Pre-cryopreservation device for stem cells

Abstract

The utility model provides a pre-cryopreservation device for stem cells, and relates to the technical field of pre-cryopreservation for stem cells. The cryopreservation cabin is arranged in the cryopreservation barrel; the first adjusting part is connected with the cryopreservation cabin so as to drive the cryopreservation cabin to rotate; the placing part is arranged on the first adjusting part and corresponds to the cryopreservation cabin; the second adjusting part is connected with the placing part so as to change the position angle of the placing part; and the sealing part is arranged on the outer side of the cryopreservation barrel. The first adjusting part is arranged to be matched with the cryopreservation cabin, so that the cryopreservation cabin can rotate, and the stem cell test tube can be located at a position close to the sealing part when being stored or taken, so that the stem cell test tube is convenient to store or take; the first adjusting part is matched with the placing part, so that the placing part descends at a proper constant speed, the temperature of the stem cells in the test tube is slowly and uniformly reduced, and the cell activity is prevented from being influenced by sudden temperature drop.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell pre-freezing, in particular to a stem cell pre-freezing device. Background Art

[0002] Stem cell pre-freezing device is a device used to store stem cells for a long time under low temperature conditions. This device usually includes a heat preservation layer, a transition chamber, a sample chamber, etc. The sample chamber is used to place the cell freezing box, and the freezing chamber is filled with liquid nitrogen carrier for rapid freezing of stem cells to reduce cell metabolic activity and maintain its biological activity.

[0003] The existing cell freezing devices are often not convenient to operate when placing stem cells into or taking them out of the freezing devices, which affects the efficiency of stem cell freezing. Utility Model Content

[0004] In order to solve the above technical problems, the technical solution of the utility model is as follows:

[0005] The embodiment of the utility model provides a pre-freezing device for stem cells, comprising:

[0006] Cryogenic barrel; also includes:

[0007] A freezing chamber, arranged in the freezing barrel;

[0008] A first adjusting part is connected to the freezing chamber to drive the freezing chamber to rotate;

[0009] A placement portion, disposed on the first adjustment portion and corresponding to the freezing chamber;

[0010] A second adjusting portion, connected to the placing portion, to change a position angle of the placing portion;

[0011] The sealing part is arranged on the outside of the freezing barrel.

[0012] Furthermore, the freezing chamber comprises:

[0013] A liquid nitrogen placement box is arranged inside the freezing barrel and fits the inner wall of the freezing barrel;

[0014] Cryopreservation tanks are provided on the liquid nitrogen placing box and are arranged in a plurality of groups, wherein the plurality of groups of cryopreservation tanks are distributed circumferentially;

[0015] An input port is arranged at the top of the liquid nitrogen placement box to be used for filling liquid nitrogen.

[0016] Furthermore, the sealing portion includes:

[0017] A connecting top plate is arranged on the top of the freezing barrel;

[0018] A connecting bottom plate is arranged at the bottom end of the freezing barrel;

[0019] A sealing door rotatably connected to the freezing barrel via a first connecting ear;

[0020] A handle is arranged on the sealing door.

[0021] Furthermore, the first adjusting unit includes:

[0022] A first motor is disposed on the connecting bottom plate, and an output end of the first motor is connected to the bottom end of the liquid nitrogen placement box;

[0023] A spline rod, one end of which is connected to the top of the liquid nitrogen placement box;

[0024] A spline sleeve, sleeved on the spline rod;

[0025] A rotating disk, one end of which is connected to the other end of the spline rod, and the top end of the rotating disk is rotatably connected to the connecting top plate;

[0026] The first electric telescopic rod is arranged on the rotating disk.

[0027] Further, the placement unit includes:

[0028] A first connecting rod, one end of which is connected to the spline sleeve, and a top end of which is connected to the output end of the first telescopic rod;

[0029] A second connecting ear, rotatably connected to the first connecting rod;

[0030] A storage member connected to the second connecting ear for placing a test tube;

[0031] There are several groups of the placement parts, and several groups of the placement parts correspond to the freezing tanks.

[0032] Furthermore, the storage device includes:

[0033] a storage rack connected to the second connecting ear;

[0034] Test tube placement slots are arranged on the storage rack and are provided in a plurality of groups. The plurality of groups of test tube placement slots are distributed circumferentially.

[0035] Furthermore, the second adjusting unit includes:

[0036] A third connecting ear is provided on the spline sleeve;

[0037] A fourth connecting ear is arranged on the storage rack;

[0038] The second electric telescopic rod has one end rotatably connected to the third connecting ear; and the other end rotatably connected to the fourth connecting ear.

[0039] The above solution of the utility model includes at least the following beneficial effects:

[0040] The utility model provides a first adjusting part to cooperate with the freezing chamber so that the freezing chamber can rotate. When storing or taking out, the stem cell test tube can be placed close to the sealing part, thereby facilitating storage or taking out. The first adjusting part is provided to cooperate with the placement part so that the placement part can be uniformly lowered at a suitable speed, so that the temperature of the stem cells in the test tube can be slowly and evenly lowered, thereby avoiding a sudden drop in temperature that affects the cell activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a first stereoscopic diagram of the stem cell pre-freezing device of the utility model;

[0042] Figure 2 This is a second stereoscopic view of the stem cell pre-freezing device of the utility model;

[0043] Figure 3 This is a first partial stereoscopic diagram of the stem cell pre-freezing device of the utility model;

[0044] Figure 4 This is a second partial stereoscopic view of the stem cell pre-freezing device of the utility model;

[0045] Figure 5 It is a three-dimensional diagram of the first adjustment part of the pre-freezing device for stem cells of the utility model;

[0046] Figure 6 It is a three-dimensional diagram of the second adjustment part and the placement part of the pre-freezing device for stem cells of the present invention.

[0047] Description of reference numerals:

[0048] 1. Cryogenic barrel; 102. Connecting top plate; 103. Connecting bottom plate; 104. Sealing door; 105. First connecting ear; 106. Handle; 201. Liquid nitrogen placement box; 202. Cryogenic tank; 203. Input port; 301. First motor; 302. Spline rod; 303. Rotating disk; 304. Spline sleeve; 4. First electric telescopic rod; 501. First connecting rod; 502. Second connecting ear; 503. Third connecting ear; 504. Second electric telescopic rod; 505. Fourth connecting ear; 506. Storage rack; 507. Test tube placement tank. DETAILED DESCRIPTION

[0049] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0050] like Figures 1 to 6 As shown, an embodiment of the utility model provides a pre-freezing device for stem cells, comprising: a freezing barrel 1; further comprising: a freezing chamber, which is arranged in the freezing barrel 1; a first adjusting part, which is connected to the freezing chamber to drive the freezing chamber to rotate; a placing part, which is arranged on the first adjusting part and corresponds to the freezing chamber; a second adjusting part, which is connected to the placing part to change the position angle of the placing part; and a sealing part, which is arranged on the outside of the freezing barrel 1.

[0051] In the embodiment of the utility model, the first adjusting part is provided to cooperate with the freezing chamber so that the freezing chamber can be rotated. When storing or taking out, the stem cell test tube can be placed close to the sealing part, thereby facilitating storage or taking out. The first adjusting part is provided to cooperate with the placing part so that the placing part can be slowly placed in the freezing chamber, thereby avoiding the temperature from dropping too fast and affecting the activity of the stem cells.

[0052] like Figures 1 to 6 As shown, the freezing chamber includes: a liquid nitrogen placement box 201, which is arranged inside the freezing barrel 1 and fits against the inner wall of the freezing barrel 1; freezing tanks 202, which are opened on the liquid nitrogen placement box 201 and are provided in a plurality of groups, and the plurality of groups of freezing tanks 202 are distributed circumferentially; an input port 203, which is arranged at the top of the liquid nitrogen placement box 201 and is used to fill liquid nitrogen.

[0053] In the embodiment of the utility model, the liquid nitrogen placement box 201 is set to be hollow, and liquid nitrogen is placed inside, so as to ensure that the temperature in the freezing tank 202 is low enough; by providing an input port 203, when it is detected that the liquid nitrogen is insufficient, the liquid nitrogen is transported through the input port 203.

[0054] like Figures 1 to 6 As shown, the sealing portion includes: a connecting top plate 102, which is arranged at the top end of the freezing barrel 1; a connecting bottom plate 103, which is arranged at the bottom end of the freezing barrel 1; a sealing door 104, which is rotatably connected to the freezing barrel 1 via a first connecting ear 105; and a handle 106, which is arranged on the sealing door 104.

[0055] In the embodiment of the utility model, the sealing door 104 is provided. After the stem cell test tube is placed in the device, the sealing door 104 is closed to seal the device as a whole, thereby ensuring the freezing temperature.

[0056] like Figures 1 to 6 As shown, the first adjusting part includes: a first motor 301, which is arranged on the connecting bottom plate 103, and the output end is connected to the bottom end of the liquid nitrogen placement box 201; a spline rod 302, one end of which is connected to the top end of the liquid nitrogen placement box 201; a spline sleeve 304, which is sleeved on the spline rod 302; a rotating disk 303, one end of which is connected to the other end of the spline rod 302, and the top end of the rotating disk 303 is rotatably connected to the connecting top plate 102; a first electric telescopic rod 4, which is arranged on the rotating disk 303.

[0057] In the embodiment of the utility model, the first motor 301 is started to drive the liquid nitrogen placement box 201 to rotate. The rotation of the liquid nitrogen placement box 201 will drive the liquid nitrogen inside the liquid nitrogen placement box 201 to rotate, thereby improving the heat conversion of the liquid nitrogen, so that the temperature is sufficiently low during freezing; the liquid nitrogen placement box 201 drives the spline rod 302 to rotate, thereby driving the spline sleeve 304 to rotate, thereby driving the placement part and the liquid nitrogen placement box 201 to rotate synchronously.

[0058] like Figures 1 to 6 As shown, the placement portion includes: a first connecting rod 501, one end of which is connected to the spline sleeve 304, and the top end is connected to the output end of the first telescopic rod; a second connecting ear 502, rotatably connected to the first connecting rod 501; a storage piece, connected to the second connecting ear 502, for placing a test tube; wherein the placement portion is provided with a plurality of groups, and a plurality of groups of the placement portions correspond to the freezing tanks 202.

[0059] In the embodiment of the utility model, the storage piece is connected to the second connecting ear 502, and can be rotated around the contact point between the first connecting rod 501 and the second connecting ear 502, and cooperate with the second adjustment part to adjust the angle of the storage piece, so that when storing, compared with the traditional horizontal placement, the test tube placement efficiency can be improved and placement errors can be avoided.

[0060] Among them, after the test tube is placed in the storage piece, the first electric telescopic rod 4 is started to drive the first connecting rod 501 to the lower end, so that the spline sleeve 304 moves downward around the spline rod 302, thereby driving the storage piece to move into the freezing tank 202. By controlling the moving speed of the first electric telescopic rod 4, the storage piece is moved to the freezing tank 202 at a certain speed to avoid the temperature dropping too fast and affecting the activity of stem cells.

[0061] like Figures 1 to 6 As shown, the storage component includes: a storage rack 506 connected to the second connecting ear 502; and test tube placement slots 507, which are opened on the storage rack 506 and are provided in a plurality of groups. The plurality of groups of test tube placement slots 507 are distributed circumferentially.

[0062] In the embodiment of the utility model, the test tube is placed in the test tube placement groove 507, and the test tube placement groove 507 is provided with a heat-insulating material inside to keep the test tube warm.

[0063] like Figures 1 to 6 As shown, the second adjustment portion includes: a third connecting ear 503, which is arranged on the spline sleeve 304; a fourth connecting ear 505, which is arranged on the storage rack 506; a second electric telescopic rod 504, one end of which is rotatably connected to the third connecting ear 503; and the other end of which is rotatably connected to the fourth connecting ear 505.

[0064] In the embodiment of the utility model, when it is necessary to store test tubes, several groups of second electric telescopic rods 504 are started at the same time to shrink the second electric telescopic rods 504, driving the storage rack 506 to rotate upward along the contact point between the first connecting rod 501 and the second connecting ear 502; thereby driving the storage rack 506 to tilt outward as a whole, making it convenient for the storage rack 506 to manually place the test tubes inward. After the test tubes are placed, the second electric telescopic rods 504 are extended and the storage rack 506 is reset; then the first electric telescopic rod 4 is started to drive the storage rack 506 to move downward into the freezing tank 202, thereby completing the freezing.

[0065] Working principle: by simultaneously starting a plurality of groups of second electric telescopic rods 504, the second electric telescopic rods 504 are contracted, driving the storage rack 506 to rotate upward along the contact point between the first connecting rod 501 and the second connecting ear 502; thereby driving the storage rack 506 to tilt outward as a whole, making it convenient for the storage rack 506 to manually place the test tube inward; after a storage rack 506 is placed, the first motor 301 is started to drive the liquid nitrogen placement box 201 to rotate, and the liquid nitrogen placement box 201 drives the spline rod 302 to rotate, thereby driving the spline sleeve 304 to rotate, thereby driving the placement part and the liquid nitrogen placement box 201 to rotate synchronously, so that the lower A storage rack 506 rotates in front of the staff; after all the test tubes are placed, the sealing door 104 is closed, and the second electric telescopic rod 504 is started again to extend the second electric telescopic rod 504, thereby resetting the storage rack 506; finally, the first electric telescopic rod 4 is started to drive the first connecting rod 501 to the lower end, so that the spline sleeve 304 moves downward around the spline rod 302, thereby driving the storage piece to move into the freezing tank 202, and by controlling the moving speed of the first electric telescopic rod 4, the storage piece is moved to the freezing tank 202 at a certain speed to avoid the temperature dropping too fast and affecting the activity of stem cells.

[0066] The above is a preferred embodiment of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A pre-freezing device for stem cells, comprising: A freezing barrel (1); characterized in that it also comprises: A freezing chamber, arranged in the freezing barrel (1); A first adjusting part is connected to the freezing chamber to drive the freezing chamber to rotate; A placement portion, disposed on the first adjustment portion and corresponding to the freezing chamber; A second adjusting portion, connected to the placing portion, to change a position angle of the placing portion; A sealing portion is arranged on the outside of the freezing barrel (1).

2. The stem cell pre-freezing device according to claim 1, characterized in that: The freezing chamber comprises: A liquid nitrogen placement box (201) is arranged inside the freezing barrel (1) and is in contact with the inner wall of the freezing barrel (1); The freezing tanks (202) are opened on the liquid nitrogen placement box (201) and are provided in a plurality of groups, wherein the plurality of groups of the freezing tanks (202) are distributed around the circumference; An input port (203) is arranged at the top of the liquid nitrogen placement box (201) for filling liquid nitrogen.

3. The stem cell pre-freezing device according to claim 2, characterized in that: The sealing portion comprises: A connecting top plate (102) is arranged on the top of the freezing barrel (1); A connecting bottom plate (103) is arranged at the bottom end of the freezing barrel (1); A sealing door (104) is rotatably connected to the freezing barrel (1) via a first connecting ear (105); A handle (106) is arranged on the sealing door (104).

4. The stem cell pre-cryopreservation device according to claim 3, characterized in that: The first adjustment unit includes: A first motor (301) is disposed on the connecting bottom plate (103), and its output end is connected to the bottom end of the liquid nitrogen placement box (201); A spline rod (302), one end of which is connected to the top of the liquid nitrogen placement box (201); A spline sleeve (304) sleeved on the spline rod (302); A rotating disk (303), one end of which is connected to the other end of the spline rod (302), and the top end of the rotating disk (303) is rotatably connected to the connecting top plate (102); The first electric telescopic rod (4) is arranged on the rotating disk (303).

5. The stem cell pre-cryopreservation device according to claim 4, characterized in that: The placement unit includes: A first connecting rod (501), one end of which is connected to the spline sleeve (304), and a top end of which is connected to the output end of the first telescopic rod; A second connecting ear (502) is rotatably connected to the first connecting rod (501); A storage piece connected to the second connecting ear (502) for placing a test tube; There are several groups of placement parts, and several groups of placement parts correspond to the freezing tanks (202).

6. The stem cell pre-cryopreservation device according to claim 5, characterized in that: The storage unit comprises: A storage rack (506) connected to the second connecting ear (502); The test tube placement slots (507) are provided on the storage rack (506) and are arranged in a plurality of groups. The plurality of groups of the test tube placement slots (507) are distributed around the circumference.

7. The stem cell pre-cryopreservation device according to claim 6, characterized in that: The second adjusting unit includes: A third connecting ear (503) is arranged on the spline sleeve (304); A fourth connecting ear (505) is arranged on the storage rack (506); The second electric telescopic rod (504) has one end rotatably connected to the third connecting ear (503) and the other end rotatably connected to the fourth connecting ear (505).

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