Test tube mixing device
Through technical means such as clamping, rotation, and magnet control of the test tube mixing device, the solution uneven problem caused by nano-magnetic bead sedimentation is solved, and the efficient mixing of magnetic beads and reagents is achieved, which simplifies the operation and reduces the difficulty of automation implementation.
Patent Information
- Application Number
- CN202421847313.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-01
- Publication Date
- 2025-05-27
- Estimated Expiration
- 2034-08-01
AI Technical Summary
In the experiment, nanomagnetic beads are prone to settle, resulting in uneven distribution of magnetic beads in the solution, which affects the use. The existing technology uses permanent magnets to accelerate the aggregation of magnetic beads, but the magnetic beads are difficult to separate and disperse, resulting in complex operation and difficult automation implementation.
A test tube mixing device is provided, including a fixing frame, a clamping assembly, a drive assembly, a lifting assembly, a translation assembly and a magnet. The test tube is clamped by the clamping assembly, and the driving assembly drives the test tube to rotate. The translation assembly drives the magnet to approach or away from the test tube, controls the change of the magnetic field to drive the magnetic beads to achieve a stirring effect, and expands the stirring effect to the entire test tube through the lifting assembly.
The full mixing of magnetic beads and reaction reagents is achieved, the operation process is simplified, the difficulty and cost of realizing automation equipment is reduced, and the work efficiency is improved.
Smart Images
Figure CN222900855U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of experimental instruments, and particularly relates to a test tube mixing device. Background Art
[0002] In many fields of biomedicine, nano magnetic beads are needed. However, nano magnetic beads have a certain density and mass, and are prone to sedimentation, resulting in uneven distribution of magnetic beads in the solution, which in turn affects the use. Therefore, in the process of use, in order to maintain the uniformity of the magnetic bead solution, it is necessary to continuously mix the magnetic bead solution.
[0003] In the prior art, in order to enable the magnetic beads suspended in the experimental liquid to be collected on the tube wall of the test tube more quickly each time the test tube is left standing, a permanent magnet is used to apply a magnetic field force to accelerate the aggregation of the magnetic beads, so as to accelerate the collection of the magnetic beads on the tube wall of the test tube. However, after the permanent magnet applies a magnetic field force to accelerate the aggregation of the magnetic beads, even if the permanent magnet is removed, the residual magnetic stress of the magnetic beads causes the magnetic beads to attract and aggregate with each other and will not automatically separate from each other. After pouring out the old experimental liquid and pouring in the new experimental liquid, simply shaking the test tube still cannot completely separate and disperse all the magnetic beads. It is necessary to fully stir the experimental liquid and shake it sufficiently at the same time to completely separate and disperse it, and then continue to complete the subsequent experimental operations, which leads to the complexity of the operation of the magnetic beads. Especially in the realization of automated equipment, it causes the difficulty and high cost of automation.
[0004] Outside the instrument, manual pipetting and mixing are mostly used for operation, which not only affects the efficiency, consumes energy, but also may cause unnecessary reagent contamination; sometimes vortex oscillation mixing is also used, but both require interrupting the experimental process and affect the work efficiency. Content of the Utility Model
[0005] The utility model provides a test tube mixing device, aiming to solve the technical problem that the magnetic beads are difficult to separate and disperse after being aggregated by a permanent magnet during the experiment in the prior art.
[0006] To achieve the above object, the technical solution adopted by the utility model is: to provide a test tube mixing device, including:
[0007] A fixing frame, fixedly arranged;
[0008] A clamping assembly, rotatably matched with the fixing frame, used for clamping the test tube;
[0009] A first driving assembly, connected to the fixing frame, and the power output end is connected to the clamping assembly, used for driving the clamping assembly to rotate;
[0010] The lifting assembly includes a second driving assembly and a lifting frame. The lifting frame is slidably engaged with the fixed frame and has the freedom to slide in the vertical direction. The second driving assembly is connected to the fixed frame, and the power output end is connected to the lifting frame to drive the lifting frame to move up and down.
[0011] The translation assembly includes a third driving assembly and a translation frame. The translation frame is slidably engaged with the lifting frame and has the freedom to slide horizontally. The third driving assembly is connected to the lifting frame, and the power output end is connected to the translation frame to drive the translation frame to slide horizontally. And
[0012] The magnet is provided on one side of the clamping assembly and is connected to the translation frame. The magnet is adapted to move up and down along the test tube on the clamping assembly and approach or move away from the test tube under the drive of the lifting assembly and the translation assembly.
[0013] In a possible implementation manner of the test tube mixing device provided by the present utility model, the clamping assembly includes a plurality of clamping mechanisms. The plurality of clamping mechanisms are spaced on the fixed frame and are rotatably engaged with the fixed frame. Each clamping mechanism is used to clamp a test tube. The magnets are provided in plurality, and the magnets correspond to the clamping mechanisms one by one.
[0014] In a possible implementation manner of the test tube mixing device provided by the present utility model, the clamping mechanism includes:
[0015] A rotating shaft, which is rotatably engaged with the fixed frame. A clamping groove is provided at the top of the rotating shaft, and a threaded structure is provided on the outer side. The clamping groove is adapted to place a test tube; and
[0016] A compression nut, with an avoidance groove provided at the center. The avoidance groove is used to avoid the test tube. The compression nut is threadedly engaged with the threaded structure on the rotating shaft.
[0017] In a possible implementation manner of the test tube mixing device provided by the present utility model, a water passage is axially provided in the rotating shaft, and the water passage is communicated with the clamping groove;
[0018] The clamping mechanism further includes:
[0019] A sealing ring, which is provided in the clamping groove. The sealing ring is sealingly engaged with the inner wall of the clamping groove and is adapted to be sealingly engaged with the test tube; and
[0020] A connector, which is connected to the lower end of the rotating shaft and is communicated with the water passage.
[0021] In a possible implementation manner of the test tube mixing device provided by the present utility model, the clamping mechanism further includes a driven gear, the driven gear is connected to the rotating shaft, and the driven gears on two adjacent clamping mechanisms are meshed with each other;
[0022] The first driving component includes:
[0023] A first motor component, connected to the fixed frame; and
[0024] A driving gear, connected to the power output end of the first motor component and meshed with one of the driven gears.
[0025] In a possible implementation manner of the test tube mixing device provided by the present utility model, the second driving component is an electric push rod, the electric push rod is connected to the fixed frame, and the power output end is connected to the lifting frame.
[0026] In a possible implementation manner of the test tube mixing device provided by the present utility model, the third driving component includes:
[0027] A third motor component, connected to the lifting frame; and
[0028] A translation screw rod, in threaded cooperation with the translation frame, and one end of the translation screw rod is connected to the power output end of the third motor component.
[0029] The beneficial effects of the test tube mixing device provided by the present utility model are as follows: Compared with the prior art, after adding reaction reagents to the test tube, the test tube mixing device provided by the present utility model clamps the test tube through the clamping component and drives the test tube to rotate through the first driving component. During this process, the magnet is driven by the translation component to approach and move away from the test tube to control the magnetic field change of the position of the magnetic beads, and the magnetic beads in the test tube are driven to move through the magnetic field change to achieve the stirring effect. The magnet is driven by the lifting component to move up and down along the test tube, and the stirring effect formed by the magnetic field change is extended to the whole test tube, so that the magnetic beads and the reaction reagents are fully mixed. Description of the Drawings
[0030] Figure 1 Schematic diagram of the three-dimensional structure of the test tube mixing device provided by the embodiment of the present utility model Figure 1 ;
[0031] Figure 2 Schematic diagram of the three-dimensional structure of the test tube mixing device provided by the embodiment of the present utility model Figure 2 ;
[0032] Figure 3 Exploded structure schematic diagram of the clamping mechanism in the test tube mixing device provided by the embodiment of the present utility model;
[0033] Figure 4This is a cross-sectional structural view of a test tube in an embodiment of the present utility model;
[0034] Explanation of reference numerals in the drawings:
[0035] 10, fixing bracket; 21, rotating shaft; 22, compression nut; 23, sealing ring;
[0036] 24, connecting head; 25, driven gear; 31, first motor assembly; 32, driving gear;
[0037] 41, electric push rod; 42, lifting bracket; 51, third motor assembly; 52, translation screw;
[0038] 53, translation bracket; 60, magnet; 70, test tube; 71, filter element. Specific implementation manners
[0039] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present utility model clearer and more understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.
[0040] Next, the technical solutions in the embodiments of the present application will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The following description of at least one exemplary embodiment is actually illustrative only and in no way limits the present application and its application or use. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.
[0041] It should be noted that the terms used herein are only for describing specific implementation manners and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or their combinations.
[0042] Unless otherwise specifically stated, the relative arrangements of components and steps, numerical expressions, and numerical values set forth in these embodiments do not limit the scope of the present application. At the same time, it should be understood that for the sake of convenience of description, the dimensions of the various parts shown in the drawings are not drawn in actual proportional relationship. Technologies, methods, and devices known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the said technologies, methods, and devices should be regarded as part of the description. In all the examples shown and discussed here, any specific value should be construed as merely exemplary and not as a limitation. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that like reference numerals and letters denote like items in the following drawings, and thus, once an item is defined in one drawing, further discussion thereof is not required in subsequent drawings.
[0043] In the description of the present application, it should be understood that the orientation or positional relationships indicated by orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, perpendicular, horizontal", and "top, bottom", etc. are generally based on the orientation or positional relationships shown in the drawings. It is only for the convenience of describing the present application and simplifying the description. Without contrary explanation, these orientation words do not indicate and imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation. Therefore, it should not be construed as limiting the protection scope of the present application; the orientation words "inside, outside" refer to the inside and outside relative to the contour of each component itself.
[0044] For the convenience of description, spatial relative terms such as "above...", "over...", "on the upper surface of...", "above-mentioned", etc. can be used here to describe the spatial positional relationships between a device or feature shown in the drawings and other devices or features. It should be understood that the spatial relative terms are intended to include different orientations in use or operation in addition to the orientation described in the drawings for the device. For example, if the device in the drawing is inverted, the device described as "above other devices or structures" or "over other devices or structures" will then be positioned "below other devices or structures" or "under other devices or structures". Thus, the exemplary term "above..." can include both the orientations of "above..." and "below...". The device can also be positioned in other different ways, and corresponding interpretations should be made for the spatial relative descriptions used here.
[0045] In addition, it should be noted that the use of words such as "first", "second", etc. to limit components is only for the convenience of distinguishing the corresponding components. Without additional statement, the above words have no special meaning. Therefore, it should not be construed as limiting the protection scope of the present application.
[0046] Please refer to Figures 1 to 4, the test tube mixing device provided by the present utility model will now be described. The test tube mixing device includes a fixed frame 10, a clamping assembly, a first driving assembly, a lifting assembly, a translation assembly, and a magnet 60. The fixed frame 10 is fixedly arranged; the clamping member is rotatably matched with the fixed frame 10 and is used to clamp the test tube 70; the first driving assembly is connected to the fixed frame 10, and the power output end is connected to the clamping assembly to drive the clamping assembly to rotate; the lifting assembly includes a second driving assembly and a lifting frame 42. The lifting frame 42 is slidably matched with the fixed frame 10 and has the freedom to slide in the vertical direction; the second driving assembly is connected to the fixed frame 10, and the power output end is connected to the lifting frame 42 to drive the lifting frame 42 to move up and down; the translation assembly includes a third driving assembly and a translation frame 53. The translation frame 53 is slidably matched with the lifting frame 42 and has the freedom to slide horizontally; the third driving assembly is connected to the lifting frame 42, and the power output end is connected to the translation frame 53 to drive the translation frame 53 to slide horizontally; the magnet 60 is arranged on one side of the clamping assembly and is connected to the translation frame 53. The magnet 60 is adapted to move up and down along the test tube 70 on the clamping assembly and approach or move away from the test tube 70 under the drive of the lifting assembly and the translation assembly.
[0047] The beneficial effect of the test tube 70 mixing device provided by the present utility model is that, compared with the prior art, after adding reaction reagents to the test tube 70, the test tube 70 is clamped by the clamping assembly, and the test tube 70 is driven to rotate by the first driving assembly. During this process, the magnet 60 is driven by the translation assembly to approach and move away from the test tube 70 to control the magnetic field change at the position of the magnetic beads. The magnetic beads in the test tube 70 are driven to move through the magnetic field change to achieve the stirring effect. The magnet 60 is driven by the lifting assembly to move up and down along the test tube 70, and the stirring effect formed by the magnetic field change is extended to the whole test tube 70, so that the magnetic beads and the reaction reagents are fully mixed.
[0048] As Figure 1 and Figure 2 shown, in a specific implementation manner of the test tube 70 mixing device provided by the embodiment of the present utility model, the clamping assembly includes a plurality of clamping mechanisms. The plurality of clamping mechanisms are arranged on the fixed frame 10 at intervals and are rotatably matched with the fixed frame 10. Each clamping mechanism is used to clamp the test tube 70; there are a plurality of magnets 60, and the magnets 60 correspond to the clamping mechanisms one by one to process multiple test tubes 70 at the same time, greatly improving the efficiency.
[0049] As Figure 1 and Figure 3As shown, in a specific embodiment of the test tube 70 mixing device provided by the embodiment of the present utility model, the clamping mechanism includes a rotating shaft 21 and a compression nut 22. The rotating shaft 21 is rotationally matched with the fixed frame 10. A clamping groove is provided at the top of the rotating shaft 21, and a threaded structure is provided on the outside. The clamping groove is adapted to place the test tube 70; a relief groove is provided at the center of the compression nut 22 for avoiding the test tube 70, and the compression nut 22 is in threaded cooperation with the threaded structure on the rotating shaft 21.
[0050] As Figure 1 and Figure 3 As shown, in a specific embodiment of the test tube 70 mixing device provided by the embodiment of the present utility model, a water passage is axially provided in the rotating shaft 21, and the water passage is communicated with the clamping groove; the clamping mechanism further includes a sealing ring 23 and a connector 24. The sealing ring 23 is provided in the clamping groove, and the sealing ring 23 is in sealing cooperation with the inner wall of the clamping groove and is adapted to be in sealing cooperation with the test tube 70; the connector 24 is connected to the lower end of the rotating shaft 21 and is communicated with the water passage.
[0051] Specifically, as Figure 4 shown, the bottom of the test tube 70 is provided with an opening and is communicated with the clamping groove. A filter element 71 is provided in the test tube 70, and the reaction reagent is placed above the filter element 71. Before draining, the lower side of the filter element 71 is sealed, and the air does not circulate. Coupled with the resistance of the filter element 71, the reaction reagent is kept above the filter element 71.
[0052] It should be noted that during the liquid drainage stage, the negative pressure pipeline is communicated with the connector 24 to drain the reagent in the test tube 70. During this process, the magnetic beads are adsorbed on the side wall of the test tube 70 by the magnet 60 to prevent the magnetic beads from being drained away with the liquid. Therefore, the device provided by this embodiment can mix and adsorb the magnetic beads under the same working station, without the need to change the working station back and forth, reducing the volume of the device; the device can realize continuous reaction, improve the working efficiency, and make it possible to automate the reaction process.
[0053] As Figure 1 and Figure 2 shown, in a specific embodiment of the test tube 70 mixing device provided by the embodiment of the present utility model, the clamping mechanism further includes a driven gear 25. The driven gear 25 is connected to the rotating shaft 21, and the driven gears 25 on two adjacent clamping mechanisms are meshed with each other; a first driving component includes a first motor component 31 and a driving gear 32. The first motor component 31 is connected to the fixed frame 10; the driving gear 32 is connected to the power output end of the first motor component 31 and is meshed with a driven gear 25. By driving one of the driven gears 25 to rotate through the first motor component 31, the power can be transmitted to all the driven gears 25, and then all the clamping mechanisms are driven to rotate.
[0054] As Figure 1 andFigure 2 As shown in Figure 2 , in a specific embodiment of the test tube 70 mixing device provided by the present utility model, the second driving assembly is an electric push rod 41. The electric push rod 41 is connected to the fixed frame 10, and the power output end is connected to the lifting frame 42.
[0055] It should be noted that the electric push rod 41 can also be replaced by mechanisms such as hydraulic cylinders, air cylinders, motors, and screws driven by motors that can output axial displacement.
[0056] Specifically, guide rods are provided on the lifting frame 42, and guide sleeves cooperating with the guide rods are provided on the fixed frame 10. Through the cooperation of the guide rods and the guide sleeves, stable sliding cooperation between the lifting frame 42 and the fixed frame 10 is achieved.
[0057] As Figure 1 and Figure 2 shown in Figure 1 and Figure 2 , in a specific embodiment of the test tube 70 mixing device provided by the present utility model, the third driving assembly includes a third motor assembly 51 and a translation screw 52. The third motor assembly 51 is connected to the lifting frame 42; the translation screw 52 is in threaded cooperation with the translation frame 53, and one end of the translation screw 52 is connected to the power output end of the third motor assembly 51.
[0058] It should be noted that the third driving assembly can also be replaced by mechanisms such as hydraulic cylinders, air cylinders, and the electric push rod 41 that can output axial displacement.
[0059] Specifically, guide rods are provided on the translation frame 53, and guide sleeves cooperating with the guide rods are provided on the lifting frame 42. Through the cooperation of the guide rods and the guide sleeves, stable sliding cooperation between the lifting frame 42 and the translation frame 53 is achieved.
[0060] The usage steps of the test tube 70 mixing device provided by the present utility model are as follows:
[0061] S01, Put the reaction reagent into the test tube 70. The first driving assembly drives the clamping mechanism to rotate, thereby driving the test tube 70 to rotate. The magnetic beads will have a velocity along the rotation tangent direction and the suction force of the permanent magnet 60. When the magnetic beads rotate away from the permanent magnet 60, this part of the magnetic beads will be sucked back due to the magnetic force of the permanent magnet 60. When the rotation speed of the test tube 70 and the magnetic force value of the permanent magnet 60 match, the magnetic beads will be sucked back when they are farthest from the permanent magnet 60. At this time, the magnetic beads can fully contact the reagent in the current magnet 60 height range;
[0062] S02, The magnet 60 makes an up-and-down reciprocating motion under the drive of the lifting assembly, which is responsible for expanding the mixing state in the plane area of step S01 to the height level of all reaction reagents, so that the magnetic beads can form a vortex motion in the reaction reagent, and the magnetic beads can be distributed in all areas of the reaction reagent;
[0063] S03. When the magnetic beads and the reaction reagent in the test tube 70 reach a well-mixed state, the translation assembly starts to intervene. The translation assembly drives the magnet 60 away from the test tube 70, so that the magnetic beads are in a non-magnetic or weak magnetic field and maintain the well-mixed state. This step can prevent the magnetic beads from adhering to the wall or aggregating into clusters under the persistent magnetic force of the magnet 60.
[0064] S04. After the magnet 60 moves away from the test tube 70 for a period of time, the magnetic beads will naturally settle and aggregate at the bottom of the test tube 70. At this time, the magnet 60 translation motor and the magnet 60 lifting motor jointly drive the magnet 60 to approach the magnetic beads, and repeat the above steps to make the magnetic beads come into full contact with the reaction reagent again.
[0065] S05. When it is necessary to drain the current reaction reagent at the end of the reaction, the lifting assembly and the translation assembly drive the magnet 60 to approach the test tube 70, adsorb the magnetic beads at an appropriate position on the wall of the test tube 70, and evacuate the current reaction reagent. Since the magnetic beads are adsorbed by the magnet 60 close to the side wall of the test tube 70, the loss of the magnetic beads is avoided.
[0066] S06. Add new reaction reagent again, and repeat the above mixing steps and adsorption and drainage steps to achieve continuous reaction of the magnetic beads in the test tube 70.
[0067] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A test tube mixing device, characterized in that: include: Fixed frame, fixed setting; A clamping assembly, rotatably matched with the fixing frame, for clamping the test tube; A first driving assembly is connected to the fixing frame, and a power output end is connected to the clamping assembly, so as to drive the clamping assembly to rotate; The lifting assembly comprises a second driving assembly and a lifting frame, wherein the lifting frame is slidably matched with the fixed frame and has the freedom to slide in the vertical direction; the second driving assembly is connected to the fixed frame, and a power output end is connected to the lifting frame to drive the lifting frame to move up and down; The translation assembly comprises a third driving assembly and a translation frame, wherein the translation frame is slidably matched with the lifting frame and has a degree of freedom of lateral sliding; the third driving assembly is connected to the lifting frame, and a power output end is connected to the translation frame, so as to drive the translation frame to slide lateraly; as well as A magnet is arranged at one side of the clamping assembly and connected to the translation frame. The magnet is suitable for moving up and down, approaching or moving away from the test tube on the clamping assembly under the drive of the lifting assembly and the translation assembly.
2. The test tube mixing device according to claim 1, characterized in that: The clamping assembly comprises a plurality of clamping mechanisms, which are arranged on the fixing frame at intervals and rotatably cooperate with the fixing frame, and each clamping mechanism is used to clamp a test tube; the magnets are a plurality of pieces, and the magnets correspond to the clamping mechanisms one by one.
3. The test tube mixing device according to claim 2, characterized in that: The clamping mechanism comprises: A rotating shaft, rotatably matched with the fixing frame, a clamping groove is provided on the top of the rotating shaft, and a threaded structure is provided on the outer side, and a test tube is suitable for being placed in the clamping groove; and The center of the clamping nut is provided with an escape groove, the escape groove is used to escape the test tube, and the clamping nut is threadably matched with the thread structure on the rotating shaft.
4. The test tube mixing device according to claim 3, characterized in that: A water passage is provided in the rotating shaft along the axial direction, and the water passage is communicated with the clamping groove; The clamping mechanism further comprises: A sealing ring is disposed in the clamping groove, the sealing ring is in sealing cooperation with the inner wall of the clamping groove and is suitable for sealing cooperation with the test tube; and A connector is connected to the lower end of the rotating shaft and communicated with the water passage.
5. The test tube mixing device according to claim 3, characterized in that: The clamping mechanism further comprises a driven gear, the driven gear is connected to the rotating shaft, and the driven gears on two adjacent clamping mechanisms are meshed; The first driving component comprises: A first motor assembly connected to the fixing frame; and A driving gear is connected to the power output end of the first motor assembly and meshes with one of the driven gears.
6. The test tube mixing device according to claim 1, characterized in that: The second driving component is an electric push rod, the electric push rod is connected to the fixing frame, and a power output end is connected to the lifting frame.
7. The test tube mixing device according to claim 1, characterized in that: The third drive assembly includes: A third motor assembly connected to the lifting frame; and The translation screw rod is threadably matched with the translation frame, and one end of the translation screw rod is connected to the power output end of the third motor assembly.