Plant explant sterilization treatment device

Abstract

The utility model relates to the technical field of plant tissue culture, and provides a plant explant sterilization treatment device, which comprises a tank body, a liftable mounting seat is arranged in the tank body, an annular piece capable of reciprocating is arranged on the mounting seat, a rotatable bearing disc is arranged in the annular piece, and the bearing disc is arranged on the mounting seat. A plurality of placing net cylinders for placing explants are uniformly arranged on the bearing disc in the circumferential direction, and a cylinder cover is arranged on each placing net cylinder; in the soaking and sterilizing process, the bearing disc rotates, and meanwhile, the annular piece reciprocates, so that the multiple placement net cylinders are in a revolution and reciprocating state, and the sterilizing solution is in a surging state, and therefore, all parts of the plant explant are fully and comprehensively infiltrated by the sterilizing solution, the problem of a sterilizing blind area is effectively solved, and the sterilizing effect of the plant explant is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of plant tissue culture, in particular to a plant explant sterilization treatment device. Background Art

[0002] In plant tissue culture technology, explants are important in vitro culture materials, and their sterile state is one of the key factors to ensure successful culture.

[0003] Existing plant explant sterilization treatment methods generally adopt the immersion method, that is, the explant to be sterilized is completely immersed in a sterilization solution, and the sterilization effect is achieved by leaving it to soak for a certain period of time. However, the static immersion method often makes it difficult to ensure that the interior of each part of the explant is fully infiltrated with the sterilization solution. The surface of the explant may be irregular in shape, rough in surface, or have tiny gaps, making it difficult for the sterilization solution to penetrate completely. Or when the explant has a complex porous structure, such as the intervening spaces between leaves and the lenticels of stem segments, these parts often become blind spots for sterilization, affecting the final sterilization effect.

[0004] Therefore, the utility model proposes a plant explant sterilization treatment device to solve the above problems. Utility Model Content

[0005] The embodiment of the utility model aims to provide a plant explant sterilization treatment device to solve the above-mentioned problem.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A plant explant sterilization treatment device comprises a tank body, wherein a liftable mounting seat is arranged in the tank body, a reciprocating annular member is arranged on the mounting seat, a rotatable carrying plate is arranged in the annular member, a plurality of placing net cylinders for placing explants are evenly arranged circumferentially on the carrying plate, and each of the placing net cylinders is equipped with a cylinder cover.

[0008] In an optional solution: a rotatable threaded rod is provided in the tank body, the threaded rod is threadedly fitted through the mounting seat, and one side of the mounting seat is in contact with the inner wall of the tank body.

[0009] In an optional solution: a grip sleeve is provided at the upper end of the threaded rod for an operator to grip and rotate the threaded rod.

[0010] In an optional solution: a slide groove is provided on the mounting seat, a slider and a compression spring with one end abutting against the slider are clamped in the slide groove, the slider is fixedly connected to the annular member, a plurality of protrusions are evenly arranged circumferentially on the bottom side of the inner part of the tank body, and at least one roller is provided on the bottom side of the supporting plate for cyclically and alternately abutting against the plurality of protrusions when the supporting plate rotates.

[0011] In an optional solution: a rotatable rotating rod is provided at a position vertically opposite to the center of the supporting plate on the bottom side of the tank body, the rotating rod has a square radial cross-section, a through hole matching the rotating rod is provided at the center of the supporting plate, the rotating rod passes through the supporting plate through the through hole, and a driving member for driving the rotating rod to rotate is provided at the bottom of the tank body.

[0012] Compared with the prior art, the beneficial effects of the embodiments of the present invention are as follows:

[0013] During the immersion sterilization process, the carrier plate rotates and the annular member reciprocates, so that the multiple placed net cylinders are in a state of revolution and reciprocation, and the sterilization solution is in a surging state, so that the sterilization solution can fully and comprehensively infiltrate all parts of the plant explants, effectively solving the problem of sterilization blind spots and ensuring the sterilization effect of the plant explants.

[0014] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings herein are incorporated into and constitute a part of the specification to illustrate embodiments consistent with the present application and are used together with the specification to explain the principles of the present application. At the same time, these drawings and text descriptions are not intended to limit the scope of the concept of the present application in any way, but to illustrate the concept of the present application for those skilled in the art by referring to specific embodiments.

[0016] Figure 1 It is a structural schematic diagram of an embodiment of the utility model.

[0017] Figure 2 It is a schematic diagram of the arrangement of the annular member, the carrying plate and a plurality of net cylinders placed therebetween in an embodiment of the present utility model.

[0018] Figure 3 It is a schematic diagram of the arrangement between the tank body and a plurality of protrusions in an embodiment of the utility model.

[0019] Figure 4 It is a schematic diagram of the arrangement between the mounting seat, the compression spring and the slider in the embodiment of the utility model.

[0020] Notes on the figure numbers: 1-tank body, 2-rotating rod, 3-ring member, 4-carrying plate, 5-net cylinder, 6-mounting seat, 7-roller, 8-threaded rod, 9-bump, 10-driving member, 11-slide groove, 12-compression spring, 13-slider, 14-cylinder cover. DETAILED DESCRIPTION

[0021] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0022] See also Figure 1 and Figure 2 A plant explant sterilization treatment device includes a tank body 1, a liftable mounting seat 6 is provided in the tank body 1, a reciprocating ring member 3 is provided on the mounting seat 6, a rotatable carrying plate 4 is provided in the ring member 3, a plurality of placing net cylinders 5 for placing explants are evenly arranged circumferentially on the carrying plate 4, and each of the placing net cylinders 5 is provided with a cylinder cover 14.

[0023] During sterilization, first add an appropriate amount of sterilization solution into the tank body 1. At this time, the mounting seat 6 is in the upper position of the tank body 1, and multiple placement net tubes 5 are above the liquid surface of the sterilization solution. Then, the plant explants to be sterilized are placed in the placement net tubes 5 and covered with the tube cover 14 (to prevent the plant explants from floating). After the plant explants are placed, the mounting seat 6 is lowered to drive the annular member 3 to lower until the multiple placement net tubes 5 are completely immersed in the sterilization solution. During the immersion sterilization process, the supporting plate 4 rotates, and the annular member 3 moves back and forth, so that the multiple placement net tubes 5 are in a state of revolution and reciprocation, and the sterilization solution is in a surging state, so that the sterilization solution fully and comprehensively infiltrates all parts of the plant explants, effectively solving the problem of sterilization blind spots, and ensuring the sterilization effect of the plant explants.

[0024] Furthermore, a drain port for discharging the sterilizing solution is provided on one side of the bottom of the tank body 1 .

[0025] See also Figure 1 In one embodiment of the utility model, a rotatable threaded rod 8 is provided in the tank body 1 , and the threaded rod 8 is threadedly fitted through the mounting seat 6 , and one side of the mounting seat 6 is in contact with the inner wall of the tank body 1 .

[0026] In this embodiment, the threaded rod 8 is rotated to drive the mounting seat 6 to rise and fall, thereby realizing that a plurality of placement net cylinders 5 are immersed in the sterilization solution and removed from the sterilization solution.

[0027] Furthermore, in this embodiment, a grip sleeve is provided at the upper end of the threaded rod 8 for an operator to grip and rotate the threaded rod 8 .

[0028] See also Figure 1 , Figure 3 and Figure 4In one embodiment of the utility model, a slide groove 11 is provided on the mounting seat 6, a slider 13 and a compression spring 12 with one end abutting against the slider 13 are clamped in the slide groove 11, the slider 13 is fixedly connected to the annular member 3, a plurality of protrusions 9 are evenly arranged on the circumferential bottom side of the tank body 1, and at least one roller 7 is provided on the bottom side of the carrying plate 4 for cyclically and alternately abutting against the plurality of protrusions 9 when the carrying plate 4 rotates.

[0029] In this embodiment, when the supporting plate 4 rotates, it drives the roller 7 to cyclically and alternately abut against the multiple protrusions 9. When the roller 9 abuts against the protrusion 9, it drives the annular member 3, the supporting plate 4 and the multiple placed net cylinders 5 to move upward as a whole, that is, the slider 13 moves upward along the slide groove 11. When the roller 9 is "separated" from the protrusion 9, under the action of the compression spring 12, the slider 13 moves downward along the slide groove 11, so that the annular member 3, the supporting plate 4 and the multiple placed net cylinders 5 move downward as a whole, and this cycle is carried out to realize the reciprocating movement of the multiple placed net cylinders 5.

[0030] See also Figure 1 and Figure 2 In one embodiment of the utility model, a rotatable rotating rod 2 is provided at a position perpendicularly opposite to the center of the carrying plate 4 on the bottom side of the inner part of the tank body 1. The radial cross-section of the rotating rod 2 is square. A through hole adapted to the rotating rod 2 is provided at the center of the carrying plate 4. The rotating rod 2 passes through the carrying plate 4 through the through hole. A driving member 10 for driving the rotating rod 2 to rotate is provided at the bottom of the tank body 1 (the driving member 10 is a rotating device such as a motor and a reduction motor in the prior art, which belongs to the existing mature technology, so it is not described here).

[0031] In this embodiment, the driving member 10 drives the rotating rod 2 to rotate. Since the radial cross-section of the rotating rod 2 is square, the rotation of the rotating rod 2 can drive the supporting plate 4 to rotate without affecting the overall up and down reciprocating movement of the annular member 3, the supporting plate 4 and the multiple net-placing cylinders 5.

[0032] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A plant explant sterilization treatment device, comprising a tank (1), characterized in that: The tank body (1) is provided with a liftable mounting seat (6), the mounting seat (6) is provided with a reciprocating annular member (3), the annular member (3) is provided with a rotatable carrying plate (4), a plurality of placement net cylinders (5) for placing explants are evenly arranged circumferentially on the carrying plate (4), and each of the placement net cylinders (5) is provided with a cylinder cover (14).

2. The plant explant sterilization treatment device according to claim 1, characterized in that: A rotatable threaded rod (8) is provided in the tank body (1), and the threaded rod (8) is threadably fitted through the mounting seat (6), and one side of the mounting seat (6) is in contact with the inner wall of the tank body (1).

3. The plant explant sterilization treatment device according to claim 2, characterized in that: The upper end of the threaded rod (8) is provided with a grip sleeve for an operator to grip and rotate the threaded rod (8).

4. The plant explant sterilization treatment device according to claim 1, characterized in that: The mounting seat (6) is provided with a slide groove (11), a slider (13) and a compression spring (12) with one end abutting against the slider (13) are arranged in the slide groove (11), the slider (13) is fixedly connected to the annular member (3), a plurality of protrusions (9) are evenly arranged on the circumferential bottom side of the inner part of the tank body (1), and at least one roller (7) is arranged on the bottom side of the supporting plate (4) for cyclically and alternately abutting against the plurality of protrusions (9) when the supporting plate (4) rotates.

5. The plant explant sterilization treatment device according to claim 4, characterized in that: A rotatable rotating rod (2) is provided at a position perpendicularly opposite to the center of the supporting plate (4) on the inner bottom side of the tank body (1); the radial cross section of the rotating rod (2) is square; a through hole matching the rotating rod (2) is provided at the center of the supporting plate (4); the rotating rod (2) passes through the supporting plate (4) through the through hole; and a driving member (10) for driving the rotating rod (2) to rotate is provided at the bottom of the tank body (1).

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