Cell culture medium adder
By designing a cell culture medium adder containing pipetting structure and stirring structure, the contamination problem during cell fluid transfer during cell culture is solved, and the effect of transfer and cleaning and disinfection is achieved without opening the storage container.
Patent Information
- Application Number
- CN202420556265.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-21
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-03-21
AI Technical Summary
During cell culture, the prior art requires frequent opening of storage containers for extraction and transfer of cell fluid, which can easily lead to external contamination and affect the purity and quality of cells.
A cell culture medium adder is designed, including a cell fluid storage box and a pipette. Through the pipetting structure, the cell fluid can be transferred to the pipette without opening the storage container, and cleaned and disinfected by a stirring structure.
The transfer of cell fluid can be completed without opening the storage container, reducing the risk of contamination, and ensuring the cleaning and disinfection of the interior of the container through the stirring structure.
Smart Images
Figure CN222613303U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture medium adder. Background Art
[0002] During the cell culture process, the culture medium needs to be replaced regularly during routine cell culture. During the cell passaging process, the cell suspension is collected and centrifuged, and then cultured in bottles according to the number of cells. During the bottle culture process, the culture medium needs to be added to multiple culture bottles. In these actual operations, a pipette is often used. The front end of the pipette is connected to the pipette, and the pipette is inserted into the bottle containing the culture medium to aspirate, and then the culture medium is transferred to the culture bottle.
[0003] In the existing cell culture process, it is necessary to use an extractor to extract the cell fluid from the storage container and then transfer it to the culture medium. However, when extracting the cell fluid, it is necessary to open the container before extracting it. The process is easily contaminated by the external environment, which affects the purity and quality of the cells in the culture medium. Based on the shortcomings of the existing technology, the utility model designs a cell culture medium adder. Utility Model Content
[0004] In view of the deficiencies of the prior art, the utility model provides a cell culture medium adder, which has the advantage of being able to complete the transfer of cell fluid without opening the storage container.
[0005] The utility model provides the following technical solutions: a cell culture medium adder, comprising a cell fluid storage box and a pipette dropper, a partition plate is installed inside the cell fluid storage box, a gap exists between the partition plate and the bottom of the inner cavity of the cell fluid storage box, a pipetting structure is installed at the bottom of the outer surface of the partition plate; the pipetting structure comprises two sliding seats and a pipetting port, the pipetting port is arranged on the surface of the partition plate, a slide groove is provided on one side of the two sliding seats, a stopper is slidably installed between the two slide grooves, clamping strips are installed on both sides of the outer surface of the stopper, the two clamping strips are slidably connected to the slide groove, a mounting sleeve is installed on one side of the outer surface of the stopper, a spring is installed inside the mounting sleeve, one end of the spring is fixedly connected to the inner wall of the cell fluid storage box, a clamping slot is provided on one side of the stopper, and an L-shaped opening is provided inside the stopper.
[0006] As a preferred technical solution of the utility model, a water outlet is installed on one side of the outer surface of the cell fluid storage box, and a first sealing plug is placed inside the water outlet.
[0007] As a preferred technical solution of the utility model, a second sealing plug is placed on one side of the top of the cell fluid storage box, and a storage drawer is placed at the bottom of the inner cavity of the cell fluid storage box.
[0008] As a preferred technical solution of the utility model, a protection box is installed on one side of the outer surface of the cell fluid storage box, and a stirring structure is arranged inside the protection box.
[0009] As a preferred technical solution of the utility model, the stirring structure includes a fixing ring, and the fixing ring is fixedly connected to the outer wall of the cell fluid storage box.
[0010] As a preferred technical solution of the utility model, a motor is installed inside the fixing ring, and a rotating rod is installed at one end of the output shaft of the motor.
[0011] As a preferred technical solution of the utility model, three sets of rollers are installed on the outer surface of the rotating rod.
[0012] As a preferred technical solution of the utility model, a plurality of stirring blades are installed on the outer surfaces of the three sets of rollers.
[0013] Compared with the prior art, the utility model has the following beneficial effects:
[0014] 1. A cell culture medium adder, through a liquid transfer structure, when the cell fluid in the cell fluid storage box needs to be transferred to a pipette and added to the culture medium, one end of the pipette is inserted into the card slot, and the pipette is pressed inward, so that one end of the pipette squeezes the block, and the spring is compressed, which can drive the block to slide backward in the slide groove, so that the L-shaped opening can be aligned with the liquid transfer opening, and the cell fluid in the cell fluid storage box can flow into the pipette along the pipette opening and the L-shaped opening, thereby achieving the purpose of completing the transfer of the cell fluid without opening the storage container, and the cell fluid can be quantitatively transferred through the pipette;
[0015] 2. This cell culture medium adder, through the stirring structure, after the cell fluid transfer is completed and the inside of the cell fluid storage box needs to be cleaned, the first sealing plug is inserted into the water outlet, and the second sealing plug is inserted after the cleaning and disinfecting liquid is injected into the cell fluid storage box. At this time, by starting the motor, its output shaft drives the rotating rod to rotate inside the cell fluid storage box, and the cleaning and disinfecting liquid can be fully stirred by multiple stirring leaves to ensure that the inside of the cell fluid storage box is rinsed clean. After rinsing, the first sealing plug is taken out from the water outlet, and after the disinfectant flows out, the cell fluid storage box is air-dried and disinfected by ultraviolet irradiation. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 This is a schematic diagram of the appearance structure of the utility model;
[0017] Figure 2 This is a schematic diagram of the storage drawer structure of the utility model;
[0018] Figure 3This is a schematic diagram of the stirring structure of the utility model;
[0019] Figure 4 This is a schematic diagram of the liquid transfer structure of the utility model;
[0020] Figure 5 This is a schematic diagram of the card slot structure of the utility model.
[0021] In the figure: 1. cell fluid storage box; 2. pipette dropper; 3. water outlet; 4. first sealing plug; 5. second sealing plug; 6. protection box; 7. storage drawer; 8. stirring structure; 81. fixed ring; 82. motor; 83. rotating rod; 84. sleeve roller; 85. stirring leaf; 9. partition plate; 10. pipetting structure; 101. sliding seat; 102. slide groove; 103. block; 104. clamping strip; 105. mounting sleeve; 106. spring; 107. pipetting port; 108. clamping slot; 109. L-shaped port. DETAILED DESCRIPTION
[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0023] See also Figure 1-5 A cell culture medium adder comprises a cell fluid storage box 1 and a pipette dropper 2, wherein a partition plate 9 is installed inside the cell fluid storage box 1, and there is a gap between the partition plate 9 and the bottom of the inner cavity of the cell fluid storage box 1, and a pipetting structure 10 is installed at the bottom of the outer surface of the partition plate 9; the pipetting structure 10 comprises two sliding seats 101 and a pipetting port 107, and the pipetting port 107 is arranged on the surface of the partition plate 9, and a slide groove 102 is provided on one side of the two sliding seats 101, and a stopper 103 is slidably installed between the two slide grooves 102, and clamping strips 104 are installed on both sides of the outer surface of the stopper 103, and the two clamping strips 104 are slidably connected to the slide groove 102, and an installation sleeve 105 is installed on one side of the outer surface of the stopper 103, and a spring 106 is installed inside the installation sleeve 105, and one end of the spring 106 is fixedly connected to the inner wall of the cell fluid storage box 1, and a clamping slot 108 is provided on one side of the stopper 103, and an L-shaped opening 109 is provided inside the stopper 103.
[0024] See also Figure 1-2 A water outlet 3 is installed on one side of the outer surface of the cell fluid storage box 1, and a first sealing plug 4 is placed inside the water outlet 3. A second sealing plug 5 is placed on one side of the top of the cell fluid storage box 1, and a storage drawer 7 is placed at the bottom of the inner cavity of the cell fluid storage box 1.
[0025] The first sealing plug 4 is inserted into the water outlet 3, and the second sealing plug 5 is inserted into the cell fluid storage box 1 to ensure that the cell fluid storage box 1 always maintains a sealed state when there is cell fluid inside. The storage drawer 7 can be used to store some cell culture utensils.
[0026] See also Figure 2-3 A protective box 6 is installed on one side of the outer surface of the cell fluid storage box 1, and a stirring structure 8 is arranged inside the protective box 6. The stirring structure 8 includes a fixed ring 81, and the fixed ring 81 is fixedly connected to the outer wall of the cell fluid storage box 1. A motor 82 is installed inside the fixed ring 81, and a rotating rod 83 is installed at one end of the output shaft of the motor 82. Three sets of rollers 84 are installed on the outer surface of the rotating rod 83. A plurality of stirring paddles 85 are installed on the outer surfaces of the three sets of rollers 84.
[0027] After the cleaning and disinfecting liquid is injected into the cell fluid storage box 1, the second sealing plug 5 is plugged in. At this time, the motor 82 is started, and its output shaft drives the rotating rod 83 to rotate inside the cell fluid storage box 1. The cleaning and disinfecting liquid can be fully stirred by multiple stirring leaves 85 to ensure that the inside of the cell fluid storage box 1 is rinsed clean. After rinsing, the first sealing plug 4 is taken out from the water outlet 3, and after the disinfectant flows out, the cell fluid storage box 1 is air-dried and disinfected by ultraviolet irradiation.
[0028] Working principle, when a cell culture medium adder is used, in the initial state, first, after injecting cell fluid into the cell fluid storage box 1, the second sealing plug 5 blocks the cell fluid storage box 1 and the first sealing plug 4 blocks the water outlet 3. When it is necessary to transfer the cell fluid inside the cell fluid storage box 1 to the pipette 2 and add it to the culture medium, one end of the pipette 2 is inserted into the card slot 108. At this time, the pipette 2 is pressed inward, and one end of the pipette 2 squeezes the stopper 103. After the spring 106 is compressed by force, it can drive the stopper 103 to slide backward in the slide groove 102, thereby allowing the L-shaped opening 109 to align with the pipette port 107. At this time, the cell fluid in the cell fluid storage box 1 can flow along the pipette port 107 and the L-shaped opening 109 flow into the pipette 2, and the cell fluid can be quantitatively transferred through the pipette 2. After the transfer is completed, when the inside of the cell fluid storage box 1 needs to be cleaned, the first sealing plug 4 is inserted into the water outlet 3, and the second sealing plug 5 is plugged in after the cleaning and disinfecting liquid is injected into the cell fluid storage box 1. At this time, by starting the motor 82, its output shaft drives the rotating rod 83 to rotate inside the cell fluid storage box 1, and the cleaning and disinfecting liquid can be fully stirred by multiple stirring leaves 85 to ensure that the inside of the cell fluid storage box 1 is rinsed clean. After rinsing, the first sealing plug 4 is taken out from the water outlet 3, and after the disinfectant flows out, the cell fluid storage box 1 is air-dried and disinfected by ultraviolet irradiation.
[0029] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A cell culture medium additive device, comprising a cell fluid storage box (1) and a pipette (2), characterized in that: A partition plate (9) is installed inside the cell fluid storage box (1), a gap exists between the partition plate (9) and the bottom of the inner cavity of the cell fluid storage box (1), and a liquid transfer structure (10) is installed at the bottom of the outer surface of the partition plate (9); The transfer structure (10) comprises two sliding seats (101) and a transfer port (107), wherein the transfer port (107) is arranged on the surface of the partition plate (9), one side of the two sliding seats (101) is provided with a slide groove (102), a stopper (103) is slidably installed between the two slide grooves (102), both sides of the outer surface of the stopper (103) are provided with clamping strips (104), the two clamping strips (104) are slidably connected to the slide groove (102), one side of the outer surface of the stopper (103) is provided with a mounting sleeve (105), the interior of the mounting sleeve (105) is provided with a spring (106), one end of the spring (106) is fixedly connected to the inner wall of the cell fluid storage box (1), one side of the stopper (103) is provided with a clamping slot (108), and the interior of the stopper (103) is provided with an L-shaped opening (109).
2. A cell culture medium feeder according to claim 1, characterized in that: A water outlet (3) is installed on one side of the outer surface of the cell fluid storage box (1), and a first sealing plug (4) is placed inside the water outlet (3).
3. A cell culture medium feeder according to claim 1, characterized in that: A second sealing plug (5) is placed on one side of the top of the cell fluid storage box (1), and a storage drawer (7) is placed at the bottom of the inner cavity of the cell fluid storage box (1).
4. A cell culture medium feeder according to claim 1, characterized in that: A protection box (6) is installed on one side of the outer surface of the cell fluid storage box (1), and a stirring structure (8) is arranged inside the protection box (6).
5. A cell culture medium feeder according to claim 4, characterized in that: The stirring structure (8) comprises a fixing ring (81), and the fixing ring (81) is fixedly connected to the outer wall of the cell fluid storage box (1).
6. A cell culture medium feeder according to claim 5, characterized in that: A motor (82) is installed inside the fixing ring (81), and a rotating rod (83) is installed at one end of the output shaft of the motor (82).
7. A cell culture medium feeder according to claim 6, characterized in that: Three rollers (84) are installed on the outer surface of the rotating rod (83).
8. A cell culture medium feeder according to claim 7, characterized in that: A plurality of stirring blades (85) are installed on the outer surfaces of the three sleeve rollers (84).