Multi-stage filtering device for preparing stem cells

Abstract

The utility model relates to the technical field of medical instruments, and discloses a multistage filtering device for preparing stem cells, which comprises a cleaning barrel, the cleaning barrel is composed of a barrel body, a cavity, a barrel edge and a drain pipe, a shell cover is mounted at the top of the barrel body, a driving mechanism is mounted in the barrel body, and the cavity is communicated with the shell cover. A cavity is formed in the base, a first filtering mechanism, a second filtering mechanism and a third filtering mechanism are placed in the cavity and detachably connected, the first filtering mechanism is connected with the driving mechanism in a clamped mode, the first filtering mechanism comprises a first shell, the first shell is located in the cavity, and the second filtering mechanism comprises a second shell which is located in the cavity. A filter screen I is fixedly connected to the middle part of the shell I, and a plurality of groups of liquid outlets are formed in one side wall of the shell. The liquid is uniformly dispersed through centrifugal force, blockage is avoided, the filtering efficiency is improved, meanwhile, the detachable design is adopted, cells are conveniently collected, filter screens with different pore diameters are conveniently replaced, and diversified requirements are met.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a multi-stage filtering device for preparing stem cells. Background Art

[0002] In the prior art, the preparation of stem cells usually requires multiple steps of separation and purification, among which cell filtration is one of the key steps. The commonly used filtration method currently mainly adopts static filtration, that is, gravity or simple pressure difference is used to push the cell suspension through a filter membrane or screen with a specific pore size, thereby achieving the separation of cells or impurities of different sizes. This static filtration method has been widely used in basic research and preliminary cell preparation work due to its simple operation and low cost.

[0003] However, during the filtration process, cells, tissue fragments and impurities tend to accumulate on the surface of the filter membrane, gradually covering the effective filtration pore size, resulting in reduced flux or even complete blockage. Once blockage occurs, the flow rate of the filtered liquid will be significantly slowed down, and the operator will have to stop operating to clean the filter device or replace the filter screen, which not only increases the workload but also leads to the loss of target cells. Utility Model Content

[0004] In order to make up for the above shortcomings, the utility model provides a multi-stage filtration device for preparing stem cells, aiming to improve the problem that the static filtration method in the prior art is prone to cause clogging of the filter membrane, reduced flux, operation interruption and loss of target cells, affecting the separation efficiency and workflow.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A multi-stage filtering device for preparing stem cells, comprising a cleaning barrel, the cleaning barrel consisting of a barrel body, a chamber, a barrel edge and a liquid discharge pipe, a shell cover installed on the top of the barrel body, a driving mechanism installed inside the barrel body, a first filtering mechanism, a second filtering mechanism and a third filtering mechanism placed inside the chamber, the first filtering mechanism, the second filtering mechanism and the third filtering mechanism being detachably connected, and the first filtering mechanism and the driving mechanism being snap-connected;

[0007] The first filtering mechanism comprises a shell 1, the shell 1 is located inside the chamber, a filter screen 1 is fixedly connected to the middle of the shell 1, and a side wall of the shell is provided with a plurality of drainage ports;

[0008] As a further description of the above technical solution:

[0009] The second filtering mechanism comprises a second housing, the second housing is threadedly connected to the top of the first housing, and a second filter is fixedly connected to the middle of the second housing;

[0010] As a further description of the above technical solution:

[0011] The third filtering mechanism comprises a shell three, the shell three is threadedly connected to the top of the shell two, a filter net three is fixedly connected to the middle of the shell three, a clamp ring is fixedly connected to the top of the shell three, and the clamp ring is overlapped on the inner side of the barrel edge;

[0012] As a further description of the above technical solution:

[0013] The driving mechanism comprises a motor, the motor is fixedly connected to the inside of the barrel, a magnetic rotor is fixedly connected to the output end of the motor, a copper rotor is placed at the bottom of the chamber, a slot is provided at the bottom of the shell, and the copper rotor is engaged with the slot;

[0014] As a further description of the above technical solution:

[0015] A feeding mechanism is installed on the top of the shell cover, and the feeding mechanism includes a feeding hopper and a valve. The feeding hopper is fixedly connected to the top of the shell cover, the bottom of the feeding hopper passes through the shell cover and is connected to the inside of the cleaning barrel, and the valve is installed in the middle of the feeding hopper;

[0016] As a further description of the above technical solution:

[0017] A flushing mechanism is installed outside the barrel body, and the flushing mechanism includes a water pump and a connecting hose. The water pump is fixedly connected to the outside of the barrel body, and the connecting hose is fixedly connected to the output end of the water pump. The other end of the connecting hose passes through the shell cover and is connected to the cleaning barrel.

[0018] As a further description of the above technical solution:

[0019] The inner bottom of the housing is provided with a protrusion, and the copper rotor is located inside the protrusion;

[0020] As a further description of the above technical solution:

[0021] One end of the connecting hose away from the water pump is rotatably connected to the middle part of the shell cover.

[0022] The utility model has the following beneficial effects:

[0023] 1. In the utility model, the copper rotor is driven to rotate by the magnetic rotor, so that the entire multi-stage filtering mechanism generates centrifugal force during the filtering process. The liquid is evenly dispersed on the surface of the filter screen under the action of gravity and centrifugation, avoiding the blockage problem caused by concentrated entry at a single point, thereby greatly improving the filtering efficiency and the operating stability of the device;

[0024] 2. In the present invention, the first filter mechanism, the second filter mechanism and the third filter mechanism are detachable, which is convenient for users to collect stem cells from the filter net and can freely replace the filter nets with different pore sizes according to needs to adapt to stem cell separation and preparation tasks with different specifications and precision requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a three-dimensional schematic diagram of a multi-stage filtration device for preparing stem cells proposed by the utility model;

[0026] Figure 2 This is a cross-sectional schematic diagram of a cleaning barrel of a multi-stage filtration device for preparing stem cells proposed in the utility model;

[0027] Figure 3 This is a schematic diagram of the structure explosion of a filtering mechanism of a multi-stage filtering device for preparing stem cells proposed by the utility model;

[0028] Figure 4 This is a schematic structural diagram of a driving mechanism of a multi-stage filtration device for preparing stem cells proposed in the utility model.

[0029] Legend:

[0030] 1. Cleaning barrel; 11. Barrel body; 12. Chamber; 13. Barrel edge; 14. Drain pipe; 2. Shell cover; 3. First filter mechanism; 31. Shell one; 32. Filter screen one; 33. Drain port; 34. Slot; 4. Second filter mechanism; 41. Shell two; 42. Filter screen two; 5. Third filter mechanism; 51. Shell three; 52. Filter screen three; 53. Snap ring; 6. Driving mechanism; 61. Motor; 62. Magnetic rotor; 63. Copper rotor; 7. Feeding mechanism; 71. Feeding hopper; 72. Valve; 8. Flushing mechanism; 81. Water pump; 82. Connecting hose. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0032] Reference Figure 1-Figure 4The utility model provides an embodiment: a multi-stage filtering device for preparing stem cells, comprising a cleaning barrel 1, the cleaning barrel 1 is composed of a barrel body 11, a chamber 12, a barrel edge 13 and a drain pipe 14, the drain pipe 14 is fixed to the outside of the barrel body 11, the chamber 12 is used to collect the waste liquid generated during the filtration, and then discharge the waste liquid through the drain pipe 14. A shell cover 2 is installed on the top of the barrel body 11, and the shell cover 2 and the barrel body 11 are connected by threads. After the filtration of the stem cells is completed, the shell cover 2 and the barrel body 11 can be separated by the threads. A driving mechanism 6 is installed inside the barrel body 11, and a first filtering mechanism 3, a second filtering mechanism 4 and a third filtering mechanism 5 are placed inside the chamber 12. The first filtering mechanism 3, the second filtering mechanism 4 and the third filtering mechanism 5 are detachably connected, and the first filtering mechanism 3 and the driving mechanism 6 are snap-connected; the driving mechanism 6 can drive the first filtering mechanism 3, the second filtering mechanism 4 and the third filtering mechanism 5 to rotate, so that the stem cell stock solution is centrifuged while being filtered, so that the stock solution is evenly dispersed, thereby avoiding clogging of the filter screen and improving the filtering effect. The first filtering mechanism 3 includes a shell 31, which is located inside the chamber 12, and a filter screen 32 is fixedly connected to the middle of the shell 31. A plurality of drainage ports 33 are provided on the side wall of the shell 31. The stock solution reaching the inside of the shell 31 is separated by the filter screen 32, and then the waste liquid generated by the separation is discharged from the drainage port 33.

[0033] Reference Figure 2-Figure 3 The second filtering mechanism 4 includes a shell 2 41, which is threadedly connected to the top of the shell 1 31. The shell 2 41 and the shell 1 31 can be disassembled, which is convenient for collecting stem cells on the surface of the filter net 1 32, and it is convenient to replace the first filtering mechanism 3 or the second filtering mechanism 4 with different pore sizes as needed. The middle part of the shell 2 41 is fixedly connected with the filter net 2 42, and the original liquid is filtered twice through the filter net 2 42. The third filtering mechanism 5 includes a shell 3 51, which is threadedly connected to the top of the shell 2 41, and the shell 2 41 and the shell 3 51 can be disassembled, which is convenient for collecting stem cells on the surface of the filter net 2 42, and it is convenient to replace the third filtering mechanism 5 or the second filtering mechanism 4 with different pore sizes as needed. The middle part of the shell 3 51 is fixedly connected with the filter net 3 52, and the stem cell original liquid is initially filtered through the filter net 3 52. A snap ring 53 is fixedly connected to the top of the shell three 51, and the snap ring 53 is overlapped on the inner side of the barrel edge 13. After the first filter mechanism 3, the second filter mechanism 4, and the third filter mechanism 5 are connected in sequence and placed in the chamber 12, the snap ring 53 is overlapped on the inner side of the barrel edge 13. In this way, the stability of the first filter mechanism 3, the second filter mechanism 4, and the third filter mechanism 5 during rotation can be guaranteed.

[0034] Reference Figure 4The driving mechanism 6 includes a motor 61, which is fixedly connected to the barrel 11. The output end of the motor 61 is fixedly connected to a magnetic rotor 62. A copper rotor 63 is placed at the bottom of the chamber 12. The copper rotor 63 and the magnetic rotor 62 are connected by magnetic field coupling. When the motor 61 is working, the magnetic rotor 62 is driven to rotate, and the copper rotor 63 can be synchronously rotated at this time. The copper rotor 63 drives the first filter mechanism 3, the second filter mechanism 4, and the third filter mechanism 5 to rotate, so as to perform centrifugal operation. A slot 34 is provided at the bottom of the shell 1 31. The copper rotor 63 is engaged with the slot 34. After the slot 34 and the copper rotor 63 are engaged, the copper rotor 63 can drive the first filter mechanism 3 to rotate. A protrusion is provided at the bottom of the shell 1 31. The copper rotor 63 is located inside the protrusion. The separated waste liquid is guided by the protrusion, so that the waste liquid flows into the chamber 12.

[0035] Reference Figure 1-Figure 2 A feeding mechanism 7 is installed on the top of the shell cover 2, and the feeding mechanism 7 includes a feeding hopper 71 and a valve 72. The feeding hopper 71 is fixedly connected to the top of the shell cover 2, and the bottom of the feeding hopper 71 passes through the shell cover 2 and is connected to the inside of the cleaning barrel 1. The valve 72 is installed in the middle of the feeding hopper 71. The stem cell stock solution to be separated is put into the feeding hopper 71, and the valve 72 is used to adjust the feeding speed of the stock solution so that the stock solution is uniformly transported to the top of the third filtering mechanism 5.

[0036] Reference Figure 1-Figure 2 A flushing mechanism 8 is installed on the outside of the barrel body 11, and the flushing mechanism 8 includes a water pump 81 and a connecting hose 82. The water pump 81 is fixedly connected to the outside of the barrel body 11, and the connecting hose 82 is fixedly connected to the output end of the water pump 81. The other end of the connecting hose 82 passes through the shell cover 2 and is connected to the cleaning barrel 1. The end of the connecting hose 82 away from the water pump 81 is rotatably connected to the middle of the shell cover 2. After the filtration treatment of the raw liquid is completed, the external flushing agent is transported by the water pump 81, and the flushing agent is transported to the inside of the cleaning barrel 1 through the connecting hose 82 to flush the inside of the chamber 12, so as to improve the recovery rate of the target cells, remove residual impurities, and improve the purity of the cells, while reducing the risk of clogging and protecting the cell activity, so as to provide higher efficiency and accuracy for subsequent separation and preparation. The flushing agent is preferably phosphate buffer or cell culture medium, and at the same time, it is ensured to be flushed with a slow flow to avoid mechanical damage to the cells.

[0037] Working principle: When stem cells need to be filtered, the cell stock solution is placed in the hopper 71, and then the valve 72 is opened to cause the stock solution to fall downward into the third filter mechanism 5, and preliminary filtration is performed through the filter mesh 3 52. At this time, the magnetic rotor 62 is driven to rotate by the driving motor 61, and the copper rotor 63 rotates under the influence of the magnetic force, thereby driving the first filter mechanism 3 to rotate as a whole, and the second filter mechanism 4 and the third filter mechanism 5 also rotate together. At this time, the stock solution entering the third filter mechanism 5 passes through the filter mesh 3 52 under the influence of gravity, and is also subjected to centrifugal action, so that the stock solution is dispersed on the surface of the filter mesh 3 52, thereby avoiding The blockage caused by the filter mesh three 52 entering from a single point can improve the filtering efficiency. After multi-stage filtration by the filter mesh three 52, the filter mesh two 42, and the filter mesh one 32, the liquid is discharged from the drain port 33 to the inside of the chamber 12, and finally discharged from the drain pipe 14. At this time, it is only necessary to twist the shell cover 2 to separate the shell cover 2 and the cleaning bucket 1, and the third filtering mechanism 5, the second filtering mechanism 4, and the first filtering mechanism 3 can be taken out from the inside of the cleaning bucket 1 to collect the filtered stem cells. The first filtering mechanism 3, the second filtering mechanism 4 and the third filtering mechanism 5 are detachably connected, which can facilitate the collection of cells on the multi-stage filter mesh and freely combine filter meshes of different pore sizes as needed.

[0038] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A multi-stage filtration device for preparing stem cells, comprising a cleaning bucket (1), characterized in that: The cleaning barrel (1) is composed of a barrel body (11), a chamber (12), a barrel edge (13) and a liquid discharge pipe (14); a shell cover (2) is installed on the top of the barrel body (11); a driving mechanism (6) is installed inside the barrel body (11); a first filtering mechanism (3), a second filtering mechanism (4) and a third filtering mechanism (5) are placed inside the chamber (12); the first filtering mechanism (3), the second filtering mechanism (4) and the third filtering mechanism (5) are detachably connected, and the first filtering mechanism (3) and the driving mechanism (6) are snap-connected; The first filtering mechanism (3) comprises a shell one (31), the shell one (31) being located inside the chamber (12), a filter screen one (32) being fixedly connected to the middle of the shell one (31), and a plurality of liquid discharge ports (33) being provided on the side wall of the shell one (31).

2. A multi-stage filtration device for preparing stem cells according to claim 1, characterized in that: The second filtering mechanism (4) comprises a second shell (41), wherein the second shell (41) is threadedly connected to the top of the first shell (31), and a second filter screen (42) is fixedly connected to the middle of the second shell (41).

3. A multi-stage filtration device for preparing stem cells according to claim 2, characterized in that: The third filtering mechanism (5) comprises a shell three (51), the shell three (51) being threadedly connected to the top of the shell two (41), a filter net three (52) being fixedly connected to the middle of the shell three (51), a snap ring (53) being fixedly connected to the top of the shell three (51), and the snap ring (53) being overlapped on the inner side of the barrel edge (13).

4. The multi-stage filtration device for preparing stem cells according to claim 1, characterized in that: The driving mechanism (6) comprises a motor (61), the motor (61) being fixedly connected inside the barrel body (11), the output end of the motor (61) being fixedly connected to a magnetic rotor (62), a copper rotor (63) being placed at the bottom of the chamber (12), a slot (34) being provided at the bottom of the housing (31), and the copper rotor (63) being snap-fitted to the slot (34).

5. The multi-stage filtration device for preparing stem cells according to claim 1, characterized in that: A feeding mechanism (7) is installed on the top of the shell cover (2), and the feeding mechanism (7) comprises a feeding hopper (71) and a valve (72). The feeding hopper (71) is fixedly connected to the top of the shell cover (2), and the bottom of the feeding hopper (71) passes through the shell cover (2) and is connected to the inside of the cleaning barrel (1). The valve (72) is installed in the middle of the feeding hopper (71).

6. The multi-stage filtration device for preparing stem cells according to claim 1, characterized in that: A flushing mechanism (8) is installed outside the barrel body (11), and the flushing mechanism (8) comprises a water pump (81) and a connecting hose (82). The water pump (81) is fixedly connected to the outside of the barrel body (11), and the connecting hose (82) is fixedly connected to the output end of the water pump (81). The other end of the connecting hose (82) passes through the shell cover (2) and is connected to the cleaning barrel (1).

7. The multi-stage filtration device for preparing stem cells according to claim 4, characterized in that: The inner bottom of the housing (31) is provided with a protrusion, and the copper rotor (63) is located inside the protrusion.

8. The multi-stage filtration device for preparing stem cells according to claim 6, characterized in that: One end of the connecting hose (82) away from the water pump (81) is rotatably connected to the middle of the shell cover (2).

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