Cell culture bioreactor

Abstract

The utility model discloses a cell culture bioreactor, which belongs to the technical field of cell culture and comprises a box body, a water tank is connected above the box body, and a water injection component is arranged above the water tank; the device is provided with the fixing assembly, the jacking block is moved upwards, the jacking block moves to drive the connecting rod to move, the connecting rod moves to drive the first spring to stretch, then a culture dish is placed on the placing plate, the rotating nut is rotated to rotate and move along the fixing screw rod, and after the rotating nut is far away from the moving block, the second spring loses the external force to restore the original appearance and drives the moving block to move. After the pressing block moves to the end position of the culture dish, the jacking block is loosened, the first spring loses the external force to restore the original appearance, the connecting rod is driven to move downwards, the connecting rod moves downwards to drive the jacking block to move downwards, and the jacking block moves downwards to drive the pressing block to move downwards to press the culture dish; the placement stability of the culture dish is ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and in particular relates to a cell culture bioreactor. Background Art

[0002] Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. Whether it is for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an indispensable process. Cell culture itself is the large-scale cloning of cells.

[0003] Chinese patent application No. 201922324480.8 discloses an immune cell culture bioreactor, including a box, the inner wall of the box is fixedly connected with a partition, the bottom of the partition and the top of the box inner cavity are fixedly connected with a water spray plate, the left side of the box inner cavity is fixedly connected with a heating plate, the right side of the box inner cavity is fixedly connected with a temperature and humidity sensor, and the back of the box inner cavity is fixedly connected with a lighting lamp. The utility model achieves the purpose of good use effect through the cooperation of a heating plate, a temperature and humidity sensor, a lighting lamp, a water tank, a water pipe and a solenoid valve, can adjust the temperature and humidity inside the immune cell culture bioreactor according to the use requirements, improves the user's experience of the immune cell culture bioreactor, meets the needs of the current market, improves the practicality and usability of the immune cell culture bioreactor, and solves the problem of poor use effect of the previous immune cell culture bioreactor.

[0004] In the above-mentioned patent, the culture dish is directly placed on the placement plate, and the culture dish is easily displaced on the placement plate due to the subsequent force, which affects the stability of the placement of the culture dish and further affects the cell culture effect; in addition, water is directly injected into the water tank. When impurities remain in the injected water, the impurities are sprayed onto the cells in the culture dish, affecting the cell culture effect. Utility Model Content

[0005] In order to solve the problems raised in the above background technology, the utility model provides a cell culture bioreactor, which has the characteristics of tight fixation and filtering and removing impurities.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture bioreactor, comprising a box body, a water tank is connected to the top of the box body, a water injection assembly is arranged above the water tank, an adjustment mechanism is arranged between the water tank and the box body, a placement plate is connected to the inside of the box body, a culture dish is arranged inside the placement plate, a fixing assembly is arranged between the culture dish and the placement plate, a box door is connected to one side of the water tank, and a movable support mechanism is connected to the bottom of the box body.

[0007] Preferably, the fixing assembly includes a fixed cylinder, a first spring, a connecting rod, a top block, a clamping block and a movable part. The interior of the placement plate and both sides of the culture dish are connected with a fixed cylinder, a connecting rod is inserted into the interior of the fixed cylinder, a first spring is connected between the fixed cylinder and the connecting rod, an end of the connecting rod away from the fixed cylinder is connected to a top block, a clamping block is provided on the side of the top block close to the culture dish, and a movable part is provided between the top block and the clamping block.

[0008] Preferably, both sides of the connecting rod at one end close to the fixed cylinder are connected to limiting sliders, and limiting sliding grooves are provided on the inner side wall of the fixed cylinder at positions corresponding to the limiting sliders.

[0009] Preferably, the moving part includes an end block, a fixed screw, a rotating nut, a moving block, a second spring and an insertion rod, the end of the clamping block close to the top block is connected to the insertion rod, the end of the insertion rod close to the top block is connected to the moving block, both ends above the top block are connected to the end blocks, a fixed screw is connected between the two end blocks, a through hole is provided on the moving block at a position corresponding to the fixed screw, a second spring is connected between the moving block and the end block and located at the periphery of the fixed screw, and a rotating nut is connected on the outer wall of the fixed screw and located on the side of the moving block away from the second spring.

[0010] Preferably, the water injection assembly includes an end plate, a filter screen plate, a connecting frame and a fixing rod. An end plate is arranged above the water tank, and both ends of the end plate close to the water tank are connected to the fixing rods, and the end of the fixing rod away from the end plate is connected to the connecting frame, and the filter screen plate is connected to the inside of the connecting frame.

[0011] Preferably, a fixing frame is connected to the inner wall of the water tank and located below the connecting frame, positioning protrusions are connected to both ends of one side of the connecting frame close to the fixing frame, and a positioning groove is provided inside the fixing frame and at the position corresponding to the positioning protrusion.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model sets a fixed component, moves the top block upward, the movement of the top block drives the connecting rod to move, the movement of the connecting rod drives the first spring to stretch, then the culture dish is placed on the placement plate, and the rotating nut is turned to make it rotate and move along the fixed screw. After the rotating nut is away from the moving block, the second spring loses the external force and restores to its original state, drives the moving block to move, the movement of the moving block drives the insertion rod to move, the movement of the insertion rod drives the clamping block to move, when the clamping block moves to the end position of the culture dish, releases the top block, the first spring loses the external force and restores to its original state, drives the connecting rod to move downward, the connecting rod moves downward and drives the top block to move downward, the top block moves downward and drives the clamping block to move downward to perform a clamping operation on the culture dish, thereby ensuring the stability of the placement of the culture dish.

[0014] 2. The utility model is provided with a water injection assembly, and the end plate is installed on the top of the water tank. Under the action of the fixing rod, the connecting frame is driven to move to the top of the fixing frame, so that the filter screen plate can be installed inside the water tank. The operator then opens the end cover on the top of the water injection pipe on the end plate, and injects cell culture water into the water tank through the water injection pipe. The injected water can be filtered by the filter screen plate to ensure the cleanliness of the water entering the inside of the regulating mechanism, and then the cleanliness of the cell culture water sprayed into the inside of the box can be ensured, thereby ensuring the cell culture effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of the utility model;

[0016] Figure 2 It is a cross-sectional view of the utility model;

[0017] Figure 3 It is a cross-sectional view of the connection state of the placement plate and the culture dish of the utility model;

[0018] Figure 4 For this utility model Figure 3 The enlarged view of point A in the middle;

[0019] Figure 5 It is a cross-sectional view of the connection state between the top block and the pressing block of the utility model;

[0020] Figure 6 It is a cross-sectional view of the water tank of the utility model;

[0021] Figure 7 For this utility model Figure 6 Enlarged view of point B in the middle.

[0022] In the figure: 1. water tank; 2. box body; 3. box door; 4. movable support mechanism; 5. culture dish; 6. placement plate; 7. adjustment mechanism; 8. fixing assembly; 81. fixing cylinder; 82. first spring; 83. connecting rod; 84. top block; 85. pressing block; 86. moving part; 861. end block; 862. fixing screw; 863. rotating nut; 864. moving block; 865. second spring; 866. plug rod; 9. water injection assembly; 91. end plate; 92. filter screen plate; 93. connecting frame; 94. fixing rod; 95. fixing frame. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Example 1

[0025] See also Figure 1-7 The utility model provides the following technical solutions: a cell culture bioreactor, comprising a box body 2, a water tank 1 is connected to the top of the box body 2, a water injection assembly 9 is arranged above the water tank 1, an adjustment mechanism 7 is arranged between the water tank 1 and the box body 2, a placement plate 6 is connected to the inside of the box body 2, a culture dish 5 is arranged inside the placement plate 6, a fixing assembly 8 is arranged between the culture dish 5 and the placement plate 6, a box door 3 is connected to one side of the water tank 1, and a movable support mechanism 4 is connected to the bottom of the box body 2.

[0026] Specifically, the fixing assembly 8 includes a fixing cylinder 81, a first spring 82, a connecting rod 83, a top block 84, a pressing block 85 and a moving member 86. The inside of the placement plate 6 and both sides of the culture dish 5 are connected with the fixing cylinder 81, the inside of the fixing cylinder 81 is plugged with the connecting rod 83, the first spring 82 is connected between the fixing cylinder 81 and the connecting rod 83, the end of the connecting rod 83 away from the fixing cylinder 81 is connected with the top block 84, the side of the top block 84 close to the culture dish 5 is provided with a pressing block 85, and the moving member 86 is provided between the top block 84 and the pressing block 85.

[0027] By adopting the above technical solution, the top block 84 is first moved up, and the movement of the top block 84 drives the connecting rod 83 to move inside the fixed tube 81, and the movement of the connecting rod 83 drives the first spring 82 to stretch, and then the culture dish 5 is placed on the placement plate 6, and then the moving member 86 is used to drive the clamping block 85 to move. When the clamping block 85 moves to the end position of the culture dish 5, the top block 84 is released, and the first spring 82 loses the external force and returns to its original state, driving the connecting rod 83 to move downward, and the connecting rod 83 moves downward to drive the top block 84 to move downward, and the top block 84 moves downward to drive the clamping block 85 to move downward to perform a clamping operation on the culture dish 5, thereby ensuring the stability of the placement of the culture dish 5.

[0028] Specifically, both sides of the end of the connecting rod 83 close to the fixed cylinder 81 are connected to the limit sliders, and the inner wall of the fixed cylinder 81 is provided with a limit slide groove at the position corresponding to the limit slider.

[0029] By adopting the above technical solution, the movement of the connecting rod 83 drives the limit slider to move inside the limit slide groove. When the limit slider moves to fit the side wall of the limit slide groove, the limit slider is blocked and stops moving, thereby preventing the connecting rod 83 from continuing to move and preventing the connecting rod 83 from detaching from the fixed tube 81.

[0030] Specifically, the moving member 86 includes an end block 861, a fixed screw 862, a rotating nut 863, a moving block 864, a second spring 865 and an insertion rod 866. The end of the pressing block 85 close to the top block 84 is connected to the insertion rod 866, and the end of the insertion rod 866 close to the top block 84 is connected to the moving block 864. The upper ends of the top block 84 are connected to the end blocks 861, and the fixed screw 862 is connected between the two end blocks 861. A through hole is provided on the moving block 864 and at a position corresponding to the fixed screw 862. The second spring 865 is connected between the moving block 864 and the end block 861 and at the periphery of the fixed screw 862. The rotating nut 863 is connected to the outer wall of the fixed screw 862 and at the side of the moving block 864 away from the second spring 865.

[0031] By adopting the above technical solution, the rotating nut 863 is turned to rotate and move along the fixed screw 862. After the rotating nut 863 moves away from the moving block 864, the second spring 865 loses the external force and returns to its original state, driving the moving block 864 to move. The movement of the moving block 864 drives the insertion rod 866 to move. The movement of the insertion rod 866 drives the clamping block 85 to move, which makes it easy to adjust the position of the clamping block 85, and then makes it easy to use the clamping block 85 to clamp and fix culture dishes 5 of different sizes.

[0032] When the present embodiment is used, the movable support mechanism 4 is used to move the box body 2 to the cell culture site, and then the box door 3 is opened, and then the top block 84 is moved upward, and the top block 84 moves to drive the connecting rod 83 to move inside the fixed cylinder 81, and the connecting rod 83 moves to drive the first spring 82 to stretch, and then the culture dish 5 is placed on the placement plate 6, and the rotating nut 863 is turned to rotate and move along the fixed screw 862. After the rotating nut 863 moves away from the moving block 864, the second spring 865 loses the external force and returns to its original state, driving the moving block 864 to move, and the moving block 864 moves to drive the insertion rod 866 to move, and the insertion rod 866 moves. The pressing block 85 is driven to move. When the pressing block 85 moves to the end position of the culture dish 5, the top block 84 is released, and the first spring 82 loses the external force and returns to its original state, driving the connecting rod 83 to move downward. The connecting rod 83 moves downward to drive the top block 84 to move downward. The top block 84 moves downward to drive the pressing block 85 to move downward to press the culture dish 5 to ensure the stability of the placement of the culture dish 5. Then, the cell culture material is placed in the culture dish 5, and then the box door 3 is closed. The temperature and humidity inside the box body 2 are adjusted by the combined action of the adjustment mechanism 7 and the water tank 1 to ensure that the temperature and humidity inside the box body 2 meet the cell culture requirements.

[0033] Example 2

[0034] The present embodiment is different from the embodiment 1 in that: the water injection assembly 9 includes an end plate 91, a filter screen plate 92, a connection frame 93 and a fixing rod 94. The end plate 91 is arranged above the water tank 1. The two ends of the end plate 91 close to the water tank 1 are connected to the fixing rod 94. The end of the fixing rod 94 away from the end plate 91 is connected to the connection frame 93. The filter screen plate 92 is connected inside the connection frame 93.

[0035] Specifically, a fixing frame 95 is connected to the inner wall of the water tank 1 and is located below the connecting frame 93. Positioning protrusions are connected to both ends of the connecting frame 93 on one side close to the fixing frame 95. Positioning grooves are provided inside the fixing frame 95 at positions corresponding to the positioning protrusions.

[0036] By adopting the above technical solution, after the connecting frame 93 is placed inside the water tank 1, the positioning protrusion is inserted into the positioning groove, and the fixing frame 95 can support the connecting frame 93. At the same time, under the positioning operation of the positioning protrusion and the positioning groove, the connecting frame 93 and the fixing frame 95 can be positioned to ensure the stability of the connection between the connecting frame 93 and the fixing frame 95.

[0037] When this embodiment is used, the end plate 91 is installed on the top of the water tank 1, and the connecting frame 93 is moved to the top of the fixing frame 95 under the action of the fixing rod 94, so that the filter screen plate 92 can be installed inside the water tank 1. The operator then opens the end cover on the top of the water injection pipe on the end plate 91, and injects cell culture water into the water tank 1 through the water injection pipe. The filter screen plate 92 can be used to filter the injected water to ensure the cleanliness of the water entering the regulating mechanism 7, and then the cleanliness of the cell culture water sprayed into the box body 2 can be ensured, thereby ensuring the cell culture effect.

[0038] The structure and principle of the mobile support mechanism 4 composed of vertical plates, rollers, vertical grooves, electric telescopic rods, connecting rods, cross plates, limit blocks, reinforcing plates, vertical rods, and fixed plates in the utility model have been disclosed in an immune cell culture bioreactor disclosed in Chinese patent application No. 201922324480.8. Its working principle is: both sides of the bottom of the box body 2 are fixedly connected with vertical plates, the bottom of the vertical plates are movably connected with rollers through a rotating rod, a vertical groove is provided on the opposite side of the vertical plates, the bottom of the box body 2 is fixedly connected with an electric telescopic rod, the output end of the electric telescopic rod is movably connected to the connecting rod through a rotating rod, and the end of the connecting rod away from the output end of the electric telescopic rod is connected by a rotating rod. The rotating rod is movably connected to a horizontal plate, and both sides of the horizontal plate are fixedly connected to limit blocks. The surface of the limit block is slidably connected to the inner wall of the vertical groove, and the bottom of the opposite side of the vertical plate is fixedly connected to a reinforcing plate, and the bottom of the horizontal plate is fixedly connected to a vertical rod, and the bottom of the vertical rod penetrates to the outside of the reinforcing plate and is fixedly connected to a fixed plate. When in use, the roller is first used to drive the box body 2 to move, and then the electric telescopic rod is used to drive the connecting rod to move, so as to drive the horizontal plate to move. The movement of the horizontal plate drives the limit block to move inside the vertical groove and the vertical rod to move, and the movement of the vertical rod drives the fixed plate to move. When the fixed plate is placed in the place where the box body 2 is placed, the electric telescopic rod is stopped, and the fixed plate is used to support the box body 2.

[0039] The structure and principle of the regulating mechanism 7 composed of a partition, a water spray plate, a heating plate, a temperature and humidity sensor, a lighting lamp, a water pipe, and a solenoid valve in the utility model have been disclosed in an immune cell culture bioreactor disclosed in Chinese Patent Application No. 201922324480.8. Its working principle is: a partition is fixedly connected to the inner wall of the box 2 and is located between the two placement plates 6. The bottom of the partition and the top of the inner cavity of the box 2 are fixedly connected with a water spray plate. The left side of the inner cavity of the box 2 is fixedly connected with a heating plate, and the right side of the inner cavity of the box 2 is fixedly connected with a temperature and humidity sensor. A sensor, a lighting lamp is fixedly connected to the back of the inner cavity of the box body 2, a water pipe is connected to the bottom of the right side of the water tank 1, and the end of the water pipe away from the water tank 1 is connected to the right side of the water spray plate, and a solenoid valve is sleeved on the surface of the water pipe. When in use, the solenoid valve and the heating plate are controlled to work. When the solenoid valve is opened, the water inside the water tank 1 enters the water spray plate through the water pipe, and the water is sprayed out through the water spray plate to increase the humidity inside the box body 2, and the heating plate is used for heating to increase the temperature inside the box body 2, thereby realizing the adjustment operation of the temperature and humidity inside the box body 2.

[0040] The working principle and use process of the utility model are as follows: the utility model uses the mobile support mechanism 4 to move the box body 2 to the cell culture site, installs the end plate 91 on the top of the water tank 1, and drives the connecting frame 93 to move to the top of the fixed frame 95 under the action of the fixed rod 94, so that the filter screen plate 92 can be installed inside the water tank 1, and the operator then opens the end cover on the top of the water injection pipe on the end plate 91, and injects cell culture water into the water tank 1 through the water injection pipe. The injected water can be filtered by the filter screen plate 92 to ensure the cleanliness of the water entering the inside of the regulating mechanism 7, and then the cleanliness of the cell culture water sprayed into the box body 2 can be ensured, and the cell culture effect can be ensured, and then the box door 3 is opened, and the top block 84 is moved up, and the top block 84 moves to drive the connecting rod 83 to move inside the fixed cylinder 81, and the connecting rod 83 moves to drive the first spring 82 to stretch, and then the culture dish 5 is placed on the placement plate 6, The rotating nut 863 is rotated to rotate and move along the fixed screw 862. After the rotating nut 863 moves away from the moving block 864, the second spring 865 loses the external force and returns to its original state, driving the moving block 864 to move. The movement of the moving block 864 drives the insertion rod 866 to move. The movement of the insertion rod 866 drives the pressing block 85 to move. When the pressing block 85 moves to the end position of the culture dish 5, the top block 84 is released, and the first spring 82 loses the external force and returns to its original state, driving the connecting rod 83 to move downward. The connecting rod 83 moves downward to drive the top block 84 to move downward. The top block 84 moves downward to drive the pressing block 85 to move downward to press the culture dish 5 to ensure the stability of the placement of the culture dish 5. Then, the cell culture material is placed in the culture dish 5, and then the box door 3 is closed. The temperature and humidity inside the box 2 are adjusted by the combined action of the adjustment mechanism 7 and the water tank 1 to ensure that the temperature and humidity inside the box 2 meet the cell culture requirements.

[0041] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture bioreactor, comprising a housing, characterized in that: The box body is connected to a water tank above, a water injection assembly is arranged above the water tank, an adjustment mechanism is arranged between the water tank and the box body, a placement plate is connected to the inside of the box body, a culture dish is arranged inside the placement plate, a fixing assembly is arranged between the culture dish and the placement plate, a box door is connected to one side of the water tank, and a movable support mechanism is connected to the bottom of the box body; The fixing assembly includes a fixing cylinder, a first spring, a connecting rod, a top block, a clamping block and a moving part. The inside of the placement plate and both sides of the culture dish are connected with the fixing cylinder, the inside of the fixing cylinder is plugged with a connecting rod, the first spring is connected between the fixing cylinder and the connecting rod, the end of the connecting rod away from the fixing cylinder is connected with the top block, the side of the top block close to the culture dish is provided with a clamping block, and a moving part is provided between the top block and the clamping block.

2. A cell culture bioreactor according to claim 1, characterized in that: Both sides of the connecting rod at one end close to the fixed cylinder are connected to limit sliding blocks, and a limit sliding groove is arranged on the inner side wall of the fixed cylinder at a position corresponding to the limit sliding block.

3. A cell culture bioreactor according to claim 1, characterized in that: The moving part includes an end block, a fixed screw, a rotating nut, a moving block, a second spring and an insertion rod. The end of the clamping block close to the top block is connected to the insertion rod, the end of the insertion rod close to the top block is connected to the moving block, both ends above the top block are connected to the end blocks, a fixed screw is connected between the two end blocks, a through hole is provided on the moving block at a position corresponding to the fixed screw, a second spring is connected between the moving block and the end block and at the periphery of the fixed screw, and a rotating nut is connected on the outer wall of the fixed screw and at the side of the moving block away from the second spring.

4. A cell culture bioreactor according to claim 1, characterized in that: The water injection assembly includes an end plate, a filter screen plate, a connecting frame and a fixing rod. The end plate is arranged above the water tank. Both ends of the end plate close to the water tank are connected to the fixing rods. The end of the fixing rod away from the end plate is connected to the connecting frame. The filter screen plate is connected inside the connecting frame.

5. A cell culture bioreactor according to claim 4, characterized in that: A fixing frame is connected to the inner wall of the water tank and below the connecting frame. Positioning protrusions are connected to both ends of one side of the connecting frame close to the fixing frame. Positioning grooves are arranged inside the fixing frame at positions corresponding to the positioning protrusions.

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