A reagent storage and on-site detection device and immunohistochemical instrument

Abstract

The present application relates to a reagent storage and in-place detection device and an immunohistochemical instrument, and relates to the technical field of biological sample processing equipment. The reagent storage and in-place detection device includes: a reagent bottle, a mounting plate and a connecting mechanism. A liquid outlet is provided on the reagent bottle, and an in-place detection member is provided on the mounting plate; the connecting mechanism includes a quick-connect plug and a quick-connect socket. The quick-connect plug is provided on the reagent bottle, and the quick-connect plug is connected to the liquid outlet. The quick-connect socket is provided on the mounting plate, and the quick-connect plug and the quick-connect socket are plugged in and matched; when the quick-connect plug and the quick-connect socket are plugged in, the quick-connect plug and the quick-connect socket are connected, and the quick-connect plug contacts the in-place detection member. By providing an in-place detection member, the present application can send an in-place signal when the reagent bottle is installed in place, so that the immunohistochemical instrument can automatically detect whether the reagent bottle is in place.

Description

Technical Field

[0001] The present application relates to the technical field of biological sample processing equipment, and in particular to a reagent storage and on-site detection device and an immunohistochemical instrument. Background Art

[0002] Immunohistochemistry is an analytical instrument used in the fields of biology, basic medicine, and clinical medicine. It is mainly used for immunohistochemical staining to detect specific antigens or antibodies in cells or tissues. The principle is to mount samples from living tissue on a microscope slide for staining, which can be used to improve the visibility of tissue features. It is an instrument that automates the staining operation.

[0003] Since the number of proteins to be identified is very large and is constantly increasing, and the types of reagents used to stain samples are also increasing, a key feature of immunohistochemistry instruments is how to quickly and effectively extract and transport reagents.

[0004] The existing immunohistochemical analyzer stores the reagents in the corresponding reagent bottles, and when the corresponding reagents are needed, the reagents in the reagent bottles are directly extracted. During the installation process of the reagent bottle, the corresponding liquid outlet on the reagent bottle needs to be plugged into the corresponding interface on the immunohistochemical analyzer, so that the immunohistochemical analyzer can directly and automatically extract the reagents in the reagent bottle.

[0005] However, since reagent bottles often need to be transferred or replaced in order to dump waste liquid from the immunohistochemistry instrument or add new reagents, this operation needs to be repeated many times and is currently mainly manual. It is easy for operating errors to cause the reagent bottles to not be properly plugged in. Once the machine starts working and the reagent bottles are not plugged in, the staining process of the immunohistochemistry instrument will not be able to proceed normally. Therefore, whether the reagent bottles are plugged in directly affects the continuous operation of the immunohistochemistry instrument. Utility Model Content

[0006] The present application provides a reagent storage and in-place detection device and an immunohistochemical instrument, the purpose of which is to detect whether a reagent bottle is plugged in place through an in-place detection component, so that when the reagent bottle is installed, the immunohistochemical instrument can automatically operate after detecting that the reagent bottle is in place through the in-place detection component.

[0007] In the first aspect, the present application provides a reagent storage and in-situ detection device using the following technical solution:

[0008] A reagent storage and in-place detection device comprises: a reagent bottle, a mounting plate and a connecting mechanism, wherein the reagent bottle is provided with a liquid outlet, and the mounting plate is provided with an in-place detection member;

[0009] The connection mechanism includes a quick-connect plug and a quick-connect socket, wherein the quick-connect plug is arranged on the reagent bottle and is connected to the liquid outlet, and the quick-connect socket is arranged on the mounting plate, and the quick-connect plug is plugged and matched with the quick-connect socket;

[0010] When the quick-connect plug is plugged into the quick-connect socket, the quick-connect plug is communicated with the quick-connect socket, and the quick-connect plug abuts against the in-position detection member.

[0011] By adopting the above technical solution, a quick-connect plug is provided on the reagent bottle, and a quick-connect socket is provided on the mounting plate, and the quick-connect plug and the quick-connect socket can quickly connect the reagent bottle to the mounting plate, and since an in-place detection component is provided on the mounting plate, when the reagent bottle is connected to the mounting plate, the quick-connect plug will contact the in-place detection component, thereby triggering the in-place detection component, and the in-place detection component can generate an electrical signal indicating that the reagent bottle is in place, and then send the electrical signal to the immunohistochemistry instrument. Therefore, the in-place detection component can automatically detect whether the reagent bottle is installed in place.

[0012] Optionally, the connection mechanism further comprises an elastic clip, and the elastic clip is arranged on a side of the mounting plate facing the reagent bottle;

[0013] When the quick-connect plug is plugged into the quick-connect socket, the elastic clip clamps the quick-connect plug.

[0014] By adopting the above technical solution, when the quick-connect plug is plugged into the quick-connect socket, the elastic clamp can clamp the plug connector, thereby increasing the stability of the connection between the plug connector and the quick-connect socket.

[0015] Optionally, the quick-connect socket includes a connecting tube, the axial direction of the connecting tube is arranged along the spacing direction of the mounting plate and the reagent bottle, the connecting tube slides along its own axial direction with the mounting plate, and one end of the connecting tube is plugged and matched with the quick-connect plug;

[0016] An elastic reset member is also provided on one side of the mounting plate facing the reagent bottle, one end of the elastic reset member is connected to the mounting plate, and the other end is connected to the connecting pipe.

[0017] By adopting the above technical solution, the quick-connect socket includes a connecting pipe, and the quick-connect plug is plugged into the connecting pipe, so that the quick-connect plug is connected to the quick-connect socket.

[0018] Since the connecting tube slides with the mounting plate along its own axial direction, when the quick-connect plug is clamped with the elastic clamp, the connecting tube can move along its own axial direction so that the quick-connect plug can be clamped into the elastic clamp.

[0019] The reset elastic member is provided so that after the connecting tube slides along its own axial direction, the reset elastic member will reset the connecting tube along its own axial direction, thereby driving the connecting tube to press against the quick-connect plug, thereby increasing the stability of the connection between the quick-connect plug and the quick-connect socket.

[0020] Optionally, a limiting member is sleeved on the outer side of the connecting tube, the limiting member is located on a side of the mounting plate away from the reagent bottle, and the limiting member abuts against the mounting plate along the axial direction of the connecting tube.

[0021] By adopting the above technical solution, the limiting member is arranged on the connecting pipe. When the elastic reset member drives the connecting pipe to reset, the limiting member can resist the mounting plate, thereby limiting the movement distance of the connecting pipe.

[0022] Optionally, the quick-connect plug is detachably connected to the reagent bottle.

[0023] By adopting the above technical solution, the quick-connect plug is detachably connected to the reagent bottle, so it is easy to replace the quick-connect plug.

[0024] Optionally, the reagent bottle is further provided with a liquid injection port, and the reagent bottle is further provided with a bottle cap, the bottle cap is detachably connected to the reagent bottle, and the bottle cap closes the liquid injection port.

[0025] By adopting the above technical solution, the matching arrangement of the liquid injection port and the liquid outlet meets the requirements of injecting and discharging reagents from the reagent bottle. The bottle cap is detachably connected to the reagent bottle, and when necessary, the bottle cap can close the liquid injection port to prevent the reagent in the reagent bottle from leaking out.

[0026] Optionally, the bottle cap is provided with an exhaust hole, and a one-way exhaust valve is inserted into the exhaust hole.

[0027] By adopting the above technical solution, a one-way exhaust valve is installed in the exhaust hole, so that the one-way exhaust valve can exhaust in one direction, ensuring that the internal and external atmospheric pressures of the reagent bottle are consistent, so that when extracting reagents from the reagent bottle or injecting waste liquid into the reagent bottle, the air pressure inside and outside the reagent bottle can be kept consistent, thereby preventing the situation where the exhaust cannot be carried out.

[0028] Optionally, a plurality of reinforcement grooves are provided on two opposite side walls of the reagent bottle, and reinforcement ribs are formed between two adjacent reinforcement grooves.

[0029] By adopting the above technical solution, reinforcing grooves are provided on two opposite sides of the reagent bottle to form reinforcing ribs, thereby increasing the structural strength of the reagent bottle and reducing the deformation of the reagent bottle.

[0030] Optionally, the reagent bottle further includes a second handle, and the second handle is used to push and pull the reagent bottle; and the second handle is arranged on the other side of the reagent bottle relative to the liquid outlet.

[0031] By adopting the above technical solution and providing the second handle, the installation and disassembly of the reagent bottle is facilitated.

[0032] In the second aspect, the present application provides an immunohistochemistry instrument that adopts the following technical solution:

[0033] An immunohistochemical instrument is provided with the above reagent storage and on-site detection device.

[0034] By adopting the above technical solution, the above reagent storage and in-place detection device is used in an immunohistochemistry instrument, and the in-place detection component is used to detect whether the reagent bottle is plugged in place. Therefore, when the reagent bottle is installed, the immunohistochemistry instrument can automatically operate after detecting that the reagent bottle is in place through the in-place detection component, thereby improving work efficiency.

[0035] In summary, the present application includes at least one of the following beneficial technical effects:

[0036] 1. The present application sets up a position detection component. When the reagent bottle is connected to the mounting plate, the quick-connect plug will contact the position detection component and trigger the position detection component. The position detection component can send a position signal when the reagent bottle is installed in place, so that the immunohistochemistry instrument can automatically detect whether the reagent bottle is in place.

[0037] 2. The present application can increase the stability of the connection between the quick-connect plug and the quick-connect socket through the structural setting of the elastic clip and the quick-connect socket.

[0038] 3. The present application can increase the structural strength of the reagent bottle and reduce the deformation of the reagent bottle by means of reinforcing ribs and auxiliary ribs. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is a schematic diagram of the overall structure of the reagent storage and in-situ detection device of the present application;

[0040] Figure 2 yes Figure 1 A partial enlarged schematic diagram of part A.

[0041] Figure 3 It is a partial cross-sectional structural schematic diagram of the liquid outlet position of the reagent bottle of the present application.

[0042] Figure 4 It is a partial structural schematic diagram of the quick-connect socket on the mounting plate of the present application.

[0043] Figure 5 It is a schematic cross-sectional structural diagram of the quick-connect socket of the present application.

[0044] Figure 6 It is a schematic diagram of the exploded structure of the reagent bottle of the present application.

[0045] Figure 7 It is a schematic diagram of the overall structure of the bottle bottom of the reagent bottle of the present application.

[0046] In the figure, 1, reagent bottle; 11, liquid outlet; 12, threaded pipe; 13, reinforcement groove; 14, reinforcement rib; 15, auxiliary groove; 16, auxiliary rib; 17, liquid filling port; 18, bottle cap; 181, exhaust hole; 182, one-way exhaust valve; 19, first handle; 110, second handle; 2, mounting plate; 21, in-place detection member; 3, connecting mechanism; 31, quick-connect plug; 311, connecting head; 312, connecting screw sleeve; 313, clamping ring; 32, quick-connect socket; 321, connecting pipe; 322, floating hole; 323, elastic reset member; 324, limiting member; 3241, limiting ring; 3242, clamp; 3243, fixing ring groove; 325, floating head; 33, elastic clamp; 331, connecting piece; 332, first card; 333, second card. DETAILED DESCRIPTION

[0047] The following is combined with Figure 1 -Attached Figure 7 , further details of this application are given.

[0048] A device for storing and detecting reagents in place, referring to Figure 1 and Figure 2 , including a reagent bottle 1, a mounting plate 2 and a connecting mechanism 3, the connecting mechanism 3 including a quick-connect plug 31 and a quick-connect socket 32, the quick-connect plug 31 is arranged on the reagent bottle 1, the quick-connect plug 31 is connected with the reagent bottle 1, the quick-connect socket 32 ​​is arranged on the mounting plate 2, the quick-connect plug 31 is plugged into the quick-connect socket 32, and when the quick-connect plug 31 is plugged into the quick-connect socket 32, the quick-connect plug 31 is connected with the quick-connect socket 32.

[0049] Therefore, after the mounting plate 2 is installed on the immunohistochemistry instrument, the quick-connect socket 32 ​​is connected to the corresponding pipeline inside the immunohistochemistry instrument. At this time, the reagent bottle 1 is installed so that the quick-connect plug 31 of the reagent bottle 1 is plugged into the quick-connect socket 32. The immunohistochemistry instrument can extract the reagent inside the reagent bottle 1 through the quick-connect plug 31 and the quick-connect socket 32, or inject waste liquid into the reagent bottle 1.

[0050] Reference Figure 1 and Figure 2A position detection member 21 is provided on the side of the mounting plate 2 facing the reagent bottle 1. In this embodiment, the position detection member 21 adopts a micro switch. In addition, the position detection member 21 can also adopt other position sensors such as a proximity switch and a displacement sensor. When the quick-connect plug 31 and the quick-connect socket 32 ​​are plugged in, the quick-connect socket 32 ​​contacts the position detection member 21.

[0051] When the reagent bottle 1 is installed in place, the in-place detection member 21 can generate an electrical signal indicating that the reagent bottle 1 is in place, and then send the electrical signal to the host computer in the immunohistochemical instrument. Therefore, the in-place detection member 21 can automatically detect whether the reagent bottle 1 is installed in place and inform the immunohistochemical instrument.

[0052] Reference Figure 1 and Figure 3 A liquid outlet 11 is provided on the reagent bottle 1, and the liquid outlet 11 is provided on a side of the reagent bottle 1 close to the mounting plate 2, and the liquid outlet 11 is located on the side of the reagent bottle 1 close to the bottle top, so the other side of the reagent bottle 1 relative to the bottle top is the bottle bottom, and the spacing direction between the bottle top and the bottle bottom is the width direction of the reagent bottle 1, and the mounting plate 2 is located on one side of the length direction of the reagent bottle 1.

[0053] Reference Figure 2 and Figure 3 , the reagent bottle 1 is also provided with a threaded tube 12, one end of which is fixedly connected to the reagent bottle 1, and the threaded tube 12 is communicated with the liquid outlet 11. The quick-connect plug 31 includes a connecting screw sleeve 312 and a connector 311, one end of the connecting screw sleeve 312 is sleeved on the outside of the connector 311, and the connecting screw sleeve 312 is connected to the connector 311 by threads, and the other end of the connecting screw sleeve 312 is sleeved on the outside of the threaded tube 12, and the connecting screw sleeve 312 is connected to the threaded tube 12 by threads. At this time, the connector 311, the threaded tube 12 and the liquid outlet 11 are communicated with each other.

[0054] Reference Figure 2 and Figure 4 The quick-connect socket 32 ​​includes a connecting pipe 321 , and the connecting pipe 321 is coaxially arranged with the quick-connect plug 31 .

[0055] Reference Figure 3 and Figure 4 , one end of the connector 311 is plugged into the connecting tube 321 , and the connector 311 is now connected to the connecting tube 321 .

[0056] Reference Figure 3 and Figure 5A floating hole 322 is formed through the side of the mounting plate 2 facing the reagent bottle 1, and the connecting tube 321 is plugged into the floating hole 322, and the connecting tube 321 is slidably connected to the inner wall of the floating hole 322 along its own axial direction. A floating head 325 is provided at the end of the connecting tube 321 away from the connecting head 311, and the connecting tube 321 is sleeved on the outside of the floating head 325, and the connecting tube 321 and the floating head 325 are threadedly connected. At this time, the connecting head 311 is connected to the connecting tube 321 and the floating head 325.

[0057] Therefore, when the mounting plate 2 is installed inside the immunohistochemistry instrument, the floating head 325 is connected to the pipeline system inside the immunohistochemistry instrument, which can ensure that the reagent bottle 1 installed on one side of the mounting plate 2 can achieve the extraction of reagents.

[0058] Reference Figure 2 and Figure 4 The side of the mounting plate 2 facing the reagent bottle 1 is also provided with an elastic clamp 33, and the outer side of the connecting screw sleeve 312 facing the mounting plate 2 is provided with a clamping ring 313, the elastic clamp 33 clamps the connecting screw sleeve 312, and the elastic clamp 33 is tightly pressed against the clamping ring 313 along the axial direction of the connecting screw sleeve 312.

[0059] Specifically, the elastic clip 33 includes a connecting piece 331, which is fixed on the side of the mounting plate 2 facing the reagent bottle 1, one end of the connecting tube 321 passes through the connecting piece 331, and the connecting piece 331 is provided with a first card 332 and a second card 333 at both ends along its own length direction. One end of the first card 332 in the length direction is connected to the connecting piece 331, and the other end is extended toward the reagent bottle 1, and one end of the second card 333 in the length direction is connected to the connecting piece 331, and the other end is extended toward the reagent bottle 1; the first card 332 protrudes toward one side of the second card 333 at the middle position along its own length direction, and the second card 333 protrudes toward one side of the first card 332 at the middle position along its own length direction.

[0060] During the plugging process of the reagent bottle 1, when the quick-connect plug 31 is plugged into the quick-connect socket 32, the quick-connect plug 31 is connected to the quick-connect socket 32, the connecting screw sleeve 312 is located between the first card 332 and the second card 333, the protrusion of the first card 332 and the protrusion of the second card 333 both contact the connecting screw sleeve 312, and the protrusion of the first card 332 and the protrusion of the second card 333 are both located on the side of the snap ring 313 facing the reagent bottle 1 and contact the snap ring 313. At this time, the snap ring 313 just contacts the in-place detection member 21 provided on the mounting plate 2, and the in-place detection member 21 detects that the reagent bottle 1 is in place. Therefore, the elastic clamp 33 helps to limit the quick-connect plug 31 relative to the mounting plate 2, and when the reagent bottle 1 is installed in place, the in-place detection member 21 is triggered.

[0061] In addition, the elastic clip 33 can also adopt a clip structure with a spring or other elastic members.

[0062] Reference Figure 2 and Figure 4 The connecting tube 321 is provided with an elastic reset member 323. In this embodiment, the elastic reset member 323 is a spring. In addition, the elastic reset member 323 can also be a sleeve made of elastic material. The elastic reset member 323 is sleeved on the outside of the connecting tube 321, and one end of the elastic reset member 323 is connected to the mounting plate 2, and the other end is connected to the connecting tube 321.

[0063] Therefore, when installing the reagent bottle 1, when the quick connect plug 31 is plugged into the quick connect plug 31, the connecting tube 321 will slide in the floating hole 322 along its own axial direction away from the reagent bottle 1, ensuring that the quick connect plug 31 can be inserted into the elastic clip 33. When the elastic clip 33 clamps the quick connect plug 31, the elastic reset member 323 resists the connecting tube 321 and slides in the floating hole 322 along its own axial direction toward the reagent bottle 1 until it is reset. Therefore, the elastic reset member 323 can now resist the connecting tube 321 and the quick connect plug 31 to be tightly plugged, thereby increasing the tightness of the connection between the quick connect plug 31 and the quick connect socket 32.

[0064] Reference Figure 5 The outer side of the connecting tube 321 is sleeved with a limiting member 324, and the limiting member 324 is located on the side of the connecting plate away from the reagent bottle 1. The limiting member 324 includes a clamp 3242 and a limiting ring 3241, the limiting ring 3241 is sleeved on the outer side of the connecting tube 321, the clamp 3242 is also sleeved on the outer side of the connecting tube 321, and the clamp 3242 is located on the side of the limiting ring 3241 away from the mounting plate 2 along its own axial direction, and a retaining ring groove 3243 is provided on the outer side wall of the connecting tube 321, the clamp 3242 is sleeved in the retaining ring groove 3243, and the clamp 3242 is in conflict with the retaining ring 3241 along its own axial direction.

[0065] Reference Figure 5 When the connecting tube 321 moves along its own axis in a direction away from the reagent bottle 1, the connecting tube 321 drives the clamp 3242 to move synchronously; and when the connecting tube 321 is reset under the action of the elastic reset member, the clamp 3242 abuts against the limiting ring 3241 along its own axis. Therefore, the setting of the limiting member 324 can limit the reset connecting tube 321.

[0066] In addition, the limit member 324 can also be a limit block fixed to the outer side of the connecting tube 321, or a limit block detachably connected to the outer side of the connecting tube 321. Specifically, any limit member 324 that can contact the mounting plate 2 along the axial direction of the connecting tube 321 is acceptable.

[0067] Reference Figure 6A plurality of reinforcing grooves 13 are provided on both sides of the reagent bottle 1 in the thickness direction. The plurality of reinforcing grooves 13 on the same side of the reagent bottle 1 are arranged in a circle and spaced in sequence. A reinforcing rib 14 is formed between two adjacent reinforcing grooves 13. The length direction of the plurality of reinforcing ribs 14 is arranged along the width direction of the reagent bottle 1, and the length direction of the plurality of reinforcing ribs 14 is arranged along the length direction of the bottle body. The arrangement of the reinforcing rib 14 can increase the structural strength of the reagent bottle 1 and reduce the deformation of the reagent bottle 1.

[0068] Reference Figure 7 The bottom of the reagent bottle 1 is provided with a plurality of auxiliary grooves 15, which are arranged in sequence and spaced apart along the length direction of the reagent bottle 1, and an auxiliary rib 16 is formed between two adjacent auxiliary grooves 15, and the length direction of the auxiliary rib 16 is arranged along the thickness direction of the reagent bottle 1. Therefore, the arrangement of the plurality of auxiliary ribs 16 can cooperate with the reinforcing rib 14 to increase the strength of the reagent bottle 1.

[0069] The reagent bottle 1 is made of transparent material or translucent material, so that the reagent bottle 1 is transparent or translucent. In this way, a lighting lamp is arranged at the installation position of the reagent bottle 1, so that the reagent amount in the reagent bottle 1 can be directly visually tested.

[0070] Reference Figure 6 The top of the reagent bottle 1 is also provided with a liquid injection port 17, which is spaced apart from the liquid outlet 11 along the length direction of the reagent bottle 1. The opening of the liquid injection port 17 allows the reagent to be injected into the reagent bottle 1, and the waste liquid in the reagent bottle 1 can also be poured out through the liquid injection port 17.

[0071] Reference Figure 6 The reagent bottle 1 is also provided with a bottle cap 18, which is connected to the reagent bottle 1 by a thread. When the bottle cap 18 is installed on the reagent bottle 1, the bottle cap 18 closes the liquid injection port 17. The bottle cap 18 is used to open and close the liquid injection port 17.

[0072] Reference Figure 6 The bottle cap 18 is provided with an exhaust hole 181, which penetrates the bottle cap 18, and a one-way exhaust valve 182 is inserted in the exhaust hole 181. The one-way exhaust valve 182 is provided so that the gas in the reagent bottle 1 can be discharged from the reagent bottle 1, while the reagent or waste liquid cannot be discharged from the exhaust hole 181. Therefore, the one-way exhaust valve 182 can exhaust gas in one direction to ensure that the internal and external atmospheric pressures of the reagent bottle 1 are consistent, so that when the reagent in the reagent bottle 1 is extracted or when the waste liquid is discharged into the reagent bottle 1, it will not be impossible to extract due to the atmospheric pressure difference.

[0073] Reference Figure 7The top of the reagent bottle 1 is provided with a first handle 19, and the length direction of the first handle 19 is provided along the length direction of the reagent bottle 1. A second handle 110 is provided on the side of the reagent bottle 1 near the injection port 17 in the length direction, and the length direction of the second handle 110 is provided along the width direction of the reagent bottle 1. The first handle 19 and the second handle 110 are provided to facilitate the installation of the reagent bottle 1 into the corresponding machine, and also facilitate the extraction of the reagent bottle 1 from the corresponding machine.

[0074] The implementation principle of the embodiment of the present application is: in actual use, the mounting plate 2 is installed in the immunohistochemistry instrument. When the reagent bottle 1 is installed in the immunohistochemistry instrument, the quick-connect plug 31 on the reagent bottle 1 is plugged into the quick-connect socket 32 ​​corresponding to the mounting plate 2, and when the reagent bottle 1 is installed in place, the quick-connect plug 31 just contacts the in-place detection part 21. At this time, the in-place detection part 21 is triggered, generates an electrical signal, and sends the electrical signal to the upper computer in the immunohistochemistry instrument, so that the immunohistochemistry instrument can automatically determine that the reagent bottle 1 is in place.

[0075] This embodiment also discloses an immunohistochemical instrument equipped with the above-mentioned reagent storage and on-site detection device.

[0076] The implementation principle of the embodiment of the present application is: when the above-mentioned reagent storage and in-place detection device is used in an immunohistochemical instrument, it can automatically detect whether the reagent bottle 1 is installed in place.

[0077] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A reagent storage and in-situ detection device, characterized in that: include: A reagent bottle (1), a mounting plate (2) and a connecting mechanism (3), wherein the reagent bottle (1) is provided with a liquid outlet (11), and the mounting plate (2) is provided with a position detection member (21); The connecting mechanism (3) comprises a quick-connect plug (31) and a quick-connect socket (32), wherein the quick-connect plug (31) is arranged on the reagent bottle (1) and is in communication with the liquid outlet (11), and the quick-connect socket (32) is arranged on the mounting plate (2), and the quick-connect plug (31) is plugged into and matched with the quick-connect socket (32); When the quick-connect plug (31) is plugged into the quick-connect socket (32), the quick-connect plug (31) is connected to the quick-connect socket (32), and the quick-connect plug (31) abuts against the in-place detection member (21).

2. A reagent storage and in-situ detection device according to claim 1, characterized in that: The connecting mechanism (3) further comprises an elastic clip (33), wherein the elastic clip (33) is arranged on a side of the mounting plate (2) facing the reagent bottle (1); When the quick-connect plug (31) is plugged into and matched with the quick-connect socket (32), the elastic clamp (33) clamps the quick-connect plug (31).

3. A reagent storage and in-situ detection device according to claim 2, characterized in that: The quick-connect socket (32) comprises a connecting tube (321), the axial direction of the connecting tube (321) being arranged along the spacing direction between the mounting plate (2) and the reagent bottle (1), the connecting tube (321) sliding along its own axial direction with the mounting plate (2), and one end of the connecting tube (321) being plug-fitted with the quick-connect plug (31); An elastic reset member (323) is also provided on the side of the mounting plate (2) facing the reagent bottle (1); one end of the elastic reset member (323) is connected to the mounting plate (2), and the other end is connected to the connecting tube (321).

4. A reagent storage and in-situ detection device according to claim 3, characterized in that: A limiting member (324) is sleeved on the outside of the connecting tube (321), the limiting member (324) is located on a side of the mounting plate (2) away from the reagent bottle (1), and the limiting member (324) abuts against the mounting plate (2) along the axial direction of the connecting tube (321).

5. The device for storing and detecting reagents in place according to claim 1, characterized in that: The quick-connect plug (31) is detachably connected to the reagent bottle (1).

6. A reagent storage and in-situ detection device according to claim 1, characterized in that: The reagent bottle (1) is also provided with a liquid injection port (17), and the reagent bottle (1) is also provided with a bottle cap (18), the bottle cap (18) is detachably connected to the reagent bottle (1), and the bottle cap (18) closes the liquid injection port (17).

7. A reagent storage and in-situ detection device according to claim 6, characterized in that: The bottle cap (18) is provided with an exhaust hole (181), and a one-way exhaust valve (182) is inserted into the exhaust hole (181).

8. The device for storing and detecting reagents in place according to claim 1, characterized in that: A plurality of reinforcement grooves (13) are provided on opposite side walls of the reagent bottle (1), and a reinforcement rib (14) is formed between two adjacent reinforcement grooves (13).

9. A reagent storage and in-situ detection device according to any one of claims 1 to 8, characterized in that: The reagent bottle (1) further comprises a second handle (110), wherein the second handle (110) is used to enable the reagent bottle (1) to be pushed or pulled; and the second handle (110) is arranged on the other side of the reagent bottle (1) relative to the liquid outlet (11).

10. An immunohistochemical instrument, characterized in that: A device for storing and detecting reagents in place as described in any one of claims 1 to 9 is installed.

Images