Membrane washing device for immunoblotting and immunoblotting membrane washing assembly

Abstract

The utility model discloses an immunoblotting membrane washing device and an immunoblotting membrane washing assembly, the immunoblotting membrane washing device comprises a membrane washing tank, the membrane washing tank is vertically arranged, the opening of the membrane washing tank is upward, the inner lower end of the membrane washing tank is horizontally provided with a supporting plate, the supporting plate divides the interior of the membrane washing tank into an upper tank chamber and a lower chamber, the upper tank chamber is located above the lower chamber, and the lower chamber is located above the lower chamber. A liquid seepage hole for communicating the upper tank chamber with the lower chamber is formed in the supporting plate, a liquid inlet communicated with the interior of the lower chamber is formed in the lower end of the membrane washing tank, and an overflow port communicated with the interior of the upper tank chamber is formed in the upper end of the membrane washing tank, so that membrane washing liquid can be introduced into the lower chamber through the liquid inlet and then overflows into the upper tank chamber from the lower chamber through the liquid seepage hole; the membrane is arranged in the upper groove chamber, and the liquid level in the upper groove chamber can overflow after reaching the position of the overflow port, so that the membrane washing liquid in the upper groove chamber flows from bottom to top and is updated in real time to wash the membrane material, and the cleaning effect is good.

Description

Technical Field

[0001] The utility model belongs to the field of biochemical experimental instruments, and particularly relates to an immunoblotting membrane washing device and an immunoblotting membrane washing assembly. Background Art

[0002] As a method for qualitative and quantitative analysis of proteins, immunoblotting requires washing after antibody incubation to remove nonspecific reactions other than the target antigen and antibody, thereby improving the accuracy of detection. Currently, there are many devices related to membrane washing, but the number of membranes washed is limited. When there are many parallel experiments, the membrane washing efficiency is relatively low. Although the document number CN218251828U "An automatic membrane washing machine for protein immunoblotting" can also wash multiple membranes, its structure is too complicated. Utility Model Content

[0003] In order to solve the above technical problems, the purpose of the utility model is to provide a membrane washing device for immunoblotting which has a simple structure and can continuously and slowly wash the membrane for immunoblotting.

[0004] In order to achieve the above-mentioned purpose, the technical solution of the utility model is as follows: a membrane washing device for immunoblotting, comprising a membrane washing tank, which is vertically arranged with its notch facing upward, and a support plate is horizontally provided at the inner lower end of the membrane washing tank, and the support plate divides the membrane washing tank into an upper chamber and a lower chamber, the upper chamber is located above the lower chamber, and the support plate is provided with a seepage hole connecting the upper chamber and the lower chamber, the lower end of the membrane washing tank is provided with a liquid inlet connected to the lower chamber, and the upper end of the membrane washing tank is provided with an overflow port connected to the upper chamber.

[0005] The beneficial effect of the above technical solution is that the membrane washing liquid can be introduced into the lower chamber through the liquid inlet, and then overflow from the lower chamber through the seepage hole into the upper chamber, and the membrane is placed in the upper chamber. After the liquid level in the upper chamber reaches the overflow port, it will overflow, so that the membrane washing liquid in the upper chamber flows from bottom to top and is updated in real time to wash the membrane material; of course, during washing, the entire membrane washing tank can be placed on a cyclotron oscillator, so that the membrane material is cleaned under vibration conditions, and the cleaning effect is good.

[0006] The above technical solution also includes a liquid collecting tank, which is arranged on one side corresponding to the length direction of the membrane washing tank, and the overflow port is connected to the inside of the liquid collecting tank. The lower end of the liquid collecting tank is provided with a drainage port connected to the inside thereof.

[0007] The beneficial effect of the above technical solution is that: by setting a liquid collecting tank, the membrane washing liquid overflowing from the overflow port is collected and discharged in a centralized manner, so that the drainage effect is better.

[0008] In the above technical solution, the liquid collecting tank is arranged at the upper end of the corresponding side of the membrane washing tank.

[0009] The beneficial effect of the above technical solution is that the volume of the liquid collecting tank is relatively small, and it is convenient to set a liquid discharge port at the lower end thereof, which makes it easier to connect the pipeline.

[0010] In the above technical solution, there is a height difference between the overflow port and the inner bottom wall of the liquid collecting tank.

[0011] The beneficial effect of the above technical solution is that it can avoid the overflow port being flush with the inner bottom wall of the liquid collecting tank to cause interference in liquid discharge.

[0012] The above technical solution also includes a plurality of partitions, which are all vertically arranged in the upper tank chamber, and the plurality of partitions are parallel to each other in the upper tank chamber to divide the upper tank chamber into a plurality of sub-chambers along its length direction, a plurality of seepage holes are arranged on the support plate, and each sub-chamber corresponds to at least one seepage hole, and a plurality of overflow ports are arranged, and each sub-chamber corresponds to one overflow port.

[0013] The beneficial effect of the above technical solution is that: in this way, a plurality of sub-chambers are separated in the upper chamber, so that more membrane materials can be cleaned at the same time without interfering with each other.

[0014] The horizontal height of the upper end of the partition in the above technical solution is lower than the horizontal height of the notch of the membrane washing tank.

[0015] The beneficial effect of the above technical solution is that a buffer zone is formed in the upper chamber above the plurality of sub-chambers to prevent any sub-chamber from being full of liquid and overflowing through the slot of the upper chamber.

[0016] The width of the sub-groove chamber in the above technical solution is 2-5 mm.

[0017] The beneficial effect of the above technical solution is that the membrane material can be placed upright in the sub-slot chamber, which makes the membrane washing effect better.

[0018] The above technical solution also includes a slot cover, the slot cover has a slot facing downward and is detachably buckled at the slot of the membrane washing tank, and a ventilation hole is arranged on the top wall of the slot cover.

[0019] The beneficial effect of the above technical solution is that when washing the membrane, the tank cover can be placed at the notch of the membrane washing tank, so as to prevent the membrane washing liquid from shaking out.

[0020] The second purpose of the utility model is to provide an immunoblotting membrane washing assembly which has a simple structure and can perform membrane washing treatment on immunoblotting membrane materials.

[0021] In order to achieve the above-mentioned purpose, the technical solution of the utility model is as follows: a immunoblotting membrane washing assembly, comprising a liquid storage bottle, a waste liquid collection bottle, a liquid supply tube, a liquid discharge tube and the immunoblotting membrane washing device as described above, the liquid storage bottle is arranged above the immunoblotting membrane washing device, one end of the liquid supply tube is connected to the liquid storage bottle, and the other end of the liquid supply tube is connected to the liquid inlet, the waste liquid collection bottle is placed below the immunoblotting membrane washing device, one end of the liquid discharge tube is connected to the liquid discharge port, and the other end of the liquid discharge tube is connected to the waste liquid collection bottle.

[0022] The beneficial effect of the above technical solution is that the membrane washing liquid in the liquid storage bottle can be stably supplied to the lower chamber through the liquid supply pipe, and the membrane washing liquid to be discharged from the liquid collecting tank is discharged into the waste liquid collection bottle through the drain pipe, thereby achieving the membrane material washing by the membrane washing liquid in a flowing and renewed state.

[0023] The liquid supply pipe in the above technical solution is a siphon tube, the upper end of which extends into the inner bottom of the liquid storage bottle.

[0024] The beneficial effect of the above technical solution is that the supply of membrane washing liquid is more stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is one of the cross-sectional views of the membrane washing device for immunoblotting described in Example 1 of the utility model;

[0026] Figure 2 This is the second cross-sectional view of the membrane washing device for immunoblotting described in Example 1 of the utility model;

[0027] Figure 3 A top view of the membrane washing device for immunoblotting described in Example 1 of the utility model;

[0028] Figure 4 This is a side view of the membrane washing device for immunoblotting described in Example 1 of the utility model;

[0029] Figure 5 This is a schematic structural diagram of the immunoblotting membrane washing assembly described in Example 2 of the present invention.

[0030] In the figure: 1 membrane washing tank; 11 upper tank chamber; 111 overflow port; 112 sub-tank chamber; 12 lower chamber; 121 liquid inlet; 2 support plate; 21 liquid seepage hole; 3 liquid collecting tank; 31 liquid discharge port; 4 partition plate; 5 tank cover; 51 air vent; 10 membrane washing device for immunoblotting; 20 liquid storage bottle; 30 waste liquid collection bottle; 40 liquid supply pipe; 50 liquid discharge pipe. DETAILED DESCRIPTION

[0031] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention. The present invention is described in more detail in the following paragraphs by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become clearer according to the following description and claims. It should be noted that the drawings are all in a very simplified form and are not in precise proportions, and are only used to conveniently and clearly assist in explaining the purpose of the embodiments of the present invention.

[0032] Example 1

[0033] like Figure 1-Figure 3 As shown, this embodiment provides a membrane washing device for immunoblotting, including a membrane washing tank 1, wherein the membrane washing tank 1 is vertically arranged with a notch facing upward, a support plate 2 is horizontally arranged at the inner lower end of the membrane washing tank 1, and the support plate 2 divides the membrane washing tank 1 into an upper tank chamber 11 and a lower chamber 12, wherein the upper tank chamber 11 is located above the lower chamber 12, and a liquid seepage hole 21 is arranged on the support plate 2 for connecting the upper tank chamber 11 with the lower chamber 12, and a liquid inlet 121 is arranged at the lower end of the membrane washing tank 1 to connect the lower chamber 12. The upper end of the membrane washing tank 1 is provided with an overflow port 111 connected to the upper tank chamber 11, so that the membrane washing liquid can be introduced into the lower chamber through the liquid inlet, and then overflow from the lower chamber into the upper tank chamber through the seepage hole, and the membrane is placed in the upper tank chamber. After the liquid level in the upper tank chamber reaches the position of the overflow port, it will overflow, so that the membrane washing liquid in the upper tank chamber flows from bottom to top and is updated in real time to wash the membrane material; of course, during washing, the entire membrane washing tank can be placed on a cyclotron oscillator, so that the membrane material is cleaned under vibration conditions, and the cleaning effect is good.

[0034] like Figure 2-Figure 4 As shown, the above technical solution also includes a liquid collecting tank 3, which is arranged on a side corresponding to the length direction of the membrane washing tank 1, and the overflow port 111 is connected to the inside of the liquid collecting tank 3, and the lower end of the liquid collecting tank 3 is provided with a discharge port 31 connected to the inside thereof. By setting the liquid collecting tank, the membrane washing liquid overflowing from the overflow port is collected and discharged in a centralized manner, thereby making the drainage effect better.

[0035] In the above technical solution, the liquid collecting tank 3 is arranged at the upper end of the corresponding side of the membrane washing tank 1, so that the volume of the liquid collecting tank is relatively small, and it is convenient to set a drain port at its lower end, which is more convenient for connecting pipelines.

[0036] In the above technical solution, there is a height difference between the overflow port 111 and the inner bottom wall of the liquid collecting tank 3, so as to avoid the overflow port being flush with the inner bottom wall of the liquid collecting tank to cause interference in the discharge of liquid.

[0037] like Figure 1-Figure 3As shown, the above technical solution also includes a plurality of partitions 4, and the plurality of partitions 4 are vertically arranged in the upper chamber 11, and the plurality of partitions 4 are parallel to each other in the upper chamber 11 to divide the upper chamber 11 into a plurality of sub-chambers 112 along its length direction, and the support plate 2 is provided with a plurality of seepage holes 21, and each of the sub-chambers 112 corresponds to at least one seepage hole 21, and a plurality of overflow ports 111 are provided, and each of the sub-chambers 112 corresponds to one overflow port 111, so that a plurality of sub-chambers are separated in the upper chamber, so that more membrane materials can be cleaned at the same time without interfering with each other.

[0038] The horizontal height of the upper end of the partition 4 in the above technical solution is lower than the horizontal height of the slot of the membrane washing tank 1, so that a buffer zone is formed in the upper tank chamber above the multiple sub-chambers to prevent any sub-chamber from being full of liquid and overflowing through the slot of the upper tank chamber.

[0039] The width of the sub-chamber 112 in the above technical solution is 2-5 mm, so that the membrane material can stand upright in the sub-chamber, which makes the membrane washing effect better.

[0040] like Figure 2 and Figure 4 As shown, the above technical solution also includes a slot cover 5, the slot cover 5 has a slot facing downward and is detachably buckled on the slot of the membrane washing tank 1, and an air hole 51 is provided on the top wall of the slot cover 5, so that when washing the membrane, the slot cover can be placed on the slot of the membrane washing tank to prevent the membrane washing liquid from shaking out.

[0041] Specifically, the slot height of the liquid collecting tank in this embodiment is lower than the slot height of the membrane washing tank, so that it is convenient to install the slot cover on the slot of the membrane washing tank.

[0042] In this embodiment, the height of the overflow port is lower than the height of the sub-chamber slot.

[0043] In this embodiment, two liquid seepage holes may be provided at the lower end of each sub-chamber.

[0044] In this embodiment, the liquid inlet 121 is arranged at the lower end of the side wall of the membrane washing tank, which is convenient for connecting the pipeline.

[0045] Example 2

[0046] like Figure 5As shown, this embodiment provides an immunoblotting membrane washing assembly, including a liquid storage bottle 20, a waste liquid collection bottle 30, a liquid supply pipe 40, a liquid discharge pipe 50 and the immunoblotting membrane washing device 10 as described in Example 1, wherein the liquid storage bottle 20 is arranged above the immunoblotting membrane washing device 10, one end of the liquid supply pipe 40 is connected to the liquid storage bottle 20, and the other end of the liquid supply pipe 40 is connected to the liquid inlet 121, the waste liquid collection bottle 30 is placed below the immunoblotting membrane washing device 10, one end of the liquid discharge pipe 50 is connected to the liquid discharge port 31, and the other end of the liquid discharge pipe 50 is connected to the waste liquid collection bottle 30, so that the membrane washing liquid in the liquid storage bottle can be stably supplied to the lower chamber through the liquid supply pipe, and the membrane washing liquid to be discharged from the liquid collecting tank is discharged into the waste liquid collection bottle through the liquid discharge pipe, thereby realizing that the membrane washing liquid washes the membrane material in a flowing and renewed state.

[0047] The liquid supply pipe 40 in the above technical solution is a siphon tube, the upper end of which extends into the inner bottom of the liquid storage bottle 20, so that the supply of the membrane washing liquid is more stable.

[0048] When washing the membrane, the immunoblotting membrane washing assembly provided in this embodiment can place the immunoblotting membrane washing device on a cyclotron for oscillation treatment.

[0049] The liquid supply pipe and the liquid discharge pipe described in this embodiment are both hoses.

[0050] It should be noted that the above detailed descriptions are illustrative and intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0051] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0052] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the terms used in this way can be interchangeable where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein, for example.

[0053] Also, when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation.

[0054] In addition, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, product, or apparatus that includes a series of steps or elements is not necessarily limited to those steps or elements explicitly listed, but may include other steps or elements not explicitly listed or inherent to such process, method, product, or apparatus.

[0055] For ease of description, spatially relative terms such as "on", "above", "on the upper surface", "upper", etc. may be used herein to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figures. It should be understood that the spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figures.

[0056] For example, if a device in a drawing is inverted, a device described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above" can encompass both "above" and "below." The device may also be oriented in other different ways (rotated 90 degrees or at other orientations), and the spatially relative descriptors used herein interpreted accordingly.

[0057] The above description is only a preferred embodiment of the utility model and does not limit the utility model in any form. Any ordinary technician in the industry can smoothly implement the utility model as shown in the drawings of the specification and described above. However, any equivalent changes, modifications and evolutions made by technicians familiar with the profession without departing from the scope of the technical solution of the utility model using the technical content disclosed above are all equivalent embodiments of the utility model. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the utility model are still within the protection scope of the technical solution of the utility model.

Claims

1. A membrane washing device for immunoblotting, characterized in that The invention comprises a membrane washing tank (1), wherein the membrane washing tank (1) is arranged vertically with its notch facing upwards, a support plate (2) is horizontally arranged at the inner lower end of the membrane washing tank (1), and the support plate (2) divides the membrane washing tank (1) into an upper tank chamber (11) and a lower chamber (12), wherein the upper tank chamber (11) is located above the lower chamber (12), and the support plate (2) is provided with a seepage hole (21) for connecting the upper tank chamber (11) and the lower chamber (12), the lower end of the membrane washing tank (1) is provided with a liquid inlet (121) connected to the lower chamber (12), and the upper end of the membrane washing tank (1) is provided with an overflow port (111) connected to the upper tank chamber (11).

2. The immunoblotting membrane washing device according to claim 1, characterized in that: It also comprises a liquid collecting trough (3), wherein the liquid collecting trough (3) is arranged on a side corresponding to the length direction of the membrane washing trough (1), and the overflow port (111) is connected to the inside of the liquid collecting trough (3), and the lower end of the liquid collecting trough (3) is provided with a liquid discharge port (31) connected to the inside thereof.

3. The immunoblotting membrane washing device according to claim 2, characterized in that: The liquid collecting tank (3) is arranged at the upper end of the corresponding side of the membrane washing tank (1).

4. The immunoblotting membrane washing device according to claim 3, characterized in that: There is a height difference between the overflow port (111) and the inner bottom wall of the liquid collecting tank (3).

5. The immunoblotting membrane washing device according to claim 2, characterized in that: The invention also comprises a plurality of partitions (4), wherein the plurality of partitions (4) are vertically arranged in the upper tank chamber (11), and the plurality of partitions (4) are mutually parallel in the upper tank chamber (11) so as to divide the upper tank chamber (11) into a plurality of sub-tank chambers (112) along its length direction; the support plate (2) is provided with a plurality of seepage holes (21), and each sub-tank chamber (112) corresponds to at least one seepage hole (21); a plurality of overflow ports (111) are provided, and each sub-tank chamber (112) corresponds to one overflow port (111).

6. The immunoblotting membrane washing device according to claim 5, characterized in that: The level of the upper end of the partition (4) is lower than the level of the notch of the membrane washing tank (1).

7. The immunoblotting membrane washing device according to claim 5, characterized in that: The width of the sub-chamber (112) is 2-5 mm.

8. The immunoblotting membrane washing device according to any one of claims 2 to 7, characterized in that: It also comprises a tank cover (5), the notch of the tank cover (5) is downwardly facing and is detachably buckled on the notch of the membrane washing tank (1), and a ventilation hole (51) is arranged on the top wall of the tank cover (5).

9. An immunoblotting membrane washing assembly, characterized in that: The invention comprises a liquid storage bottle (20), a waste liquid collection bottle (30), a liquid supply pipe (40), a liquid discharge pipe (50) and a membrane washing device (10) for immunoblotting as described in any one of claims 2 to 8, wherein the liquid storage bottle (20) is arranged above the membrane washing device (10) for immunoblotting, one end of the liquid supply pipe (40) is connected to the inside of the liquid storage bottle (20), and the other end of the liquid supply pipe (40) is connected to the liquid inlet (121); the waste liquid collection bottle (30) is placed below the membrane washing device (10), one end of the liquid discharge pipe (50) is connected to the liquid discharge port (31), and the other end of the liquid discharge pipe (50) is connected to the inside of the waste liquid collection bottle (30).

10. The immunoblotting membrane washing assembly according to claim 9, characterized in that: The liquid supply pipe (40) is a siphon tube, the upper end of which extends into the inner bottom of the liquid storage bottle (20).

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