Nucleic acid extraction and purification device

Abstract

The utility model relates to the technical field of nucleic acid extraction and purification, and discloses a nucleic acid extraction and purification device which comprises an outer bracket and a tank body, a supporting plate is fixedly mounted on the inner side of the outer bracket, and a temperature control mechanism is arranged on the tank body; the temperature control mechanism comprises an air cylinder, a lifting frame and a heat conduction barrel, the air cylinder drives the lifting plate to ascend and descend, then the tank body is driven to ascend and descend, the position of the tank body relative to the temperature sensor is changed, and then the temperature sensor can measure the temperature of different depths in the tank body. The positions of the heating head and the cooling head in the heat conduction barrel are changed while the tank body ascends and descends, when the temperature of a certain position is measured to be too high or too low, the heat conduction barrel is heated through the heating head or cooled through the cooling head, and then mixed liquid in the tank body is heated and warmed up. The heating or cooling range is expanded through the multiple heat conduction cylinders, and then the temperature control effect is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction and purification, in particular to a nucleic acid extraction and purification device. Background Art

[0002] Nucleic acid extraction and purification are the basis of molecular biology experiments. The method of nucleic acid purification is the most important factor affecting the quality of extracted nucleic acids, and is also the key to the success or failure of downstream molecular biology experiments. At present, the common methods of nucleic acid purification include PC extraction method, high salt precipitation method, centrifugal column method and biological magnetic bead method. These methods have their own advantages and disadvantages. Generally, high-pressure precipitation nucleic acid extraction and purification devices often do not have the function of temperature control, resulting in the inability of proteins to reach the optimal precipitation temperature, which is easy to form residues, affecting the purity of people's extracted nucleic acids and failing to meet people's usage needs. The temperature control method of the scene is relatively simple, and it is impossible to accurately control the temperature according to the different temperatures in different areas. Utility Model Content

[0003] The purpose of the utility model is to provide a nucleic acid extraction and purification device to solve the problems raised in the above background technology.

[0004] To achieve the above object, the utility model provides the following technical solution: a nucleic acid extraction and purification device, comprising an outer bracket and a tank body, a support plate is fixedly installed on the inner side of the outer bracket, and a temperature control mechanism is arranged on the tank body;

[0005] The temperature control mechanism includes a cylinder, a lifting frame, and a heat-conducting tube. The cylinder is fixedly mounted on a support plate. A lifting plate is fixedly mounted on the top of the cylinder. The lifting plate is fixedly mounted on the outer wall of the top end of the tank body. The lifting frame is fixedly mounted on the support plate. A temperature sensor is fixedly mounted on the top of the lifting frame. Four temperature sensors are provided and distributed in a circular array around the axis of the tank body. The heat-conducting tube is fixedly mounted in the tank body. Eight heat-conducting tubes are provided, and every two heat-conducting tubes form a group. A temperature control component is provided inside the heat-conducting tube. The temperature control component includes a heating component and a cooling component. Each heat-conducting tube is provided with a heating component or a cooling component.

[0006] Furthermore, the heating component includes a heating device, a heating head, and a medium circulation pipe. The heating device is fixedly installed on an external bracket. The output end of the heating device is connected to a heat exchange medium flow tank 1 through a pipeline. The heating head and the heat exchange medium flow tank 1 are connected through a medium circulation pipe. The heating head is slidably arranged in the heat conductive cylinder.

[0007] Furthermore, the cooling component includes a refrigeration device, a cooling head, and a medium circulation pipe. The refrigeration device is fixedly installed on an external bracket. The output end of the refrigeration device is connected to a heat exchange medium flow valve 2 through a pipeline. The cooling head and the heat exchange medium flow valve 2 are connected through the medium circulation pipe, and the cooling head is slidably arranged in the heat conductive cylinder.

[0008] Furthermore, a feed pipe is connected to the top of the tank body, a discharge pipe is connected to the bottom of the tank body, a sealing plug is clamped at the end of the discharge pipe, a through groove is opened on the top of the support plate, and the inner diameter of the through groove is larger than the outer diameter of the sealing plug.

[0009] Furthermore, a motor is fixedly installed on the top of the lifting plate, a rotating shaft is fixedly installed on the output end of the motor, the rotating shaft is connected to a stirring shaft through a pulley group, the stirring shaft passes through the top of the tank body through a bearing, and a stirring blade is fixedly installed on the outer wall of the stirring shaft.

[0010] Furthermore, four stirring shafts are provided and distributed in a circular array around the axis of the tank body. A gear is fixedly installed on the outer wall of the stirring shaft, and the gear is meshed with a gear ring. A limiting ring is rotatably sleeved on the outer wall of the gear ring, and the limiting ring is fixedly installed on the top of the tank body.

[0011] Compared with the prior art, the beneficial effects of the utility model are:

[0012] 1. The cylinder drives the lifting plate up and down, and then drives the tank body up and down, changing the position of the tank body relative to the temperature sensor, so that the temperature sensor can measure the temperature at different depths in the tank body. When the tank body is lifted and lowered, the position of the heating head and the cooling head in the heat-conducting tube is also changed. When the temperature at a certain position is measured to be too high or too low, the heating head heats the heat-conducting tube or the cooling head cools the heat-conducting tube, thereby heating and heating the mixed liquid in the tank body. Multiple heat-conducting tubes are used to expand the range of heating or cooling, thereby improving the temperature control effect;

[0013] 2. The motor drives the rotating shaft to rotate, and then drives the stirring shaft to rotate, so that the stirring shaft drives the stirring blades to rotate to stir the mixed liquid. This can not only improve the mixing effect of the mixed liquid, but also make the temperature of all the mixed liquids in the tank consistent through the flow of the mixed liquid, thereby improving the temperature control effect of the mixed liquid in the tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of the utility model;

[0015] Figure 2 This is a structural schematic diagram of the bottom bottom view of the support plate of the utility model;

[0016] Figure 3 It is a structural schematic diagram of the front cross-sectional view of the tank body of the utility model;

[0017] Figure 4 It is a structural schematic diagram of a top sectional view of the tank body of the utility model.

[0018] In the figure: 1. outer bracket; 2. tank body; 3. support plate; 4. temperature control mechanism; 401. cylinder; 402. lifting plate; 403. lifting frame; 404. temperature sensor; 405. heat-conducting tube; 406. temperature control component; 4061. heating equipment; 4062. heat exchange medium flow bin one; 4063. heating head; 4064. refrigeration equipment; 4065. heat exchange medium flow valve two; 4066. cooling head; 4067. medium flow pipe; 5. through groove; 6. feed pipe; 7. motor; 8. rotating shaft; 9. stirring shaft; 10. stirring blade; 11. gear; 12. gear ring; 13. limit ring; 14. discharge pipe; 15. sealing plug. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0020] See also Figure 1-Figure 4 The utility model provides a technical solution: a nucleic acid extraction and purification device, comprising an outer bracket 1 and a tank body 2, a support plate 3 is fixedly installed on the inner side of the outer bracket 1, and a temperature control mechanism 4 is arranged on the tank body 2;

[0021] The temperature control mechanism 4 includes a cylinder 401, a lifting frame 403, and a heat-conducting tube 405. The cylinder 401 is fixedly mounted on the support plate 3. A lifting plate 402 is fixedly mounted on the top of the cylinder 401. The lifting plate 402 is fixedly mounted on the outer wall of the top of the tank body 2. The lifting frame 403 is fixedly mounted on the support plate 3. A temperature sensor 404 is fixedly mounted on the top of the lifting frame 403. Four temperature sensors 404 are arranged and are arranged in a circular array around the axis of the tank body 2. The heat-conducting tube 405 is fixedly mounted in the tank body 2. Eight heat-conducting tubes 405 are arranged, and every two heat-conducting tubes 405 form a group. A temperature control component 406 is arranged inside the heat-conducting tube 405. The temperature control component 406 includes a temperature-raising component and a temperature-reducing component. A heating component is provided in each heat-conducting cylinder 405, and a heating component or a cooling component is provided in each heat-conducting cylinder 405. The heating component includes a heating device 4061, a heating head 4063, and a medium circulation pipe 4067. The heating device 4061 is fixedly installed on the outer bracket 1. The output end of the heating device 4061 is connected to a heat exchange medium flow chamber 4062 through a pipeline. The heating head 4063 is connected to the heat exchange medium flow chamber 4062 through a medium circulation pipe 4067. The heating head 4063 is slidably provided in the heat-conducting cylinder 405. The cooling component includes a cooling device 4064, a cooling head 4066, and a medium circulation pipe 4067. The cooling device 4064 is fixedly installed on the outer bracket 1. The output end of the cooling device 4064 is connected to a heat exchange medium flow valve 4065 through a pipeline. The cooling head 4066 is connected to the heat exchange medium flow valve 4065 through a medium flow pipe 4067. The cooling head 4066 is slidably arranged in the heat-conducting cylinder 405, and the lifting plate 402 is driven up and down by the cylinder 401, thereby driving the tank body 2 up and down, changing the position of the tank body 2 relative to the temperature sensor 404, so that the temperature sensor 404 can measure the temperature at different depths in the tank body 2. When the tank body 2 is lifted or lowered, the position of the heating head 4063 and the cooling head 4066 in the heat-conducting cylinder 405 is also changed. When the temperature at a certain position is measured to be too high or too low, the heating head 4063 and the cooling head 4066 are increased. The heat head 4063 heats the heat-conducting tube 405 or the cooling head 4066 cools the heat-conducting tube 405, thereby heating and increasing the temperature of the mixed liquid in the tank body 2. The range of heating or cooling is expanded by multiple heat-conducting tubes 405, thereby improving the temperature control efficiency. The output end of the heating device 4061 is connected to the heat exchange medium flow chamber 1 4062, and the output end of the refrigeration device 4064 is connected to the heat exchange medium flow valve 2 4065, so that the single heating device 4061 and the heat exchange medium flow chamber 1 4062 can pass the heat exchange medium into the corresponding four heating heads 4063 and cooling heads 4066, thereby heating or cooling the heating heads 4063 and cooling heads 4066;

[0022] A feed pipe 6 is provided at the top of the tank body 2, and a discharge pipe 14 is provided at the bottom of the tank body 2. A sealing plug 15 is clamped at the end of the discharge pipe 14. A through groove 5 is provided at the top of the support plate 3, and the inner diameter of the through groove 5 is larger than the outer diameter of the sealing plug 15. The through groove 5 is provided so that when the tank body 2 moves downward, the discharge pipe 14 and the sealing plug 15 can extend from the through groove 5 instead of contacting the support plate 3.

[0023] A motor 7 is fixedly installed on the top of the lifting plate 402, and a rotating shaft 8 is fixedly installed on the output end of the motor 7. The rotating shaft 8 is connected to a stirring shaft 9 through a pulley group. The stirring shaft 9 passes through the top of the tank body 2 through a bearing. A stirring blade 10 is fixedly installed on the outer wall of the stirring shaft 9. The rotating shaft 8 is driven by the motor 7 to rotate, and then the stirring shaft 9 is driven to rotate, so that the stirring shaft 9 drives the stirring blade 10 to rotate to stir the mixed liquid;

[0024] Four stirring shafts 9 are provided and distributed in a circular array around the axis of the tank body 2. A gear 11 is fixedly installed on the outer wall of the stirring shaft 9, and the gear 11 is meshed with a gear ring 12. A limit ring 13 is rotatably sleeved on the outer wall of the gear ring 12. The limit ring 13 is fixedly installed on the top of the tank body 2. Four stirring shafts 9 are provided for installing four groups of stirring blades 10, so as to stir different positions in the tank body 2 at the same time, increase the stirring range, and thus improve the stirring efficiency. Through one gear ring 12 meshing with four gears 11, only a single feed pipe 6 is needed to drive one stirring shaft 9 to rotate through a motor 7, so that the four stirring shafts 9 can rotate synchronously, and a limit ring 13 is provided to limit the gear ring 12.

[0025] Working principle: When in use, the cylinder 401 is turned on to drive the lifting plate 402 to move up and down, and then drive the tank body 2 to move up and down, changing the position of the tank body 2 relative to the temperature sensor 404, so that the temperature sensor 404 can measure the temperature at different depths in the tank body 2. When the tank body 2 moves up and down, the position of the heating head 4063 and the cooling head 4066 in the heat-conducting tube 405 is also changed. When the temperature at a certain position is measured to be too high or too low, the heating head 4063 heats the heat-conducting tube 405 or the cooling head 4066 cools the heat-conducting tube 405. The temperature is lowered, and the mixed liquid in the tank body 2 is heated and increased. The range of heating or cooling is expanded by multiple heat-conducting tubes 405, thereby improving the temperature control efficiency. When the mixed liquid in the tank body 2 needs to be stirred, the motor 7 is turned on to drive the rotating shaft 8 to rotate, and then the stirring shaft 9 is driven to rotate, so that the stirring shaft 9 drives the stirring blades 10 to rotate to stir the mixed liquid, which can not only improve the mixing effect of the mixed liquid, but also make the temperature of all the mixed liquids in the tank body 2 consistent through the flow of the mixed liquid, thereby improving the temperature control effect of the mixed liquid in the tank body 2.

[0026] Based on the embodiments of the present utility model, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present utility model.

Claims

1. A nucleic acid extraction and purification device, comprising an outer support (1) and a tank (2), characterized in that: A support plate (3) is fixedly mounted on the inner side of the outer bracket (1), and a temperature control mechanism (4) is provided on the tank body (2); The temperature control mechanism (4) comprises a cylinder (401), a lifting frame (403), and a heat-conducting cylinder (405); the cylinder (401) is fixedly mounted on the support plate (3); a lifting plate (402) is fixedly mounted on the top of the cylinder (401); the lifting plate (402) is fixedly mounted on the top outer wall of the tank body (2); the lifting frame (403) is fixedly mounted on the support plate (3); a temperature sensor (404) is fixedly mounted on the top of the lifting frame (403); Four temperature sensors (404) are provided and are distributed in a circular array around the axis of the tank body (2). The heat-conducting tubes (405) are fixedly installed in the tank body (2). Eight heat-conducting tubes (405) are provided, and every two heat-conducting tubes (405) form a group. A temperature control component (406) is provided inside the heat-conducting tube (405). The temperature control component (406) includes a heating component and a cooling component. Each heat-conducting tube (405) is provided with a heating component or a cooling component.

2. A nucleic acid extraction and purification device according to claim 1, characterized in that: The temperature-increasing component comprises a heating device (4061), a heating head (4063), and a medium circulation pipe (4067); the heating device (4061) is fixedly mounted on an outer bracket (1); an output end of the heating device (4061) is connected to a heat exchange medium flow chamber (4062) via a pipeline; the heating head (4063) is connected to the heat exchange medium flow chamber (4062) via a medium circulation pipe (4067); and the heating head (4063) is slidably disposed in a heat-conducting cylinder (405).

3. A nucleic acid extraction and purification device according to claim 1, characterized in that: The cooling component comprises a refrigeration device (4064), a cooling head (4066), and a medium circulation pipe (4067); the refrigeration device (4064) is fixedly mounted on an outer bracket (1); the output end of the refrigeration device (4064) is connected to a heat exchange medium flow valve 2 (4065) via a pipeline; the cooling head (4066) and the heat exchange medium flow valve 2 (4065) are connected via a medium circulation pipe (4067); and the cooling head (4066) is slidably disposed in the heat conductive cylinder (405).

4. A nucleic acid extraction and purification device according to claim 1, characterized in that: The top of the tank body (2) is connected to a feed pipe (6), the bottom of the tank body (2) is connected to a discharge pipe (14), the end of the discharge pipe (14) is clamped with a sealing plug (15), and the top of the support plate (3) is provided with a through groove (5), and the inner diameter of the through groove (5) is larger than the outer diameter of the sealing plug (15).

5. A nucleic acid extraction and purification device according to claim 1, characterized in that: A motor (7) is fixedly mounted on the top of the lifting plate (402); a rotating shaft (8) is fixedly mounted on the output end of the motor (7); the rotating shaft (8) is connected to a stirring shaft (9) via a belt pulley group; the stirring shaft (9) passes through the top of the tank body (2) via a bearing; and a stirring blade (10) is fixedly mounted on the outer wall of the stirring shaft (9).

6. A nucleic acid extraction and purification device according to claim 5, characterized in that: Four stirring shafts (9) are provided and are distributed in a circular array around the axis of the tank body (2); a gear (11) is fixedly mounted on the outer wall of the stirring shaft (9); the gear (11) is meshed with a gear ring (12); a limit ring (13) is rotatably sleeved on the outer wall of the gear ring (12); and the limit ring (13) is fixedly mounted on the top of the tank body (2).

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