Industrial-scale bioreactor device capable of transferring heat and used for static culture
By using a temperature control system of annular coils and jacketed devices in the bioreactor, the problem of uneven temperature distribution and high energy consumption during the anaerobic fermentation process is solved, and a more efficient fermentation process and industrial scale production is achieved.
Patent Information
- Application Number
- CN202420899146.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-28
- Publication Date
- 2025-04-29
- Estimated Expiration
- 2034-04-28
AI Technical Summary
During the microbial anaerobic fermentation process, existing mechanical stirring fermentation tanks have problems such as the fermentation broth not being able to flow and mix and heat exchange, poor temperature distribution uniformity, and the central temperature cannot be accurately regulated, resulting in high energy consumption and low production capacity during the fermentation process, making it difficult to achieve large-scale industrial production.
An industrial-scale bioreactor device for stand-alive culture that can transfer heat was designed, using an annular coil device and a jacket device for temperature regulation, and the fermentation situation is monitored in real time through the glasses, and the flow rate of circulating water is adjusted to achieve uniform temperature distribution and precise temperature control of the fermentation broth.
It improves the overall heat transfer efficiency of the fermentation broth during the fermentation process, ensures that microorganisms grow and metabolize at appropriate temperatures, improves the anaerobic static fermentation efficiency and production capacity, and realizes large-scale industrial production.
Smart Images
Figure CN222809468U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of fermentation equipment, in particular to an industrial-scale bioreactor device capable of transferring heat and used for static culture. Background Art
[0002] With the development of science and technology, bio-fermentation technology is increasingly used in the food, pharmaceutical, chemical and other industries. The bio-fermentation tank is one of the most important equipment in bio-engineering. Its main function is to provide a suitable growth environment for microorganisms, so that microorganisms can grow and reproduce quickly and efficiently. Its performance and efficiency directly affect the effect and yield of bio-fermentation.
[0003] Traditional mechanical stirring fermentation tanks are widely used for aerobic fermentation because of their good mixing, heat and mass transfer environment, which is conducive to the rapid growth of microorganisms and high fermentation efficiency. However, for some anaerobic microorganisms that have special requirements for the growth environment or products, the shear force caused by the strong mass transfer during the stirring or aeration process has a certain inhibitory effect on the growth of microorganisms or has a strong destructive effect on the fermentation products of microorganisms. In this case, the microorganisms need to be subjected to anaerobic static fermentation. However, the anaerobic static fermentation of microorganisms in the prior art still mainly uses mechanical stirring fermentation tanks, which are carried out without ventilation and stirring. Utility Model Content
[0004] The technical problems to be solved by the utility model are:
[0005] Since the mechanical stirring fermentation tank in the prior art mainly controls the temperature of the fermentation process and the heat exchange temperature control by controlling the outer jacket of the mechanical stirring fermentation tank cylinder. However, when using the mechanical stirring fermentation tank for microbial anaerobic static fermentation, there are problems such as the fermentation liquid cannot flow, mix and exchange heat, and the temperature distribution uniformity of the fermentation liquid is poor; and there is a problem that the central temperature of the fermentation liquid cannot be accurately controlled, resulting in the fermentation liquid in the mechanical stirring fermentation tank being too low in the early stage of fermentation and too high in the middle and late stages of fermentation, which also leads to high energy consumption and low production capacity during the fermentation process, making it difficult to achieve industrial-scale production.
[0006] In view of the above problems existing in the prior art, the utility model provides an industrial-scale bioreactor device for static culture capable of heat transfer.
[0007] Specifically, the utility model provides the following technical solutions:
[0008] The utility model provides an industrial-scale bioreactor device for static culture capable of heat transfer, which is provided with a tank body, wherein the tank body comprises a tank top, a tank body and a tank bottom.
[0009] The tank top is provided with a sight glass and a manhole.
[0010] The tank body is provided with a temperature control part, which includes an annular coil device and a jacket device. The annular coil device is sleeved in the tank body to control the central temperature of the fermentation liquid in the tank body. The jacket device is arranged around the outside of the tank body to form a jacket layer to control the temperature of the fermentation liquid near the inner wall of the tank body.
[0011] The annular coil device includes a hot water / cold water circulating water outlet of the annular coil, an annular coil and a hot water / cold water circulating water inlet of the annular coil. The annular coil is located at the central position inside the tank body, and spirally extends to the outer side wall of the middle and upper part of the tank body to form the hot water / cold water circulating water outlet of the annular coil, and spirally extends to the outer side wall of the middle and lower part of the tank body to form the hot water / cold water circulating water inlet of the annular coil.
[0012] Preferably, the jacket device comprises a hot water / cold water circulating water inlet of the jacket, a jacket and a hot water / cold water circulating water outlet of the jacket, the hot water / cold water circulating water inlet of the jacket is fixedly connected to the outer wall of the middle and lower part of the jacket, and the hot water / cold water circulating water outlet of the jacket is fixedly connected to the outer wall of the middle and upper part of the jacket.
[0013] Preferably, the tank body is provided with a ventilation pipe, which is sleeved inside the tank body, with one end of the ventilation pipe extending outward through the inner wall of the tank body to the outer wall of the upper middle part of the tank body, and the other end of the ventilation pipe extending downward through the inner wall of the tank body to the bottom.
[0014] Preferably, a stirring device is provided at the bottom of the tank, and the stirring device comprises a stirrer and a stirring motor.
[0015] Preferably, the output end of the stirring motor is rigidly connected to the rotating shaft of the stirrer, and a stirring blade is fixedly connected to the rotating shaft of the stirrer.
[0016] Preferably, the tank bottom is provided with a discharge port and supporting legs.
[0017] Preferably, the tank body is made of stainless steel.
[0018] Beneficial effects of the utility model:
[0019] (1) The utility model provides an industrial-scale bioreactor device for static culture that can transfer heat. During anaerobic static fermentation of microorganisms, by providing a sight glass and two sets of temperature control devices, namely a ring coil device and a jacket device, the fermentation situation in the tank can be observed in real time through the sight glass, and the flow rate of circulating water can be adjusted through the two sets of temperature control devices, thereby solving the problem that the fermentation liquid cannot flow, mix and exchange heat, the temperature distribution uniformity of the fermentation liquid is poor, and the central temperature of the fermentation liquid cannot be accurately controlled, thereby improving the overall heat transfer efficiency of the fermentation liquid during the fermentation process, ensuring that the microorganisms grow and metabolize at a suitable temperature, improving the efficiency and production capacity of anaerobic static fermentation, and realizing industrial-scale production.
[0020] (2) The utility model provides an industrial-scale bioreactor device for static culture capable of heat transfer, the internal structure of the tank body of which is simple and has no dead corners for cleaning. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 Schematic diagram of an industrial-scale bioreactor apparatus for static culture capable of heat transfer according to Example 1.
[0022] Figure 2 This is a structural diagram of the annular coil device of Example 1.
[0023] The markings in the figure are as follows: 1-sight mirror, 2-vent pipe, 3-circulating water outlet of hot water / cold water of annular coil, 4-annular coil, 5-circulating water inlet of hot water / cold water of jacket, 6-discharging port, 7-support leg, 8-manhole, 9-tank body, 10-circulating water outlet of hot water / cold water of jacket, 11-jacket, 12-circulating water inlet of hot water / cold water of annular coil, 13-agitator, 14-agitation motor. DETAILED DESCRIPTION
[0024] In order to better understand the above technical solution, the technical solution of the utility model is clearly and completely explained in combination with the specific implementation method below. It should be noted that the content in the specific implementation method is only a specific implementation and explanation of the technical solution of the utility model, and should not be understood as limiting the protection scope of the utility model.
[0025] It should be noted that relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "include" and "comprise" are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0026] In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "inside", "outside", "top", "bottom", "end" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, and a specific orientation structure and operation, and therefore cannot be understood as a limitation on the present invention.
[0027] In a specific embodiment of the present invention, an industrial-scale bioreactor device for static culture capable of heat transfer is provided, which comprises a tank body 9, wherein the tank body 9 comprises a tank top, a tank body and a tank bottom.
[0028] The tank top is provided with a sight glass 1 and a manhole 8.
[0029] The tank body is provided with a temperature control part, which includes an annular coil device and a jacket device. The annular coil device is sleeved in the tank body to control the central temperature of the fermentation liquid in the tank body. The jacket device is arranged around the outside of the tank body to form a jacket layer to control the temperature of the fermentation liquid near the inner wall of the tank body.
[0030] The annular coil device includes a hot water / cold water circulating water outlet 3 of the annular coil, an annular coil 4 and a hot water / cold water circulating water inlet 12 of the annular coil. The annular coil 4 is located at the central position inside the tank body, and spirally extends to the outer side wall of the middle and upper part of the tank body to form the hot water / cold water circulating water outlet 3 of the annular coil, and spirally extends to the outer side wall of the middle and lower part of the tank body to form the hot water / cold water circulating water inlet 12 of the annular coil.
[0031] In another specific embodiment of the present invention, an industrial-scale bioreactor device for static culture capable of heat transfer is provided, which comprises a tank body 9, wherein the tank body 9 comprises a tank top, a tank body and a tank bottom.
[0032] The tank top is provided with a sight glass 1 and a manhole 8.
[0033] The tank body is provided with a temperature control part, which includes an annular coil device and a jacket device. The annular coil device is sleeved in the tank body to control the central temperature of the fermentation liquid in the tank body. The jacket device is arranged around the outside of the tank body to form a jacket layer to control the temperature of the fermentation liquid near the inner wall of the tank body.
[0034] The annular coil device is sleeved inside the tank body to control the central temperature of the fermentation liquid in the tank body. Figure 2 As shown, Figure 2The structure diagram of the annular coil device of Example 1, the annular coil device includes a hot water / cold water circulating water outlet 3 of the annular coil, an annular coil 4 and a hot water / cold water circulating water inlet 12 of the annular coil, the annular coil 4 is located at the central position in the tank body, and spirally extends to the outer side wall of the middle and upper part of the tank body to form the hot water / cold water circulating water outlet 3 of the annular coil, and spirally extends to the outer side wall of the middle and lower part of the tank body to form the hot water / cold water circulating water inlet 12 of the annular coil,
[0035] The jacket device is arranged around the outer periphery of the tank body to form a jacket layer, which is used to control the temperature of the fermentation liquid near the inner wall of the tank body. The jacket device includes a hot water / cold water circulating water inlet 5 of the jacket, a jacket 11 and a hot water / cold water circulating water outlet 10 of the jacket. The hot water / cold water circulating water inlet 5 of the jacket is fixedly connected to the outer wall of the middle and lower part of the jacket 11, and the hot water / cold water circulating water outlet 10 of the jacket is fixedly connected to the outer wall of the middle and upper part of the jacket 11.
[0036] In a preferred embodiment of the present invention, the can body is further provided with a ventilation pipe 2, which is sleeved inside the can body, with one end of the ventilation pipe extending outward through the inner wall of the can body to the outer wall of the middle and upper part of the can body, and the other end of the ventilation pipe extending downward through the inner wall of the can body to the bottom.
[0037] In a preferred embodiment of the present invention, the tank body 9 is made of stainless steel and has certain strength and corrosion resistance. The tank body 9 includes a tank top, a tank body and a tank bottom. It should be noted that the tank body can also be made of other materials such as titanium material or titanium alloy material, as long as it has certain strength and corrosion resistance.
[0038] In a preferred embodiment of the utility model, a stirring device is provided at the bottom of the tank, and the stirring device includes a stirrer 13 and a stirring motor 14; more preferably, the output end of the stirring motor is rigidly connected to the rotating shaft of the stirrer, and two stirring blades are fixedly connected to the rotating shaft of the stirrer.
[0039] In a preferred embodiment of the present invention, a discharge port 6 and supporting legs 7 are further provided at the bottom of the tank.
[0040] Among them, in any of the above specific embodiments or preferred embodiments, the functions of the various parts of the industrial-scale bioreactor device for static culture capable of heat transfer are as follows:
[0041] Function of sight glass 1: to understand the fermentation status in the tank in real time and intuitively.
[0042] The function of the vent pipe 2 is to serve as a passage for steam to enter the can body, thereby allowing the steam to sterilize the inside of the can body.
[0043] The functions of the annular coil 4, the hot water / cold water circulating water outlet 3 of the annular coil and the hot water / cold water circulating water inlet 12 of the annular coil are as follows: serving as a circulation path for the hot water / cold water circulating water, for controlling the flow rate of the hot water / cold water circulating water, and regulating the central temperature of the fermentation liquid in the tank body.
[0044] The functions of the jacket 11, the hot water / cold water circulating water inlet 5 of the jacket and the hot water / cold water circulating water outlet 10 of the jacket are as follows: as a flow path for the hot water / cold water circulating water, used to control the flow rate of the hot water / cold water circulating water and regulate the temperature of the fermentation liquid near the inner wall of the tank body.
[0045] The function of the discharge port 6 is to serve as a channel for discharging the fermented liquid from the tank body.
[0046] The function of the legs 7 is to support and fix the tank body.
[0047] The manhole 8 serves as a passage for adding materials into the tank body and as a passage for maintenance and overhaul of the industrial-scale bioreactor device.
[0048] The function of the agitator 13 is to mix the liquid in the tank.
[0049] The stirring motor 14 functions as a driver to drive the stirrer to rotate.
[0050] The present invention is further described below in conjunction with the embodiments. It should be noted that the following embodiments are only more specific implementations and explanations of the technical solutions of the present invention and should not be construed as limiting the scope of protection of the present invention.
[0051] Embodiment 1:
[0052] The utility model provides an industrial-scale bioreactor device for static culture capable of heat transfer, comprising Figure 1 and Figure 2 As shown, it has a tank body 9, and the tank body 9 includes a tank top, a tank body and a tank bottom.
[0053] The tank top is provided with a sight glass 1 and a manhole 8.
[0054] The tank body is provided with a temperature control part, which includes an annular coil device and a jacket device. The annular coil device is sleeved in the tank body to control the central temperature of the fermentation liquid in the tank body. The jacket device is arranged around the outside of the tank body to form a jacket layer to control the temperature of the fermentation liquid near the inner wall of the tank body.
[0055] The annular coil device includes a hot water / cold water circulating water outlet 3 of the annular coil, an annular coil 4 and a hot water / cold water circulating water inlet 12 of the annular coil. The annular coil 4 is located at the central position inside the tank body, and spirally extends to the outer side wall of the middle and upper part of the tank body to form the hot water / cold water circulating water outlet 3 of the annular coil, and spirally extends to the outer side wall of the middle and lower part of the tank body to form the hot water / cold water circulating water inlet 12 of the annular coil.
[0056] Example 2
[0057] The utility model provides an industrial-scale bioreactor device for static culture capable of heat transfer, such as Figure 1 and Figure 2 As shown, it has a tank body 9, and the tank body 9 includes a tank top, a tank body and a tank bottom.
[0058] The tank body 9 is made of stainless steel and has certain strength and corrosion resistance. The tank body 9 includes a tank top, a tank body and a tank bottom. It should be noted that the tank body can also be made of other materials such as titanium material or titanium alloy material, as long as it has certain strength and corrosion resistance.
[0059] The tank top is provided with a sight glass 1 and a manhole 8.
[0060] The tank body is provided with a ventilation pipe 2 and a temperature control part, and the temperature control part includes an annular coil device and a jacket device.
[0061] The vent pipe 2 is sleeved in the tank body, one end of the vent pipe passes through the inner wall of the tank body and extends outward to the outer wall of the upper middle part of the tank body, and the other end of the vent pipe passes through the inner wall of the tank body and extends downward to the bottom.
[0062] The annular coil device is sleeved inside the tank body to control the central temperature of the fermentation liquid in the tank body. Figure 2 As shown, Figure 2 This is a structural diagram of the annular coil device of Example 1, wherein the annular coil device includes a hot water / cold water circulating water outlet 3 of the annular coil, an annular coil 4 and a hot water / cold water circulating water inlet 12 of the annular coil, wherein the annular coil 4 is located at the central position inside the tank body, and spirally extends to the outer side wall of the middle and upper part of the tank body to form the hot water / cold water circulating water outlet 3 of the annular coil, and spirally extends to the outer side wall of the middle and lower part of the tank body to form the hot water / cold water circulating water inlet 12 of the annular coil.
[0063] Preferably, in the utility model device, the jacket device can be arranged around the outer periphery of the tank body to form a jacket layer to regulate the temperature of the fermentation liquid near the inner wall of the tank body. The jacket device includes a hot water / cold water circulating water inlet 5 of the jacket, a jacket 11 and a hot water / cold water circulating water outlet 10 of the jacket, the hot water / cold water circulating water inlet 5 of the jacket is fixedly connected to the outer wall of the middle and lower part of the jacket 11, and the hot water / cold water circulating water outlet 10 of the jacket is fixedly connected to the outer wall of the middle and upper part of the jacket 11.
[0064] In addition, a stirring device may be provided at the bottom of the tank, and the stirring device includes a stirrer 13 and a stirring motor 14. In a specific embodiment, the output end of the stirring motor is rigidly connected to the rotating shaft of the stirrer, and two stirring blades are fixedly connected to the rotating shaft of the stirrer, and a discharge port 6 and a support leg 7 may also be provided at the bottom of the tank.
[0065] The working principle of the utility model is as follows:
[0066] (1) Material-liquid mixing stage: the material-liquid is added into the tank body through the manhole 8, and the stirring motor 14 is started at the same time. The stirring motor 14 drives the rotating shaft of the stirrer to rotate. The rotating shaft of the stirrer drives the stirring blades to stir the material-liquid in the tank body, so that the material-liquid in the tank body is fully and evenly mixed, that is, the material-liquid mixing is completed.
[0067] (2) Sterilization stage: hot water is introduced into the annular coil 4 through the hot water / cold water inlet 12 of the annular coil, and hot water is introduced into the jacket 11 through the hot water / cold water inlet 5 of the jacket to heat the temperature of the liquid in the tank to about 80°C. Then, steam is introduced into the tank through the vent pipe 2 to make the pressure and temperature in the tank reach the actual sterilization requirements and maintain them for a specific time. Then, the introduction of steam is stopped, and the hot water circulating water introduced into the annular coil device and the jacket device is switched to cold water circulating water for cooling, so that the temperature of the liquid is reduced to the fermentation temperature, and sterilization is completed.
[0068] (3) Fermentation stage: After the sterilization is completed, the fermentation bacteria are inoculated in the tank body. After the inoculation of the fermentation bacteria is completed, the stirring motor is turned off for static fermentation culture. Since the fermentation bacteria itself generates a certain amount of heat during the production and metabolism process, the temperature of the fermentation liquid in the tank body increases. By controlling the circulating water flow of hot water / cold water in the annular coil device and the jacket device, the fermentation liquid is maintained at the optimal fermentation temperature. When the fermentation is completed, the fermented liquid is discharged through the discharge port 6, and the fermentation is completed.
[0069] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the utility model.
Claims
1. An industrial-scale bioreactor device for static culture capable of heat transfer, comprising a tank (9), characterized in that: The tank body (9) comprises a tank top, a tank body and a tank bottom. The tank top is provided with a sight glass (1) and a manhole (8). The tank body is provided with a temperature control part, which includes an annular coil device and a jacket device. The annular coil device is sleeved in the tank body to control the central temperature of the fermentation liquid in the tank body. The jacket device is arranged around the outside of the tank body to form a jacket layer to control the temperature of the fermentation liquid near the inner wall of the tank body. The annular coil device comprises a hot / cold water circulating water outlet (3) of the annular coil, an annular coil (4) and a hot / cold water circulating water inlet (12) of the annular coil. The annular coil (4) is located at a central position inside the tank body and spirally extends to the outer side wall of the middle and upper part of the tank body to form the hot / cold water circulating water outlet (3) of the annular coil, and spirally extends to the outer side wall of the middle and lower part of the tank body to form the hot / cold water circulating water inlet (12) of the annular coil.
2. The industrial-scale bioreactor apparatus according to claim 1, wherein: The jacket device comprises a hot water / cold water circulating water inlet (5) of the jacket, a jacket (11) and a hot water / cold water circulating water outlet (10) of the jacket, wherein the hot water / cold water circulating water inlet (5) of the jacket is fixedly connected to the outer wall of the middle and lower part of the jacket (11), and the hot water / cold water circulating water outlet (10) of the jacket is fixedly connected to the outer wall of the middle and upper part of the jacket (11).
3. The industrial-scale bioreactor device according to claim 1, characterized in that The can body is provided with a ventilation pipe (2), which is sleeved inside the can body, one end of which passes through the inner wall of the can body and extends outward to the outer wall of the middle and upper part of the can body, and the other end of which passes through the inner wall of the can body and extends downward to the bottom.
4. The industrial-scale bioreactor device according to claim 2, characterized in that The can body is provided with a ventilation pipe (2), which is sleeved inside the can body, one end of which passes through the inner wall of the can body and extends outward to the outer wall of the middle and upper part of the can body, and the other end of which passes through the inner wall of the can body and extends downward to the bottom.
5. The industrial-scale bioreactor device according to claim 1, characterized in that The bottom of the tank is provided with a stirring device, which comprises a stirrer (13) and a stirring motor (14).
6. The industrial-scale bioreactor device according to claim 2, characterized in that The bottom of the tank is provided with a stirring device, which comprises a stirrer (13) and a stirring motor (14).
7. The industrial-scale bioreactor device according to claim 3, characterized in that The bottom of the tank is provided with a stirring device, which comprises a stirrer (13) and a stirring motor (14).
8. The industrial-scale bioreactor device according to claim 4, characterized in that The bottom of the tank is provided with a stirring device, which comprises a stirrer (13) and a stirring motor (14).
9. An industrial-scale bioreactor device according to any one of claims 5 to 8, characterized in that The output end of the stirring motor is rigidly connected to the rotating shaft of the stirrer, and a stirring blade is fixedly connected to the rotating shaft of the stirrer.
10. The industrial-scale bioreactor device according to any one of claims 1 to 9, characterized in that: The tank bottom is provided with a discharge port (6) and supporting legs (7).
11. The industrial-scale bioreactor device according to any one of claims 1 to 10, characterized in that: The material of the tank body (9) is stainless steel.