Automatic cleaning device for reagent sample adding box

Abstract

The utility model provides an automatic cleaning device of a reagent sample adding box, which relates to the field of cleaning devices.The automatic cleaning device of the reagent sample adding box comprises a placing frame and a plurality of water passing columns, and a movable device used for adjusting the positions of the water passing columns and a water injection device used for injecting water into the water passing columns are installed on the placing frame. Multiple rows of cleaning brushes and multiple rows of water spraying pipes are fixedly connected to the periphery of the outer surface of the water passing column, inner cavities of the water spraying pipes communicate with an inner cavity of the water passing column, the multiple rows of cleaning brushes and the multiple rows of water spraying pipes are arranged in a staggered mode, and the multiple cleaning brushes in one row and the multiple cleaning brushes in the adjacent row are arranged in a staggered mode; according to the automatic cleaning device of the reagent sample adding box, the direct contact area between the inner wall of the reagent groove and the cleaning brush and the direct contact area between the inner wall of the reagent groove and the water spraying pipes are increased, and cleaning is more sufficient.

Description

Technical Field

[0001] The utility model relates to the field of cleaning devices, in particular to an automatic cleaning device for a reagent adding box. Background Art

[0002] Pyrosequencing is a sequencing technology based on an enzymatic cascade reaction, which determines the DNA sequence by detecting the release of pyrophosphate. In this process, a series of specific reagents are required, including enzymes, substrates, buffers, etc. The amount of these reagents added needs to be precisely controlled to ensure the smooth progress of the sequencing reaction.

[0003] The reagent loading box for pyrophosphate sequencing is a reagent storage and adding device specially designed for pyrophosphate sequencing experiments. The loading box not only facilitates the storage and management of reagents, but also optimizes the reagent adding steps in the sequencing process, thereby improving the accuracy and efficiency of sequencing.

[0004] The design of the reagent loading box takes into account the special needs of pyrophosphate sequencing. It usually has multiple independent reagent slots for storing different reagents. Each reagent slot is clearly marked for easy identification and operation by experimenters. After the use of the reagent loading box of pyrophosphate sequencing is completed, in order to facilitate the next use, the reagent loading box needs to be cleaned. The existing cleaning device generally directly injects pure water into the reagent slot of the reagent kit through a water pipe, and shakes the cleaning liquid to continuously contact the inner wall of the reagent kit. In this cleaning method, the contact force between the tilting liquid and the inner wall of the reagent kit is small, which is prone to insufficient cleaning. If it is directly cleaned with an external brush, the operation is relatively troublesome.

[0005] Therefore, it is necessary to provide a new automatic cleaning device for a reagent loading box to solve the above technical problems. Utility Model Content

[0006] In order to solve the above technical problems, the utility model provides an automatic cleaning device for a reagent adding box.

[0007] The automatic cleaning device of the reagent loading box provided by the utility model comprises a placement rack and a plurality of water columns, wherein a movable device for adjusting the positions of the plurality of water columns and a water injection device for injecting water into the plurality of water columns are installed on the placement rack, a plurality of rows of cleaning brushes and a plurality of rows of water spray pipes are fixedly connected to the outer surfaces of the water columns on all sides, the inner cavities of the water spray pipes are connected with the inner cavities of the water columns, the plurality of rows of cleaning brushes and the plurality of rows of water spray pipes are staggered, a plurality of cleaning brushes in a row are staggered with a plurality of cleaning brushes in an adjacent row, a plurality of water spray pipes in a row are staggered with a plurality of water spray pipes in an adjacent row, and the apertures of the water spray pipes gradually decrease in a direction away from the water injection device.

[0008] Preferably, the movable device includes a moving frame, a threaded rod and a first motor, the end of the moving frame is fixedly mounted on the placement frame, the threaded rod is movably connected to the moving frame, a transmission frame is threadedly connected to the threaded rod, a lifting member is installed at the end of the transmission frame, the first motor is installed at one end of the moving frame, and the output end of the first motor is fixedly connected to the end of the threaded rod.

[0009] Preferably, the lifting member includes an electric cylinder, a connecting rod and a mounting plate, the electric cylinder is fixedly mounted on the transmission frame, the telescopic end of the electric cylinder is fixedly connected to the connecting rod, the bottom end of the connecting rod is fixedly connected to the mounting plate, and the mounting plate is connected to the water injection device.

[0010] Preferably, the water injection device includes a water passing part, a water passing pipe and a water diversion plate, the water passing part is installed on a placement rack, the water passing pipe is movably inserted into a through hole opened in the installation plate, the inner cavity of the water passing pipe is connected to the water passing part, the bottom of the water passing pipe is fixedly connected to the water diversion plate, and the bottom of the water diversion plate is fixedly connected to multiple water passing columns.

[0011] Preferably, the water-passing component includes a water tank, a water pump and a hose, the water tank and the water pump are both installed on a placement rack, the inner cavity of the water tank is connected to the input end of the water pump, the hose is connected to the output end of the water pump, and the end of the hose away from the water pump is fixedly connected to the water pipe.

[0012] Preferably, a reciprocating motion device is installed on the mounting plate to drive the water pipe to move up and down.

[0013] Preferably, the reciprocating motion device includes a driving member, a fixing frame and a spring, the fixing frame is fixedly mounted on the water pipe, the two ends of the spring are respectively fixedly connected to the bottom of the fixing frame and the top of the mounting plate, and the driving member is mounted on the mounting plate.

[0014] Preferably, the driving member includes a second motor and an eccentric wheel, the second motor is fixedly mounted on the mounting plate, the output end of the second motor is fixedly connected to the eccentric wheel, and the eccentric wheel is located below one end of the fixing frame.

[0015] Preferably, a cleaning bin is placed on the placement rack, and a placement plate is installed inside the cleaning bin.

[0016] Preferably, a photosensitive sensor module is installed on the side wall of the cleaning chamber, a control room is provided on one side of the cleaning chamber, and a relay group module, a PLC controller module and a GPRS communication module are installed in the control room.

[0017] Compared with the related art, the automatic cleaning device of the reagent sample adding box provided by the utility model has the following beneficial effects:

[0018] A plurality of water columns are driven to move to the top of the reagent kit by a movable device, and the plurality of water columns respectively correspond to a plurality of independent reagent slots on the reagent kit. Then, the plurality of water columns are driven downward by the movable device, so that the plurality of water columns are respectively moved to the inner sides of the plurality of reagent slots, and the cleaning liquid is sprayed out from the water spray pipe by the water injection device. Since the aperture of the water spray pipe gradually decreases in the direction away from the water injection device, the force of the liquid spraying is increased. In addition, a plurality of rows of cleaning brushes and a plurality of rows of water spray pipes are staggered, and a plurality of cleaning brushes in a row of cleaning brushes are staggered with a plurality of cleaning brushes in an adjacent row of cleaning brushes, and a plurality of water spray pipes in a row of water spray pipes are staggered with a plurality of water spray pipes in an adjacent row of water spray pipes, thereby increasing the area of ​​direct contact between the inner wall of the reagent slot and the cleaning brush and the water spray pipe, so that the cleaning is more thorough. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the structure of the automatic cleaning device of the reagent loading box provided by the utility model;

[0020] Figure 2 for Figure 1 The schematic diagram of the local structure shown;

[0021] Figure 3 for Figure 2 The structural schematic diagram of A shown;

[0022] Figure 4 for Figure 2 The schematic diagram of the structure of the water column, the water spray pipe and the cleaning brush shown;

[0023] Figure 5 for Figure 4 A schematic structural diagram of a partial cross section of the structure shown;

[0024] Figure 6 A schematic diagram of the structure of the test kit cleaned by the utility model;

[0025] Figure 7 for Figure 6 A schematic diagram of the structure of the kit in a top view;

[0026] Figure 8 for Figure 6 Schematic diagram of the structure of the reagent kit after cleaning and placing it in a beaker.

[0027] Numbers in the figure: 1. Placement rack; 2. Cleaning chamber; 3. Water column; 4. Water spray pipe; 5. Cleaning brush; 6. Mobile rack; 7. Threaded rod; 8. First motor; 9. Transmission rack; 10. Electric cylinder; 11. Connecting rod; 12. Mounting plate; 13. Water pipe; 14. Water guide plate; 15. Fixed rack; 16. Spring; 17. Second motor; 18. Eccentric wheel; 19. Water tank; 20. Water pump; 21. Hose; 22. Placement plate; 23. Photosensor module; 24. Relay group module; 25. PLC controller module; 26. GPRS communication module; 27. Reagent box. DETAILED DESCRIPTION

[0028] The utility model is further described below in conjunction with the accompanying drawings and implementation modes.

[0029] Please refer to Figure 1 , Figure 2 , Figure 3 and Figure 4 ,in, Figure 1 A schematic diagram of the structure of the automatic cleaning device of the reagent loading box provided by the utility model; Figure 2 for Figure 1 The schematic diagram of the local structure shown; Figure 3 for Figure 2 The structural schematic diagram of A shown; Figure 4 for Figure 2 The schematic diagram of the structure of the water column, the water spray pipe and the cleaning brush shown; Figure 5 for Figure 4 A schematic structural diagram of a partial cross section of the structure shown;

[0030] Figure 6 A schematic diagram of the structure of the test kit cleaned by the utility model; Figure 7 for Figure 6 A schematic diagram of the structure of the kit in a top view; Figure 8 for Figure 6 Schematic diagram of the structure of the reagent kit after cleaning and placing it in a beaker.

[0031] In the specific implementation process, Figure 1-Figure 5As shown, a placement rack 1 and multiple water columns 3 are installed on the placement rack 1. A movable device for adjusting the positions of the multiple water columns 3 and a water injection device for passing water into the multiple water columns 3 are installed. The size of the water columns 3 can be made according to the size of each reagent tank in the reagent kit. Multiple rows of cleaning brushes 5 and multiple rows of water spray pipes 4 are fixedly connected to the outer surface of the water columns 3. The inner cavity of the water spray pipe 4 is connected to the inner cavity of the water columns 3. The multiple rows of cleaning brushes 5 and the multiple rows of water spray pipes 4 are staggered. Multiple cleaning brushes 5 in a row of cleaning brushes 5 are staggered with multiple cleaning brushes 5 in an adjacent row of cleaning brushes 5. Multiple water spray pipes 4 in a row of water spray pipes 4 are staggered with multiple water spray pipes 4 in an adjacent row of water spray pipes 4. The aperture of the water spray pipe 4 gradually decreases in the direction away from the water injection device, and the multiple water columns 3 are driven by the movable device. Move to the top of the reagent box, and multiple water columns 3 respectively correspond to multiple independent reagent tanks on the reagent box. Then, the multiple water columns 3 are driven downward by the movable device, so that the multiple water columns 3 are respectively moved to the inner sides of the multiple reagent tanks, and the cleaning liquid is sprayed from the water spray pipe 4 through the water injection device. Since the aperture of the water spray pipe 4 gradually decreases in the direction away from the water injection device, the force of liquid spraying is increased. In addition, multiple rows of cleaning brushes 5 and multiple rows of water spray pipes 4 are staggered, and multiple cleaning brushes 5 in a row of cleaning brushes 5 are staggered with multiple cleaning brushes 5 in an adjacent row of cleaning brushes 5, and multiple water spray pipes 4 in a row of water spray pipes 4 are staggered with multiple water spray pipes 4 in an adjacent row of water spray pipes 4, thereby increasing the area of ​​direct contact between the inner wall of the reagent tank and the cleaning brush 5 and the water spray pipe 4, so that cleaning is more sufficient;

[0032] The movable device includes a moving frame 6, a threaded rod 7 and a first motor 8, the end of the moving frame 6 is fixedly mounted on the placement frame 1, the threaded rod 7 is movably connected to the moving frame 6, a transmission frame 9 is threadedly connected to the threaded rod 7, and a lifting member is installed at the end of the transmission frame 9, the first motor 8 is installed at one end of the moving frame 6, and the output end of the first motor 8 is fixedly connected to the end of the threaded rod 7, the lifting member includes an electric cylinder 10, a connecting rod 11 and a mounting plate 12, the electric cylinder 10 is fixedly mounted on the transmission frame 9, the telescopic end of the electric cylinder 10 is fixedly connected to the connecting rod 11, the bottom end of the connecting rod 11 is fixedly connected to the mounting plate 12, the mounting plate 12 is connected to the water injection device, the threaded rod 7 is driven to rotate by the first motor 8, so that the transmission frame 9 drives the multiple water columns 3 to move to the top of the reagent box, and the multiple water columns 3 correspond to the multiple independent reagent tanks on the reagent box respectively, then, the mounting plate 12 is driven downward by the electric cylinder 10, so that the multiple water columns 3 are respectively moved to the inner side of the reagent tank;

[0033] The water injection device includes a water-passing member, a water-passing pipe 13 and a water-introducing plate 14, wherein the water-passing member is installed on the placement rack 1, the water-passing pipe 13 is movably inserted into the through hole provided in the installation plate 12, the inner cavity of the water-passing pipe 13 is connected to the water-passing member, the bottom of the water-passing pipe 13 is fixedly connected to the water-introducing plate 14, the bottom of the water-introducing plate 14 is fixedly connected to a plurality of water-passing columns 3, the water-passing member includes a water tank 19, a water pump 20 and a hose 21, the water tank 19 and the water pump 20 are both installed on the placement rack 1, the inner cavity of the water tank 19 is connected to the input end of the water pump 20, the hose 21 is connected to the output end of the water pump 20, the end of the hose 21 away from the water pump 20 is fixedly connected to the water-passing pipe 13, the water pump 20 draws pure water into the hose 21 through the water tank 19, and the pure water is sprayed out from the water spray pipe 4 after passing through the water-passing pipe 13, the water-introducing plate 14 and the water-passing columns 3;

[0034] A reciprocating motion device for driving the water pipe 13 to move up and down is installed on the mounting plate 12, and the reciprocating motion device includes a driving member, a fixing frame 15 and a spring 16. The fixing frame 15 is fixedly sleeved on the water pipe 13, and the two ends of the spring 16 are respectively fixedly connected to the bottom of the fixing frame 15 and the top of the mounting plate 12. The driving member is installed on the mounting plate 12, and the driving member includes a second motor 17 and an eccentric wheel 18. The second motor 17 is fixedly installed on the mounting plate 12, and the output end of the second motor 17 is fixedly connected to the eccentric wheel 18, and the eccentric wheel 18 is located below one end of the fixing frame 15;

[0035] A cleaning chamber 2 is placed on the placement rack 1, and a placement plate 22 is installed inside the cleaning chamber 2;

[0036] A photosensitive sensor module 23 is installed on the side wall of the cleaning chamber 2, and a control room is set on one side of the cleaning chamber 2. The control room is equipped with a relay group module 24, a PLC controller module 25 and a GPRS communication module 26.

[0037] The working principle provided by the utility model is as follows: when the device is used, the reagent box is placed on the placement plate 22, and the first motor 8 drives the threaded rod 7 to rotate, so that the transmission frame 9 drives the multiple water columns 3 to move to the top of the reagent box, and the multiple water columns 3 respectively correspond to the multiple independent reagent tanks on the reagent box. Then, the electric cylinder 10 drives the mounting plate 12 to move downward, so that the multiple water columns 3 are respectively moved to the inner side of the reagent tank, and the water pump 20 draws pure water into the hose 21 through the water tank 19. The pure water is sprayed out from the water pipe 4 after passing through the water pipe 13, the water guide plate 14 and the water column 3. Since the aperture of the water pipe 4 gradually decreases in the direction away from the water injection device, the force of the liquid spraying is increased, and the second motor 17 drives As the eccentric wheel 18 rotates, under the action of the spring 16, the eccentric wheel 18 continuously hits the fixed frame 15, so that the water column 3 drives the water spray pipe 4 and the cleaning brush 5 thereon to move up and down, so as to fully clean the inner wall of the reagent tank in the reagent kit. In addition, through the photosensitive sensor module 23, the photosensitive sensor module 23 detects the reagent kit to facilitate the observation of whether the reagent kit is fully cleaned. The photosensitive sensor module 23 transmits the received signal to the PLC controller module 25, and the PLC controller module 25 controls the switching status of the water pump 20 through the relay group module 24, and the PLC controller module 25 is connected to the GPRS communication module 26, and the signal is transmitted to the cloud through the GPRS communication module 26.

[0038] The circuits and controls involved in the present invention are all prior art and will not be described in detail here.

[0039] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An automatic cleaning device for a reagent loading box, characterized in that: The invention comprises a placing rack (1) and a plurality of water-passing columns (3). The placing rack (1) is provided with a movable device for adjusting the positions of the plurality of water-passing columns (3) and a water injection device for injecting water into the plurality of water-passing columns (3). The outer surfaces of the water-passing columns (3) are all fixedly connected with a plurality of rows of cleaning brushes (5) and a plurality of rows of water spray pipes (4). The inner cavities of the water spray pipes (4) are connected with the inner cavities of the water-passing columns (3). The plurality of rows of cleaning brushes (5) and the plurality of rows of water spray pipes (4) are arranged in a staggered manner. The plurality of cleaning brushes (5) in a row of cleaning brushes (5) are arranged in a staggered manner with the plurality of cleaning brushes (5) in an adjacent row of cleaning brushes (5). The plurality of water spray pipes (4) in a row of water spray pipes (4) are arranged in a staggered manner with the plurality of water spray pipes (4) in an adjacent row of water spray pipes (4). The apertures of the water spray pipes (4) gradually decrease in a direction away from the water injection device.

2. The automatic cleaning device for the reagent loading box according to claim 1, characterized in that: The movable device comprises a movable frame (6), a threaded rod (7) and a first motor (8); the end of the movable frame (6) is fixedly mounted on the placement frame (1); the threaded rod (7) is movably connected to the movable frame (6); a transmission frame (9) is threadedly connected to the threaded rod (7); a lifting member is mounted on the end of the transmission frame (9); the first motor (8) is mounted on one end of the movable frame (6); and the output end of the first motor (8) is fixedly connected to the end of the threaded rod (7).

3. The automatic cleaning device for the reagent loading box according to claim 2, characterized in that: The lifting member comprises an electric cylinder (10), a connecting rod (11) and a mounting plate (12); the electric cylinder (10) is fixedly mounted on a transmission frame (9); the telescopic end of the electric cylinder (10) is fixedly connected to the connecting rod (11); the bottom end of the connecting rod (11) is fixedly connected to the mounting plate (12); and the mounting plate (12) is connected to a water injection device.

4. The automatic cleaning device for the reagent loading box according to claim 3, characterized in that: The water injection device comprises a water-passing member, a water-passing pipe (13) and a water-introducing plate (14); the water-passing member is mounted on the placement frame (1); the water-passing pipe (13) is movably inserted into a through hole provided in the mounting plate (12); the inner cavity of the water-passing pipe (13) is connected to the water-passing member; the bottom of the water-passing pipe (13) is fixedly connected to the water-introducing plate (14); and the bottom of the water-introducing plate (14) is fixedly connected to a plurality of the water-passing columns (3).

5. The automatic cleaning device for the reagent loading box according to claim 4, characterized in that: The water-passing member comprises a water tank (19), a water pump (20) and a hose (21); the water tank (19) and the water pump (20) are both mounted on a placement rack (1); the inner cavity of the water tank (19) is connected to the input end of the water pump (20); the hose (21) is connected to the output end of the water pump (20); and one end of the hose (21) away from the water pump (20) is fixedly connected to the water-passing pipe (13).

6. The automatic cleaning device for the reagent loading box according to claim 5, characterized in that: A reciprocating motion device for driving the water pipe (13) to move up and down is installed on the installation plate (12).

7. The automatic cleaning device for the reagent loading box according to claim 6, characterized in that: The reciprocating motion device comprises a driving member, a fixing frame (15) and a spring (16); the fixing frame (15) is fixedly sleeved on the water pipe (13); two ends of the spring (16) are respectively fixedly connected to the bottom of the fixing frame (15) and the top of the mounting plate (12); and the driving member is mounted on the mounting plate (12).

8. The automatic cleaning device for the reagent loading box according to claim 7, characterized in that: The driving member comprises a second motor (17) and an eccentric wheel (18); the second motor (17) is fixedly mounted on the mounting plate (12); the output end of the second motor (17) is fixedly connected to the eccentric wheel (18); and the eccentric wheel (18) is located below one end of the fixing frame (15).

9. The automatic cleaning device for the reagent loading box according to claim 6, characterized in that: A cleaning bin (2) is placed on the placement rack (1), and a placement plate (22) is installed inside the cleaning bin (2).

10. The automatic cleaning device for the reagent loading box according to claim 9, characterized in that: A photosensitive sensor module (23) is installed on the side wall of the cleaning chamber (2), and a control room is provided on one side of the cleaning chamber (2), in which a relay group module (24), a PLC controller module (25) and a GPRS communication module (26) are installed.

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