PCR amplification temperature control device

Abstract

The embodiment of the utility model provides a PCR amplification temperature control device. The PCR amplification temperature control device comprises a heat dissipation base; the plurality of heating sources are arranged at the top of the heat dissipation base; the heating plate is arranged on the heating sources, and the plurality of heating sources divide the heating plate into at least two areas; the PCR reaction plate is matched with the heating plate; the heat preservation heat cover mechanism is connected with the heat dissipation base through a steering engine, and the steering engine drives the heat preservation heat cover mechanism to rotate, so that the heat preservation heat cover mechanism makes contact with the heat dissipation base to cover the heating source or is separated from the heat dissipation base. The heating plate is at least divided into two areas through the multiple heating sources, the multiple heating sources can heat the different areas of the heating plate, zoned temperature control is achieved, temperature transition is smooth, the whole heating plate is adopted, the temperature consistency during PCR amplification is good, the heat preservation heat cover mechanism is arranged, and the heat preservation effect is good. The temperature consistency during PCR amplification can be well ensured, and meanwhile liquid evaporation is prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of PCR amplification temperature control, and in particular to a PCR amplification temperature control device. Background Art

[0002] PCR is an enzymatic chemical reaction. During the PCR amplification process, temperature needs to be controlled. Existing PCR temperature control generally keeps the entire 96-well plate at the same temperature or controls the temperature in three zones. However, when the temperature is controlled in three zones, the temperature transition is not smooth, and it is difficult to achieve uniform temperature transition. Summary of the invention

[0003] In view of this, an embodiment of the present specification provides a PCR amplification temperature control device, which divides the heating plate into at least two areas through multiple heat sources. The multiple heat sources can heat different areas of the heating plate to achieve zoned temperature control, and the temperature transition is relatively smooth. By using a whole heating plate, the temperature consistency during PCR amplification is better.

[0004] The embodiments of this specification provide the following technical solutions: a PCR amplification temperature control device, comprising:

[0005] Heat dissipation base;

[0006] A plurality of heat sources are arranged on the top of the heat dissipation base;

[0007] A heating plate disposed on the heat source, wherein the plurality of heat sources divide the heating plate into at least two areas;

[0008] A PCR reaction plate matched with the heating plate;

[0009] The heat-insulating heat cover mechanism is connected to the heat-dissipating base through a steering gear, and the steering gear drives the heat-insulating heat cover mechanism to rotate, so that the heat-insulating heat cover mechanism contacts the heat-dissipating base to cover the heat source or is separated from the heat-dissipating base.

[0010] Preferably, the heat-insulating cover mechanism includes a cover plate, a connecting arm, a heat insulation plate, a silicone rubber heating sheet, a fixing plate and a heat insulation film; the cover plate and the connecting arm are integrally formed; the cover plate is connected to the servo engine via the connecting arm; the heat insulation plate, the silicone rubber heating sheet, the fixing plate and the heat insulation film are arranged in sequence in a direction away from the cover plate.

[0011] Preferably, the heat-insulating cover mechanism further comprises an adjusting pad, wherein the adjusting pad is arranged between the heat-insulating plate and the cover plate, and the adjusting pad is used to adjust the position of the heat-insulating film so that the heat-insulating film fits tightly with the PCR reaction plate.

[0012] Preferably, the device further comprises a plurality of temperature control components, and the plurality of temperature control components respectively control the connection between the plurality of heat sources and the silicone rubber heating sheet.

[0013] Preferably, the device further comprises a PCR insulation plate, and the PCR insulation plate is arranged along the periphery of the heating plate.

[0014] Preferably, the device further comprises a plurality of first temperature sensors, and the plurality of first temperature sensors are respectively installed in different areas at least divided into which the heating plate is divided by the heat source;

[0015] The device further comprises a plurality of second temperature sensors, and the plurality of second temperature sensors are respectively and evenly installed in the heat preservation cover mechanism.

[0016] Preferably, the heat source comprises a semiconductor refrigeration sheet, and both sides of the semiconductor refrigeration sheet are coated with heat dissipation silicone grease.

[0017] Preferably, the heat dissipation base includes two groups of plate-shaped heat sinks, the two groups of plate-shaped heat sinks are connected via a connecting plate, and the heat source is installed on the top of the connecting plate.

[0018] Preferably, the heat dissipation base further includes two groups of heat dissipation fans, and the two groups of heat dissipation fans are respectively installed under the two groups of sheet-like radiators.

[0019] Compared with the prior art, the at least one technical solution adopted in the embodiments of this specification can achieve the following beneficial effects:

[0020] The heating plate is divided into at least two areas by multiple heat sources. The multiple heat sources can heat different areas of the heating plate to achieve zoned temperature control and a smoother temperature transition. By using a whole heating plate, the temperature consistency during PCR amplification is better. By setting a heat-insulating hot cover mechanism, the temperature consistency during PCR amplification can be better ensured, while preventing the evaporation of the liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0022] Figure 1 It is a structural schematic diagram of the PCR amplification temperature control device provided by the utility model;

[0023] Figure 2 It is a structural schematic diagram of a heating plate of a PCR amplification temperature control device provided by the utility model;

[0024] Figure 3 The utility model is a schematic diagram of the internal structure of the cover plate of the PCR amplification temperature control device. DETAILED DESCRIPTION

[0025] The embodiments of the present application are described in detail below with reference to the accompanying drawings.

[0026] The following describes the implementation methods of the present application through specific examples, and those skilled in the art can easily understand other advantages and effects of the present application from the contents disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The present application can also be implemented or applied through other different specific implementation methods, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that, in the absence of conflict, the following embodiments and the features in the embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work belong to the scope of protection of the present application.

[0027] It should be noted that various aspects of the embodiments within the scope of the appended claims are described below. It should be apparent that the aspects described herein can be embodied in a wide variety of forms, and any specific structure and / or function described herein is merely illustrative. Based on the present application, it should be understood by those skilled in the art that an aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspect described herein can be used to implement the device and / or practice the method. In addition, other structures and / or functionalities other than one or more of the aspects described herein can be used to implement this device and / or practice this method.

[0028] It should also be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. The drawings only show components related to the present application rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0029] Additionally, in the following description, specific details are provided to facilitate a thorough understanding of the examples. However, it will be understood by those skilled in the art that the aspects described may be practiced without these specific details.

[0030] The technical solutions provided by various embodiments of the present application are described below in conjunction with the accompanying drawings.

[0031] like Figure 1-Figure 2As shown, a PCR amplification temperature control device comprises: a heat dissipation base, a plurality of heat sources 10, a heating plate 9, a PCR reaction plate 4 and a heat preservation cover mechanism;

[0032] A plurality of heat sources 10 are arranged on the top of the heat dissipation base. When the PCR reaction needs to be cooled, the heat dissipation base can be used for cooling. The heating plate 9 is arranged on the top of the heat source 10. The plurality of heat sources 10 divide the heating plate 9 into at least two areas. By dividing the heating plate 9 into different areas through the plurality of heat sources 10, both the same temperature control of the heating plate 9 and the temperature gradient control of the heating plate 9 can be achieved. The heating plate 9 is a whole plate, which can ensure the temperature consistency during PCR amplification. The PCR reaction plate 4 cooperates with the heating plate 9. The heat source 10 heats the heating plate 9, and the heating plate 9 transfers the heat to the PCR reaction plate 4 to heat the PCR reaction plate 4; the heat-insulating heat cover mechanism is connected to the heat dissipation base through the steering gear 5, and the steering gear 5 drives the heat-insulating heat cover mechanism to rotate, so that the heat-insulating heat cover mechanism contacts the heat dissipation base to cover the heat source 10 or is separated from the heat dissipation base. The heat-insulating heat cover mechanism is driven to rotate by the steering gear 5. When the PCR amplification reaction is performed, the heat-insulating heat cover mechanism can better ensure the temperature consistency during PCR amplification and prevent the evaporation of the liquid.

[0033] The heating plate 9 is divided into at least two areas by multiple heat sources 10. The multiple heat sources 10 can heat different areas of the heating plate 9 to achieve zoned temperature control and a smoother temperature transition. By using a whole heating plate 9, the temperature consistency during PCR amplification is better. By setting a heat-insulating hot cover mechanism, the temperature consistency during PCR amplification can be better ensured, while preventing the evaporation of the liquid.

[0034] It should be noted that, in this embodiment, six groups of heat sources 10 are provided, and the six groups of heat sources 10 divide the heating plate 9 into three areas; in other embodiments, the heat sources 10 can also be other numbers, such as four groups, eight groups, etc., to divide the heating plate 9 into two, four, etc. areas, which can be selected according to actual conditions.

[0035] It should also be noted that the heating plate 9 is a plate with 96 holes; the PCR reaction plate 4 can adopt medical standard parts, which will not be elaborated in detail here. The PCR reaction plate 4 is inserted into the heating plate 9 and fits tightly to increase the heat conduction area and facilitate heat transfer.

[0036] like Figure 3As shown, in some embodiments, the heat-insulating heat cover mechanism includes a cover plate 7, a connecting arm 6, a heat insulation plate 11, a silicone rubber heating sheet 12, a fixing plate 13 and a heat insulation film 8. The cover plate 7 and the connecting arm 6 are integrally formed, and the cover plate 7 is connected to the steering gear 5 through the connecting arm 6. The heat insulation plate 11, the silicone rubber heating sheet 12, the fixing plate 13 and the heat insulation film 8 are arranged in sequence along the direction away from the cover plate 7. The steering gear 5 can drive the cover plate 7 to rotate through the connecting arm 6, and the heat insulation plate 11, the silicone rubber heating sheet 12, the fixing plate 13 and the heat insulation film 8 move with the cover plate 7. When the cover plate 7 is covered on the heat dissipation base, the heat insulation film 8 is tightly fitted with the PCR reaction plate 4, which can prevent the evaporation of the liquid during PCR amplification from affecting the concentration. The heat insulation is performed by arranging the heat insulation film 8 and the heat insulation plate 11, and the heat insulation effect is good, which can avoid heat dissipation. Heat is generated by the rubber heating sheet to ensure temperature consistency.

[0037] It should be noted that the cover plate 7 can be made of a material mixed with glass fiber and silicate adhesive to ensure the heat insulation effect.

[0038] like Figure 3 As shown, further, the heat-insulating heat cover mechanism also includes an adjusting pad 14, and the adjusting pad 14 is arranged between the heat insulation plate 11 and the cover plate 7. The adjusting pad 14 is used to adjust the position of the heat insulation film 8 so that the heat insulation film 8 and the PCR reaction plate 4 are tightly fitted. By setting the adjusting pad 14, the thickness of the adjusting pad 14 can be adjusted, thereby adjusting the position of the heat insulation film 8 to ensure that when the cover plate 7 is covered on the heat dissipation base, the heat insulation film 8 and the PCR reaction plate 4 are tightly fitted.

[0039] In some embodiments, the device further comprises a plurality of temperature control components, wherein the plurality of temperature control components respectively control and connect the plurality of heat sources and the silicone rubber heating sheet 12. By setting a plurality of temperature control components to respectively control the plurality of heat sources 10, the temperature of different areas can be controlled, thereby achieving zoned temperature control. The temperature of different areas can be the same temperature, or can be increased or decreased according to demand. The temperature of the silicone rubber heating sheet 12 is controlled by the temperature control components, thereby achieving consistency between the temperature inside the thermal insulation cover mechanism and the temperature of the heating plate 9.

[0040] like Figure 1 As shown, in some embodiments, the device also includes a PCR insulation plate 3, and the PCR insulation plate 3 is arranged along the periphery of the heating plate 9. By arranging the PCR insulation plate 3 around the heating plate 9, the PCR insulation plate 3 can play a heat preservation effect, avoid heat dissipation of the heating plate 9, and ensure the heating effect of the PCR reaction plate 4.

[0041] It should be noted that the PCR insulation board 3 can be made of a material mixed with glass fiber and silicate adhesive to ensure the insulation effect.

[0042] It should also be noted that the PCR insulation plate 3 and the cover plate 7 can be made of the same insulation material. By setting a larger gap and using air for insulation, the temperature consistency during PCR amplification can be ensured as much as possible while preventing the evaporation of the liquid.

[0043] In some embodiments, the device further comprises a plurality of first temperature sensors, which are respectively installed in different areas into which the heating plate 9 is at least divided by the heat source 10. By arranging the first temperature sensors in different areas of the heating plate 9, the first temperature sensors monitor the temperatures of the different areas in real time.

[0044] The device also includes a plurality of second temperature sensors, which are evenly installed in the thermal insulation cover mechanism. By arranging the second temperature sensors in the thermal insulation cover mechanism, the second temperature sensors monitor the temperature in the thermal insulation cover mechanism in real time.

[0045] In some embodiments, the heat source 10 includes a semiconductor refrigeration sheet, and both sides of the semiconductor refrigeration sheet are coated with heat dissipation silicone grease. Using the semiconductor refrigeration sheet as the heat source 10 has high heating efficiency and is convenient for rapid heating of the heating plate 9. At the same time, heat dissipation silicone grease is coated on both sides of the semiconductor refrigeration sheet to facilitate the transfer of heat generated by the semiconductor refrigeration sheet to the heating plate 9.

[0046] like Figure 1 As shown, in some embodiments, the heat dissipation base includes two groups of sheet-like radiators 1, and the two groups of sheet-like radiators 1 are connected by a connecting plate 2. The heat source 10 is installed on the top of the connecting plate 2. By setting two groups of sheet-like radiators 1 and the connecting plate 2, the heat source 10 is installed on the connecting plate 2, and the sheet-like radiator 1 can achieve the effect of heat dissipation.

[0047] like Figure 1 As shown, in some embodiments, the heat dissipation base also includes two groups of heat dissipation fans 15, and the two groups of heat dissipation fans 15 are respectively installed under the two groups of sheet-like heat sinks 1. By installing the heat dissipation fans 15 under the two groups of sheet-like heat sinks 1, when the PCR reaction needs to be cooled, the heat dissipation fans 15 can be started to ensure rapid cooling.

[0048] See also Figure 1-Figure 3 , the specific working process of the PCR amplification temperature control device will be described below:

[0049] When in use, the PCR reaction plate 4 is installed on the heating plate 9, and the PCR reaction plate 4 is inserted into the heating plate 9, tightly fitting, increasing the heat conduction area and facilitating heat transfer. Reaction liquid is added to the PCR reaction plate 4, and the steering gear 5 drives the cover plate 7 to rotate through the connecting arm 6, and the heat insulation plate 11, the silicone rubber heating plate 12, the fixing plate 13 and the heat insulation film 8 move with the cover plate 7, so that the cover plate 7 is covered on the heat dissipation base, and the heat insulation film 8 is tightly fitted to the PCR reaction plate 4. According to heating needs, multiple semiconductor refrigeration sheets are controlled to work respectively through multiple first temperature control components, and multiple semiconductor refrigeration sheets heat different areas of the heating plate 9, thereby realizing zoned temperature control. The temperatures of different areas can be the same or different according to needs. Increasing or decreasing, the heating plate 9 transfers heat to the reaction plate 4 to achieve heating of the reaction liquid. The temperature of the silicone rubber heating plate 12 is controlled by the second temperature control component, so as to achieve consistency between the internal temperature of the thermal insulation cover mechanism and the temperature of the heating plate 9. When performing the PCR amplification reaction, the thermal insulation film 8 fits tightly against the PCR reaction plate 4 to prevent the evaporation of the liquid during PCR amplification from affecting the concentration. The thermal insulation film 8 and the thermal insulation plate 11 can provide thermal insulation with good thermal insulation effect to avoid heat dissipation. The PCR insulation plate 3 can achieve the effect of thermal insulation to avoid heat dissipation of the heating plate 9 and ensure the heating effect of the PCR reaction plate 4. When the reaction is completed and cooling is required, the cooling fan 15 can be started to ensure rapid cooling.

[0050] The same or similar parts of the various embodiments in this specification can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the method embodiment described later, since it corresponds to the system, the description is relatively simple, and the relevant parts can be referred to the partial description of the system embodiment.

[0051] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A PCR amplification temperature control device, characterized in that: include: Heat dissipation base; A plurality of heat sources are arranged on the top of the heat dissipation base; A heating plate disposed on the heat source, wherein the plurality of heat sources divide the heating plate into at least two areas; A PCR reaction plate matched with the heating plate; The heat-insulating heat cover mechanism is connected to the heat-dissipating base through a steering gear, and the steering gear drives the heat-insulating heat cover mechanism to rotate, so that the heat-insulating heat cover mechanism contacts the heat-dissipating base to cover the heat source or is separated from the heat-dissipating base.

2. The PCR amplification temperature control device according to claim 1, characterized in that: The heat-insulating cover mechanism comprises a cover plate, a connecting arm, a heat-insulating plate, a silicone rubber heating sheet, a fixing plate and a heat-insulating film. The cover plate and the connecting arm are integrally formed. The cover plate is connected to the steering gear via the connecting arm. The heat-insulating plate, the silicone rubber heating sheet, the fixing plate and the heat-insulating film are sequentially arranged in a direction away from the cover plate.

3. The PCR amplification temperature control device according to claim 2, characterized in that: The heat-insulating cover mechanism further comprises an adjusting pad, which is arranged between the heat-insulating plate and the cover plate, and is used to adjust the position of the heat-insulating film so that the heat-insulating film is closely fitted to the PCR reaction plate.

4. The PCR amplification temperature control device according to claim 2, characterized in that: The device also includes a plurality of temperature control components, and the plurality of temperature control components respectively control the connection between the plurality of heat sources and the silicone rubber heating sheet.

5. The PCR amplification temperature control device according to claim 1, characterized in that: The device also includes a PCR insulation plate, which is arranged along the periphery of the heating plate.

6. The PCR amplification temperature control device according to claim 1, characterized in that: The device further comprises a plurality of first temperature sensors, wherein the plurality of first temperature sensors are respectively installed in different areas at least divided into different areas by the heat source; And / or, the device further comprises a plurality of second temperature sensors, and the plurality of second temperature sensors are respectively and evenly installed in the heat-insulating thermal cover mechanism.

7. The PCR amplification temperature control device according to claim 1, characterized in that: The heat source comprises a semiconductor refrigeration sheet, and both sides of the semiconductor refrigeration sheet are coated with heat dissipation silicone grease.

8. The PCR amplification temperature control device according to claim 1, characterized in that: The heat dissipation base comprises two groups of plate-shaped heat sinks, the two groups of plate-shaped heat sinks are connected via a connecting plate, and the heat source is installed on the top of the connecting plate.

9. The PCR amplification temperature control device according to claim 8, characterized in that: The heat dissipation base also includes two groups of heat dissipation fans, and the two groups of heat dissipation fans are respectively installed under the two groups of sheet-like heat sinks.

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