Nucleic acid extraction reagent strip

Abstract

The utility model discloses a nucleic acid extraction reagent strip which comprises a reagent strip body, one end of the reagent strip body is a fixed end, and the other end of the reagent strip body is a handheld end; a reagent hole, a plug hole, a piston hole, a waste liquid hole and a suction head hole are sequentially formed between the fixed end and the handheld end; a baffle is arranged below the fixed end and is used for blocking the optical sensor and judging whether the reagent strip is fixed in place or not; a bulge is arranged above the fixed end and is used for fixing the reagent strip; the handheld end is provided with a handle. The nucleic acid extraction reagent strip provided by the utility model can provide reagents and consumables required by nucleic acid extraction, and is finally used as a waste accommodating place, so that the treatment is convenient; the puncture sealing film and the waste liquid hole plug share one consumable which is a disposable consumable and does not need to be cleaned and replaced.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a nucleic acid extraction reagent strip. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. PCR reaction can specifically amplify extremely small amounts of DNA by millions of times, thereby greatly improving the ability to analyze and detect DNA molecules. Currently, the key factor leading to the failure of PCR reaction is the loss of control of purification quality, which leads to the loss or contamination of target DNA. Therefore, the purification of target DNA after replication is a key operation that directly affects the gene amplification reaction and directly affects the accuracy of disease detection results. The nucleic acid extraction box used in the PCR purification process is a disposable consumable used to obtain the purified material. It can accommodate various reaction reagents and provide reaction space. At the same time, it requires the sample to be anti-contamination during the target DNA replication and detection process.

[0003] Most of the reagent strips in the prior art only serve to carry reagents. For example, the utility model with application number CN201921953655.5 discloses a reagent strip, including a reagent strip body, from one end to the other end of the reagent strip body, there are arranged in sequence a sample hole with an open top and a closed bottom, a tip head sheath, a magnetic sleeve hole for storing a magnetic sleeve, a cleaning liquid storage hole, a reagent hole, a dilution hole, a reaction hole and a cleaning hole, and the magnetic sleeve is used to adsorb magnetic beads and stir.

[0004] Some reagent strips also have the function of carrying reagents and gun tips at the same time. For example, the invention patent with application number 201611029791.6 discloses a single-row nucleic acid extraction box suitable for nucleic acid diagnostic systems, including a box body, guide grooves are arranged on the left and right sides of the box body, an anti-drop plate is arranged on the front side of the box body, and the box body is provided with a purified material storage area, a reagent storage area, a gun tip storage area and a reaction area from left to right. Although the invention adds a gun tip storage area to facilitate the addition of reagents and the transfer of target reagents, it is still not reasonable for the treatment of discarded tips and waste liquids. Utility Model Content

[0005] In view of the deficiencies in the prior art, the purpose of the utility model is to provide a nucleic acid extraction reagent strip, which provides the reagents and consumables required for nucleic acid extraction and ultimately serves as a waste storage place for easy disposal; the puncture sealing film and the waste liquid hole plug share one consumable, which is a disposable consumable and does not require cleaning or replacement.

[0006] The technical solution adopted by the utility model to solve the technical problem is: a nucleic acid extraction reagent strip, the reagent strip comprising a reagent strip body, one end of the reagent strip body being a fixed end, and the other end being a handheld end; a reagent hole, a plug hole, a piston hole, a waste liquid hole and a suction tip hole are sequentially arranged between the fixed end and the handheld end; a baffle is arranged below the fixed end, the baffle is used to block an optical sensor, and is used to determine whether the reagent strip is fixed in place; a protrusion is arranged above the fixed end, the protrusion is used to fix the reagent strip; and a handle is arranged at the handheld end.

[0007] Furthermore, the number of the reagent wells is eight, and the reagent wells are used to store a variety of reagents required for nucleic acid extraction; each of the reagent wells is sealed with a film.

[0008] Furthermore, a disposable plug is placed in the plug hole, and the disposable plug is used to pierce the sealing film on the reagent hole and seal the waste liquid hole.

[0009] Furthermore, the number of the suction head holes is two, and the suction head holes are used to place the suction heads to be used or the used and discarded suction heads.

[0010] Furthermore, the reagent hole, plug hole, piston hole, waste liquid hole and suction head hole are all connected and fixed by a reinforcing plate; the reinforcing plate of the suction head hole close to the handheld end is a triangular reinforcing plate.

[0011] Furthermore, the plug hole and the piston hole include but are not limited to step holes.

[0012] The beneficial effects of the utility model are as follows: compared with the prior art, the nucleic acid extraction reagent strip provided by the utility model is provided with eight groups of reagent wells with sealing films (aluminum films), which contain a variety of reagents required for nucleic acid extraction (sample preservation solution, magnetic bead suspension, cleaning solution, lysis solution, eluent, inactivator, silicone oil); a plug hole is used to place a disposable plug for the sample adding arm group to obtain (similar to the method of taking a pipette tip), and then used to pierce the sealing film on the reagent well, and returned to its original position after use, and finally can be used to seal the waste liquid hole (also similar to the method of taking a pipette tip and hitting a pipette tip); a piston hole is placed in a microfluidic chip for nucleic acid amplification detection (similar to the method of taking a pipette tip and hitting a pipette tip), and is used to push liquid and seal; a waste liquid hole is used to collect waste liquid generated by all reaction processes, which can be sealed with a plug; two pipette tip holes are used to place pipette tips to be used, and used discarded pipette tips will also be pushed back here; one side is a handheld end, and the other side is stuck in a fixed end, and a baffle at the fixed end can block the optical sensor, which is used to judge whether the reagent strip is inserted in place.

[0013] The reagent strip provided by the utility model can provide the reagents and consumables required for nucleic acid extraction, and can ultimately serve as a waste storage place, which is convenient for disposal; the puncture sealing needle and the waste liquid hole plug share a consumable, which is a disposable consumable and does not need to be cleaned or replaced; it can prevent reagents from dripping onto the instrument or cross-reaction between reagents, which is safe and effective, and can also prevent waste liquid from being dumped due to improper operation when discarding. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the three-dimensional structure of the reagent strip provided by the utility model.

[0015] Figure 2 This is a schematic diagram of the three-dimensional structure of the reagent strip provided by the utility model.

[0016] Figure 3 This is a schematic diagram of the front structure of the reagent strip provided by the utility model.

[0017] Figure 4 This is a schematic diagram of the top structure of the reagent strip provided by the utility model.

[0018] Among them, 1-baffle; 2-protrusion; 3-fixed end; 4-reagent hole; 5-plug hole; 6-piston hole; 7-waste liquid hole; 8-reinforcement plate; 9-pipette tip hole; 10-handle; 11-handheld end; 12-triangular reinforcement plate; 13-sealing film. DETAILED DESCRIPTION

[0019] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively with reference to the relevant drawings. The drawings provide preferred embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the contents disclosed in the present invention.

[0020] Example

[0021] like Figures 1 to 4As shown, a nucleic acid extraction reagent strip, the reagent strip includes a reagent strip body, one end of the reagent strip body is a fixed end 3, and the other end is a handheld end 11; between the fixed end 3 and the handheld end 11, reagent holes 4, plug holes 5, piston holes 6, waste liquid holes 7 and suction tip holes 9 are sequentially arranged. The plug holes 5 and the piston holes 6 are both step holes. The number of the reagent holes 4 is eight, and the reagent holes 4 are used to store a variety of reagents needed for nucleic acid extraction, including sample preservation liquid, magnetic bead suspension, cleaning liquid, lysate, eluent, inactivator, and silicone oil; each of the reagent holes 4 is provided with a sealing film 13. The plug hole 5 is used to place a disposable plug, which is used to pierce the sealing film 13 on the reagent hole 4, and is returned to its original position after use, and can also be used to seal the waste liquid hole 7. The piston hole 6 is placed in a microfluidic chip for nucleic acid amplification detection for pushing liquid and sealing. There are two suction head holes 9 , and the suction head holes 9 are used to place suction heads to be used or used discarded suction heads.

[0022] A baffle 1 is provided below the fixed end 3, and the baffle 1 is used to block the optical sensor for determining whether the reagent strip is fixed in place; a protrusion 2 is provided above the fixed end 3, and the protrusion 2 is used to fix the reagent strip. The handheld end 11 is provided with a handle 10, and the handle 10 is provided with a plurality of convex strips to prevent slipping. The reagent hole 4, the plug hole 5, the piston hole 6, the waste liquid hole 7 and the suction tip hole 9 are all connected and fixed by a reinforcing plate 8, which can increase the overall stability of the reagent strip; the reinforcing plate of the suction tip hole 9 near the handheld end 11 is a triangular reinforcing plate 12, which can increase the stability of the handheld end 11.

[0023] The reagent strip provided by the utility model can provide the reagents and consumables required for nucleic acid extraction, and can ultimately serve as a waste storage place, which is convenient for disposal; the puncture sealing film 13 and the waste liquid hole 7 plug share a consumable, which is a disposable consumable and does not need to be cleaned or replaced.

[0024] The above implementation modes are only used to illustrate the present invention, but not to limit the present invention. Ordinary technicians in the relevant technical field can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention. The scope of patent protection of the present invention should be limited by the claims.

Claims

1. A nucleic acid extraction reagent strip, comprising a reagent strip body, characterized in that: One end of the reagent strip body is a fixed end, and the other end is a handheld end; a reagent hole, a plug hole, a piston hole, a waste liquid hole and a pipette tip hole are arranged in sequence between the fixed end and the handheld end; a baffle is arranged below the fixed end, and the baffle is used to block the optical sensor for judging whether the reagent strip is fixed in place; a protrusion is arranged above the fixed end, and the protrusion is used to fix the reagent strip; and a handle is arranged on the handheld end.

2. A nucleic acid extraction reagent strip as claimed in claim 1, characterized in that: There are eight reagent wells, which are used to store various reagents required for nucleic acid extraction; each reagent well is sealed with a film.

3. A nucleic acid extraction reagent strip as claimed in claim 1, characterized in that: A disposable plug is placed in the plug hole, and the disposable plug is used to pierce the sealing film on the reagent hole and seal the waste liquid hole.

4. A nucleic acid extraction reagent strip as claimed in claim 1, characterized in that: The number of the suction head holes is two, and the suction head holes are used to place the suction heads to be used or the used discarded suction heads.

5. A nucleic acid extraction reagent strip as claimed in claim 1, characterized in that: The reagent hole, plug hole, piston hole, waste liquid hole and suction head hole are all connected and fixed by a reinforcing plate; the reinforcing plate at the suction head hole close to the handheld end is a triangular reinforcing plate.

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