A continuous large-scale separation and purification equipment modified from a column machine

Abstract

The utility model discloses a continuous large-scale separation and purification equipment modified from a column machine, comprising a column machine, a separation column and an iron frame, wherein the upper and lower ends of the separation column are respectively provided with Luer adapters; an upper polytetrafluoroethylene sieve plate and a lower polytetrafluoroethylene sieve plate are provided in the separation column, and a chromatographic column filler is provided between the upper polytetrafluoroethylene sieve plate and the lower polytetrafluoroethylene sieve plate; the separation column is detachably arranged on the iron frame; the eluent outlet of the column machine is connected to the Luer adapter at the upper end of the separation column through a connecting pipe; the Luer adapter at the lower end of the separation column is connected to the detector of the column machine through a connecting pipe. The continuous large-scale separation and purification equipment modified from the column machine of the utility model increases the single separation volume of the chromatographic column on the basis of the original chromatographic column separation, and can separate and purify products more quickly and efficiently, thereby achieving the purpose of improving efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of chemical technology instruments, in particular to a continuous large-scale separation and purification device modified from a column machine. Background Art

[0002] Flash chromatography is a separation and analysis technique based on the principle of chromatography, and its main features are rapid separation and high efficiency. In flash chromatography, the sample passes through the chromatographic column in a shorter time, so faster analysis speed and higher resolution can be obtained. Flash chromatography is widely used in chemistry, biology, medicine, environmental science and other fields to separate and analyze various substances, such as organic matter, inorganic matter, protein, DNA, etc. It should be noted that the separation effect and resolution of flash chromatography may be affected by factors such as the filler of the chromatographic column, the composition of the mobile phase, the flow rate, etc., so it is necessary to select appropriate conditions according to the specific experimental requirements.

[0003] The column machine is a commonly used laboratory equipment, mainly used for chromatographic column separation in chromatographic separation technology. Its principle is to use the interaction between the stationary phase and the mobile phase to separate the components in the sample in the chromatographic column.

[0004] The composition of the column machine: the injector introduces the sample into the chromatographic column, the chromatographic column is the main place for separation, the detector is used to detect the separated components, and the data system is used to record and analyze the test results.

[0005] The advantages of column machines are mainly reflected in high separation efficiency and high selectivity. They can effectively separate complex samples and selectively separate target substances, which makes column machines widely used in many fields such as compound separation, protein separation, and biomolecule separation.

[0006] However, the chromatographic columns that come with existing laboratory column machines generally separate products below the 100-gram level. Larger mixtures require specially customized special chromatographic columns, which are slightly more expensive for disposable chromatographic columns. Utility Model Content

[0007] The purpose of the utility model is to overcome the defects of the prior art and provide a continuous large-scale separation and purification equipment modified from a column machine. On the basis of the original chromatographic column separation, the single separation volume of the chromatographic column is increased, and the product can be separated and purified more quickly and efficiently, thereby achieving the purpose of improving efficiency.

[0008] The technical solution to achieve the above purpose is: a continuous large-scale separation and purification equipment modified from a column machine, comprising a column machine, a separation column and an iron frame, wherein:

[0009] The upper and lower ends of the separation column are respectively provided with Luer adapters; an upper PTFE sieve plate and a lower PTFE sieve plate are provided in the separation column, and a chromatographic column filler is provided between the upper PTFE sieve plate and the lower PTFE sieve plate;

[0010] The separation column is detachably arranged on the iron frame;

[0011] The eluent outlet of the column machine is connected to the Luer adapter at the upper end of the separation column through a connecting tube;

[0012] The Luer adapter at the lower end of the separation column is connected to the detector of the column machine through a connecting tube.

[0013] In the above-mentioned continuous large-scale separation and purification equipment modified from a column machine, the separation column is supported on the iron frame by two fixed chains arranged one above and one below.

[0014] The above-mentioned continuous large-scale separation and purification equipment modified from a column machine, wherein the iron frame includes an upper flange, a lower flange and a plurality of adjustment support rods, the upper and lower ends of each adjustment support rod are connected to the upper flange and the lower flange one by one, and the bottom end of the lower flange is provided with a support foot; the separation column is clamped between the upper flange and the lower flange.

[0015] In the above-mentioned continuous large-scale separation and purification equipment modified from a column machine, the body of the separation column is made of plastic material.

[0016] The above-mentioned continuous large-scale separation and purification equipment modified from a column machine, wherein the column body of the separation column is made of glass material, a protective column is arranged at the top of the column body of the separation column, and the Luer adapter at the upper end of the separation column is located at the top of the protective column.

[0017] The utility model discloses a continuous large-scale separation and purification equipment converted from a column machine. By converting a column machine used in a laboratory, the column machine is connected to an external separation column through a Luer adapter and a connecting tube, and the separation column is fixed with a fixed iron frame, so that the single sample loading amount is increased, the flow rate of the column machine is controlled, and the single separation amount of the chromatographic column can be increased. The product can be separated and purified more quickly and efficiently, thereby achieving the purpose of improving efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of a continuous large-scale separation and purification device modified from a column machine of the utility model;

[0019] Figure 2 The utility model is a structural schematic diagram of another continuous large-scale separation and purification equipment modified from a column machine. DETAILED DESCRIPTION

[0020] In order to enable those skilled in the art to better understand the technical solution of the utility model, the specific implementation method thereof is described in detail below in conjunction with the accompanying drawings:

[0021] Example 1

[0022] See also Figure 1 Embodiment 1 of the present invention is a continuous large-scale separation and purification device modified from a column machine, comprising a column machine 5, a separation column 3 and an iron frame 2.

[0023] The body of the separation column 3 is made of plastic material, and the upper and lower ends of the separation column 3 are respectively provided with a Luer adapter 11 and a Luer adapter 12; an upper polytetrafluoroethylene sieve plate 61 and a lower polytetrafluoroethylene sieve plate 62 are provided in the separation column 3, and a chromatographic column filler is provided between the upper polytetrafluoroethylene sieve plate 61 and the lower polytetrafluoroethylene sieve plate 62; the separation column 3 is detachably arranged on the iron frame 2, specifically, the iron frame 2 has two fixed chains 4 arranged one above and one below, and the separation column 3 is supported on the iron frame 2 by the two fixed chains 4.

[0024] The eluent outlet of the column machine 5 is connected to the Luer adapter 11 at the upper end of the separation column 3 through the connecting tube 7 ; the Luer adapter 12 at the lower end of the separation column 3 is connected to the detector of the column machine 5 through the connecting tube 7 .

[0025] During operation, the eluent passes through the Luer adapter 11 at the upper end through the connecting tube 7, enters the separation column 3, and after being diverted by the upper PTFE sieve plate 61, enters the chromatographic column filler for elution, passes through the lower PTFE sieve plate 62, and then passes through the Luer adapter 12 and the connecting tube 7, enters the detector of the column machine 5 for detection, and then collects the product. The external separation column 3 has a larger size than the chromatographic column carried by the column machine 5 itself, which can increase the single sample loading amount, control the flow rate of the column machine, and increase the single separation amount of the chromatographic column, which can separate and purify the product more quickly and efficiently, thereby achieving the purpose of improving efficiency.

[0026] Example 2

[0027] See also Figure 2Embodiment 2 of the utility model is a continuous large-scale separation and purification equipment modified from a column machine. The difference from Embodiment 1 is that the iron frame 2 includes an upper flange 21, a lower flange 22 and a plurality of adjustment support rods 23. The upper and lower ends of each adjustment support rod 23 are connected to the upper flange 21 and the lower flange 22 one by one, and a support foot 24 is provided at the bottom end of the lower flange 22. The separation column 3 is clamped between the upper flange 21 and the lower flange 22. The body of the separation column 3 is made of glass. The top of the body of the separation column 3 is provided with a protective column 31. The protective column 31 is located at the top of the upper flange 21. The Luer adapter 11 at the upper end of the separation column is located at the top of the protective column 31. When working, the eluent passes through the Luer adapter 11 at the upper end through the connecting pipe 7, first enters the protective column 31, then enters the separation column 3, and then passes through the upper tetrafluoro sieve plate 61 for diversion, enters the chromatographic column packing for elution, passes through the lower tetrafluoro sieve plate 62, and then passes through the Luer adapter 12 and the connecting pipe 7, enters the detector of the column machine 5 for detection, and collects the product. The external separation column 3 has a larger size than the chromatographic column carried by the column machine 5 itself, which can increase the single sample loading amount, control the flow rate of the column machine, and increase the single separation amount of the chromatographic column, which can separate and purify products more quickly and efficiently, and achieve the purpose of improving efficiency.

[0028] In summary, the utility model is a continuous large-scale separation and purification equipment modified from a column machine. By modifying the column machine used in the laboratory, the chromatographic column of the column machine is connected to the external separation column through a Luer adapter and a connecting tube, and the separation column is fixed with a fixed iron frame. The single sample loading amount is increased, the flow rate of the column machine is controlled, the single separation amount of the chromatographic column can be increased, and the product can be separated and purified more quickly and efficiently, thereby achieving the purpose of improving efficiency.

[0029] Those skilled in the art should recognize that the above embodiments are only used to illustrate the present invention, and are not intended to limit the present invention. As long as they are within the spirit of the present invention, any changes or modifications to the above embodiments will fall within the scope of the claims of the present invention.

Claims

1. A continuous large-scale separation and purification device modified from a column machine, characterized in that: It includes a column machine, a separation column and an iron frame, wherein: The upper and lower ends of the separation column are respectively provided with Luer adapters; an upper PTFE sieve plate and a lower PTFE sieve plate are provided in the separation column, and a chromatographic column filler is provided between the upper PTFE sieve plate and the lower PTFE sieve plate; The separation column is detachably arranged on the iron frame; The eluent outlet of the column machine is connected to the Luer adapter at the upper end of the separation column through a connecting tube; The Luer adapter at the lower end of the separation column is connected to the detector of the column machine through a connecting tube.

2. The continuous large-scale separation and purification equipment modified from a column machine according to claim 1, characterized in that: The separation column is supported on the iron frame by two fixing chains arranged one above and one below.

3. The continuous large-scale separation and purification equipment modified from a column machine according to claim 1, characterized in that: The iron frame comprises an upper flange, a lower flange and a plurality of adjustment support rods, the upper and lower ends of each adjustment support rod are connected to the upper flange and the lower flange one by one, and a support foot is arranged at the bottom end of the lower flange; the separation column is clamped between the upper flange and the lower flange.

4. The continuous large-scale separation and purification equipment modified from a column machine according to claim 1, characterized in that: The column body of the separation column is made of plastic material.

5. The continuous large-scale separation and purification equipment modified from a column machine according to claim 1, characterized in that: The body of the separation column is made of glass material, a protective column is arranged at the top of the body of the separation column, and the Luer adapter at the upper end of the separation column is located at the top of the protective column.

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