Detachable protein purification column

Abstract

The utility model discloses a detachable protein purification column which comprises a tank body, a discharge pipe, a filter screen, a filter material, a feed cover and a flow limiting device, the flow limiting device is arranged, protein stock solution is poured into the material guide pipe from the top of the feeding hopper, so that the material guide pipe can flow into the flow limiting box, adjustment is conducted according to requirements, the transmission motor is started to drive the flow limiting plate to rotate, the flow limiting plate can pour the protein stock solution into the flow limiter, and the protein stock solution can flow into the flow limiting box through the flow limiting plate. A flow limiter is matched with a flow limiting plate to change the flow speed of the protein stock solution flowing into the feeding cover, so that the situation that nutrient substances in the protein stock solution are easily lost due to too fast flow is prevented, and the flowing flow speed of the protein stock solution is limited through a flow limiting device; nutrient substances in the protein stock solution cannot easily cause loss due to too fast flowing, so that the problem that the purification rate is reduced due to loss caused by the fact that a large amount of protein stock solution cannot be completely purified due to high pressure and too fast flowing speed of the protein stock solution in the original equipment is solved.

Description

Technical Field

[0001] The utility model relates to the field of biological instruments, in particular to a detachable protein purification column. Background Art

[0002] Protein is an important component of all cells and tissues in the human body. Every tissue in the human body: hair, skin, muscles, bones, internal organs, brain, blood, nerves, endocrine system, etc. are composed of protein; and protein plays a very important role in the life activities of cells and organisms. The structure and properties of organisms are related to protein. Protein is also involved in the regulation of gene expression, redox in cells, electron transfer, neurotransmission and even learning and memory and other life activities.

[0003] A Chinese patent document with publication number CN212631875U discloses a detachable protein purification column, wherein an internal thread is provided at the lower end opening of an upper column, an external thread is provided at the top opening of a lower column, and the internal thread and the external thread cooperate to realize the detachable connection of the upper column and the lower column, thereby realizing the detachable connection of the protein purification column. After use, the protein purification column can be disassembled for easy cleaning;

[0004] However, the protein purification column in the original equipment could not achieve controllable flow rate. When the protein stock solution was first added, a large amount of protein stock solution could not be completely purified and was lost due to the high pressure and fast flow rate of the protein stock solution, resulting in a decrease in the purification rate. When the protein stock solution in the column gradually decreased, the flow rate would be too slow or even stop due to the low pressure of the protein stock solution, further reducing the purification rate. Utility Model Content

[0005] Therefore, in order to solve the above-mentioned shortcomings, the utility model provides a detachable protein purification column.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a detachable protein purification column, comprising a tank body, a discharge pipe, a filter screen, a filter material, a feed cover, and a flow limiting device. The bottom of the tank body is provided with a discharge pipe, the filter screen is fixed to the interior of the tank body, the filter material is installed on the top of the filter screen, the feed cover is threadedly connected to the top of the discharge pipe, the flow limiting device is installed on the top of the feed cover, the flow limiting device comprises a feed hopper, a guide pipe, a flow limiting box, a transmission motor, a flow limiting plate, and a flow limiter. The feed hopper is arranged at the top of the guide pipe, the guide pipe is arranged at the top of the flow limiting box, a transmission motor is installed at the front end of the flow limiting box, the transmission motor is connected to the front end of the flow limiting plate by a coupling, and the flow limiter is installed at the bottom of the flow limiting box.

[0007] As a further solution of the utility model, the flow limiter includes a flow limiter cover 1, a sealing ring, a flow guide tube, a flow limiter cover 2, a flow limiter tube, and a device body. The top of the device body of the flow limiter cover 1 and the bottom of the flow limiter cover 1 are equipped with a sealing ring. The flow guide tube is connected to the bottom of the flow limiter cover 1, the flow limiter cover 2 is fixed to the top of the device body, the flow limiter tube is installed at the bottom of the flow limiter cover 2, and the flow limiter tube is fixed to the inside of the device body.

[0008] As a further solution of the utility model, the feed hopper and the material guiding pipe are vertically arranged on the top of the flow limiting box.

[0009] As a further solution of the utility model, the transmission motor and the current limiting plate are arranged at the front end of the current limiting box at the same level, which is conducive to the transmission motor driving the current limiting plate to rotate.

[0010] As a further solution of the utility model, the flow limiting cover 1 and the flow limiting cover 2 are arranged at the same level at the inner lower end of the flow limiting box.

[0011] As a further solution of the utility model, a leak-proof partition is arranged inside the body.

[0012] As a further solution of the utility model, the flow guide pipe and the flow limiting pipe are arranged in parallel inside the flow limiting box.

[0013] As a further solution of the utility model, the flow limiting tube is a spiral structure as a whole, which can well slow down the flow rate of the material.

[0014] Compared with the prior art, the utility model provides a detachable protein purification column having the following features:

[0015] Beneficial effects:

[0016] 1. The utility model is provided with a flow limiting device, which pours the protein stock solution into the guide pipe from the top of the feed hopper so that the guide pipe can flow into the interior of the flow limiting box, and then adjusts according to demand, and starts the transmission motor to drive the flow limiting plate to rotate, so that the flow limiting plate can pour the protein stock solution into the interior of the flow limiter, and then the flow rate of the protein stock solution flowing into the feed cover is changed by the flow limiter and the flow limiting plate, thereby preventing the nutrients inside the protein stock solution from being easily lost due to excessively fast flow, thereby achieving the goal of limiting the flow rate of the protein stock solution through the flow limiting device, so that the nutrients inside the protein stock solution will not be easily lost due to excessively fast flow, thereby solving the problem in the original equipment that a large amount of protein stock solution cannot be completely purified and is lost due to the high pressure and excessive flow rate of the protein stock solution, resulting in a reduced purification rate.

[0017] 2. A flow limiter is provided in the utility model, and the protein stock solution is poured into the interior of the flow limiting cover 1 and the flow limiting cover 2 respectively through the flow limiting plate, so that the flow limiting cover 2 can pour the protein stock solution into the interior of the flow limiting tube, and the spiral structure of the straight body of the flow limiting tube will delay the flow of the protein stock solution into the interior of the feed cover. In addition, the protein stock solution can be introduced into the interior of the flow limiting tube through the flow limiting cover 1, thereby achieving the goal of changing the flow rate of the protein stock solution flowing into the feed cover through the flow limiter and the flow limiting plate, thereby preventing the problem of nutrients in the protein stock solution being easily lost due to excessively fast flow. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0019] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0020] Figure 3 It is a schematic diagram of the three-dimensional structure of the current limiting device of the utility model;

[0021] Figure 4 It is a schematic diagram of the planar structure of the current limiting device of the utility model;

[0022] Figure 5 It is a structural schematic diagram of the current limiter of the utility model.

[0023] Among them: tank body -1, discharge pipe -2, filter screen -3, filter material -4, feed cover -5, flow limiting device -6, feed hopper -61, guide pipe -62, flow limiting box -63, transmission motor -64, flow limiting plate -65, flow limiter -66, flow limiting cover 1 -661, sealing ring -662, guide pipe -663, flow limiting cover 2 -664, flow limiting pipe -665, device body -666. DETAILED DESCRIPTION

[0024] In order to further explain the technical solution of the present utility model, it is described in detail through specific embodiments below.

[0025] refer to Figure 1 and Figure 2 , in the embodiment of the utility model:

[0026] A detachable protein purification column comprises a tank body 1, a discharge pipe 2, a filter screen 3, a filter material 4, a feed cover 5, and a flow limiting device 6. The discharge pipe 2 is provided at the bottom of the tank body 1, the filter screen 3 is fixed to the interior of the tank body 1, the filter material 4 is installed on the top of the filter screen 3, the feed cover 5 is threadedly connected to the top of the discharge pipe 2, and the flow limiting device 6 is installed on the top of the feed cover 5.

[0027] refer to Figure 3 and Figure 4 , in the embodiment of the utility model:

[0028] The flow limiting device 6 includes a feed hopper 61, a material guide pipe 62, a flow limiting box 63, a transmission motor 64, a flow limiting plate 65, and a flow limiter 66. The feed hopper 61 is arranged at the top of the material guide pipe 62, the material guide pipe 62 is arranged at the top of the flow limiting box 63, and a transmission motor 64 is installed at the front end of the flow limiting box 63. The transmission motor 64 adopts a coupling to drive the front end of the flow limiting plate 65, and the flow limiter 66 is installed at the bottom of the flow limiting box 63. The feed hopper 61 and the material guide pipe 62 are vertically arranged at the top of the flow limiting box 63, and the transmission motor 64 and the flow limiting plate 65 are arranged at the front end of the flow limiting box 63 at the same level, which is conducive to the transmission motor 64 driving the flow limiting plate 65 to rotate.

[0029] refer to Figure 5 , in the embodiment of the utility model:

[0030] The flow limiter 66 includes a flow limiter cover 661, a sealing ring 662, a flow guide pipe 663, a flow limiter cover 664, a flow limiter pipe 665, and a body 666. The top of the body 666 of the flow limiter cover 661 and the bottom of the flow limiter cover 661 are equipped with a sealing ring 662. The flow guide pipe 663 is connected to the bottom of the flow limiter cover 661. The flow limiter cover 664 is fixed to the top of the body 666. The flow limiter pipe 665 is installed at the bottom of the flow limiter cover 664. The flow limiter pipe 665 is fixed to the inside of the body 666. The flow limiter cover 661 and the flow limiter cover 664 are arranged at the same level at the lower end of the inside of the flow limiter box 63. A leak-proof partition is arranged inside the body 666. The flow guide pipe 663 and the flow limiter pipe 665 are arranged in parallel inside the flow limiter box 63. The flow limiter pipe 665 is a spiral structure as a whole, which can well slow down the flow rate of the material.

[0031] refer to Figure 1-Figure 5 When in use, the feed cover 5 is installed on the top of the flow limiting device 6, so that the flow limiting device 6 can play a flow limiting role on the protein stock solution;

[0032] At the same time, pour the protein stock solution from the top of the feed hopper 61 into the guide pipe 62, so that the guide pipe 62 can flow into the interior of the flow limiting box 63, and then adjust according to demand, and start the transmission motor 64 to drive the flow limiting plate 65 to rotate, so that the flow limiting plate 65 can pour the protein stock solution into the interior of the flow restrictor 66;

[0033] The protein stock solution is poured into the interior of the flow limiting cover 1 661 and the flow limiting cover 2 664 respectively through the flow limiting plate 65, so that the flow limiting cover 2 664 can pour the protein stock solution into the interior of the flow limiting tube 665, and the flow of the protein stock solution into the interior of the feed cover 5 is delayed by the spiral structure of the flow limiting tube 665. In addition, the protein stock solution can be introduced into the interior of the flow limiting tube 665 through the flow limiting cover 1 661, and then the flow rate of the protein stock solution flowing into the feed cover 5 is changed by the flow restrictor 66 in cooperation with the flow limiting plate 65, thereby preventing the nutrients inside the protein stock solution from being easily lost due to excessively fast flow, thereby achieving the goal of limiting the flow rate of the protein stock solution through the flow limiting device 6, so that the nutrients inside the protein stock solution will not be easily lost due to excessively fast flow, thereby solving the problem in the original equipment that a large amount of protein stock solution cannot be completely purified and is lost due to the high pressure and too fast flow rate of the protein stock solution, resulting in a reduced purification rate;

[0034] When the protein stock solution flows into the interior of the feed cover 5 from the flow limiting device 6, the protein stock solution can flow into the interior of the tank body 1, so that the protein stock solution can be filtered by the filter material 4, which plays a role of preliminary filtration of the protein stock solution, and the filter net 3 plays a role of secondary filtration of the protein stock solution, thereby ensuring that the product flowing out of the discharge pipe 2 can ensure sufficient purity.

[0035] The control method of the present invention is to control by manually starting and closing the switch. The wiring diagram of the power element and the provision of power supply are common knowledge in the field, and the present invention is mainly used to protect mechanical devices, so the present invention will not explain the control method and wiring arrangement in detail.

[0036] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0037] The above are only preferred examples of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A detachable protein purification column, comprising a tank body (1), a discharge pipe (2), a filter screen (3), a filter material (4), a feed cover (5), and a flow limiting device (6), wherein the bottom of the tank body (1) is provided with a discharge pipe (2), the filter screen (3) is fixed to the inside of the tank body (1), the filter material (4) is installed on the top of the filter screen (3), the feed cover (5) is threadedly connected to the top of the discharge pipe (2), and the flow limiting device (6) is installed on the top of the feed cover (5); Features: The flow limiting device (6) comprises a feed hopper (61), a material guide pipe (62), a flow limiting box (63), a transmission motor (64), a flow limiting plate (65), and a flow limiter (66); the feed hopper (61) is arranged at the top of the material guide pipe (62); the material guide pipe (62) is arranged at the top of the flow limiting box (63); a transmission motor (64) is installed at the front end of the flow limiting box (63); the transmission motor (64) is connected to the front end of the flow limiting plate (65) by a coupling; and the flow limiter (66) is installed at the bottom of the flow limiting box (63).

2. A detachable protein purification column according to claim 1, characterized in that: The flow limiter (66) comprises a flow limiter cover (661), a sealing ring (662), a flow guide tube (663), a flow limiter cover (664), a flow limiter tube (665), and a body (666); the top of the body (666) of the flow limiter cover (661) and the bottom of the flow limiter cover (661) are provided with a sealing ring (662); the flow guide tube (663) is connected to the bottom of the flow limiter cover (661); the flow limiter cover (664) is fixed to the top of the body (666); the bottom of the flow limiter cover (664) is provided with a flow limiter tube (665); and the flow limiter tube (665) is fixed to the inside of the body (666).

3. A detachable protein purification column according to claim 1, characterized in that: The feed hopper (61) and the material guide pipe (62) are vertically arranged on the top of the flow limiting box (63).

4. A detachable protein purification column according to claim 1, characterized in that: The transmission motor (64) and the current limiting plate (65) are arranged at the same level at the front end of the current limiting box (63).

5. A detachable protein purification column according to claim 2, characterized in that: The first flow limiting cover (661) and the second flow limiting cover (664) are arranged at the same level at the lower end of the inner part of the flow limiting box (63).

6. A detachable protein purification column according to claim 2, characterized in that: A leak-proof partition is provided inside the body (666).

7. A detachable protein purification column according to claim 2, characterized in that: The flow guide tube (663) and the flow limiting tube (665) are arranged in parallel inside the flow limiting box (63).

8. A detachable protein purification column according to claim 2, characterized in that: The flow limiting tube (665) is a spiral structure as a whole.

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