Gene sequencing sample library preparation equipment

Abstract

The present disclosure relates to a gene sequencing sample library preparation device, comprising: a consumable box storage device configured to store a consumable box; the automatic pipetting device is configured to perform pipetting treatment on the consumable box and form a sample to be detected in the consumable box; the fluorescent quantitative detection device is configured to perform specific target molecule concentration detection on a sample in the consumable box; and the manipulator is configured to clamp the consumable box in the consumable box storage device, the automatic liquid transfer device and the fluorescent quantitative detection device, and enables the consumable box to be transferred among the consumable box storage device, the automatic liquid transfer device and the fluorescent quantitative detection device.

Description

Technical Field

[0001] The present disclosure relates to the technical field of microbiology instruments, and in particular to equipment for preparing gene sequencing sample libraries. Background Art

[0002] In the process of preparing gene sequencing sample libraries, a variety of different consumables are required. These consumables are usually placed on a workbench, and liquids in the consumables are manually transferred using a pipette to test the samples. When the specifications and quantities of consumables are too many, manual operation is inefficient and prone to errors. Utility Model Content

[0003] The purpose of the present invention is to provide a gene sequencing sample library preparation device to automatically prepare the library and improve the library preparation efficiency and preparation accuracy.

[0004] To achieve the above-mentioned purpose, the present disclosure provides a gene sequencing sample library preparation device, comprising: a consumable box storage device, which is configured to store consumable boxes; an automatic pipetting device, which is configured to perform pipetting on the consumable box and form a sample to be detected in the consumable box; a fluorescence quantitative detection device, which is configured to perform specific target molecule concentration detection on the sample in the consumable box; and a manipulator, which is configured to clamp the consumable box located in the consumable box storage device, the automatic pipetting device and the fluorescence quantitative detection device, and transfer the consumable box among the consumable box storage device, the automatic pipetting device and the fluorescence quantitative detection device.

[0005] According to the gene sequencing sample library preparation equipment disclosed in the present invention, the consumable box is stored in the consumable box storage device. When the consumables in the consumable box need to be used, the manipulator can take out the consumable box from the consumable box storage device and transfer it to the automatic pipetting device. The automatic pipetting device performs pipetting operations on the consumables in the consumable box and forms samples to be tested in the consumable box. The manipulator can transfer the consumable box after pipetting to the fluorescent quantitative detection device. The fluorescent quantitative detection device detects the samples in the consumable box. Thus, the preparation and detection of the library are automatically completed by the gene sequencing sample library preparation equipment, thereby improving the efficiency and accuracy of library preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0006] Figure 1 A top view of a gene sequencing sample library preparation device provided in an embodiment of the present disclosure.

[0007] Figure 2 A schematic diagram of a gene sequencing sample library preparation device after removing the robot arm provided in an embodiment of the present disclosure.

[0008] Figure 3A schematic diagram of a normal temperature storage mechanism provided in an embodiment of the present disclosure.

[0009] Figure 4 A schematic diagram of a normal temperature storage mechanism observed along a direction perpendicular to the vertical axis provided in an embodiment of the present disclosure.

[0010] Figure 5 An exploded view of a support plate and connecting components provided in an embodiment of the present disclosure.

[0011] Figure 6 A schematic diagram of a low temperature storage mechanism provided in accordance with an embodiment of the present disclosure.

[0012] Figure 7 A schematic diagram of a storage station provided for an embodiment of the present disclosure.

[0013] Figure 8 An exploded view of a storage station provided for an embodiment of the present disclosure.

[0014] Fig. 9 A schematic diagram of a cover plate assembly provided in accordance with an embodiment of the present disclosure.

[0015] Fig.10 Schematic diagram of a cover plate and a storage table provided for an embodiment of the present disclosure.

[0016] Fig.11 A schematic diagram of a calibration device provided in an embodiment of the present disclosure.

[0017] Fig.12 A schematic diagram showing a calibration device from one perspective after the outer shell is removed provided in an embodiment of the present disclosure.

[0018] Fig.13 A schematic diagram of another perspective of the calibration device after the outer shell is removed provided in an embodiment of the present disclosure.

[0019] Fig.14 A schematic diagram of a drive mechanism provided in an embodiment of the present disclosure.

[0020] Fig.15 A schematic diagram of a lifting platform, a first cam follower and a first calibration block provided for an embodiment of the present disclosure.

[0021] Fig.16 A schematic diagram of a lifting platform, a second cam follower and a second calibration block provided for an embodiment of the present disclosure.

[0022] Reference numerals:

[0023] 1. Frame;

[0024] 2. Storage device for consumable box; 21. Storage mechanism at normal temperature; 211. First drive assembly; 212. Storage bin; 2121. Support plate; 2122. Support area; 2123. Position-limiting protrusion; 2124. Connecting component; 2125. Upper area; 2126. Lower area; 2127. First connecting part; 2128. Second connecting part; 22. Low-temperature storage mechanism; 221. Box; 222. Storage table; 223. Support seat; 2231. Groove; 2232. Support surface; 2233. Accommodating groove; 2234. Hole; 224. Adapter for consumable box; 225. Semiconductor cooling sheet; 226. Radiator; 227. Cover assembly; 2271. Second drive assembly; 2272. Second motor; 2273. Screw assembly; 2274. Slide; 2275. Cover; 228. Condensate collecting tank;

[0025] 3. Automatic pipetting device; 31. Inlet and outlet of consumable box; 32. Transmission shaft;

[0026] 4. Fluorescence quantitative detection device;

[0027] 5. Robotic arm;

[0028] 6. Consumables box;

[0029] 7. Calibration device; 71. Housing; 711. Placement surface; 712. First fixed block; 713. Second fixed block; 714. First slot; 715. Second slot; 72. First calibration block; 73. Second calibration block; 74. First mounting seat; 741. First slider; 742. First guide rail; 743. First cam follower; 75. Second mounting seat; 751. Second slider; 752. Second guide rail; 753. Second cam follower; 76. Driving mechanism; 761. Fixed seat; 762. Third driving assembly; 763. Lifting platform; 7631. First inclined plane; 7632. Second inclined plane; 764. Third guide rail; 77. First elastic element; 78. Second elastic element;

[0030] 8. Film sealing device;

[0031] L, vertical axis; M, spacing; X, first direction; Y, second direction. DETAILED DESCRIPTION

[0032] The technical solution of the present disclosure is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the present disclosure, rather than to limit the present disclosure. It should also be noted that, for ease of description, only the parts related to the present disclosure are shown in the accompanying drawings, rather than all of them.

[0033] Some directional words are defined in the present disclosure. Unless otherwise stated, the directional words used, such as "up", "down", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of the present disclosure.

[0034] In the present disclosure, unless otherwise expressly specified and limited, a first feature being “above” or “below” a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being “above”, “above”, and “above” a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0035] In the description of the present disclosure, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.

[0036] Overview :

[0037] Figure 1 A top view of a gene sequencing sample library preparation device is shown. Figure 2 FIG. 1 shows a schematic diagram of a gene sequencing sample library preparation device after removing the robot arm. Figure 1 and Figure 2 As shown, the gene sequencing sample library preparation device can be used for gene sequencing sample library preparation, but is not limited thereto. The gene sequencing sample library preparation device includes a rack 1 and a consumable box storage device 2, an automatic liquid transfer device 3, a fluorescence quantitative detection device 4 and a manipulator 5 installed on the rack 1.

[0038] The consumable box storage device 2 is configured to store consumable boxes 6. The consumable box 6 is used to accommodate consumables. Specifically, the upper surface of the consumable box 6 generally has a plurality of holes arranged regularly (e.g., arranged in a matrix), and the consumables (e.g., reagents, samples, etc.) are accommodated in the holes. Figure 1As shown, the shape of the consumable box 6 can be rectangular. The consumable box 6 can have a variety of different specifications. For example, the consumable box 6 can have different numbers of holes, such as 96 holes, 384 holes, etc. The consumable box 6 can also have different thicknesses so that the holes have different depths. The consumable box 6 is, for example, a deep well plate.

[0039] like Figure 2 As shown, in order to save the area of ​​the rack 1 while storing a large number of consumable boxes 6, the consumable box storage device 2 can have multiple layers, and the consumable boxes 6 can be arranged in the multiple layers, so that a large number of consumable boxes 6 can be stacked in a limited area.

[0040] like Figure 1 and Figure 2 As shown, in this embodiment, the consumable box storage device 2 includes a normal temperature storage mechanism 21 and a low temperature storage mechanism 22. In the normal temperature storage mechanism 21, the consumable box 6 is at normal temperature, that is, the ambient temperature of the gene sequencing sample library preparation device. In the low temperature storage mechanism 22, the consumable box 6 is at a low temperature, such as 4°C, so as to store certain reagents and samples that must be stored at low temperatures.

[0041] The automatic pipetting device 3 is configured to perform pipetting on the consumable box 6 and form a sample to be detected in the consumable box 6. Specifically, Figure 1 and Figure 2 As shown, the automatic pipetting device 3 has a consumable box inlet and outlet 31, and a transmission shaft 32 is arranged on the consumable box inlet and outlet 31. The end of the transmission shaft 32 extends from the consumable box inlet and outlet 31, so that the consumable box 6 can be taken and placed on the transmission shaft 32. The transmission shaft 32 is used to send the consumable box 6 into the automatic pipetting device 3 or send the consumable box 6 out of the automatic pipetting device 3, and the transmission shaft 32 has a positioning function to position the consumable box 6 at a preset position. For example, when the transmission shaft 32 sends the consumable box 6 out of the automatic pipetting device 3, the position of the consumable box 6 is fixed, so that the manipulator 5 can clamp the consumable box 6. The automatic pipetting device 3 has a pipette gun (not shown) or a similar component with a pipetting function inside to complete the pipetting operation.

[0042] It should be noted that the structure and control method of the automatic liquid transfer device 3 are both in the prior art. As an example, the automatic liquid transfer device 3 can be an automatic liquid transfer workstation in the prior art.

[0043] The fluorescence quantitative detection device 4 is configured to detect the concentration of specific target molecules in the sample in the consumable box 6. It should be noted that the structure and control method of the fluorescence quantitative detection device 4 belong to the prior art. As an example, the fluorescence quantitative detection device 4 can be a fluorescence quantitative instrument.

[0044] The manipulator 5 is configured to clamp the consumable box 6 located in the consumable box storage device 2, the automatic pipetting device 3 and the fluorescence quantitative detection device 4, and transfer the consumable box 6 between the consumable box storage device 2, the automatic pipetting device 3 and the fluorescence quantitative detection device 4. It should be noted that the relevant structure and control method of the manipulator 5 belong to the prior art. As an example, the manipulator 5 can be a four-axis manipulator or a six-axis manipulator so that the manipulator 5 has sufficient degrees of freedom.

[0045] like Figure 1 As shown, the consumable box storage device 2, the automatic pipetting device 3 and the fluorescence quantitative detection device 4 can be arranged in sequence around the manipulator 5 in a counterclockwise direction so that the manipulator 5 has a relatively short motion trajectory. It should be noted that the positions of the consumable box storage device 2, the automatic pipetting device 3, the fluorescence quantitative detection device 4 and the manipulator 5 on the rack 1 can be adjusted according to actual needs.

[0046] like Figure 1 and Figure 2 As shown, both the normal temperature storage mechanism 21 and the low temperature storage mechanism 22 face the robot 5 so that the robot 5 can conveniently take and place the consumable box 6 .

[0047] like Figure 1 and Figure 2 As shown, the gene sequencing sample library preparation device may further include a calibration device 7, which is configured to calibrate the position of the consumable box 6, especially before placing the consumable box 6 on the conveying shaft 32 and before placing the consumable box 6 on the fluorescence quantitative detection device 4. Generally, the calibration device 7 can be arranged near the conveying shaft 32 and the fluorescence quantitative detection device 4, for example, Figure 1 and Figure 2 In the embodiment, the calibration device 7 is arranged between the conveying axis 32 and the fluorescence quantitative detection device 4.

[0048] like Figure 1 and Figure 2 As shown, the gene sequencing sample library preparation device may further include a sealing device 8, which is configured to seal the sample after detection. The sealing device 8 may be arranged on one side of the fluorescence quantitative detection device 4. Figure 1 In the embodiment, the film sealing device 8 is arranged above the fluorescence quantitative detection device 4, so that the consumable box 6 can be transferred to the film sealing device 8 via a shorter path after being detected by the fluorescence quantitative detection device 4. It should be noted that the structure and control method of the film sealing device 8 belong to the prior art and will not be described in detail here.

[0049] Normal temperature storage mechanism :

[0050] Figure 3 Schematic diagram of the normal temperature storage mechanism 21 is shown. Figure 3As shown, the normal temperature storage mechanism 21 includes a first drive component 211 and a storage bin 212. The first drive component 211 is located at the bottom of the storage bin 212 and is fixedly mounted on the frame 1. The storage bin 212 is roughly cylindrical and rotatably mounted on the frame 1. The first drive component 211 is used to drive the storage bin 212 to rotate around the vertical axis L. As the storage bin 212 rotates, the position of the consumable box 6 in the storage bin 212 can change relative to the frame 1 and the manipulator 5, so as to manually load materials into the storage bin 212 and the manipulator 5 can clamp the required consumable box 6 from the storage bin 212.

[0051] As an example, the first driving assembly 211 may include a first motor and a transmission component that converts the rotational motion of the first motor into the rotational motion of the storage bin 212. The transmission component may include a plurality of gears meshing with each other, but is not limited thereto.

[0052] like Figure 3 As shown, the storage bin 212 includes a plurality of support plates 2121 spaced apart along a vertical axis L, and each support plate 2121 has a plurality of support areas 2122 arranged (especially evenly arranged) around the vertical axis L, for example Figure 3 Each support plate 2121 has eight support areas 2122. The consumable box 6 can be placed in the support area 2122. Generally, each support area 2122 allows one consumable box 6 to be placed. In addition, each support plate 2121 is aligned with each other, so that the support areas 2122 on different support plates 2121 are aligned with each other. Figure 3 The arrangement direction of the support areas 2122 on different support plates 2121 is parallel to the vertical axis L.

[0053] like Figure 3 As shown, the edge of the support area 2122 has a plurality of limiting protrusions 2123, so that when the consumable box 6 is placed in the support area 2122, the position of the consumable box 6 is relatively fixed and will not be shifted due to the rotation of the storage bin 212. For simplicity, the limiting protrusions 2123 are only shown in some support areas 2122 in this embodiment.

[0054] It should be noted that, in this embodiment, the support plate 2121 is an independent, complete plate. When the support plate 2121 is manufactured, the relative positions of the various support areas 2122 on the support plate 2121 are determined within the tolerance range, ensuring the position accuracy between the various support areas 2122. It is only necessary to control the rotation angle of the storage bin 212 to make the required support area 2122 face the manipulator 5 or the manual loading position. In addition, since the various support plates 2121 are aligned with each other, the support areas 2122 at the same position on each support plate 2121 can accurately face the manipulator 5 or the manual loading position at the same time, which is particularly convenient for the manipulator 5 to take and place the consumable box 6 from the support area 2122.

[0055] Figure 4 FIG. 2 shows a schematic diagram of the normal temperature storage mechanism 21 observed along a direction perpendicular to the vertical axis L. Figure 4 As shown, the storage bin 212 also includes a connecting component 2124, and the two upper and lower adjacent support plates 2121 are connected by the connecting component 2124. As an example, the connecting component 2124 can be a connecting rod, and the upper end of the connecting rod is connected to the bottom surface of one support plate 2121, and the lower end of the connecting rod is connected to the top surface of another support plate 2121, so that the upper and lower support plates 2121 are arranged at intervals.

[0056] The distance M between the upper and lower support plates 2121 may be determined by the connecting component 2124 . When the length of the connecting component 2124 increases, the distance M between the upper and lower support plates 2121 increases accordingly.

[0057] In this embodiment, along the direction of the vertical axis L, the above-mentioned spacing M is not constant, but changes, for example, Figure 4 In FIG. 2 , it can be clearly observed that the spacing M located in the upper region 2125 of the storage bin 212 is greater than the spacing M located in the lower region 2126 of the storage bin 212. Figure 4 In the embodiment, those consumable boxes 6 with thicker thickness can be placed in the upper area 2125, and those consumable boxes 6 with thinner thickness can be placed in the lower area 2126. By reasonably setting the spacing M, the height of the storage bin 212 can be appropriately reduced to reduce the height occupied by the gene sequencing sample library preparation device.

[0058] It should be noted that the setting of the above-mentioned spacing M is only exemplary. In other embodiments, the spacing M in the upper area 2125 may be smaller than the spacing M in the lower area 2126, or the spacing M may monotonically increase or decrease from bottom to top along the direction of the vertical axis L.

[0059] Since the distance M in this embodiment is not constant, the installation method between the connecting component 2124 and the supporting plate 2121 is described below.

[0060] Figure 5 An exploded view of the connecting member 2124 and the supporting plate 2121 is shown. Figure 5 As shown, in this embodiment, each support plate 2121 has the same shape and has a first connection portion 2127 and a second connection portion 2128, wherein the first connection portion 2127 and the second connection portion 2128 are two different positions on the surface of the support plate 2121, and the first connection portion 2127 and the second connection portion 2128 both penetrate the upper and lower surfaces of the support plate 2121. Figure 5 Taking the support plate 2121 located in the middle as an example, the connecting part 2124 located on the upper surface of the support plate 2121 is connected to the first connecting part 2127, and the connecting part 2124 located on the lower surface of the support plate 2121 is connected to the second connecting part 2128. Therefore, the upper and lower connecting parts 2124 are respectively on an axis, rather than on the same axis, that is, the upper and lower connecting parts 2124 are staggered with each other.

[0061] Two adjacent support plates 2121 may be independently connected using one or more connection components 2124. When connection components 2124 of different lengths are used, the distance M between the support plates 2121 changes accordingly.

[0062] Compared with installing all the support plates 2121 on the same longer connecting rod, the installation method of this embodiment can omit the device for limiting each support plate 2121, and the structure is simpler.

[0063] As an example, Figure 5 As shown, in this embodiment, the first connection part 2127 and the second connection part 2128 are both mounting holes. For example, the support plate 2121 is provided with 12 mounting holes evenly arranged around the vertical axis L. The mounting holes with odd numbers can be used as the first connection part 2127, and the mounting holes with even numbers can be used as the second connection part 2128, that is, a second connection part 2128 is set between two adjacent first connection parts 2127, and correspondingly, a first connection part 2127 is also set between two adjacent second connection parts 2128. As a result, the connecting part 2124 above the support plate 2121 and the connecting part 2124 below the support plate 2121 are offset by 30°.

[0064] Low temperature storage mechanism :

[0065] Figure 6 Schematic diagram of the low temperature storage mechanism 22 is shown. Figure 6As shown, the low-temperature storage mechanism 22 includes a box body 221 and a plurality of storage tables 222 installed in the box body 221. The front side of the box body 221 is open, and the front side of the box body 221 faces the manipulator 5 so that the manipulator 5 extends from the front side of the box body 221 into the interior of the box body 221. The plurality of storage tables 222 are spaced apart along the height direction of the box body 221. Each storage table 222 can accommodate at least one consumable box 6. Each storage table 222 has a refrigeration function so that the consumable box 6 placed on the storage table 222 can be kept at a low temperature.

[0066] Figure 7 A schematic diagram of one of the storage stations 222 is shown. Figure 8 An exploded view of the storage station 222 is shown. Figure 7 and Figure 8 As shown, the storage table 222 includes a support base 223, and the upper surface of the support base 223 has a groove 2231. The shape of the groove 2231 is slightly larger than the consumable box adapter 224. The groove 2231 is used to accommodate the consumable box adapter 224. The consumable box 6 can be detachably placed on the consumable box adapter 224. The bottom surface of the groove 2231 is the support surface 2232. When the consumable box adapter 224 is installed in the groove 2231, the bottom surface of the consumable adapter 224 is in contact with the support surface 2232.

[0067] like Figure 8 As shown, a receiving groove 2233 is provided on the support surface 2232, and the semiconductor cooling sheet 225 is located in the receiving groove 2233, and the cooling surface (upper surface) of the semiconductor cooling sheet 225 is flush with the support surface 2232. Therefore, when the consumable box 224 is installed in the groove 2231, the bottom surface of the consumable box adapter 224 is in direct contact with the cooling surface of the semiconductor cooling sheet 225, and the consumable box adapter 224 can be cooled by the semiconductor cooling sheet 225. When the consumable box 6 is placed on the consumable box adapter 224, the consumable box 6 is cooled accordingly.

[0068] like Figure 8 As shown, the storage station 222 also includes a heat sink 226, which is in contact with the heating surface (lower surface) of the semiconductor cooling plate 225 and is used to cool the heating surface to ensure the normal operation of the semiconductor cooling plate 225.

[0069] It should be noted that the consumable box adapter 224 and the semiconductor cooling sheet 225 are both related to the prior art and will not be described in detail here.

[0070] like Figure 8 As shown, in this embodiment, the support base 223 has two grooves 2231, so each support base 223 can hold at most two consumable boxes 6. However, in other embodiments, the number of grooves 2231 on the support base 223 can be adjusted as needed.

[0071] like Figure 8 As shown, in this embodiment, the receiving groove 2233 accommodates two semiconductor cooling chips 225 placed side by side. However, in other embodiments, the number of semiconductor cooling chips 225 can be adjusted accordingly according to the size of the consumable box adapter 224, so that the semiconductor cooling chips 225 can be in contact with most of the bottom surface of the consumable box adapter 224 to ensure the cooling of the consumable box adapter 224.

[0072] In addition, temperature sensors (not shown) are respectively installed on the cooling surface and the heating surface of the semiconductor refrigeration plate 225 to detect the temperature of the cooling surface and the heating surface of the semiconductor refrigeration plate 225. On the one hand, it is ensured that the cooling temperature generated by the cooling surface of the semiconductor refrigeration plate 225 meets the requirements, and on the other hand, it is ensured that the temperature of the heating surface of the semiconductor refrigeration plate 225 does not exceed the relevant requirements, thereby ensuring the normal operation of the semiconductor refrigeration plate 225.

[0073] like Figure 8 As shown, in this embodiment, the bottom of the accommodating groove 2233 is exposed on the bottom surface of the support seat 223, the heat sink 226 is located on the bottom surface of the support seat 223, and the upper surface of the heat sink 226 is in direct contact with the heating surface of the semiconductor cooling plate 225 for dissipating heat from the heating surface.

[0074] As an example of the radiator 226, the radiator 226 may be a water cooling row, that is, water cooling may be used to dissipate heat from the heating surface of the semiconductor cooling plate 225. However, in other embodiments, other heat dissipation methods may also be used, such as air cooling.

[0075] Fig. 9 A schematic diagram of the cover plate assembly 227 is shown. Fig.10 A schematic diagram of the cover plate 2275 and the storage station 222 is shown. Fig. 9 and Fig.10 As shown, the low-temperature storage mechanism 22 also includes a cover assembly 227, which is used to cover the upper part of the consumable box 6 to seal the upper part of the consumable box 6 to avoid condensation water from forming on the upper part of the consumable box 6. The cover assembly 227 includes a second drive assembly 2271 and a cover 2275. The cover 2275 is located above the support seat 223 and is installed on the second drive assembly 2271. The second drive assembly 2271 drives the cover 2275 to move up and down. When the cover 2275 moves downward, the lower surface of the cover 2275 can abut against the top surface of the consumable box 6, thereby closing the top surface of the consumable box 6, so that the consumables in the consumable box 6 are isolated, preventing condensed water in the low-temperature storage mechanism 22 from flowing into the consumables; when the cover 2275 moves upward, a gap is formed between the cover 2275 and the consumable box 6, so as to take the consumable box 6 out of the low-temperature storage mechanism 22 or put the consumable box 6 into the low-temperature storage mechanism 22.

[0076] As an example, the second drive assembly 2271 includes a second motor 2272, a screw component 2273 and a slide 2274, the power input end of the screw component 2273 is connected to the second motor 2272, the power output end of the screw component 2273 is connected to the slide 2274, the slide 2274 is configured to be movable up and down, and the cover 2275 is installed on the slide 2274, thereby, the screw component 2273 converts the rotational motion of the second motor 2272 into the up and down movement of the slide 2274, thereby realizing the up and down movement of the cover 2275.

[0077] In this embodiment, there are multiple cover plates 2275, and the multiple cover plates 2275 are installed on the slide 2274 at intervals up and down. When the slide 2274 moves up and down, the multiple cover plates 2275 move up and down at the same time so as to contact or separate from the consumable boxes 6 on the multiple storage tables 222 at the same time.

[0078] like Figure 8 As shown, during the use of the low-temperature storage mechanism 22, a small amount of condensed water will be generated on the surface of the consumable box 6. In order to collect the condensed water, a plurality of holes 2234 are further provided at the edge of the support surface 2232. A condensed water collecting groove 228 is provided below the holes 2234. Therefore, the condensed water on the surface of the consumable box 6 can drip into the condensed water collecting groove 228 through the holes 2234. The condensed water collecting groove 228 is installed on the support seat 223. Both the condensed water collecting groove 228 and the support seat 223 are installed on the inner wall of the box body 221.

[0079] Calibration device :

[0080] Fig.11 FIG. 8 is a schematic diagram of a calibration device 7. Fig.11 As shown, the calibration device 7 includes a shell 71, which is roughly a rectangular parallelepiped. The top surface of the shell 71 is a placement surface 711. A placement area for placing the consumable box 6 is defined above the placement surface 711. A first fixed block 712 is provided on the first side of the placement area in the first direction X, and a first calibration block 72 is provided on the second side of the placement area in the first direction X. The first calibration block 72 can move along the first direction X. When the consumable box 6 is placed in the placement area, the first calibration block 72 can push the consumable box 6 to the position of the first fixed block 712 and abut against the first fixed block 712 to realize the calibration of the consumable box 6 in the first direction X. In addition, a second fixed block 713 is provided on the first side of the second direction Y of the placement area, and a second calibration block 73 is provided on the second side of the first direction X of the placement area. The second calibration block 73 can be movable along the second direction Y. When the consumable box 6 is placed in the placement area, the second calibration block 73 can push the consumable box 6 to the position of the second fixed block 713 and abut against the second fixed block 713 to realize the calibration of the consumable box 6 in the second direction Y.

[0081] It should be noted that, in this embodiment, the first direction X and the second direction Y are perpendicular to each other, the second side of the first direction X is arranged opposite to the first side of the first direction X, and the second side of the second direction Y is arranged opposite to the first side of the second direction Y.

[0082] By moving the first calibration block 72 and the second calibration block 73, the consumable box 6 can be positioned in the space defined by the first fixed block 712, the second fixed block 713, the first calibration block 72 and the second calibration block 73, so that the position of the consumable box 6 is calibrated, so that the manipulator 5 can accurately grasp the calibrated consumable box 6.

[0083] like Fig.11 As shown, in this embodiment, the first fixing block 712 and the second fixing block 713 are fixedly arranged on the placement surface 711, especially at the edge of the placement surface 711. The number of the first fixing block 712 and the second fixing block 713 can be multiple, for example Fig.11 There are two first fixing blocks 712 and two second fixing blocks 713. The first fixing block 712 and the second fixing block 713 have guiding slopes on the sides facing the placement area, so that when the consumable box 6 is placed in the placement area, the guiding slopes can guide the consumable box 6 to be placed on the placement surface 711.

[0084] like Fig.11 As shown, the shapes of the first calibration block 72 and the second calibration block 73 can be cylindrical or other shapes.

[0085] like Fig.11 As shown, a first slot 714 and a second slot 715 are provided on the placement surface 711, the first calibration block 72 is inserted into the first slot 714, and the second calibration block 73 is inserted into the second slot 715. The shapes of the first slot 714 and the second slot 715 can be long strips, wherein the first slot 714 extends along the first direction X, and the second slot 715 extends along the second direction Y, so that the first calibration block 72 extends along the first direction X, and the second calibration block 73 extends along the second direction Y.

[0086] like Fig.11 As shown, in this embodiment, there are two first calibration blocks 72 and two second calibration blocks 73, and correspondingly, there are two first slots 714 and two second slots 715. In other embodiments, the number of the first calibration blocks 72 and the second calibration blocks 73 can be adjusted as needed.

[0087] In this embodiment, the two first calibration blocks 72 move simultaneously, and the two second calibration blocks 73 move simultaneously.

[0088] The first calibration block 72 and the second calibration block 73 can be driven independently or in conjunction with each other by a driving mechanism 76 installed inside the housing 71 .

[0089] In this embodiment, the driving mechanism 76 mentioned later drives the first calibration block 72 and the second calibration block 73 to approach or move away from the placement area at the same time, that is, the first calibration block 72 and the second calibration block 73 are linked to each other.

[0090] Fig.12 and Fig.13 Schematic diagrams of the calibration device 7 after the outer shell 71 is removed are shown respectively. Fig.12 and Fig.13 As shown, the calibration device 7 also includes a first mounting seat 74, two first calibration blocks 72 are fixedly mounted on the first mounting seat 74, the first mounting seat 74 is fixedly connected to the first slider 741, the first slider 741 is slidably connected to the first guide rail 742, the first guide rail 742 is fixedly mounted on the housing 71, and the first guide rail 742 extends along the first direction X, whereby, when the first mounting seat 74 slides on the first guide rail 742, the two first calibration blocks 72 move along the first direction X.

[0091] Similar, such as Fig.12 and Fig.13 As shown, the calibration device 7 also includes a second mounting seat 75, two second calibration blocks 73 are fixedly mounted on the second mounting seat 75, the second mounting seat 75 is fixedly connected to the second slider 751, the second slider 751 is slidably connected to the second guide rail 752, the second guide rail 752 is fixedly mounted on the housing 71, and the second guide rail 752 extends along the second direction Y, so that when the second mounting seat 75 slides on the second guide rail 752, the two second calibration blocks 73 move along the second direction Y.

[0092] like Fig.13 As shown, the first mounting seat 74 is also connected to a first cam follower 743, and the second mounting seat 75 is also connected to a second cam follower 753. The first cam follower 743 and the second cam follower 753 are used to connect with a driving mechanism 76. The driving mechanism 76 drives the first cam follower 743 and the second cam follower 753 to move, thereby driving the first mounting seat 74 and the second mounting seat 75 to move.

[0093] Fig.14 Schematic diagram of the drive mechanism 76 is shown. Fig.13 and Fig.14 As shown, the driving mechanism 76 includes a fixed seat 761, a third driving assembly 762 and a lifting platform 763. The fixed seat 761 is L-shaped ( Fig.14 Only the bottom of the fixing seat 761 is shown in FIG. 1 ), the fixing seat 761 is fixedly mounted on the housing 71, and the lifting platform 763 is connected to the third guide rail 764 (see FIG. 1 ). Fig.15 ) can be slidably mounted on the fixed seat 761, and the third drive assembly 762 is fixedly mounted on the lifting platform 763. The third drive assembly 762 has a power output end ( Fig.14 The power output end is connected to the fixed seat 761. When the third driving assembly 762 moves, the lifting platform 763 is driven to rise and fall relative to the fixed seat 761.

[0094] like Fig.13 and Fig.14 As shown, the lifting platform 763 includes a first inclined surface 7631 and a second inclined surface 7632 . The first inclined surface 7631 faces the first direction X and is connected to the first calibration block 72 , and the second inclined surface 7632 faces the second direction Y and is connected to the second calibration block 73 .

[0095] Fig.15 FIG. 7 shows a schematic diagram of the first inclined surface 7631 and the first cam follower 743. Fig.15 As shown, at this time, the lifting platform 763 is in a low position, and the first cam follower 743 is on the upper part of the first inclined surface 7631, so that the first calibration block 72 is in a position relatively close to the placement area, which is defined as the calibration position of the first calibration block 72. As the lifting platform 763 gradually moves upward, the first inclined surface 7631 will push the first cam follower 743 to gradually move to the right, so that the first calibration block 72 is away from the placement area, and the first calibration block 72 moves from the calibration position to the non-calibration position.

[0096] Fig.16 FIG. 7 shows a schematic diagram of the second inclined surface 7632 and the second cam follower 753. Fig.16 As shown, the lifting platform 763 is at a low position at this time, and the second cam follower 753 is at the upper part of the second inclined surface 7632, so that the second calibration block 73 is at a position relatively close to the placement area, which is defined as the calibration position of the second calibration block 73. As the lifting platform 763 gradually moves upward, the second inclined surface 7632 will push the second cam follower 753 to gradually move to the left, so that the second calibration block 73 is away from the placement area, and the second calibration block 73 moves from the calibration position to the non-calibration position.

[0097] That is to say, when the lifting platform 763 is in a low position, the first calibration block 72 is connected to the upper part of the first inclined surface 7631, and the second calibration block 73 is connected to the upper part of the second inclined surface 7632, and the first calibration block 72 and the second calibration block 73 are both located in a calibration position relatively close to the placement area; when the lifting platform 763 is in a high position, the first calibration block 72 is connected to the lower part of the first inclined surface 7631, and the second calibration block 73 is connected to the lower part of the second inclined surface 7632, and the first calibration block 72 and the second calibration block 73 are both located in a non-calibration position relatively far away from the placement area.

[0098] like Fig.15 As shown, in order to ensure that the first cam follower 743 can always abut against the first inclined surface 7631, a first elastic element 77 is connected to the first mounting seat 74, and the first elastic element 77 applies a force toward the first inclined surface 7631 to the first mounting seat 74, so that the first cam follower 743 abuts against the first inclined surface 7631.

[0099] Similar, such as Fig.16 As shown, in order to ensure that the second cam follower 753 can always abut against the second inclined surface 7632, a second elastic element 78 is connected to the second mounting seat 75, and the second elastic element 78 applies a force toward the second inclined surface 7632 to the second mounting seat 75, so that the second cam follower 753 abuts against the second inclined surface 7632.

[0100] The use process of the calibration device 7 is as follows:

[0101] When it is necessary to calibrate the position of the consumable box 6, the third driving component 762 drives the lifting platform 763 to move upward, so that the first inclined surface 7631 and the second inclined surface 7632 both move upward. Under the action of the first inclined surface 7631, the first calibration block 72 is away from the placement area, and under the action of the second inclined surface 7632, the second calibration block 73 is away from the placement area. At this time, the manipulator 5 can easily place the consumable box 6 in the placement area. After the consumable box 6 is placed on the placement surface 711 (a sensor can be used to detect whether the consumable box 6 is placed on the placement surface 711), the third driving component 762 drives the lifting platform 763 to move downward. At this time, the first calibration block 72 moves toward the placement area under the action of the first elastic element 77, and the second calibration block 73 moves toward the placement area under the action of the second elastic element 78. Then, the consumable box 6 is pushed toward the first fixed block 712 by the first calibration block 72, and is pushed toward the second fixed block 713 by the second calibration block 73, thereby realizing the calibration of the position of the consumable box 6.

[0102] How to use :

[0103] The working process of the gene sequencing sample library preparation equipment is as follows:

[0104] Step 1: manually placing the consumable box 6 in the consumable box storage device 2;

[0105] Step 2: The manipulator 5 grabs the required consumable box 6 in the consumable box storage device 2, and places the consumable box 6 on the calibration device 7. The calibration device 7 calibrates the position of the consumable box 6. Then, the manipulator 5 places the calibrated consumable box 6 on the conveying shaft 32. The conveying shaft 32 conveys the consumable box 6 to the automatic liquid transfer device 3. The automatic liquid transfer device 3 transfers the consumable box 6. The consumable box 6 after transfer is output through the conveying shaft 32.

[0106] Step 3: For the consumable box 6 that can continue to be used, the manipulator 5 clamps the consumable box 6 and places it on the calibration device 7 for position calibration, and then the manipulator 5 puts the calibrated consumable box 6 back into the consumable box storage device 2; for the consumable box 6 with a sample, the manipulator 5 clamps the consumable box 6 and places it on the calibration device 7 for position calibration, and then the manipulator 5 places the calibrated consumable box 6 on the fluorescence quantitative detection device 4;

[0107] Step 4: The fluorescence quantitative detection device 4 detects the concentration of specific target molecules on the sample in the consumable box 6;

[0108] Step 5: the robot 5 clamps the consumable box 6 in the fluorescence quantitative detection device 4 and places it in the sealing device 8. The sealing device 8 seals the consumable box 6, and the robot 5 puts the sealed consumable box 6 back into the consumable box storage device 2.

[0109] The above is a description of the embodiments of the present disclosure. Through the above description of the disclosed embodiments, professionals and technicians in the field can implement or use the present disclosure. Various modifications to these embodiments will be apparent to professionals and technicians in the field. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novelties disclosed herein.

Claims

1. A gene sequencing sample library preparation device, characterized in that: The gene sequencing sample library preparation device comprises: A consumable box storage device (2), wherein the consumable box storage device (2) is configured to store consumable boxes (6); An automatic liquid transfer device (3), the automatic liquid transfer device (3) being configured to perform liquid transfer processing on the consumable box (6) and form a sample to be detected in the consumable box (6); A fluorescence quantitative detection device (4), wherein the fluorescence quantitative detection device (4) is configured to detect the concentration of target molecules in the sample in the consumable box (6); and A manipulator (5) is configured to clamp the consumable box (6) located in the consumable box storage device (2), the automatic pipetting device (3) and the fluorescent quantitative detection device (4), and transfer the consumable box (6) between the consumable box storage device (2), the automatic pipetting device (3) and the fluorescent quantitative detection device (4).

2. The gene sequencing sample library preparation device according to claim 1, characterized in that: The consumables box storage device (2) comprises: A normal temperature storage mechanism (21), wherein the normal temperature storage mechanism (21) comprises: A storage bin (212), the storage bin (212) being rotatable about a vertical axis (L), the storage bin (212) comprising: A support plate (2121), the support plate (2121) having a plurality of support areas (2122) arranged around the vertical axis (L), the support plate (2121) having a plurality of support plates (2121), the plurality of support plates (2121) being arranged at intervals up and down along the vertical axis (L), and the support areas (2122) of the plurality of support plates (2121) being aligned with each other; and A connecting component (2124), wherein two upper and lower adjacent support plates (2121) are connected via the connecting component (2124).

3. The gene sequencing sample library preparation device according to claim 2, characterized in that: a. The storage bin (212) comprises an upper region (2125) and a lower region (2126), the length of the connecting member (2124) in the upper region (2125) being different from the length of the connecting member (2124) in the lower region (2126); and / or b. Along the vertical axis (L) from bottom to top, the length of the connecting component (2124) increases or decreases monotonically.

4. The gene sequencing sample library preparation device according to claim 2, characterized in that: The support plate (2121) includes a first connecting portion (2127) and a second connecting portion (2128); the connecting component (2124) located above the support plate (2121) is connected to the first connecting portion (2127), and the connecting component (2124) located below the support plate (2121) is connected to the second connecting portion (2128), so that the connecting component (2124) above the support plate (2121) and the connecting component (2124) below the support plate (2121) are on different axes.

5. The gene sequencing sample library preparation device according to claim 1, characterized in that: The consumables box storage device (2) comprises: A low temperature storage mechanism (22), wherein the low temperature storage mechanism (22) comprises: A support seat (223), the support seat (223) having a support surface (2232), and a receiving groove (2233) is provided on the support surface (2232); a semiconductor refrigeration sheet (225), the semiconductor refrigeration sheet (225) being located in the containing groove (2233), and the cooling surface of the semiconductor refrigeration sheet (225) being flush with the supporting surface (2232); and A heat sink (226), wherein the heat sink (226) is in contact with the heating surface of the semiconductor cooling plate (225).

6. The gene sequencing sample library preparation device according to claim 5, characterized in that: The bottom of the accommodating groove (2233) is exposed on the bottom surface of the supporting seat (223), and the heat sink (226) is located on the bottom surface of the supporting seat (223); and / or The radiator (226) is a water-cooled radiator.

7. The gene sequencing sample library preparation device according to claim 5, characterized in that: The low temperature storage mechanism (22) further comprises: A cover plate assembly (227), the cover plate assembly (227) comprising: a second drive assembly (2271); and A cover plate (2275), wherein the cover plate (2275) is located above the support seat (223), and the cover plate (2275) is installed on the second driving component (2271), and the second driving component (2271) drives the cover plate (2275) to move up and down.

8. The gene sequencing sample library preparation device according to claim 5, characterized in that: A plurality of holes (2234) are also provided at the edge of the support surface (2232), and the low-temperature storage mechanism (22) further comprises: A condensed water collecting groove (228), wherein the condensed water collecting groove (228) is located below the hole (2234) so ​​as to collect condensed water dripping from the hole (2234).

9. The gene sequencing sample library preparation device according to claim 1, characterized in that: The gene sequencing sample library preparation device also includes: A calibration device (7), wherein the calibration device (7) is configured to calibrate the position of the consumable box (6), and the calibration device (7) comprises: A placement surface (711), wherein an upper portion of the placement surface (711) defines a placement area; A first fixing block (712), the first fixing block (712) being located on a first side of the placement area in a first direction (X); a first calibration block (72), the first calibration block (72) being located at a second side of the placement area in a first direction (X), the first calibration block (72) being configured to be movable along the first direction (X), wherein the second side in the first direction (X) is arranged opposite to the first side in the first direction (X); a second fixing block (713), the second fixing block (713) being located at a first side of a second direction (Y) of the placement area, wherein the first direction (X) and the second direction (Y) are perpendicular to each other; and A second calibration block (73), the second calibration block (73) is located on a second side of a second direction (Y) of the placement area, the second calibration block (73) is configured to be movable along the second direction (Y), wherein the second side of the second direction (Y) is arranged opposite to the first side of the second direction (Y).

10. The gene sequencing sample library preparation device according to claim 9, characterized in that: The calibration device (7) further comprises: A driving mechanism (76), wherein the driving mechanism (76) is configured to drive the first calibration block (72) and the second calibration block (73) to move closer to or farther from the placement area at the same time, and the driving mechanism (76) comprises: A lifting platform (763), wherein the lifting platform (763) comprises: a first inclined surface (7631), the first inclined surface (7631) faces the first direction (X), and the first inclined surface (7631) is connected to the first calibration block (72); and a second inclined surface (7632), the second inclined surface (7632) facing the second direction (Y), and the second inclined surface (7632) being connected to the second calibration block (73); Wherein, the lifting platform (763) has a high position and a low position; Wherein, when the lifting platform (763) is located at the low position, the first calibration block (72) is connected to the upper part of the first inclined surface (7631), and the second calibration block (73) is connected to the upper part of the second inclined surface (7632), and the first calibration block (72) and the second calibration block (73) are both located at calibration positions relatively close to the placement area; Wherein, when the lifting platform (763) is in the high position, the first calibration block (72) is connected to the lower part of the first inclined surface (7631), and the second calibration block (73) is connected to the lower part of the second inclined surface (7632), and the first calibration block (72) and the second calibration block (73) are both located in a non-calibration position relatively away from the placement area.

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