Cell centrifugal collector

Abstract

The utility model discloses a cell centrifugal collector, and relates to the technical field of animal cell experiments, in particular to a cell centrifugal collector which comprises a bottom tube, a connecting tube, an intercepting assembly, a limiting assembly, a sleeve, a collecting rubber leather bag, a movable hole, a control rod and a tube cap, the connecting tube is arranged on the upper side of the outer portion of the bottom tube, and the tube cap is arranged on the upper side of the outer portion of the connecting tube. A collecting rubber leather pocket is arranged on the lower side of the interior of the bottom pipe, a movable hole is formed in the lower side of the exterior of the bottom pipe, an intercepting assembly is arranged in the bottom pipe, a sleeve is arranged on the lower side of the exterior of the bottom pipe, a control rod is arranged on the lower side of the exterior of the collecting rubber leather pocket, and a limiting assembly is arranged in the bottom pipe. Compared with the prior art, the device disclosed by the utility model has the advantages that upper-layer clarified liquor after centrifugation can be conveniently poured out; and cell clusters at the bottom can be conveniently taken out.

Description

Technical Field

[0001] The utility model relates to the technical field of animal cell experiments, in particular to a cell centrifugal collector. Background Art

[0002] In biological science research and clinical medicine, centrifugal collection of cells is a common experimental operation. Animal cells and culture medium are packed into multiple centrifuge tubes. The cell culture medium is layered after centrifugation, and the upper clear liquid is poured out. Then, the cell pellets in the multiple tubes are collected and resuspended.

[0003] A novel centrifugal collector for bone marrow cells described in announcement number CN220485701U comprises a sealing plug cover, an opening, a connecting piece, a collecting cylinder, a bottom cone and a fusion joint. The novel centrifugal collector for bone marrow cells is provided by optimizing the setting of the collecting cylinder, wherein the high arc-shaped opening and the offset arc-shaped opening are spirally arranged, and a plurality of arc-shaped structures cooperate to form a vortex-shaped structure, which is convenient for efficient centrifugal treatment and has a good treatment effect; the sealing plug cover is optimized, the outer end surface of the cylinder is provided with frosted patterns, and the center is provided with a through opening, so that after the cylinder is plugged into the opening, it is elastically contracted and fastened to the top end of the collecting cylinder, and the sealing connectivity of the top plug cover is good.

[0004] Although the above-mentioned cell centrifugal collector solves certain problems, it still has the following disadvantages:

[0005] (1) After the centrifugal treatment of the above-mentioned cell centrifugal collector is completed, the upper layer of the clarified liquid needs to be poured out slowly to avoid pouring out the animal cell agglomerates. The operation needs to be more cautious and the operation is inconvenient.

[0006] (2) When the above-mentioned cell centrifugal collector collects the cell clusters precipitated in multiple tubes, the cell clusters tend to adhere to the bottom of the tubes and cannot be poured out. In addition, since the tubes are long, it is generally inconvenient to scrape them. Utility Model Content

[0007] The utility model aims to provide a cell centrifugal collector.

[0008] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0009] A cell centrifugal collector, comprising:

[0010] A bottom tube, wherein the lower end of the bottom tube is tapered, a collecting rubber leather pocket is provided on the lower inner side of the bottom tube, the upper outer edge of the collecting rubber leather pocket is fixedly connected to the inner wall of the bottom tube, a movable hole is provided on the lower outer side of the bottom tube, a control rod is provided inside the movable hole, and the upper end of the control rod is fixedly connected to the lower outer side of the collecting rubber leather pocket;

[0011] A connecting pipe, the connecting pipe is arranged on the upper side of the bottom pipe, the lower end of the connecting pipe is threadedly connected with the upper end of the bottom pipe, a pipe cap is arranged on the upper side of the connecting pipe, and the lower side of the pipe cap is threadedly connected with the upper end of the connecting pipe;

[0012] An interception assembly, the interception assembly is arranged between the bottom tube and the connecting tube, the interception assembly comprises a clamp ring, a movable interception block, a support column and a contact ball, the clamp ring is arranged at the lower side of the connecting tube, the outer edge of the clamp ring is fixedly connected to the inner wall of the connecting tube, the movable interception block is arranged inside the bottom tube, the support column is fixedly connected to the lower side of the movable interception block, the contact ball is fixedly connected to the lower end of the support column, and the contact ball is adapted to the inside of the collecting rubber leather bag;

[0013] A limiting assembly, the limiting assembly being arranged between the supporting column and the bottom tube;

[0014] The sleeve is arranged on the lower side of the outer part of the bottom tube, the sleeve is sleeved on the lower side of the outer part of the bottom tube, and the control rod is matched with the inner part of the sleeve.

[0015] Furthermore, the outer upper surface of the clamp ring is inclined inward, the movable intercepting block is umbrella-shaped, and a sealing rubber ring used in conjunction with the bottom tube is fixedly connected to the outer lower side of the clamp ring.

[0016] Furthermore, the outer diameter of the movable intercepting block is smaller than the inner diameter of the bottom pipe, and the outer diameter of the movable intercepting block is larger than the inner diameter of the clamping ring.

[0017] Furthermore, a pressing block is fixedly connected to the lower end of the control rod at the lower side of the outer portion of the bottom tube, and the control rod is slidably connected to the inside of the movable hole.

[0018] Furthermore, the limiting component comprises:

[0019] Connecting rods, a plurality of connecting rods are evenly distributed and fixedly connected to the outside of the supporting column;

[0020] A plurality of support blocks are evenly distributed inside the bottom tube, the support block is fixedly connected to the other end of the connecting rod connected to the support column, and the side of the support block away from the connecting rod is slidably connected to the inner wall of the bottom tube.

[0021] The utility model provides a cell centrifugal collector, which has the following beneficial effects:

[0022] (1) The bottom tube is blocked by combining the clamp ring and the movable interception block, and the clarified liquid in the connecting tube is poured out. The clarified liquid can be poured out quickly, and the settled cell agglomerates are retained inside the bottom tube, which is easy to operate.

[0023] (2) The cell clusters are gathered together by the collecting rubber bag on the lower side of the bottom tube. After pouring out the clarified liquid, the bottom tube is poured out. By quickly pressing the pressing block or bouncing the pressing block, the cell clusters deposited inside the bottom tube can be easily poured out. The collector is segmented. After the bottom tube and the connecting tube are disassembled, the bottom tube is shorter and easier to scrape. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the implementation or the prior art description. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0025] Figure 1 The utility model is a three-dimensional cell centrifugal collector Figure 1 .

[0026] Figure 2 The utility model is a three-dimensional cell centrifugal collector Figure 2 .

[0027] Figure 3 The utility model is a schematic diagram of the explosion structure of a cell centrifugal collector.

[0028] Figure 4 It is a partial structure stereoscopic of a cell centrifugal collector of the utility model. Figure 1 .

[0029] Figure 5 It is a partial structure stereoscopic of a cell centrifugal collector of the utility model. Figure 2 .

[0030] Figure 6 It is a partial structure stereoscopic of a cell centrifugal collector of the utility model. Figure 3 .

[0031] Figure 7 It is a partial structural enlarged stereoscopic diagram of a cell centrifugal collector of the utility model.

[0032] Markings in the figure: 1. bottom tube; 2. connecting tube; 3. interception assembly; 301. clamping ring; 302. movable interception block; 303. support column; 304. contact ball; 4. limit assembly; 401. connecting rod; 402. support block; 5. sleeve; 6. collecting rubber leather bag; 7. movable hole; 8. control rod; 9. pipe cap; 10. sealing rubber ring; 11. pressing block. DETAILED DESCRIPTION

[0033] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present disclosure. Instead, they are merely examples of devices consistent with some aspects of the present disclosure as detailed in the appended claims.

[0034] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0035] Embodiment 1:

[0036] like Figures 1 to 7 As shown, this embodiment proposes a cell centrifuge collector, including a bottom tube 1, a connecting tube 2, an interception component 3, a limit component 4, a sleeve 5, a collecting rubber leather bag 6, a movable hole 7, a control rod 8, a tube cap 9 and a pressing block 11 arranged at the lower end of the control rod 8.

[0037] The lower end of the bottom tube 1 is tapered, and a groove is provided at the position of the variable diameter, so that the sleeve 5 on the lower side can be sleeved on the bottom. The upper end of the bottom tube 1 is open, and a connecting tube 2 which is open both up and down is provided at the upper end of the bottom tube 1. The inner lower side of the connecting tube 2 and the outer upper side of the bottom tube 1 are threadedly connected together, so that the bottom tube 1 and the connecting tube 2 can be connected and the two tubes can be disassembled. A collecting rubber leather bag 6 which is also tapered and fits the inner lower end of the bottom tube 1 under normal circumstances is provided at the inner lower side of the bottom tube 1. After centrifugation, the animal cells agglomerate and fall on the bottom tube 1. The bottom of the collecting rubber leather bag 6 is used for collecting the bottom, the edge of the outer upper end of the collecting rubber leather bag 6 is fixedly connected to the inner wall of the inner lower side of the bottom tube 1, and a control rod 8 is fixedly connected to the outer lower end of the collecting rubber leather bag 6. The lower end of the control rod 8 extends to the outer lower side of the bottom tube 1 through the movable hole 7 opened on the outer lower side of the bottom tube 1, and a pressing block 11 is fixedly connected to the lower end of the control rod 8. The control rod 8 can be used to push the middle of the collecting rubber leather bag 6 upward to deform the collecting rubber leather bag 6, so that the animal cell clusters collected therein can be pushed out of the bottom tube 1.

[0038] After the cells are centrifuged, the upper clarified liquid needs to be poured out. A clamping ring 301 is provided at the upper end of the bottom tube 1 on the lower inner side of the connecting tube 2 after the bottom tube 1 and the connecting tube 2 are tightened. The upper outer side of the clamping ring 301 is an inwardly inclined slope. In order to allow the animal cell agglomerates to slide down along the slope and into the bottom tube 1 during centrifugation and to prevent the clamping ring 301 from blocking the animal cell agglomerates from sliding down, an umbrella-shaped movable interception block 302 is provided inside the bottom tube 1. The outer diameter of the movable interception block 302 is smaller than the inner diameter of the bottom tube 1, but larger than the clamping ring 301. The inner diameter is large, so when the bottom tube 1 and the connecting tube 2 are connected together, the movable intercepting block 302 can block the inside of the clamping ring 301 upward, and when the movable intercepting block 302 is on the outer lower side of the clamping ring 301, the animal cell clusters can fall along the gap between the movable intercepting block 302 and the bottom tube 1, and the movable intercepting block 302 is umbrella-shaped, which reduces the obstruction to the animal cell clusters. The sealing rubber ring 10 is fixedly connected to the outer lower side of the clamping ring 301 to ensure the sealing of the connection between the bottom tube 1 and the connecting tube 2. At the same time, the pipe cap 9 threadedly connected to the outer upper end of the connecting tube 1 can also be screwed on the bottom tube 1.

[0039] Embodiment 2:

[0040] The solution in Example 1 is further introduced below in combination with a specific working method, as described below:

[0041] In order to facilitate the control of the up and down movement of the movable intercepting block 302, a support column 303 is fixedly connected to the outer lower side of the movable intercepting block 302, and a contact ball 304 is fixedly connected to the lower end of the support column 303. The contact ball 304 contacts the inner bottom end of the collecting rubber leather bag 6, and the movable intercepting block 302 is controlled to move upward by pushing up the middle of the collecting rubber leather bag 6, blocking the middle of the clamping ring 301, and then pouring out the clarified liquid on the upper side, leaving the animal cell agglomerates inside the bottom tube 1.

[0042] After pouring out the clarified liquid, use a pipette to remove the small amount of clarified liquid remaining in the bottom tube 1, and pour out the animal cell agglomerates collected by centrifugation from the bottom tube 1. When pouring, first take out the movable intercepting block 302, turn the bottom tube 1 upside down, and pour out the animal cell agglomerates collected in the rubber bag 6 by inertia through bouncing or quickly pressing the pressing block 11.

[0043] Embodiment 3:

[0044] The solution in Example 2 is further introduced below in combination with a specific working method, as described below:

[0045] In order to ensure the smooth up and down movement of the movable intercepting block 302, three connecting rods 401 are fixedly connected around the support column 303 connected to the lower end of the movable intercepting block 302, and the other end of the connecting rod 401 connected to the support column 303 is fixedly connected to an arc-shaped support block 402, and the side of the support block 402 away from the connecting rod 401 is in contact with the inner wall of the bottom tube 1. The friction between the support block 402 and the inner wall of the bottom tube 1 can prevent the movable intercepting block 302 from moving up and down at will during the centrifugation process, ensuring that the contact ball 304 is on the lower side of the collecting rubber leather bag 6, so as to avoid excessive squeezing of the animal cell agglomerates collected inside the collecting rubber leather bag 6 by the contact ball 304 when the movable intercepting block 302 is pressed upward after the centrifugation is completed.

[0046] At the same time, after pushing the movable intercepting block 302 upwards and pouring out the clarified liquid inside the connecting tube 2, the bottom tube 1 and the connecting tube 2 are disassembled, and the upper end of the movable intercepting block 302 can expose a part of the interior of the bottom tube 1, so that the movable intercepting block 302, the support column 303, the contact ball 304, the connecting rod 401 and the support block 402 can be conveniently removed from the interior of the bottom tube 1, and the bottom tube 1 can be inverted to collect the animal cell clusters after centrifugation of the sub-tubes.

[0047] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell centrifugal collector, characterized in that: include: A bottom tube (1), the bottom end of the bottom tube (1) is tapered, a collecting rubber leather bag (6) is provided on the lower side of the bottom tube (1), the upper edge of the outer side of the collecting rubber leather bag (6) is fixedly connected to the inner wall of the bottom tube (1), a movable hole (7) is provided on the lower side of the outer side of the bottom tube (1), a control rod (8) is provided inside the movable hole (7), and the upper end of the control rod (8) is fixedly connected to the lower side of the outer side of the collecting rubber leather bag (6); A connecting pipe (2), the connecting pipe (2) being arranged on the upper side of the outside of the bottom pipe (1), the lower end of the inside of the connecting pipe (2) being threadedly connected to the upper end of the outside of the bottom pipe (1), a pipe cap (9) being arranged on the upper side of the outside of the connecting pipe (2), the lower end of the inside of the pipe cap (9) being threadedly connected to the upper end of the outside of the connecting pipe (2); An interception assembly (3), the interception assembly (3) being arranged between the bottom tube (1) and the connecting tube (2), the interception assembly (3) comprising a clamping ring (301), a movable interception block (302), a support column (303) and a contact ball (304), the clamping ring (301) being arranged at the lower side of the inside of the connecting tube (2), the outer edge of the clamping ring (301) being fixedly connected to the inner wall of the connecting tube (2), the movable interception block (302) being arranged at the inside of the bottom tube (1), the support column (303) being fixedly connected to the lower side of the movable interception block (302), the contact ball (304) being fixedly connected to the lower end of the support column (303), and the contact ball (304) being adapted to the inside of the collecting rubber bag (6); A limiting assembly (4), wherein the limiting assembly (4) is arranged between the support column (303) and the bottom tube (1); The sleeve (5) is arranged on the lower side of the outside of the bottom tube (1), the sleeve (5) is sleeved on the lower side of the outside of the bottom tube (1), and the control rod (8) is matched with the inside of the sleeve (5).

2. A cell centrifugal collector according to claim 1, characterized in that: The outer upper surface of the clamp ring (301) is inclined inwards, the movable intercepting block (302) is umbrella-shaped, and a sealing rubber ring (10) used in conjunction with the bottom tube (1) is fixedly connected to the outer lower side of the clamp ring (301).

3. A cell centrifugal collector according to claim 1, characterized in that: The outer diameter of the movable intercepting block (302) is smaller than the inner diameter of the bottom pipe (1), and the outer diameter of the movable intercepting block (302) is larger than the inner diameter of the clamping ring (301).

4. A cell centrifugal collector according to claim 1, characterized in that: The lower end of the control rod (8) is fixedly connected to a pressing block (11) on the lower side of the outside of the bottom tube (1), and the control rod (8) is slidably connected to the inside of the movable hole (7).

5. A cell centrifugal collector according to claim 1, characterized in that: The limiting component (4) comprises: Connecting rods (401), wherein a plurality of connecting rods (401) are evenly distributed and fixedly connected to the outside of the supporting column (303); A support block (402), wherein a plurality of the support blocks (402) are evenly distributed inside the bottom tube (1), the support block (402) is fixedly connected to the other end of the connecting rod (401) connected to the support column (303), and the side of the support block (402) away from the connecting rod (401) is slidably connected to the inner wall of the bottom tube (1).

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