Optical signal collection lens and sample analysis equipment

Abstract

The embodiment of the utility model provides an optical signal collecting lens and sample analysis equipment, and relates to the field of sample analysis. The optical signal collecting lens comprises a first transmission part, a second transmission part and a collecting part which are sequentially arranged at intervals. The first transmission part is provided with a fluid channel, the fluid channel is used for conveying a sample, the sample generates a lateral light beam after receiving irradiation of the laser, and a baffling surface is formed on one side, close to the second transmission part, of the first transmission part and is used for correcting spherical aberration of the lateral light beam. In the working process of the optical signal collecting lens, a sample can be conveyed through the fluid channel, the sample generates lateral light beams while receiving laser irradiation, the lateral light beams are transmitted from the first transmission part to the second transmission part and the collecting part, the baffling surface is used for correcting spherical aberration of the light beams transmitted by the first transmission part, and the collecting part is used for collecting the light beams transmitted by the second transmission part. Therefore, the lateral light beam transmission and collection effect is improved. The sample analysis equipment comprises an optical signal collection lens and has all functions of the optical signal collection lens.

Description

Technical Field

[0001] The utility model relates to the field of sample analysis, and in particular to an optical signal collecting lens and a sample analysis device. Background Art

[0002] Flow cytometer is a common sample analysis device. It is an optical instrument that can detect the physical and chemical characteristics of individual cells, internal components and surface markers based on laser fluorescence activation and light scattering theory. It is often used in biological cell research and auxiliary diagnosis of medical diseases. In the flow cytometer, microscopic particles are in a suspended state and are transported to the light detection area in an orderly manner by its liquid flow device. After being irradiated by the laser, the particles generally emit scattered light and fluorescence in all directions.

[0003] Flow cytometers can collect scattered light in the front direction of fluid samples and scattered light and fluorescence in the side direction. In order to maximize the collection of light signals, it is usually necessary to optimize the surface shape, curvature radius, mirror material and other factors of the lens. However, when existing lenses transmit and collect lateral light beams, they are limited by the mirror shape, resulting in poor light beam propagation effects. Utility Model Content

[0004] The utility model provides an optical signal collection lens and a sample analysis device, which can optimize the transmission mirror appearance compared to the prior art, thereby improving the light beam transmission and collection effects.

[0005] The embodiments of the present invention can be implemented as follows:

[0006] The embodiment of the utility model provides an optical signal collecting lens, which comprises:

[0007] A first transmission part, a second transmission part and a collection part are arranged in sequence and at intervals;

[0008] Among them, the first transmission part is provided with a fluid channel, and the fluid channel is used to transport samples. The sample generates a lateral light beam after receiving laser irradiation. A deflection surface is formed on the side of the first transmission part close to the second transmission part, and the deflection surface is used to correct spherical aberration of the lateral light beam.

[0009] Optionally, the deflecting surface is wavy and concave.

[0010] Optionally, the first transmission portion further includes a reflective surface, and the refractive surface is a curved surface and is located on a side of the fluid channel away from the second transmission portion.

[0011] Optionally, the first transmission portion further includes a refractive surface, which is a plane and is located between the reflective surface and the fluid channel.

[0012] Optionally, the reflective surface is coated with a reflective film.

[0013] Optionally, the reflective film is a dielectric film.

[0014] Optionally, the second transmission part is a convex lens.

[0015] Optionally, the second transmission part is a triplet lens, and includes a first sub-lens, a second sub-lens and a third sub-lens which are sequentially arranged along a direction from the collecting part to the first transmission part.

[0016] Optionally, the first sub-lens is arc-shaped, and a middle position of the first sub-lens is closer to the collecting portion than to both ends;

[0017] And / or, the third sub-lens is arc-shaped, and a middle position of the third sub-lens is closer to the first transmission portion relative to both ends.

[0018] An embodiment of the utility model further provides a sample analysis device, comprising an optical signal collecting lens.

[0019] The beneficial effects of the optical signal collection lens and sample analysis device of the embodiment of the utility model include, for example:

[0020] The optical signal collection lens includes a first transmission part, a second transmission part and a collection part which are arranged in sequence at intervals; wherein the first transmission part is provided with a fluid channel, the fluid channel is used to transport samples, the samples are used to receive laser irradiation and generate lateral light beams, and a deflection surface is formed on the side of the first transmission part close to the second transmission part, and the deflection surface is used to correct spherical aberration of the lateral light beams. During operation, the optical signal collection lens can transport samples through the fluid channel, and make the samples generate lateral light beams while receiving laser irradiation, wherein the lateral light beams are transmitted from the first transmission part to the second transmission part and the collection part, and the deflection surface is used to correct spherical aberration of the light beams transmitted by the first transmission part, thereby improving the light beam transmission and collection effects.

[0021] The sample analysis device includes an optical signal collection lens, which can optimize the appearance of each transmission part so that the propagation efficiency of the lateral light beam generated after the sample is irradiated is higher, thereby collecting the lateral light beam with maximum efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 The structure of the optical signal collecting lens provided in the embodiment of the utility model is schematically shown. Figure 1 ;

[0024] Figure 2 A schematic diagram of the operation of the optical signal collecting lens provided in an embodiment of the utility model;

[0025] Figure 3 The structure of the optical signal collecting lens provided in the embodiment of the utility model is schematically shown. Figure 2 ;

[0026] Figure 4 It is a schematic structural diagram of the first transmission unit provided in an embodiment of the utility model.

[0027] Icon: 100 - optical signal collecting lens; 110 - first transmission part; 111 - fluid channel; 112 - deflecting surface; 113 - refractive surface; 114 - reflecting surface; 115 - reflecting film; 120 - second transmission part; 121 - first sub-lens; 122 - second sub-lens; 123 - third sub-lens; 130 - collecting part; 200 - laser. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0029] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0031] In the description of the present utility model, it should be noted that if the terms "upper", "lower", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the utility model product is usually placed when used. It is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present utility model.

[0032] In addition, the terms “first”, “second”, etc., if used, are merely used to distinguish between the descriptions and should not be understood as indicating or implying relative importance.

[0033] The terms "comprises," "comprising," or any other variation thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0034] Unless otherwise clearly specified and limited, the terms "disposed", "connected" and the like should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.

[0035] It should be noted that, in the absence of conflict, the features in the embodiments of the present invention may be combined with each other.

[0036] Please refer to Figure 1 and Figure 2 The optical signal collection lens 100 and the sample analysis device provided in the embodiment of the utility model can solve the above problems, which will be described in detail below.

[0037] refer to Figure 1 , Figure 2 and Figure 4 The optical signal collecting lens 100 includes a first transmission part 110, a second transmission part 120 and a collecting part 130 which are arranged in sequence;

[0038] Among them, the first transmission part 110 is provided with a fluid channel 111, the fluid channel 111 is used to transport samples, the samples are used to receive the irradiation of the laser 200 and generate a divergent light beam, and a deflection surface 112 is formed on one side of the first transmission part 110 close to the second transmission part 120, and the deflection surface 112 is used to correct spherical aberration of the divergent light beam.

[0039] During operation, the optical signal collecting lens 100 can transport samples through the fluid channel 111, and generate a divergent light beam while the sample is irradiated by the laser 200. The divergent light beam is then transmitted from the first transmission part 110 to the second transmission part 120 and the collecting part 130. The deflection surface 112 is used to correct the spherical aberration of the light beam transmitted by the first transmission part 110, thereby improving the light beam transmission and collection effects.

[0040] Specifically, the deflecting surface 112 is wavy and concave, and the wavy shape means that the surface of the deflecting surface 112 is an arc with inconsistent curvature, which can also be understood as different positions on the deflecting surface 112 have undulating changes; the concave shape means that the two ends of the deflecting surface 112 are closer to the second transmission part 120 than the middle position, and the two ends of the deflecting surface 112 refer to the two ends of the deflecting surface 112 in the extension direction of the fluid channel 111. The optical signal collection lens 100 limits the shape of the deflecting surface 112, so that the transmission of the light beam passing through the deflecting surface 112 is more efficient, which is reflected in the better collection effect on the subsequent collection part 130, thereby achieving the maximum efficiency of collecting the lateral light beam.

[0041] It is worth noting that the fluid channel 111 is linear, and the extension direction of the fluid channel 111 is perpendicular to the direction from the first transmission part 110 to the second transmission part 120 to the collection part 130. In addition, the laser 200 irradiates the sample and generates a forward beam and a side beam at the same time, and the optical signal collection lens 100 is used to collect the side beam.

[0042] In addition, in order to make the surface shape of the baffle surface 112 more accurately determined, the surface shape calculation equation of the baffle surface 112 can be defined as: z(h)=h 2 / R{1+[1-(1+k)h 2 / R 2 ] 1 / 2}+A2h 2 +A4h 4 +A6h 6 +A8h 8 +A 10 h 10 +…+A n h n By using this calculation formula to determine the specific position of each point on the baffle surface 112, the processing convenience can be improved and repeated processing is convenient.

[0043] Wherein, h is the distance between each point on the deflection surface 112 and the optical axis, R is the radius of curvature, k is the quadratic constant, A2, A4, A6, A8, A 10 …A n is the coefficient of the higher order surface.

[0044] Specifically, according to the formula h=(x 2 +y 2 ) 1 / 2 , calculate the actual distance, where x and y are the relative position coordinates of each point.

[0045] Furthermore, the specific values ​​of each parameter can be obtained by inferring the obtained optical signal effect based on multiple actual experiments. As a preferred embodiment, R = -31.851, k = 0, A2 = 0, A4 = 1.041 -3 , A6=8.762 -5 , A8=-4.93 -6 , A 10 =3.103 -7 It should be noted that when A n The larger the n value is, the smaller the specific parameter is. Therefore, when n reaches a certain value, its influence on the final calculated value of z(h) is quite limited. Under conventional calculation conditions, n=10 is sufficient, that is, the preferred surface shape calculation equation for the baffle surface 112 is: z(h)=h 2 / R{1+[1-(1+k)h 2 / R 2 ] 1 / 2}+A2h 2 +A4h 4 +A6h 6 +A8h 8 +A 10 h 10 .

[0046] refer to Figure 1-Figure 3 The first transmission portion 110 further includes a refractive surface 113 , which is a plane and is located on a side of the fluid channel 111 away from the second transmission portion 120 .

[0047] When a divergent light beam is generated, the divergent light beam first passes through the side wall of the fluid channel 111 and then is transmitted to the refractive surface 113 . After being refracted by the refractive surface 113 , the divergent light beam continues to be transmitted in a direction away from the fluid channel 111 .

[0048] refer to Figure 1-Figure 3 The first transmission part 110 further includes a reflective surface 114 , which is a curved surface and is located on a side of the refractive surface 113 away from the second transmission part 120 . That is, the refractive surface 113 is located between the reflective surface 114 and the fluid channel 111 .

[0049] During operation, the reflective surface 114 is used to receive the light beam transmitted through the refractive surface 113 and then reflect it back to the refractive surface 113 to make it move toward the direction close to the second transmission part 120 , thereby reflecting the light beam.

[0050] Specifically, in order to improve the reflection effect, the surface of the reflection surface 114 can also be coated with a reflection film 115, and the reflection film 115 can be a dielectric film or a metal film. Among them, the dielectric film can be composed of multiple layers of oxide materials with high and low refractive indices, and the overall material thickness is about 0.1nm to 10nm, and there is no limitation on its specific thickness size. After passing through the multi-layer structure, the light beam is folded back multiple times, thereby realizing the reflection of most of the light beam, thereby improving the reflectivity, so that its actual reflectivity can reach more than 99%. Although the reflectivity is also limited by the impact of the incident angle, by setting a refractive surface 113 on the side of the reflection surface 114 close to the fluid channel 111, the angle of the light beam close to the reflection surface 114 can also be refracted and adjusted to improve the reflectivity of the light beam after contacting the reflection surface 114, thereby improving the light beam propagation effect. The metal film is generally composed of metals such as gold, silver, and aluminum materials. Different from the inconsistent reflectivity of individual metal materials in different bands, in the visible light band, considering the cost and stability, silver film or aluminum film is preferred, and the reflectivity can reach more than 90%.

[0051] refer to Figure 1 and Figure 3 , the second transmission part 120 is a convex lens.

[0052] In this embodiment, the second transmission portion 120 is a triplet lens, and includes a first sub-lens 121 , a second sub-lens 122 , and a third sub-lens 123 which are sequentially arranged along a direction from the collecting portion 130 to the first transmission portion 110 .

[0053] Of course, in other embodiments of the present invention, the second transmission unit 120 can also be a single lens, a doublet lens, a quadruplet lens, a quintuplet lens, etc., and the specific number of sub-lenses therein is not limited.

[0054] refer to Figure 1-Figure 3 The first sub-lens 121 is arc-shaped, and the middle position of the first sub-lens 121 is closer to the collecting portion 130 relative to the two ends; and the third sub-lens 123 can also be arc-shaped, and the middle position of the third sub-lens 123 is closer to the first transmission portion 110 relative to the two ends; and the second sub-lens 122 is a convex lens, and the two sides of the second sub-lens 122 are glued to the first sub-lens 121 and the third sub-lens 123 respectively.

[0055] During the propagation of the light beam, after passing through the deflection surface 112, the light beam will enter the air portion between the first transmission portion 110 and the second transmission portion 120, and then reach the third sub-lens 123 and sequentially pass through the second sub-lens 122 and the first sub-lens 121 before being emitted, and then converge to the collection portion 130 to form a light spot with concentrated energy.

[0056] It is worth noting that the collecting part 130 generally places detectors, such as silicon-based photodetectors such as CCD, PD, APD or PMT, and may also place optical elements with other functions, including but not limited to apertures, pinholes or optical fiber bundles, etc., for controlling the transmitted light.

[0057] In addition, the optical signal collecting lens 100 adopts a folding structure, which adds a reflector system to the traditional refractive material. Since the chromatic aberration correction of the refractive material in a wide band is relatively difficult, even if the combination of crown glass and flint glass is continuously increased, the remaining secondary spectral chromatic aberration is relatively large, while the pure reflection system has no chromatic aberration in any desired bandwidth range, and it also promotes a simple structure and a reduction in the number of lenses. Therefore, by adding a reflector system to the traditional refractive material, the difficulty of chromatic aberration correction can be reduced, and the lens structure can be simplified.

[0058] Since the fluorescence signal excited by flow cytometry is a wide spectrum from 400-850nm, some extend to 350-900nm, or even 200-1500nm, it is very difficult to correct chromatic aberration in such a wide spectral range.

[0059] The optical signal collecting lens 100 introduces a spherical reflective surface 114, and optimizes the center of its curvature to a non-spherical deflecting surface 112 to correct spherical aberration, and the deflecting surface 112 acts as a stop, so that the system has a larger uniform image field. Since the structure of the flow liquid flow cavity is highly light-transmissive and its material is generally made of optical glass, part of the surface of the optical lens can be made into an integral structure with the liquid flow cavity, such as the first transmission part 110, so that the optical material composed of the refractive surface 113 and the reflective surface 114 can be glued together by optical gel or molecular bonding. If the fluid channel 111 is made of the same material, then the refractive surface 113 is not needed, and the reflective surface 114 and the deflecting surface 112 can be directly optically processed. Specifically, CNC grinding or other processing methods can be adopted to optimize the refractive surface 113 and the deflecting surface 112 to a reasonable surface shape. By processing the refractive surface 113 and the deflecting surface 112 of the optical lens separately from the fluid channel 111, the processing yield can be guaranteed. The reflective surface 114 may be a spherical surface, a parabolic surface or other aspherical surfaces. Considering the difficulty of processing and the sensitivity of manufacturing errors, a spherical surface is used in this embodiment, and the deflecting surface 112 is used to correct the spherical aberration introduced by the reflective surface 114. The residual aberration is the change of spherical aberration with wavelength or some high-order astigmatism and the oblique light spherical aberration introduced by off-axis light. Although the reflective surface 114 and the liquid flow cavity of the same material can reduce the chromatic aberration to a lower level, since the light first emerges from the aqueous solution to the glass and then from the glass to the air, the change of the refractive index of the material will also produce a certain chromatic aberration or secondary spectrum in the wide spectrum domain. The optical signal collection lens 100 is provided with a second transmission part 120, so that the lens of the second transmission part 120 uses flint glass and crown glass with high and low refractive indices to work together to correct the chromatic aberration or secondary spectrum, and the number and thickness of the flint glass and crown glass are not limited, and it can be a double cemented lens or a triple cemented lens.

[0060] The second transmission part 120 is a triple-cemented lens, which is composed of two pieces of high-refractive-index flint glass and one piece of low-refractive-index biconvex crown glass, and is used to correct the remaining chromatic aberration and coma astigmatism and focus the light beam. The flint glass materials of the first sub-lens 121, the second sub-lens 122 and the third sub-lens 123 can be the same material or different materials, and there is a certain degree of freedom in selection. The central low-refractive-index crown glass is generally K9 or BK7 glass, which has the characteristics of low cost and stable performance.

[0061] There is air between the first transmission part 110 and the second transmission part 120 to serve as a spacer. Since the optical system composed of the reflection and deflection surface 112 is in an infinite conjugate form, there is no restriction on the air spacing distance between the two parts. Corresponding optical elements or other devices can be inserted in the middle, which increases the freedom in optical and structural design.

[0062] It is worth noting that the effects that can be achieved by the optical signal collection lens 100 include: numerical aperture reaches 1.21, field of view reaches 500um, workpiece distance is greater than 2mm, RMS radius of the focusing light plate is less than 50um, wavelength covers 400nm~900nm, and magnification is 9 times. By limiting various parameters, an optical signal collection lens 100 with high numerical aperture, long working distance and large field of view is obtained.

[0063] It should be noted that the specific setting parameters of the optical signal collection lens 100 in this embodiment can refer to the following Table 1 (unit: mm):

[0064]

[0065] Table 1

[0066] It is worth noting that the radius, interval and refractive index parameters in Table 1 are mostly range values, and the preferred values ​​are usually middle values ​​close to the range values, but the specific selected values ​​in the design process can be kept within the allowable range.

[0067] An embodiment of the utility model also provides a sample analysis device, which can be specifically a flow cytometer and includes an optical system, a liquid flow system and a mechanical motion system, wherein a light signal collection lens 100 is applied to the optical system. During the operation of the flow cytometer, the liquid flow system can be used to allow single cells to be stably presented and pass through the position where the laser 200 is focused and irradiated one by one. When the laser 200 is irradiated on the cell surface, scattered light and excited fluorescence are formed. The light signal collection lens 100 is used to collect and analyze the light signal, and then obtain the corresponding information of the cell, specifically including cell size, surface complexity and group type.

[0068] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. An optical signal collecting lens, characterized in that: include: A first transmission part (110), a second transmission part (120) and a collection part (130) are arranged in sequence and at intervals; The first transmission part (110) is provided with a fluid channel (111), and the fluid channel (111) is used to transport a sample, and the sample generates a lateral light beam after receiving irradiation from a laser (200). A deflection surface (112) is formed on a side of the first transmission part (110) close to the second transmission part (120), and the deflection surface (112) is used to correct spherical aberration of the lateral light beam.

2. The optical signal collecting lens according to claim 1, characterized in that: The deflecting surface (112) is wave-concave.

3. The optical signal collecting lens according to claim 1, characterized in that: The first transmission part (110) further comprises a reflection surface (114), wherein the reflection surface (114) is a curved surface and is located on a side of the fluid channel (111) away from the second transmission part (120).

4. The optical signal collecting lens according to claim 3, characterized in that: The first transmission portion (110) further comprises a refractive surface (113), wherein the refractive surface (113) is a plane and is located between the reflective surface (114) and the fluid channel (111).

5. The optical signal collecting lens according to claim 3, characterized in that: The reflective surface (114) is coated with a reflective film (115).

6. The optical signal collecting lens according to claim 5, characterized in that: The reflective film (115) is a dielectric film.

7. The optical signal collecting lens according to claim 1, characterized in that: The second transmission part (120) is a convex lens.

8. The optical signal collecting lens according to claim 1, characterized in that: The second transmission part (120) is a triplet lens, and comprises a first sub-lens (121), a second sub-lens (122), and a third sub-lens (123) which are sequentially arranged along a direction from the collection part (130) to the first transmission part (110).

9. The optical signal collecting lens according to claim 8, characterized in that: The first sub-lens (121) is arc-shaped, and the middle position of the first sub-lens (121) is closer to the collecting portion (130) than the two ends; And / or, the third sub-lens (123) is arc-shaped, and the middle position of the third sub-lens (123) is closer to the first transmission part (110) relative to both ends.

10. A sample analysis device, characterized in that: It comprises the optical signal collecting lens as described in any one of claims 1 to 9.

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