Fixed bed bioreactor sampling structure
By designing the sampling structure of multiple sampling seats and hose systems in the fixed bed bioreactor, the existing sampling methods are inconvenient and contaminated, convenient and efficient sampling operations are achieved, and the cell culture environment is maintained stable and sterile.
Patent Information
- Application Number
- CN202421336270.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-12
- Publication Date
- 2025-04-25
- Estimated Expiration
- 2034-06-12
AI Technical Summary
The existing fixed bed bioreactor sampling method is inconvenient, it is easy to destroy the cell culture environment, and external gases are easily entered into the tank, causing contamination.
A fixed bed bioreactor sampling structure is designed, using multiple sampling seats and hose systems, one end of the sampling strip is placed outside the hose, and the opening and closing of the hose is controlled by pipe clamps to achieve convenient sampling operations and suppress the entry of external gas through a micro positive pressure environment.
It improves sampling efficiency, simplifies operations, reduces the damage to the cell culture environment, and effectively prevents external gas from entering the tank, maintaining the stability and sterility of the internal environment.
Smart Images
Figure CN222795595U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of fixed bed bioreactors, and in particular to a fixed bed bioreactor sampling structure. Background Art
[0002] Fixed bed bioreactor is a new type of cell culture device. Compared with traditional bioreactors, it can simplify the culture process of adherent cells and is easy to scale up. The fixed bed bioreactor includes a tank body filled with culture fluid and a roll film arranged inside the tank body. During the cell culture stage, it is necessary to sample and analyze the cells attached to the roll film in real time.
[0003] At present, the existing method of collecting samples from the fixed bed is to use tools, such as tweezers, to reach into the bioreactor for sampling during operation, which is inconvenient to operate and easily causes harmful damage to the fixed bed cell culture environment, causing internal contamination of the bioreactor; other sampling methods are to set a sampling seat on the top of the tank body, and a cover is threadedly connected to the sampling seat, and the inner side of the cover is connected to a sampling strip placed inside the tank body. When sampling, the sampling strip is taken out by screwing the cover, which is time-consuming and laborious, and when sampling, external gas is easy to enter the tank body from the sampling seat, thereby destroying the cell culture environment inside the tank body. Utility Model Content
[0004] In order to improve the problem that the existing fixed bed bioreactor sampling method is inconvenient and easily causes internal contamination of the bioreactor, the present application provides a fixed bed bioreactor sampling structure.
[0005] The present application provides a fixed bed bioreactor sampling structure, which adopts the following technical solution:
[0006] A fixed-bed bioreactor sampling structure comprises a tank body, a film roll is arranged inside the tank body, a plurality of sampling seats are connected through the top of the tank body, and a hose is connected to one end of the sampling seat located outside the tank body; a sampling strip is passed through the hose, one end of the sampling strip is placed outside the hose, and the other end extends along the hose to the film roll in the tank body; a tube control for clamping or loosening the hose is arranged on the hose.
[0007] By adopting the above technical solution and setting up multiple sampling seats, sampling can be achieved at different positions on the roll film, and the roll film can be sampled in batches. By placing one end of the sampling strip outside the hose and using the tube clamp to control the opening and closing of the hose, when sampling, you only need to open the tube clamp, pull the end of the sampling strip outside the hose, and take the sampling strip out along the hose. The operation is simple and convenient, which improves the sampling efficiency and reduces the impact of the external environment on the internal environment of the tank. After sampling, the hose is clamped again by the tube clamp to prevent external gas from entering the tank and causing pollution. Since the tank is slightly positive pressure, external gas can be suppressed from entering the tank during sampling to ensure that the internal environment of the tank is not damaged. The tank is always maintained in a slightly positive pressure state by a filter air pump, and the gas entering the tank is ensured to be sterile by filtering.
[0008] Optionally, a connecting portion for connecting a hose is provided at one end of the sampling seat, an anti-slip protrusion is provided on the connecting portion, one end of the hose is sleeved on the connecting portion, and the anti-slip protrusion is pressed against the inner wall of the hose.
[0009] By adopting the above technical solution, since the anti-slip protrusion is provided on the connecting portion, the connection of the hose sleeved on the connecting portion is made more stable, and the sealing between the hose and the connecting portion is improved.
[0010] Optionally, the diameter of the anti-slip protrusion at one end close to the hose is smaller than the diameter of the anti-slip protrusion at one end away from the hose.
[0011] By adopting the above technical solution, the hose can be easily installed on the connecting part, and the stability of the connection between the hose and the connecting part is increased, so that the hose is not easy to fall off.
[0012] Optionally, a limiting ring is provided on the sampling seat, and one side of the limiting ring abuts against the outer side of the top of the tank body; an end of the sampling seat away from the connecting portion is sleeved with a sealing sleeve, and one end of the sealing sleeve abuts against the inner side of the top of the tank body.
[0013] By adopting the above technical solution, the sampling seat is fixed on the top of the tank body through the cooperation of the limiting ring and the sealing sleeve, and the sealing sleeve ensures the sealing of the connection between the sampling seat and the top of the tank body.
[0014] Optionally, a sealing ring is provided between the limiting ring and the tank body, a placement groove for the sealing ring to be inserted is provided on a side of the limiting ring close to the tank body, and one side of the sealing ring abuts against the tank body.
[0015] By adopting the above technical solution, a sealing ring is arranged between the limiting ring and the tank body to ensure the sealing between the limiting ring and the tank body, thereby avoiding the influence of the external environment on the internal environment of the tank body.
[0016] Optionally, a mounting seat is provided on the inner side of the top of the tank body, a slot for the sealing sleeve to be inserted is provided in the mounting seat, and the outer peripheral wall of the sealing sleeve is pressed against the inner wall of the slot.
[0017] By adopting the above technical solution, the sealing sleeve is clamped in the groove of the mounting seat, thereby ensuring the connection stability of the sealing sleeve and improving the sealing performance of the connection between the sealing sleeve and the mounting seat.
[0018] Optionally, a support frame for fixing the film roll is provided in the tank body, a plurality of sampling columns are provided at the top of the support frame, the sampling columns correspond to the positions of the sampling seats one by one, and the sampling strips are inserted in the sampling columns.
[0019] By adopting the above technical solution, stirring is required during the experiment. The sampling bars are fixed in position by arranging sampling columns on the support frame to avoid entanglement between the sampling bars and affect the sampling results.
[0020] Optionally, the tube control is a tube clamp or a cable tie.
[0021] By adopting the above technical solution, the clamping and loosening of the hose is controlled by using a cable tie or a pipe clamp, thereby ensuring the sealing of the hose and facilitating subsequent sampling.
[0022] Optionally, one end of the hose away from the sampling seat is oriented downward.
[0023] By adopting the above technical solution, the dust in the air is reduced from falling into the hose, and the influence on the sampling strip during sampling is reduced.
[0024] In summary, the present application includes at least one of the following beneficial technical effects: by placing one end of the sampling strip outside the hose and using the tube clamp to control the opening and closing of the hose; when sampling, by opening the tube clamp, pulling the end of the sampling strip outside the hose, directly taking out the sampling strip, and then clamping the tube clamp to complete the sampling. This sampling method is simple and convenient to operate. At the same time, the inside of the tank body is slightly positive pressure, which can inhibit external gas from entering the inside of the tank body during sampling, thereby ensuring that the internal environment of the tank body is not damaged. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a structural schematic diagram of the sampling structure of the fixed bed bioreactor of the present application.
[0026] Figure 2 It is a cross-sectional view of the sampling structure of the fixed bed bioreactor of the present application.
[0027] Figure 3 It is a schematic diagram of the structure of the sampling seat of this application.
[0028] Figure numerals: 1, tank body; 11, mounting seat; 111, card slot; 2, roll film; 3, sampling seat; 31, connecting part; 311, anti-slip protrusion; 32, limiting ring; 321, placement groove; 33, sealing ring; 34, sealing sleeve; 35, channel; 4, hose; 5, sampling strip; 6, air inlet; 7, tube control unit; 71, tube clamp; 72, cable tie; 8, support frame; 9, sampling column. DETAILED DESCRIPTION
[0029] Embodiments of the present application are described in detail below, examples of which are shown in the accompanying drawings.
[0030] In the description of this specification, the description with reference to the terms "certain embodiments", "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiments or examples are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.
[0031] The present application embodiment discloses a fixed bed bioreactor sampling structure, referring to Figure 1 and Figure 2 , comprising a tank body 1, a roll film 2 is fixedly connected inside the tank body 1, a plurality of sampling seats 3 are connected through the top of the tank body 1, and the plurality of sampling seats 3 are evenly distributed on one side of the top of the tank body along the circumference of the tank body 1, and one end of the sampling seat 3 located outside the tank body 1 is connected to a hose 4; a sampling strip 5 is passed through the hose 4, and the sampling strip 5 is preferably made of a PET film of food and medicine grade, one end of the sampling strip 5 is placed outside the hose 4, and the other end extends along the hose 4 to the roll film 2 in the tank body 1; a hose clamp 71 for clamping or loosening the hose 4 is provided on the hose 4.
[0032] By setting a plurality of sampling seats 3, sampling can be achieved at different positions on the roll film 2, and the roll film 2 can be sampled in batches. By placing one end of the sampling strip 5 outside the hose 4 and using the tube clamp 71 to control the opening and closing of the hose 4, when sampling, it is only necessary to open the tube clamp 71, pull the end of the sampling strip 5 outside the hose 4, and take the sampling strip 5 out along the hose 4. The operation is simple and convenient, the sampling efficiency is improved, and the influence of the external environment on the internal environment of the tank body 1 is reduced; after the sampling is completed, the hose 4 is clamped again by the tube clamp 71 to prevent external gas from entering the tank and causing pollution.
[0033] The hose 4 preferably adopts a food and medicine grade silicone hose, and can also adopt a polyvinyl chloride hose, a polyurethane hose, a PET transparent hose, etc.
[0034] The top of the tank body 1 is connected with an air inlet 6, which is used to maintain a slight positive pressure inside the tank body 1. The air inlet 6 is connected to an external filter air pump. When taking out samples, since the tank body 1 is slightly positively pressurized, external gas can be prevented from entering the tank body 1, ensuring the stability of the internal environment of the tank body 1; the air inlet 6 is connected to the filter air pump, so that the tank body 1 is always maintained in a slightly positive pressure state, and the gas entering the tank is ensured to be sterile through filtration.
[0035] A support frame 8 for fixing the film roll 2 is provided in the tank body 1. The film roll 2 is wound on the support frame 8. A plurality of sampling columns 9 are connected to the top of the support frame 8. The sampling columns 9 are hollow tubular structures. The positions of the sampling columns 9 and the sampling seats 3 correspond one to one. One end of the sampling strip 5 is inserted into the sampling column 9. When conducting an experiment, stirring is required. By setting the sampling column 9 on the support frame 8 to fix the position of the sampling strip 5, entanglement between the sampling strips 5 is avoided to affect the sampling results.
[0036] The tube control 7 is a tube clamp 71 or a cable tie 72. The tube clamp 71 is clamped on the hose 4 to control the opening and closing of the hose 4, thereby ensuring the sealing of the inside of the hose 4 and facilitating the subsequent sampling operation. The cable tie 72 is a self-locking cable tie 72. When in use, the hose 4 is folded in half and tied with the cable tie 72 at the folded part of the hose 4 to ensure the sealing of the hose 4.
[0037] The direction of the end of the hose 4 away from the sampling seat 3 is downward, which reduces the dust in the air from falling into the hose 4 and reduces the influence on the sampling strip 5 during sampling.
[0038] Reference Figure 2 and Figure 3 The sampling seat 3 is provided with a passage 35 connected to the inside of the tank 1. A connection portion 31 for connecting the hose 4 is provided at one end of the sampling seat 3. The connection portion 31 is provided with an anti-skid protrusion 311. One end of the hose 4 is sleeved on the connection portion 31, so that when the anti-skid protrusion 311 is connected to the hose 4, the pressure between the connection portion 31 and the hose 4 is increased to achieve a seal between the connection portion 31 and the hose 4, and at the same time, the connection of the hose 4 is more stable. The anti-skid protrusion 311 is a conical structure. In order to facilitate the insertion of the hose 4, the diameter of the anti-skid protrusion 311 close to the hose 4 is smaller than the diameter of the anti-skid protrusion 311 away from the hose 4.
[0039] A limiting ring 32 is fixedly connected to the sampling seat 3, and one side of the limiting ring 32 abuts against the outer side of the top of the tank body 1; a sealing sleeve 34 is sleeved on one end of the sampling seat 3 away from the connecting portion 31, and the sealing sleeve 34 is preferably made of hard rubber material, and one end of the sealing sleeve 34 abuts against the inner side of the top of the tank body 1. The limiting ring 32 cooperates with the sealing sleeve 34 to fix the sampling seat 3 on the top of the tank body 1, and the sealing sleeve 34 ensures the sealing of the connection between the sampling seat 3 and the top of the tank body 1.
[0040] A sealing ring 33 is arranged between the limiting ring 32 and the tank body 1. A placement groove 321 for the sealing ring 33 to be inserted is provided on one side of the limiting ring 32 close to the tank body 1. The outer peripheral wall of the sealing ring 33 abuts against the inner wall of the placement groove 321, and one side of the sealing ring 33 abuts against the tank body 1. The sealing ring 33 is arranged between the limiting ring 32 and the tank body 1 to ensure the sealing between the limiting ring 32 and the tank body 1 and prevent the external environment from affecting the internal environment of the tank body 1.
[0041] The top inner side of the tank body 1 is fixedly connected with a mounting seat 11, which is integrally formed with the top of the tank body 1. A slot 111 is provided in the mounting seat 11 for the sealing sleeve 34 to be inserted into, and the outer peripheral wall of the sealing sleeve 34 is pressed against the inner wall of the slot 111. The sealing sleeve 34 is inserted into the slot of the mounting seat 11 to ensure the connection stability of the sealing sleeve 34 and improve the sealing performance of the connection between the sealing sleeve 34 and the mounting seat 11.
[0042] The implementation principle of the fixed bed bioreactor sampling structure of the present application embodiment is as follows: in the early preparation stage, the film roll 2 is first placed on the support frame 8 of the tank body 1, one end of the sampling strip 5 is placed in the film roll 2, and the other end passes through the sampling column 9, the sampling seat 3 and the hose 4 in sequence, and extends to the outside of the hose 4, and is clamped on the hose 4 with a tube clamp 71. When sampling, by opening the tube clamp 71, the end of the sampling strip 5 outside the hose 4 is pulled by a tool, the sampling strip 5 is directly taken out along the hose 4, and then the tube clamp 71 is clamped, so as to complete the sampling. This sampling method is simple and convenient to operate, and at the same time, the inside of the tank body 1 is slightly positively pressurized, which can inhibit external gas from entering the inside of the tank body 1 during sampling, ensuring that the internal environment of the tank body 1 is not damaged.
[0043] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.
Claims
1. A fixed bed bioreactor sampling structure, characterized in that: The invention comprises a plurality of sampling seats (3) penetrating and communicating with the top of a tank body (1), wherein one end of the sampling seats (3) located outside the tank body (1) is sealedly connected to a hose (4); a sampling strip (5) is passed through the hose (4), one end of the sampling strip (5) is placed outside the hose (4), and the other end extends along the hose (4) to a rolled film (2) in the tank body (1); a tube control (7) for clamping or loosening the hose (4) is provided on the hose (4); an air inlet (6) is penetrating through the top of the tank body (1), the air inlet (6) is used to maintain a slight positive pressure inside the tank body (1), and the air inlet (6) is connected to an external filtering air pump.
2. The fixed bed bioreactor sampling structure according to claim 1, characterized in that: One end of the sampling seat (3) is provided with a connecting portion (31) for connecting to a hose (4), and an anti-slip protrusion (311) is provided on the connecting portion (31). One end of the hose (4) is sleeved on the connecting portion (31), and the anti-slip protrusion (311) is pressed against the inner wall of the hose (4).
3. The fixed bed bioreactor sampling structure according to claim 2, characterized in that: The diameter of the anti-slip protrusion (311) at one end close to the hose (4) is smaller than the diameter of the anti-slip protrusion (311) at one end away from the hose (4).
4. The fixed bed bioreactor sampling structure according to claim 1, characterized in that: A limiting ring (32) is provided on the sampling seat (3), one side of the limiting ring (32) abuts against the outer side of the top of the tank body (1), and a sealing sleeve (34) is sleeved on the end of the sampling seat (3) away from the connecting portion (31), and one end of the sealing sleeve (34) abuts against the inner side of the top of the tank body (1).
5. The fixed bed bioreactor sampling structure according to claim 4, characterized in that: A sealing ring (33) is provided between the limiting ring (32) and the tank body (1); a placement groove (321) for the sealing ring (33) to be inserted is provided on a side of the limiting ring (32) close to the tank body (1); and one side of the sealing ring (33) abuts against the tank body (1).
6. The fixed bed bioreactor sampling structure according to claim 4, characterized in that: A mounting seat (11) is arranged on the inner side of the top of the tank body (1), and a slot (111) is provided in the mounting seat (11) for the sealing sleeve (34) to be inserted into, and the outer peripheral wall of the sealing sleeve (34) is pressed against the inner wall of the slot (111).
7. The fixed bed bioreactor sampling structure according to claim 1, characterized in that: A support frame (8) for fixing the roll film (2) is arranged in the tank body (1), and a plurality of sampling columns (9) are arranged at the top of the support frame (8). The positions of the sampling columns (9) and the sampling seats (3) correspond one to one, and the sampling strips (5) are inserted into the sampling columns (9).
8. The fixed bed bioreactor sampling structure according to claim 1, characterized in that: The pipe control part (7) is a pipe clamp (71) or a cable tie (72).
9. The fixed bed bioreactor sampling structure according to claim 1, characterized in that: The direction of the hose (4) away from one end of the sampling seat (3) is downward.