Kidney primary tissue cell extraction device

Abstract

The kidney primary tissue cell extraction device comprises a crushing mechanism and an extraction mechanism located below the crushing mechanism, and the output end of the crushing mechanism is connected with the input end of the extraction mechanism through a connecting pipe; the crushing mechanism comprises a crushing tank, a rotating shaft and a crushing cutter, one end of the rotating shaft extends into the crushing tank, the crushing cutter is arranged in the crushing tank and connected with the rotating shaft, an output port is formed in the output end of the crushing tank, a filter screen is arranged at the output port, and the crushing cutter is located above the filter screen; the extraction mechanism comprises an extraction tank; and a switch valve is arranged on the connecting pipe. According to the principle of the utility model, the kidney primary tissue is put into the crushing tank, and the crushing knife is driven by the rotating shaft to crush and separate the tissue; after the crushed and separated tissues meet the diameter requirement, the tissues enter the extraction tank through the filter screen and the connecting pipe, and the tissues with the diameters not meeting the requirement continue to be separated in the crushing tank. According to the utility model, two steps of crushing separation and dissociation extraction are integrated together, so that the extraction efficiency and the cell viability are improved, and the risk that tissues are polluted is reduced.

Description

Technical Field

[0001] The utility model relates to an experimental apparatus, in particular to a kidney primary tissue cell extraction device. Background Art

[0002] Primary tissue cell extraction experiments generally include two steps: tissue fragmentation and dissociation extraction. Currently, the tissue fragmentation step and the dissociation extraction step are performed in different experimental instruments, and the tissue needs to be transferred during the experiment, which is not only inefficient but also has the risk of tissue contamination. Summary of the invention

[0003] The technical problem to be solved by the utility model is to provide a device for extracting primary kidney tissue cells, thereby improving efficiency and reducing the risk of tissue contamination.

[0004] In order to solve the above technical problems, the technical solution of the utility model is: a primary kidney tissue cell extraction device, including a crushing mechanism and an extraction mechanism located below the crushing mechanism, the output end of the crushing mechanism is connected to the input end of the extraction mechanism through a connecting pipe; the crushing mechanism includes a crushing tank, a rotating shaft with one end extending into the crushing tank, and a crushing knife arranged in the crushing tank and connected to the rotating shaft, the output end of the crushing tank is provided with an output port, a filter is provided at the output port, and the crushing knife is located above the filter; the extraction mechanism includes an extraction tank; and a switch valve is provided on the connecting pipe. Principle of the utility model: put the primary kidney tissue into the crushing tank, and drive the crushing knife to crush and separate the tissue by the rotating shaft; the crushed and separated tissue meets the diameter requirement and enters the extraction tank through the filter and the connecting pipe, and the tissue that does not meet the diameter requirement continues to stay in the crushing tank for separation. The utility model integrates the two steps of crushing and separation and dissociation extraction, improves the tissue dissociation efficiency and cell activity, and reduces the risk of tissue contamination.

[0005] As an improvement, the crushing tank includes a tank body with an open upper end and a cover body cooperating with the tank body, the lower end of the rotating shaft passes through the cover body and extends into the tank body, the upper end of the rotating shaft extends out of the cover body and can be connected to a manual drive mechanism or an electric drive mechanism.

[0006] As an improvement, the crushing mechanism, the extraction mechanism and the connecting pipe are fixed on the bracket.

[0007] As an improvement, the crushing knife includes a plurality of blades, and a plurality of teeth are arranged on the blade edges of the blades.

[0008] As an improvement, the bottom of the crushing tank is conical.

[0009] Compared with the prior art, the utility model has the following beneficial effects:

[0010] The utility model integrates the two steps of crushing separation and dissociation extraction, thereby improving tissue dissociation efficiency and cell activity and reducing the risk of tissue contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a schematic diagram of the utility model.

[0012] Figure 2 Schematic diagram of the crushing knife. DETAILED DESCRIPTION

[0013] The utility model will be further described below in conjunction with the accompanying drawings.

[0014] like Figure 1 As shown, a kidney primary tissue extraction device includes a crushing mechanism 1 and an extraction mechanism 2 located below the crushing mechanism 1, the output end of the crushing mechanism 1 is connected to the input end of the extraction mechanism 2 through a connecting tube 3, the crushing mechanism 1, the extraction mechanism 2 and the connecting tube 3 are fixed on a bracket, and the crushing mechanism 1 and the extraction mechanism 2 can be separated and taken out.

[0015] like Figure 1 As shown, the crushing mechanism 1 includes a crushing tank 11, a rotating shaft 12 with one end extending into the crushing tank 11, and a crushing knife 13 arranged in the crushing tank 11 and connected to the rotating shaft 12; the crushing tank 11 includes a tank body with an upper end opening and a cover body matched with the tank body, the cover body and the tank body are movably matched, and it is convenient to clean the inside of the tank body after opening the cover body; the lower end of the rotating shaft 12 passes through the cover body and extends into the tank body, the upper end of the rotating shaft 12 extends on the cover body, and the rotating shaft 12 is pivotally connected to the bearing seat on the cover body; the upper end of the rotating shaft 12 forms a connection on the cover body The connector can be connected to a hand-cranked wheel or a motor 15 to achieve manual or electric crushing; the bottom of the crushing tank 11 is conical, which has a convergence effect and can guide the crushed and separated tissue into the connecting pipe 3; an output port is provided at the output end at the bottom of the crushing tank 11, and a filter screen 14 is provided at the output port. The crushing knife 13 is located above the filter screen 14, and the tissue particles crushed and separated by the crushing knife 13 enter the output port through the filter screen 14. The tissue particles can pass through the filter screen 14 by their own weight or the pressure given by the crushing knife 13; Figure 2 As shown, the crushing knife 13 includes a plurality of blades 131, and a plurality of teeth 132 are arranged on the blade edge of the blade 131, which can quickly crush the tissue and separate particles with smaller diameters. The extraction mechanism 2 includes an extraction tank 21, and a filling port and a discharge port can be provided on the extraction tank 21 to facilitate the addition of various extraction solutions. The connecting pipe 3 is provided with a switch valve 31, and the switch valve 31 can be closed first during crushing, and the switch valve 131 can be opened after the crushing is completed to introduce the crushed tissue into the extraction tank 21.

[0016] The principle of the utility model is as follows: put the primary kidney tissue into the crushing tank 11, and drive the crushing knife 13 through the rotating shaft 12 to crush and separate the tissue; after the crushed and separated tissue meets the diameter requirement, it passes through the filter 14 and the connecting pipe 3 and enters the extraction tank 21, and the tissue that does not meet the diameter requirement continues to stay in the crushing tank 11 for separation. The utility model integrates the two steps of crushing and separation and dissolution and extraction, improves the tissue dissociation efficiency and cell activity, and reduces the risk of tissue contamination.

Claims

1. A device for extracting primary kidney tissue cells, characterized in that: It includes a crushing mechanism and an extraction mechanism located below the crushing mechanism, wherein the output end of the crushing mechanism is connected to the input end of the extraction mechanism through a connecting pipe; the crushing mechanism includes a crushing tank, a rotating shaft with one end extending into the crushing tank, and a crushing knife arranged in the crushing tank and connected to the rotating shaft, an output port is provided at the output end of the crushing tank, a filter is provided at the output port, and the crushing knife is located above the filter; the extraction mechanism includes an extraction tank; and a switch valve is provided on the connecting pipe.

2. A kidney primary tissue cell extraction device according to claim 1, characterized in that: The crushing tank comprises a tank body with an upper opening and a cover body matched with the tank body, the lower end of the rotating shaft passes through the cover body and extends into the tank body, and the upper end of the rotating shaft extends out of the cover body and can be connected to a manual drive mechanism or an electric drive mechanism.

3. The device for extracting primary kidney tissue cells according to claim 1, characterized in that: The crushing mechanism, the extraction mechanism and the connecting pipe are fixed on the bracket.

4. The device for extracting primary kidney tissue cells according to claim 1, characterized in that: The crushing knife comprises a plurality of blades, and a plurality of blade teeth are arranged on the blade edges of the blades.

5. The device for extracting primary kidney tissue cells according to claim 1, characterized in that: The bottom of the crushing tank is conical.

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