Extraction and purification instrument for DNA (Deoxyribose Nucleic Acid)

Abstract

The utility model relates to the technical field of purifiers, and discloses an extraction and purification instrument for DNA nucleic acid, which comprises a purifier, one end of the purifier is rotatably connected with a cabinet door, the inner side of the upper end of the purifier is fixedly connected with an ultraviolet lamp, a clamping component is inserted into the inner side of the middle end of the purifier, and two ends of the clamping component are fixedly connected with sliding components. One end of the clamping assembly is fixedly connected with a transmission assembly, during use, a sample tube is firstly inserted into the clamping assembly, then the transmission assembly drives the adjusting assembly to operate, the adjusting assembly operates to drive the extrusion assembly to move, and the extrusion assembly is matched with the positioning assembly to clamp and fix the sample tube; according to the present invention, the clamping assembly is clamped in the moving assembly, and the clamping assembly and the like are pushed into the purifier through the moving assembly so as to perform purification treatment, such that the purification instrument can rapidly fix the multiple groups of the sample tubes while performing purification treatment on the DNA nucleic acid in the sample tubes so as to reduce the purification effect reduction caused by shaking.

Description

Technical Field

[0001] The utility model relates to the technical field, and in particular to an instrument for extracting and purifying DNA nucleic acid. Background Art

[0002] The DNA nucleic acid extraction and purification instrument is a device used for purifying DNA nucleic acid.

[0003] After searching, it is proposed in Chinese application number CN202022773963.9 that a DNA / RNA nucleic acid extraction and purification instrument is provided, comprising a box shell, a turntable is arranged inside the box shell, and a placement seat is fixedly connected to the upper surface of the turntable; a placement rack is fixedly connected to the left inner wall of the box shell, and five test tube slots are evenly arranged inside the placement rack, a fixed plate is fixedly connected to the inside of the box shell, and an ultraviolet sterilization device is fixedly connected to the lower surface of the fixed plate, and the power input end of the ultraviolet sterilization device is electrically connected to an ultraviolet switch; a cooling device is arranged on the right side of the ultraviolet sterilization device, and the cooling device is fixedly connected to the lower surface of the fixed plate, and the power input end of the cooling device is electrically connected to the cooling switch. The above-mentioned prior art uses ultraviolet sterilization to disinfect the inside of the purifier, and uses the cooling device to perform cooling treatment at the same time, and uses the card slot to fix the sample tube, thereby realizing rapid positioning of the sample tube and improving the purification efficiency.

[0004] In the above-mentioned prior art, multiple groups of sample tubes are positioned by inserting the sample tubes into multiple groups of slots. Although this positioning method is convenient, DNA nucleic acid needs to be purified in a stable environment. Environmental differences and shaking of the sample tubes can easily cause the liquid inside the sample tubes to shake violently, which in turn causes the solution to become turbid during the purification process, thereby affecting the purification effect. Utility Model Content

[0005] The utility model aims to provide a DNA nucleic acid extraction and purification instrument to solve the problem that the DNA nucleic acid extraction and purification instrument in the prior art is inconvenient to quickly fix the sample tube, which makes the sample tube easy to shake, thereby making the purified solution turbid and reducing the purification effect.

[0006] The utility model provides the following technical scheme: a DNA nucleic acid extraction and purification instrument, comprising a purifier, one end of the purifier is rotatably connected to a cabinet door, and an ultraviolet lamp is fixedly connected to the inner side of the upper end of the purifier, a card connection component is inserted into the inner side of the middle end of the purifier, and sliding components are fixedly connected to both ends of the card connection component, one end of the card connection component is fixedly connected to a transmission component, and one end of the transmission component is fixedly connected to an adjustment component, the upper end of the adjustment component is fixedly connected to an extrusion component, and a positioning component is arranged on one side of the extrusion component, and the lower end of the positioning component is fixedly connected to the card connection component.

[0007] As a preferred embodiment of the above technical solution, the card-connecting assembly includes a support plate inserted inside the middle end of the purifier, and a card plate is fixedly connected to the inner side of both ends of the support plate, an insert plate is sleeved on the outer side of the card plate, and a plurality of slots are opened in the middle end of the insert plate.

[0008] As a preferred embodiment of the above technical solution, the sliding assembly includes sliding blocks fixedly connected to the outer sides of both ends of the support plate, and a sliding rail is sleeved on the outer side of one end of the sliding block away from the support plate, and the end of the sliding rail away from the sliding block is fixedly connected to the inner side of the purifier.

[0009] As a preferred embodiment of the above technical solution, the transmission assembly includes a twisting plate arranged on the outside of one end of the plug plate, and the end of the twisting plate inserted in the plug plate is fixedly connected to the first gear, the end of the first gear away from the twisting plate is rotatably connected in the plug plate, and second gears are meshed on the outside of both ends of the first gear.

[0010] As a preferred embodiment of the above technical solution, the adjustment component includes a threaded rod fixedly connected to the outer side of the middle end of the second gear, and one end of the threaded rod away from the second gear is rotatably connected to the plug plate, and a threaded block is engaged with the outer side of the middle end of the threaded rod.

[0011] As a preferred embodiment of the above technical solution, the extrusion assembly includes a push plate fixedly connected to the upper end of the threaded block, and a first clamping ring is fixedly connected to the outer side of the upper end of the push plate, and a first rubber pad is fixedly connected to the end of the first clamping ring away from the push plate.

[0012] As a preferred embodiment of the above technical solution, the positioning assembly includes a fixed block fixedly connected to the outer side of the upper end of the plug plate, and a second clamping ring is fixedly connected to the outer side of the upper end of the fixed block, and a second rubber pad is fixedly connected to one end of the second clamping ring away from the fixed block.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] When using the DNA nucleic acid extraction and purification instrument, the sample tube is first inserted into the clamping component, and then the adjustment component is driven to operate by the transmission component. After the adjustment component operates, it drives the extrusion component to move, and the sample tube is clamped and fixed by the extrusion component in cooperation with the positioning component. Subsequently, the clamping component is clamped into the moving component, and the clamping component is pushed into the purifier through the moving component for purification. When the purifier is purifying the DNA nucleic acid in the sample tube, multiple groups of sample tubes can be quickly fixed, thereby reducing the reduction in purification effect caused by shaking. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of a DNA nucleic acid extraction and purification instrument;

[0016] Figure 2 It is a schematic diagram of a cross-sectional structure of a DNA nucleic acid extraction and purification instrument from a top view;

[0017] Figure 3 It is a schematic diagram of a partial cross-sectional structure enlargement of a transmission component of a DNA nucleic acid extraction and purification instrument;

[0018] Figure 4 It is a schematic diagram of the cross-sectional structure of the upper end of a purifier of a DNA nucleic acid extraction and purification instrument;

[0019] Figure 5 for Figure 4 A schematic diagram of the enlarged structure in the middle.

[0020] In the figure: 1, purifier; 11, cabinet door; 12, ultraviolet lamp; 2, support plate; 21, card plate; 22, plug plate; 23, slot; 3, slider; 31, slide rail; 4, screw plate; 41, first gear; 42, second gear; 5, threaded rod; 51, threaded block; 6, push plate; 61, first clamping ring; 62, first rubber pad; 7, fixing block; 71, second clamping ring; 72, second rubber pad. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0022] like Figure 1 - Figure 5 As shown, the utility model provides a technical solution: a DNA nucleic acid extraction and purification instrument, comprising a purifier 1, one end of the purifier 1 is rotatably connected to a cabinet door 11, and an ultraviolet lamp 12 is fixedly connected to the inner side of the upper end of the purifier 1, a clamping component is inserted into the inner side of the middle end of the purifier 1, and sliding components are fixedly connected to both ends of the clamping component, one end of the clamping component is fixedly connected to a transmission component, and one end of the transmission component is fixedly connected to an adjustment component, the upper end of the adjustment component is fixedly connected to an extrusion component, and a positioning component is arranged on one side of the extrusion component, and the lower end of the positioning component is fixedly connected to the clamping component. When in use, the sample tube is first inserted into the clamping component, and then the adjustment component is driven to operate by the transmission component, and the extrusion component is driven to move after the operation of the adjustment component, and the sample tube is clamped and fixed by the extrusion component and the positioning component, and then the clamping component is clamped in the moving component, and the clamping component and the like are pushed into the purifier 1 through the moving component for purification treatment, so that when the purifier purifies the DNA nucleic acid in the sample tube, multiple groups of sample tubes can be quickly fixed, thereby reducing the reduction of purification effect caused by shaking.

[0023] like Figure 3 and Figure 4As shown, the snap-on assembly includes a support plate 2 inserted on the inner side of the middle end of the purifier 1, and a snap-on plate 21 is fixedly connected to the inner sides of both ends of the support plate 2, an insert plate 22 is sleeved on the outer side of the card plate 21, and a plurality of slots 23 are provided at the middle end of the insert plate 22. The sample tubes are inserted into the slots 23 in sequence for predetermined positioning, and then the insert plate 22 filled with sample tubes is sleeved on the outer side of the card plate 21 and snap-connected with the support plate 2, so as to facilitate the pre-positioning and placement of the sample tubes.

[0024] like Figure 2 As shown, the sliding assembly includes sliding blocks 3 fixedly connected to the outer sides of both ends of the support plate 2, and a sliding rail 31 is sleeved on the outer side of the end of the sliding block 3 away from the support plate 2, and the end of the sliding rail 31 away from the sliding block 3 is fixedly connected to the inner side of the purifier 1. Pushing the support plate 2 drives the sliding block 3 to slide in the sliding rail 31, thereby pushing the plug plate 22 into the purifier 1, making it easy to push the plug plate 22 into the purifier 1.

[0025] like Figure 3 As shown, the transmission assembly includes a screwing plate 4 arranged on the outer side of one end of the plug plate 22, and one end of the screwing plate 4 inserted in the plug plate 22 is fixedly connected to the first gear 41, and the end of the first gear 41 away from the screwing plate 4 is rotatably connected in the plug plate 22, and the second gears 42 are meshed on the outer sides of both ends of the first gear 41. When the screwing plate 4 is screwed, the screwing plate 4 rotates and drives the first gear 41 to rotate. After the first gear 41 rotates, it drives the second gear 42 to rotate through the meshing with the second gear 42. After the second gear 42 rotates, it synchronously drives the adjustment assembly to operate, which is convenient for driving the adjustment assembly to operate.

[0026] like Figure 3 As shown, the adjustment assembly includes a threaded rod 5 fixedly connected to the outer side of the middle end of the second gear 42, and the end of the threaded rod 5 away from the second gear 42 is rotatably connected to the plug plate 22, and a threaded block 51 is meshed on the outer side of the middle end of the threaded rod 5. After the second gear 42 rotates, the threaded rod 5 is synchronously driven to rotate. The threaded rod 5 is meshed with the threaded block 51, so that the threaded block 51 drives the extrusion assembly to move, which is convenient for driving the extrusion assembly to move, thereby clamping the sample tube.

[0027] like Figure 3 and Figure 5 As shown, the extrusion assembly includes a push plate 6 fixedly connected to the upper end of the threaded block 51, and a first clamping ring 61 is fixedly connected to the outer side of the upper end of the push plate 6, and a first rubber pad 62 is fixedly connected to the end of the first clamping ring 61 away from the push plate 6. The movement of the push plate 6 drives the first clamping ring 61 and the first rubber pad 62 to cooperate with the positioning assembly to clamp and limit the sample tube, thereby facilitating the clamping and limiting of the sample tube.

[0028] like Figure 5As shown, the positioning assembly includes a fixed block 7 fixedly connected to the outer side of the upper end of the plug plate 22, and a second clamping ring 71 is fixedly connected to the outer side of the upper end of the fixed block 7, and a second rubber pad 72 is fixedly connected to the end of the second clamping ring 71 away from the fixed block 7. The push plate 6 moves to drive the first clamping ring 61 and the first rubber pad 62 to cooperate with the second clamping ring 71 and the second rubber pad 72 to clamp and limit the sample tube, so as to facilitate the clamping of the sample tube by the first clamping ring 61 and the first rubber pad 62.

[0029] Working principle: When using the purifier to purify the nucleic acid solution in the sample tube, the sample tubes are first inserted into the slots 23 in sequence for predetermined positions, and then the plug plate 22 filled with sample tubes is sleeved on the outside of the card plate 21 and engaged with the support plate 2, and then the screw plate 4 is screwed. After the screw plate 4 rotates, it drives the first gear 41 to rotate. After the first gear 41 rotates, it drives the second gear 42 to rotate through engagement with the second gear 42. After the second gear 42 rotates, it synchronously drives the threaded rod 5 to rotate. The threaded rod 5 engages with the threaded block 51, allowing the threaded block 51 to drive the push plate 6 to move. The movement of the push plate 6 drives the first clamping ring 61 and the first rubber pad 62 to cooperate with the second clamping ring 71 and the second rubber pad 72 to clamp and limit the sample tube, and then the support plate 2 is pushed to drive the slider 3 to slide in the slide rail 31, thereby pushing the plug plate 22 into the purifier 1, and the cabinet door 11 is closed to purify.

[0030] The above embodiments are only used to illustrate the technical solution of the present invention, but not to limit it.

Claims

1. A DNA nucleic acid extraction and purification apparatus, comprising a purifier (1), characterized in that: One end of the purifier (1) is rotatably connected to a cabinet door (11), and an ultraviolet lamp (12) is fixedly connected to the inner side of the upper end of the purifier (1); a clamping assembly is inserted into the inner side of the middle end of the purifier (1), and sliding assemblies are fixedly connected to both ends of the clamping assembly; one end of the clamping assembly is fixedly connected to a transmission assembly, and one end of the transmission assembly is fixedly connected to an adjustment assembly; the upper end of the adjustment assembly is fixedly connected to an extrusion assembly, and a positioning assembly is provided on one side of the extrusion assembly, and the lower end of the positioning assembly is fixedly connected to the clamping assembly.

2. A DNA nucleic acid extraction and purification instrument according to claim 1, characterized in that: The clamping assembly comprises a support plate (2) inserted into the inner side of the middle end of the purifier (1), and a clamping plate (21) is fixedly connected to the inner side of both ends of the support plate (2), an inserting plate (22) is sleeved on the outer side of the clamping plate (21), and a plurality of slots (23) are provided at the middle end of the inserting plate (22).

3. A DNA nucleic acid extraction and purification instrument according to claim 2, characterized in that: The sliding assembly comprises sliding blocks (3) fixedly connected to the outer sides of both ends of the support plate (2), and a sliding rail (31) is sleeved on the outer side of one end of the sliding block (3) away from the support plate (2), and one end of the sliding rail (31) away from the sliding block (3) is fixedly connected to the inner side of the purifier (1).

4. A DNA nucleic acid extraction and purification instrument according to claim 2, characterized in that: The transmission assembly comprises a twisting plate (4) arranged outside one end of the plugging plate (22), and one end of the twisting plate (4) inserted in the plugging plate (22) is fixedly connected to a first gear (41), one end of the first gear (41) away from the twisting plate (4) is rotatably connected in the plugging plate (22), and second gears (42) are meshed on the outside of both ends of the first gear (41).

5. A DNA nucleic acid extraction and purification instrument according to claim 4, characterized in that: The adjustment assembly comprises a threaded rod (5) fixedly connected to the outer side of the middle end of the second gear (42), and one end of the threaded rod (5) away from the second gear (42) is rotatably connected to the plug plate (22), and a threaded block (51) is meshed with the outer side of the middle end of the threaded rod (5).

6. A DNA nucleic acid extraction and purification instrument according to claim 5, characterized in that: The extrusion assembly comprises a push plate (6) fixedly connected to the upper end of the threaded block (51), a first clamping ring (61) fixedly connected to the outer side of the upper end of the push plate (6), and a first rubber pad (62) fixedly connected to one end of the first clamping ring (61) away from the push plate (6).

7. A DNA nucleic acid extraction and purification instrument according to claim 2, characterized in that: The positioning assembly comprises a fixing block (7) fixedly connected to the outer side of the upper end of the plug plate (22), a second clamping ring (71) fixedly connected to the outer side of the upper end of the fixing block (7), and a second rubber pad (72) fixedly connected to one end of the second clamping ring (71) away from the fixing block (7).

Images