seen that the scratched areas 201a of all culture dishes 201 are parallel to each other and parallel to two short sides of the rectangular cell culture plate 2. When it is necessary to drive the two inclined bottom areas 201b of the culture dishes 201 to be in a horizontal state in turn, it is necessary to drive the bottom support 3 and its cell culture plate 2 to tilt 30° left and right in turn, so that the two inclined bottom areas 201b of each culture dish 201 are in a horizontal state in turn. The inclined bottom areas 201b in a horizontal state are conducive to the uniform distribution of the cell suspension.

[0045] like Figure 6 As shown, in order to facilitate the control of the tilt angle of the bottom support 3 and its cell culture plate 2, each movable leg 4 is provided with a leg receiving groove 3a for receiving the movable leg 4, and the movable leg 4 is connected to the leg receiving groove 3a so as to rotate up and down, and the rotation fulcrum is close to the edge of the leg receiving groove 3a. After the movable leg 4 is opened, the movable leg 4 often rests against the edge of the leg receiving groove 3a (similar to the bottom leg of a computer keyboard). The leg receiving groove 3a is provided at the bottom of the bottom support 3. When the bottom support 3 and the cell culture plate 2 are laid flat, all the movable legs 4 are in the leg receiving groove 3a so as to lay the cell culture plate 2 flat.

[0046] When making one of the inclined bottom areas 201b of the culture dish 201 in a horizontal state, the movable foot 4 corresponding to the inclined bottom area 201b needs to be opened and supported on the placement table, while the other movable foot 4 is not opened, so that the bottom support 3 and its cell culture plate 2 are at a predetermined tilt angle, so that the corresponding inclined bottom area 201b is in a horizontal state; when driving the other inclined bottom area 201b of the culture dish 201 to be in a horizontal state, the other movable foot 4 is opened, and the previously opened movable foot 4 is folded, so that the bottom support 3 and its cell culture plate 2 are at another predetermined tilt angle, so that the corresponding inclined bottom area 201b is in a horizontal state.

[0047] As a preferred embodiment, Figure 5As shown, in order to better position the protection chamber 5, the two short side plates of the protection chamber 5 are connected with hanging ears 5a, that is, the protection chamber 5 is provided with two hanging ears 5a, and a pair of notches 202 for fixing the hanging ears 5a are opened on the upper edge of the side wall of the culture dish 201, and the two hanging ears 5a are respectively inserted into the two notches 202. The protection chamber 5 can be removed after the addition of the cell suspension is completed, so that the cells can grow in the scratch area 201a.

[0048] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "front", "back", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention; in the present invention, it should also be noted that the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, an integrally formed connection, a mechanical connection, or an indirect connection through an intermediate connecting component, and the specific meaning of the terms in the present invention can be understood according to the specific circumstances.

[0049] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention, and any figure mark in the claims should not be regarded as limiting the claims involved.

[0050] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A culture device suitable for cell migration experiments, comprising a transparent cell culture plate (2) provided with a plurality of circular culture dishes (201), characterized in that: The bottom of the inner wall of the culture dish (201) is divided into a scratched area (201a) and two slanted bottom areas (201b); The scratched area (201a) is configured as a strip-shaped plane structure and is used to observe cell growth, and is arranged in the center, and the scratched areas (201a) of all culture dishes (201) are parallel to each other; The two inclined bottom areas (201b) are located on the left and right sides of the scratch area (201a) and are inclined downward relative to the scratch area (201a). The two inclined bottom areas (201b) form two receiving grooves for receiving the cell suspension (6).

2. A culture device suitable for cell migration experiments according to claim 1, characterized in that: It also comprises a bottom support (3) which can be tilted up and down at a certain angle, and the cell culture plate (2) is placed on the bottom support (3); when the cell suspension (6) is injected into the two inclined bottom areas (201b) in sequence, the bottom support (3) is adjusted to form two inclined angles of the cell culture plate (2), so that the two inclined bottom areas (201b) are respectively in a horizontal state.

3. A culture device suitable for cell migration experiments according to claim 2, characterized in that: Each culture dish (201) is provided with a transparent protection chamber (5) for protecting the scratched area (201a); the protection chamber (5) is configured as a protection cover structure; when in use, the protection chamber (5) covers and seals the scratched area (201a).

4. A culture device suitable for cell migration experiments according to claim 3, characterized in that: The scratch area (201a) has two long sides parallel to each other, and the two long sides of the scratch area (201a) are respectively connected to the two inclined bottom areas (201b). The scratch area (201a) is provided with two protection bin positioning grooves (201c) respectively located at two ends thereof. The protection bin (5) is a rectangular box-shaped structure as a whole and is parallel to the scratch area (201a). The two short side plates of the protection bin (5) are respectively inserted into the two protection bin positioning grooves (201c) so as to position the protection bin (5) at the position of the scratch area (201a).

5. A culture device suitable for cell migration experiments according to claim 2, characterized in that: The bottom of the bottom support (3) is connected to two groups of movable legs (4) distributed left and right so as to be rotatable and adjustable up and down, and each group of movable legs (4) has at least one movable leg. When the cell culture plate (2) is driven to tilt to a predetermined angle, one group of movable legs (4) is in an open state and supports the placement table, so that the bottom support (3) and the cell culture plate (2) are in a tilted state.

6. A culture device suitable for cell migration experiments according to claim 5, characterized in that: Each movable support foot (4) is provided with a support foot receiving groove (3a) for receiving the movable support foot (4), and the movable support foot (4) is connected to the support foot receiving groove (3a) in a manner of being rotatable up and down. The support foot receiving groove (3a) is arranged at the bottom of the bottom support (3), and when the bottom support (3) and the cell culture plate (2) are laid flat, all the movable support feet (4) are located in the support foot receiving groove (3a).

7. A culture device suitable for cell migration experiments according to claim 4, characterized in that: Both short side plates of the protection chamber (5) are connected with hanging ears (5a), and a pair of notches (202) for fixing the hanging ears (5a) are provided on the upper edge of the side wall of the culture dish (201), and the hanging ears (5a) are plugged into the notches (202).

8. A culture device suitable for cell migration experiments according to any one of claims 1 to 7, characterized in that: The bottom surface (204) of the cell culture plate (2) is provided with a plurality of scratch scale areas for measurement, which are matched one-to-one with the scratch areas (201a), each scratch scale area is provided with a plurality of scratch area scale lines (203), and each scratch scale area is directly opposite to the scratch area (201a) of the corresponding culture dish (201).

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