A biological reaction sampling device

Abstract

The utility model relates to the technical field of bioreactors, and specifically to a bioreactor sampling device, comprising a reactor, wherein a bracket is fixedly connected to the bottom end of the reactor, a stirring paddle is rotatably connected to the inner cavity of the reactor, an outer pipe is fixedly connected to the surface of the bottom end of the inner cavity of the reactor, three branch pipes are fixedly connected to the surface of the outer pipe, the inner pipe is fitted inside a limiting plate, the surface of the inner pipe is provided with staggered circular holes, a rotating plate is rotatably connected inside the inner pipe, a pipe cover is clamped on the surface of the inner pipe, a through hole is provided on the surface of the pipe cover, and a hose is fixedly connected to the surface of the through hole; the beneficial effects are: the mutual rotational staggered overlap between the circular hole, the branch pipe and the rotating plate is set, so that sampling of liquids at different heights is realized, and the accuracy of the detection result is improved, and the inner pipe and the outer pipe are both fixed inside the reactor, so that liquid contamination is avoided during the sampling process, and the rotating plate and the pipe cover can be disassembled separately, so that the maintenance efficiency of the sampling device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of bioreactors, in particular to a bioreactor sampling device. Background Art

[0002] A bioreactor is a device system that uses the biological functions of enzymes or organisms to perform biochemical reactions in vitro. It is a biological function simulator, such as a fermentation tank, immobilized enzyme or immobilized cell reactor, etc. It has important applications in wine and pharmaceutical production, concentrated jam, juice fermentation, and organic pollutant degradation.

[0003] In the prior art, in order to monitor and analyze the microorganisms in the bioreactor, it is necessary to use a sampling device to sample the culture fluid in the reactor, connect the sampling tube and the hose, and then fix the hose and the connecting sleeve on the surface of the bioreactor. A sampling tube is provided at the other end of the connecting sleeve. The sampling tube is placed inside the reactor, and the solution flows into the sampling bottle through the sampling tube and the hose to complete the sampling operation.

[0004] However, the reaction states of organisms in solutions at different heights in the bioreactor are different. The sampling tube is placed at a fixed height, and it is impossible to sample the liquid at different depths inside the reactor, which affects the detection structure. Utility Model Content

[0005] The purpose of the utility model is to provide a biological reaction sampling device to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a biological reaction sampling device, comprising a reactor, a bracket fixedly connected to the bottom end of the reactor, a stirring paddle rotatably connected to the inner cavity of the reactor, an outer pipe fixedly connected to the bottom end surface of the inner cavity of the reactor, three branch pipes fixedly connected to the surface of the outer pipe, the inner pipe is fitted inside a limiting plate, three staggered circular holes are provided on the surface of the inner pipe, three rotating plates are rotatably connected inside the inner pipe, a tube cover is clamped on the surface of the inner pipe, a through hole is provided on the surface of the tube cover, a hose is fixedly connected to the surface of the through hole, one end of the hose is plugged into the interior of a sampling bottle, and a clip is provided on the surface of the hose.

[0007] Preferably, a motor is fixedly connected to the surface of the reactor, one end of the motor is fixedly connected to a shaft, the shaft is rotatably connected in the inner cavity of the reactor, and a stirring paddle is fixed to the surface of the shaft.

[0008] Preferably, the outer pipe is a cylindrical structure, and three branch pipe arrays are distributed on the surface of the outer pipe. The outer pipe is connected to the mounting hole opened at the bottom end of the reactor, and a limiting hole is opened on the surface of the mounting hole, and the limiting hole is an annular hole.

[0009] Preferably, the surface of the limiting hole is rotatably connected to a limiting plate, the limiting plate is an annular plate, and the surface of the inner pipe is fixedly connected to the limiting plate.

[0010] Preferably, the inner pipe has a cylindrical structure, a clamping groove is opened on the surface of the inner pipe, the clamping groove is an annular groove, a round rod is fixedly connected to the inner wall surface of the inner pipe, the round rod has a cylindrical structure, and a plurality of clamping strips distributed in a circumferential array are fixedly connected to the surface of the round rod.

[0011] Preferably, the three rotating plates are in an array-distributed square plate structure, the inner wall of the inner pipe fits tightly against the rotating plate, a plug is fixedly connected to the surface of the rotating plate, the plug is in a hollow tubular structure, a plurality of slots are provided on the inner wall surface of the plug, and the surface of the card strip is plugged into the slots.

[0012] Preferably, the tube cover is a plate-like structure with a concave cross section, the inner wall surface of the tube cover is in contact with one end of the rotating plate, and a plurality of elastic clamps are fixedly connected to the surface of the tube cover.

[0013] The plate is an arc-shaped plate structure with an "L" cross section, and one end of the elastic clamp is provided with an inclined surface, and the elastic clamp is clamped on the surface of the clamping groove.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] The biological reaction sampling device proposed by the utility model is provided with a circular hole, a branch pipe and a rotating plate that are rotated and misaligned and overlapped with each other, so that the sampling of liquids at different heights is realized, and the accuracy of the detection result is improved; the inner pipe and the outer pipe are both fixed inside the reactor, so that contamination of the liquid during the sampling process is avoided; the rotating plate and the tube cover can be disassembled separately, so the maintenance efficiency of the sampling device is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is a half-section schematic diagram of the structure of the utility model;

[0018] Figure 3 for Figure 2 A schematic diagram of the structure enlargement in the middle;

[0019] Figure 4 This is a schematic diagram of the internal pipeline structure of the utility model;

[0020] Figure 5 for Figure 4 Enlarged schematic diagram of the structure at point B in the middle.

[0021] In the figure: 1. Reactor; 2. Motor; 3. Bracket; 4. Sampling bottle; 5. Hose; 6. Clamp; 7. Agitator; 8. Branch pipe; 9. External pipe; 10. Limiting hole; 11. Limiting plate; 12. Internal pipe; 13. Round rod; 14. Insert pipe; 15. Turn plate; 16. Pipe cover; 17. Elastic clamp; 18. Clamp groove; 19. Round hole; 20. Card slot; 21. Card strip; 22. Through hole. DETAILED DESCRIPTION

[0022] In order to make the purpose and technical solution of the utility model clearly and completely described, and the advantages more clearly understood, the embodiments of the utility model are further described in detail in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are part of the embodiments of the utility model, not all of the embodiments, and are only used to explain the embodiments of the utility model, and are not used to limit the embodiments of the utility model. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] Embodiment 1

[0024] See also Figure 1 to Figure 2 The utility model provides a technical solution: a biological reaction sampling device, comprising a reactor 1, a bracket 3 is fixedly connected to the bottom end of the reactor 1, a stirring paddle 7 is rotatably connected to the inner cavity of the reactor 1, an outer pipe 9 is fixedly connected to the surface of the inner cavity bottom end of the reactor 1, three branch pipes 8 are fixedly connected to the surface of the outer pipe 9, an inner pipe 12 is fitted inside a limiting plate 11, three staggered circular holes 19 are opened on the surface of the inner pipe 12, three rotating plates 15 are rotatably connected inside the inner pipe 12, a pipe cover 16 is clamped on the surface of the inner pipe 12, and a pipe cap 16 is clamped on the surface of the inner pipe 12. A through hole 22 is provided on the surface of the cover 16, and a hose 5 is fixedly connected to the surface of the through hole 22. One end of the hose 5 is inserted into the interior of the sampling bottle 4, and a clamp 6 is provided on the surface of the hose 5. The circular hole 19, the branch pipe 8 and the rotating plate 15 are arranged to rotate and overlap with each other, so that sampling of liquids at different heights can be achieved and the accuracy of the test results can be improved. The inner pipe 12 and the outer pipe 9 are both fixed inside the reactor 1 to avoid contamination of the liquid during the sampling process. The rotating plate 15 and the tube cover 16 can be disassembled separately, which improves the maintenance efficiency of the sampling device.

[0025] Embodiment 2

[0026] See also Figure 3On the basis of Example 1, in order to achieve the sealing of solutions at different heights, a motor 2 is fixedly connected to the surface of the reactor 1, one end of the motor 2 is fixedly connected to a shaft, the shaft is rotatably connected in the inner cavity of the reactor 1, a stirring paddle 7 is fixed to the surface of the shaft, an outer pipe 9 is a cylindrical structure, three branch pipes 8 are distributed in an array on the surface of the outer pipe 9, the outer pipe 9 is connected with the mounting hole opened at the bottom end of the reactor 1, a limiting hole 10 is opened on the surface of the mounting hole, the limiting hole 10 is an annular hole, the surface of the limiting hole 10 is rotatably connected to a limiting plate 11, the limiting plate 11 is an annular plate, and the surface of the inner pipe 12 is fixedly connected to the limiting plate 11.

[0027] The stirring paddle 7 can stir the solution in the reactor 1. The three branch pipes 8 are distributed on a vertical plane and are spaced at equal distances. The limiting holes 10 and the limiting plates 11 are arranged to rotate in coordination with each other and fit tightly. The rotation of the limiting plates 11 can drive the inner pipe 12 to rotate.

[0028] Embodiment 3

[0029] See also Figures 4 to 5 On the basis of the second embodiment, in order to realize the extraction of solutions at different heights, the inner pipe 12 is in a cylindrical structure, and a clamping groove 18 is provided on the surface of the inner pipe 12, and the clamping groove 18 is an annular groove. A round rod 13 is fixedly connected to the inner wall surface of the inner pipe 12, and the round rod 13 is in a cylindrical structure. A plurality of card strips 21 distributed in a circumferential array are fixedly connected to the surface of the round rod 13. The three rotating plates 15 are in an array-distributed square plate-shaped structure. The inner wall of the inner pipe 12 is tightly fitted with the rotating plate 15, and the surface of the rotating plate 15 is A plug tube 14 is fixedly connected, and the plug tube 14 is a hollow tubular structure. A plurality of slots 20 are provided on the inner wall surface of the plug tube 14, and the surface of the clamping strip 21 is plugged into the slot 20. The tube cover 16 is a plate-like structure with a "concave"-shaped cross section. The inner wall surface of the tube cover 16 is fitted with one end of the rotating plate 15. A plurality of elastic clamps 17 are fixedly connected to the surface of the tube cover 16. The elastic clamps 17 are an arc-shaped plate-like structure with an "L" cross section, and one end of the elastic clamps 17 is provided with an inclined surface. The elastic clamps 17 are clamped on the surface of the clamping slot 18.

[0030] The distance between the circular holes 19 opened on the surface of the inner pipe 12 is equal to the distance between the branch pipes 8, and the angle between each two circular holes 19 is ninety degrees. The three rotating plates 15 are staggered with the circular holes 19, and a circular hole 19 is sandwiched between the two rotating plates 15. The rotating plate 15 and the insert pipe 14 are inserted into the round rod 13. The rotating plate 15 can be disassembled, and the elastic clamping plate 17 is clamped in the clamping groove 18 on the surface of the inner pipe 12, so that the pipe cover 16 can be tightly fitted with one end of the inner pipe 12, and the pipe cover 16 can be rotated to penetrate the through hole 22 and the space surrounded by different rotating plates 15.

[0031] In actual use, first, align the clamping strip 21 with the clamping groove 20, insert the inserting tube 14 into the surface of the round rod 13, make the three rotating plates 15 fit closely with the inner wall of the inner pipe 12, then slide the pipe cover 16 along the wall edge of the inner pipe 12 until the elastic clamping plate 17 is clamped in the clamping groove 18, rotate the inner pipe 12, the inner pipe 12 drives the round rod 13 to rotate, the round rod 13 drives the rotating plate 15 to rotate, the circular hole 19 at the upper end of the inner pipe 12 is aligned with the topmost branch pipe 8, and then rotate the pipe cover 16 so that the through hole 19 on the surface of the pipe cover 16 is aligned with the topmost branch pipe 8. The hole 22 is connected to the closed space surrounded by the circular hole 19 at the upper end and the rotating plate 15. Remove the clamp 6, and the solution at the upper end flows from the branch pipe 8 through the upper circular hole 19 into the sealed space surrounded by the rotating plate 15, and then enters the sampling bottle 4 through the through hole 22 and the hose 5 to complete the sampling of the upper solution. Repeat the above steps to sample the middle and bottom solutions in turn. When the rotating plate 15 needs to be replaced, the circular hole 19 of the inner pipe 12 and the branch pipe 8 are staggered, the pipe cover 16 is opened, and the plugged rotating plate 15 is taken out for cleaning and replacement.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological reaction sampling device, comprising a reactor (1), the bottom end of the reactor (1) being fixedly connected to a bracket (3), characterized in that: The inner cavity of the reactor (1) is rotatably connected to a stirring paddle (7); the bottom surface of the inner cavity of the reactor (1) is fixedly connected to an outer pipe (9); the surface of the outer pipe (9) is fixedly connected to three branch pipes (8); the inner portion of the limiting plate (11) is fitted with an inner pipe (12); the surface of the inner pipe (12) is provided with three staggered circular holes (19); the inner portion of the inner pipe (12) is rotatably connected to three rotating plates (15); the surface of the inner pipe (12) is clamped with a pipe cover (16); the surface of the pipe cover (16) is provided with a through hole (22); the surface of the through hole (22) is fixedly connected to a hose (5); one end of the hose (5) is plugged into the interior of a sampling bottle (4); and a clamp (6) is provided on the surface of the hose (5).

2. A biological reaction sampling device according to claim 1, characterized in that: The surface of the reactor (1) is fixedly connected to a motor (2), one end of the motor (2) is fixedly connected to a shaft, the shaft is rotatably connected in the inner cavity of the reactor (1), and a stirring paddle (7) is fixed to the surface of the shaft.

3. A biological reaction sampling device according to claim 1, characterized in that: The outer pipe (9) is in a cylindrical structure, and the three branch pipes (8) are distributed in an array on the surface of the outer pipe (9). The outer pipe (9) is connected to a mounting hole opened at the bottom end of the reactor (1), and a limiting hole (10) is opened on the surface of the mounting hole. The limiting hole (10) is an annular hole.

4. A biological reaction sampling device according to claim 3, characterized in that: The surface of the limiting hole (10) is rotatably connected to a limiting plate (11), the limiting plate (11) is an annular plate, and the surface of the inner pipe (12) is fixedly connected to the limiting plate (11).

5. A biological reaction sampling device according to claim 1, characterized in that: The inner pipe (12) is in a cylindrical structure, a clamping groove (18) is provided on the surface of the inner pipe (12), the clamping groove (18) is an annular groove, a round rod (13) is fixedly connected to the inner wall surface of the inner pipe (12), the round rod (13) is in a cylindrical structure, and a plurality of clamping strips (21) distributed in a circumferential array are fixedly connected to the surface of the round rod (13).

6. A biological reaction sampling device according to claim 5, characterized in that: The three rotating plates (15) are in a square plate-like structure distributed in an array. The inner wall of the inner pipe (12) is tightly fitted to the rotating plate (15). The surface of the rotating plate (15) is fixedly connected with an insert pipe (14). The insert pipe (14) is in a hollow tube-like structure. The inner wall surface of the insert pipe (14) is provided with a plurality of slots (20). The surface of the clip strip (21) is plugged into the slots (20).

7. A biological reaction sampling device according to claim 5, characterized in that: The tube cover (16) is a plate-like structure with a concave cross section. The inner wall surface of the tube cover (16) is fitted with one end of the rotating plate (15). A plurality of elastic clamps (17) are fixedly connected to the surface of the tube cover (16). The elastic clamps (17) are an arc-shaped plate-like structure with an L-shaped cross section. One end of the elastic clamps (17) is provided with an inclined surface. The elastic clamps (17) are clamped on the surface of the clamping groove (18).

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