Multi-photon microscope with lens protection

Abstract

The utility model discloses a multiphoton microscope with lens protection, which comprises a microscope seat, a microscope arm mounted on the microscope seat, an adjusting seat arranged at the top end of the microscope arm, a coarse focusing screw mounted on one side of the adjusting seat, a fine focusing screw mounted on the other side of the adjusting seat, a multiphoton lens barrel arranged on one side of the adjusting seat, and an eyepiece arranged at the top of the multiphoton lens barrel. An objective lens is installed at the bottom of the multi-photon lens cone, an objective table is arranged below the objective lens and arranged on one side of the lens arm, a moving mechanism is arranged on one side of the objective table, a lower dust shielding cover is arranged at one end of the moving mechanism, a plurality of dust shielding grooves are formed in the lower dust shielding cover and used for being matched with an objective lens, and a light chopper is installed at the bottom of the objective table. The bottom of the lens arm is also provided with a mounting rack, the mounting rack is internally provided with a light source generator, and the light source generator is used for illumination detection. The microscope has the advantages that the eyepiece and the objective lens are protected, the lens is prevented from being damaged, the overall structure is simple and convenient to disassemble and assemble, and the objective table is protected to a certain extent.

Description

Technical Field

[0001] The utility model belongs to the technical field of microscopes, and more specifically, particularly relates to a multi-photon microscope with lens protection. Background Art

[0002] At present, optical microscopy is a common scientific tool, widely used in medicine, biology, materials science and other fields. However, traditional optical microscopes have certain limitations, especially when observing deep tissues or living samples, their resolution and light depth are limited. To overcome these problems, scientists have developed multiphoton microscopy, whose principle is based on nonlinear optical effects. Multiphoton microscopy uses high-power, ultrashort pulse lasers to achieve microscopic imaging. When the laser interacts with the sample, the energy of the photons is absorbed or scattered, forming a nonlinear effect. These nonlinear effects cause the light to generate high-energy photons inside the sample and change the propagation direction of the light. Multiphoton microscopy achieves imaging by exciting fluorescent substances in the sample. When the laser interacts with the sample, the fluorescent substance absorbs the laser energy and is in an excited state. During the deexcitation process, the fluorescent substance emits long-wavelength fluorescent photons. Multiphoton microscopy uses devices to collect and detect these fluorescent photons and generate images. Compared with traditional fluorescence microscopy, multiphoton microscopy has better deep imaging capabilities and higher spatial resolution.

[0003] Existing multiphoton microscopes do not have a lens protection structure during use, and the external lens cover is easy to lose, resulting in the inability to protect the lens after the test is completed. The eyepiece and objective lens cannot receive subsequent protection. At the same time, the stage is exposed for a long time and is easily bumped, causing damage to the stage. Utility Model Content

[0004] In view of the deficiencies in the prior art, the utility model provides a multi-photon microscope with lens protection to solve the problems raised in the above background technology.

[0005] To achieve the above purpose, the utility model is implemented through the following technical solutions: a multi-photon microscope with lens protection, including a mirror seat, a mirror arm is installed on the mirror seat, an adjustment seat is arranged on the top of the mirror arm, a coarse focusing screw is installed on one side of the adjustment seat for preliminary adjustment of the focal length, and a fine focusing screw is installed on the other side for fine adjustment of the focal length, a multi-photon lens barrel is arranged on one side of the adjustment seat, an eyepiece is arranged on the top of the multi-photon lens barrel for personnel observation, an objective lens is installed at the bottom of the multi-photon lens barrel for adjusting the lens size, a stage is arranged below the objective lens, the stage is arranged on one side of the mirror arm, symmetrical pressing clips are installed on the stage, a moving mechanism is arranged on one side of the stage, a lower dust cover is arranged at one end of the moving mechanism, and a plurality of dust shielding grooves are arranged on the lower dust cover for matching the objective lens, a light shield is installed at the bottom of the stage, and light brightness is adjusted by the light shield, a mounting frame is also arranged at the bottom of the mirror arm, a light source generator is arranged in the mounting frame, and light detection is performed by the light source generator.

[0006] As an optional solution of the utility model, the moving mechanism includes a moving seat, which is fixed on the mirror arm, and an adjusting screw is installed in the moving seat through a bearing. A spiral block is installed at one end of the adjusting screw for controlling the rotation of the adjusting screw. A moving block is threadedly assembled on the adjusting screw, and the moving block is slidably fitted in a slide groove of the moving seat. One side of the moving block is connected to a lower dust cover through a damping hinge.

[0007] As an optional solution of the present invention, a placement groove is provided in the mirror base, and the placement groove is used for storing the object stage.

[0008] As an optional solution of the utility model, a telescopic rod is arranged on the top of the adjustment seat, an upper dust cover is installed on the top of the telescopic rod, and a card slot is arranged on the bottom of the upper dust cover, and the card slot is adapted to the eyepiece.

[0009] As an optional solution of the utility model, the stage is engaged with a boss on one side of the mirror arm and is installed and fixed by fixing bolts.

[0010] The utility model provides a multi-photon microscope with lens protection, which has the following beneficial effects:

[0011] The upper dust cover is rotated by adjusting the telescopic rod, and the adjusting screw is driven to rotate by rotating the screw block, so that the moving block drives the lower dust cover to move to the non-interference area to facilitate observation. After the inspection is completed, the stage is quickly disassembled and assembled through the fixing screw and stored in the placement slot to further protect the stage. Then the upper dust cover and the lower dust cover are matched on the eyepiece and the objective lens to protect the lens. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the utility model;

[0013] Figure 2 It is a side view of the utility model;

[0014] Figure 3 It is a cross-sectional view of the utility model.

[0015] In the figure: 1. mirror base; 101. placement slot; 2. mirror arm; 3. adjustment base; 301. coarse focusing screw; 302. fine focusing screw; 4. multiphoton lens barrel; 401. eyepiece; 402. objective lens; 5. stage; 501. film clamp; 502. light shield; 6. upper dust cover; 601. telescopic rod; 7. moving base; 8. adjustment screw; 801. screw block; 9. moving block; 10. lower dust cover; 11. fixing bolt; 12. mounting bracket; 121. light source generator. DETAILED DESCRIPTION

[0016] The following is a further detailed description of the implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0017] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0018] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0019] See also Figures 1 to 3The utility model provides a technical solution: a multi-photon microscope with lens protection, comprising a lens seat 1, a lens arm 2 is installed on the lens seat 1, an adjusting seat 3 is arranged on the top of the lens arm 2, a coarse focusing screw 301 is installed on one side of the adjusting seat 3 for preliminary adjustment of the focal length, and a fine focusing screw 302 is installed on the other side for fine adjustment of the focal length, a multi-photon lens barrel 4 is arranged on one side of the adjusting seat 3, an eyepiece 401 is arranged on the top of the multi-photon lens barrel 4 for personnel observation, an objective lens 402 is installed at the bottom of the multi-photon lens barrel 4 for adjusting the lens size, a stage 5 is arranged below the objective lens 402, the stage 5 is snapped on the boss on one side of the lens arm 2, and is installed and fixed by fixing bolts 11, a symmetrical pressing clip 501 is installed on the stage 5, a moving mechanism is arranged on one side of the stage 5, a lower dust cover 10 is arranged at one end of the moving mechanism, and a plurality of dust shielding grooves are arranged on the lower dust cover 10 for cooperating with the objective lens 402 to protect all the lenses of the objective lens 402.

[0020] The moving mechanism includes a moving seat 7, which is fixed on the mirror arm 2. An adjusting screw 8 is installed in the moving seat 7 through a bearing. A spiral block 801 is installed at one end of the adjusting screw 8 for controlling the rotation of the adjusting screw 8. A moving block 9 is threadedly assembled on the adjusting screw 8. The moving block 9 slides in the slide groove of the moving seat 7. One side of the moving block 9 is connected to the lower dust cover 10 through a damping hinge. A placement groove 101 is provided in the mirror seat 1. The placement groove 101 is used to store the stage 5 to ensure the overall integrity of the stage 5 and avoid bumps.

[0021] A shade 502 is installed at the bottom of the stage 5, and the brightness is adjusted by the shade 502. A mounting frame 12 is also provided at the bottom of the mirror arm 2, and a light source generator 121 is provided in the mounting frame 12, and light detection is performed by the light source generator 121. A telescopic rod 601 is provided on the top of the adjustment seat 3, and an upper dust cover 6 is installed on the top of the telescopic rod 601. A card slot is provided at the bottom of the upper dust cover 6, and the card slot is adapted to the eyepiece 401, so as to protect the eyepiece 401 as a whole. When in use, the upper dust cover 6 is lifted and rotated to the unobstructed area, and the lower dust cover 10 below is also adjusted to a position that does not interfere with the use of the objective lens 402.

[0022] Specific usage and function of this embodiment: when observation is required, adjust the telescopic rod 601 and rotate the upper dust cover 6. At the same time, adjust the height through the coarse focusing screw 301 of the adjustment seat 3, so that the lower dust cover 10 leaves the objective lens 402, and the rotating screw block 801 drives the adjusting screw 8 to rotate, so that the moving block 9 drives the lower dust cover 10 to move to the non-interference area to facilitate observation. After the inspection is completed, the stage 5 is quickly disassembled and assembled through the fixing screw and stored in the placement slot 101 to further protect the stage 5. Then, the upper dust cover 6 and the lower dust cover 10 are matched on the eyepiece 401 and the objective lens 402 to protect the lens.

[0023] The device embodiments described above are merely illustrative, wherein the units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the scheme of this embodiment. Ordinary technicians in this field can understand and implement it without paying creative labor.

[0024] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. A multiphoton microscope with lens protection, characterized in that: The invention comprises a mirror base (1), wherein a mirror arm (2) is mounted on the mirror base (1), an adjustment base (3) is arranged at the top of the mirror arm (2), a coarse focusing screw (301) is mounted on one side of the adjustment base (3) for preliminary adjustment of the focal length, and a fine focusing screw (302) is mounted on the other side for fine adjustment of the focal length, a multiphoton lens barrel (4) is arranged on one side of the adjustment base (3), an eyepiece (401) is arranged on the top of the multiphoton lens barrel (4) for personnel observation, an objective lens (402) is mounted on the bottom of the multiphoton lens barrel (4) for adjusting the lens size, and a stage (5) is arranged below the objective lens (402), and the stage (5) is arranged on one side of the mirror arm (2), a symmetrical sheet pressing clamp (501) is installed on the object platform (5), a moving mechanism is arranged on one side of the object platform (5), a lower dust cover (10) is arranged at one end of the moving mechanism, and a plurality of dust shielding grooves are arranged on the lower dust cover (10) for matching with the lens of the objective lens (402), a shade (502) is installed at the bottom of the object platform (5), and brightness adjustment is performed through the shade (502), and a mounting frame (12) is also arranged at the bottom of the mirror arm (2), and a light source generator (121) is arranged in the mounting frame (12), and light detection is performed through the light source generator (121).

2. A multi-photon microscope with lens protection according to claim 1, characterized in that: The moving mechanism comprises a moving seat (7), wherein the moving seat (7) is fixed to the mirror arm (2), an adjusting screw (8) is installed in the moving seat (7) via a bearing, a spiral block (801) is installed at one end of the adjusting screw (8) for controlling the rotation of the adjusting screw (8), a moving block (9) is threadedly assembled on the adjusting screw (8), the moving block (9) is slidably fitted in a slide groove of the moving seat (7), and one side of the moving block (9) is connected to a lower dust cover (10) via a damping hinge.

3. The multi-photon microscope with lens protection according to claim 1, characterized in that: A placement groove (101) is provided in the mirror base (1), and the placement groove (101) is used to store the object carrier (5).

4. The multi-photon microscope with lens protection according to claim 1, characterized in that: A telescopic rod (601) is arranged on the top of the adjustment seat (3), an upper dust cover (6) is installed on the top of the telescopic rod (601), and a card slot is arranged on the bottom of the upper dust cover (6), and the card slot is adapted to fit the eyepiece (401).

5. The multi-photon microscope with lens protection according to claim 1, characterized in that: The object carrier (5) is engaged with a boss on one side of the mirror arm (2) and is installed and fixed by means of fixing bolts (11).

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