Oocyte grading egg picking device

Abstract

The utility model relates to the technical field of oocyte sorting, in particular to an oocyte grading ovum picking device which comprises an ovum picking vessel and an ovum containing vessel, the ovum containing vessel is located inside the ovum picking vessel and fixed on the bottom face of the ovum picking vessel, the height of the ovum containing vessel is lower than that of the ovum picking vessel, the ovum containing vessel is in a circular shape, and the ovum containing vessel is in a circular shape. The bottoms of the four egg containing vessels are marked as A, B, C and D respectively. According to the egg picking device, cold stress damage, hyperosmosis damage and mechanical damage during egg picking in the prior art are effectively reduced. And the working efficiency is improved in the egg picking process.

Description

Technical Field

[0001] The utility model relates to the technical field of oocyte sorting, in particular to an oocyte grading and egg picking device. Background Art

[0002] In the process of obtaining animal oocytes, it is necessary to use oocyte picking tubes (usually glass or plastic products, with a diameter slightly larger than oocytes) to screen out oocytes that meet the requirements one by one from a large number of tissue fragments and cells. Generally, an oocyte picking dish is required, which has a tissue mixture containing oocytes, and another cell culture dish (egg holding dish) is used to hold oocytes that meet the requirements. In the process of selecting oocytes, it is necessary to continuously use oocyte picking tubes to suck oocytes from the oocyte picking dish to the egg holding dish. In this process, oocytes are susceptible to damage in the following aspects: 1. Cold stress, the ambient temperature is lower than the temperature in the culture dish, and the temperature of oocytes in the oocyte picking tube drops rapidly, affecting developmental potential; 2. High osmotic pressure damage, the oocyte picking tube is slightly larger than the oocyte, the liquid volume is very low, the liquid evaporates quickly, the osmotic pressure becomes high, and the developmental potential is affected; 3. Mechanical damage, the oocyte picking tube repeatedly moves from the liquid surface of the oocyte picking dish to the surface of the egg holding dish, which is easy to produce bubbles in it, and the oocytes in the tube are mechanically squeezed many times, affecting their developmental potential. Summary of the invention

[0003] The utility model aims to provide an oocyte grading and egg picking device to solve the problems raised in the above-mentioned background technology.

[0004] In the present application, the oocyte grading egg picking device comprises an egg picking dish and an egg holding dish. The egg holding dish is located inside the egg picking dish and fixed on the bottom surface of the egg picking dish. The height of the egg holding dish is lower than that of the egg picking dish.

[0005] Furthermore, the egg collecting dish is square or round.

[0006] Furthermore, the egg holding dish is square or round.

[0007] Furthermore, the height of the egg holding dish is 1 / 2-2 / 3 of the height of the egg picking dish.

[0008] Furthermore, the egg holding dish is integrally formed with the egg holding dish or is detachable.

[0009] Furthermore, the egg-containing dishes are three circular ones, and the bottoms of the three egg-containing dishes are marked as A, B, and C respectively.

[0010] Furthermore, the egg-containing dishes are four circular ones, and the bottoms of the four egg-containing dishes are marked as A, B, C, and D respectively.

[0011] Furthermore, the egg-containing dishes are three square dishes, and the bottoms of the three egg-containing dishes are marked as A, B, and C respectively.

[0012] Furthermore, the egg-containing dishes are four square dishes, and the bottoms of the four egg-containing dishes are marked as A, B, C, and D respectively.

[0013] Furthermore, a buckle cooperating with the egg picking dish is arranged at the bottom of the egg holding dish, or a suction cup is arranged at the bottom of the egg holding dish.

[0014] Further, only one egg dish may be provided, and the eggs picked are placed in an egg dish, and then removed with a pipette after completion.

[0015] Usage: Pour the tissue mixture containing oocytes into the egg picking dish. The liquid height does not exceed the height of the egg holding dish. At this time, the egg holding dish is empty. Then pour the cleaning liquid into the egg holding dish. After it is full, continue to add the cleaning liquid. The cleaning liquid overflows into the egg picking dish until the liquid level in the egg picking dish is higher than a certain height of the egg holding dish. Then start picking eggs with the egg picking capillary under the microscope and put the collected oocytes into the egg holding dish. The oocyte grading can also be completed during the egg picking process. The operator places the picked oocytes into the corresponding egg picking dish according to the grading standards. The A-level oocytes are placed in the egg holding dish marked with A, the B-level oocytes are placed in the egg holding dish with B, the C-level oocytes are placed in the egg holding dish with C, and the D-level oocytes are placed in the egg holding dish with D. After the sorting is completed, the oocytes are moved to other dishes at one time, or the egg holding dish can be taken away directly. Specifically, the liquid in the egg picking dish can be drained out first, and then the egg picking dish can be taken out after the liquid level in the egg picking dish is lower than the eggs.

[0016] By adopting the above technical solution, the egg picking process is always carried out under the liquid level. Compared with the prior art, the beneficial effects of the utility model are:

[0017] 1. Effectively reduce the cold stress damage, high osmotic pressure damage and mechanical damage during egg picking in the prior art.

[0018] 2. During the egg picking process, the oocyte grading can be completed at the same time, which improves the work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the egg picker in the first embodiment of the utility model;

[0020] Figure 2 for Figure 1 A schematic diagram of the structure of the middle egg collecting device from a top view;

[0021] Figure 3 This is a schematic diagram of the structure of the egg picker in the second embodiment of the utility model;

[0022] Figure 4 for Figure 3A schematic diagram of the structure of the middle egg collecting device from a top view;

[0023] In the picture: 1. Picking up the egg dish; 2. Holding the egg dish. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Example 1

[0026] like Figure 1-2 The utility model discloses an oocyte grading and egg picking device, comprising an egg picking dish 1 and an egg holding dish 2, wherein the egg holding dish 2 is located inside the egg picking dish 1 and fixed on the bottom surface of the egg picking dish 1, and the height of the egg holding dish 2 is lower than the height of the egg picking dish 1. The egg picking dish 1 is round, and can also be set to a square shape as required. The egg holding dish is round, and can also be set to a square shape as required. The height of the egg holding dish 2 is 1 / 2-2 / 3 of the height of the egg picking dish. The egg holding dishes are 4 round, and the bottoms of the 4 egg holding dishes 2 are marked as A, B, C, and D respectively to indicate the grade of the oocytes. The operator can complete the grading of the oocytes at the same time when picking the eggs, thereby improving the work efficiency. Further, the egg holding dish 2 is integrally formed with the egg picking dish 1 or is detachable. Specifically, a buckle that matches the bottom surface of the egg picking dish 1 can be provided at the bottom of the egg holding dish 1, or a suction cup can be directly provided on the bottom surface of the bottom of the egg holding dish 2, and the suction cup can be used to fix the egg holding dish 2. The egg picking dish 1 can be modified appropriately using an existing culture dish, or the material of an existing culture dish can be used to form an integral part when making the egg picking dish 1. The four egg holding dishes 2 can be arranged to be tangential or non-tangential, and for ease of use, the egg holding dishes 2 can be centrally arranged at the bottom center of the egg picking dish 1.

[0027] Example 2

[0028] like Figure 3-4 On the basis of the embodiment, the egg picking device is further improved. The egg picking dish 1 is designed to be square, and the egg holding dishes 2 are 4 square, and the bottoms of the 4 egg holding dishes 2 are marked as A, B, C, and D respectively.

[0029] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention of the utility model.

Claims

1. An oocyte grading and egg picking device, characterized in that: The egg collecting device comprises an egg collecting dish and an egg containing dish. The egg containing dish is located inside the egg collecting dish and fixed on the bottom surface of the egg collecting dish. The height of the egg containing dish is lower than that of the egg collecting dish. There are four egg containing dishes, and the bottoms of the four egg containing dishes are marked as A, B, C, and D respectively.

2. The oocyte grading and egg picking device according to claim 1, characterized in that: The egg collecting dish is square or round.

3. The oocyte grading and egg picking device according to claim 1, characterized in that: The egg-holding dish is square or circular.

4. The oocyte grading and egg picking device according to claim 1, characterized in that: The height of the egg-holding dish is 1 / 2-2 / 3 of the height of the egg-picking dish.

5. The oocyte grading and egg picking device according to claim 1, characterized in that: The egg holding dish is integrally formed with the egg holding dish or is detachable.

6. The oocyte grading and egg picking device according to claim 5, characterized in that: The egg-containing dishes are three circular dishes, and the bottoms of the three egg-containing dishes are marked as A, B, and C respectively.

7. The oocyte grading and egg picking device according to claim 5, characterized in that: The egg-holding dish is circular.

8. The oocyte grading and egg picking device according to claim 5, characterized in that: The egg-containing dishes are three square dishes, and the bottoms of the three egg-containing dishes are marked as A, B, and C respectively.

9. The oocyte grading and egg picking device according to claim 5, characterized in that: The egg-containing dishes are four square dishes, and the bottoms of the four egg-containing dishes are marked as A, B, C, and D respectively.

10. The oocyte grading and egg picking device according to claim 5, characterized in that: The bottom of the egg holding dish is provided with a buckle matched with the egg picking dish, or the bottom of the egg holding dish is provided with a suction cup.

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