Stem tip slitting device for tissue culture detoxification
By designing a stem tip slitting device for tissue culture detoxification, the accurate alignment and cutting of the stem tip is achieved using the reference line and clamping mechanism, the cutting problem is solved due to hand shaking and the efficiency of tissue culture work is improved.
Patent Information
- Application Number
- CN202421534642.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2025-05-13
- Estimated Expiration
- 2034-07-01
AI Technical Summary
During the detoxification process of tissue culture, due to human hands shaking when slicing the stem tip tissue, it is difficult to accurately cut out tissues of 0.5mm length, which affects the smooth progress of tissue culture work.
A stem tip slitting device for tissue culture detoxification is designed, including a microscope body, a reference line, a jaw mechanism and a clamping mechanism, so as to achieve accurate alignment and cutting of the stem tip through mechanical structure.
The device ensures the stability of the stem tip and the accuracy of the cutting length through the cooperation of the reference line and the clamping mechanism, avoids the cutting inaccurate problem caused by hand shaking, and improves the efficiency of tissue culture work.
Smart Images
Figure CN222858176U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a cutting device, in particular to a stem tip cutting device for tissue culture detoxification. Background Art
[0002] Tissue culture detoxification, i.e. plant tissue culture detoxification technology, is a method widely used for plant virus removal. Based on the uneven distribution of viruses in plants and the omnipotence of plant cells. In tissue cells that divide vigorously and grow rapidly, such as plant stem tips, viruses are often difficult to exist. By using virus-free plant tissues or cells for tissue culture, virus-free plants can be obtained. Stem tip tissue culture detoxification is commonly used in plants such as potatoes, orchids, lilies, irises, and apples. Before culture, it is necessary to cut a 0.5mm tissue from the stem tip of the virus-infected plant, and then culture the cut tissue through culture technology to obtain virus-free plants. However, the length of 0.5mm tissue is very short, and when cutting the tissue, the staff usually needs to cut it with the help of a comparison ruler under a microscope. However, when a person holds the plant in one hand and the knife in the other hand for cutting, the hand often shakes, resulting in inaccurate knife position during cutting, and the length of the cut stem tip tissue is not up to standard (process or too short), which is not conducive to the smooth development of tissue culture. Utility Model Content
[0003] The utility model aims to provide a stem tip cutting device for tissue culture detoxification, which can conveniently cut out plant stem tip tissues with a length of 0.5 mm.
[0004] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0005] A stem tip cutting device for tissue culture detoxification, comprising
[0006] A microscope body, wherein the microscope body has a workbench, a light-transmitting hole is provided in the middle of the workbench, and a reference line is also stretched on the workbench, the reference line extends along the width direction of the workbench and passes through the light-transmitting hole;
[0007] A first adjustment mechanism is provided on one side edge of the workbench and is capable of adjusting the extension length in the direction of the reference line;
[0008] A clamping mechanism, used for clamping the stem tip, wherein the clamping mechanism is connected to the end of the first adjusting mechanism so as to move synchronously with the first adjusting mechanism;
[0009] A second adjustment mechanism is arranged on the side of the workbench opposite to the side where the first adjustment mechanism is located, and the first adjustment mechanism can adjust the extension length in the direction of the reference line;
[0010] The clamping mechanism is used for clamping the slicing tool, and the clamping mechanism is connected to the end of the second adjustment mechanism to drive the slicing tool to cut the stem tip that has been extended into place.
[0011] Preferably, the workbench is provided with a limiting groove extending along the width direction of the workbench, and the reference line is sunken in the limiting groove.
[0012] Preferably, the workbench is further provided with a pair of elastic pressing plates arranged at both ends of the limiting groove, and after the reference line is sunk into the limiting groove, the elastic pressing plates are pressed against the reference line.
[0013] Preferably, a pair of winding rods are also provided on the workbench, and the pair of winding rods are arranged at both ends of the limiting groove, so that the two ends of the reference line trapped in the limiting groove can be wound on the winding rods.
[0014] Preferably, the first adjustment mechanism and the second adjustment mechanism have the same structure, including an upper U-shaped bracket detachably fixed to the workbench, the U-shaped bracket is provided with a threaded sleeve extending along the length direction of the workbench, a screw rod is threaded in the threaded sleeve, an extension rod is rotatably connected to the inner end of the screw rod located in the workbench, a guide groove extending along the axial direction of the screw rod is provided at the lower part of the extension rod, a guide rail is fixedly connected to the threaded sleeve, and the guide rail is slidably disposed in the guide groove.
[0015] Preferably, the clamping mechanism includes a sleeve connected to the extension rod in the first adjustment mechanism, a screw is coaxially arranged in the sleeve, a nut is arranged above the sleeve, the screw is threaded into the nut, a spring is sleeved on the part of the screw located below the sleeve, the spring is press-fitted to apply a downward pulling force to the screw so that the nut has a frictional force against the end face of the sleeve, and a clamp is also connected to the lower end of the screw.
[0016] Preferably, the clamping mechanism includes a rotating shaft extending axially along the extension rod in the second adjustment mechanism, the rotating shaft can be rotatably inserted in the extension rod, and a U-shaped clamping seat is connected to the outer end of the rotating shaft extending out of the extension rod, and a locking bolt is screwed on the clamping seat. The locking bolt is rotated to make the end face of the locking bolt abut against the handle of the slicing tool placed in the clamp, and a torsion spring is also sleeved on the rotating shaft, one end of the torsion spring is connected to the extension rod, and the other end is connected to the clamping seat, so that the rotating shaft can be reset to an initial state after the external force is lost.
[0017] Compared with the prior art, the beneficial effects of the utility model are:
[0018] 1. In this solution, by using the reference line and the clamping mechanism connected to the first adjustment mechanism, the tip of the stem apex that needs to be sliced can be accurately aligned on one side of the reference line, and the stability of the stem apex can be maintained when slicing the stem apex. The clamping mechanism connected to the second adjustment mechanism can accurately move the slicing tool to a position that deviates from the reference line by the required distance (stem apex segment length), thereby achieving accurate segmentation of the stem apex in the subsequent process. It should be noted that the above-mentioned stem apex segmentation operations are all implemented through a mechanical structure to avoid jitter during segmentation, thereby effectively ensuring the accuracy of the segment length.
[0019] 2. Set a limit groove and trap the reference line in the limit groove to prevent the reference line from affecting the slide on the workbench. In addition, the reference line is trapped in the limit groove and can be guided by the limit groove to ensure that the extension direction of the reference line can be maintained in the width direction of the workbench.
[0020] 3. An elastic pressing piece is provided to prevent the reference line trapped in the limiting groove from escaping from the limiting groove and running onto the upper surface of the workbench.
[0021] 4. Set up a winding rod. When the two ends of the reference line are respectively wound on the winding rod, the precision line can be kept taut at all times when the stem tip is cut.
[0022] 5. The structure adopted by the first adjustment mechanism and the second adjustment mechanism adopts a screw rod to adjust the telescopic length, which has the advantages of high accuracy and simple operation, and greatly facilitates the staff to accurately adjust the position of the stem tip clamped by the clamping mechanism and the slitting tool clamped by the clamping mechanism when slitting.
[0023] 6. In this solution, the structure of the clamping claw mechanism can make certain rotation adjustments according to the direction of the stem tip after clamping the stem tip. The extension direction of the tip part of the plant stem tip temporarily remains basically consistent with the extension direction of the first adjustment mechanism, thereby achieving precise adjustment of the position of the stem tip part and facilitating the subsequent slicing tool to cut the tip part of the stem tip.
[0024] 7. The clamping mechanism in this solution can achieve precise position adjustment through the second adjustment mechanism connected thereto, so that the clamped slicing tool can be accurately moved to the position. At the same time, the blade is fixed on a rotatable clamping seat, so when performing stem tip slicing, it is only necessary to gently move the handle of the slicing tool, which is convenient and quick. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a schematic diagram of the structure of the utility model;
[0026] Figure 2 It is a cross-sectional view of the utility model from the front side perspective;
[0027] Figure 3 It is a cross-sectional view of the utility model from the rear side perspective;
[0028] Figure 4 It is a cross-sectional view of the utility model from a top view perspective.
[0029] Reference numerals:
[0030] 100. Microscope body 110. Workbench 120. Light transmission hole 130. Limiting groove
[0031] 200. First adjustment mechanism 210. U-shaped holder 220. Sleeve 230. Screw 240. Extension rod 241. Guide groove 250. Guide rail
[0032] 300. Clamp mechanism 310. Sleeve 320. Nut 330. Screw 340. Spring 350. Clip
[0033] 400. Second adjustment mechanism
[0034] 500. Clamping mechanism 510. Torsion spring 520. Rotating shaft 530. Clamping seat 540. Locking bolt
[0035] 600. Elastic tableting
[0036] 700. Baseline
[0037] 800. Winding rod DETAILED DESCRIPTION
[0038] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0039] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.
[0040] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0041] like Figures 1 to 4 A stem apex cutting device for tissue culture detoxification is shown, which includes a microscope body 100 for auxiliary observation and positioning during cutting, and a workbench 110 for placing a slide is provided on the microscope body, and a light-transmitting hole is provided at the center of the workbench so that the light on the reflector below can be irradiated through the light-transmitting hole to the fabric stem apex part on the slide. It should be noted that in order to achieve accurate control of the length of the stem apex cutting part under the microscope environment, when adjusting the stem apex tissue on the slide and the cutting blade for cutting the stem apex tip part, a reference for zeroing the above two is required. Specifically, in this solution, a reference line 700 is provided on the workbench 110, and the reference line extends straight along the width direction of the workbench and is provided through the light-transmitting hole, so that when observing the stem apex tissue on the slide through the microscope, the reference line located below the slide can be observed synchronously. It should be noted that, in the present solution, the reference line is made of carbon fiber wire with a diameter of 0.05 to 0.01 mm to ensure that the reference line will not break in a stretched state.
[0042] It should also be noted that the reference line, as the zero line and calibration line during slitting, must remain stable and extend along the width direction of the workbench during the slitting operation. To this end, as a lifting solution, a downwardly recessed limit groove 130 can also be opened on the workbench, and the limit groove is set through the light-transmitting hole. When laying the reference line, the reference line can be sunken into the limit groove to prevent the reference line from rolling on the workbench when the slide is fine-tuned. In addition, when the reference line is sunken into the limit groove, the slide will also be out of contact with the reference line to prevent the reference line from being cut by the sharp edge of the slide.
[0043] It should also be noted that, in order to prevent the reference line from escaping from the limiting groove, a set of elastic pressing sheets 600 can be provided at both ends of the limiting groove 130. The set of elastic pressing sheets has two pieces in total and is provided at both ends of the limiting groove. The elastic pressing sheet has a V-shaped structure, and its bottom enters the limiting groove to press the reference line placed in the limiting groove. In addition, the V-shaped structure also facilitates the reference line to slide from the bottom of the elastic pressing sheet into the limiting groove.
[0044] It should also be noted that if Figure 4 As shown, a group of winding rods 800 are also arranged on both sides of the workbench. This group of winding rods also has two rods, and are respectively arranged at the two ends of the limit groove. After the two ends of the baseline extend out of the limit groove, they can also be wound on the winding rods to further improve the stability of the baseline.
[0045] like Figure 4 As shown, a first adjustment mechanism 200 and a second adjustment mechanism 400 are respectively provided on both sides of the workbench in the length direction. It should be noted that the first adjustment mechanism and the second adjustment mechanism have the same structure and can be extended and retracted in the direction of the reference line.
[0046] It should be noted that if Figure 2 As shown, the common structure of the first adjustment mechanism and the second adjustment mechanism includes a U-shaped holder 210, which is clamped on the side edge of the length direction of the workbench, and then the position on the workbench is fixed by a bolt on the U-shaped holder. At the same time, a threaded sleeve 220 is also fixedly connected above the U-shaped holder, and the threaded sleeve extends along the length direction of the workbench to achieve verticality with the reference line. A screw rod 230 is screwed in the threaded sleeve, and it should be noted that the cooperation between the screw rod and the threaded sleeve is that the screw rod is rotated one circle, and the screw rod moves axially in the threaded sleeve by 0.05mm. At the same time, an extension rod 240 is also connected to the end face of the screw rod located in the workbench, and the extension rod is coaxially rotatably connected with the screw rod to ensure that when the screw rod rotates, the extension rod can be kept from rotating by limiting the extension rod. Specifically, a guide groove 241 extending along the axial direction of the screw rod is provided below the extension rod, and a guide rail 250 is provided on the screw sleeve, which is slidably inserted in the guide groove, so that the extension rod can make synchronous axial movement with the screw rod, and can rotate independently when the screw rod rotates.
[0047] like Figure 2As shown, a group of clamping mechanisms 300 are connected to the first adjustment mechanism. After the clamping mechanism is connected to the first adjustment mechanism, it follows the first adjustment mechanism to move synchronously close to or away from the reference line under the drive of the first adjustment mechanism. It should be noted that the clamping mechanism is used to clamp the plant stem tip tissue after peeling, and move the tip position of the stem tip to the reference line position through the first adjustment mechanism. Specifically, the clamping mechanism includes a sleeve 310 connected to the extension rod, the sleeve extends vertically, a nut 320 is arranged above the sleeve, and a screw rod 330 is also inserted in the sleeve in a sliding fit. It should be noted that the upper part of the screw rod is screwed into the nut, and a spring 340 is sleeved on the lower end of the screw rod extending out of the sleeve. The spring is in a compressed state, and the upper end abuts on the lower end surface of the sleeve, and the lower end is connected to the screw rod. At this time, the compressed spring applies a downward pulling force to the screw rod, so that the nut is pulled down to abut on the upper end surface of the sleeve. At the same time, if Figure 2 As shown, a clamp 350 for clamping the stem of a plant is fixedly connected to the lower end of the screw. It should be noted that in this solution, because the upper end of the spring is not fixedly connected to the sleeve, the screw can achieve relative rotation in the sleeve under the torsion of an external force, and the clamp also rotates accordingly. Through the above adjustment, the stem tip of the plant stem clamped by the clamp can be perpendicular to the reference line. At this time, when the first adjustment mechanism is adjusted, the stem tip can also move vertically toward the direction of the reference line.
[0048] In addition, it should be noted that a clamping mechanism 500 is connected to the end of the extension rod in the second adjustment mechanism. The clamping mechanism is used to clamp the slicing tool and can be moved to an accurate position (a distance of the stem tip length required by the reference line, such as 0.5 mm) under the adjustment of the second adjustment mechanism, so as to implement slicing of the stem tip extended to the zero position.
[0049] It should be noted that in order to ensure the smooth progress of slitting, the clamping mechanism in this scheme is as follows Figure 3 As shown, it includes a rotating shaft 520, which is rotatably inserted into the extension rod of the second adjustment mechanism 400. At the same time, a torsion spring 510 is sleeved on the part of the rotating shaft extending from the extension rod, one end of the torsion spring is fixedly connected to the extension rod, and the other end of the torsion spring is connected to the end of the rotating shaft, so that the rotating shaft can be reset after rotating under the action of external force. In addition, as Figure 3As shown, a U-shaped clamping seat 530 is connected to the outer end of the rotating shaft, and a locking bolt 540 is screwed on the outer surface of the clamping seat. When the handle of the slitting tool is placed in the groove in the clamping seat, the locking bolt is rotated so that the end of the locking bolt abuts against the side of the slitting tool, thereby fixing the slitting tool on the clamping seat. When it is necessary to slit the stem tip tissue, the staff only needs to push the slitting tool handle exposed outside the clamping seat upwards, and the blade part of the handle is turned down to cut the stem tip of the plant into a desired length of tissue.
[0050] Working principle: When it is necessary to cut the plant stem tip, the staff first straightens the reference line into the limit groove, and then winds the two ends on the winding rod. Then, place the glass slide on the workbench and clamp it. When the above steps are completed, the staff clamps the peeled plant stem with the clamp in the clamp mechanism, and then turns the bolt to adjust the downward height of the screw rod, so that the bent plant stem fits the surface of the glass slide as much as possible. While turning the nut, the screw rod is also turned at the same time, so that the tip of the plant stem clamped by the clamp is as perpendicular to the direction of the reference line as possible. After finding the stem tip of the plant through a microscope, turn the screw rod in the first adjustment mechanism to move the stem tip of the plant toward the direction of the reference line until the end of the stem tip of the plant moves to the reference line. Then, the staff resets the second adjustment mechanism to zero, and the blade of the slitting tool clamped by the clamping mechanism is aligned with the reference line. Afterwards, the staff uses a microscope to confirm whether the slitting tool is accurately aligned with the reference line. If not, the screw in the second adjustment mechanism is rotated so that the blade is facing the reference line. When the above calibration steps are completed, the staff rotates the screw according to the required length of the plant stem tip. For example, if the required length of the stem tip tissue is 0.5mm, the staff can rotate the screw ten times. When the number of turns is sufficient, the staff can move the handle of the slitting tool clamped by the clamping seat. At this time, the slitting tool turns down and accurately cuts on the stem tip of the plant, and accurately cuts out the stem tip tissue of the required length. It should be noted that in the above scheme, when the stem tip is cut, the grip of the plant neck and the grip of the tool are both achieved through a mechanical structure, which effectively avoids the situation where the cutting length is inaccurate due to shaking when the hand is held for slitting.
[0051] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A stem tip cutting device for tissue culture detoxification, characterized in that: include A microscope body (100), the microscope body (100) having a workbench (110), a light-transmitting hole (120) being provided in the middle of the workbench (110), a reference line (700) being arranged in a straight line on the workbench (110), the reference line (700) extending along the width direction of the workbench (110) and passing through the light-transmitting hole (120); A first adjustment mechanism (200) is disposed on a side edge of the workbench (110) and is capable of adjusting the extension length in the direction of the reference line (700); A clamping claw mechanism (300) is used to clamp the stem tip, and the clamping claw mechanism (300) is connected to the end of the first adjustment mechanism (200) so as to move synchronously with the first adjustment mechanism (200); A second adjustment mechanism (400) is arranged on a side of the workbench (110) opposite to the side where the first adjustment mechanism is located, and the first adjustment mechanism (200) can adjust the extended length in the direction of the reference line (700); The clamping mechanism (500) is used to clamp the slicing tool, and the clamping mechanism (500) is connected to the end of the second adjustment mechanism (400) to drive the slicing tool to cut the stem tip that has been extended into place.
2. A stem tip cutting device for tissue culture detoxification as claimed in claim 1, characterized in that: The workbench (110) is provided with a limiting groove (130) extending along the width direction of the workbench (110), and the reference line (700) is set in the limiting groove (130).
3. A stem tip cutting device for tissue culture detoxification as claimed in claim 2, characterized in that: The workbench (110) is also provided with a pair of elastic pressing sheets (600) arranged at both ends of the limiting groove (130); after the reference line (700) is sunk into the limiting groove (130), the elastic pressing sheet (600) is pressed against the reference line (700).
4. A stem tip cutting device for tissue culture detoxification as claimed in claim 3, characterized in that: A pair of winding rods (800) are also provided on the workbench (110), and the pair of winding rods (800) are arranged at both ends of the limiting groove (130) so that both ends of the reference line (700) trapped in the limiting groove (130) can be wound around the winding rods (800).
5. A stem tip cutting device for tissue culture detoxification as claimed in claim 1, characterized in that: The first adjustment mechanism (200) and the second adjustment mechanism (400) have the same structure, including an upper U-shaped holder (210) detachably fixed to the workbench (110), the U-shaped holder (210) is provided with a threaded sleeve (220) extending along the length direction of the workbench (110), a screw rod (230) is screwed in the threaded sleeve (220), an extension rod (240) is rotatably connected to the inner end of the screw rod (230) located in the workbench (110), a guide groove (241) extending along the axial direction of the screw rod (230) is provided at the lower part of the extension rod (240), a guide rail (250) is fixedly connected to the threaded sleeve (220), and the guide rail (250) is slidably arranged in the guide groove (241).
6. A stem tip cutting device for tissue culture detoxification as claimed in claim 5, characterized in that: The clamp mechanism (300) comprises a sleeve (310) connected to an extension rod (240) in the first adjustment mechanism (200); a screw rod (330) is coaxially arranged in the sleeve (310); a nut (320) is arranged above the sleeve (310); the screw rod (330) is threadedly connected to the nut (320); a spring (340) is sleeved on the portion of the screw rod (330) located below the sleeve (310); the spring (340) is press-fitted to apply a downward pulling force to the screw rod (330) so that the nut (320) is frictionally abutted against the end surface of the sleeve (310); and a clamp (350) is also connected to the lower end of the screw rod (330).
7. A stem tip cutting device for tissue culture detoxification as claimed in claim 5, characterized in that: The clamping mechanism (500) comprises a rotating shaft (520) extending axially along the extension rod (240) in the second adjustment mechanism (400); the rotating shaft (520) is rotatably inserted into the extension rod (240); a U-shaped clamping seat (530) is connected to the outer end of the rotating shaft (520) extending out of the extension rod (240); a locking bolt (540) is screwed on the clamping seat (530); the locking bolt (540) is rotated so that the end surface of the locking bolt (540) abuts against the handle of the slicing tool inserted into the clamping; a torsion spring (510) is also sleeved on the rotating shaft (520); one end of the torsion spring (510) is connected to the extension rod (240), and the other end is connected to the clamping seat (530), so that the rotating shaft (520) can be reset to an initial state after the external force is removed.