Filtering and separating equipment for stem cells

Abstract

The utility model belongs to the technical field of stem cell filtration, and particularly relates to a filtering and separating device for stem cells, which comprises an outer cylinder and supporting legs, the supporting legs are fixedly connected to four corners of the lower side of the outer cylinder, and the lower end of the inner side of the outer cylinder is fixedly connected with a partition plate. According to the filtering and separating equipment for the stem cells, when the stem cells in cell sap are filtered and separated, a tank cover is opened firstly, then the cell sap to be filtered is poured into a separating tank, then the tank cover is closed, a motor is started, and an output shaft of the motor rotates to drive a driving gear to rotate to enable a driven gear to rotate, so that a liquid discharging pipe is driven to rotate while the separating tank rotates; when stem cells are filtered and separated, an electromagnetic valve in a liquid discharging pipe is opened, and the filtered cell liquid in the separation tank is discharged downwards from the liquid discharging pipe to a discharging hopper at the lower end and then discharged from the lower end of the discharging hopper to be collected.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell filtration, in particular to a filtration separation device for stem cells. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. The stem cell filter separator is a device used to separate stem cells.

[0003] Existing stem cell filtration separators usually include a tank body, an upper filter membrane, a lower filter membrane, a motor and a stirring device. The stirring device is driven by the motor to rotate, and the upper filter membrane and the lower filter membrane are used to separate and filter the cell fluid. However, mechanical stirring can easily cause damage to stem cells and affect the quality of the cells.

[0004] For example, a stem cell filtration separation device disclosed in Chinese patent CN221588518U, when using this device, the cell liquid is injected into the tank body through the liquid inlet, and the motor is started. The motor can drive the upper filter membrane and the lower filter membrane to rotate. The rotation of the upper filter membrane and the lower filter membrane can avoid the clogging of the filter holes due to cell deposition and affect the filtration effect. When the filtration is completed, the waste liquid is discharged from the discharge port, the external threaded column is rotated to separate it from the internal threaded tube, and the column body is removed. The column body no longer blocks the discharge port, and the stem cells can be discharged through the discharge port and the discharge pipe. Then, the tank body is opened to clean other cells on the upper filter membrane, and the stem cell filtration separation can be performed again. Through this device, the filtration separation of stem cells can be completed quickly and conveniently, and when performing filtration separation, the rupture of stem cells caused by stirring can be reduced, and the quality of stem cells after filtration separation can be improved.

[0005] However, after the stem cell filtration separation device filters and discharges the cell fluid, the other cells on the upper filter membrane are cleaned by opening the tank body. However, due to the small size of the other cells on the upper filter membrane, it is difficult to clean the cells by directly cleaning the upper filter membrane in the tank body.

[0006] For this reason, we urgently need to provide a stem cell filtration separation device that is easy to clean the filter membrane. Utility Model Content

[0007] The purpose of the utility model is to provide a filtration separation device for stem cells to solve the problems raised in the above background technology.

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solution: a filtration separation device for stem cells, comprising an outer cylinder and support legs, wherein the support legs are fixedly connected to the four corners of the lower side of the outer cylinder, a partition is fixedly connected to the inner lower end of the outer cylinder, and a filtration mechanism is connected to the interior of the outer cylinder.

[0009] The filtering mechanism includes a separation tank connected to the upper side of the partition, the upper end of the separation tank is hinged with a tank cover by a hinge, the upper inner end of the separation tank is fixedly connected to a fixed annular plate, the front and rear ends of the upper side of the fixed annular plate are fixedly connected to threaded rods, the outer side of the threaded rod is connected to a pressure ring, the upper end of the fixed annular plate is connected to a movable annular plate, the inner side of the movable annular plate is fixedly connected to a filter membrane for filtering stem cells in the cell fluid, a drain pipe is fixedly connected to the middle position of the bottom end of the separation tank, a lower hopper is fixedly connected to the middle position of the bottom end of the outer cylinder, handles are fixedly connected to the left and right ends of the upper side of the movable annular plate, and the inner bottom end of the outer cylinder is connected to a driving unit.

[0010] A further improvement is that the driving unit includes a motor installed on the right side of the bottom end of the inner side of the outer cylinder, the upper end of the output shaft of the motor is fixedly connected to a driving gear, the outer side of the discharge pipe is fixedly connected to a driven gear, rollers are equidistantly arranged on the inner upper end of the outer cylinder, a circular shaft is connected to the inside of the roller, and fixing plates are fixedly connected to the upper and lower ends of the circular shaft.

[0011] A further improvement is that a circular hole is provided at the middle position of the front and rear ends of the movable annular plate, an internal thread is provided on the inner side of the pressure ring, the outer side of the threaded rod is slidably connected to the inner side of the circular hole, the outer side of the threaded rod is threadedly connected to the inner side of the pressure ring, the lower side of the pressure ring abuts against the upper side of the movable annular plate, the outer edge of the movable annular plate is slidably connected to the inner side of the separation tank, and the cross section of the filter membrane is V-shaped. Thus, by opening the tank cover, rotating the pressure ring to remove it from the outer side of the threaded rod, and then lifting the handle, the movable annular plate and the filter membrane can be taken out of the separation tank together.

[0012] A further improvement is that a rubber pad is fixedly connected to the outer edge of the movable annular plate, so that the rubber pad at the outer edge of the movable annular plate contacts the inner side of the separation tank, so that the contact between the movable annular plate and the inner wall of the separation tank can be kept sealed.

[0013] A further improvement is that a rotating hole is opened in the middle position inside the partition, the outer side of the drain pipe is rotatably connected to the inner side of the rotating hole, an electromagnetic valve is arranged inside the drain pipe, and the lower end of the drain pipe is located at the middle position of the upper end of the lower hopper, so that after the cell fluid in the separation tank is filtered and separated, the filtered cell fluid in the separation tank can be discharged downward from the drain pipe to the lower hopper at the lower end by opening the electromagnetic valve in the drain pipe.

[0014] A further improvement is that the lower side of the separation tank is slidably connected to the upper side of the partition, the upper side of the driven gear is slidably connected to the lower side of the partition, and the driving gear and the driven gear are meshed, so that when the output shaft of the starting motor rotates to drive the driving gear to rotate, the driven gear can be driven to rotate, causing the drain pipe to rotate.

[0015] A further improvement is that a rotating hole is opened in the middle position inside the roller, the outer side of the circular shaft is rotatably connected to the inner side of the rotating hole, the upper and lower sides of the roller are slidingly connected to the adjacent sides of the two fixed plates at the upper and lower ends of the circular shaft, the end of the fixed plate away from the circular shaft is fixedly connected to the upper end of the inner side of the outer cylinder body, and the outer side of the roller is rollingly connected to the outer side of the upper end of the separation tank, so that during the rotation of the separation tank, the roller at the upper end of the inner side of the outer cylinder body will be located on the outer side of the upper end of the separation tank and roll.

[0016] In summary, the present application discloses a filtration separation device for stem cells.

[0017] According to the technical solution, after the cell fluid in the separation tank is filtered and discharged, the tank cover is opened, and the pressure ring is rotated to remove it from the outside of the threaded rod, and then the handle is lifted to remove the movable annular plate and the filter membrane from the separation tank together, and then the filter membrane is inverted and rinsed to rinse the cells left by the filtration on the inside of the filter membrane. Therefore, by rinsing the filter membrane in this way of disassembling the filter membrane, it can be ensured that the cells on the inside of the filter membrane can be completely rinsed.

[0018] Furthermore, when the output shaft of the starter motor rotates to drive the driving gear to rotate, the driven gear can be driven to rotate to rotate the discharge pipe, thereby driving the separation tank at the upper end to rotate, and then the cell fluid in the separation tank rotates. At the same time, due to the centrifugal force of the rotation of the cell fluid, the stem cells in the cell fluid can be filtered by the filter membrane more quickly, and the accelerated filtration method can avoid the problem of stem cell rupture caused by mechanical stirring, which can improve the quality of the stem cells after filtration and separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the main structure of the utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of the outer cylinder of the utility model;

[0021] Figure 3 This is a schematic diagram of the structure of the outer end of the filter membrane of the utility model;

[0022] Figure 4 It is a schematic diagram of the structure of the connection between the circular shaft and the roller of the utility model.

[0023] In the figure: 1, outer cylinder; 2, supporting legs; 3, partition; 401, separation tank; 402, tank cover; 403, fixed annular plate; 404, threaded rod; 405, pressure ring; 406, movable annular plate; 407, filter membrane; 408, drain pipe; 409, lower hopper; 410, handle; 501, motor; 502, driving gear; 503, driven gear; 504, roller; 505, round shaft; 506, fixed plate. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] See also Figure 1-Figure 4 The utility model provides a technical solution: a filtration separation device for stem cells, including an outer cylinder 1 and support legs 2, the support legs 2 are fixedly connected to the four corners of the lower side of the outer cylinder 1, the inner lower end of the outer cylinder 1 is fixedly connected with a partition 3, and the interior of the outer cylinder 1 is connected with a filtration mechanism.

[0026] The filtering mechanism includes a separation tank 401 connected to the upper side of the partition 3, the upper end of the separation tank 401 is hinged with a tank cover 402, the upper end of the inner side of the separation tank 401 is fixedly connected with a fixed annular plate 403, the front and rear ends of the upper side of the fixed annular plate 403 are fixedly connected with threaded rods 404, the outer side of the threaded rod 404 is connected with a pressure ring 405, the upper end of the fixed annular plate 403 is connected with a movable annular plate 406, the inner side of the movable annular plate 406 is fixedly connected with a filter membrane 407 for filtering stem cells in the cell fluid, a circular hole is provided at the middle position of the front and rear ends inside the movable annular plate 406, the inner side of the pressure ring 405 is provided with an internal thread, the outer side of the threaded rod 404 is slidably connected to the inner side of the circular hole, the outer side of the threaded rod 404 is threadedly connected to the inner side of the pressure ring 405, and the lower side of the pressure ring 405 is connected to the The upper side of the movable annular plate 406 is abutted, and the outer edge of the movable annular plate 406 is slidably connected with the inner side of the separation tank 401. The cross-section of the filter membrane 407 is V-shaped. Therefore, after the cell fluid in the separation tank 401 is filtered and discharged, the tank cover 402 is opened, and the pressure ring 405 is rotated to remove it from the outer side of the threaded rod 404, and then the handle 410 is lifted to remove the movable annular plate 406 and the filter membrane 407 from the separation tank 401 together, and then the filter membrane 407 is inverted and rinsed to rinse the cells filtered on the inner side of the filter membrane 407. Therefore, by removing the filter membrane 407 in this way, the cells on the inner side of the filter membrane 407 can be completely rinsed, and it is convenient to remove the filter membrane 407 for replacement when it is damaged.

[0027] A rubber pad is fixedly connected to the outer edge of the movable annular plate 406, so that the rubber pad at the outer edge of the movable annular plate 406 contacts the inner side of the separation tank 401, so that the contact point between the movable annular plate 406 and the inner wall of the separation tank 401 can be kept sealed, thereby ensuring that the cell fluid entering the separation tank 401 will be completely filtered by the filter membrane 407.

[0028] A drain pipe 408 is fixedly connected to the middle position of the bottom end of the separation tank 401, a lower hopper 409 is fixedly connected to the middle position of the bottom end of the outer cylinder 1, handles 410 are fixedly connected to the left and right ends of the upper side of the movable annular plate 406, a rotating hole is opened in the middle position of the interior of the partition 3, the outer side of the drain pipe 408 is rotatably connected to the inner side of the rotating hole, a solenoid valve is arranged inside the drain pipe 408, and the lower end of the drain pipe 408 is located at the middle position of the upper end of the lower hopper 409. Therefore, after the cell fluid in the separation tank 401 is filtered and separated, the solenoid valve in the drain pipe 408 is opened, so that the filtered cell fluid in the separation tank 401 can be discharged downward from the drain pipe 408 to the lower hopper 409 at the lower end, and then discharged from the lower end of the lower hopper 409 and collected.

[0029] A driving unit is connected to the inner bottom end of the outer cylinder 1, and the driving unit includes a motor 501 installed on the right side of the inner bottom end of the outer cylinder 1. A driving gear 502 is fixedly connected to the upper end of the output shaft of the motor 501, and a driven gear 503 is fixedly connected to the outer side of the drainage pipe 408. The lower side of the separation tank 401 is slidably connected to the upper side of the partition 3, and the upper side of the driven gear 503 is slidably connected to the lower side of the partition 3. The driving gear 502 and the driven gear 503 are meshed. When the output shaft of the starting motor 501 rotates to drive the driving gear 502 to rotate, the driven gear 503 can be driven to rotate to rotate the drainage pipe 408, thereby driving the separation tank 401 at the upper end to rotate, and then the cell fluid in the separation tank 401 rotates. At the same time, due to the centrifugal force of the rotation of the cell fluid, the stem cells in the cell fluid can be filtered by the filter membrane 407 faster, and the accelerated filtration method can avoid the problem of stem cell rupture caused by mechanical stirring, and can improve the quality of the stem cells after filtration and separation.

[0030] Rollers 504 are equidistantly arranged on the inner upper end of the outer cylinder 1, and a circular shaft 505 is connected to the inside of the roller 504. Fixed plates 506 are fixedly connected to the upper and lower ends of the circular shaft 505. A rotating hole is opened in the middle position inside the roller 504, and the outer side of the circular shaft 505 is rotatably connected to the inner side of the rotating hole. The upper and lower sides of the roller 504 are slidingly connected to the adjacent sides of the two fixed plates 506 at the upper and lower ends of the circular shaft 505. The end of the fixed plate 506 away from the circular shaft 505 is fixedly connected to the inner upper end of the outer cylinder 1, and the outer side of the roller 504 is rollingly connected to the outer side of the upper end of the separation tank 401. Therefore, during the rotation of the separation tank 401, the roller 504 at the inner upper end of the outer cylinder 1 will be located on the outer side of the upper end of the separation tank 401 and roll, thereby limiting the upper end of the outer cylinder 1 so that it can keep rotating stably.

[0031] Working principle: When filtering and separating stem cells in cell fluid, first open the tank cover 402 and pour the cell fluid to be filtered into the separation tank 401, then close the tank cover 402, and then start the motor 501, the output shaft of which rotates to drive the driving gear 502 to rotate, so that the driven gear 503 rotates, thereby driving the drain pipe 408 to rotate and the separation tank 401 to rotate, so that the cell fluid inside the separation tank 401 rotates, and the centrifugal force of the rotation of the cell fluid makes the stem cells in the cell fluid quickly filtered by the filter membrane 407, and the filtration and separation of the stem cells is completed. Open the solenoid valve in the drain pipe 408, and discharge the filtered cell fluid in the separation tank 401 from the drain pipe 408 downward to the lower hopper 409 at the lower end, and then discharge it from the lower end of the lower hopper 409 and be collected. Finally, open the tank cover 402, remove the pressure ring 405 from the outside of the threaded rod 404, and then lift the handle 410 to take out the movable annular plate 406 and the filter membrane 407 from the separation tank 401, then invert the filter membrane 407 and rinse it, rinse off other cells filtered on the inside of the filter membrane 407, and complete the filtration of the cell fluid.

[0032] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "comprise a ..." do not exclude the presence of other identical elements in the process, method, article or device including the elements.

Claims

1. A filtration separation device for stem cells, comprising an outer cylinder (1) and support legs (2), wherein the support legs (2) are fixedly connected to the four corners of the lower side of the outer cylinder (1), characterized in that: A partition plate (3) is fixedly connected to the inner lower end of the outer cylinder (1), and a filtering mechanism is connected to the interior of the outer cylinder (1); The filtering mechanism comprises a separation tank (401) connected to the upper side of the partition (3); the upper end of the separation tank (401) is hingedly connected to a tank cover (402); the upper end of the inner side of the separation tank (401) is fixedly connected to a fixed annular plate (403); the front and rear ends of the upper side of the fixed annular plate (403) are fixedly connected to threaded rods (404); the outer side of the threaded rod (404) is connected to a pressure ring (405); the upper end of the fixed annular plate (403) is connected to a movable annular plate (406); the inner side of the movable annular plate (406) is fixedly connected to a filter membrane (407) for filtering stem cells in a cell fluid; a drain pipe (408) is fixedly connected to the middle position of the bottom end of the separation tank (401); a lower hopper (409) is fixedly connected to the middle position of the bottom end of the outer cylinder (1); handles (410) are fixedly connected to the left and right ends of the upper side of the movable annular plate (406); and a driving unit is connected to the inner bottom end of the outer cylinder (1).

2. A filtration separation device for stem cells according to claim 1, characterized in that: The driving unit comprises a motor (501) mounted on the right side of the bottom end of the inner side of the outer cylinder (1); a driving gear (502) is fixedly connected to the upper end of the output shaft of the motor (501); a driven gear (503) is fixedly connected to the outer side of the discharge pipe (408); rollers (504) are arranged at equal distances on the inner upper end of the outer cylinder (1); a circular shaft (505) is connected to the inside of the roller (504); and fixing plates (506) are fixedly connected to the upper and lower ends of the circular shaft (505).

3. The filtration separation device for stem cells according to claim 1, characterized in that: A circular hole is provided at the middle position of the front and rear ends of the movable annular plate (406), an internal thread is provided on the inner side of the pressure ring (405), the outer side of the threaded rod (404) is slidably connected to the inner side of the circular hole, the outer side of the threaded rod (404) is threadedly connected to the inner side of the pressure ring (405), the lower side of the pressure ring (405) is in contact with the upper side of the movable annular plate (406), the outer edge of the movable annular plate (406) is slidably connected to the inner side of the separation tank (401), and the cross-section of the filter membrane (407) is V-shaped.

4. The filtration separation device for stem cells according to claim 1, characterized in that: A rubber pad is fixedly connected to the outer edge of the movable annular plate (406).

5. The stem cell filtration separation device according to claim 1, characterized in that: A rotating hole is provided in the middle of the interior of the partition (3); the outer side of the drain pipe (408) is rotatably connected to the inner side of the rotating hole; a solenoid valve is provided inside the drain pipe (408); and the lower end of the drain pipe (408) is located at the middle of the upper end of the lower hopper (409).

6. The stem cell filtration separation device according to claim 2, characterized in that: The lower side of the separation tank (401) is slidably connected to the upper side of the partition (3), the upper side of the driven gear (503) is slidably connected to the lower side of the partition (3), and the driving gear (502) and the driven gear (503) are meshed.

7. The stem cell filtration separation device according to claim 2, characterized in that: A rotating hole is provided in the middle of the roller (504); the outer side of the circular shaft (505) is rotatably connected to the inner side of the rotating hole; the upper and lower sides of the roller (504) are slidably connected to the adjacent sides of two fixing plates (506) at the upper and lower ends of the circular shaft (505); one end of the fixing plate (506) away from the circular shaft (505) is fixedly connected to the inner upper end of the outer cylinder (1); and the outer side of the roller (504) is rollingly connected to the outer side of the upper end of the separation tank (401).

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