Device for preparing facial mask by using mesenchymal stem cell exosome

Abstract

The utility model provides a device for preparing a mask from mesenchymal stem cell exosomes, and belongs to the technical field of biomedicine. The device comprises an adsorption main body, a plurality of groove positions arranged in the adsorption main body, and a liquid inlet pipe and a liquid outlet pipe which are symmetrically arranged on two sides of the adsorption main body, the liquid inlet pipe and the liquid outlet pipe are communicated with each groove position, and the liquid inlet pipe is respectively connected with a supernatant inlet pipe and a normal saline inlet pipe. According to the device, the exosome supernate can uniformly flow through the mask cloth, and the exosome in the exosome supernate can be efficiently captured. Meanwhile, the device can capture the exosome under the sterile condition, and the freeze-dried facial mask is simple, rapid and efficient to prepare. In addition, the device is easy and convenient to operate and small in occupied area, and other objects do not need to be prepared.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedicine and relates to a device for preparing a facial mask by utilizing mesenchymal stem cell exosomes. Background Art

[0002] Exosomes are microscopic plasma membrane vesicle structures with a diameter of about 30-120 nm that are actively synthesized by the endoplasmic reticulum and Golgi complex and secreted into the extracellular environment. They are an important paracrine form in addition to cell-soluble cytokines. Exosomes can be secreted by a variety of cells and body fluids in the human body, such as mesenchymal stem cells (MSCs), endothelial cells, and immune cells. Exosomes have attracted the attention of researchers as a new cell-free treatment option because of their good biological activity and intercellular communication functions, and their efficacy similar to that of MSCs.

[0003] Exosomes secreted by MSCs have many advantages in beauty, such as: (1) Exosomes are taken up into cells through endocytosis, which can ensure that some biomacromolecules in the exosomes are blocked by cell membrane channels and not absorbed, and have the advantages of high biological activity and high efficiency; (2) Exosomes can be stored stably for a long time in -80 ℃ and -196 ℃ environments, can provide continuous protection during the freezing of cells, and are not easily destroyed; (3) The skin damage repair effect is good. Exosomes can inhibit the expression of the target gene α-SMA and reduce collagen I deposition, and can inhibit the differentiation of myofibroblasts during skin wound healing, thereby reducing scar formation and promoting skin wound healing; (4) Generally well tolerated, due to their small size, they avoid complications such as strong immune rejection. Exosomes derived from MSCs have been proven to have a clear protective effect on multiple organ function damage such as kidney damage, myocardial infarction, and nervous system diseases. They can reverse the trend of cell apoptosis and promote tissue cell damage repair, with no significant difference from maternal cells.

[0004] When using exosomes secreted by MSCs to prepare facial masks, the equipment and processes used are relatively complex, resulting in a long preparation time and high cost for the exosome mask, making it difficult to produce and use on a large scale. Utility Model Content

[0005] The utility model aims to provide a device for preparing a facial mask using mesenchymal stem cell exosomes, so as to solve the problem of high cost in the preparation of existing exosome facial masks.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] The utility model provides a device for preparing a facial mask using mesenchymal stem cell exosomes, comprising: an adsorption body, a plurality of slots arranged inside the adsorption body, and a liquid inlet pipe and a liquid outlet pipe symmetrically arranged on both sides of the adsorption body, wherein the liquid inlet pipe and the liquid outlet pipe are both connected to each of the slots, and the liquid inlet pipe is respectively connected to a supernatant inlet pipe and a physiological saline inlet pipe.

[0008] Preferably, buckles are provided on the liquid outlet tube, the supernatant inlet tube and the physiological saline inlet tube.

[0009] Preferably, a filter screen is provided on the supernatant inlet pipe.

[0010] Preferably, a plurality of the slots are arranged in parallel and connected.

[0011] Preferably, a facial mask cloth is placed in the slot.

[0012] Preferably, the facial mask cloth is a facial mask cloth in which hyperbranched polyamide is loaded on facial mask paper.

[0013] The utility model has the following beneficial effects:

[0014] (1) The device enables the exosome supernatant to flow evenly through the mask cloth and efficiently captures exosomes in the exosome supernatant.

[0015] (2) The device can capture exosomes under sterile conditions, and the preparation of freeze-dried masks is simple, fast, and efficient.

[0016] (3) The device is easy to operate, occupies a small area, and does not require the preparation of other items. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of the structure of a device for preparing a facial mask using mesenchymal stem cell exosomes provided in an embodiment of the present application;

[0018] Symbols represent:

[0019] 1-adsorption body, 2-slot, 3-liquid inlet pipe, 4-liquid outlet pipe, 5-supernatant inlet pipe, 6-physiological saline inlet pipe, 7-clip, 8-filter. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] Please refer to the attached Figure 1 , attached Figure 1 The device for preparing a facial mask using mesenchymal stem cell exosomes provided in the embodiment of the present application is shown. Figure 1 It can be seen that the device for preparing a facial mask using mesenchymal stem cell exosomes provided in the embodiment of the present application includes an adsorption body 1, a slot 2, a liquid inlet pipe 3 and a liquid outlet pipe 4. Among them, the adsorption body 1 is a component for placing the facial mask cloth and the exosome supernatant flowing through, which can be a box-type structure, a bag-type structure, etc. The slot 2 is arranged inside the adsorption body 1 for placing the facial mask cloth. In the embodiment of the present application, a plurality of independent slots 2 are arranged inside the adsorption body 1, and the plurality of independent slots 2 are arranged side by side to ensure that the liquid can pass through each slot 2 evenly.

[0022] The opposite sides of the outside of the adsorption body 1 are respectively provided with a liquid inlet pipe 3 and a liquid outlet pipe 4, and the liquid inlet pipe 3 and the liquid outlet pipe 4 are connected to each slot 2. In addition, the liquid inlet pipe 3 is also connected to the supernatant inlet pipe 5 and the saline inlet pipe 6. Thus, the exosome supernatant and saline are respectively input into the adsorption body 1 through the supernatant inlet pipe 5 and the saline inlet pipe 6, so that the exosome supernatant and saline flow through the mask cloth in the slot 2, so that the mask cloth captures the exosomes. In the embodiment of the present application, the mask cloth is a mask cloth loaded with hyperbranched polyamide on the mask paper, which can effectively capture the exosomes in the exosome supernatant and then attach to the mask paper.

[0023] In addition, a buckle 7 is provided on the liquid outlet tube 4, the supernatant inlet tube 5 and the saline inlet tube 6 to control the inlet and outlet of the liquid through the buckle 7. In the embodiment of the present application, the supernatant inlet tube 5 and the saline inlet tube 6 are opened alternately, which can more effectively improve the capture rate of exosomes. When the buckle 7 is closed, it can ensure that the interior of the adsorption body 1 is sterile to avoid bacterial contamination. Furthermore, a filter screen 8 is provided on the supernatant inlet tube 5, and the buckle 7 is located between the filter screen 8 and the adsorption body 1, so that the impurities in the supernatant of the exosomes are filtered out through the filter screen 8 and then enter the interior of the adsorption body 1.

[0024] When the device provided in the embodiment of the present application is used, the exosome supernatant is connected to the supernatant inlet tube 5, and the physiological saline is connected to the physiological saline inlet tube 6. The buckle 7 on the supernatant inlet tube 5 and the liquid outlet tube 4 is opened, and the buckle 7 on the physiological saline inlet tube 6 is closed. At this time, the exosome supernatant slowly flows through the slot 2 in the adsorption body 1 at a speed of 3mL / min, and then the mask cloth in the slot 2 is completely soaked and discharged through the liquid outlet tube 4, so as to achieve the purpose of capturing exosomes through the mask cloth. After the capture is completed, the buckle 7 on the supernatant inlet tube 5 is closed, and the buckle 7 on the physiological saline inlet tube 6 and the liquid outlet tube 4 is opened. At this time, the physiological saline flows slowly at a speed of 5mL / min and is discharged from the liquid outlet tube 4 to facilitate the washing of the exosome supernatant remaining on the mask cloth. After the washing is completed, all the liquid in the adsorption body 1 flows out, and all buckles 7 are closed. Take out the mask cloth, freeze-dry and package under sterile conditions.

[0025] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A device for preparing a facial mask using mesenchymal stem cell exosomes, characterized in that: include: An adsorption body (1), a plurality of slots (2) arranged inside the adsorption body (1), and a liquid inlet pipe (3) and a liquid outlet pipe (4) symmetrically arranged on both sides of the adsorption body (1), wherein the liquid inlet pipe (3) and the liquid outlet pipe (4) are both connected to each of the slots (2), and the liquid inlet pipe (3) is respectively connected to a supernatant liquid inlet pipe (5) and a physiological saline inlet pipe (6).

2. The device for preparing a facial mask using mesenchymal stem cell exosomes according to claim 1, characterized in that: The liquid outlet tube (4), the supernatant liquid inlet tube (5) and the physiological saline inlet tube (6) are all provided with buckles (7).

3. The device for preparing a facial mask using mesenchymal stem cell exosomes according to claim 1, characterized in that: The supernatant liquid inlet pipe (5) is provided with a filter screen (8).

4. The device for preparing a facial mask using mesenchymal stem cell exosomes according to claim 1, characterized in that: The plurality of slots (2) are arranged in parallel and connected.

5. The device for preparing a facial mask using mesenchymal stem cell exosomes according to claim 1, characterized in that: A facial mask cloth is placed in the slot (2).

6. The device for preparing a facial mask using mesenchymal stem cell exosomes according to claim 5, characterized in that: The facial mask cloth is a facial mask cloth in which hyperbranched polyamide is loaded on facial mask paper.

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