Liquid drop micro-fluidic chip

Abstract

The utility model relates to a liquid drop micro-fluidic chip which comprises a shell, at least two reaction cavities and a micro-channel are arranged in the shell, every two adjacent reaction cavities are connected through the micro-channel, magnetic beads are stored in at least one reaction cavity, a guide part is arranged on the shell, the guide part is arranged on one side of the reaction cavities and the micro-channel, and the micro-channel is arranged on one side of the guide part. The guide plate is used for guiding the magnets for adsorbing the magnetic beads; and the photosensitive valve is arranged in the micro-channel and can close and open the micro-channel. The guide part can enable the magnet to move according to the preset path, so that the distance between the magnet and the magnetic beads is kept within the preset range, it is ensured that the magnet has enough adsorption force on all the magnetic beads, and then the magnetic beads are prevented from remaining in the micro-channel and the reaction cavity. The photosensitive valves enable the two adjacent reaction cavities to be communicated and closed in a controlled manner, so that the liquid drops are transferred in the plurality of reaction cavities in a controlled manner, and the control effect of the liquid drop micro-fluidic chip is ensured.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a droplet microfluidic chip. Background Art

[0002] In microfluidic manipulation technology, the driving and control of microfluidics is the premise and basis for achieving precise manipulation of trace amounts (nanoliter to picoliter) of liquid.

[0003] In the prior art, a magnet is arranged outside the shell of the microfluidic chip, and the magnet is used to drive the magnetic beads in the shell through the microchannel, so that the magnetic beads move in adjacent reaction pools. Since the magnetic beads can absorb the droplets in the reaction pool, the droplets are driven to move between the reaction pools.

[0004] However, on the one hand, the magnet is located outside the shell, and the distance between the magnet and the reaction pool (and microchannel) is difficult to control, resulting in unstable attraction of the magnet to the magnetic beads, which in turn leads to residual magnetic beads. In addition, the microchannels between two adjacent reaction pools are always connected, and a small amount of droplets in the reaction pool enter the adjacent reaction pool without the control of the magnetic beads, resulting in poor control effect of the microfluidic chip. Utility Model Content

[0005] The purpose of the utility model is to provide a droplet microfluidic chip to solve the above technical problems.

[0006] To achieve the above object, the utility model provides a droplet microfluidic chip, comprising:

[0007] A shell, wherein at least two reaction chambers and a microchannel are arranged in the shell, the two adjacent reaction chambers are connected by the microchannel, at least one of the reaction chambers stores magnetic beads, and the shell is provided with a guide portion, which is arranged on one side of the reaction chamber and the microchannel and is used to guide the magnet that absorbs the magnetic beads;

[0008] A photosensitive valve is arranged in the microchannel, and the photosensitive valve can close and open the microchannel.

[0009] Optionally, the guide portion is a guide hole or a guide groove.

[0010] Optionally, the at least two reaction chambers are spaced apart along a first direction, and the guide hole or the guide groove extends along the first direction.

[0011] Optionally, the photosensitive valve is made of a photosensitive material, and the droplet microfluidic chip further comprises a light source, which can irradiate and heat the photosensitive valve to cause thermal deformation of the photosensitive valve and open the microchannel.

[0012] Optionally, the light source includes an infrared light source, a laser light source, a fluorescent light source, or an ultraviolet light source.

[0013] Optionally, a receiving groove is provided on the shell, and the light source is arranged in the receiving groove.

[0014] Optionally, the accommodating groove is opened at the bottom, side or top of the shell.

[0015] Optionally, the number of the light sources is consistent with the number of the photosensitive valves, and are arranged one-to-one with the photosensitive valves.

[0016] Optionally, at least the housing between the light source and the photosensitive valve is made of a transparent material.

[0017] Optionally, the shell includes a base and a cover, the base and / or the cover are provided with a reaction groove and a channel groove, and the cover is covered on the base to form the reaction chamber and the microchannel.

[0018] Optionally, the base body and / or the cover body are made of ABS resin, polycarbonate, polyethylene, polypropylene, or polyethylene terephthalate plastics.

[0019] Optionally, the base body and the cover body are connected by ultrasonic welding or bonding.

[0020] Optionally, the wall of the reaction chamber is arc-shaped.

[0021] Optionally, the arc is an elliptical arc.

[0022] Optionally, an angle between a tangent line at a connection between the arc and the microchannel and the microchannel is less than 80°.

[0023] Optionally, the width of the microchannel is 5 microns to 1500 microns.

[0024] Optionally, the maximum width of the reaction chamber is 100 microns to 2000 microns.

[0025] Optionally, the maximum width of the reaction chamber is greater than the width of the microchannel.

[0026] Optionally, the ratio of the maximum width of the reaction chamber to the width of the microchannel is greater than 1:3.

[0027] Optionally, the depth of the reaction chamber and / or the microchannel is 0.01 mm-10 mm.

[0028] As can be seen from the above, the technical solution provided by the utility model is that a guide part is provided on the shell, and the guide part is provided on one side of the reaction chamber and the microchannel, and is used to guide the magnet that absorbs the magnetic beads. The guide part can make the magnet move along a preset path, so that the distance between the magnet and the magnetic beads is kept within a preset range, ensuring that the magnet has a sufficiently large adsorption force on all the magnetic beads, thereby preventing the magnetic beads from remaining in the microchannel and the reaction chamber.

[0029] The photosensitive valve enables two adjacent reaction chambers to be connected and closed in a controlled manner, thereby enabling droplets to be transferred in a plurality of reaction chambers in a controlled manner, thereby ensuring the control effect of the droplet microfluidic chip.

[0030] The photosensitive valve is made of photosensitive material. When the light source does not illuminate the photosensitive valve, the photosensitive valve is solid. After the light source illuminates the photosensitive valve, the photosensitive valve changes from solid to semi-solid or liquid, causing the microchannel interfacial tension to change. The microchannel opens, and the magnetic beads can pass through the photosensitive valve under the adsorption force of the magnet. When the light source no longer illuminates the photosensitive valve, the photosensitive valve can become solid again to block the microchannel. The photosensitive valve has good controllability, which can ensure that the droplet microfluidic chip has a good control effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a cross-sectional view of a droplet microfluidic chip provided by an embodiment of the utility model;

[0032] Figure 2 yes Figure 1 A partial enlarged view of the middle A;

[0033] Figure 3a It is an exploded view of the droplet microfluidic chip provided by the embodiment of the utility model;

[0034] Figure 3b yes Figure 3a A partial enlarged view of point B in the middle;

[0035] Figure 4 It is a structural schematic diagram of a base provided by an embodiment of the utility model;

[0036] Figure 5 It is a schematic diagram of the structure of the droplet microfluidic chip provided by the embodiment of the utility model;

[0037] Figure 6 This is an exploded view of a droplet microfluidic chip from another perspective provided by an embodiment of the utility model.

[0038] In the figure:

[0039] 1. Shell; 11. Reaction chamber; 111. Warehouse wall; 12. Microchannel; 13. Guide hole; 14. Accommodation groove; 15. Cover body; 151. First sub-hole; 16. Base body; 161. Second sub-hole; 162. Reaction groove; 163. Channel groove. DETAILED DESCRIPTION

[0040] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the utility model, rather than to limit the utility model. It is also necessary to explain that, for the convenience of description, only the parts related to the utility model are shown in the accompanying drawings, rather than all.

[0041] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "upper", "lower", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.

[0042] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0043] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0044] This embodiment provides a droplet microfluidic chip 100 to ensure stable adsorption of magnetic beads by a magnet, avoid residual magnetic beads, and improve the control effect of the droplet microfluidic chip.

[0045] like Figure 1 and Figure 2As shown, the droplet microfluidic chip 100 provided in this embodiment includes a housing 1 , in which at least two reaction chambers 11 and a microchannel 12 are arranged, and two adjacent reaction chambers 11 are connected through the microchannel 12 .

[0046] like Figure 2-Figure 4 As shown, in order to facilitate the formation of the reaction chamber 11 and the microchannel 12, the shell 1 includes a base 16 and a cover 15, the base 16 is provided with a reaction groove 162 and a channel groove 163, and the cover 15 is covered on the base 16, so that the reaction groove 162 and the channel groove 163 can be closed to form the reaction chamber 11 and the microchannel 12.

[0047] Of course, in other optional embodiments, the reaction groove 162 and the channel groove 163 may be opened on the cover body 15, or the reaction groove 162 and the channel groove 163 may be opened on both the cover body 15 and the base body 16, and the cover body 15 and the base body 16 are covered to form the reaction chamber 11 and the microchannel 12.

[0048] The reaction groove 162 and the channel groove 163 can be formed by machining, photolithography or pressure welding, and then the reaction groove 162 and the channel groove 163 are closed by connecting the substrate 16 and the cover 15, so as to form the reaction chamber 11 and the microchannel 12. The formation method of the reaction chamber 11 and the microchannel 12 is simple and the processing cost is low. Optionally, in this embodiment, the substrate 16 and the cover 15 are connected by ultrasonic welding or bonding, such as bonding the substrate 16 and the cover 15 by double-sided tape, so as to connect the two.

[0049] Optionally, in the present embodiment, a plurality of reaction grooves 162 are arranged in sequence along a direction, that is, at least two reaction chambers 11 are arranged in sequence along a direction. For ease of explanation, in the present embodiment, it is stipulated that the direction in which the at least two reaction chambers 11 are arranged in sequence is a first direction L, the width direction of the microchannel 12 and the width direction of the reaction chamber 11 are a second direction W, the direction in which the cover body 15 and the base body 16 are arranged is a third direction H, the depth direction of the microchannel 12 and the depth direction of the reaction chamber 11 are consistent with the third direction H, and the third direction H, the first direction L and the second direction W are perpendicular to each other.

[0050] When the droplet microfluidic chip 100 is used, the third direction H can be consistent with the vertical direction, and the first direction L and the second direction W are horizontal directions. Of course, when the droplet microfluidic chip 100 is used, the droplet microfluidic chip 100 can be placed at an angle, in which case the third direction H is set at an acute angle to the vertical direction, the first direction L is set at an acute angle to the horizontal direction, and the second direction W is consistent with the horizontal direction. It can be understood that when the droplet microfluidic chip 100 is used, the actual directions of the first direction L, the second direction W and the third direction H are not limited thereto, and they are determined according to the actual placement direction of the droplet microfluidic chip 100.

[0051] In order to transfer the droplets in the reaction chamber 11, at least one reaction chamber 11 stores magnetic beads, and a magnet can be arranged outside the reaction chamber 11 and the microchannel 12. The magnet can absorb the magnetic beads, and the magnetic beads are transferred by the magnet, thereby realizing the transfer of the droplets. The magnet is located outside the reaction chamber 11 and the microchannel 12, so that it is easy to manipulate the magnet.

[0052] In this embodiment, in order to keep the magnet and the magnetic beads within a preset distance range, thereby ensuring that the magnet has a sufficiently large adsorption force on all the magnetic beads, thereby preventing the magnetic beads from remaining in the microchannel 12 and the reaction chamber 11, a guide portion is provided on the housing 1, and the guide portion is provided on one side of the reaction chamber 11 and the microchannel 12, and is used to guide the magnet that adsorbs the magnetic beads. The guide portion can make the magnet move along a preset path, thereby keeping the distance between the magnet and the magnetic beads within a preset range.

[0053] like Figure 4 and Figure 5 As shown, in a specific embodiment, the guide portion is a guide hole 13, and an external device controls the magnet to leave from above or below the guide hole 13. When it is necessary to drag the magnetic beads from one reaction chamber 11 to the next reaction chamber 11, the magnet moves to the guide hole 13 and moves along the guide hole 13 to drag the magnetic beads. Specifically, the guide hole 13 is located on one side of the microchannel 12 along the second direction W. The guide hole 13 can shorten the distance between the magnet and the magnetic beads, increase the adsorption force between the magnet and the magnetic beads, and make the magnet adsorb the magnetic beads more stably to avoid residual magnetic beads.

[0054] The guide hole 13 can extend along the first direction L, that is, the guide hole 13 can be a long hole extending along the first direction L, so that the magnet can be arranged opposite to each microchannel 12 and the reaction chamber 11 in the second direction W in sequence, so that the magnet drags the magnetic beads into each reaction chamber 11, and at the same time, prevents the magnet from moving in the second direction W and deviating from the movement direction, resulting in residual magnetic beads. It can be understood that the guide hole 13 passes through the housing 1 along the third direction H, so that the magnet enters and exits the guide hole 13 from above or below the guide hole 13.

[0055] like Figure 5 and Figure 6 As shown, illustratively, a first sub-hole 151 is formed on the cover body 15 , a second sub-hole 161 is formed on the base body 16 , the cover body 15 is connected to the base body 16 , and the first sub-hole 151 and the second sub-hole 161 are arranged opposite to each other, thereby forming the guide hole 13 .

[0056] Of course, in other optional embodiments, the guide portion may also be a guide groove, and the guide groove may be provided on the base 16. Exemplarily, the guide groove also extends along the first direction L, and the guide groove is optionally located on one side of the microchannel 12 along the second direction W. The structure of the guide portion is not limited thereto, as long as it can limit the magnet so that the magnet moves along a preset path.

[0057] like Figure 3b and Figure 4 As shown, in order to further prevent the magnetic beads from remaining in the reactor, optionally, the chamber wall 111 of the reaction chamber 11 is arc-shaped, and the arc-shaped chamber wall 111 can reduce the tension between the droplets and the magnetic beads and the chamber wall 111, thereby preventing the droplets or magnetic beads from remaining. In this embodiment, the arc protrudes outwards to the side away from the guide hole 13.

[0058] Furthermore, the arc is an elliptical arc, thereby further avoiding residual magnetic beads or droplets.

[0059] Optionally, the angle α between the tangent line at the connection between the arc and the microchannel 12 and the microchannel 12 is less than 80°. More preferably, the angle α is less than 60°.

[0060] The droplet microfluidic chip 100 provided in this embodiment may further include a photosensitive valve (not shown in the figure), which is disposed in the microchannel 12 and can close and open the microchannel 12. The photosensitive valve enables two adjacent reaction chambers 11 to be connected and closed in a controlled manner, thereby enabling droplets to be transferred in a plurality of reaction chambers 11 in a controlled manner, thereby ensuring the control effect of the droplet microfluidic chip 100.

[0061] The photosensitive valve is made of a photosensitive material, which can be a thermally deformable material. The droplet microfluidic chip 100 also includes a light source (not shown in the figure), which can illuminate and heat the photosensitive valve to thermally deform the photosensitive valve and open the microchannel 12. Of course, in other optional embodiments, the droplet microfluidic chip 100 may not include a light source, but illuminate the photosensitive valve through an external light source.

[0062] The photosensitive material may include, but is not limited to, a mixture of iron oxide nanoparticles and paraffin, poly-N-isopropylacrylamide, a mixture of poly-N-isopropylacrylamide and paraffin, poloxamer, various glues, etc. Since the photosensitive material is a prior art and is not the inventive point of the present utility model, the specific components thereof will not be described in detail in this embodiment.

[0063] The light source may include an infrared light source, a laser light source, a fluorescent light source, and an ultraviolet light source. When the light source is irradiated, the photosensitive material absorbs the heat of the light source and undergoes a phase change. For example, when the light source does not irradiate the photosensitive valve, the photosensitive valve is solid. After the light source irradiates the photosensitive valve, the photosensitive valve changes from solid to semi-solid or liquid, causing the interfacial tension of the microchannel 12 to change. The microchannel 12 opens, and the magnetic beads can pass through the photosensitive valve under the adsorption force of the magnet. When the light source no longer irradiates the photosensitive valve, the photosensitive valve can become solid again to block the microchannel 12.

[0064] like Figure 5 and Figure 6 As shown, optionally, a receiving groove 14 is provided on the housing 1, and the light source is arranged in the receiving groove 14. On the one hand, the receiving groove 14 can shorten the distance between the light source and the photosensitive valve, thereby making it easier to heat the photosensitive valve; on the other hand, the receiving groove 14 can prevent the light source from protruding from the housing 1, thereby preventing the light source from interfering with external equipment.

[0065] The receiving groove 14 is provided at the bottom of the housing 1, and the bottom has a large operating space, so that it is convenient to process the receiving groove 14 and to install the light source. Figure 6 As shown, further, the receiving groove 14 is opened on the base 16 and is located on the side of the base 16 away from the cover 15, that is, the receiving groove 14 is opened on the lower side of the microchannel 12 so that the light source and the photosensitive valve are arranged opposite to each other.

[0066] refer to Figure 3a It is understandable that in other optional embodiments, the receiving groove 14 can also be opened on the upper part of the housing 1, such as the upper surface of the cover 15, that is, the receiving groove 14 is located above the photosensitive valve. Or the receiving groove 14 is opened on the side of the housing 1, such as the receiving groove 14 is located on one side of the photosensitive valve along the second direction W. For example, the receiving groove 14 is located on one side of the photosensitive valve along the second direction W, and the guide hole 13 is located on the other side of the photosensitive valve along the second direction W.

[0067] Continue to refer Figure 5 and Figure 6 Optionally, the number of light sources is consistent with the number of photosensitive valves, and the light sources are arranged one by one with the photosensitive valves, so that the light sources can be controlled to open and close the photosensitive valves corresponding to the light sources. Of course, in other optional embodiments, the light sources can be moved so that the light sources are directly opposite to the photosensitive valves to be opened, thereby opening the required photosensitive valves. However, when the light sources are arranged one by one with the photosensitive valves, it is easier to operate.

[0068] Exemplarily, the base 16 and / or the cover 15 are made of ABS resin, polycarbonate (PC), polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET). Preferably, at least the housing 1 between the light source and the photosensitive valve is made of a transparent material so that the light source illuminates the photosensitive valve.

[0069] like Figure 1 and Figure 3a As shown, illustratively, six reaction chambers 11 are provided in this embodiment, and the six reaction chambers 11 store magnetic bead liquid (magnetic bead liquid contains magnetic beads), labeled enzyme liquid, first washing liquid, second washing liquid, third washing liquid and substrate liquid in sequence, and each two adjacent reaction chambers 11 are connected through microchannels 12. When processing the droplet microfluidic chip 100, the photosensitive material for making the photosensitive valve is dotted in the channel groove 163 of the substrate 16, and the magnetic bead liquid, labeled enzyme liquid, first washing liquid, second washing liquid, third washing liquid and substrate liquid are filled into the reaction groove 162 respectively, and finally packaged with the cover body 15 to form a finished product.

[0070] When using a microfluidic chip, add the sample to the magnetic bead liquid. When the magnetic beads react with the sample to form a magnetic bead-antigen immune conjugate, the light source irradiates the photosensitive valve, the photosensitive valve opens the microchannel 12, and the magnet moves, which can drag the magnetic beads through the microchannel 12 and enter the reaction chamber 11 storing the labeled enzyme liquid. At this time, the magnet is taken out of the guide hole 13, and the light source no longer irradiates the previously opened photosensitive valve, so that the photosensitive valve closes the microchannel 12 again. After the magnetic bead-antigen immune conjugate reacts with the labeled enzyme liquid to form a magnetic bead-antigen-enzyme-labeled antibody conjugate, the light source irradiates the next photosensitive valve, the photosensitive valve opens the microchannel 12, and the magnet is inserted into the guide hole 13. The magnet continues to move to drag the magnetic beads into the first washing liquid, repeat the above operation, the magnetic beads enter the second washing liquid and the third washing liquid hole in turn for washing, and finally the magnetic bead-antigen-enzyme-labeled antibody conjugate enters the last reaction chamber 11 containing the substrate liquid, so that the magnetic bead-antigen-enzyme-labeled antibody conjugate reacts with the substrate to form the liquid to be detected, and the instrument collects the signal of the liquid to be detected to complete the detection.

[0071] Optionally, the width of the microchannel 12 (i.e., the dimension of the microchannel 12 along the second direction W) is 5 micrometers to 1500 micrometers, which can realize the function of fixing the photosensitive valve on the one hand, and prevent the cross-hole of droplets between different reaction chambers 11 on the other hand. The maximum width of the reaction chamber 11 (i.e., the maximum dimension of the reaction chamber 11 along the second direction W) is 100 micrometers to 2000 micrometers, so as to store sufficient droplets and realize microfluidics in cooperation with the microchannel 12.

[0072] The maximum width of the reaction chamber 11 is greater than the width of the microchannel 12. Furthermore, the maximum width of the reaction chamber 11 is much greater than the width of the microchannel 12, so that the microchannel 12 and the reaction chamber 11 cooperate to achieve microfluidics. Specifically, the ratio of the maximum width of the reaction chamber 11 to the width of the microchannel 12 is greater than 1:3, such as a ratio of 1:5, 1:10 or 1:50.

[0073] Optionally, the depth of the reaction chamber 11 and / or the microchannel 12 is 0.01 mm-10 mm.

[0074] The droplet microfluidic chip provided by the utility model can controllably realize the separation and mixing of droplets, and the droplet microfluidic chip can also be combined with other products, thereby providing assistance for the development and use of rapid diagnosis products.

[0075] Although the utility model has been described in detail above by means of general description, specific implementation methods and tests, it is obvious to those skilled in the art that some modifications or improvements can be made to the utility model. Therefore, these modifications or improvements made without departing from the spirit of the utility model are within the scope of protection claimed by the utility model.

Claims

1. A droplet microfluidic chip, characterized in that: include: A shell (1), wherein at least two reaction chambers (11) and a microchannel (12) are arranged in the shell (1), the two adjacent reaction chambers (11) are connected via the microchannel (12), at least one of the reaction chambers (11) stores magnetic beads, and the shell (1) is provided with a guide portion, the guide portion is arranged on one side of the reaction chamber (11) and the microchannel (12), and is used to guide a magnet that absorbs the magnetic beads; A photosensitive valve is arranged in the microchannel (12), and the photosensitive valve can close and open the microchannel (12).

2. The droplet microfluidic chip according to claim 1, characterized in that: The guide portion is a guide hole (13) or a guide groove.

3. The droplet microfluidic chip according to claim 2, characterized in that: The at least two reaction chambers (11) are arranged at intervals along a first direction (L), and the guide hole (13) or the guide groove extends along the first direction (L).

4. The droplet microfluidic chip according to claim 1, characterized in that: The photosensitive valve is made of a photosensitive material, and the droplet microfluidic chip further comprises a light source, which can irradiate and heat the photosensitive valve to cause thermal deformation of the photosensitive valve and open the microchannel (12).

5. The droplet microfluidic chip according to claim 4, characterized in that: The light source includes an infrared light source, a laser light source, a fluorescent light source, and an ultraviolet light source.

6. The droplet microfluidic chip according to claim 4, characterized in that: The housing (1) is provided with a receiving groove (14), and the light source is arranged in the receiving groove (14).

7. The droplet microfluidic chip according to claim 6, characterized in that: The accommodating groove (14) is opened at the bottom, side or top of the shell (1).

8. The droplet microfluidic chip according to any one of claims 4 to 7, characterized in that: The number of the light sources is consistent with the number of the photosensitive valves, and they are arranged one by one with respect to the photosensitive valves.

9. The droplet microfluidic chip according to any one of claims 4 to 7, characterized in that: At least the housing (1) between the light source and the light-sensitive valve is made of a transparent material.

10. The droplet microfluidic chip according to claim 1, characterized in that: The shell (1) comprises a base (16) and a cover (15); a reaction groove (162) and a channel groove (163) are provided on the base (16) and / or the cover (15); and the cover (15) is covered on the base (16) to form the reaction chamber (11) and the microchannel (12).

11. The droplet microfluidic chip according to claim 10, characterized in that: The base body (16) and the cover body (15) are connected by ultrasonic welding or bonding.

12. The droplet microfluidic chip according to claim 1, characterized in that: The chamber wall (111) of the reaction chamber (11) is arc-shaped.

13. The droplet microfluidic chip according to claim 12, characterized in that: The arc is an elliptical arc.

14. The droplet microfluidic chip according to claim 12 or 13, characterized in that: The angle between the tangent line at the connection between the arc and the microchannel (12) and the microchannel (12) is less than 80°.

15. The droplet microfluidic chip according to claim 1, characterized in that: The width of the microchannel (12) is 5 micrometers to 1500 micrometers.

16. The droplet microfluidic chip according to claim 1, characterized in that: The maximum width of the reaction chamber (11) is 100 micrometers to 2000 micrometers.

17. The droplet microfluidic chip according to any one of claims 1, 15 or 16, characterized in that: The maximum width of the reaction chamber (11) is greater than the width of the microchannel (12).

18. The droplet microfluidic chip according to claim 17, characterized in that: The ratio of the maximum width of the reaction chamber (11) to the width of the microchannel (12) is greater than 1:

3.

19. The droplet microfluidic chip according to any one of claims 1-7, 10, 12-13, 15-16, characterized in that: The depth of the reaction chamber (11) and / or the microchannel (12) is 0.01 mm-10 mm.

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