A cell culture chamber

Abstract

The utility model provides a cell culture chamber, the cell culture chamber comprises a chamber body and a culture dish tray, the chamber body comprises an upper chamber and a lower chamber which can be opened and closed to each other, the culture dish tray is arranged in the lower chamber; the walls of the upper chamber and the lower chamber are both provided with temperature control units; the wall of the lower chamber is provided with a chamber control system and an air inlet and an air outlet which are connected to a gas mixing module; the temperature control unit control module is used to receive the output signal of the temperature control unit and a first temperature sensor and send the output signal to a power control module; the power control module is used to connect or cut off the power supply of the temperature control unit of the upper chamber according to the output signal of the temperature control unit of the upper chamber, and the output ends of the temperature control unit of the lower chamber and the first temperature sensor are both electrically connected to the power control module. According to the utility model, the problem that the existing cell culture chamber has poor temperature control effect and leads to low cell culture efficiency can be solved.

Description

Technical Field

[0001] The utility model belongs to the field of cell culture, and more specifically relates to a cell culture chamber. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro so that cells can survive, grow, reproduce and maintain their main structure and function. As the main carrier of cell culture, the cell culture chamber can simulate the external environment suitable for cell growth, and is therefore widely used in the field of cell culture.

[0003] However, most of the existing cell culture chambers need to rely on cell culture equipment to uniformly control the temperature of multiple cell culture chambers. This situation results in that when a single cell culture chamber or the main body of the cell culture equipment fails, multiple cell culture chambers will stop operating, reducing the efficiency of cell culture. In addition, the existing cell culture chamber is only equipped with a temperature control module on one side of the chamber, resulting in uneven heating of the chamber, poor temperature control effect, and condensation water, breeding bacteria, and irreversible effects on cell culture. Therefore, the existing cell culture chamber has poor operating stability and cannot meet the needs of cell culture well. Utility Model Content

[0004] The utility model aims to solve the problem that the temperature control effect of the existing cell culture chamber is poor, resulting in low cell culture efficiency.

[0005] In order to achieve the above-mentioned object, the utility model provides a cell culture chamber, which is configured on a cell culture device, wherein the cell culture device comprises a mounting plate for placing the cell culture chamber and a gas mixing module for delivering gas into the cell culture chamber;

[0006] The cell culture chamber comprises a chamber body and a culture dish tray, wherein the chamber body comprises an upper chamber and a lower chamber which can be opened and closed to each other, and the culture dish tray is arranged in the lower chamber;

[0007] Temperature control units are provided in the walls of the upper cabin and the lower cabin;

[0008] A cabin control system and an air inlet and an air outlet connected to the gas mixing module are arranged in the wall of the lower cabin;

[0009] The cabin control system includes a temperature control unit control module, a power control module and a first temperature sensor for detecting the temperature of the lower cabin;

[0010] The temperature control unit control module is used to receive output signals of the temperature control unit and the first temperature sensor and send the output signals to the power control module;

[0011] The power control module is used to connect or cut off the power supply of the temperature control unit of the upper compartment according to the output signal of the temperature control unit of the upper compartment;

[0012] The input end of the temperature control unit control module is electrically connected to the temperature control unit of the lower compartment and the first temperature sensor, and the output end thereof is electrically connected to the power control module.

[0013] Optionally, the temperature control unit includes an electric heating plate and a second temperature sensor.

[0014] Optionally, the electric heating plate is a polyimide electric heating film.

[0015] Optionally, the model of the first temperature sensor is TMP117.

[0016] Optionally, the model of the second temperature sensor is PT1000.

[0017] Optionally, the cell culture chamber includes a contact switch for detecting a closed state of the cell culture chamber, wherein the contact switch includes a conductive block disposed on an end surface of the upper chamber and a conductive rod disposed on an end surface of the lower chamber.

[0018] Optionally, the cabin control system includes a contact switch detection module, and the conductive block and the conductive rod are both electrically connected to the contact switch detection module.

[0019] Optionally, a cabin switch is provided on one side of the cell culture cabin, and the cabin switch includes a return spring provided on one side of the upper cabin, a pressure block provided on the return spring, and a groove provided on one side of the lower cabin and adapted to the structure of the pressure block.

[0020] Optionally, sealing rings are provided on the end surfaces of the upper chamber and the lower chamber.

[0021] Optionally, an indicator light for displaying the operating status of the cell culture chamber is provided on the upper chamber.

[0022] The beneficial effects of the utility model are:

[0023] The cell culture chamber proposed by the utility model is provided with temperature control units in the walls of the upper chamber and the lower chamber, so that the upper chamber and the lower chamber can be heated, the temperature difference between the upper chamber and the lower chamber is reduced, and the generation of condensed water is prevented, so that the temperature in the cell culture chamber is maintained within a range suitable for cell growth, and then through the setting of the temperature control unit control module and the power supply control module, the temperature control unit control module can receive the output signal of the temperature control unit and the first temperature sensor and send the output signal to the power supply control module, so that the power supply control module connects or cuts off the power supply of the temperature control unit according to the output signal. Compared with the existing cell culture chamber, the cell culture chamber of the utility model can realize the power cut-off of the independent temperature control unit, reduce the impact of the failure of a single cell culture chamber on multiple cell culture chambers, thereby improving the efficiency of cell culture, and allowing the cell culture chamber to operate stably to meet the needs of cell culture.

[0024] According to the above content, it can be known that the utility model can effectively solve the problem that the temperature control effect of the existing cell culture chamber is poor, resulting in low cell culture efficiency.

[0025] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The present invention may be better understood by referring to the following description in conjunction with the accompanying drawings, in which the same or similar reference numerals are used throughout the drawings to represent the same or similar components.

[0027] Figure 1 A structural exploded view of a cell culture chamber according to an embodiment of the utility model is shown;

[0028] Figure 2 A schematic structural diagram of a cell culture chamber according to an embodiment of the utility model is shown;

[0029] Figure 3 A cross-sectional view of an upper cabin according to an embodiment of the present invention is shown;

[0030] Figure 4 A cross-sectional view of a lower compartment according to an embodiment of the present invention is shown;

[0031] Figure 5 A schematic structural diagram of a cell culture chamber and a cell culture device according to an embodiment of the utility model is shown;

[0032] Figure 6 A principle block diagram of a cabin control system according to an embodiment of the utility model is shown.

[0033] Reference numerals:

[0034] 1-Cell culture equipment;

[0035] 2-Cell culture chamber;

[0036] 3-Mounting plate;

[0037] 4-Petri dish tray;

[0038] 5-Upper cabin;

[0039] 6-Lower cabin;

[0040] 7-Cabin control system;

[0041] 8- Air inlet;

[0042] 9-Air outlet;

[0043] 10-Electric heating plate;

[0044] 11-conductive block;

[0045] 12-conductive rod;

[0046] 13-return spring;

[0047] 14-briquetting;

[0048] 15-groove;

[0049] 16-Indicator light. DETAILED DESCRIPTION

[0050] In order to enable technicians in the relevant technical field to more fully understand the technical solution of the utility model, the exemplary implementation of the utility model will be described in more comprehensive and detailed in conjunction with the accompanying drawings below. Obviously, the one or more implementations of the utility model described below are only one or more of the specific ways to implement the technical solution of the utility model, and are not exhaustive. It should be understood that other ways belonging to a general utility model concept can be used to implement the technical solution of the utility model, and should not be limited by the exemplary description of the implementation. Based on one or more implementations of the utility model, all other implementations obtained by ordinary technicians in the field without making creative work should fall within the scope of protection of the utility model.

[0051] Example: Figure 1 A structural exploded view of a cell culture chamber according to an embodiment of the utility model is shown; Figure 2 A schematic structural diagram of a cell culture chamber according to an embodiment of the utility model is shown;

[0052] Figure 3 A cross-sectional view of an upper cabin according to an embodiment of the present invention is shown; Figure 4A cross-sectional view of a lower compartment according to an embodiment of the present invention is shown; Figure 5 A schematic structural diagram of a cell culture chamber and a cell culture device according to an embodiment of the utility model is shown; Figure 6 A principle block diagram of a cabin control system according to an embodiment of the utility model is shown.

[0053] Reference Figure 1-6 , an embodiment of the utility model provides a cell culture chamber, which is configured on a cell culture device 1, and the cell culture device 1 includes a mounting plate 3 for placing a cell culture chamber 2 and a gas mixing module for conveying gas into the cell culture chamber 2;

[0054] The cell culture chamber 2 includes a chamber body and a culture dish tray 4. The chamber body includes an upper chamber 5 and a lower chamber 6 which can be opened and closed. The culture dish tray 4 is arranged in the lower chamber 6.

[0055] Temperature control units are provided in the walls of the upper cabin 5 and the lower cabin 6;

[0056] The wall of the lower cabin 6 is provided with a cabin control system 7 and an air inlet 8 and an air outlet 9 connected to the gas mixing module;

[0057] The cabin control system 7 includes a temperature control unit control module, a power control module and a first temperature sensor for detecting the temperature of the lower cabin;

[0058] The temperature control unit control module is used to receive output signals of the temperature control unit and the first temperature sensor and send the output signals to the power control module;

[0059] The power control module is used to connect or cut off the power supply of the temperature control unit of the upper compartment according to the output signal of the temperature control unit of the upper compartment 5;

[0060] The input end of the temperature control unit control module is electrically connected to the temperature control unit of the lower chamber 6 and the first temperature sensor, and the output end thereof is electrically connected to the power control module.

[0061] In one embodiment, the temperature control unit includes an electric heating plate 10 and a second temperature sensor.

[0062] In a specific embodiment, the electric heating sheet 10 is a polyimide electric heating film. It is formed by heat-sealing a polyimide film as an outer insulator and a metal foil or a metal wire as an inner conductive heating element at high temperature and high pressure. The polyimide electric heating film has excellent insulation strength, excellent electrical strength, excellent thermal conductivity efficiency and excellent resistance stability, and can meet the needs of heating the upper and lower cabins.

[0063] In a specific embodiment, the model of the first temperature sensor is TMP117.

[0064] In a specific embodiment, the model of the second temperature sensor is PT1000.

[0065] Specifically, the temperature control unit control module is a PID controller, and the first temperature sensor is a calibration-free temperature sensor. During the use of the cell culture chamber, the preset program of the PID controller can determine whether there is an abnormality in the second temperature sensor of the lower chamber through the output signal of the first temperature sensor, so as to facilitate the repair or replacement of the second temperature sensor of the lower chamber. When the second temperature sensor of the lower chamber fails, the first temperature sensor can still detect the temperature of the lower chamber, and will not trigger the power control module to cut off the power supply to the electric heating plate of the lower chamber, so as to avoid affecting the cell culture. Only when the first temperature sensor and the second temperature sensor of the lower chamber fail, will the power control module be triggered to cut off the power supply to the electric heating plate of the lower chamber. This design also improves the operating stability of the cell culture chamber and is beneficial to cell growth.

[0066] In addition, the power control module requires the cabin control system to output a periodic reversal pulse to work properly. For example, if the calculation period is 10ms, the cabin control system needs to output a 50Hz frequency reversal square wave to supply power to the electric heating plate normally. When the cabin control system fails and stops running, the square wave does not reverse, and the power control module will cut off the power supply to the electric heating plate to prompt the operator to check or repair it. Only when the cabin control system is operating normally, the power control module will continue to supply power to the electric heating plate.

[0067] Of course, it should be appreciated that other models of the electric heating plate, the first temperature sensor and the second temperature sensor may also be used, and the present invention is not limited thereto. Any model that can achieve the same technical effect of the present invention is within the protection scope of the present invention.

[0068] In one embodiment, the cell culture chamber includes a contact switch for detecting the closed state of the cell culture chamber 2 , and the contact switch includes a conductive block 11 arranged on the end surface of the upper chamber 5 , and a conductive rod 12 arranged on the end surface of the lower chamber 6 .

[0069] In one embodiment, the cabin control system 7 includes a contact switch detection module, and the conductive block 11 and the conductive rod 12 are both electrically connected to the contact switch detection module.

[0070] Specifically, when the upper chamber and the lower chamber are pressed together, the conductive block can contact the conductive rod and transmit the contact signal to the contact switch detection module to ensure that the cell culture chamber is in a completely closed state, thereby preventing external impurities from entering the cell culture chamber and affecting cell growth.

[0071] In one embodiment, a cabin switch is provided on one side of the cell culture cabin 2, and the cabin switch includes a reset spring 13 provided on one side of the upper cabin 5, a pressure block 14 provided on the reset spring 13, and a groove 15 provided on one side of the lower cabin 6 and adapted to the structure of the pressure block 14.

[0072] In one embodiment, sealing rings are provided on the end surfaces of the upper chamber 5 and the lower chamber 6 .

[0073] Specifically, the sealing ring is a T-shaped silicone sealing ring, which can be combined with the cabin switch so that the upper cabin and the lower cabin are tightly pressed together to ensure the sealing of the cell culture cabin and reduce the gas consumption of the cell culture cabin.

[0074] In one embodiment, an indicator light 16 for displaying the operating status of the cell culture chamber 2 is disposed on the upper chamber 5 .

[0075] In one embodiment, the cabin control system 7 also includes an indicator light control module.

[0076] Specifically, when an abnormality occurs in the operating status inside the cell culture chamber, the chamber control system can send this abnormal information to the indicator light control module, and the indicator light control module controls the indicator light to turn red to prompt the operator that there is a fault in the cell culture chamber, so that the cell culture chamber can be checked and repaired in time.

[0077] like Figure 5 As shown, the cell culture chamber is installed on the cell culture equipment through a mounting plate, and multiple cell culture chambers can be uniformly powered and independently controlled through the cell culture equipment. When a cell culture chamber fails, only the failed cell culture chamber can be processed without affecting the normal operation of other cell culture chambers, so as to improve the efficiency of cell culture.

[0078] The cell culture chamber proposed by the utility model is provided with temperature control units in the walls of the upper chamber and the lower chamber, so that the upper chamber and the lower chamber can be heated, the temperature difference between the upper chamber and the lower chamber is reduced, and the generation of condensed water is prevented, so that the temperature in the cell culture chamber is maintained within a range suitable for cell growth. Then, through the setting of the temperature control unit control module and the power control module, the temperature control unit control module can receive the output signals of the temperature control unit and the first temperature sensor and send the output signals to the power control module, so that the power control module connects or cuts off the power supply of the temperature control unit according to the output signals. Compared with the existing cell culture chambers, the cell culture chamber of the utility model can realize the power cut-off of the independent temperature control unit, reduce the influence of the failure of a single cell culture chamber on multiple cell culture chambers, and thus improve the efficiency of cell culture.

[0079] Furthermore, by providing a contact switch, a chamber switch and a sealing ring, it is possible to ensure that the cell culture chamber is in a completely closed state, thereby preventing external impurities from entering the cell culture chamber and affecting cell growth, thereby reducing the gas consumption of the cell culture chamber.

[0080] Furthermore, by providing an indicator light and an indicator light control module, when a cell culture chamber fails, the operator can be prompted to check and repair the cell culture chamber in a timely manner.

[0081] Therefore, the cell culture chamber proposed in the present invention can make the operation of the cell culture chamber more stable to meet the needs for cell culture.

[0082] Although one or more embodiments of the utility model are described above, it should be known to those skilled in the art that the utility model can be implemented in any other form without departing from its subject matter and scope. Therefore, the embodiments described above are illustrative rather than restrictive, and many modifications and substitutions are obvious to those skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.

Claims

1. A cell culture chamber, characterized in that: is configured on a cell culture device, the cell culture device comprising a mounting plate for placing the cell culture chamber and a gas mixing module for delivering gas into the cell culture chamber; The cell culture chamber comprises a chamber body and a culture dish tray, wherein the chamber body comprises an upper chamber and a lower chamber which can be opened and closed to each other, and the culture dish tray is arranged in the lower chamber; Temperature control units are provided in the walls of the upper cabin and the lower cabin; A cabin control system and an air inlet and an air outlet connected to the gas mixing module are arranged in the wall of the lower cabin; The cabin control system includes a temperature control unit control module, a power control module and a first temperature sensor for detecting the temperature of the lower cabin; The temperature control unit control module is used to receive output signals of the temperature control unit and the first temperature sensor and send the output signals to the power control module; The power control module is used to connect or cut off the power supply of the temperature control unit of the upper compartment according to the output signal of the temperature control unit of the upper compartment; The input end of the temperature control unit control module is electrically connected to the temperature control unit of the lower compartment and the first temperature sensor, and the output end thereof is electrically connected to the power control module.

2. The cell culture chamber according to claim 1, characterized in that: The temperature control unit includes an electric heating plate and a second temperature sensor.

3. The cell culture chamber according to claim 2, characterized in that: The electric heating sheet is a polyimide electric heating film.

4. The cell culture chamber according to claim 1, characterized in that: The model of the first temperature sensor is TMP117.

5. The cell culture chamber according to claim 2, characterized in that: The model of the second temperature sensor is PT1000.

6. The cell culture chamber according to claim 1, characterized in that: The cell culture chamber comprises a contact switch for detecting a closed state of the cell culture chamber, wherein the contact switch comprises a conductive block arranged on an end surface of the upper chamber and a conductive rod arranged on an end surface of the lower chamber.

7. The cell culture chamber according to claim 6, characterized in that: The cabin control system comprises a contact switch detection module, and the conductive block and the conductive rod are both electrically connected to the contact switch detection module.

8. The cell culture chamber according to claim 1, characterized in that: A cabin switch is arranged on one side of the cell culture cabin, and the cabin switch comprises a return spring arranged on one side of the upper cabin, a pressure block arranged on the return spring, and a groove arranged on one side of the lower cabin and adapted to the structure of the pressure block.

9. The cell culture chamber according to claim 1, characterized in that: Sealing rings are arranged on the end surfaces of the upper chamber and the lower chamber.

10. The cell culture chamber according to claim 1, characterized in that: The upper chamber is provided with an indicator light for displaying the operating status of the cell culture chamber.

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