Combination structure for adjusting use amount of cap added by enzymic method

Abstract

The utility model belongs to the technical field of adjusting structures, and particularly relates to a combined structure for adjusting the use amount of cap adding in an enzymic method, which comprises an inlet pipe and an outlet pipe, an adjusting combined structure is connected between the inlet pipe and the outlet pipe in parallel, and the adjusting combined structure comprises a pneumatic liquid flow valve, a back pressure valve and a volume coil passage. The pneumatic liquid flow valve is communicated with a back pressure valve through a volume coil passage, the pneumatic liquid flow valve is communicated with an inlet pipe, the back pressure valve is communicated with an outlet pipe, the inlet pipe and the outlet pipe are connected with a flow sensor and a pressure sensor in series, and the flow sensor and the pressure sensor are electrically connected with an industrial control computer. According to the utility model, the requirements on different dosage specifications in different scenes can be flexibly met, various experimental conditions and production requirements are adapted, the application range is expanded, the stable structure and accurate control are favorable for ensuring the stability and consistency of the enzyme-method cap adding process, and errors and fluctuation are reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of regulating structures, in particular to a combined structure for regulating the amount of enzymatic capping. Background Art

[0002] Enzymatic capping is a method for capping RNA after in vitro transcription (IVT). In enzymatic capping, Vaccinia capping enzyme (VCE) is usually used to complete the capping reaction. This enzyme is a heterodimeric RNA capping enzyme composed of a large subunit D1 and a small subunit D12, which can add the Cap0 structure (m7GpppN) to the 5' end of mRNA.

[0003] The enzymatic capping process can be divided into the following steps:

[0004] RNA triphosphatase (TPase) reaction: TPase removes the γ-phosphate from the 5′-triphosphate to generate 5′-diphosphate RNA.

[0005] RNA guanylyltransferase (GTase) reaction: GTase transfers the GMP group from GTP to 5′-diphosphate via a lysine-GMP covalent intermediate.

[0006] Purine-N7 methyltransferase (guanine-N7MTase) reaction: Guanine-N7MTase adds a methyl group to the N7 amine of the guanine cap to form the basic cap structure (Cap0).

[0007] Through the synergistic action of these enzymes, mRNA can be effectively capped, thereby improving its stability and translation efficiency.

[0008] The existing inconvenience in achieving very fine and accurate dosage adjustment leads to unstable or unsatisfactory capping effect; when the dosage needs to be quickly adjusted to adapt to different situations, the combined structure may react slowly, affecting efficiency.

[0009] Therefore, we proposed a combined structure for regulating the amount of enzymatic capping to solve the above problems. Utility Model Content

[0010] 1. Technical issues to be resolved

[0011] In view of the shortcomings of the prior art, the utility model provides a combined structure for adjusting the amount of enzymatic capping, which solves the problems raised in the above-mentioned background technology.

[0012] (II) Technical solution

[0013] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0014] A combined structure for regulating the amount of enzymatic capping comprises an inlet pipe and an outlet pipe, wherein a regulating combined structure is connected in parallel between the inlet pipe and the outlet pipe, wherein the regulating combined structure comprises a pneumatic liquid flow valve, a back pressure valve and a volume coil passage, wherein the pneumatic liquid flow valve is connected to the back pressure valve via the volume coil passage, wherein the pneumatic liquid flow valve is connected to the inlet pipe, wherein the back pressure valve is connected to the outlet pipe, wherein a flow sensor and a pressure sensor are connected in series on the inlet pipe and the outlet pipe, wherein the flow sensor and the pressure sensor are electrically connected to an industrial control computer, wherein the industrial control computer is electrically connected to the pneumatic liquid flow valve and the back pressure valve.

[0015] Furthermore, the volumes of the volume coil passage from left to right are: 100ml, 200ml, 400ml and 800ml.

[0016] Furthermore, the parallel-connected regulating combination structure can form dosage changes of any multiple of 100 ml within the range of 100-1500 ml.

[0017] (III) Beneficial effects

[0018] Compared with the prior art, the utility model provides a combined structure for adjusting the amount of enzymatic capping, which has the following beneficial effects:

[0019] The utility model can flexibly respond to the needs for different dosage specifications in different scenarios, adapt to a variety of experimental conditions and production requirements, and expand its application range. The stable structure and precise control help to ensure the stability and consistency of the enzymatic capping process and reduce errors and fluctuations. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the structure of the utility model;

[0021] Figure 2 This is a system diagram of the utility model.

[0022] In the figure: 1. Inlet pipe; 2. Outlet pipe; 3. Flow sensor; 4. Pressure sensor; 5. Pneumatic liquid flow valve; 6. Volume coil passage; 7. Back pressure valve; 8. Industrial control computer. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Example

[0025] like Figure 1-2 As shown, an enzymatic capping dosage adjustment combination structure proposed in one embodiment of the utility model comprises an inlet pipe 1 and an outlet pipe 2, wherein an adjustment combination structure is connected in parallel between the inlet pipe 1 and the outlet pipe 2, wherein the adjustment combination structure comprises a pneumatic liquid flow valve 5, a back pressure valve 7 and a volume coil passage 6, wherein the pneumatic liquid flow valve 5 is connected to the back pressure valve 7 through the volume coil passage 6, wherein the pneumatic liquid flow valve 5 is connected to the inlet pipe 1, wherein the back pressure valve 7 is connected to the outlet pipe 2, wherein a flow sensor 3 and a pressure sensor 4 are connected in series on the inlet pipe 1 and the outlet pipe 2, wherein the flow sensor 3 and the pressure sensor 4 are electrically connected to an industrial control computer 8, wherein the industrial control computer 8 is electrically connected to the pneumatic liquid flow valve 5 and the back pressure valve 7;

[0026] Inlet pipe 1: As a channel for fluid to enter the system, ensuring the input of raw materials.

[0027] Outlet pipe 2: used to output the regulated fluid to the subsequent links.

[0028] Pneumatic liquid flow valve 5:

[0029] Precise flow control: Liquid flow can be adjusted very finely, increasing or decreasing the flow rate according to actual needs.

[0030] Quick response: Able to respond quickly to control signals and adjust flow status in time.

[0031] Back pressure valve 7:

[0032] Stabilize pressure: Maintain the pressure in the system within a certain range to avoid excessive pressure fluctuations.

[0033] Auxiliary regulation: cooperate with the pneumatic liquid flow valve 5 to achieve precise regulation of the dosage;

[0034] The back pressure valve 7 can prevent the medium from flowing back in the pipeline, thereby protecting the safety of the equipment and system.

[0035] Volume Coil Passage 6:

[0036] Provides a variety of adjustment modes: different volume combinations can meet the changing needs of different specifications and dosages.

[0037] Increased flexibility: enables the system to adapt to a variety of capping dosage situations.

[0038] Flow sensor 3 and pressure sensor 4:

[0039] Real-time monitoring: Continuously monitor the flow and pressure data at inlet pipe 1 and outlet pipe 2.

[0040] Feedback information: The monitoring information is fed back to the industrial control computer 8 in a timely manner to provide an accurate basis for precise control.

[0041] Industrial Control Computer 8:

[0042] Data processing: receiving, analyzing and processing data from sensors.

[0043] Intelligent control: According to the data and preset strategies, precise control instructions are sent to the pneumatic liquid flow valve 5 and the back pressure valve 7 to realize automatic and intelligent dosage adjustment.

[0044] like Figure 1 As shown, in some embodiments, the volumes of the volume coil passage 6 from left to right are: 100 ml, 200 ml, 400 ml and 800 ml;

[0045] Provides a variety of options: 100ml, 200ml, 400ml and 800ml, these different volumes provide a rich combination of possibilities for adjusting the amount of enzymatic capping. According to specific needs, different degrees of flow and dosage control can be achieved by selecting different coil passage parts.

[0046] Meet different specifications: This sequentially changing volume can cover a variety of dosage specifications within a certain range, making the combined structure more adaptable and flexible in dealing with different situations. For example, you can choose to use 100ml alone to adjust a smaller dosage, or use it in combination to achieve precise control of a larger dosage.

[0047] Adapt to complex needs: It provides the hardware foundation for more sophisticated and comprehensive enzymatic capping dosage adjustment, ensuring that the system can operate effectively under different experimental conditions and process requirements.

[0048] like Figure 1 As shown, in some embodiments, the parallel-connected regulating combination structures form dosage changes of any multiple of 100 ml within the range of 100-1500 ml;

[0049] Wide coverage: The dosage can be changed within a large range of 100 to 1500 ml, meeting the needs of enzymatic capping dosage in a variety of different application scenarios.

[0050] Precise multiple specifications: The dosage is changed in multiples of 100ml, making the dosage adjustment more precise and regular, and able to accurately reach the specific dosage setting value.

[0051] Flexible combination: Through different combinations of parallel adjustment combination structures, the dosage can be switched and adjusted quickly and conveniently to adapt to dynamic changes in different experiments or production processes.

[0052] Efficient adaptability: This design can better match the actual needs. Whether it is a smaller dosage of 100ml or a larger dosage close to 1500ml, it can be achieved through reasonable combination, which improves the applicability and practicality of the entire system in different situations.

[0053] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A combined structure for adjusting the amount of enzymatic capping, comprising an inlet pipe (1) and an outlet pipe (2), characterized in that: An adjusting assembly structure is connected in parallel between the inlet pipe (1) and the outlet pipe (2), and the adjusting assembly structure comprises a pneumatic liquid flow valve (5), a back pressure valve (7) and a volume coil passage (6); the pneumatic liquid flow valve (5) is connected to the back pressure valve (7) via the volume coil passage (6); the pneumatic liquid flow valve (5) is connected to the inlet pipe (1); the back pressure valve (7) is connected to the outlet pipe (2); a flow sensor (3) and a pressure sensor (4) are connected in series on the inlet pipe (1) and the outlet pipe (2); the flow sensor (3) and the pressure sensor (4) are electrically connected to an industrial control computer (8); and the industrial control computer (8) is electrically connected to the pneumatic liquid flow valve (5) and the back pressure valve (7).

2. The combined structure for adjusting the amount of enzymatic capping according to claim 1, characterized in that: The volumes of the volume coil passage (6) from left to right are: 100ml, 200ml, 400ml and 800ml.

3. A combined structure for adjusting the amount of enzymatic capping according to claim 2, characterized in that: The parallel-connected regulating combination structure can form dosage changes of any multiple of 100 ml within the range of 100-1500 ml.

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