Oscillating device for exosome separation

Abstract

The utility model belongs to the technical field of exosome separation, and particularly relates to an oscillation device for exosome separation, which comprises a bottom plate, a supporting plate is arranged at the top of the bottom plate, a group of C-shaped pipes are fixedly connected to the top of the supporting plate, a group of circular pipes are fixedly connected to the bottom of the supporting plate, and circular rods are fixedly connected into the group of circular pipes through springs. The round rods are slidably connected into the round pipe, the bottoms of the round rods are fixedly connected to the top of the bottom plate, a vibration part is arranged at the top of the bottom plate and used for driving the supporting plate to vibrate, and a fixing assembly is arranged on the supporting plate and used for fixing the centrifugal pipe. The problems that a reagent and exosomes need to be uniformly mixed firstly and then stand in a test tube for precipitation, but the exosomes are extremely viscous, difficult to be fully mixed with the reagent and need to be shaken manually, time and labor are wasted, and if the shaking is insufficient, the cracking time of the exosomes is long are solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of exosome separation, in particular to an oscillating device for exosome separation. Background Art

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Nowadays, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. A variety of cells can secrete exosomes under normal and pathological conditions. They are mainly derived from multivesicular bodies formed by the invagination of intracellular lysosomal microparticles. After the fusion of the outer membrane of the multivesicular body with the cell membrane, they are released into the extracellular matrix. At present, there are few reagents on the market for extracting exosome RNA, and the most commonly used method is RNA precipitation.

[0003] In the prior art, it is necessary to first evenly mix the reagent and exosomes, and then let the test tube stand for precipitation. However, due to the extremely viscous nature of the exosomes, it is difficult to fully mix with the reagents, and manual shaking is required, which is time-consuming and laborious. If the shaking is not sufficient, it will lead to a long exosome lysis time.

[0004] To this end, the utility model provides an oscillating device for separating exosomes. Utility Model Content

[0005] In order to make up for the shortcomings of the existing technology, it is necessary to first mix the reagent and exosomes evenly and then let the test tube stand for precipitation. However, due to the extremely viscous nature of the exosomes, it is difficult to fully mix with the reagents and manual shaking is required, which is time-consuming and laborious. If the shaking is not sufficient, it will lead to a long exosome lysis time.

[0006] The technical solution adopted by the utility model to solve its technical problems is: the utility model is an oscillating device for exosome separation, comprising a bottom plate, a support plate is provided on the top of the bottom plate, a group of C-shaped tubes are fixedly connected to the top of the support plate, a group of circular tubes are fixedly connected to the bottom of the support plate, a group of circular tubes are fixedly connected to a circular rod through a spring in the circular tube, and the circular rod is slidably connected in the circular tube, a group of circular rods are fixedly connected to the top of the bottom plate at the bottom, a vibrating member is provided on the top of the bottom plate, the vibrating member is used to drive the support plate to vibrate, a fixing assembly is provided on the support plate, and the fixing assembly is used to fix the centrifuge tube.

[0007] Preferably, the vibration member includes a first motor, which is fixed to the top of the base plate through a first fixed block, a Z-shaped rod is provided at the output end of the first motor, a first fixed rod is fixed to the bottom of the support plate, a rectangular block is fixed to the bottom of the first fixed rod, a sliding groove is provided on the rectangular block, and the outer circular wall at one end of the Z-shaped rod is slidably connected to the sliding groove.

[0008] Preferably, the fixing assembly includes a power unit and a rotating through slot, a group of the rotating through slots are opened on the support plate, a first rotating rod is rotatably connected in the rotating through slot, an eccentric block is fixedly connected to the top of the first rotating rod, a group of rubber rings are sleeved on the outer circular wall of the eccentric blocks, and the power unit is used to drive a group of first rotating rods to rotate.

[0009] Preferably, the power unit includes a first sprocket, a group of the first sprockets are respectively fixed to the bottom of a group of first rotating rods, the top of the support plate is fixed with a second motor through a pair of L-shaped rods, the output end of the second motor is provided with a driving rod, the bottom of the driving rod is rotatably connected to the top of the support plate, and the bottom of the driving rod passes through the support plate, the bottom of the driving rod is fixed with a second sprocket, and the second sprocket is connected to a group of first sprockets through a chain transmission.

[0010] Preferably, a group of mounting holes are provided on the bottom plate.

[0011] Preferably, a rubber pad is provided on the inner circular wall of the C-shaped tube.

[0012] The beneficial effects of the utility model are as follows:

[0013] 1. The utility model discloses an oscillating device for separating exosomes, which is provided with a circular rod and a circular tube, and the circular rod is fixed in the circular tube by a spring. The provided vibrating member can drive the centrifuge tube containing the exosomes to vibrate, thereby generating vibrations, and vibrating the exosomes, thereby avoiding the extremely viscous nature of the exosomes, which makes it difficult to fully mix with the reagents, and requires manual shaking, which is time-consuming and laborious. If the shaking is not sufficient, it will lead to the problem of long exosome lysis time. The centrifuge tube can be fixed by a fixing component.

[0014] 2. The utility model discloses an oscillating device for separating exosomes, wherein a driving rod is driven to rotate by a second motor, thereby a group of first sprockets are driven to rotate by a second sprocket fixed to the driving rod through the transmission of a chain, thereby the eccentric block is driven to rotate together through the first rotating rod, and the rubber ring is driven to expand at the same time, thereby squeezing the leaked part of the centrifuge tube to achieve a fixing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model will be further described below in conjunction with the accompanying drawings.

[0016] Figure 1 It is a three-dimensional diagram of the utility model;

[0017] Figure 2 It is a schematic diagram of the first fixing rod in the utility model;

[0018] Figure 3 It is a schematic diagram of the second sprocket in the utility model;

[0019] Figure 4 It is a schematic diagram of the first rotating rod in the utility model;

[0020] Figure 5 It is a schematic diagram of the support plate in the utility model;

[0021] In the figure: 1, bottom plate; 2, support plate; 3, C-shaped tube; 4, circular tube; 5, circular rod; 6, first motor; 7, Z-shaped rod; 8, first fixed rod; 9, rectangular block; 10, sliding groove; 11, rotating groove; 12, first rotating rod; 13, eccentric block; 14, rubber ring; 15, first sprocket; 16, second motor; 17, driving rod; 18, second sprocket; 19, chain; 20, mounting hole. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] like Figures 1 to 5 As shown, an oscillating device for separating exosomes described in an embodiment of the utility model comprises a bottom plate 1, a support plate 2 is provided on the top of the bottom plate 1, a group of C-shaped tubes 3 are fixedly connected to the top of the support plate 2, a group of circular tubes 4 are fixedly connected to the bottom of the support plate 2, a group of circular tubes 4 are fixedly connected to a circular rod 5 in the circular tube 4 through a spring, and the circular rod 5 is slidably connected in the circular tube 4, and the bottoms of a group of circular rods 5 are all fixed to the top of the bottom plate 1, a vibrating member is provided on the top of the bottom plate 1, and the vibrating member is used to drive the support plate 2 to vibrate, and a fixing assembly is provided on the support plate 2, and the fixing assembly is used to fix the centrifuge tube. When working, the centrifuge tube containing the exosomes can be driven to vibrate through the provided circular rod 5 and the circular tube 4, and the circular rod 5 is fixed in the circular tube 4 through a spring, so as to generate vibration, and vibrate the exosomes, thereby avoiding the extremely viscous nature of the exosomes, which is difficult to fully mix with the reagent, and requires manual shaking, which is time-consuming and labor-intensive. If the shaking is insufficient, it will lead to the problem of long exosome lysis time. The centrifuge tube can be fixed by the fixing assembly.

[0024] The vibrating member includes a first motor 6, which is fixedly connected to the top of the bottom plate 1 through a first fixed block, a Z-shaped rod 7 is provided at the output end of the first motor 6, a first fixed rod 8 is fixedly connected to the bottom of the support plate 2, a rectangular block 9 is fixedly connected to the bottom of the first fixed rod 8, a sliding groove 10 is provided on the rectangular block 9, and an outer circular wall at one end of the Z-shaped rod 7 is slidably connected in the sliding groove 10. When working, the Z-shaped rod 7 is driven to rotate by the first motor 6, so that one end of the Z-shaped rod 7 can slide in the sliding groove 10, thereby driving the rectangular block 9 to move up and down, and the circular rod 5 is fixedly connected to the circular tube 4 through a spring, so that the support plate 2 can drive the centrifuge tube to oscillate and oscillate the exosomes.

[0025] The fixing assembly includes a power unit and a rotating through groove 11, a group of the rotating through grooves 11 are opened on the support plate 2, a first rotating rod 12 is rotatably connected in the rotating through groove 11, an eccentric block 13 is fixedly connected to the top of the first rotating rod 12, and a rubber ring 14 is sleeved on the outer circular wall of a group of the eccentric blocks 13, and the power unit is used to drive a group of first rotating rods 12 to rotate. When working, a group of first rotating rods 12 are driven to rotate by the power unit, thereby driving a group of eccentric blocks 13 to rotate, so that the rubber ring 14 can be driven to expand through the simultaneous rotation of a group of eccentric blocks 13, so that the centrifuge tube can be squeezed by the rubber ring 14, so as to achieve the fixing effect, that is, the exosomes in the centrifuge tube can be driven to oscillate through vibration.

[0026] The power unit includes a first sprocket 15, a group of the first sprockets 15 are respectively fixed to the bottom of a group of first rotating rods 12, a second motor 16 is fixed to the top of the support plate 2 through a pair of L-shaped rods, and a driving rod 17 is provided at the output end of the second motor 16. The bottom of the driving rod 17 is rotatably connected to the top of the support plate 2, and the bottom of the driving rod 17 passes through the support plate 2, and a second sprocket 18 is fixed to the bottom of the driving rod 17. The second sprocket 18 is connected to a group of first sprockets 15 through a chain 19. When working, the driving rod 17 is driven to rotate by the second motor 16, so that the second sprocket 18 fixed on the driving rod 17 drives a group of first sprockets 15 to rotate through the transmission of the chain 19, so that the eccentric block 13 can be driven to rotate together through the first rotating rod 12, and the rubber ring 14 is driven to expand, so that the leaked part of the centrifuge tube can be squeezed to achieve a fixing effect.

[0027] The base plate 1 is provided with a group of mounting holes 20. During operation, the base plate 1 can be fixed in a fixed position by bolts through the group of mounting holes 20 provided on the base plate 1, so that when the first motor 6 drives the base plate 1 to vibrate, the base plate 1 can be kept stable to prevent the base plate 1 from moving.

[0028] The inner wall of the C-shaped tube 3 is provided with a rubber pad. During operation, when the centrifuge tube is squeezed by the rubber ring 14, the rubber pad can be provided on the inner wall of the C-shaped tube 3 to avoid excessive squeezing that may cause the centrifuge tube to rupture, and the centrifuge tube can be improved in the C-shaped tube 3.

[0029] Working principle: by setting the circular rod 5 and the circular tube 4, and the circular rod 5 is fixedly connected in the circular tube 4 by a spring, the centrifuge tube containing the exosomes can be driven to vibrate by the set vibration piece, thereby generating vibration, and vibrating the exosomes, thereby avoiding the extremely viscous property of the exosomes, which is difficult to fully mix with the reagent, and requires manual vibration, which is time-consuming and labor-intensive. If the vibration is not sufficient, it will lead to the problem of long exosome lysis time. The centrifuge tube can be fixed by the fixing component, and the Z-shaped rod 7 is driven to rotate by the first motor 6, so that one end of the Z-shaped rod 7 can slide in the sliding groove 10, thereby driving the rectangular block 9 to move up and down. The circular rod 5 is fixedly connected in the circular tube 4 by a spring, so that the support plate 2 can drive the centrifuge tube to vibrate, and vibrate the exosomes. A group of first rotating rods 12 are driven to rotate by the set power unit, thereby driving a group of eccentric blocks 13 to rotate, so that a group of eccentric blocks 13 can be driven to rotate at the same time, thereby driving the sleeve The rubber ring 14 is expanded, so that the centrifuge tube can be squeezed by the rubber ring 14, so as to achieve the fixing effect, that is, the exosomes in the centrifuge tube are driven to oscillate by vibration, and the driving rod 17 is driven to rotate by the second motor 16, so that the second sprocket 18 fixed on the driving rod 17 drives a group of first sprockets 15 to rotate through the transmission of the chain 19, so that the eccentric block 13 can be driven to rotate together through the first rotating rod 12, and the rubber ring 14 is driven to expand at the same time, so that the leaked part of the centrifuge tube can be squeezed to achieve the fixing effect, and the bottom plate 1 can be fixed in a fixed position by a group of mounting holes 20 provided on the bottom plate 1 through bolts, so that when the first motor 6 drives to vibrate, the bottom plate 1 can be kept stable to prevent the bottom plate 1 from moving, and the rubber pad is provided on the inner circular wall of the C-shaped tube 3, so that when the centrifuge tube is squeezed by the rubber ring 14, it can avoid excessive squeezing and rupture of the centrifuge tube, and can increase the friction of the centrifuge tube in the C-shaped tube 3.

[0030] The above-mentioned front, back, left, right, top and bottom are all based on the figures in the specification. Figure 1 As a benchmark, according to the person's observation perspective, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0031] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the scope of protection of the present invention.

[0032] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A shaking device for separating exosomes, characterized in that: The invention comprises a bottom plate (1), a support plate (2) is provided on the top of the bottom plate (1), a group of C-shaped tubes (3) are fixedly connected to the top of the support plate (2), a group of circular tubes (4) are fixedly connected to the bottom of the support plate (2), a group of circular tubes (4) are fixedly connected to the inside of the circular tubes (4) via springs, and the circular rods (5) are slidably connected to the inside of the circular tubes (4), the bottoms of the group of circular rods (5) are all fixedly connected to the top of the bottom plate (1), a vibration member is provided on the top of the bottom plate (1), and the vibration member is used to drive the support plate (2) to vibrate, and a fixing component is provided on the support plate (2), and the fixing component is used to fix the centrifuge tube.

2. The oscillating device for separating exosomes according to claim 1, characterized in that: The vibration component comprises a first motor (6), the first motor (6) is fixedly connected to the top of the bottom plate (1) through a first fixing block, a Z-shaped rod (7) is provided at the output end of the first motor (6), a first fixing rod (8) is fixedly connected to the bottom of the support plate (2), a rectangular block (9) is fixedly connected to the bottom of the first fixing rod (8), a sliding groove (10) is provided on the rectangular block (9), and an outer circular wall at one end of the Z-shaped rod (7) is slidably connected in the sliding groove (10).

3. The oscillating device for separating exosomes according to claim 2, characterized in that: The fixing assembly comprises a power unit and a rotating through slot (11), a group of the rotating through slots (11) are arranged on the support plate (2), a first rotating rod (12) is rotatably connected in the rotating through slot (11), an eccentric block (13) is fixedly connected to the top of the first rotating rod (12), a group of rubber rings (14) are sleeved on the outer circular wall of the eccentric block (13), and the power unit is used to drive the group of first rotating rods (12) to rotate.

4. The oscillating device for separating exosomes according to claim 3, characterized in that: The power unit comprises a first sprocket (15), a group of the first sprockets (15) are respectively fixed to the bottom of a group of first rotating rods (12), a second motor (16) is fixed to the top of the support plate (2) via a pair of L-shaped rods, a driving rod (17) is provided at the output end of the second motor (16), the bottom of the driving rod (17) is rotatably connected to the top of the support plate (2), and the bottom of the driving rod (17) passes through the support plate (2), a second sprocket (18) is fixed to the bottom of the driving rod (17), and the second sprocket (18) is connected to the group of first sprockets (15) by a chain (19).

5. The oscillating device for separating exosomes according to claim 4, characterized in that: The bottom plate (1) is provided with a group of mounting holes (20).

6. The oscillating device for separating exosomes according to claim 5, characterized in that: A rubber pad is provided on the inner circular wall of the C-shaped tube (3).

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