Chemiluminescence immunoassay instrument with proofreading function

Abstract

The utility model belongs to the technical field of biomedical treatment, and particularly relates to a chemiluminescence immunoassay instrument. The chemiluminescence immunoassay instrument comprises: an incubation disc module, wherein a reaction cup is movably arranged in the incubation disc module; the laser light source module is arranged above the incubation disc module and is positioned on the motion trail of the reaction cup; the detection module is arranged above the incubation disc module and is positioned on the motion trail of the reaction cup; the reference light source module is arranged below the incubation disc module; and the reference light source module corresponds to the detection module up and down. According to the utility model, the self detection module can be calibrated.

Description

Technical Field

[0001] The utility model belongs to the field of biomedical technology, and in particular relates to a chemiluminescence immunoassay instrument. Background Art

[0002] Luminescence immunoassays combine luminescence with immune responses to detect antigens or antibodies. Using micro-amplification technology, they offer excellent sensitivity and specificity. They can detect a wide range of substances, from traditional proteins, hormones, and enzymes to drugs. Most commonly used in clinical practice are fully automated chemiluminescence immunoassays, fully automated microparticle chemiluminescence immunoassays, and fully automated electrochemiluminescence immunoassays.

[0003] Instrument detection relies directly on the readings of the detection module. However, the detection module may have errors after long-term use, and the readings of the detection module vary from instrument to instrument due to these errors. When comparing data measured by two different instruments, the readings of the detection modules differ, making comparison inconvenient. Utility Model Content

[0004] In response to the above-mentioned technical problems, the present invention aims to provide a chemiluminescence immunoassay instrument that is capable of calibrating its own detection module.

[0005] According to the utility model, a chemiluminescence immunoassay instrument is provided, comprising:

[0006] Incubation tray module;

[0007] A detection module is arranged above the incubation tray module;

[0008] and a reference light source module disposed below the incubation tray module, wherein the reference light source module corresponds to the detection module up and down, and is configured to be able to send a reference light source to the detection module.

[0009] In a preferred embodiment, a laser light source module is further provided above the incubation tray module, and a reaction cup is movably provided in the incubation tray module. The reaction cup is configured to pass under the laser light source module and the detection module in sequence.

[0010] In a preferred embodiment, the reference light source module includes:

[0011] A first fixing seat fixedly arranged below the incubation tray module;

[0012] a first bottom cover fixedly arranged on the bottom of the first fixing base;

[0013] and an LED light source board fixedly arranged in the first fixing seat;

[0014] A channel is provided on the top of the first fixing seat, and the light emitted by the LED light source board is directed toward the detection module through the channel.

[0015] In a preferred embodiment, a first flat lens is provided on the channel of the first fixing seat, and a first sealing member is provided between the first flat lens and the first fixing seat.

[0016] In a preferred embodiment, the detection module is configured to receive the reference light source emitted by the reference light source module, and obtain corresponding reading values ​​according to different intensities of the received reference light source, thereby obtaining a corresponding detection curve.

[0017] In a preferred embodiment, the detection module includes:

[0018] A second base fixedly arranged on the incubation tray module;

[0019] A photomultiplier tube is installed in the second base, and the photomultiplier tube is configured to receive the light source from the reference light source module.

[0020] In a preferred embodiment, the detection module further comprises a second upper cover sealedly disposed on the upper end of the second base, and the second upper cover enables the interior of the second base to be in a light-tight environment.

[0021] In a preferred embodiment, an incubation tray heating plate is provided between the reference light source module and the incubation tray module.

[0022] In a preferred embodiment, a heating plate partition is provided between the incubation tray heating plate and the reference light source module.

[0023] In a preferred embodiment, the incubation tray module comprises:

[0024] An incubation tray body, wherein an incubation tray heating plate is provided at the lower end of the incubation tray body;

[0025] An incubation tray upper cover provided at the upper end of the incubation tray body;

[0026] and an incubation tray lower cover provided at the lower end of the incubation tray body, wherein the incubation tray lower cover fixes the incubation tray heating plate to the lower end portion of the incubation tray body;

[0027] The upper end of the first fixing seat extends to above the lower cover of the incubation tray, and a fixing plate is provided on the side wall of the first fixing seat, the upper end surface of the fixing plate abuts against the lower end surface of the lower cover of the incubation tray, and the fixing plate is fixedly connected to the lower cover of the incubation tray by bolt connection;

[0028] An incubation tray insulation layer is provided on the outer side of the incubation tray body, and the upper and lower ends of the incubation tray insulation layer respectively abut against the incubation tray upper cover and the incubation tray lower cover.

[0029] Compared with the prior art, the advantages of this application are as follows.

[0030] The utility model improves the accuracy of the detection module by setting a reference light source module as a reference calibration, reduces the inter-instrument difference between different instruments, and facilitates the comparison of measurement data between different instruments. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The present invention will be described below with reference to the accompanying drawings.

[0032] Figure 1 A partial top view schematically shows an embodiment of a chemiluminescent immunoassay instrument according to the present invention;

[0033] Figure 2 The figure shows a schematic diagram of the main cross section of the chemiluminescence immunoassay instrument according to the present invention;

[0034] Figure 3 A schematic diagram showing an embodiment of a reference light source module according to the present utility model is shown;

[0035] Figure 4 A schematic diagram showing an embodiment of a detection module according to the present utility model is shown;

[0036] Figure 5 Shows a schematic diagram of the bottom structure of the reference light source module according to the present utility model;

[0037] Figure 6 Shows a schematic diagram of the structure of the incubation plate heating plate.

[0038] In the figure: 100, detection module; 101, photomultiplier tube; 102, second base; 103, second upper cover; 104, second sealing member; 105, second fixing nut; 106, second flat lens; 107, convex lens;

[0039] 200. Laser light source module;

[0040] 300, incubation tray module; 301, incubation tray upper cover; 302, incubation tray body; 303, incubation tray insulation layer; 304, incubation tray lower cover; 305, cuvette motion trajectory; 306, incubation tray heating plate; 307, heating plate partition; 308, inner turntable; 309, outer turntable; 310, inner turntable drive shaft; 311, outer turntable drive shaft;

[0041] 400, reference light source module; 401, LED light source board; 402, first bottom cover; 403, first fixing seat; 404, first sealing member; 405, first flat lens; 406, first fixing nut; 407, channel; 408, fixing plate; 409, step groove;

[0042] 10. Chemiluminescence immunoassay instrument.

[0043] In this application, all drawings are schematic drawings, which are only used to illustrate the principles of the present invention and are not drawn according to the actual scale. DETAILED DESCRIPTION

[0044] The present invention will be described below with reference to the accompanying drawings.

[0045] It should be noted that the directional terms or qualifiers used in this application, "upper" refers to the side of the incubation tray module 300 where the detection module 100 is located, and "lower" refers to the side of the incubation tray module 300 where the reference light source module 400 is located. These terms are not intended to define the absolute positions of the components involved and may vary depending on the specific situation.

[0046] Figure 1 The structure of the chemiluminescence immunoassay instrument 10 according to the present invention is shown in FIG. Figure 1 and Figure 2 As shown, the chemiluminescence immunoassay instrument 10 includes an incubation plate module 300 , a laser light source module 200 , a detection module 100 and a reference light source module 400 .

[0047] A cuvette is movably mounted within the incubation tray module 300. The laser light source module 200 is positioned above the incubation tray module 300 and along the cuvette's trajectory. This means that the cuvette can move below the laser light source module 200 during movement, allowing the laser light emitted by the laser light source module 200 to illuminate the cuvette.

[0048] The detection module 100 is disposed above the incubation tray module 300 and is located on the movement trajectory of the reaction cup. In other words, the reaction cup can move to the bottom of the detection module 100 during movement, and the detection module 100 can perform detection on the inside of the reaction cup.

[0049] Furthermore, the detection module 100 is located downstream of the laser light source module 200. That is, during the movement of the reaction cup, it starts from the initial position, first passes through the laser light source module 200, and then passes through the detection module 100, so that the detection module 100 can detect the substance in the reaction cup irradiated by the laser light source module 200.

[0050] In this embodiment, the chemiluminescence immunoassay instrument 10 is circular in shape as a whole, that is, the incubation tray module 300 is circular in shape as a whole. Figure 1 The structure of half of the chemiluminescent immunoassay instrument 10 is shown, combined with Figure 1 The motion trajectory of the cuvette in the incubation tray module 300 is the cuvette motion trajectory 305 , and the center of the cuvette motion trajectory 305 is on the central axis of the incubation tray module 300 .

[0051] The laser emission port of the laser light source module 200 and the detection port of the detection module 100 are both located on the cuvette motion trajectory 305 and are perpendicular to the cuvette to be tested. During operation, the cuvette to be tested rotates within the incubation tray module 300 and moves below the laser light source module 200. During each movement cycle, the laser light source module 200 emits laser light to excite the chemical substances in the cuvette directly below. During the next movement cycle, the cuvette to be tested moves below the detection module 100, where it detects and reads the luminescence value of the cuvette to be tested.

[0052] In a specific embodiment, the laser light source module 200 includes a laser generator.

[0053] According to the present invention, the reference light source module 400 is disposed below the incubation tray module 300, and the reference light source module 400 corresponds to the detection module 100 above and below. In this configuration, the reference light source module 400 can transmit a reference light source to the detection module 100, while the detection module 100 can receive the reference light source transmitted by the reference light source module 400 and obtain corresponding readings based on the different intensities of the received reference light source, thereby obtaining a corresponding detection curve.

[0054] When the chemiluminescent immunoassay instrument 10 is operating normally, the reference light source module 400 is not required. When the prescribed maintenance time is reached or there is doubt about the test results, the reference light source module 400 can be used to calibrate and verify the readings of the detection module 100.

[0055] During calibration, there is no reaction cup blocking the incubation tray module 300 , and the light emitted by the reference light source module 400 can directly reach the detection module 100 for reading.

[0056] The reference light source module 400 can adjust LED lights of different intensities, and can obtain reading values ​​of the detection module 100 under different light intensities, thereby obtaining a detection curve of the detection module 100 .

[0057] The detection curves obtained by different instruments are certainly different. Through software compensation, the detection curves of different instruments can be made as close as possible, reducing the inter-instrument differences. It is easy to understand that the method of compensation by software is a prior art and is not the technical point of the present invention.

[0058] According to the present invention, in a specific embodiment, Figure 2 、 Figure 3 and Figure 5 As shown, the reference light source module 400 includes a first fixing base 403 fixedly disposed below the incubation tray module 300, a first bottom cover 402 fixedly disposed at the bottom of the first fixing base 403, and an LED light source board 401 fixedly disposed within the first fixing base 403. In this embodiment, the LED light source board 401 is sealed and fixed to the lower end of the first fixing base 403 via the first bottom cover 402, preventing light emitted from the LED light source board 401 from escaping from below.

[0059] In a preferred embodiment, a step groove 409 is provided between the contact surfaces of the first bottom cover 402 and the first fixing seat 403 . The provision of the step groove 409 can enhance the sealing performance between the two.

[0060] In this embodiment, a channel 407 is opened on the top of the first fixing base 403 , and the light emitted by the LED light source board 401 is directed toward the detection module 100 through the channel 407 .

[0061] In a specific embodiment, a first flat lens 405 is provided on the channel 407 of the first fixing seat 403 , and an end surface of the first flat lens 405 is perpendicular to the axis of the channel 407 .

[0062] A first seal 404 is provided between the first flat lens 405 and the first fixed seat 403. The first seal 404 is located between the first fixed seat 403 and the axial abutment surface of the first flat lens 405. The first flat lens 45 enters the first fixed seat 403 from above the first fixed seat 403, and then a first fixing nut 406 for fixing the first flat lens 405 is provided on the first fixed seat 403 by a threaded connection. The first fixing nut 406 is connected to the first fixed seat 403 by a threaded connection. After the first fixing nut 406 is tightened, the lower end of the first fixing nut 406 abuts against the upper end surface of the first flat lens 405. The first seal 404 can prevent the first flat lens 405 from being crushed when the first fixing nut 406 fixes the first flat lens 405, and at the same time plays the role of sealing light to prevent light leakage.

[0063] According to the present invention, in a specific embodiment, the incubation tray module 300 includes an incubation tray body 302 , an incubation tray upper cover 301 disposed at the upper end of the incubation tray body 302 , and an incubation tray lower cover 304 disposed at the lower end of the incubation tray body 302 .

[0064] In this embodiment, the upper end of the first fixing seat 403 extends above the incubation tray lower cover 304 and at least partially enters the incubation tray body 302. Specifically, the first fixing seat 403 is installed upward from the bottom of the incubation tray lower cover 304, and the upper end of the first fixing seat 403 passes through the incubation tray lower cover 304 and reaches into the incubation tray body 302. The channel 407 connects the space where the LED light source board 401 is located with the internal space of the incubation tray body 302, so that light emitted by the LED light source board 401 is illuminated by the channel 407 toward the detection module 100.

[0065] In this embodiment, if Figure 2 and Figure 6 As shown, an incubation tray heater 306 and a heater separator 307 are sequentially disposed at the bottom of the incubation tray 302. Specifically, the incubation tray heater 306 and the heater separator 307 are disposed between the incubation tray 302 and the first fixing base 403 of the reference light source module 400. The incubation tray heater 306 heats the incubation tray 302, thereby heating the air within the incubation tray 302 to provide the required ambient temperature for incubation.

[0066] like Figure 2 As shown, the incubation tray module 300 further includes an inner turntable 308, an outer turntable 309, an inner turntable drive shaft 310, and an outer turntable drive shaft 311. The inner turntable 308, the outer turntable 309, the inner turntable drive shaft 310, and the outer turntable drive shaft 311 are all coaxially rotatably disposed within the incubation tray module 300. The inner turntable drive shaft 310 is rotatably sleeved within the outer turntable drive shaft 311, and the inner turntable 308 is rotatably sleeved within the outer turntable 309, with the inner turntable 308 being located above the outer turntable 309. The inner ring turntable 308 is fixedly connected to the inner ring turntable drive shaft 310, and the outer ring turntable 309 is fixedly connected to the outer ring turntable drive shaft 311. The inner ring turntable drive shaft 310 can drive the inner ring turntable 308 to rotate, and the outer ring turntable drive shaft 311 can drive the outer ring turntable 309 to rotate.

[0067] The cuvette can be placed on the inner turntable 308 and the outer turntable 309, and rotated by the inner turntable drive shaft 310 and the outer turntable drive shaft 311 to form a cuvette motion track 305. Figure 2 As shown, holes are provided at the bottom of the inner turntable 308 where the reaction cup is placed to facilitate the passage of the pipeline of the reference light source module 400, and holes are also provided at corresponding positions of the outer turntable 309. In a preferred embodiment, the diameter of the hole on the outer turntable 309 is greater than or equal to the diameter of the hole on the inner turntable 308.

[0068] In a preferred embodiment, a fixing plate 408 is provided on the side wall of the first fixing seat 403. The fixing plate 408 is flat and arranged in the horizontal direction. The upper end surface of the fixing plate 408 abuts against the lower end surface of the incubation tray lower cover 304, and the fixing plate 408 is fixedly connected to the incubation tray lower cover 304 by bolt connection.

[0069] In a preferred embodiment, an incubation tray insulation layer 303 is provided on the outside of the incubation tray body 302. The upper and lower ends of the incubation tray insulation layer 303 abut against the incubation tray upper cover 301 and the incubation tray lower cover 304 respectively.

[0070] In a specific embodiment, Figure 4 As shown, the detection module 100 includes a photomultiplier tube 101, a second base 102, a second upper cover 103, a second seal 104, a second fixing nut 105, a second flat lens 106 and a convex lens 107. The second base 102 is fixedly set on the incubation tray upper cover 301, and the photomultiplier tube 101 is installed in the second base 102. The second upper cover 103 is set at the upper end of the second base 102 to seal the second base 102, thereby providing a relatively lightless environment and preventing the ambient light from affecting the readings of the detection module 100. A circular through hole corresponding to the photomultiplier tube 101 is reserved at the bottom of the second base 102 for mounting the second flat lens 106 and the convex lens 107. The central axes of the second flat lens 106 and the convex lens 107 coincide, and the central axes of the second flat lens 106, the convex lens 107 and the first flat lens 405 coincide. The second fixing nut 105 is used to fix the second flat lens. A second sealing member 104 is installed between the axial contact surface of the second flat lens 106 and the photomultiplier tube 101 to prevent the second flat lens 106 from being crushed when being fixed and to perform a sealing function.

[0071] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of this utility model, "plurality" means two or more, unless otherwise specifically defined.

[0072] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integral connection; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0073] Throughout this specification, reference to terms such as "one embodiment," "some embodiments," "examples," "specific examples," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0074] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and does not constitute any limitation to the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A chemiluminescent immunoassay instrument, characterized in that: include: Incubation tray module (300); A detection module (100) disposed above the incubation tray module (300); and a reference light source module (400) disposed below the incubation tray module (300), wherein the reference light source module (400) corresponds to the detection module (100) in upper and lower positions, and the reference light source module (400) is configured to be able to send a reference light source to the detection module (100).

2. The chemiluminescent immunoassay instrument according to claim 1, characterized in that: A laser light source module (200) is also arranged above the incubation tray module (300), and a reaction cup is movably arranged in the incubation tray module (300), and the reaction cup is configured to pass successively under the laser light source module (200) and the detection module (100).

3. The chemiluminescent immunoassay instrument according to claim 1, characterized in that: The reference light source module (400) comprises: A first fixing seat (403) fixedly arranged below the incubation tray module (300); A first bottom cover (402) fixedly disposed at the bottom of the first fixing seat (403); and an LED light source board (401) fixedly arranged in the first fixing seat (403); A channel (407) is provided on the top of the first fixing seat (403), and the light emitted by the LED light source board (401) is irradiated toward the detection module (100) through the channel.

4. The chemiluminescent immunoassay instrument according to claim 3, characterized in that: A first flat lens (405) is arranged on the channel (407) of the first fixing seat (403), and a first sealing member (404) is arranged between the first flat lens (405) and the first fixing seat (403).

5. The chemiluminescent immunoassay instrument according to claim 1, characterized in that: The detection module (100) is configured to receive the reference light source emitted by the reference light source module (400), and obtain corresponding reading values ​​according to different intensities of the received reference light source, thereby obtaining a corresponding detection curve.

6. The chemiluminescent immunoassay instrument according to claim 5, characterized in that: The detection module (100) comprises: A second base (102) fixedly arranged on the incubation tray module (300); A photomultiplier tube (101) is installed in the second base (102), and the photomultiplier tube (101) is configured to receive the light source from the reference light source module (400).

7. The chemiluminescent immunoassay instrument according to claim 6, characterized in that: The detection module (100) further comprises a second upper cover (103) which is sealed and arranged on the upper end of the second base (102); the second upper cover (103) enables the second base (102) to be in a light-tight environment.

8. The chemiluminescent immunoassay instrument according to any one of claims 1 to 7, characterized in that: An incubation tray heating plate (306) is provided between the reference light source module (400) and the incubation tray module (300).

9. The chemiluminescent immunoassay instrument according to claim 8, characterized in that: A heating plate partition (307) is provided between the incubation tray heating plate (306) and the reference light source module (400).

10. The chemiluminescent immunoassay instrument according to claim 3 or 4, characterized in that: The incubation tray module (300) comprises: An incubation tray body (302), wherein an incubation tray heating plate (306) is provided at the lower end of the incubation tray body (302); An incubation tray upper cover (301) disposed on the upper end of the incubation tray body (302); and an incubation tray lower cover (304) disposed at the lower end of the incubation tray body (302), wherein the incubation tray lower cover (304) fixes the incubation tray heating plate (306) to the lower end of the incubation tray body (302); The upper end of the first fixing seat (403) extends to the upper side of the lower cover (304) of the incubation tray, and a fixing plate (408) is provided on the side wall of the first fixing seat (403), the upper end surface of the fixing plate (408) abuts against the lower end surface of the lower cover (304) of the incubation tray, and the fixing plate (408) is fixedly connected to the lower cover (304) of the incubation tray by means of bolt connection; An incubation tray insulation layer (303) is arranged on the outer side of the incubation tray body (302), and the upper and lower ends of the incubation tray insulation layer (303) respectively abut against the incubation tray upper cover (301) and the incubation tray lower cover (304).

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